EN 
ANNEX  
ANNEX I 
Templates for the reporting of the results of the annual surveys in demarcated areas carried out pursuant Article s 8 and 9  
Part A  
1. Template for the report ing of the results of annual surveys  1. Description of the DA  
2. Initial size of DA (ha)  
3. Updated size of DA (ha)  
4. Approach  
5. Zone  
6. Survey sites  
7. Risk areas identified  
8. Risk areas inspected  
9. Plant material / Commodity  
10. List of host plant species  
11. Timing  12. Survey details  
13. No of 
symptomatic 
samples 
analysed:  
i: Total  
ii: Positive  
iii: Negative  
iv: Undetermined  14. No of 
asymptomatic 
samples analysed:  
i: Total  
ii: Positive  
iii: Negative  
iv: Undetermined  15. 
Notification 
number of 
the outbreaks  
notified, as 
applicable, in 
accordance 
with 
Implementin
g Regulation 
(EU) 
2019/1715  
 
16. Comments  A) Number of visual examinations  
B) Total number of samples taken  
C) Type of traps (or other alternative method (e.g. 
sweep  nets))  
D) Number of traps (or other capturing method)  
E) Number of trapping sites, when different from 
data reported in (D)  
F) Type of tests (e.g. microscopic identification, 
PCR, ELISA, etc.)  
G) Total number of tests  
H) Other measures (e.g. sniffer dogs, drones, 
helicopters, awareness raising campaigns etc.)  Name  
Date of 
establishment  
Description  
Number  I) Number of other measures  
A B C D E F G H I i ii iii iv i ii iii iv 
Number  
Date  
 
                                 
                                 
                                 
                                 
  
 
 
 2. Instructions on how to fill in the template  
If this template is filled, the template in Part B of this Annex is not to be filled.  
For column 1: Indicate the name of the geographical area, outbreak number or any information that all ows identification of this  demarcated area  (DA) and the 
date when it was established.  
For column 2: Indicate the size of the DA before the start of the survey.  
For column 3: Indicate the size of the DA after the survey.  
For column 4: Indicate the approach:  Containment (C). Please, include as many rows as necessary, depending on the number of DA per pest and the 
approaches these areas are subject to.  
For column 5: Indicate the zone of the DA where the survey was carried out, including as many rows as necessary: Infested zone (IZ) or buffer zone (BZ), 
using separate rows. When applicable, indicate the area of the IZ where the survey was carried out (e.g . last 20 km adjacent to the BZ, around nurseries, etc.) 
in different rows.  
For column 6: Indicate the number and the description of the survey sites, by choosing one (or more) of the following entries for the description : 
1. Open air (production area): 1. 1. field (arable, pasture); 1.2. orchard/vineyard; 1.3. nursery; 1.4. forest;  
2. Open air (other): 2.1. private garden; 2.2. public sites; 2.3. conservation area; 2.4. wild plants in areas other than con servation areas; 2.5. other, with 
specification of t he particular case (e.g. garden centre, commercial sites that uses wood packaging material, wood industry, wetlands, irrigati on and 
drainage network, etc.);  
3. Physically closed conditions: 3.1. greenhouse; 3.2. private site, other than greenhouse; 3.3. p ublic site, other than greenhouse; 3.4. other, with 
specification of the particular case (e.g. garden centre, commercial sites that uses wood packaging material, wood industry).  
For column 7: Indicate which are the risk areas identified based on the biolog y of the pest(s), presence of host plants, eco -climatic conditions and risk 
locations.  
For column 8: Indicate the risk areas included in the survey, from those identified in column 7.  
For column 9: Indicate plants, fruits, seeds, soil, packaging material, wood, machinery, vehicles, water, other, specifying the specific case.  
For column 10: Indicate the list of plant species/genera surveyed , using one row per plant species/genera.  
For column 11: Indicate the months of the year when the survey was carried out . 
For column 12: Indicate the details of the survey, depending on the specific legal requirements of each pest. Indicate with N /A when the information of certain 
column is not applicable.  
For columns 13 and 14: Indicate the results, if applicable, providin g the information available in the corresponding columns. ‘Undetermined’ are those 
analysed samples for which no result was obtained due to different factors (e.g. below detection level, unprocessed sample -not identified, old,).   
 
