ICS 71.100.70 
 
Reference number  
DRS 489-2: 2022 
© RSB 2022 
  
 RWANDA 
STANDARD  DRS 
489-2  
 
First edition  
2022-mm-dd 
 
Oils for cosmetic  use— Specification  
Part 2: Chia seed oil  
 
   
DRS 489-2: 2022 
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    ©RSB 2021  - All rights reserved  
 In order to match with technological development and to keep continuous progress in industries, 
standards are subject to periodic review. Users shall ascertain that they are in possession of the latest 
editio n 
 
 
 
 
 
 
 
 
© RSB 2021  
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ePortal: www.portal.rsb.gov.rw   DRS 489-2: 2022 
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 Contents  Page 
Foreword  
 
1 Scope  ................................ ................................ ................................ ................................ .............  1 
2 Normative references  ................................ ................................ ................................ ...................  1 
3 Terms and definitions  ................................ ................................ ................................ ..................  2 
4 Requirements  ................................ ................................ ................................ ................................  2 
4.1 General requirements  ................................ ................................ ................................ ..................  2 
 
4.2 Specific requirements  ................................ ................................ ................................ ..................  2 
5 Packag ing ................................ ................................ ................................ ................................ ...... 4 
6 Labelling  ................................ ................................ ................................ ................................ ........  4 
7 Sampling  ................................ ................................ ................................ ................................ ....... 4 
Annex  A (normative)  Det ermination of critical solution temperature  ................................ .................  5 
A.1 General  ................................ ................................ ................................ ................................ ..........  5 
A.2 Procedure  ................................ ................................ ................................ ................................ ...... 5 
 
 
 DRS 489-2: 2022 
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    ©RSB 2021  - All rights reserved  
 Foreword  
Rwanda Standards are prepared by Technical Committees and approved by Rwanda Standards Board (RSB) 
Board of Directors in accordance with the procedures of RSB, in compliance with Annex 3 of the WTO/TBT 
agreement on the preparation, adoption and application of standards.  
The ma in task of technical committees is to prepare national standards. Final Draft Rwanda Standards adopted 
by technical committees are ratified by members of RSB Board of Directors for publication and gazettment as 
Rwanda Standards.  
DRS 489-2 was prepared by T echnical Committee RSB/TC 011, Cosmetics and related products.  
DRS 489 consists of the following parts, under the general title Oils for cosmetic use — Specification : 
— Part 1:  Baobab seed oil  
 
— Part 2:  Chia seeds oil  
 
— Part 3: Passion fruits (maracuja) seed oil  
Committee membership  
The following organizations were represented on the Technical Committee on Cosmetics and related products  
(RSB/TC 011) in the preparation of this standard.  
University of Rwanda -College of Science and Technology (UR -CST)  
Rwanda Food and Drugs Authority (Rwanda -FDA)  
Rwanda Inspectorate, Competition and Consumer protection Authority (RICA)  
Rwanda Forensic Laboratory (RFL)  
Standards for Sustainability (SFS)  
SULFO Industries Rwanda  
ORIBUT Company Ltd  
Divine Hope Company Ltd 
Better Home Ltd  
Beauty Makers Association (BMA)  DRS 489-2: 2022 
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    ©RSB 2021  - All rights reserved  
 Rwanda Standards Board (RSB) – Secretariat  
 
 
 
 
 
 
 
