DRAFT UGANDA STANDARD  
  DUS 2296 -3 
First  Edition  
2021 -mm-dd 
 
 
 Reference number  
DUS 2296 -3:2021 
© UNBS 2021   
  
Skin applied mosquito repellents — Specification — Part 3: 
Wipes  
 DUS 2296 -3:2021  
ii © UNBS 2021  – All rights reserved  
 Compliance with this standard does not, of itself confer immunity from legal obligations  
A Uganda Standard does not purport to include all necessary provisions of a contract. Users are 
responsible for its correct application  
© UNBS 2021  
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilised in any 
form or by any means, electronic or mechanical, including photocopying and microfilm, without  prior written 
permission from UNBS.  
Requests for permission to reproduce this document should be addressed to  
The Executive Director  
Uganda National Bureau of Standards  
P.O. Box 6329  
Kampala  
Uganda  
Tel: +256 41 4 333 2 50/1/2/3  
Fax: +256 41 4 286 123  
E-mail:  info@unbs.go.ug   
Web: www.unbs.go.ug  DUS 2296 -3:2021  
© UNBS 2021  – All rights reserved  iii 
 Contents  Page 
Foreword  ................................ ................................ ................................ ................................ .............................  v 
1 Scope  ................................ ................................ ................................ ................................ ......................  1 
2 Normative references  ................................ ................................ ................................ ............................  1 
3 Terms and definitions  ................................ ................................ ................................ ...........................  2 
4.  Active ingredients and synergists  ................................ ................................ ................................ ...... 3 
4.1  Natural repellents  ................................ ................................ ................................ ................................ .. 3 
4.2  Synthetic repellents  ................................ ................................ ................................ ..............................  3 
5 Requirements  ................................ ................................ ................................ ................................ .........  4 
5.1 General requirements  ................................ ................................ ................................ ...........................  4 
5.2 Specific requirements  ................................ ................................ ................................ ...........................  4 
5.3 Microbiological requirements  ................................ ................................ ................................ ..............  5 
5.4 Biological efficacy  ................................ ................................ ................................ ................................ . 5 
6 Packaging  ................................ ................................ ................................ ................................ ...............  5 
7 Labelling  ................................ ................................ ................................ ................................ .................  5 
7.2  Secondary packaging  ................................ ................................ ................................ ...........................  6 
8  Sampling  ................................ ................................ ................................ ................................ ...............  6 
Annex  A (normative)  Determination of DEET content  ................................ ................................ ...................  7 
A.1 General  ................................ ................................ ................................ ................................ ...................  7 
A.2 Apparatus  ................................ ................................ ................................ ................................ ...............  7 
A.3 Preparation of calibration curve  ................................ ................................ ................................ ..........  7 
A.4 Procedure  ................................ ................................ ................................ ................................ ...............  7 
A.5 Calculation  ................................ ................................ ................................ ................................ .............  8 
Annex B  (normative)   Determination of total pyrethrins  ................................ ................................ ..............  9 
B.1  General  ................................ ................................ ................................ ................................ ...................  9 
B.2  Reagents  ................................ ................................ ................................ ................................ ...............  9 
B.3  Apparatus  ................................ ................................ ................................ ................................ ...............  9 
B.4  Operating conditions  ................................ ................................ ................................ ...........................  9 
B.5  Preparation of the standar d ................................ ................................ ................................ ................  9 
B.6  Sample preparation  ................................ ................................ ................................ .............................  10 
B.7  Procedure  ................................ ................................ ................................ ................................ ............  10 
B.8  Calculation of t he % total pyrethrins (active ingredient) ................................ ................................ . 10 
Annex C  (normative)  Determination of absorbency rate  ................................ ................................ ............  11 
C.1  Apparatus  ................................ ................................ ................................ ................................ ............  11 
C.2  Preparation of test specimens  ................................ ................................ ................................ ..........  11 
C.3  Procedure  ................................ ................................ ................................ ................................ ............  11 
C.4  Calculation  ................................ ................................ ................................ ................................ ..........  11 
C.5  Test report  ................................ ................................ ................................ ................................ ............  11 
Annex D  (normative)  Determination of moisture content  ................................ ................................ ..........  13 
D.1  Principle  ................................ ................................ ................................ ................................ ..............  13 
D.2  Apparatus  ................................ ................................ ................................ ................................ .............  13 
D.3  Sample preparation  ................................ ................................ ................................ ............................  13 
D.4  Procedure  ................................ ................................ ................................ ................................ ............  13 
D.5  Calculations  ................................ ................................ ................................ ................................ .........  14 
Annex E  (normative)  Determination of length and width  ................................ ................................ ...........  15 
E.1  Apparatus  ................................ ................................ ................................ ................................ .............  15 
E.2  Procedure  ................................ ................................ ................................ ................................ .............  15 DUS 2296 -3:2021  
iv © UNBS 2021  – All rights reserved  
 E.2.1   Procedure for width  ................................ ................................ ................................ ...........................  15 
E.2.2   Procedure for length  ................................ ................................ ................................ .........................  15 
Bibliography  ................................ ................................ ................................ ................................ .....................  16 
 DUS 2296 -3:2021  
© UNBS 2021  – All rights reserved  v 
 Foreword  
Uganda National Bureau of Standards (UNBS) is a parastatal under the Ministry of Trad e, Industry and 
Cooperatives established under Cap 327 , of the Laws of Uganda , as amended .  UNBS is mandated to co -
ordinate the elaboration of standards and is  
(a) a member of International Organisation for Standardisation (ISO) and  
(b) a contact point for the W HO/FAO Codex Alimentarius Commission on Food Standards, and  
(c) the National Enquiry Point on TBT  Agreement  of the World Trade Organisation (WTO).  
The work of preparing Uganda Standards is carried out through Technical Committees. A Technical 
Committee is est ablished to deliberate on standards in a given field or area and consists of key stakeholders 
including government, academia, consumer groups, private sector and other interested parties.  
Draft Uganda Standards adopted by the Technical Committee are widel y circulated to stakeholders and the 
general public for comments. The committee reviews the comments before recommending the draft standards 
for approval and declaration as Uganda Standards by the National Standards Council.  
The committee responsible for t his document is Technical Committee UNBS /TC 301, Chemistry  
WDU S 2296  consists of the following parts, under the general title Skin applied mosquito repellents — 
Specification:  
 Part 1: Lotions, creams, gels and ointments  
 Part 2: Sprays and roll -ons  
 Part 3 : Wipes  
 Part 4: Bathing soaps  
 Part 5: Bracelets, wristbands and patches   
 Part 6: Jelly  
 
