ELI: http://data.europa.eu/eli/reg_impl/2024/260/oj 1/11COMMISSION IMPLEMENTING REGUL ATION (EU) 2024/260 
of 12 Januar y 2024
concer ning the author isation of cumin essential oil from Cuminum cyminum L., sweet fennel tincture 
from Foeniculum vulg are Mill. ssp. vulg are var. dulce, dong quai tincture from Ang elica sinensis (Oliv .) 
Diels, parsley tincture from Petrosel inum crispum (Mill.) Fuss, star anise tincture from Illicium verum 
Hook f., asafoetida essential oil from Ferula-assa-f oetida L., dill herb essential oil from Anethum 
graveolens L. and dill tincture from Aneth um graveolens L. as feed additiv es for certain animal species 
(Text with EEA relevance) 
THE EUR OPEAN COMMISSION,
Havi ng regar d to the Treaty on the Functioning of the European Union,
Havi ng rega rd to Regulation (EC) No 1831/2003 of the European Parliament and of the Council of 22 Sept ember 2003 on 
additives for use in animal nutr ition (1), and in particular Article 9(2) thereof,
Whereas:
(1) Regulation (EC) No 1831/2003 provid es for the author isation of additives for use in animal nutr ition and for the 
grounds and procedures for granting such author isation. Article 10(2) of that Regulation provides for the 
re-evaluation of additives author ised pursuant to Council Directive 70/524/EEC (2).
(2) The substances cumin essential oil from Cuminum cyminum L., sweet fennel tincture from Foeniculum vulgare Mill. ssp. 
vulgare var. dulce, parsle y tincture from Petroselinum crispum (Mill.) Fuss, star anise tincture from Illicium verum Hook 
f., dill tincture from Anethum graveolens, dong quai tincture from Angelica sinensis (Oliv .) Diels, asafoetida essential oil 
from Ferula-assa-foetida L., and dill herb essential oil from Anethum graveolens L. were author ised without a time limit 
in accordance with Directive 70/524/EEC as feed additives for all animal species. These substances were 
subsequently entered in the Registe r of Feed Additives as existing products belonging to the cate gory ‘sensor y 
additives ’ and to the functional group ‘flavouri ng compo unds ’, in accordance with Article 10(1), point (b), of 
Regulation (EC) No 1831/2003.
(3) In accordance with Article 10(2) of Regulation (EC) No 1831/2003 in conjunction with Article 7 thereof, an 
application was submitted for the author isation of cumin essential oil from Cuminum cyminum L., sweet fennel 
tincture from Foeniculum vulgare Mill. ssp. vulgare var. dulce, parsley tincture from Petroselinum crispum (Mill.) Fuss, 
star anise tincture from Illicium verum Hook f., dill tincture from Anethum graveolens, dong quai tincture from 
Angel ica sinensis (Oliv .) Diels, asafoetida essential oil from Ferula-assa-foetida L., and dill herb essential oil from 
Anethum graveolens L. for all animal species, requesting the additives to be classified in the additive categor y ‘sensor y 
additives ’ and in the functional group ‘flavouring compounds’. That application was accompan ied by the particulars 
and documents required under Article 7(3) of Regulation (EC) No 1831/2003. The applicant subsequently withdrew 
the application for author isation of dong quai tincture from Angel ica sinensis (Oliv .) Diels as regards the use for all 
animal species excep t poultr y, horses dogs and cats, for author isation of asafoetida essential oil from Ferula-assa- 
foetida L. as regar ds the use for all animal species excep t dogs and cats and for author isation of dill herb essential oil 
from Anethum graveolens L. as regards all animal species excep t dogs and cats.
(1)OJ L 268, 18.10.2003, p. 29.
(2)Council Directive 70/524/EEC of 23 November 1970 concer ning additives in feedingstuffs (OJ L 270, 14.12.1970, p. 1).
