COMMISSION IMPLEMENTING REGUL ATION (EU) 2021/1688 
of 20 September 2021
amending Implementing Regulation (EU) 2020/1201 as regards the lists of host plants and specif ied 
plants, and the tests for the identif ication of Xylella fastidiosa 
THE EUR OPEAN COMMISSION,
Havi ng regar d to the Treaty on the Functioning of the European Union,
Havi ng regard to Regulation (EU) 2016/2031 of the European Parliament and of the Council of 26 October 2016 on 
prote ctive measures against pests of plants, amending Regulations (EU) No 228/2013, (EU) No 652/2014 and (EU) 
No 1143/2014 of the European Parliament and of the Council and repealing Council Directives 69/464/EEC, 74/647/EEC, 
93/85/EEC, 98/57/EC, 2000/29/EC, 2006/91/EC and 2007/33/EC (1), and in particular Article 28(1) and (2) thereof,
Whereas:
(1) Commission Implementing Regulation (EU) 2020/1201 (2)has been applied since August 2020 with the aim to 
prevent further spread within and introduction into the Union of Xylella fastidiosa (Wells et al.) (‘the specif ied pest’ ).
(2) Implementing Regulation (EU) 2020/1201 contains a provision on official checks at introduction into the Union. 
That provision refers to the official checks to be performed at the point of entr y into the Union or at the place of 
destination established in accordance with Commission Directive 2004/103/EC (3). Within the new framewor k for 
official checks established by Regulation (EU) 2017/625 of the European Parliament and of the Council (4), 
Commission Delegat ed Regulation (EU) 2019/2123 (5)established new rules on official chec ks performed at control 
points other than border control posts. Delegat ed Regulation (EU) 2019/2123 repealed Directive 2004/103/EC with 
effect from 14 December 2020 . It is theref ore appropr iate to replace the reference to that Directive by a referen ce to 
Delegat ed Regulation (EU) 2019/2123.
(3) On 23 June 2021 , the European Food Safety Auth ority updat ed the database of host plants susceptible to Xylella 
fastidiosa (Wells et al.). Annex es I and II to Implementing Regulation (EU) 2020/1201 should theref ore be amended 
according ly.
(4) For reasons of transparency and clarity, it is appropr iate to indicate, for each of the tests liste d in Annex IV to 
Implementing Regulation (EU) 2020/1201, the Digital Object Identifier (DOI) whic h allows direct access to the 
scientific articles descr ibing those tests.
(1)OJ L 317, 23.11.2016, p. 4.
(2)Commission Implementing Regulation (EU) 2020/1201 of 14 August 2020 as regar ds measures to prevent the introduction into and 
the spread within the Union of Xylella fastidiosa (Wells et al.) (OJ L 269, 17.8.2020, p. 2).
(3)Commission Directive 2004/103/EC of 7 October 2004 on identity and plant health chec ks of plants, plant products or other objects, 
listed in Part B of Annex V to Council Directive 2000/29/EC, whic h may be carried out at a place other than the point of entr y into the 
Community or at a place close by and specifying the conditions relate d to these checks (OJ L 313, 12.10.2004, p. 16).
(4)Regulation (EU) 2017/625 of the European Parliament and of the Council of 15 March 2017 on official controls and other official 
activities perfor med to ensure the application of food and feed law, rules on animal health and welfare, plant health and plant 
prot ection products, amending Regulations (EC) No 999/2001, (EC) No 396/2005, (EC) No 1069/2009, (EC) No 1107/2009, (EU) 
No 1151/2012, (EU) No 652/2014, (EU) 2016/429 and (EU) 2016/2031 of the European Parliament and of the Council, Council 
Regulations (EC) No 1/2005 and (EC) No 1099/2009 and Council Directives 98/58/EC, 1999/74/EC, 2007/43/EC, 2008/119/EC 
and 2008/120/EC, and repealing Regulations (EC) No 854/2004 and (EC) No 882/2004 of the European Parliament and of the 
Council, Council Directives 89/608/EEC, 89/662/EEC, 90/425/EEC, 91/496/EEC, 96/23/EC, 96/93/EC and 97/78/EC and Council 
Decision 92/438/EEC (Official Controls Regulation) (OJ L 95, 7.4.2017, p. 1).
(5)Commission Delegat ed Regulation (EU) 2019/2123 of 10 October 2019 supplementing Regulation (EU) 2017/625 of the European 
Parliament and of the Council as regar ds rules for the cases where and the conditions under which identity checks and physical chec ks 
on certain goods may be perfor med at control points and documentar y chec ks may be perf ormed at distance from border control 
posts (OJ L 321, 12.12.2019, p. 64).EN Offi cial Jour nal of the European Union L 332/6 21.9.2021  (5) Implementing Regulation (EU) 2020/1201 should theref ore be amended according ly.
