DEAS 231 :2019  
ICS 65.120   
 
© EAC 2019                                                                                                                               2nd Edition 2019   
 DRAFT EAST AFRICAN STANDARD  
Bone meal for  animal feeds — Specification   
EAST AFRICAN COMMUNITY  
 DEAS 231 : 2019  
ii © EAC 2019  – All rights reserved  
 Copyright notice  
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 DEAS 231 : 2019  
© EAC 2019  – All rights reserved  iii 
 Foreword  
Development of the East African Standards h as been necessitated by the need for harmonizing requirements 
governing quality of products and services in the East African Community. It is envisaged that through 
harmonized standardization, trade barriers that are encountered when goods and services are  exchanged 
within the Community will be removed.  
The Community has established an East African Standards Committee  (EASC)  mandated to develop and 
issue East African Standards  (EAS) . The Committee is composed of representatives of the National 
Standards Bo dies in Partner States, together with the representatives from the public and private sector 
organizations  in the community .  
East African Standards are developed through  Technical Committees that are representative of key 
stakeholders including government , academia, consumer groups, private sector and other interested parties. 
Draft East African Standards are circulated to stakeholders through the National Standards Bodies in the 
Partner States. The comments received are discussed and incorporated before f inalization of standards, in 
accordance with the Principles and procedures for development of East African Standards.  
East African Standards are subject to review, to keep pace with technological advances. Users of the East 
African Standards are therefore expected to ensure that they always have the latest versions of the standards 
they are implementing .  
The committee responsible  for this document is Technical Committee EASC /TC 001, Animal feeds and 
feeding stuffs . 
Attention is drawn to the possibility tha t some of the elements of this document may be subject of patent 
rights. EAC shall not be held responsible for identifying any or all such patent rights.  
This second  edition cancels and replaces the first edition ( EAS 231:2001), which has been technically r evised.  
 DRAFT EAST AFRICAN STANDARD  DEAS 231 :2019  
 
© EAC 2019  – All rights reserved  1 
 Bone meal for animal feeds  — Specification  
1 Scope   
This Draft East African Standard specifies requirements, sampling and test methods for bone meal used as a 
mineral supplement in animal feeds.  
2 Normative references   
The following documents are  referred to in the text in such a way that some or all of their content constitutes 
requirements of this document.  For dated references, only the edition cited applies. For undated references, 
the latest edition of the referenced document (including any a mendments) applies.  
IS0 5510, Animal feeding stuffs — Determination of available lysine  
ISO 5983 -1, Animal feeding stuffs — Determination of nitrogen content and calculation of crude protein 
content — Part 1: Kjeldahl method  
ISO 5984, Animal feeding stuffs  — Determination of crude ash  
ISO 5985, Animal feeding stuffs — Determination of ash insoluble in hydrochloric acid  
ISO 6490 -1, Animal feeding stuffs — Determination of calcium content — Part 1: Titrimetric method  
ISO 6491, Animal feeding stuffs — Determin ation of phosphorus content — Spectrometric method  
ISO 6 492, Animal feeding stuffs — Determination of fat content  
ISO 6495, Animal feeding stuffs — Determination of water -soluble chlorides content  
ISO 6496, Animal feeding stuffs — Determination of moisture  and other volatile matter content  
ISO 6497 , Animal feeding stuffs — Sampling  
ISO 6865, Animal feeding stuffs — Determination of crude fibre content — Method with intermediate filtration  
ISO 9831, Animal feeding stuffs, animal products, and faeces or urine  — Determination of gross calorific value 
— Bomb calorimeter method  
ISO 13903, Animal feeding stuffs — Determination of amino acids content  
ISO 14718, Animal feeding stuffs — Determination of aflatoxin B1 content of mixed feeding stuffs — Method 
using high -performance liquid chromatography  
ISO 17375, Animal feeding stuffs — Determination of aflatoxin B1  
ISO 16050 , Foodstuffs — Determination of aflatoxin B1, and the total content of aflatoxins B1, B2, G1 and G2 
in cereals, nuts and derived products — High-performance liquid chromatographic method   
2 © EAC 2019  – All rights reserved  
 EAS 38, Labelling of pre -packaged foods — General requirements  
ISO 27085, Animal feeding stuff — Determination of calcium, sodium, phosphorous, magnesium, potassium, 
iron, zinc, copper, manganese, cobalt, molybdenum,  arsenic, lead and cadmium by ICP -AES 
ISO 6579 -1, Microbiology of the food chain — Horizontal method for the detection, enumeration and 
serotyping of Salmonella — Part 1: Detection of Salmonella spp  
ISO/TS 17919 , Microbiology of the food chain — Polymerase  chain reaction (PCR) for the detection of food -
borne pathogens — Detection of botulinum type A, B, E and F neurotoxin -producing clostridia  
ISO 21871 , Microbiology of food and animal feeding stuffs — Horizontal method for the determination of low 
numbers o f presumptive Bacillus cereus — Most probable number technique and detection method  
3 Terms and definitions   
For the purposes of this document, the following terms and definitions apply.  
ISO and IEC maintain terminological databases for use in standardiza tion at the following addresses:  
— ISO Online browsing platform: available at http://www.iso.org/obp  
3.1  
bone meal  
product obtained from under composed bones after removal of adhering tissues, defatting, sterilization, drying 
and milling   
3.2  
 extraneo us matter  
organic or inorganic materials other than bone meal  such as sand, stones, chaff, dead insects and others  
3.3  
ruminants  
animals with a stomach divided into four compartments (one of which is called a rumen) and chew a cud 
consisting of regurgitat ed, partially digested food. Ruminants include cattle, buffalo, sheep, goats, deer, 
antelopes and camels.  
3.4  
monogastrics  
also called non -ruminants are animals with a single compartment stomach. They include; poultry, swine , cats 
and horses   
4 Requiremen ts 
4.1 General requirements  
Bone meal shall  be: 
a) free from extraneous  matter ; and 
b) free from any objectionable odour, toxic ingredients, adulterants, insects and pathogenic micro 
organisms .  
© EAC 2019  – All rights reserved  3 
 4.2 Specific requirements   
4.2.1  Bone meal shall conform to th e requ irements in Table 1 when tested in accordance with the test 
methods specified therein  
Table 1 — Specific requirements for bone meal  
 