Offi cial Jour nal  
of the European Union EN 
L series    
2024/260  15.1.2024ELI: http://data.europa.eu/eli/reg_im pl/2024/260/oj 2/11(4) The applicant requested the additives to be author ised for use also in wate r for drinking. In addition, for dong quai 
tincture from Angel ica sinensis (Oliv .) Diels the request for author isation for poultr y was only for a use in water for 
drinking. However , Regulation (EC) No 1831/2003 does not allow the author isation of ‘flavouri ng compo unds ’ for 
use in water for drinking. Theref ore, the use of those additives in wate r for drinking should not be allowed.
(5) The European Food Safety Author ity (‘the Author ity’) concluded in its opinions of 22 November 2022 (3)that, under 
the proposed conditions of use, cumin essential oil from Cuminum cyminum L., sweet fennel tincture from Foeniculum 
vulgare Mill. ssp. vulgare var. dulce, dong quai tincture from Ange lica sinensis (Oliv .) Diels, parsley tincture from 
Petroselinum crispum (Mill.) Fuss, star anise tincture from Illicium verum Hook f., dill herb essential oil from Anethum 
graveolens L. and dill tincture from Anethum graveolens L., are safe for the target species, consumers and the 
environment. As regar ds asafoetida essential oil from Ferula-assa-f oetida L., the Author ity considered that the 
substance is safe for dogs and cats and that there is no evidence of concer n for the envi ronment. The Auth ority also 
concluded that dill herb essential oil, cumin essential oil, dill tincture, sweet fennel tincture, asafoetida oil, dong quai 
tincture, parsle y tincture and star anise tincture should be considered as irritant to skin and eyes, and as dermal and 
respirato ry sensitisers. The Author ity concluded that when handling the cumin essential oil, dill herb essential oil, 
dill tincture, sweet fennel tincture and star anise tincture, exposure of unpro tected users to estragole (and dillapiole) 
cannot be excluded and that theref ore, to reduce the risk, the exposure of the users should be minimised. In 
addition, the Authority concluded that when handling parsley tincture, the exposure of unp rotect ed users to apiole, 
elemicin and myristicin cannot be excl uded and that theref ore, to reduce the risk, the exposure of the users should 
be minimised. The Authority further concluded that since the substances are recognised to flavour food and since 
their function in feed would be essentially the same as that in food, no further demonstration of efficacy is 
considered necessar y. It also verified the repor t on the method of analysis of the feed additives in feed submitted by 
the Reference Laboratory set up by Regulation (EC) No 1831/2003.
(6) In view of the abov e, the Commission considers that cumin essential oil from Cuminum cyminum L., sweet fennel 
tincture from Foeniculum vulgare Mill. ssp. vulgare var. dulce, dong quai tincture from Angel ica sinensis (Oliv .) Diels, 
parsle y tincture from Petroselinum crispum (Mill.) Fuss, star anise tincture from Illicium verum Hook f., asafoetida oil 
from Ferula-assa-f oetida L., dill herb essential oil from Anethum graveole ns L. and dill tincture from Anethum graveolens 
L. satisfy the conditions provided for in Article 5 of Regulation (EC) No 1831/2003 for certain animal species. 
According ly, the use of these substances should be author ised for those animal species. The Commission considers 
that safet y reasons do not require the setting of maximum cont ents for asafoetida essential oil from Ferula-assa- 
foetida L. and for dill herb essential oil from Anethum graveolens L.. The large margin of exposure and the lack of 
substances of concer n permit to establish maximum recommended contents. In order to allow bette r control, the 
recommended maximum content should be indicated on the label of the feed additives. Where such cont ents are 
exceed ed, certain information should be indicated on the label of the premixtures concer ned. For cumin essential 
oil from Cuminum cyminum L., sweet fennel tincture from Foeniculum vulgare Mill. ssp. vulgare var. dulce, parsle y 
tincture from Petroselinum crispum (Mill.) Fuss, star anise tincture from Illicium verum Hook f., dong quai tincture 
from Ang elica sinensis (Oliv .) Diels and dill tincture from Anethum graveole ns L., the Commission considers that the 
presence of substances of concer n requires to set a maximum conte nt in compl ete feed and to limit the 
combination of these additives with others containing the same substances of concer n. In addition, the Commission 
considers that appropr iate prot ective measures should be taken to prevent adverse effects on the health of the users 
of the additives.