 DRAFT UGANDA S TANDARD  DUS 2296 -3:2021  
 
© UNBS 2021  – All rights reserved  1 
 Skin applied mosquito repellents — Specification — Part 3: 
Wipes  
1 Scope   
This Draft Uganda Standard specifies  the requirements, sampling and methods of test for ski n applied 
mosquito repellents prepared as wipes meant to be applied directly to the skin .  
2 Normative references   
The following referenced documents referred to in the text in such a way that some or all of their content 
constitutes requirements of this d ocument. For dated references, only the edition cited applies. For undated 
references, the latest edition of the referenced document (including any amendments) applies.  
CIPAC 740, Determination of picaridin  
CIPAC 667, Determination of ethyl butylacetamido propionate   
US ISO 1833 -1, Textiles — Quantitative chemical analysis — Part 1: General principles of testing  
US ISO 139, Textiles — Standard atmospher es for conditioning and testing  
US ISO 9073 -1, Test methods for nonwovens —Part1: Determination of mass pe r unit area  
ISO 9073 -18, Test methods for nonwovens — Part 18: Determination of breaking strength and elongation of 
nonwoven materials using the grab tensile test.  
US ISO 3071, Textile materials; Method for determination of pH value of aqueous extracts  
ISO 20743, Textiles — Determination of antibacterial activity of textile products  
US ISO 21149, Cosmetics — Microbiology — Enumeration and detection of aerobic mesophilic bacteria  
US ISO 22717, Cosmetics — Microbiology — Detection of Pseudomonas aerugino sa  
US ISO 22718, Cosmetics — Microbiology — Detection of Staphylococcus aureus  
US ISO 18416, Cosmetics — Microbiology — Detection of Candida albicans  
ISO 21150, Cosmetics — Microbiology — Detection of Escherichia coli  
US EAS 96, Sanitary towels — Speci fication  
US EAS 846: 2017, Glossary of terms relating to the cosmetic industry.  
DUS 2373 -1, Mosquito repellents ― Performance test guidelines ― Part 1: Skin applied repellents  
US ISO 2859 -1, Sampling procedures for inspection by attributes — Part 1: Sampling plans indexed by 
Quality Level (AQL) for Lot -By-Lot Inspection.   
2 © UNBS 2021  – All rights reserved  
 3 Terms and d efinitions   
For the purposes of this document, the terms and definitions given in US EAS 846 and the following apply . 
ISO and IEC maintain terminological databases for use in standardization at the following addresses:  
— ISO Online browsing platform: avai lable at http://www.iso.org/obp  
3.1  
mosquito  
blood -sucking dipterous insect of the family Culicidae. Aedes, Anopheles, Culex, Mansonia, and Stegomyia 
are genera containing most species involved in the transmission of protozoan and other disease -producing 
parasites.  
3.2  
mosquito repellent  
substance applied to skin  which deters mosquito from approaching  or settling .  
3.3  
natural repellents  
repellents that contain , plant -based compounds  
3.4  
synthetic repellents  
conventional repellents containing  chemical c ompounds  manufactured to imitate the natural compounds.  
3.5  
absorbency time  
time taken for a liquid to be dispersed into the non -woven disposable wet wipe  
3.6  
acceptable  
acceptable to the authority administering this standard, or to the parties concl uding the purchase contract, as 
relevant  
 3.7  
length of piece  
distance between the beginning and the end of the sample in the lengthwise or machine direction  
3.8  
overall width of piece  
distance between the outermost edges of the sample measured perpe ndicular to the longitudinal edges  
3.9  
usable width of piece  
