COMMISSION IMPLEMENTING REGUL ATION (EU) 2023/1032 
of 25 May 2023
establishing measures to prev ent the introduction into and the spread within the Union territory of 
Tomato brown rugose fruit virus (ToBRFV) and amending Implementing Regulation (EU) 2020/1191 
THE EUR OPEAN COMMISSION,
Havi ng regar d to the Treaty on the Functioning of the European Union,
Havi ng regard to Regulation (EU) 2016/2031 of the European Parliament and the Council of 26 October 2016 on 
prote ctive measures against pests of plants, amending Regulations (EU) No 228/2013, (EU) No 652/2014 and (EU) 
No 1143/2014 of the European Parliament and of the Council and repealing Council Directives 69/464/EEC, 74/647/EEC, 
93/85/EEC, 98/57/EC, 2000/29/EC, 2006/91/EC and 2007/33/EC (1), and in particular Article 30(1) thereof,
Havi ng regard to Regulation (EU) 2017/625 of the European Parliament and of the Council of 15 March 2017 on official 
controls and other official activities perf ormed to ensure the application of food and feed law, rules on animal health and 
welf are, plant health and plant protection products, amending Regulations (EC) No 999/2001, (EC) No 396/2005, (EC) 
No 1069/2009, (EC) No 1107/2009, (EU) No 1151/2012, (EU) No 652/2014, (EU) 2016/429 and (EU) 2016/2031 of the 
European Parliament and of the Council, Council Regulations (EC) No 1/2005 and (EC) No 1099/2009 and Council 
Directives 98/58/EC, 1999/74/EC, 2007/43/EC, 2008/119/EC and 2008/120/EC, and repealing Regulations (EC) 
No 854/2004 and (EC) No 882/2004 of the European Parliament and of the Council, Council Directives 89/608/EEC, 
89/662/EEC, 90/425/EEC, 91/496/EEC, 96/23/EC, 96/93/EC and 97/78/EC and Council Decision 92/438/EEC (Offici al 
Controls Regulation) (2), and in particular Article 22(3) and Article 52 thereof,
Whereas:
(1) Tomato brown rugose fruit virus (ToBRFV) (‘the specifi ed pest ’) is currently not listed as a Union quarantine pest, or 
as a Union regulate d non-quarantine pest, in Commission Implementing Regulation (EU) 2019/2072 (3). However it 
fulfils the criteria set out in Subsection 2 of Section 3 of Annex I to Regulation (EU) 2016/2031 for a preliminar y 
assessment to identify pests which provisionally qualify as a Union quarantine pest requir ing temporar y measures 
as refer red to in Article 30(1) of that Regulation.
(2) Commission Imple menting Regulation (EU) 2020/1191 (4)established measures to prevent the introduction into 
and the spread within the Union of the specif ied pest. That Regulation is to expire on 31 May 2023 .
(3) After the adoptio n of Implementing Regulation (EU) 2020/1191, more recent scientific information has been 
collect ed on the spread of the specified pest and on testing methods and the audits carried out by the Commission 
services provided feedback on the implementation of the provisions and on their impact on the protect ion against 
the spread of the disease. That justif ies the need for the adop tion of a new act with more detailed measures than 
those provid ed for in that Regulation.
(4) In order to ensure the most proactive approach concer ning phytosanitar y prot ection, measures should be 
established for situations where any person within the Union territory suspects or becomes aware of the presence of 
the specifi ed pest, and with regar ds to the corresponding notification to the compet ent author ity, and the actions to 
be taken by it.
(1)OJ L 317, 23.11.2016, p. 4.
(2)OJ L 95, 7.4.2017, p. 1.
(3)Commission Implementing Regulation (EU) 2019/2072 of 28 November 2019 establishing unif orm conditions for the 
impl ementation of Regulation (EU) 2016/2031 of the European Parliament and the Council, as regar ds protective measures agai nst 
pests of plants, and repealing Commission Regulation (EC) No 690/2008 and amending Commission Implementin g Regulation 
(EU) 2018/2019 (OJ L 319, 10.12.2019, p. 1).