(7) Since safet y reasons do not require the immediate application of the modifications to the conditions of author isation 
of the substances concer ned, it is appropr iate to provid e for a transitional period for interested parties to prepare 
themselves to meet the new requirements resulting from the author isation.
(8) The measures provid ed for in this Regulation are in accordance with the opinion of the Standing Committ ee on 
Plants, Animals, Food and Feed,
(3)EFSA Journal 2022;20(12):7690, EFSA Journal 2023;21(1):7691, EFSA Journal 2022;20(12):7692, EFSA Journal 2023;21(1):7693, 
EFSA Journal 2023;21(1):7694, EFSA Journal 2022;20(12):7695, EFSA Journal 2022;20(12):7689, EFSA Journal 2022;20(12):7688.OJ L, 15.1.2024 EN  ELI: http://data.europa.eu/eli/reg_impl/2024/260/oj 3/11HAS ADOPTED THIS REGUL ATION:
Article 1
Author isation
The substances specifi ed in the Annex, belonging to the additive cate gory ‘sensor y additives ’ and to the functional group 
‘flavouring compounds’, are author ised as additives in animal nutr ition, subject to the conditions laid down in that Annex.
Article 2
Transitional measures
1. The substances specified in the Annex and premixtures containing those substances, which are produced and labelled 
before 4 August 2024 in accordance with the rules applicable before 4 Febr uary 2024 may continue to be placed on the 
mark et and used until the existing stoc ks are exhaust ed.
2. Com pound feed and feed mater ials containing the substances specified in the Annex which are produced and labelled 
before 4 Febr uary 2025 in accordance with the rules applicable before 4 Febr uary 2024 may continue to be placed on the 
mark et and used until the existing stoc ks are exhaust ed if they are intended for food-producing animals.
3. Com pound feed and feed mater ials containing the substances specified in the Annex which are produced and labelled 
before 4 Febr uary 2026 in accordance with the rules applicable before 4 Febr uary 2024 may continue to be placed on the 
mark et and used until the existing stoc ks are exhaust ed if they are intended for non-f ood producing animals.
Article 3
Entr y into force
This Regulation shall enter into force on the twentieth day followi ng that of its publication in the Official Journal of the 
European Union.
This Regulation shall be binding in its entirety and directly applicable in all Member States.
Done at Brussels, 12 Januar y 2024 .
For the Commission
The President
Ursula VON DER LEYENOJ L, 15.1.2024 EN  ELI: http://data.europa.eu/eli/reg_im pl/2024/260/oj 4/11ANNEX 
Identification 
number of the 
additiveAdditiveComposition, chemical formula, descr iption, analytical 
method.Species or 
categ ory of 
animalMaximum 
ageMinimum 
conten tMaximum 
conten t
Other provisionsEnd of period 
of 
author isationmg /kg of active 
substance complet e feed 
with a moisture content 
of 12 %
Categor y: Sensor y additiv es. Functional group: Flavour ing compounds
2b161-eo Cumin 
essential oilAdditive composition
Cumin essential oil obtained from fruits of Cuminum 
cyminum L.
Liquid form
Char acter isation of the active substance
Cumin essential oil obtained by steam distillation of 
fruits of Cuminum cyminum L. as defined by the 
Council of Europe (1).
Specif ications
— Cuminaldehyde (4-isoprop ylbenzaldehy de): 
15-46 %
— γ-Terpinene: 13-32 %
— β-Pinene (pin-2(10)-ene):7-20 %
— α-Pinene (pin-2(3)-ene) :0,3-2 %
— Estragole: maximum 0,05 %
— Dillapiole: maximum 0,05 %
CAS number: 8014-13-9
FEMA number: 2343
CoE number: 161
Analytical method (2)
For the determination of alpha-pinene and 
cuminaldeh yde (phy tochemical markers) in the 
feed additive: gas chromat ography coupled to 
flame ionisation detection (GC-FID)All animal 
species- - 15 1.The additive shall be incor porated 
into the feed in the form of a premix ­
ture.
