COMMISSION IMPLEMENTING REGUL ATION (EU) 2022/1382 
of 8 August 2022
concer ning the author isation of a preparation of Prop ionibacter ium freudenr eich ii DSM 33189 and 
Lentilactobacillus buch neri DSM 12856 as a feed additiv e for all animal species 
(Text with EEA relevance) 
THE EUR OPEAN COMMISSION,
Havi ng regar d to the Treaty on the Functioning of the European Union,
Havi ng rega rd to Regulation (EC) No 1831/2003 of the European Parliament and of the Council of 22 Sept ember 2003 on 
additives for use in animal nutr ition (1), and in particular Article 9(2) thereof,
Whereas:
(1) Regulation (EC) No 1831/2003 provid es for the author isation of additives for use in animal nutr ition and for the 
grounds and procedures for granting such an author isation.
(2) In accordance with Article 7(1) of Regulation (EC) No 1831/2003, an application was submitte d for the 
author isation of a preparation of Propionibacter ium freudenreichii DSM 33189 and Lentilactobacillus buchner i DSM 
12856. That application was accompan ied by the particulars and documents required under Article 7(3) of 
Regulation (EC) No 1831/2003.
(3) The application concer ns the author isation of the preparation of Propionibacteriu m freudenreichii DSM 33189 and 
Lentilactobacillus buchner i DSM 12856 as a feed additive for all animal species, to be classified in the additive catego ry 
‘technological additives’.
(4) The European Food Safety Author ity (‘the Author ity’) concluded in its opinion of 26 Januar y 2022 (2)that, under the 
proposed conditions of use, the preparation of Propionibacteriu m freudenreichii DSM 33189 and Lentilactobacillus 
buchner i DSM 12856 does not have an adverse effect on animal health, consumer safety or the environment. It also 
concluded that the additive is not irritant for eyes and skin but should be considered a respirator y sensitiser and no 
conclusions could be drawn on the skin sensitisation poten tial of the additive. Theref ore, the Commission considers 
that appropr iate prot ective measures should be taken to prevent adverse effects on human health, in particular as 
regards the users of the additive. The Author ity also concluded that the preparation concer ned has the potential to 
imp rove the preser vation of nutr ients in silage prepared with easy and moderately difficult to ensile mat erial. It also 
verified the repor t on the method of analysis of the feed additives in feed submitte d by the Reference Laboratory set 
up by Regulation (EC) No 1831/2003.
(5) The assessment of the preparation of Propionibacteriu m freuden reichii DSM 33189 and Lentilactobacillus buchner i DSM 
12856 shows that the conditions for author isation, as provided for in Article 5 of Regulation (EC) No 1831/2003, 
are satisfied. According ly, the use of the preparation should be author ised as specified in the Annex to this 
Regulation.
(6) The measures provid ed for in this Regulation are in accordance with the opinion of the Standing Committ ee on 
Plants, Animals, Food and Feed,
(1)OJ L 268, 18.10.2003, p. 29.
(2)EFSA Jour nal 2022;20(2):7151.EN Offi cial Jour nal of the European Union L 207/16 9.8.2022  HAS ADOPTED THIS REGUL ATION:
Article 1
The preparation specifi ed in the Annex, belonging to the additive catego ry ‘techn ological additives’ and to the functional 
group ‘silag e additives’, is author ised as an additive in animal nutr ition, subject to the conditions laid down in that Annex.
Article 2
This Regulation shall enter into force on the twentieth day followi ng that of its publication in the Official Journal of the 
European Union.
This Regulation shall be binding in its entirety and directly applicable in all Member States.
Done at Brussels, 8 August 2022 .
For the Commission
The President
Ursula VON DER LEYENEN Offi cial Jour nal of the European Union 9.8.2022 L 207/17  ANNEX 
Identifica -
tion 
number of 
the 
additiveAdditiveCompo sition, chemical formula, descr iption, analytical 
methodSpecies or 
categ ory of 
animalMaxi -
mum 
ageMini -
mum 
conten tMaxi -
mum 
conten tOther provisi onsEnd of period of 
author isation
CFU of additive/kg 
of fresh material
Categor y: technological additiv es
Functional group: silage additiv es
1k1801 Propionibacter ium 
freuden reichii DSM 
33189 and 
Lentilactobacillus 
buchner i DSM 
12856Additive composition
Preparation of Propionibacteriu m freudenreichii DSM 
33189 and Lentilactobacillus buchner i DSM 12856 
containing a minimum of 5 × 1011 CFU/g additive, with 
a ratio of 1:4 (1×1011 CFU P. freuden reichii DSM 33189/g 
and 4×1011 CFU L. buchner i DSM 12856/g)
Solid form
Char acter isation of the active substance
Viable cells of Propionibacter ium freudenr eichii DSM 
33189 and Lentilactobacillus buchner i DSM 12856
Analytical method (1)
For the identification of Propionibacteriu m freudenreichii 
DSM 33189 and Lentilactobacillus buchner i DSM 12856:
— Pulsed-Field Gel Electrophoresis (PFGE) or DNA 
sequencing methods
For the enumeration of Lentilactobacillus buchner i DSM 
12856 in the feed additive:
— Spread plate method on MRS agar (EN 15787)
For the enumeration of Propionibacteriu m freudenreichii 
DSM 33189 in the feed additive:
— Pour plate method on caseine peptone, yeast extract, 
sodium lactat e and L-cyst eine agarAll animal 
species- - - 1.In the directions for use of the additive 
and premixtures, the stora ge condi ­
tions shall be indicated .
2.Minimum cont ent of the additive 
when used without combination with 
other micro-org anisms as silage addi ­
tives: 1×108 CFU/kg of easy and mod ­
erately difficult to ensile fresh mater i­
al(2).
3.For users of the additive and premix ­
tures, feed business operat ors shall es­
tablish operational procedures and 
organisational measures to address 
poten tial risks resulting from its use. 
Where those risks cannot be elimi ­
nated or reduced to a minimum by 
such procedures and measures, the 
additive and premixtures shall be used 
with personal protective equipment, 
including skin and breathing prote c­
tion.29 August 
2032
(1)Details of the analytical methods are available at the following address of the Reference Laborato ry: https://joint-research-c entre.ec.europa.eu/eurl-fa-eurl-f eed-additives/eurl-fa-author isation/eurl-f a-evaluation- 
repor ts_en
(2)Easy to ensile forage: > 3 % soluble carboh ydrates in fresh material; moderat ely diffi cult to ensile forag e: 1,5-3,0 % soluble carbohy drates in the fresh mat erial in accordance with Commission Regulation (EC) 
No 429/2008 of 25 Apr il 2008 on detailed rules for the impl ementation of Regulation (EC) No 1831/2003 of the European Parliament and of the Council as regards the preparation and the presentation of 
applications and the assessment and the author isation of feed additives (OJ L 133, 22.5.2008, p. 1).EN Offi cial Jour nal of the European Union L 207/18 9.8.2022