(4)Commission Implementing Regulation (EU) 2020/1191 of 11 August 2020 establishing measures to prevent the introduction into and 
the spread within the Union of Tomat o brown rugose fruit virus (ToBRFV) and repealing Implementing Decision (EU) 2019/1615 
(OJ L 262, 12.8.2020, p. 6).EN Offi cial Jour nal of the European Union L 139/34 26.5.2023  (5) Where the presence of the specifi ed pest is officially conf irmed in the territory of a Member State , the compet ent 
author ity of the Member State concer ned should establish a demarcated area to ensure the eradication of that pest 
and the prevention of its spread to the rest of the Union territory. In order to ensure the most appropr iate and 
propor tionat e approach, differ ent rules for the demarcation should be adopt ed for the case where the presence of 
the specified pest is conf irmed in production sites with physical prot ection, because of the reduced phytosanitar y 
risk resulting from that protection.
(6) In order to ensure a more proactive approach for the protect ion of the Union territory from the specified pest, 
Member States should conduct annual surveys for the presence of the specifi ed pest in their territory.
(7) Rules should be established for the mov ement within the Union of seeds of Solanum lycopersicum L. and hybr ids 
thereof, and of Capsicum spp. (‘specif ied seeds ’), and of plants for planting, other than specified seeds, of Solanum 
lycopersicum L. and hybrids thereof and of Capsicum spp. (‘specif ied plants for planting’), because those seeds and 
other plants for planting are most likely to host and spread the specifi ed pest.
(8) Those rules should include, as appropr iate for the type of plant and its production, pest freedom in the production 
site, visual inspection, sam pling and testing, appropr iate handling of lots, and measures on mother plants. Such an 
approach is necessar y in order to adapt to the technical circumstances of each case of production and mov ement of 
the specifi ed seeds and specified plants.
(9) In particular , all lots of specif ied seeds should undergo sampli ng and testing by the compet ent author ity in order to 
detect the presence of the specified pest. Those specified seeds should also be tested by the compet ent author ity or 
professiona l operator , as applicable, prior to further processing, and have been found, according to those tests, to be 
free from the specified pest. This is necessar y for the prot ection of the Union territory from the specified pest, as the 
specifi ed seeds are the starting mater ial for the production of all respective plants.
(10) In order to prote ct the Union territory from the specified pest, requirements should be set out for the introduction 
into the Union of specified seeds and specified plants for planting from third countr ies. Those requirements should 
be similar to the ones concer ning the mov ement of the specifi ed seeds and specifi ed plants for planting within the 
Union, in order to ensure a non-discr iminato ry approach.
(11) It is propor tionat e to excep t from those requirements specifi ed seeds and specifi ed plants belonging to varieties 
which are known to be resistant to the specifi ed pest, because the respective phyt osanitar y risk is reduced to an 
acceptable level in the case of those plants. Members State s should notify to the Commission and to the other 
Members States a list of those resistant varieties, regularly updat ed.
(12) In order to ensure effective official controls against the entr y of the specifi ed pest into the Union, at least 20 % of the 
consignments of specif ied seeds and of specified plants for planting should be sampled and tested by the compet ent 
author ity at the border control posts of first arrival into the Union, or at a control point as refer red to in Commission 
Delegat ed Regulation (EU) 2019/2123 (5). For consignments of specifi ed seeds and specifi ed plants for planting 
originating in Israel and China, that sam pling and testing rate should be 50 % and 100 %, respectively , due to the 
higher number of inter cepti ons of the specifi ed pest in commodities originating from those third countr ies.
(13) In order to allow sufficient time for the third countr ies, the compet ent author ities and the profess ional operat ors to 
adapt to the provis ions of this Regulation, it should apply from 1 September 2023 . For this reason, and in order to 
avoid any legal gap, the expiration of the application of Implementing Regulation (EU) 2020/1191 should be 
extende d from 31 May 2023 to 31 August 2023 .
