DEAS 232 :2019  
ICS 65.120   
 
© EA C 2019                                                                                                                               2nd Edition 2019   
 DRAFT EAST AFRICAN STANDARD  
Maize gluten  for animal  feed — Specification   
EAST AFRICAN COMMUNITY  
 DEAS 232 : 2019  
ii © EAC 2019  – All rights reserved  
 Copyright notice  
This EAC document is copyright -protected by EAC. While the reproduction of this document by 
participants in the EAC standards development pr ocess is permitted without prior permission from EAC, 
neither this document nor any extract from it may be reproduced, stored or transmitted in any form for 
any other purpose without prior written permission from EAC.  
Requests for permission to reproduce t his document for the purpose of selling it should be addressed 
as shown below or to EAC’s member body in the country of the requester:  
© East African Community  2019  — All rights reserved  
East African Community   
P.O. Box 1096 , 
Arusha  
Tanzania  
Tel: + 255 27 2162100  
Fax: + 255 27 2162190  
E-mail: eac@eachq.org  
       Web: www.eac -quality.net  
Reproduction for sales purposes may be subject to royalty payments or a licensing agreement.  
Violators may be prosecuted . 
 DEAS 232: 2019  
© EAC 2019  – All rights reserved  iii 
 Foreword  
Development of the East African Standard s has been necessitated by the need for harmonizing requirements 
governing quality of products and services in the East African Community. It is envisaged that through 
harmonized standardization, trade barriers that are encountered when goods and services are exchanged 
within the Community will be removed.  
The Community has established an East African Standards Committee  (EASC)  mandated to develop and 
issue East African Standards  (EAS) . The Committee is composed of representatives of the National 
Standards  Bodies in Partner States, together with the representatives from the public and private sector 
organizations  in the community .  
East African Standards are developed through  Technical Committees that are representative of key 
stakeholders including governm ent, academia, consumer groups, private sector and other interested parties. 
Draft East African Standards are circulated to stakeholders through the National Standards Bodies in the 
Partner States. The comments received are discussed and incorporated befor e finalization of standards, in 
accordance with the Principles and procedures for development of East African Standards.  
East African Standards are subject to review, to keep pace with technological advances. Users of the East 
African Standards are therefo re expected to ensure that they always have the latest versions of the standards 
they are implementing .  
The committee responsible  for this document is Technical Committee EASC /TC 001, Animal feeds and 
feeding stuffs . 
Attention is drawn to the possibility that some of the elements of this document may be subject of patent 
rights. EAC shall not be held responsible for identifying any or all such patent rights.  
This second  edition cancels and replaces the first edition ( EAS232:2001), which has been technicall y revised.  
 DRAFT EAST AFRICAN STANDARD  DEAS 232 :2019  
 