2.In the directions for use of the addi ­
tive and premixtures, the stora ge con­
ditions and stability to heat treatment 
shall be indicate d.
3.Mixture of cumin essential oil with 
other botanical additives is permitted 
provid ed that the amounts of estra ­
gole and dillapiole in feed mater ials 
and compo und feed are lower than 
the one resulting from the use of a 
sing le additive at the maximum or 
recommended cont ent for the species 
or animal cate gory.
4.For users of the additive and premix ­
tures, feed business operators shall 
establish operational procedures and 
organisational measures to address 
the poten tial risks resulting from 
their use. Where those risks cannot 
be eliminated by such procedures 
and measures, the additive and pre­
mixtures shall be used with personal 
breathing, eye and skin prot ective 
equipment.4.2.2034
(1)Natural sources of flavourings – Repor t No 2 (2007).
(2)Details of the analytical methods are available at the following address of the Reference Laborato ry: https://joint-research-c entre.ec.europa.eu/eurl-fa-eurl-f eed-additives/eurl-fa-author isation/eurl-f a-evaluation- 
repor ts_en .OJ L, 15.1.2024 EN  ELI: http://data.europa.eu/eli/reg_impl/2024/260/oj 5/11Identification 
number of the 
additiveAdditiveComposition, chemical formula, descr iption, analytical 
method.Species or 
categ ory of 
animalMaxi-mum 
ageMinimum 
conten tMaximum 
conten t
Other provisionsEnd of period 
of author i- 
sationmg /kg of active 
substance complet e feed 
with a moisture content 
of 12 %
Categor y: Sensor y additiv es. Functional group: Flavour ing compounds
2b200-t Sweet fennel 
tinctureAdditive composition
Sweet fennel tincture obtained from the dried fruit of 
Foeniculum vulgare Mill.
ssp. vulgare var. dulce
Liquid form
Char acter isation of the active substance
Sweet fennel tincture obtained from. the dried fruit 
of Foeniculum vulgare Mill.
ssp. vulgare var. dulce by extended extraction with 
water /ethanol solvent as defined by the Council of 
Europe (1).
Specif ications
Dry matt er content: maximum 3 %
— Total polyphenols: 0,05-0,07 %
— Flavonoids: 0,004-0,007 %
— Anisaldehyde : 0,003-0,005 %
— Anethole: 0,0005-0,0007 %
— Estragole: maximum 0,0006 %
FEMA number: 2482
CoE number: 200
Analytical method (2)
For the charact erisation of the feed additive:
— spectrophotometr y for the deter mination of 
total polyphenols, and
high performa nce thin-la yer chromat ography 
(HPTLC) for the determination of total flavo­
noids and the phytochemical marker anisalde ­
hydeAll animal 
species 
excep t 
horses
Horses- - 50
2001.The additive shall be incor porated 
into the feed in the form of a premix ­
ture.
2.In the directions for use of the addi ­
tive and premixtures, the stora ge con­
ditions and stability to heat treatment 
shall be indicate d.
3.Mixture of sweet fennel tincture with 
other botanical additives is permitted 
provid ed that the amounts of estra ­
gole in feed mater ials and compo und 
feed are lower than the one resulting 
from the use of a sing le additive at the 
maximum or recommended conte nt 
for the species or animal categor y.
4.For users of the additive and premix ­
tures, feed business operators shall 
establish operational procedures and 
organisational measures to address 
the poten tial risks resulting from 
their use. Where those risks cannot 
be eliminated by such procedures 
and measures, the additive and pre­
mixtures shall be used with personal 
breathing, eye and skin prot ective 
equipment.4.2.2034
(1)Natural sources of flavourings – Repor t No 2 (2007).