(5)Commission Delegat ed Regulation (EU) 2019/2123 of 10 October 2019 supplementing Regulation (EU) 2017/625 of the European 
Parliament and of the Council as regar ds rules for the cases where and the conditions under which identity checks and physical chec ks 
on certain goods may be perfor med at control points and documentar y chec ks may be perf ormed at distance from border control 
posts (OJ L 321, 12.12.2019, p. 64).EN Offi cial Jour nal of the European Union 26.5.2023 L 139/35  (14) The full assessment of the specifi ed pest is still pending, in order to determine its risk for the Union territory. For this 
reason, this Regulation should apply until 31 December 2024 , in order to allow time for that assessment to happen 
by then.
(15) In order to swif tly address the phytosan itary risk of the specifi ed pest, the rules of this Regulation should become 
applicable after the shor test possible dela y. Theref ore, this Regulation should enter into force on the day followi ng 
that of its publication.
(16) The measures provid ed for in this Regulation are in accordance with the opinion of the Standing Committ ee on 
Plants, Animals, Food and Feed,
HAS ADOPTED THIS REGUL ATION:
Article 1
Subject matter
This Regulation establishes measures to prevent the introduction into and the spread within the Union of Tomat o brown 
rugose fruit virus (ToBRFV).
Article 2
Def initions
For the purpose of this Regulation, the followi ng definitions shall apply:
(a) ‘specif ied pest’ means Tomato brown rugose fruit virus (ToBRFV);
(b) ‘specif ied plants’ means plants of Solanum lycopersicum L. and hybrids thereof, and of Capsicum spp., other than specifi ed 
seeds and specifi ed fruits;
(c) ‘specif ied plants for planting’ means plants for planting of Solanum lycopersicum L. and hybr ids thereof and of Capsicum 
spp., other than specified seeds;
(d) ‘specif ied seeds ’ means seeds of Solanum lycopersicum L. and hybrids thereof, and of Capsicum spp.;
(e) ‘specif ied fruits’ means fruits of Solanum lycopersicum L. and hybr ids thereof, and of Capsicum spp.
Article 3
Prohibitions concer ning the specif ied pest
The specif ied pest shall not be introduced into, moved within, or held, multiplied or released within the Union territory .
Article 4
Measures in the case of suspicion or awareness of the presence of the specif ied pest
1. Any person within the Union territory who suspects or becomes awar e of the presence of the specifi ed pest, shall 
immediately inform the compet ent author ity and provid e it with all relevant information concer ning the presence, or 
suspecte d presence, of that pest.
2. On receiving such information, the compet ent author ity shall:
(a) immediately record the information provi ded;
(b) take all necessar y measures to confir m or refute the presence or suspect ed presence of the specifi ed pest ;EN Offi cial Jour nal of the European Union L 139/36 26.5.2023  (c) ensure that any person having specified plants, specifi ed seeds or specified fruits whic h may be infec ted with the 
specifi ed pest under its control, is immediate ly informed of:
(i) the presence or the suspecte d presence of the specified pest ; and
(ii) of the possible risks associated with the specifi ed pest and of the measures to be take n.
Article 5
Sur veys on the presence of the specif ied pest
1. The compet ent author ities shall conduct annual surveys for the presence of the specifi ed pest in their territory .
2. Those surveys shall:
(a) include sampling and testing as laid down in the Annex; and
(b) be based on:
(i) the assessed risk of introduction and spread of the specified pest into and within the Member State concer ned; and
(ii) sound scientifi c and technical principles, with regar d to the possibility to detec t the specifi ed pest.
3. By 30 Apr il of each year, Member State s shall notify the Commission and the other Member States the results of the 
surveys carried out during the preceding calendar year .
Article 6
Measures in case of conf irmed presence of the specif ied pest
1. Where the presence of the specifi ed pest is officially conf irmed in the territory of a Member State, the compet ent 
author ity of the Member State concer ned shall ensure that appropr iate measures are take n to eradicate the specifi ed pest in 
accordance with Article 17 of Regulation (EU) 2016/2031.