Parameter  Requirement  
(per cent by mass)  Test m ethod*  
 
Moisture content, max.  7.5 ISO 6496  
Crude fat, max.  3 ISO 6 492 
Calciu m, min.  20a ISO 6490 -1 
Phosphorus, min.  9 ISO 6491  
Total ash, min.  65 ISO 5984  
Acid insoluble ash, max.  4.0 ISO 5985  
a The values are on dry basis.  
 
4.2.2  The particle size shall be such that not less than 90 % by weight of the meal passes throu gh a-1 700 -
µm (1.70 mm) sieve.  
5 Hygiene  
Bone meal shall conform to the  maximum microbiological  limits specified  in Table 2 when tested in 
accordance  with the test methods specified therein.  
Table 2  — Microbiological limits in bone meal  
S/No Microorganism  Limit, max. Test method  
i Salmonella  spp Absent  ISO 6579 -1 
ii Spores of Bacillus anthracis  Absent  ISO 21871  
iii Clostridium botulinum  Absent  ISO/TS 17919  
 
6 Weights and measures  
Bone meal shall be weighed according to the weights and measures regulat ion of the destination country  
7 Packaging  
Bone meal  shall be packaged in suitable containers that are of sufficient strength, and sufficiently sealed to 
withstand reasonable handling without tearing, bursting or falling open. The containers shall be clea n and not 
previously used .  
4 © EAC 2019  – All rights reserved  
 8 Labelling   
In addition to the requirements in EAS 38, each package shall be legibly and indelibly labelled with the 
following:  
a) name of the product as ―Bone meal ‖; 
b) name and physical address of the manufacturer ; 
c) calcium and phosp horus content;  
d) net weight in metric units ; 
e) precautions for use;  
f) batch number /lot identification;  
g) manufacturing date ; and 
h) storage instru ctions . 
Bones from ruminants shall not be used for ruminants but can be used for monogastrics and vice versa.  
9 Samplin g 
Sampling shall be done in accordance with ISO 6497   
© EAC 2019  – All rights reserved  5 
 Bibliography  
EAS 231, Bone meal  feed specification  
 EAS 231: 2019    
 
© EAC  2019  – All rights reserved