PUBLIC REVIEW DRAFT DRAFT UGANDA STANDARD  
  DUS DEAS  232 
Second   Edition  
2019-mm-dd 
 
 Reference number  
DUS DEAS  232: 2019 
© UNBS 2019 
   
Maize gluten for animal feed — Specification  
 
 PUBLIC REVIEW DRAFTDUS DEAS 232: 2019 
ii                © UNBS  2019 - All rights reserved 
 Compliance with this standard does not, of itself confer immunity from legal obligations 
A Uganda Standard does not purport to include all necessary provisions of a contract. Users are 
responsible for its correct application  
© UNBS 2019 
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilised in any 
form or by any means, electronic or mechanical, including photocopying and microfilm, without prior written 
permission from UNBS. 
Requests for permission to reproduce this document should be addressed to 
The Executive Director 
Uganda National Bureau of Standards 
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Uganda 
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E-mail: info@unbs.go.ug  
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 PUBLIC REVIEW DRAFTDUS DEAS 232: 2019 
© UNBS  2019 - All rights reserved                iii National foreword 
Uganda National Bureau of Standards (UNBS) is a parastatal under the Ministry of Trade, Industry and 
Cooperatives established under Cap 327, of the Laws of Uganda, as amended.  UNBS is mandated to co-
ordinate the elaboration of standards and is  
(a) a member of International Organisation for Standardisation (ISO) and  
(b) a contact point for the WHO/FAO Codex Alimentarius Commission on Food Standards, and  
(c) the National Enquiry Point on TBT Agreement of the World Trade Organisation (WTO). 
The work of preparing Uganda Standards is carried out through Technical Committees. A Technical 
Committee is established to deliberate on standards in a given field or area and consists of representatives of 
consumers, traders, academicians, manufacturers, government and other stakeholders. 
Draft Uganda Standards adopted by the Technical Committee are widely circulated to stakeholders and the 
general public for comments. The committee reviews the comments before recommending the draft standards 
for approval and declaration as Uganda Standards by the National Standards Council.  
This Draft Uganda Standard, DUS DEAS 232: 2019 , Maize gluten for animal feed  — Specification , is identical 
with and has been reproduced from an East African Standard, DEAS 232 : 2019, Maize gluten for animal feed   
— Specification , and is being proposed for adoption as a Uganda Standard. 
The committee responsible for this document is Technical Committee UNBS/TC 2,  Food and agriculture , 
Subcommittee SC 9, Animal feeds and feeding stuffs.  
This second edition cancels and replaces the first edition (US EAS 232:2001), which has been technically 
revised. 
Wherever the words, “ East African Standard " appear, they should be replaced by "Uganda Standard." 
 
 
 
 
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 DEAS 232 :2019  
ICS 65.120   
 