(6) The measures provid ed for in this Regulation are in accordance with the opinion of the Standing Committ ee on 
Plants, Animals, Food, and Feed,
HAS ADOPTED THIS REGUL ATION:
Article 1
Implementing Regulation (EU) 2020/1201 is amended as follows:
(1) in Article 33, paragraph 1 is replaced by the followi ng:
‘1. All consignments of host plants, introduced into the Union from a third countr y, shall be offici ally check ed at the 
border control post of first arrival into the Union or at a control point in the cases and conditions set out in 
Commission Delegat ed Regulation (EU) 2019/2123’;
(2) Annex I is replaced by the text set out in Annex I to this Regulation;
(3) Annex II is replaced by the text set out in Annex II to this Regulation;
(4) Annex IV is replaced by the text set out in Annex III to this Regulation.
Article 2
This Regulation shall enter into force on the twentieth day followi ng that of its publication in the Official Journal of the 
European Union.
This Regulation shall be binding in its entirety and directly applicable in all Member States.
Done at Brussels, 20 Septembe r 2021 .
For the Commission
The President
Ursula VON DER LEYENEN Offi cial Jour nal of the European Union 21.9.2021 L 332/7  ANNEX I 
Annex I to Implementing Regulation (EU) 2020/1201 is replaced by the followi ng:
‘ANNEX I
List of plants kno wn to be susceptible to one or more subspecies of the specif ied pest (‘host plants’) 
Acaci a
Acer
Adenocar pus lainzii
Albizia julibr issin Durazz.
Alnus rhombif olia Nutt.
Amar anthus retroflexus L.
Ambro sia
Ampelopsis arbor ea (L.) Koehne
Ampelopsis brevipedunculata (Maxim.) Trautv .
Ampelopsis cordata Michx.
Anthyllis hermanniae L.
Artemisia
Aspar agus acutif olius L.
Athyr ium filix-f emina
Bacchar is
Brassica
Calicotome spinosa (L.) Link
Calicotome villosa (Poiret) Link
Callicar pa amer icana L.
Callistemon citrinus (Cur tis) Skee ls
Calluna vulgaris (L.) Hull
Carya
Cathar anthus roseus
Celtis occidentalis L.
Cerci s canadensis L.
Cerci s occidentalis Torr.
Cerci s siliquastr um L.
Chamaecr ista fasciculata (Michx. ) Greene
Chenopodium album L.
ChionanthusEN Offi cial Jour nal of the European Union L 332/8 21.9.2021  Chitalpa tashk entensis T. S. Elias & Wisura
Cistus
Citrus
Clematis cirrhosa L.
Coelor achis cylindr ica (Michx.) Nash Coffea
Conium maculatum L.
Convo lvulus cneor um L.
Copro sma repens A.Ri ch.
Coron illa
Cyper us eragrostis Lam.
Cytisus
Digitar ia
Diosp yros kaki L.f.
Diplocy clos palmatus (L.) C.Jeffre y
Dodonaea viscosa (L.) Jacq.
Echium plantagineum
Elaeagnus angustif olia L.
Encelia farinosa A. Gray ex Torr.
Eremo phila maculata (Ker Gawler) F. von Müller .
Erigeron
Erodium moschatum (L.) L'Hér it.
Erysimum hybr ids
Euphorbia chamaesyce L.
Euphorbia terracina L.
Euryops chrysanthemoides (DC.) B.Nord
Euryops pectinatus (L.) Cass.
Fagus crenata Blume
Fallopia japonica (Houtt.) Ronse Decr .
Fatsi a japonica (Thunb.) Decne. & Planch.
Ficus carica L.
Fortunella
Frangula alnus Mill.
Fraxinus
Genista
Ginkgo biloba L.EN Offi cial Jour nal of the European Union 21.9.2021 L 332/9  Gleditsia triacanthos L.
Grevil lea juniper ina Br.
Hebe
Helianthus
Helichr ysum
Heliotro pium europaeum L.
Hemero callis
Hevea brasiliensis (Willd. ex A.Juss.) Müll.Arg.
Hibiscus
Humulus scandens (Lour .) Mer r.
Ilex aquifolium L.
Ilex vomitor ia Sol. ex Aiton
Iva annua L.
Jacar anda mimosifolia D. Don
Juglans
Juniper us ashei J. Buchholz
Koelre uteria bipinnata Franch.
Lagerstroemia
Laur us nobilis L.
Lava ndula
Lava tera cretica
Ligustr um lucidum L.
Liquidambar styraciflua L.
Lonicer a implexa
Lonicer a japonica Thunb.
Lupinus aridorum
Lupinus villlosus
Magnolia grandif lora L.
Mallotus paniculatus (Lam.) Müll.Arg.