That compet ent author ity shall take the measures laid down in paragraphs 2 and 3 of this Article, unless the conditions set 
out in Article 18(4) of Regulation (EU) 2016/2031 are fulfilled with regard to the specified pest.
Measures laid down in paragraphs 2 and 3 shall not apply to specified plants for planting of varieties whic h are known to be 
resistant to the specifi ed pest. Members States shall notify to the Commission and to the other Members States a list of those 
resistant varieties, regularly updat ed.
2. The compet ent author ity shall establish without dela y a demarcat ed area as follows:
(a) if the specified pest is present in production sites with physical protection, the demarcated area shall consist of at least 
the production site where the specifi ed pest has been found;
(b) if the specifi ed pest is present in production sites other than those referred to in point (a), the demarcated area shall 
consist of:
(i) an infested zone including at least the production site where the specifi ed pest has been found;
(ii) a buffer zone of at least 30 m surrounding the infested zone.
3. In the demarcated area, the compet ent author ity, or the professiona l operat or under the official super vision of the 
compet ent author ity, shall:
(a) for production sites intende d for the production of specif ied plants for planting or for the production of specif ied seeds:
(i) immediately remove and destro y all infected lots of the specif ied plants for planting and, where applicable, their 
growin g medium and the specified seeds originating from those lots. That removal and destr uction shall be 
carried out in such a way that there is no risk of spreading the specifi ed pest ;EN Offi cial Jour nal of the European Union 26.5.2023 L 139/37  (ii) apply specifi c hygi ene measures on personnel, production site structures, tools and machi nery, mat erials and 
means of transpor t, to prevent the spread of the specifi ed pest to the other lots present in the production site and 
to the successive crops of the specifi ed plants or to other production sites;
(iii) destro y or treat the growing medium, at least at the end of the cropping season such that there is no identifiable 
risk of spreading the specifi ed pest ;
(b) for production sites intended for the production of specifi ed fruits:
(i) remove and destro y all infected specif ied plants from the production site, at least at the end of the cropping season. 
The remo val shall be carried out in such a way that there is no identifiable risk of spreading the specifi ed pest ;
(ii) apply specifi c hygi ene measures on personnel, production site structures, tools and machi nery, mat erials and 
means of packing and transpor t of the fruits, to prevent the spread of the specif ied pest to successive crops of the 
specifi ed plants or to other production sites;
(iii) destro y or treat the growing medium at least at the end of the cropping season such that there is no identif iable 
risk of spreading the specifi ed pest.
Article 7
Movement of the specif ied plants for planting within the Union
1. The specified plants for planting may only be moved within the Union if they are accompan ied by a plant passpor t, 
issued after the compet ent author ity or the profe ssional operat or concer ned has concluded all of the followi ng conditions 
are fulfilled :
(a) the specified plants for planting derive from specifi ed seeds which compl y with the requirements laid down in Articles 
8 and 10;
(b) the specified plants for planting have been grown in a production site where the specified pest is known not to occur , 
on the basis of official inspections carried out at the appropr iate time to detect the specifi ed pest ;
(c) the specifi ed plants for planting that have displa yed symp toms of the specifi ed pest have been subjected to sampling 
and testing by the compet ent author ity, and those tests have shown that those plants are free from the specified pest ;
(d) the lots of the specifi ed plants for planting have been kept separate from other lots of specified plants, by applying 
appropr iate hygiene measures.
Sampli ng for testin g, as referred to in this paragraph, shall be carried out as set out in the Annex.
2. The conditions laid down in paragraph 1 for issuance of a plant passpor t shall not apply to specifi ed plants for 
planting of varieties whic h are known to be resistant to the specified pest. Members States shall notify to the Commission 
and to the other Members States a list of those resistant varieties, regularly updat ed.