width of the fabric excluding any selvedge materials, marks, pin -holes or other non -homogeneous areas of the 
fabric.  
3.10  
non-woven  
non-wovens are structures of textile materials, such as f ibres, continuous filaments, or chopped yarns of any 
nature or origin, that have been formed into webs by any means, and bonded together by any means, 
excluding the interlacing of yarns as in woven fabric, knitted fabric, laces, braided fabric or tufted fa bric 
3.11  
wipe  
piece of soft, wet cloth or paper used for wiping  
 DUS  2296 -3:2021 
© UNBS 2021  – All rights reserved  3 
 3.12  
DEET  
N,N-Diethyl -meta -toluamide or diethyltoluamide  
3.13  
IR3535  
ethyl butylacetylaminopropionate  
3.14  
picaridin  
1-(1-methylpropoxycarbonyl) -2-(2-hydroxyethyl) piperidine or 2 -(2-hydroxyethyl) -1-piperidinecarboxylic acid 1 -
methylpropyl ester  
4.  Active ingredients and synergists  
4.1  Natural repellents  
4.1.1  Active ingredients used in natural repellents shall be plant based compounds which are able to deter 
mosquito es from approach ing or settling . Such shall be essential oils or any other plant extract approved as 
mosquito repellents.  
4.1.2  The manufacturer shall provide adequate data on the repellence of such ingredients.  
4.1.3   The manufacturer shall have adequate data justifyin g the proportion of ingredient(s)  used in the 
product , for which claims are made.  
4.1.4   The essential oils and other plant extracts used in natural repellents shall be, but not limited to:  
a)  Cedarwood oil;  
b)  Tea tree oil;  
c)  Geranium oil;  
d)  Rosemary oil;  
e)  Lemongrass oil;  
f)  Citronella oil;  
g)  Eucalyptus oil; and  
h)  Cinnamon oil.  
i)  Neem oil  
4.1.5  The proportion of single or blended active ingredient (s) in natural repellent shall be set by the 
manufacturer in accordance with specific  standard and records shall be availed.  
 4.1.6  Pyrethrum extracts such as pyrethrins shall be considered in natural repellents. The limits of 
pyrethrins in natural repell ents shall not be less than 0.5 % w/w, when tested in accordance with annex B.  
4.2  Synthetic repellents  
4.2.1  Synthetic repellents shall contain chemical compounds which are able to deter mosquitoes from 
approaching or settling on the surface .  
4 © UNBS 2021  – All rights reserved  
 4.2.2  If a synthetic active ingredient is blended with other active ingredient (s), either natura l or synthetic, 
the proportion shall be set by the manufacturer based on scientific research and records shall be availed.  
4.2.3   Synthetic repellents and their active ingredients shall be approved and registered by competent 
authority before being release d to the market.   
5 Requirements  
5.1 General  requirements  
5.1.1  The product shall constitute a mosquito repellent that is formulated as wipes and shall be essentially a 
product which has active ingredient (s) added to a certain level.  
5.1.2  The product shall be of acceptable uniform make and finish.  
5.1.3  The product shall be free from defects that might impair their appearance or serviceability (or both).  
5.1.4  When applied to the skin, the product shall have the benefit of repelling mosquitoes and shall not 
cause harmful effect to the skin .  
5.2 Specific requirements  
5.2.1  Active ingredients and their content in synthetic repellents shall meet the requirements prescribed in 
table 1.  
Table 1— Active ingredients content for synthetic repellents  in for m of wipes .  
S/N  Characteristic  Requirements  Test methods  
i DEET , % w/w.  4 – 50 Annex A  
ii Picaridin , % w/w.  5 – 20 CIPAC 740  
iii IR3535 , % w/w.  7.5 – 20.07 CIPAC 667  
 