(2)Details of the analytical methods are available at the following address of the Reference Laborato ry: https://joint-research-c entre.ec.europa.eu/eurl-fa-eurl-f eed-additives/eurl-fa-author isation/eurl-f a-evaluation- 
repor ts_en .OJ L, 15.1.2024 EN  ELI: http://data.europa.eu/eli/reg_im pl/2024/260/oj 6/11
Identification 
number of the 
additiveAdditiveComposition, chemical formula, descr iption, analytical 
method.Species or 
categ ory of 
animalMaxi-mum 
ageMinimum 
conten tMaximum 
conten t
Other provisionsEnd of period 
of author i- 
sationmg active substance /kg 
of complet e feed with a 
moisture content of 
12 %
Categor y: Sensor y additiv es. Functional group: Flavour ing compounds
2b92456-t Dong quai 
tinctureAdditive composition
Dong quai tincture obtained from the roots of 
Angel ica sinensis (Oliv .) Diels
Liquid form
Char acter isation of the active substance
Dong quai tincture obtained from the roots of 
Angel ica sinensis (Oliv .) Diels by extraction with 
water /ethanol solvent as defined by the Council of 
Europe (1).
Specif ications
Dry matt er content: maximum 10 %
— Ferulic acid: 0,004-0,007 %
— Chlorogeni c acid: 0,001-0,006 %
— Safrole: maximum 0,0001 %
— Furocoumar ins: maximum 0,0001 %
Analytical method (2)
For the determination of ferulic acid and chlorogenic 
acid (phyt ochemical mark ers) in the feed additive: 
high performa nce liquid chromat ography coupled 
with diode array detection (HPL C-D AD)Horses
Dogs
Cats- - 123
481
1841.The additive shall be incor porated 
into the feed in the form of a premix ­
ture.
2.In the directions for use of the addi ­
tive and premixtures, the stora ge con­
ditions and stability to heat treatment 
shall be indicate d.
3.Mixture of dong quai tincture with 
other botanical additives is permitted 
provid ed that the amounts of furo ­
coumar ins and safrole in feed mater i­
als and compo und feed are lower 
than the one resulting from the use 
of a sing le additive at the maximum 
or recommended conte nt for the spe­
cies or animal categor y.
4.For users of the additive and premix ­
tures, feed business operators shall 
establish operational procedures and 
organisational measures to address 
the poten tial risks resulting from 
their use. Where those risks cannot 
be eliminated by such procedures 
and measures, the additive and pre­
mixtures shall be used with personal 
breathing, eye and skin prot ective 
equipment.4.2.2034
(1)Natural sources of flavourings – Repor t No 2 (2007).
(2)Details of the analytical methods are available at the following address of the Reference Laborato ry: https://joint-research-c entre.ec.europa.eu/eurl-fa-eurl-f eed-additives/eurl-fa-author isation/eurl-f a-evaluation- 
repor ts_en .OJ L, 15.1.2024 EN  ELI: http://data.europa.eu/eli/reg_impl/2024/260/oj 7/11Identification 
number of the 
additiveAdditiveComposition, chemical formula, descr iption, analytical 
method.Species or 
categ ory of 
animalMaxi-mum 
ageMinimum 
conten tMaximum 
conten t
Other provisionsEnd of period 
of author i- 
sationmg active substance /kg 
of complet e feed with a 
moisture content of 
12 %
Categor y: Sensor y additiv es. Functional group: Flavour ing compounds
2b2835-t Parsley 
tinctureAdditive composition
Parsley tincture obtained from the seeds of 
Petroselinum crispum (Mill.) Fuss
Liquid form
Char acter isation of the active substance
Parsley tincture obtained from the seeds of 
Petroselinum crispum (Mill.) Fuss by extended 
extraction with water /ethanol solvent as defined by 
the Council of Europe (1).
Specif ications
Dry matt er content: maximum 0,9 %
— Total polyphenols: 0,018-0,022 %
— Total flavonoids: 0,004-0,012 %
— Apiole: maximum 0,0092 %
— Elemicin: maximum 0,0016 %
— Myr isticin: maximum 0,0011 %
FEMA number:283 5(2)
Analytical method (3)
For the charact erisation of the feed additive:
— spectrophotometr y for the deter mination of 
total polyphenols and
— high performa nce thin-la yer chromat ography 
(HPTLC) for the determination of total flavo­
noids, myristicin and apiole .All animal 
species 
excep t 
horses
Horses- - 50
2001.The additive shall be incor porated 
into the feed in the form of a premix ­
ture.