Article 8
Movement of the specif ied seeds within the Union
1. Specified seeds may only be mov ed within the Union, if they are accompan ied by a plant passpor t issued after the 
compet ent author ity or the profes sional operat or concer ned has concluded that the followi ng conditions are fulfilled:
(a) the mother plants of the specif ied seeds have been produced in a production site where the specified pest is kno wn not 
to occur , on the basis of official inspections carried out at the appropr iate time to detect the specifi ed pest ;EN Offi cial Jour nal of the European Union L 139/38 26.5.2023  (b) in the case of a specifi ed seed lot originating from more than 30 mother plants that specified seed lot, prior to 
processing, has undergone sampli ng and testing as set out in the Annex by the compet ent author ity for the presence of 
the specifi ed pest, or has been subjecte d by the profess ional operators to sam pling and testing under the official 
super vision of the compet ent author ity, and has been found, according to those tests, to be free from the specified 
pest. Any presence of the specified pest has been notifi ed to the compet ent author ity and the infect ed specified seed 
lots shall not move within the Union territory ;
(c) in the case of a specifi ed seed lot originating from less than or equal to 30 mother plants, sampling and testing, as set 
out in the Annex, have been carried out by the compet ent author ity for the presence of the specifi ed pest, or by the 
professional operat ors under the official super vision of the compet ent author ity on the specified seeds or on each 
mother plant of those specifi ed seeds. Specifi ed seeds or mother plants have been found, according to those tests, to 
be free from the specified pest. Any presence of the specifi ed pest has been notified to the compet ent author ity and the 
specifi ed seed lots originating from the infec ted mother plants shall not mov e within the Union territory;
(d) in case of suspicion of the presence of the specifi ed pest, that sam pling and testing shall only be carried out by the 
compet ent author ities in accordance with Article 87(3), point (c), of Regulation (EU) 2016/2031;
(e) the origin of all lots of specified seeds are recorded and documente d.
2. By way of derog ation from paragraph 1, points (a), (b), (c) and (d), specified seeds that have been harveste d prior to 
31 August 2023 and before their first mov ement within the Union have been found by the compet ent author ity or the 
professiona l operat or concer ned to compl y with the requirements of Implementing Regulation (EU) 2020/1191, may 
mov e within the Union accompan ied by a plant passpor t attesting compliance with those requirements.
3. The lots of specified seeds mov ed for the first time within the Union since 1 Apr il 2021 , and which have been tested 
before 30 Sept ember 2020 with the enzyme-linked immunosorbent assa y (ELISA) method, shall be tested again with a 
testing method, other than ELISA, as refer red to in point 3 of the Annex.
4. Sampli ng and testing shall be carried out as set out in the Annex.
5. The conditions laid down in paragraphs 1 and 2 for issuance of a plant passpor t shall not apply to specifi ed seeds of 
varieties which are kno wn to be resistant to the specifi ed pest. Members States shall notify to the Commission and to the 
other Members States a list of those resistant varieties, regularly updated.
Article 9
Ιntroduction into the Union of the specif ied plants for planting
1. Specified plants for planting, other than the ones belonging to varieties which are kno wn to be resistant to the 
specifi ed pest, originating from third countr ies may only be introduced into the Union if they are accompan ied by a 
phytosanitar y certificate which, under the heading ‘Additional declaration’, includes the followi ng elements:
(a) an official statement that the specifi ed plants for planting derive from seeds whic h compl y with the requirements laid 
down in Article 10;
(b) an official statement that the specifi ed plants for planting have been produced in a production site which is register ed 
and super vised by the national plant prot ection organisation in the countr y of origin and known to be free from the 
specifi ed pest on the basis of offici al inspections, sam pling and testing carried out at the appropr iate time to detect 
that pest ;
(c) the name of the registered production site.