© EAC 2019  – All rights  reserved  1 
 Maize gluten for animal feed — Specification  
1 Scope   
This Draft East African Standard specifies requirements, sampling and test methods for maize gluten meal and 
feed for use in animal feeds . 
2 Normative references   
The following documents ar e referred to in the text in such a way that some or all of their content constitutes 
requirements of this document.  For dated references, only the edition cited applies. For undated references, 
the latest edition of the referenced document (including any amendments) applies.  
IS0 5510, Animal feeding stuffs — Determination of available lysine  
ISO 5983 -1, Animal feeding stuffs — Determination of nitrogen content and calculation of crude protein 
content — Part 1: Kjeldahl method  
ISO 5984, Animal feeding stuff s — Determination of crude ash  
ISO 5985, Animal feeding stuffs — Determination of ash insoluble in hydrochloric acid  
ISO 6490 -1, Animal feeding stuffs — Determination of calcium content — Part 1: Titrimetric method  
ISO 6491, Animal feeding stuffs — Determi nation of phosphorus content — Spectrometric method  
ISO 6492, Animal feeding stuffs — Determination of fat content  
ISO 6495, Animal feeding stuffs — Determination of water -soluble chlorides content  
ISO 6496, Animal feeding stuffs — Determination of moistur e and other volatile matter content  
ISO 6497, Animal feeding stuffs — Sampling  
ISO 6865, Animal feeding stuffs — Determination of crude fibre content — Method with intermediate filtration  
ISO 9831, Animal feeding stuffs, animal products, and faeces or urin e — Determination of gross calorific value 
— Bomb calorimeter method  
ISO 13903, Animal feeding stuffs — Determination of amino acids content  
ISO 14718, Animal feeding stuffs — Determination of aflatoxin B1 content of mixed feeding stuffs — Method 
using hig h-performance liquid chromatography  
ISO 17375, Animal feeding stuffs — Determination of aflatoxin B1  
ISO 16050 , Foodstuffs — Determination of aflatoxin B1, and the total content of aflatoxins B1, B2, G1 and G2 
in cereals, nuts and derived products — High-performance liquid chromatographic method   
2 © EAC 2019  – All rights reserved  
 ISO 27085, Animal feeding stuff — Determination of calcium, sodium, phosphorous, magnesium, potassium, 
iron, zinc, copper, manganese, cobalt, molybdenum, arsenic, lead and cadmium by ICP -AES 
EAS 38, Labelling of pre -packaged foods — General requirements  
3 Terms and definitions   
For the purposes of this document, the following terms and definitions apply.  
ISO and IEC maintain terminological databases for use in standardization at the following addresses:  
— ISO Onlin e browsing platform: available at http://www.iso.org/obp  
3.1  
maize gluten meal  
product derived from the wet milling process of starch manufacture and is mainly composed of proteins, 
carbohydrates and minerals  
3.2  
maize gluten feed  
product derived from the wet milling process of starch manufacture and is mainly composed of the fibrous part 
of the maize and soluble proteins starch and minerals. Its essentially fed to ruminants and is highly digested 
by ruminants   
3.3  
extraneous matter  
organic or inorgani c materials other than maize bran such as  sand, stones, chaff, dead insects and others.   
4 Requirements  
4.1 General requirements  
Maize gluten feed shall : 
a) be in the form of a meal  or pellets or granules ; 
b) free from rancidity and from fermented, musty or othe r objectionable odour ; and  
c) be free from any extraneous matter , adulterants, harmful consituents, insects or fungus infestation . 
4.2 Specific requirements   
The material shall conform  to the requirements specified in Table 1 when tested in accordance with th e test 
methods specified therein . 
 
 
 
 
  
© EAC 2019  – All rights reserved  3 
 Table 1 — Requirements for maize gluten feed  
 
S/No Characteristic  Requirement  Test method  
Meal  Feed  
I Moisture, per cent by mass, max.  12 12 ISO 6496  
ii Crude fat, per cent by mass, min.a 1 1 ISO 6492  
iii Crud e protein (N x 6.25), per cent by mass, mina 55 20 ISO 5983 -1 
iv Crude fibre, per cent by mass, max.a 3 12 ISO 6865  
v Acid insoluble ash, per cent by mass, max.  0.4 0.4 ISO 5985  
a Characteristic  is on dry matter  basis . 
 
5 Contaminants  
5.1 Aflatoxin  
Aflatoxin contamination in maize gluten shall not be more than 10 ppb  for B 1 and 20  ppb for total aflatoxin  
when tested with ISO 16050 . 
5.2 Pesticide  residues  
Maize gluten shall comply with those maximum limits for pesticide residues stated in Codex Alimenta rius 
commission  
5.3 Heavy metal  
Maize gluten shall be free from heavy metals in amounts which may represent a hazard to animals and shall 
comply with the maximum limits of heavy metals as specified in Table 3 when tested in accordance with the 
methods spe cified therein  
Table 2 — Limits for heavy metals in maize gluten  
S/No Heavy metal  Maximum limit,  
mg/kg Test method  
1 Arsenic  2.0  
ISO 27085  2 Lead  5.0 
3 Cadmium  1.0 
 
6 Hygiene  
Maize gluten  shall be prepared in accordance with CAC/RCP 54 . 
7 Weights  and measures  
Maize gluten  shall be weighed according to the weights and measures regulation of the destination country .  
4 © EAC 2019  – All rights reserved  
 8 Packaging  
Maize gluten  feeds for sale shall be packaged in suitable containers that are of sufficient strength, and 
sufficiently sea led to withstand reasonable handling without tearing, bursting or falling open. The containers 
shall be clean and not previously used . 
9 Labe lling  
In addition to the requirements in EAS 38, each package shall be legibly and indelibly labelled with the 
following:   
a) expiry date ; 
b) name of the feed for example  ― Maize gluten ‖; 
c) name and physical address  of the manufacturer ; 
d) net weight in metric units;  
e) batch number /lot identification;  
f) manufacturing date ; and  
g) storage instru ctions . 
10 Sampling  
Sampling shall be do ne in accordance ISO 6497 .  
© EAC 2019  – All rights reserved  5 
 Bibliography  
EAS 232:2001, Maize gluten animal feeds  specification  
 EAS 232: 2019    
 
© EAC  2019  – All rights reserved