5.2.2 The product shall comply with the specific requirement s given in table 2 when  tested in accord ance  to 
the methods described therein.  
Table 2 – Specific requirements for  skin applied mosquito repellent s in form of wipes . 
S/N  Parameter  Requirements  Test methods  
i Fibre composition  Cellulose , % min  20 US ISO 1833 -1 
Others, % ma x 80 
ii Grammage, g/m2 , . min 36 US ISO 9073 -1 
iii pH 5.5 – 8.0 US ISO 3071  
iv Absorbency time, sec, max (for manufacturer)  5 Annex C 
v Flushability  Pass the test  US EAS 96  
vi Number of wipes, pcs  As declared  Sensory  
vii Moisture content, % , m/m, Min  50 Annex D 
viii Size, mm   Length  As declared on the label  Annex E 
Width  DUS  2296 -3:2021 
© UNBS 2021  – All rights reserved  5 
 ix Breaking 
strength, 
N, min.  Machine 
direction  Dry 60  
ISO 9073 -18 Wet 30 
Cross 
section  Dry 3.5 
Wet 2.5 
x Anti-Bacterial activity (A), min  2 ISO 20743  
 
5.3 Microbiolog ical requirements   
The product shall comply with the microbiology limits specifi ed in table 3 when tested in accordance to the 
methods described therein  
Table 3— Microbiology limits for skin applied mosquito repellent in form of wipes . 
S/No Char acteristic . Requirements  Test methods  
i Total viable count, cfu/g, max  1000 US ISO 21149  
ii Staphylococcus aureus (per g)  Not detected  US ISO 22718  
iii Pseudomonas aeruginosa (per g)  Not detected  US ISO 22717  
iv E. coli  Not detectable  ISO 21150  
v Candida albicans (per g)  Not detected  US ISO 18416  
 
 
5.4 Biological efficacy  
When tested in accordance with DUS 2373 -1, the product shall repel 100 % of the mosquitoes from 
approaching or settling on that surface, within protection time indicated by the man ufacturer.  
6 Packaging  
The product shall be packaged in suitable well -sealed containers that shall protect the contents and shall not 
cause any contamination or react with the product.  
7 Labelling  
7.1 The primary packages  shall be labelled with legible an d indelible pre -printed marking bearing the 
following information:  
a)  name of product ‘mosquito repellent wipes”  
b) name  and physical  address of manufacturer ;  
c)  importer/distributors name, address (if applicable);  
d)  number of wipes in a pack;  
e)  intended use, e.g repellent;  
f)  size of wipe in the pack;   
6 © UNBS 2021  – All rights reserved  
 g)  ingredients “fibre content” or “material content” and active ingredient used;  
h)  instruction for use, storage and disposal;  
i)  country of origin;  
j)  date of manufacture  
k) expiry; or be st before  date;  
l)  batch number;  
m)  safety caution; and  
n)  special population whose exposure is prohibited (children and pregnant women)  
o) protection time  
7.2  Secondary packaging  
The outside of each secondary pack shall bear the following informat ion in legible and indelible marking:  
a)  the manufacturer’s name and/or registered trade mark;  
b)  the words “ non-woven  disposable wet wipes”; and  
c)  the number of packages.  
8  Sampling  
Sampling shall be done  in accordance with US ISO 2859 -1. 
 