Medicago arbor ea L.
Medicago sativa L.
Metros ideros
Mimosa
Modiola caroliniana (L.) G. Don
Mor usEN Offi cial Jour nal of the European Union L 332/10 21.9.2021  Myo porum insulare R. Br.
Myr tus communis L.
Nandina domestica Mur ray
Neptunia lutea (Leaven w.) Benth.
Nerium oleander L.
Olea
Osteosper mum ecklonis DC.
Osteosper mum fruticosum (L.) Norl.
Parthenocissus quinquefolia (L.) Planch.
Paspalum dilatatum Poir.
Pelargonium
Perovskia abrotanoides
Persea amer icana Mill.
Phagnalon saxatile (L.) Cass.
Phillyrea angustifolia L.
Phillyrea latifolia L.
Phlomis fruticosa L.
Phoenix
Pinus taeda L.
Pistacia vera L.
Plantago lanceolata L.
Platanus
Pluchea odor ata (L.) Cass.
Polygala grandif lora
Polygala myrtifolia L.
Prunus
Psidium
Pteridium aquilinum
Pyrus
Quercus
Ratibida columnife ra (Nutt.) Wooton & Standl.
Rhamnus
Rhus
Robinia pseudoacacia L.
RosaEN Offi cial Jour nal of the European Union 21.9.2021 L 332/11  Rosmar inus
Rubus
Ruta chalapensis
Salvia mellifer a Greene
Salvia officinalis
Salvia rosmari nus
Sambucus
Santolina chamaecypar issus L.
Santolina magonica
Sapindus saponar ia L.
Sassafr as
Setar ia magna Griseb.
Solidago fistulosa Mill.
Solidago virgaurea L.
Sorghum halepense (L.) Pers.
Spar tium
Stewartia pseudocamellia
Strelitzia reginae Aiton
Streptocar pus
Symphyo trichum divaricatum (Nutt.) G.L.Nesom
Teucrium capitatum L.
Trifolium repens L.
Ulex
Ulmus
Vaccinium
Vinca
Vitis
Westringia fruticosa (Willd.) Druce
Westringia glabra R.Br .
Xanthium strumar ium L.’EN Offi cial Jour nal of the European Union L 332/12 21.9.2021  ANNEX II 
Annex II to Implementing Regulation (EU) 2020/1201 is replaced by the follo wing:
‘ANNEX II
List of plants kno wn to be susceptible to specif ic subspecies of the specif ied pest (‘specif ied plants’) 
Specif ied plants susceptible to Xylella fastidiosa subspecies fastidiosa
Acer
Ambro sia artemisiifolia L.
Calicotome spinosa (L.) Link
Cerci s occidentalis Torr.
Cistus monspeliensis L.
Citrus sinensis (L.) Osbeck
Coffea
Erysimum
Genista lucida L.
Juglans regia L.
Lupinus aridorum
Magnolia grandif lora L.
Medicago sativa L.
Metros ideros
Mor us
Nerium oleander L.
Pluchea odor ata (L.) Cass.
Polygala myrtifolia L.
Psidium
Prunus
Rhamnus alater nus L.
Salvia rosmari num
Rubus rigidus Sm.
Rubus ursinus Cham. & Schldl.
Ruta chalapensis
Sambucus
Spar tium junceum L.
Streptocar pus
Teucrium capitatum L.
Ulmus amer icana L.
Vaccinium corymbosum
Vinca
Vitis
Specif ied plants susceptible to Xylella fastidiosa subspecies multiplex
Acaci a
Acer griseum (Franc h.) PaxEN Offi cial Jour nal of the European Union 21.9.2021 L 332/13  Acer pseudoplatanus L.
Acer rubrum L.
Adenocar pus lainzii
Alnus rhombif olia Nutt.
Ambro sia
Ampelopsis cordata Michx.
Anthyllis hermanniae L.
Artemisia
Athyr ium filix-f emina
Aspar agus acutif olius L.
Bacchar is halimifolia L.
Calicotome spinosa (L.) Link
Calicotome villosa (Poir.) Link
Callistemon citrinus (Cur tis) Skee ls
Calluna vulgaris (L.) Hull
Carya
Celtis occidentalis L.
Cerci s canadensis L.
Cerci s occidentalis Torr.
Cerci s siliquastr um L.
Chionanthus
Cistus
Clematis cirrhosa L.
Convo lvulus cneor um L.
Copro sma repens A. Rich .
Coron illa
Cytisus
Dodonaea viscosa (L.) Jacq.
Echium plantagineum
Elaeagnus angustif olia L.
Encelia farinosa Gray ex Torr.
Erigeron
Erodium moschatum
Euryops chrysanthemoides (DC.) B.Nord.
Euryops pectinatus (L.) Cass.