© EA C 2019                                                                                                                              2nd Edition 2019  
 DRAFT EAST AFRICAN STANDARD 
Maize gluten for animal feed — Specification  
EAST AFRICAN COMMUNITY 
 PUBLIC REVIEW DRAFTPUBLIC REVIEW DRAFTDEAS 232: 2019 
ii © EAC 2019  – All rights reserved  
 Copyright notice 
This EAC document is copyright-protected by EAC. While the reproduction of this document by 
participants in the EAC standards development process is permitted without prior permission from EAC, 
neither this document nor any extract from it may be reproduced, stored or transmitted in any form for 
any other purpose without prior written permission from EAC. 
Requests for permission to reproduce this document for the purpose of selling it should be addressed 
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© East African Community 2019  — All rights reserved 
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Reproduction for sales purposes may be subject to royalty payments or a licensing agreement. 
Violators may be prosecuted. 
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© EAC 2019  – All rights reserved  iii 
 Foreword 
Development of the East African Standards has been necessitated by the need for harmonizing requirements 
governing quality of products and services in the East African Community. It is envisaged that through 
harmonized standardization, trade barriers that are encountered when goods and services are exchanged 
within the Community will be removed.  
The Community has established an East African Standards Committee (EASC) mandated to develop and 
issue East African Standards (EAS).  The Committee is composed of representatives of the National 
Standards Bodies in Partner States, together with the representatives from the public and private sector 
organizations in the community .  
East African Standards are developed through Technical Committees that are representative of key 
stakeholders including government, academia, consumer groups, private sector and other interested parties. 
Draft East African Standards are circulated to stakeholders through the National Standards Bodies in the 
Partner States. The comments received are discussed and incorporated before finalization of standards, in 
accordance with the Principles and procedures for development of East African Standards. 
East African Standards are subject to review, to keep pace with technological advances. Users of the East 
African Standards are therefore expected to ensure that they always have the latest versions of the standards 
they are implementing.  
The committee responsible  for this document is Technical Committee EASC/TC 001, Animal feeds and 
feeding stuffs . 
Attention is drawn to the possibility that some of the elements of this document may be subject of patent 
rights. EAC shall not be held responsible for identifying any or all such patent rights. 
This second  edition cancels and replaces the first edition (EAS 232:2001 ), which has been technically revised.  
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© EAC 2019  – All rights  reserved  1 
 Maize gluten for animal feed — Specification 
1 Scope  
This Draft East African Standard specifies requirements, sampling and test methods for maize gluten meal and 
feed for use in animal feeds . 
2 Normative references  
The following documents are referred to in the text in such a way that some or all of their content constitutes 
requirements of this document. For dated references, only the edition cited applies. For undated references, 
the latest edition of the referenced document (including any amendments) applies. 
IS0 5510, Animal feeding stuffs — Determination of available lysine 
ISO 5983- 1, Animal feeding stuffs — Determination of nitrogen content and calculation of crude protein 
content — Part 1: Kjeldahl method 
ISO 5984, Animal feeding stuff s — Determination of crude ash 
ISO 5985, Animal feeding stuffs — Determination of ash insoluble in hydrochloric acid 
ISO 6490-1, Animal feeding stuffs — Determination of calcium content — Part 1: Titrimetric method 
ISO 6491, Animal feeding stuffs — Determination of phosphorus content — Spectrometric method 
ISO 6492, Animal feeding stuffs — Determination of fat content 
ISO 6495, Animal feeding stuffs — Determination of water-soluble chlorides content 
ISO 6496, Animal feeding stuffs — Determination of moisture and other volatile matter content 
ISO 6497, Animal feeding stuffs — Sampling 
ISO 6865, Animal feeding stuffs — Determination of crude fibre content — Method with intermediate filtration  
ISO 9831, Animal feeding stuffs, animal products, and faeces or urin e — Determination of gross calorific value 
— Bomb calorimeter method 
ISO 13903, Animal feeding stuffs — Determination of amino acids content 
ISO 14718, Animal feeding stuffs — Determination of aflatoxin B1 content of mixed feeding stuffs — Method 
using high-performance liquid chromatography 
ISO 17375, Animal feeding stuffs — Determination of aflatoxin B1 
ISO 16050 , Foodstuffs — Determination of aflatoxin B1, and the total content of aflatoxins B1, B2, G1 and G2 
in cereals, nuts and derived products — High-performance liquid chromatographic method PUBLIC REVIEW DRAFT 
2 © EAC 2019  – All rights reserved  
 ISO 27085, Animal feeding stuff — Determination of calcium, sodium, phosphorous, magnesium, potassium, 
iron, zinc, copper, manganese, cobalt, molybdenum, arsenic, lead and cadmium by ICP-AES 
EAS 38, Labelling of pre-packaged foods — General requirements  
3 Terms and definitions  
For the purposes of this document, the following terms and definitions apply.  
ISO and IEC maintain terminological databases for use in standardization at the following addresses:  
— ISO Online browsing platform: available at http://www.iso.org/obp  
3.1  
maize gluten meal 
product derived from the wet milling process of starch manufacture and is mainly composed of proteins, 
carbohydrates and minerals  
3.2  
maize gluten feed  
product derived from the wet milling process of starch manufacture and is mainly composed of the fibrous part 
of the maize and soluble proteins starch and minerals. Its essentially fed to ruminants and is highly digested 
by ruminants  
3.3  
extraneous matter 
organic or inorganic materials other than maize bran such as sand, stones, chaff, dead insects and others.  
4 Requirements 
4.1 General requirements 
Maize gluten feed shall: 
a) be in the form of a meal or pellets or granules; 
b) free from rancidity and from fermented, musty or other objectionable odour; and 
c) be free from any extraneous matter, adulterants, harmful consituents, insects or fungus infestation. 
4.2 Specific requirements  
The material shall conform to the requirements specified in Table 1 when tested in accordance with the test 
methods specified therein. 
 
 
 
 
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© EAC 2019  – All rights reserved  3 
 Table 1 — Requirements for maize gluten feed 
 
S/No Characteristic  Requirement  Test method  
Meal  Feed  
I Moisture, per cent by mass, max.  12 12 ISO 6496  
ii Crude fat, per cent by mass, min.a 1 1 ISO 6492  
iii Crud e protein (N x 6.25), per cent by mass, mina 55 20 ISO 5983 -1 
iv Crude fibre, per cent by mass, max.a 3 12 ISO 6865  
v Acid insoluble ash, per cent by mass, max.  0.4 0.4 ISO 5985  
a Characteristic  is on dry matter  basis . 
 
5 Contaminants 
5.1 Aflatoxin  
Aflatoxin contamination in maize gluten shall not be more than 10 ppb for B 1 and 20 ppb for total aflatoxin 
when tested with ISO 16050.  
5.2 Pesticide residues 
Maize gluten shall comply with those maximum limits for pesticide residues stated in Codex Alimentarius 
commission  
5.3 Heavy metal 
Maize gluten shall be free from heavy metals in amounts which may represent a hazard to animals and shall 
comply with the maximum limits of heavy metals as specified in Table 3 when tested in accordance with the 
methods specified therein  
Table 2 — Limits for heavy metals in maize gluten  
S/No Heavy metal  Maximum limit,  
mg/kg Test method  
1 Arsenic  2.0  
ISO 27085  2 Lead  5.0 
3 Cadmium  1.0 
 
6 Hygiene 
Maize gluten shall be prepared in accordance with CAC/RCP 54.  
7 Weights and measures  
Maize gluten shall be weighed according to the weights and measures regulation of the destination country. PUBLIC REVIEW DRAFT 
4 © EAC 2019  – All rights reserved  
 8 Packaging 
Maize gluten feeds for sale shall be packaged in suitable containers that are of sufficient strength, and 
sufficiently sealed to withstand reasonable handling without tearing, bursting or falling open. The containers 
shall be clean and not previously used . 
9 Labelling  
In addition to the requirements in EAS 38, each package shall be legibly and indelibly labelled with the 
following:  
a) expiry date; 
b) name of the feed for example  ― Maize gluten ‖; 
c) name and physical address  of the manufacturer; 
d) net weight in metric units; 
e) batch number /lot identification; 
f) manufacturing date; and 
g) storage instructions. 
10 Sampling 
Sampling shall be done in accordance ISO 6497.  PUBLIC REVIEW DRAFT 
© EAC 2019  – All rights reserved  5 
 Bibliography 
EAS 232:2001, Maize gluten animal feeds specification  
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© EAC 2019  – All rights reserved