  DUS  2296 -3:2021 
© UNBS 2021  – All rights reserved  7 
 Annex A 
(normative)  
 
Determination of DEET content  
A.1 General  
The sample is dissolved in carbon disulfide and the difference in absorbance at 14.18 μm and at 14.48 μm is 
determined. The quantity of meta -isomer is obtained from this value by means of a calibr ation curve prepared 
by the use of a reference standard  
A.2 Apparatus  
A.2.1  Double -beam infrared spectrophotometer. Perkin -Elmer model 21 or equivalent  
A.2.2  Two equivalent infrared absorption cells, with sodium chloride windows and a path length of 
approx imately 0.4 mm.  
A.3 Preparation of calibration curve  
A.3.1  Weigh (to the nearest 0.1 mg) into four volumetric flasks sufficient amounts of the reference DEET 
standard of known purity to give concentrations of approximately 20, 40, 60 and 80 g/L when dissol ved in 
carbon disulfide . 
A.3.2  Fill the reference cell with carbon disulfide and the sample cell with each of the standard solutions in 
turn, and record the spectra. The spectrum may be scanned rapidly, except for the region 12 – 15 μm, where 
a normal speed should be used. Carry out a blank measurement with carbon disulfi de to correct for any 
inequality in the paired cells and to determine whether a cell correction is required . 
A.3.3  Measure the absorbance at 14.18 μm and at 14.48 μm and calculate the difference between these 
values, ΔA, for each of the solutions. Plot the  values of ΔA against the concentration (g/L) of the meta -isomer.  
A.3.4  If a cell correction is required, the value of ΔA is determined from the formula:  
ΔA = [A14.18 – A14.48]ref. - [A14.48]blank  
Where ref. = determination with reference standard  
blank = determination on CS2 blank  
A.4 Procedure  
Weigh (to the nearest 0.1 mg)  about 0.5 g of the sample, transfer quantitatively to a 10 mL volumetric flask, 
and make up to the mark with carbon disulfide. Measure the infrared absorption at 14.18 μm and 14.48 μm 
using the same conditions as described in clause  A.3. Determine the concentration of meta -isomer by 
comparing this value with the calibration curve. A standard sample should be run each day to check the 
calibration of the instrument.   
8 © UNBS 2021  – All rights reserved  
 A.5 Calculation  
DEET co ntent (g/kg) =  (𝐶1 𝑥 𝑃)/𝐶2 
where,  
C1 = concentration (g/L) of standard DEET found from calibration curve  
C2 = concentration (g/L) of sample taken  
P = purity (g/kg) of the reference standard  DUS  2296 -3:2021 
© UNBS 2021  – All rights reserved  9 
 Annex B  
(normative)  
 
 Determination of total pyrethrins  
B.1  General  
The ac tive ingredients in pyrethrum extract may be determined using a HPLC system first by injecting a 
solution of the analyte into the chromatograph, followed by the separation and comparison of peaks areas of 
the analytes in the sample with that of an external  standard containing a known amount of the analytes. The 
peaks are eluted in the following order: Cinerin II, Pyrethrin II, Jasmolin II (total Pyrethrins II) and Cinerin I, 
Pyrethrin I, Jasmolin I (total Pyrethrins I).  
B.2  Reagents  
B.2.1 World pyrethrum  standard, 50 %  
B.2.2 Acetonitrile, HPLC grade  
B.2.3 Water, HPLC grade  
B.3  Apparatus  
A liquid chromatography  System equipped with an auto -sampler, a Variable Wavelength Detector (or 
equivalent) and a Column {Phenomenex, 250 x 4.6 mm Luna Phenyl -Hexyl 5 μ Reverse Phase (or 
equivalent)}.  
B.4  Operating conditions  
B.4.1 Flow rate: 1.5 ml/min  
B.4.2 Composition: 40:60 (%, v/v water/acetonitrile)  
B.4.3 Elution: Isocratic  
B.4.4 Oven temperature: 40 ºC  
B.4.5 Wavelength: 240 nm  
B.4.6 Injection Volume: 15 µ l  
B.4.7 Stop time: 22 min  
B.4.8 Post time: 1 min  
B.5  Preparation of the standard  
Weigh 20 mg of the pyrethrum standard to the nearest 0.0001 g in a 100 mL volumetric flask and dilute to 
volume with Acetonitrile and label it. Transfer a small portion to  a sample vial and label it accordingly.   
10 © UNBS 2021  – All rights reserved  
 B.6  Sample preparation  
In a 100 ml volumetric flask, weigh 20 mg to the nearest 0.0001 g of the sample to be analyzed and dilute to 
volume with Acetonitrile. Sample this solution using a vial and label it accordi ngly.  
B.7  Procedure  
After the chromatograph is stable, make a minimum of three injections for the standard solution as well as for 
the analyte and average the area counts. The relative Standard Deviation between injections should be within 
2 %. 
B.8  Calc ulation of the % total pyrethrins (active ingredient)  
The % Total Pyrethrins is calculated as follow:  
% 𝑎𝑐𝑡𝑖𝑣𝑒  𝑖𝑛𝑔𝑟𝑒𝑑𝑖𝑒𝑛𝑡𝑠  = (𝐴𝑣𝑒𝑟𝑎𝑔𝑒  𝑠𝑎𝑚𝑝𝑙𝑒  𝑎𝑟𝑒𝑎  𝑋 𝑤𝑒𝑖𝑔ℎ𝑡 𝑜𝑓 𝑠𝑡𝑎𝑛𝑑𝑎𝑟𝑑  𝑋 𝑃𝑢𝑟𝑖𝑡𝑦  𝑜𝑓 𝑡ℎ𝑒 𝑠𝑡𝑎𝑛𝑑𝑎𝑟𝑑  (𝑖𝑛 %)) ÷ 
(𝐴𝑣𝑒𝑟𝑎𝑔𝑒  𝑠𝑡𝑎𝑛𝑑𝑎𝑟𝑑  𝑎𝑟𝑒𝑎 𝑋 𝑊𝑒𝑖𝑔ℎ𝑡 𝑜𝑓 𝑠𝑎𝑚𝑝𝑙𝑒 .) DUS  2296 -3:2021 
© UNBS 2021  – All rights reserved  11 
 Annex C  
(normative)  
 