 
Introduction  
Salvia hispanica  L., whose common name is chia, is an annual herbaceous plant that belongs to the Lamiaceae 
family, which is native to southern Mexico and northern Guatemala. It has been cultivated from tropical to 
subtropical regions. It is a possible new crop for food i ndustry. This plant was used by the Aztec tribes in the 
early history of Mesoamerica . 
Chia seeds ( Salvia hispanica L .) belongs to mint family ( Lami -aceae ). Chia plant produces tiny seeds (1mm in 
diameter) which are oval in shape and come in white, grey and  black colours.  
Chia oil is a centuries -old ingredient that has been rediscovered for today cosmetics and nutritional applications. 
Since chia seed oil is a natural product, it has a variable chemical composition depending on several factors 
such as the cu ltivation environment and the extraction system . 
 DRS 489-2: 2022 
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    ©RSB 2021  - All rights reserved  
 Oils for cosmetic use  — Specification  — Part 2: Chia seed oil  
1 Scope  
This working draft specifies the requirements, sampling and test methods for chia seed oil for cosmetic use. 
2 Normative references  
The following documents are referred to in the text in such a way that some or all of their content constitutes 
requirements of this document. For dated references, only the edition cited applies. For undated references, the 
latest edition of the referenced document (including any amendments) applies.  
RS EAS 346, Labelling of cosmeti cs — General requirements  
 RS EAS 846, Glossary of terms relating to the cosmetic industry   
RS EAS 847 -2, Cosmetics — Analytical methods — Part 2: Determination of moisture content and volatile 
matter content   
RS EAS 847 -5, Cosmetics — Analytical methods — Part 5: Determination of unsaponifiable matter ,  
RS EAS 847 -6, Cosmetics — Analytical methods — Part 6: Determination of  melting point  
RS EAS 847 -7, Cosmetics — Analytical methods — Part 7: Determination of specific gravity  
RS EAS 847 -9, Cosmetics — Analytical methods — Part 9: Determination of colour  
RS EAS 847 -10, Cosmetics — Analytical methods - Part 10: Determination of acetyl value and hydroxyl value  
RS EAS 847 -12, Cosmetics — Analytical methods - Part 10: Determination  of flash point by Pensky  — Martens 
closed cap tester  
RS EAS 847 -13, Cosmetics — Analytical methods — Part 13: Determination of rancidity  
RS EAS 847 -16, Cosmetics — Analytical me thods — Part 16: Determination of lead, mercury and arsenic 
content                                                                                                                                                                                  RS 
ISO 660,  Animal  and vegetable fats and oils — Determination of acid value and acidity                                            
RS ISO 663, Animal and vegetable fats and oils — Determination of insoluble impurities content                               
RS ISO 3657 , Animal and vegetable fats and oils — Determination of saponification value                                           
RS ISO 3961, Animal and vegetable fats and oils — Determination of iodine value RS ISO 6320 Animal  and 
vegetable fats and oils — Determin ation of refractive index                                                                                                                     DRS 489-2: 2022 
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    ©RSB 2021  - All rights reserved  
 RS ISO 3960 Animal  and   vegetable fats and oils — Determination of peroxide value — Iodometric (visual) 
endpoint determination    
RS 278, Cosmetics — Methods of sampling  
 RS ISO 6887 -1, Microbiology of food and animal feeding stuffs — Preparation of test samples, initial suspension 
and decimal dilutions for microbiological examination — Part 1: General rules for preparation of the initial 
suspensions and decimal dilutions  
3 Terms and defin itions  
For the purposes of this standard, the terms and definitions given i n RS EAS 846  and the following apply.  
3.1 
chia seed oil  
an oil is obtained from the seeds of chia plant (Salvia hispanica  L.) through different extraction methods which 
include cold pressing, solvent extraction, vacuum steam distillation, and supercritical fluids extraction.  
4 Requirements  
4.1 General requirements  
4.1.1  The product shall be insoluble in water but soluble in aromatic hydroca rbon solvents, 
vegetable and mineral oil.  
4.1.2  When  examined visually, the product shall be:  
a) clear;  
b) free from sediments and other foreign matter; and  
c) free from separated water, added colouring and flavouring substances.  
4.1.3  The product shall be free from admixture with other oils.  
4.2 Specific requirements  
4.2.1  The product shall comply with the requirements given in Table 1 when tested in accordance with the 
methods specified therein.  
 DRS 489-2: 2022 
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 Table 1 — Requirements for chia seed s oil for cosmetic industry  
S/N Characteristics  Requir ements  Test method  
i.  Moisture content, % m/m, max  8.95 RS EAS 847 -2 
ii.  Free fatty acid  content    
iii. J
a Colour in a 1” cell on the Lovibond scale, expressed 
as Y + 5R, max. deepness  4.0 RS EAS 847 -9 
iv.  Insoluble impurities, % m/m, max.  0.25 RS ISO 663  
v.  Refractive index at 2 5 C, range  1.476 -1.492 RS ISO 6320  
vi.  Specific gravity at 20 C 0.92 RS EAS 847 -7 
vii.  Saponification value  192-223 RS ISO 3657  
viii.  Iodine value  193-207 RS ISO 3961  
ix.  Peroxide value, mg/kg, max  2.70-17.6 RS ISO 3960  
x.  Acid value, max.  1.28-2.54 RS ISO 660  
xi.  Unsaponifiable matter, % m/m, max  0.838 -1.28 RS EAS 847 -5 
xii.  Melting point, C min.  25 RS EAS 847-6  
xiii. f Flash point C min.  130 RS EAS 847-12 
xiv.  Test for rancidity  Shall be free from rancidity  RS EAS 847 -13 
xv.  Critical solution temperature, 0ºC, max  0 Annex A  
 