Fallopia japonica (Houtt.) Ronse Decr .
Ficus carica L.
Frangula alnus Mill.
Fraxinus
Genista
Ginkgo biloba L.
Gleditsia triacanthos L.
Grevil lea juniper ina Br.EN Offi cial Jour nal of the European Union L 332/14 21.9.2021  Hebe
Helianthus
Helichr ysum
Hibiscus syriacus
Ilex aquifolium L.
Iva annua L.
Koelre uteria bipinnata Franch.
Lagerstroemia
Laur us nobilis L.
Lava ndula
Lava tera cretica
Liquidambar styraciflua L.
Lonicer a
Lupinus aridorum
Lupinus villosus Willd.
Magnolia grandif lora L.
Medicago arbor ea L.
Medicago sativa L.
Metros ideros
Myr tus communis L.
Nerium oleander
Olea
Osteosper mum ecklonis (DC.) Norl.
Pelargonium
Perovskia abrotanoides
Phagnalon saxatile (L.) Cass.
Phillyrea angustifolia L.
Phillyrea latifolia
Phlomis fruticosa L.
Pistacia vera L.
Plantago lanceolata L.
Platanus
Polygala myrtifolia L.
Polygala grandif lora
Prunus
Pteridium aquilinum
Quercus
Ratibida columnife ra (Nutt.) Wooton & Standl.
Rhamnus
Robinia pseudoacacia L.
Rosa
Rosmar inus
RubusEN Offi cial Jour nal of the European Union 21.9.2021 L 332/15  Salvia mellifer a Greene
Salvia officinalis
Salvia rosmari nus
Sambucus
Santolina chamaecypar issus L.
Santolina magonica
Sapindus saponar ia L.
Solidago virgaurea L.
Spar tium
Strelitzia reginae Aiton
Ulex
Ulmus
Vaccinium
Vinca
Westringia fruticosa Guer in.
Xanthium strumar ium L.
Specif ied plants susceptible to Xylella fastidiosa subspecies pauca
Acaci a
Amar anthus retroflexus L.
Aspar agus acutif olius L.
Cathar anthus roseus (L.) G. Don
Chenopodium album L.
Cistus albidus L.
Cistus creticus L.
Citrus
Coffea
Dodonaea viscosa (L.) Jacq.
Eremo phila maculata (Ker Gawler) F. von Müller .
Erigeron
Euphorbia chamaesyce L.
Euphorbia terracina L.
Grevil lea juniper ina Br.
Hebe
Heliotro pium europaeum L.
Hibiscus
Laur us nobilis L.
Lava ndula
Myo porum insulare Br.
Myr tus communis L.
Nerium oleander L.
Olea europaea L.
Osteosper mum fruticosum (L.) Norl.EN Offi cial Jour nal of the European Union L 332/16 21.9.2021  Pelargonium
Phillyrea latifolia L.
Pistacia vera
Polygala myrtifolia L.
Prunus
Rhamnus alater nus L.
Spar tium junceum L.
Ulmus parviflorus
Vinca minor L.
Westringia fruticosa (Willd.) Druce
Westringia glabra Br.’EN Offi cial Jour nal of the European Union 21.9.2021 L 332/17  ANNEX III 
Annex IV to Implementing Regulation (EU) 2020/1201 is replaced by the following:
‘ANNEX IV
Tests for the identif ication of Xylella fastidiosa and its subspecies 
A.Tests for the screening and identif ication of the presence of Xylella fastidiosa
1.Real time PCR based on Harper et al., 2010 (and erratum 2013) (1);
2.Loop-mediat ed isother mal amplification (LAMP) based on primers developed by Harper et al. 2010 (and erratum 
2013) (2);
3.Real time PCR based on Ouyang et al., 2013 (3)
4.Conv entional PCR based on Minsavag e et al. 1994 (4)
B.Molecular tests for the identif ication of the subspecies of Xylella fastidiosa
1.Multi Locus Sequence Typing (MLST) based on Yuan et al., 2010 determining all subspecies (5);
2.PCR based on Hernandez-Mar tinez et al., 2006 determining the subspecies fastidiosa, multiplex and sandyi (6);
3.PCR based on Pooler & Hartung 1995 determining the subspecies pauca (7).
_____________
(1)DOI: 10.1094/PHY TO-06-10-0168.
(2)DOI: 10.1094/PHY TO-06-10-0168.
(3)DOI: 10.1371/jour nal.pone.0081647.
(4)DOI:10.1094/Ph yto-84- 456.
(5)DOI: 10.1094/PHY TO-100-6-0601.
(6)DOI: 10.1094/PD-90-1382.
(7)DOI: 10.1007/BF00294703.’EN Offi cial Jour nal of the European Union L 332/18 21.9.2021