Determination of absorbency rate  
C.1  Apparatus  
C.1.1  Water tub , of a depth of at least 100 mm and maintained at room temperature.  
C.1.2  Stop watch , with an accuracy of 0.2 s  
C.1.3  Cylindrical basket , weighing 2.7 ± 0.3 g of height 80 mm , diameter 50 mm with square opening of 
15 mm to 20 mm, made of copper wire of 0.4 mm diameter.  
C.1.4  Weighing machine  
C.1.5  Forceps  
C.2  Preparation of test specimens  
Take three test specimens, each of mass at least 1 g and composed of a number of p inches of fibres taken 
from widely separated parts of the conditioned laboratory sample.  
C.3  Procedure  
C.3.1  Compress the first test specimen to a volume of approximately 20 mL.  
C.3.2  By means of the forceps, place the test specimen lightly on the su rface of the distilled water and 
simultaneously start the stopwatch.  
C.3.3  Using the stop watch, measure the time it takes the basket and its contents to sink below the surface 
of water in seconds.  
C.3.4  Record the absorption period to the nearest 0.1 s.  
C.3.5  Repeat the test for at least three test specimens.  
C.4  Calculation  
Calculate, to the nearest second, the arithmetic mean of the three test results.  
C.5  Test report  
Report the following information  
a)  all the data needed to identify the la boratory sample tested;  
b)  confirmation that the test was carried out in accordance with this standard;   
12 © UNBS 2021  – All rights reserved  
 c)  any deviation from this standard; and  
d)  the mean absorbency rate, expressed in seconds, of the absorbent cotton wool  
 DUS  2296 -3:2021 
© UNBS 2021  – All rights reserved  13 
 Annex D  
(normative)  
 
Determination of moisture content  
D.1  Principle  
A specimen of specified mass of filler material of the non -woven disposable wet wipe is dried in an oven at 
specified temperature and the moisture content is determined.  
D.2  Apparatus  
D.2.1  Balance , with an accuracy of 0.05% of the weighed mass  
D.2.2  Sample container , waterproof when sealed, will be used for transfer of analyzed material and during 
weighing  
D.2.3  Oven , well ventilated with a temperature of 102 ºC to 105 ºC  
D.3  Sample preparation  
D.3.1  Take a sufficient number of dry sample containers, number them and take their masses after they are 
held open for a short period of time so that they will have the same air pressure as the surrounding 
atmosphere. Then leave them open until you take the  test piece.  
D.3.2  Take 5 random pieces of the wet wipe. The test pieces shall weigh 5 g.  
D.3.3  If the surrounding atmosphere is hot and humid, prevent water condensation on the internal and 
external surfaces of the container.  
D.3.4  Handle the test p ieces gently to prevent dirt or changes in water content. Don’t touch the test pieces 
with your bare hands. Put the test pieces in a container just after taking them and close the container 
immediately.  
D.4  Procedure  
D.4.1  Dry the test pieces in an ove n with a temperature of 102ºC to 1050C. Open the containers lid and dry 
the specimen inside the container. Open the container for a moment, to balance the air pressure inside the 
container with the surrounding pressure, weigh the container that holds the s pecimen again and calculate the 
weight of the specimen.  
D.4.2  First cycle of drying will last at least 30 minutes. Return the container with the test pieces to the oven, 
for at least half the first cycles drying time. Take the container out and take the mass with the test pieces 
inside. Repeat the drying and weighing cycles. When the drying time on every cycle is at least half of the total 
previous drying cycle times. Continue the process until the difference between two consecutive masses does 
not exceed  0.1 % of the original mass of the specimen.   
14 © UNBS 2021  – All rights reserved  
 D.5  Calculations  
Calculate the moisture content using the following formula and round the results up to the nearest 0.1 %.  
𝑉 = 100((𝑎−𝑏) ÷ 𝑐) 
 
