Notice of Modification to the List of 
Permitted Food Enzymes  to Enable the 
Use of Lysophospholipase from 
Trichoderma  reesei RF7206  in the 
Production of  Sugars and Dextrins from 
Starch   
Notice of Modification  – Lists of Permitted Food 
Additives  
Reference Number: NOM/ADM-0172 
August 10, 2021 
 Notice of Modification to the List of Permitted Food Enzymes  to Enable the Use of Lysophospholipase from Trichoderma  reesei 
RF7206 in the Production of Sugars and Dextrins from Starch  – Reference Number: NOM/ADM -0172| 2 
 Summary  
Food additives are regulated in Canada under Marketing Authorizations  (MAs) issued by the Minister of 
Health and the Food and Drug Regulations  (Regulations) .  Approved food additives and their permitted 
conditions of use are set out in the Lists of Pe rmitted Food Additives  that are incorporated by reference in 
the MAs and published on the Canada.ca website. A petitioner can request that Health Canada approve a 
new additive or a new condition of use for an already approved food additive by filing a food  additive 
submission with the Department's Food Directorate. Health Canada uses this premarket approval process to 
determine whether the scientific data support the safety of food additives when used under specified 
conditions in foods sold in Canada.  
Heal th Canada’s Food Directorate received a food additive submission seeking approval for the use of  
lysophospholipase from Trichoderma  reesei  RF7206  in starch used in the production of dextrins, dextrose, 
fructose syrups and solids, glucose (glucose syrup), glucose solids (dried glucose syrup), or maltose . The food 
enzyme is intended to be used at a level consistent with Good Manufacturing Practice1. Its purpose of use is 
to improve the efficiency of  the starch process ing.  
Lysophospholipase is a type of phos pholipase . Phospholipase from other microbial sources is already 
permitted for use in Canada as a food enzyme in certain foods , but not in the production  of sugars and 
dextrins from starch.  In addition, T. reesei  RF7206 was not already a permitted source for any  food  enzyme in 
Canada.  
The results of the Food Directorate’s evaluation of available scientific data support the safety and efficacy of 
lysophospholipase from T. reesei  RF7206  when used as  requested by the petitioner . Therefore, Health 
Canada has m odified the List of Pe rmitted Food Enzymes  to extend the use of lysophospholipase from T. 
reesei  RF7206  by adding the e ntry shown below to the list.  
 
Modification to the List of Permitted Food Enzymes  
Item 
No. Column 1  
Additive  Column 2  
Permitted 
Source  Column 3  
Permitted in or Upon  Column 4  
Maximum Level of Use 
and Other Conditions  
P.5.2  (i) 
Lysophospholipase  Trichoderma  
reesei  RF7206  Starch used in the 
production of dextrins, 
dextrose, fructose syrups 
and solids, glucose 
(glucose syrup ), glucose 
solids (dried glucose 
syrup) or maltose  Good Manufacturing 
Practice  
                                                             
1 See the definition of G ood Manufacturing Practice  in the Marketing Authorization for Food Additives That May Be 
Used as Food Enzymes . 
 Notice of Modification to the List of Permitted Food Enzymes  to Enable the Use of Lysophospholipase from Trichoderma  reesei 
RF7206 in the Production of Sugars and Dextrins from Starch  – Reference Number: NOM/ADM -0172| 3 
 Rationale  
Health Canada’s Food Directorate completed a premarket safety and efficacy  assessment of 
lysophospholipase from T. reesei  RF7206 for use as a food enzyme . The Department concluded that 
information related to allergenicity , chemistry, microbiology  and molecular biology, nutrition  and toxicology  
supports the safety of lysophospholipase from T. reesei  RF7206  for its requested use , and technical 
information indicate  that the enzyme is effective for its intended purpose . Therefore, the Department has 
enabled the requested use of lysophospholipase from T. reesei  RF7206 by adding to the List of Permitted 
Food Enzymes  the new entry shown in the above table.  
Other Relevant Information  
The Food and Drug Regulations  require that food additives , such as lysophospholipase , that do not have 
food -grade specifications set out in Part B of the Regulations meet the food -grade specifications set out in 
the most recent  edition of the  Food Chemicals Codex  or the Combined Compendium of Food Additive 
Specifications . The Food Chemicals Codex  is a compe ndium of standards for purity and identity for food 
ingredients, including food additives, published by the United States Pharmacopeial Convention.  
Specifications in the Combined Compendium of Food Additive Specifications  and its associated General 
Specifi cations and Considerations for Enzyme Preparations  are prepared by the Joint FAO/WHO Expert 
Committee on Food Additives (JECFA) , both of which are  published by the Food and Agriculture Organization 
of the United Nations.  
Implementation and Enforcement  
The above modification came into force August 10, 2021, the day it was published in the List of Permitted 
Food Enzymes . 
The Canadian Food Inspection Agency is responsible for the enforcement of the Food and Drugs Act and its 
associated regulations with respect to foods.   
Contact Information  
Health Canada's Food Directorate is committed to reviewing new scientific informati on on the safety in use of 
any permitted  food additive, including lysophospholipase from T. reesei RF7206 . Anyone wishing to submit 
new scientific information on the use of this additive  or to submit inquiries on this modification may do so in 
writing, by regular mail or electronically. If you wish to contact the Food Directorate electronically, please use 
the words " Lysophospholipase  (NOM -0172)" in the subject line of your e -mail.  
Bureau of Chemical Safety, Food Directorate  
251 Sir Frederick Banting Driveway  
Tunney’s Pasture, PL: 2202C  
Ottawa, Ontario K1A 0K9  
E-mail: hc.bcs -bipc.sc@canada.ca