Notice of Modification to the List of 
Permitted Sweeteners  to Enable the 
Use of Steviol Glycosides from Yarrowia  
lipolytica  VRM in a Variety of  Foods  
 
Notice of Modification  – Lists of Permitted Food 
Additives  
Reference Number: NOM/ADM-0169  
July 22, 2021 
  
Notice of Modification to the List of Permitted Sweeteners  to Enable the Use of Steviol Glycosides from Yarrowia  lipolytica  VRM in a 
Variety of  Foods – Reference Number: NOM/ADM -0169| 2 
 Summary  
Food additives are regulated in Canada under Marketing Authorizations  (MAs) issued by the Minister of 
Health and the Food and Drug Regulations  (Regulations) . Approved food additives and their permitted 
conditions of use are set out in the Lists of Permitted Food Additives  that are inco rporated by reference in 
the MAs and published on the Canada.ca website. A petitioner can request that Health Canada approve a 
new additive or a new condition of use for an already approved food additive by filing a food additive 
submission with the Depart ment's Food Directorate. Health Canada uses this premarket approval process to 
determine whether the scientific data support the safety of food additives when used under specified 
conditions in foods sold in Canada.  
Health Canada’s Food Directorate receive d a food additive submission seeking approval for the use of steviol 
glycosides from Yarrowia lipolytica  VRM as a sweetener  in all foods where steviol glycosides1 from other 
sources is currently permitted in Canada and at the same maximum levels of use.  
Steviol glycosides  from Stevia rebaudiana  Bertoni  and Saccharomyces cerevisiae  CD15380 , CD15407 , and 
Y63348  is already permitted for use in Canada as a sweetener  in a variety of standardized and unstandardized  
foods.   
The results of the Food Directorate’s e valuation of available scientific data support the safety of steviol 
glycosides from Y. lipolytica  VRM for the same uses as are already permitted for steviol glycosides from the 
other sources noted above. Therefore, Health Canada has modified the  List of Permitted Sweeteners  to 
enable these uses of steviol glycosides from Y. lipolytica  VRM by adding this source  to Column 1 of Item No. 
S.1.2 of this List, as shown below  in bold . 
 
Modification to the List of Permitted Sweeteners   
Item 
No. Column 1  
Additive  Column 2  
Permitted in or Upon  Column 3  
Maximum Level of Use and 
Other Conditions  
S.1.2  Steviol  glycosides from:    
Stevia 
rebaudiana  Bertoni;   
Saccharomyces 
cerevisiae 
CD15380;  Saccharomyces 
cerevisiae  CD15407 ; 
Saccharomyces 
cerevisiae  Y63348 ; 
Yarrowia lipolytica  VRM  (1) Table -top sweeteners  (1) Good Manufacturing Practice  
… … 
(11) (naming the flavour) Milk; 
(naming the flavour) Partly 
skimmed milk; (naming the 
flavour) Partly skimmed milk 
with added milk solids; (naming 
the flavour) Skim milk; (naming 
the flavour) Skim milk with 
added milk solids  (11) 0.02% (calculated as stevi ol 
equivalents ) 
                                                             
1 When referring to the name of the additive , the term “steviol glycosides” is used in the singular  form . Notice of Modification to the List of Permitted Sweeteners  to Enable the Use of Steviol Glycosides from Yarrowia  lipolytica  VRM in a 
Variety of  Foods – Reference Number: NOM/ADM -0169| 3 
 Rationale  
Health Canada’s Food Directorate completed a premarket safety assessment of steviol glycosides from Y. 
lipolytica  VRM  for use as a sweetener . The assessment conclud ed that information related to allergenicity , 
chemistry, microbiology  and molecular biology , nutrition, and toxicology supports  the safety of steviol 
glycosides from Y. lipolytica  VRM  for its requested uses. Therefore, the Department has enabled the 
requested uses of steviol glycosides from Y. lipolytica  VRM  by modifying  the List of Permitted Sweeteners  as 
shown in the above table.  
Other Relevant Information  
The Food and Drug  Regulations  requi re that food additives , such as steviol glycosides , that do not have food -
grade specifications set out in Part B of the Regulations meet the food -grade specifications set out in the  
most recent  edition of the  Food Chemicals Codex  or the Combined Compendium of Food Additive 
Specifications . The Food Chemicals Codex  is a compendium of standards for purity and identity for food 
ingredients, including food additives, published by the United S tates Pharmacopeial Convention. The 
Combined Compendium of Food Additive Specifications , which contains specifications prepared by the Joint 
FAO/WHO Expert Committee on Food Additives (JECFA), is published by the Food and Agriculture 
Organ ization of the United Nations.  
Implementation and Enforcement  
The above modification came into force July 22, 2021, the day it was published in the List of Permitted 
Sweeteners . 
The Canadian Food Inspection Agency is responsible for the enforcement of the Food and Drugs Act and its 
associated regulations with respect to foods.   
Contact Information  
Health Canada's Food Directorate is committed to reviewing new scientific information on the safety in use of 
any permitted  food additive, including steviol glycosides from Y. lipolytica  VRM . Anyone wishing to submit 
new scient ific information on the use of this additive  or to submit inquiries on this modification may do so in 
writing, by regular mail or electronically. If you wish to contact the Food Directorate electronically, please use 
the words " steviol glycosides (NOM -0169 )" in the subject line of your e -mail.  
Bureau of Chemical Safety, Food Directorate  
251 Sir Frederick Banting Driveway  
Tunney’s Pasture, PL: 2202C  
Ottawa, Ontario K1A 0K9  
E-mail: hc.bcs -bipc.sc@canada.ca