2. Specified plants for planting of varieties which are known to be resistant to the specifi ed pest, originating from third 
countr ies, may only be introduced into the Union if they are accompanied by a phytosanitar y certificate, whic h, under the 
heading ‘Additional Declaration’ , conf irms that resistance.EN Offi cial Jour nal of the European Union 26.5.2023 L 139/39  Article 10
Introduction into the Union of the specif ied seeds
1. Specified seeds originating from third countr ies, other than the ones belonging to varieties which are kno wn to be 
resistant to the specifi ed pest, may only be introduced into the Union if they are accompan ied by a phytosanitar y 
certificate, whic h, under the heading ‘Additional Declaration’, contains all of the followi ng:
(a) an official statement that all of the followi ng conditions have been fulfilled:
(i) the mother plants of the specifi ed seeds concer ned have been produced in a production site where the specifi ed 
pest is known not to occur , on the basis of official inspections carried out at the appropr iate time to detect the 
specifi ed pest ;
(ii) in the case of a specifi ed seed lot originating from more than 30 mother plants, that specified seed lot, prior to 
processing, has undergone official sampling and testing for the specifi ed pest as set out in the Annex and have 
been found, according to those tests, to be free from the specifi ed pest ;
(iii) in the case of a specified seed lot originating from less than or equal to 30 mother plants, sam pling and testing, as 
set out in the Annex have been carried out on the specif ied seeds, or on each individual mother plant of those 
specifi ed seeds. Specified seeds or mother plants have been found, according to those tests, to be free from the 
specifi ed pest ;
(b) information ensur ing the traceability of the production site of the mother plants.
2. Specified seeds of varieties which are known to be resistant to the specified pest, originating from third countr ies, 
may only be introduced into the Union if they are accompan ied by a phytosan itary certificate, whic h, under the heading 
‘Additional Declaration’ confir ms that resistance.
3. By way of derog ation from paragraph 1, point (a), specified seeds that have been harvested prior to 31 August 2023
and before their introduction within the Union have been found to compl y with the requirements of Implementing 
Regulation (EU) 2020/1191, may be introduced into the Union territory accompan ied by a phytosan itary certificate 
indicating under the heading ‘Additional Declaration’ , the follo wing statement: ‘These seeds have been harvested before 
31 August 2023 and have been found to compl y with Regulation (EU) 2020/1191’.
Article 11
Official checks on introduction into the Union
At least 20 % of the consignments of specified seeds and of specified plants for planting shall be sampled and tested by the 
compet ent author ity at the border control posts of first arrival into the Union, or at a control point as refer red to in 
Article 2 of Delegat ed Regulation (EU) 2019/2123, as set out in the Annex to this Regulation.
For consignments of specifi ed seeds and specified plants for planting originating in Israel and China, that sampling and 
testing rate shall be 50 % and 100 %, respectively .
Article 12
Amendment of Implementing Regulation (EU) 2020/1191
In Article 12 of Implementing Regulation (EU) 2020/1191, the date ‘31 May 2023 ’ is replaced by ‘31 August 2023 ’.EN Offi cial Jour nal of the European Union L 139/40 26.5.2023  Article 13
Entr y into force and date of application
This Regulation shall enter into force on the day following that of its publication in the Official Journal of the European Union.
It shall apply from 1 Sept ember 2023 until 31 December 2024 .
This Regulation shall be binding in its entirety and directly applicable in all Member States.
Done at Brussels, 25 May 2023 .
For the Commission
The President
Ursula VON DER LEYENEN Offi cial Jour nal of the European Union 26.5.2023 L 139/41  ANNEX 
1.Sampling schemes of specif ied seeds, other than those of varieties which are kno wn to be resis tant to the 
specif ied pest
Sampli ng of seeds for testing shall be carried out under the follo wing sam pling sche mes depending on the seed lots as 
refer red to in the relevant tables of the Internatio nal Standards for Phyt osanitar y Measures No 31 Methodologies for 
sampli ng of consignments (ISPM31):
a)for a seed lot originating from less than or equal to 30 mother plants:
— application of a hypergeometr ic sampling scheme able to identify with 95 % reliability a level of presence of 
infected plants of 10 % or above; or
— testin g of each mother plant of the seed lot;
b)for a seed lot less than or equal to 3 000 seeds: application of a hyperg eometr ic sampling scheme able to identify 
with 95 % reliability a level of presence of infec ted plants of 10 % or above;
c)for a seed lot of more than 3 000 but less than or equal to 30 000 seeds: application of a sam pling sche me able to 
identify with 95 % reliability a level of presence of infected plants of 1 % or above;
d)for a seed lot of more than 30 000 seeds: application of a sampling scheme able to identify with 95 % reliability a 
level of presence of infect ed plants of 0,1 % or above.