4.2.2  The products shall comply with the limits for heavy metal contaminants in accordance with Table 2, 
when tested in accordance with the methods specified therein.  
Table 2⎯  Limits for heavy metal contaminants for baobab seed oil  
S/N Characteristics  Maximum limit, mg/kg  Test method  
i.  Lead  10 
RS EAS 847 -16 ii.  Arsenic  2  
iii.  Mercury  2  
- The total amount of heavy metals as lead, mercury and arsenic, in combination, in the finished product should not exceed 10 
mg/kg.  
- The heavy metals including lead, mercury and arsenic may be as a result of contamination during processing and should no t 
be deliberately added as ingredients.  
 
 
 DRS 489-2: 2022 
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 4.2.3  The products shall also comply with the microbiological limits given in Table 3 when tested in 
accordance with the methods specified therein.  
Table 3 ⎯ Microbiological limits for chia seed oil  
Characteristics  Maximum limit  Test method  
Total viable count for aerobic mesophyllic micro -
organisms per gram.  100 RS ISO 6887 -1 
Pseudomonas aeruginosa  Not detectable in 0.5 g  RS ISO 22717  
Staphylococcus aureus  RS ISO 22718  
Candida albicans  RS ISO 18416  
5 Packaging  
The product shall be packaged in suitable well -sealed containers that shall protect the contents and shall not 
cause any contamination or react with the products.  
6 Labelling  
6.1  Labelling shall be done in accordance with  RS EAS 346,  
6.2  The words “Chia seeds oil” and ‘For external use only’ shall be conspicuously marked, either 
printed on the label affixed to the container, or lithographed, or stencilled thereon with indelible ink.  
7 Sampling  
Random samples of the product shall be drawn for test in accordance with RS 278 from the market, factory or 
elsewhere.  DRS 489-2: 2022 
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 Annex  A 
(normative)  
 
Determination of critical solution temperature  
A.1 General  
The reagent shall be prepared by diluting ethyl alcohol or rectified spirit with distilled water till the relative density 
of the mixture at 15.5 ºC is 0.8303 ± 0.0001, when compared with distilled water at the same temperature. 
Denatured alcohol shall not be used for this test.   
A.2 Procedur e  
Mix in a test tube, 1.0 g of the oil, with 4.15 times its mass of the reagent. Upon examination, the solution thus 
obtained shall be perfectly clear at 20ºC and shall remain clear when cooled and maintained for 5 minutes at a 
temperature of 0 ºC.  
 DRS xxx: 2022  
 
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