 
 For column 15: Indicate the  outbreak notifications of the year when the survey took place for findings in the BZ. The outbreak notification number does n ot 
need to be included when the competent authority has decided that the finding is one of the cases referred to in Article 14(2 ), Article 15(2) or Article 16 of 
Regulation (EU) 2016/2031. In this case, indicate the reason for not providing this information in column 16 (‘Comments’).  
Part B  
1. Template for the reporting of the results of statistically based annual surveys  
 1. Descrip tion of  the DA  
2.  Initial size of DA (ha)  
3. Updated size of DA (ha)  
4. Approach  
5. Zone  6. Survey sites  
  
  
 6. Survey sites  
  
  
  7. Timing  A. Survey definition (input parameters for RiBESS+)  B. Sampling effort  C. Survey results  
25. Comments  8. Target 
population  
9. Epidemiological units  
10. Detection 
methods  
11. Sampling effectiveness  
12. Method sensitivity  13. Risk factors (activities, 
locations and areas)  
14. N° of epidemiological units inspected  
15. N° of visual examinations  
16. N° sample s 
17. N° of traps  
18. N° of trapping sites  
19. N° of tests  
20. N° of other measures  21. Results  22. 
Notification 
number of the 
outbreaks 
notified, as 
applicable, in 
accordance 
with 
Implementing 
Regulation 
(EU) 
2019/1715  
23 Achieved Confidence level  
24. Design prevalence  Name  
Date of establishment  
Description  
Number  
Host species  
Area (ha or other more 
relevant unit)  
Inspection units  
Description  
Units  
Visual examinations  
Trapping  
Testing  
Other methods  
Risk factor  
Risk levels  
N° of locations  
Relative risks  
Proportion of the host 
population  
Positive  
Negative  
Undetermined  
Number  
Date  
       
                                                                  
       
                                                                  
       
                                                                  
       
                                                                  
       
                                                                  
       
                                                                  
 
2. Instructions  on how to fill in the template   
 
 
 If this template is filled, the template in Part A of this Annex is not to be filled.  
Explain the underlying assumptions for the survey design per pest. Summarise and justify:  
- the target population, epidemiological unit and inspection units ; 
- the detection method and method sensitivity ; 
- the risk factor(s), indicating the risk levels and corresponding relative risks and proportions of host plant population . 
 
For column 1: In dicate the name of the geographical area, outbreak number or any information that allows identification of this demarcated area ( DA) and the 
date when it was established.  
For column 2: Indicate the size of the DA before the start of the survey.  
For column 3: Indicate the size of the DA after the survey.  
For column 4: Indicate the approach: Containment (C). Please, include as many rows as necessary, depending on the number of D A per pest and the 
approaches these areas are subject to.  
For column 5: Indicate the zone of the DA where the survey was carried out, including as many rows as necessary: Infested zone (IZ) or buffer zone (BZ), 
using separate rows. Whe re applicable, indicate the area of the BZ where the survey was carried out (e.g. last 20 km adjacent to the BZ, around nurseries, etc.) 
in different rows.  
For column 6: Indicate the number and the description of the survey sites, by choosing one (or more) of the following entries for the description : 
1. Open air (production area): 1.1 field (arable, pasture); 1.2. orchard/vineyard; 1.3. nursery; 1.4. forest;  
2. Open air (other): 2.1. private gardens; 2.2. public sites; 2.3. conservation area; 2.4. wild plants in areas other than co nservation areas; 2. 5. other, 
with specification of the particular case (e.g. garden centre, commercial sites that uses wood packaging material, wood indus try, wetlands, irrigation 
and drainage network, etc.);  
3. Physically closed conditions: 3.1. greenhouse; 3.2. private si te, other than greenhouse; 3.3. public site, other than greenhouse; 3.4. other, with 
specification of the particular case (e.g. garden centre, commercial sites that uses wood packaging material, wood industry).  
For column 7: Indicate the months of the year  when the surveys were carried out.  
For column 8: Indicate the chosen target population providing accordingly the list of host species/genera and area covered. T he target population is defined as 
the ensemble of inspection units. Its size is defined typica lly for agricultural areas as hectares, but could be lots, fields, greenhouses etc. Please justify the 
choice made in the underlying assumptions. Indicate the inspection units surveyed. 'Inspection unit’ means plants, plant part s, commodities, materials, p est 
vectors that had been scrutinised for identifying and detecting the pests.  
For column 9: Indicate the epidemiological units surveyed, indicating its description and unit of measurement. ‘Epidemiologic al unit’ means a homogeneous 
area where the interac tions between the pest, the host plants and the abiotic and biotic factors and conditions would result into the same epidemio logy, should 
the pest be present. The epidemiological units are a subdivision of the target population that are homogenous in terms  of epidemiology with at least one host 
plant. In some cases the whole host population in a region/area/country may be defined as epidemiological unit. They could be  NUTS regions, urban areas, 
forests, rose gardens or farms, or hectares. The choice  of the epidemiological units hasto  be justified in the underlying assumptions.  
For column 10: Indicate the methods used during the survey including the number of activities in each case, depending on the specific legal requirements of 
each pest. Indicate with N/A  when the information of certain column is not available.   
 
