Ammonium Hydrogen Sulfite Water  
亜硫酸水素アンモニウム水  
 
Standards for Use  
Permitted for use in grape juice used for wine production and grape wine only . 
Shall be used at not more than 0.2 g/L in grape wine as ammonium hydrogen sulfite . 
When Ammonium Hydrogen Sulfite Water is used in grape juice used for wine production, 
this additive is considered to be used in grape wine.  
Shall not remain at a level not less th an 0.35 g/kg as sulfur dioxide in grape wine  
(excluding squeezed grape juice used for wine production containing  not less than 1  % 
(volume ) of ethanol  and the concentrate of such squeezed grape juice ). 
 
Compositional Specifications  
Substance Name  Ammonium  Hydrogen Sulfite Water  
Definition  
Ammonium Hydrogen Sulfite Water is a solution consisting mainly of ammonium 
hydrogen sulfite.  
Content  
Ammonium Hydrogen Sulfite Water  contains not less than 13.0% of ammonium 
hydrogen sulfite ( NH 4HSO 3 = 99.11 ). 
Description   Ammonium Hydrogen Sulfite Water  occurs as a light yellow  liquid . 
Identification  
(1) Ammonium Hydrogen Sulfite Water responds to the test for Ammonium Salt and 
all the tests for Sulfite and Bisulfite in the Qualitative Tests.  
(2) Ammonium Hydro gen Sulfite Water contains not less than 2. 2 % as ammonia  
(NH 3=17.03).  
Weigh accurately about 0.5 g of Ammonium Hydrogen Sulfite Water , add 25 mL of water  
to dissolve it, and a dd 10 mL of sodium hydroxide solution (2 in 5 ). Immediately connect 
the flask containing this solution to a previously assembled distillation apparatus. The 
apparatus is equipped with a condenser joined to a delivery tube with a spray trap and 
the lower end of the condenser is immersed in 30 mL of 0.1 m ol/L sulfuric acid, exactly 
measured, in a receiver.  Heat and distill ammonia into the sulfuric acid until about 25 Attachment  mL of the distillate  is obtained.  Titrate the excess sulfuric acid in the receiver with 0.2 
mol/L sodium hydroxide (indicator: 3 drops of me thyl red TS). Determine the amount of 
ammonia  by the formula.  
Each mL of 0.1 mol/L sulfuric acid = 3.406 mg of NH 3 
Purity  
(1) Lead   Not more than 5 μg/g of NH 4HSO 3 as Pb ( an amount equivalent to 8.0  g of 
ammonium hydrogen sulfite ( NH 4HSO 3), Method 5, Control Solution: Lead Standard 
Solution 4.0 mL, Flame Method) . 
Sample Solution   To the specified amount of Ammonium Hydrogen Sulfite Water , 
add 20mL of diluted hydrochloric acid  (1 in 4), and boil gently for 5 minutes with a watch 
glass covering it. Allow  to cool.  Use this solution as the sample solution.  If the sample 
does not dissolve completely, evaporate it to dryness, and add 20 mL of diluted 
hydrochloric acid (1 in 4) to the residue. Boil gently for 5 minutes, allow to cool, and use 
this solution as the sample solution.  
(2) Arsenic   Not more than 3 μg/g of NH 4HSO 3 as As (an amount equivalent to 5.0 g 
of ammonium hydrogen sulfite ( NH 4HSO 3), Standard Color: Arsenic Standard Solution 
3.0 mL, Apparatus B).  
Test Solution   To the specified amount of Ammonium Hydrogen Sulfite Water, add 
water to make 50 mL. Measure  10 mL of this solution, add 2 mL of sulfuric acid, and 
heat on a water bath until sulfur dioxide is no longer evolved. Evaporate to about 2 mL, 
and add water to ma ke 10 mL. Use 5 mL of this solution as the test solution.  
Residue on Ignition   Not more than 0.2%  per ammonium hydrogen sulfite ( NH 4HSO 3) 
(10 g) . 
Assay    Weigh accurately about 0.3 g of Ammonium Hydrogen Sulfite Water, and 
proceed as directed under Sulfite Determination.  
Each mL of 0.05 mol/L iodine = 4.955 mg of NH 4HSO 3