Sub samples shall consist of 1 000 seeds maximum for Polymerase Chain Reaction (PCR) methods.
2.Sampling schemes of specif ied plants, other than those varieties which are kno wn to be resis tant to the 
specif ied pest
a)In the case of those specifi ed plants, at least one sample of up to 200 young leav es from the top of the plant, or 
sepals from fruits, shall be collect ed per site of production and per cultivar when applicable.
b)In the case of symp tomatic plants, sampling for testing shall be perf ormed on at least 3 symp tomatic leaves.
c)In case of testing of mother plants, young leaves from the top of plant, or sepals from fruits, shall be collect ed as 
appropr iate.
3.Testing methods for detecting and identifying the specif ied pest on seeds, other than those of varieties which 
are kno wn to be resist ant to the specif ied pest
One of the followi ng testing methods shall be used for the detection of the specified pest on the specif ied seeds:
— real-time RT-PCR using the primers and probes descr ibed in the ISF prot ocol (2020) (1);
— real-time RT-PCR using primers and probe of Menzel and Wint er (2021) (2);
— real-time RT-PCR using primers and probe from Bernabé-Or ts et al. (2021) (3).
In case of a positive result of the detection test, a second testing method, differ ent from the one used for detection, shall 
be carried out with one of the real-time RT-PCR methods listed abov e, using the same sample to conf irm the 
identif ication. In the case of inconsistency between detection and identif ication results obtained for coated seeds, the 
coating of the seeds shall be removed and the seeds shall be retested, if applicable.
(1)ISF (2020) Detec tion of Infec tious Tomato brown rugose fruit virus (ToBRFV) in Tomato and Pepper Seed. https://worldseed.org/our - 
work/seed-health/ishi-methods/ , version1.5, upload 29/03/2023.
(2)Menzel, W. & Winter , S. (2021). Identification of novel and known tobamo viruses in tomat o and other solanaceous crops using a new 
pair of generic primers and development of a specific RT- qPCR for ToBRFV . Acta Horticultur ae 1316, pp. 143 -148.
(3)Bernabé-Or ts, J.M., Torre, C., Méndez-López, E., Hernando, Y., Aranda, M.A. (2021) New Resources for the Specific and Sensitive 
Detec tion of the Emerging Tomat o Brown Rugose Fruit Virus. Viruses 13, 1680.EN Offi cial Jour nal of the European Union L 139/42 26.5.2023  4.Testing methods for detecting and identifying the specif ied pest on the specif ied plants, other than those of 
varieties which are kno wn to be resist ant to the specif ied pest , and on the specif ied fruits
One of the follo wing testing methods shall be carried out for the detection of the specif ied pest on the specifi ed plants, 
other than those varieties whic h are known to be resistant to the specifi ed pest, and on the specified fruits:
— ELISA, only for symp toma tic mater ial;
— conven tional RT-PCR using the primers of Alkowni et al. (2019) (4);
— conven tional RT-PCR using the primers of Rodriguez-Mendoza et al. (2019) (5);
— real-time RT-PCR using the primers and probes descr ibed in the ISF prot ocol (2020) (6);
— real-time RT-PCR using primers and probe of Menzel and Wint er (2021) (7);
— real-time RT-PCR using primers and probe from Bernabé-Or ts et al. (2021) (8).
In case of a positive result of the detection test, a second testing method, differ ent from the one used for detection, shall 
be carried out with one of the RT-PCR methods listed abov e, using the same sample to conf irm the identification.
(4)Alkowni, R, Alabdallah, O., Fadda, Z. (2019) Molecular identification of tomat o brown rugose fruit virus in tomat o in Palest ine. Journal 
of Plant Pathology 101(3), 719–723.
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