2.In the directions for use of the addi ­
tive and premixtures, the stora ge con­
ditions and stability to heat treatment 
shall be indicate d.
3.Mixture of parsle y tincture with other 
botanical additives is permitted pro­
vided that the amounts of apiole, ele­
micin and myristicin in feed mat erials 
and compo und feed are lower than 
the one resulting from the use of a 
sing le additive at the maximum or 
recommended cont ent for the species 
or animal cate gory.
4.For users of the additive and premix ­
tures, feed business operators shall 
establish operational procedures and 
organisational measures to address 
the poten tial risks resulting from 
their use. Where those risks cannot 
be eliminated by such procedures 
and measures, the additive and pre­
mixtures shall be used with personal 
breathing, eye and skin prot ective 
equipment.4.2.2034
(1)Natural sources of flavourings – Repor t No 2 (2007).
(2)This number corresponds to parsley .
(3)Details of the analytical methods are available at the following address of the Reference Laborato ry: https://joint-research-c entre.ec.europa.eu/eurl-fa-eurl-f eed-additives/eurl-fa-author isation/eurl-f a-evaluation- 
repor ts_en .OJ L, 15.1.2024 EN  ELI: http://data.europa.eu/eli/reg_im pl/2024/260/oj 8/11
Identification 
number of the 
additiveAdditiveComposition, chemical formula, descr iption, analytical 
method.Species or 
categ ory of 
animalMaxi-mum 
ageMinimum 
conten tMaximum 
conten t
Other provisionsEnd of period 
of author i- 
sationmg active substance /kg 
of complet e feed with a 
moisture content of 
12 %
Categor y: Sensor y additiv es. Functional group: Flavour ing compounds
2b2095-t Star anise 
tinctureAdditive composition
Star anise tincture obtained from the fruit (per icarp 
and seeds) of Illicium verum Hook
f.
Liquid form
Char acter isation of the active substance
Star anise tincture obtained from the fruit (per icarp 
and seeds) of Illicium verum Hook
f. by exte nded extraction with water /ethanol solvent 
as defined by the Council of Europe (1)
Specif ications
Dry matt er content: maximum 2,2 %
— Total polyphenols: 0,21-0,31 %
— Total flavonoids: 0,022-0,024 %
— Rutin: 0,0024–0,0044 %
— Anethole: 0,0172–0,0182 %
— Estragole: maximum 0,00039 %
— Safrole: maximum 0,0010 %
FEMA number:209 5(2)
Analytical method (3)
For the charact erisation of the feed additive:
— spectrophotometr y for the deter mination of 
total polyphenols and
— high performa nce thin-la yer chromat ography 
(HPTLC) for the determination of total flavo­
noids and anetholeAll animal 
species 
excep t 
horses
Horses- - 50
200— The additive shall be incor porat ed 
into the feed in the form of a premix ­
ture.
—In the directions for use of the addi ­
tive and premixtures, the storage 
conditions and stability to heat treat ­
ment shall be indicated.
— Mixture of star anise tincture with 
other botanical additives is permitted 
provid ed that the amounts of estra ­
gole and safrole in feed mater ials and 
compo und feed are lower than the 
one resulting from the use of a sing le 
additive at the maximum or recom ­
mended content for the species or 
animal categor y.
—For users of the additive and premix ­
tures, feed business operators shall 
establish operational procedures 
and organisational measures to 
address the pote ntial risks resulting 
from their use. Where those risks 
cannot be eliminated by such proce ­
dures and measures, the additive and 
premixtures shall be used with perso ­
nal breathing, eye and skin prot ective 
equipment.4.2.2034
(1)Natural sources of flavourings – Repor t No 2 (2007).
(2)This number corresponds to star anise.