Where,  
a is weight of the container with the specimen before  drying (in grams);  
b is weight of the container with the specimen after drying (in grams);  
c is weight of the container (in grams); and  
V  is water content (in weight %).  DUS  2296 -3:2021 
© UNBS 2021  – All rights reserved  15 
 Annex E  
(normative)  
 
Determination of length and width  
E.1  Apparatus  
E.1.1  Steel scale  that is of a length exceeding the width of the fabric to be measured, and is graduated in 
centimeters and millimeters.  
E.1.2  Marking pen .  
E.2  Procedure  
E.2.1  Procedure for width  
E.2.1.1   Lay the test sample flat and full width (without su bjecting it to tension) on a plane surface and 
condition it in that state for at least 24 hrs in accordance with US ISO 139 .  
E.2.1.2   Take, to the nearest 1 mm, five measurements across the overall width or between the innermost 
selvedge threads (as relev ant) of the conditioned test sample at approximately equal intervals throughout its 
length.  
E.2.1.3  Calculation  
Calculate the arithmetic mean of the five measurements and record it as the width of the sample.  
E.2.2   Procedure for length  
E.2.2.1   Take a laboratory sample as specified in the relevant product specification. Where no 
specification exists, take the laboratory sample as agreed upon between the test laboratory and the 
manufacturer to ensure a reasonable and acceptable reliability at a reasona ble and acceptable confidence 
level.  
E.2.2.2   Lay the laboratory sample flat and full width (without subjecting it to tension) on a plane surface 
and condition it in that state for at least 24 hrs in accordance with  US ISO 139 .  
E.2.2.3   From the conditio ned laboratory sample cut a test specimen across the full width of the laboratory 
sample along a datum line drawn at right angles to the selvedges and as close as possible to the beginning 
and the end of the laboratory sample.  
E.2.2.4   Take, to the neares t millimeter, five measurements ( see E.2.1.2)  of the length of the test 
specimen at approximately equal intervals across its width.  
E.2.2.5   Calculation  
Calculate the arithmetic mean of the five measurements and record it as the length, in metres (accura te to the 
nearest centimeter), of the laboratory sample.  
  
16 © UNBS 2021  – All rights reserved  
 Bibliography  
[1] DRS 392 -3: 2018, Skin applied mosquito repellents — Specification — Part 3: Wipes  
[2] RS 191: 2019, Refined pyrethrum concentrate — Specification  
 
  DUS  2296 -3: 202 1 
© UNBS 2021  – All rights reserved  17 
 Certification marking  
Products that conform to Uganda standards may be marked with Uganda National Bureau of Standards 
(UNBS) Certification Mark shown in the figure below.  
The use of the UNBS Certification Mark is governed by the Standards Act, and the Regulations made 
thereunder. This  mark can be used only by those licensed under the certification mark scheme operated by 
the Uganda National Bureau of Standards and in conjunction with the relevant Uganda Standard. The 
presence of this mark on a product or in relation to a product is an assurance that the goods comply with the 
requirements of that standard under a system of supervision, control and testing in accordance with the 
certification mark scheme of the Uganda National Bureau of Standards. UNBS marked products are 
continually chec ked by UNBS for conformity to that standard.  
Further particulars of the terms and conditions of licensing may be obtained from the Director, Uganda 
National Bureau of Standards.  
 
   DUS 2296 -3: 2021  
 
ICS 71.100.70  
Price based on nn pages  
© UNBS 2021 – All rights reserved