ELI: http://data.europa.eu/eli/reg_impl/2023/2646/oj 1/3COMMISSION IMPLEMENTING REGUL ATION (EU) 2023/2646 
of 28 November 2023
concer ning the author isation of a preparation of Lentilactobacillus buchner i DSM 32650 as a feed 
additiv e for all animal species 
(Text with EEA relevance) 
THE EUR OPEAN COMMISSION,
Havi ng regar d to the Treaty on the Functioning of the European Union,
Havi ng rega rd to Regulation (EC) No 1831/2003 of the European Parliament and of the Council of 22 Sept ember 2003 on 
additives for use in animal nutr ition (1), and in particular Article 9(2) thereof,
Whereas:
(1) Regulation (EC) No 1831/2003 provid es for the author isation of additives for use in animal nutr ition and for the 
grounds and procedures for granting such an author isation.
(2) In accordance with Article 7 of Regulation (EC) No 1831/2003, an application was submitted for the author isation 
of a preparation of Lentilactobacillus buchner i DSM 32650. That application was accompanied by the particulars and 
documents required under Article 7(3) of Regulation (EC) No 1831/2003.
(3) The application concer ns the author isation of a preparation of Lentilactobacillus buchner i DSM 32650 as a feed 
additive for all animal species, requesting that additive to be classif ied in the catego ry ‘technological additives ’ and in 
the functional group ‘silag e additives ’.
(4) The European Food Safety Auth ority (‘the Author ity’) concluded in its opinion of 12 May 2023 (2)that, under the 
proposed conditions of use, the preparation of Lentilactobacillus buchner i DSM 32650 is safe for the target species, 
consumers and the envi ronment. In the absence of data, no conclusion could be drawn on the potential of the 
additive to be a skin and eye irritant or a skin sensitiser . Given the proteinaceous nature of the active agent, the 
additive should be considered a respirato ry sensitiser . The Author ity further concluded that Lentilactobacillus buchner i 
DSM 32650 at the proposed inclusion rate may extend the aerobic stability of silage prepared from easy and 
moderately difficult to ensile forag e mater ial with a dry matter range of 28–45 %. The Authority also verified the 
repor t on the method of analysis of the feed additive in feed submitted by the Reference Laborator y set up by 
Regulation (EC) No 1831/2003.
(5) In view of the above, the Commission considers that the preparation of Lentilactobacillus buchner i DSM 32650 
satisfies the conditions provided for in Article 5 of Regulation (EC) No 1831/2003. According ly, the use of that 
preparation should be author ised. In addition, the Commission considers that appropr iate prote ctive measures 
should be taken to prevent adverse effects on the health of the users of the additive.
(6) The measures provid ed for in this Regulation are in accordance with the opinion of the Standing Committ ee on 
Plants, Animals, Food and Feed,
HAS ADOPTED THIS REGUL ATION:
Article 1
The preparation specifi ed in the Annex, belonging to the additive catego ry ‘techn ological additives’ and to the functional 
group ‘silag e additives’, is author ised as an additive in animal nutr ition, subject to the conditions laid down in that Annex.
(1)OJ L 268, 18.10.2003, p. 29.
(2)EFSA Journal 2023;21(6):8055.
Offi cial Jour nal  
of the European Union EN 
L series    
2023/2646  29.11.2023ELI: http://data.europa.eu/eli/reg_impl/2023/2646/oj 2/3Article 2
This Regulation shall enter into force on the twentieth day followi ng that of its publication in the Official Journal of the 
European Union.
This Regulation shall be binding in its entirety and directly applicable in all Member States.
Done at Brussels, 28 November 2023 .
For the Commission
The President
Ursula VON DER LEYENOJ L, 29.11.2023 EN  ELI: http://data.europa.eu/eli/reg_impl/2023/2646/oj 3/3ANNEX 
Identification 
number of 
the additiveAdditiveComposition, chemical formula, descr iption, 
analytical methodSpecies or 
categor y of 
animalMaximum 
ageMinimum 
contentMaximum 
content
Other provisionsEnd of period of 
author isationCFU/kg of fresh 
material
Categor y: technological additiv es. Functional group: silage additiv es
1k21902 Lentilactobacillus 
buchner i DSM 32650Additiv e composition
Preparation of Lentilactobacillus buchner i 
DSM 32650 containing a minimum of 1 × 
1011 CFU/g additive
Solid formAll animal 
species— — 1. In the directions for use of the ad­
ditive and premixtures, the storage 
conditions shall be indicate d.
2. Minimum dose of the additive 
when used without combination 
with other micro-org anisms as si­
lage additives: 1 × 108 CFU/kg of 
easy and moderately difficult to 
ensile fresh mate rial(2).
3. For users of the additive and pre­
mixtures, feed business operat ors 
shall establish operational proce ­
dures and organisational mea ­
sures to address poten tial risks re­
sulting from their use. Where 
those risks cannot be eliminated 
by such procedures and measures, 
the additive and premixtures shall 
be used with personal skin, eye 
and breathing protect ive equip ­
ment.19 December 2033
Character isation of the activ e 
subst ance
Viable cells of Lentilactobacillus buchner i 
DSM 32650
Analytical method (1)
Enumeration in the feed additive of 
Lentilactobacillus buchner i DSM 32650:
— Spread plate (or pour plate) method on 
MRS Agar (EN 15787)
Identif ication of Lentilactobacillus buchner i 
DSM 32650:
— Enterobacter ial Repetitive Inter geni c 
Consensus – Polymerase Chain Reac ­
tions (ERIC-PCR) or DNA sequencing 
methods or Pulsed-Field Gel Electro ­
phoresis (PFGE) – CEN/TS 17697
(1)Details of the analytical methods are available at the following address of the Reference Laborato ry: https://joint-research-c entre.ec.europa.eu/eurl-fa-eurl-f eed-additives/eurl-fa-author isation/eurl-f a-evaluation- 
repor ts_en .
(2)Easy to ensile forage: > 3 % soluble carbohydrat es in fresh material ; moderat ely difficult to ensile forage: 1,5–3,0 % soluble carbohy drates in the fresh mat erial in accordance with Commission Regulation (EC) 
No 429/2008 of 25 Apr il 2008 on detailed rules for the impl ementation of Regulation (EC) No 1831/2003 of the European Parliament and of the Council as regards the preparation and the presentation of 
applications and the assessment and the author isation of feed additives (OJ L 133, 22.5.2008, p. 1).OJ L, 29.11.2023 EN