 
 For column 11: Indicate an estimation of the sampling effectiveness. Sampling effectiveness means the probability of selectin g infected plant parts from an 
infected plant. For vectors, it is the eff ectiveness of the method to capture a positive vector when it is present in the survey area. For soil, it is the effectivenes s 
of selecting a soil sample containing the pest when the pest is present in the survey area.  
For column 12: ‘Method sensitivity’ means the probability of a method to correctly detect pest presence. The method sensitivity is defined as the probability 
that a truly positive host tests positive. It is the multiplication of the sampling effectiveness (i.e. probability of select ing infec ted plant parts from an infected 
plant) by the diagnostic sensitivity (characterised by the visual inspection and/or laboratory test used in the identificatio n process).  
For column 13: Provide the risk factors in different rows, using as many rows as neces sary. For each risk factor indicate the risk level and corresponding 
relative risk and proportion of host population.  
For column B: Indicate the details of the survey, depending on the specific legal requirements for each pest. Indicate with N /A when the i nformation of certain 
column is not applicable. The information to be provided in these columns is related to the information included in the colum n 10 ‘Detection methods’.  
For column 18: Indicate the number of trapping sites in case this number differs f rom the number of traps (Column 17) (e.g. the same trap is used in different 
places).  
For column 21: Indicate the number of samples found positive, negative or undetermined. ‘Undetermined’ are those analysed sam ples for which no result was 
obtained due to different factors (e.g. below detection level, unprocessed sample -not identified, old).  
For column 22: Indicate the outbreak notifications of the year when the survey took place. The outbreak notification number d oes not need to be included 
when the compet ent authority has decided that the finding is one of the cases referred to in Article 14(2), Article 15(2) or Article 16 of Regulation (EU) 
2016/2031. In this case, indicate the reason for not providing this information in column 25 (‘Comments’).  
For colum n 23: Indicate the sensitivity of the survey, as defined in ISPM 31. This value of the achieved confidence level of pest free dom is calculated based 
on the examinations (and/or samples) performed given the method sensitivity and the design prevalence.  
For column 24: Indicate the design prevalence based on a pre -survey estimate of the likely actual prevalence of the pest in the field. The design prevalence is 
set as a goal of the survey and corresponds to the compromise the risk managers are making between t he risk of having the pest and the resources available for 
the survey. Typically, for a detection survey a value of 1% is set.  
 
   
 
 
 ANNEX  II 
Level s of destructive sampling  
 
Number of plants in the lot  Level of destructive 
sampling (number of plants 
to be cut) 
1 – 4 500  10 % of lot size  
> 4 500  450