(3)Details of the analytical methods are available at the following address of the Reference Laborato ry: https://joint-research-c entre.ec.europa.eu/eurl-fa-eurl-f eed-additives/eurl-fa-author isation/eurl-f a-evaluation- 
repor ts_en .OJ L, 15.1.2024 EN  ELI: http://data.europa.eu/eli/reg_impl/2024/260/oj 9/11Identification 
number of the 
additiveAdditiveCompo sition, chem ical formula, descr iption, 
analytical method.Species or 
categ ory of 
animalMaximum 
ageMinimum 
conten tMaximum 
conten t
Other provisionsEnd of period 
of 
author isationmg active substance/kg 
of complet e feed with a 
moisture content of 
12 %
Categor y: Sensor y additiv es. Functional group: Flavour ing compounds
2b196-eo Asaf oetida 
essential oilAdditive composition
Asaf oetida essential oil obtained from the 
rhizome of Ferula-assa-f oetida L.
Liquid form
Char acter isation of the active substance
Asaf oetida essential oil obtained by steam 
distillation of dried gum resin of the cut 
rhizome of Ferula assa-foetida L. as defined 
by the Council of Europe (1).
Specif ications
— (E)-2-Butyl 3-(meth ylthio)-2-propenyl 
disulfi de: 20-45 %
— (E)-sec-Butyl propenyl disulfi de: 8-25 %
— (Z)-sec-Butyl propenyl disulf ide: 8-24 %
— Di-sec-Butyl disulfide: 4-16 %
CAS number: 9000-04-8
FEMA number: 2108
CoE number: 196
Analytical method (2)
For the determi nation of (E)-sec-butyl 
propeny l disulfi de (phyt ochemical mark er) 
in the feed additive: gas chromat ography 
coupled to flame ionisation detection 
(GC-FID)Cats
Dogs- - - 1.The additive shall be incor porat ed into the 
feed in the form of a premixture.
2.In the directions for use of the additive and 
premixtures, the storage conditions and sta­
bility to heat treatment shall be indicated.
3.On the label of the additive, the following 
shall be indicate d:
“Recommended maximum content of the ac­
tive substance per kg of compl ete feeding ­
stuff with a moisture content of 12 %:
— 1,5 mg for dogs
— 0,2 mg for cats”
4.The functional group, the identification 
number , the name and the added amount of 
the active substance shall be indicate d on the 
label of the premixture where the use level on 
the label of the premixture would result in 
exceed ing the level refer red to in point 3.
— For users of the additive and premixtures, 
feed business operators shall establish 
operational procedures and organisa ­
tional measures to address the poten tial 
risks resulting from their use. Where 
those risks cannot be eliminate d by 
such procedures and measures, the addi ­
tive and premixtures shall be used with 
personal breathing, eye and skin prote c­
tive equipment.4.2.2034
(1)Natural sources of flavourings – Repor t No 2 (2007).
(2)Details of the analytical methods are available at the following address of the Reference Laborato ry: https://joint-research-c entre.ec.europa.eu/eurl-fa-eurl-f eed-additives/eurl-fa-author isation/eurl-f a-evaluation- 
repor ts_en .OJ L, 15.1.2024 EN  ELI: http://data.europa.eu/eli/reg_im pl/2024/260/oj 10/11
Identification 
number of the 
additiveAdditiveCompo sition, chem ical formula, descr iption, 
analytical method.Species or 
categ ory of 
animalMaxi-mum 
ageMinimum 
conten tMaximum 
conten t
Other provisionsEnd of period 
of author i- 
sationmg active substance /kg 
of complet e feed with a 
moisture content of 
12 %
Categor y: Sensor y additiv es. Functional group: Flavour ing compounds
2b42-eo Dill herb 
essential oilAdditive composition
Dill herb essential oil obtained from stems 
and leav es of Anethum graveolens L.
Liquid form
Char acter isation of the active substance
Dill herb essential oil obtained from stems 
and leav es of Anethum graveolens L. by 
steam distillation as defined by the Council 
of Europe (1).
Specif ications
— Carvone: 28-45 %
— Limonene: 16-35 %
— α-Phellandrene: 16-31 %
— Dill ether (3,6-Dimethy l-2,3,3a,4,5,7a- 
hexahydroben zofuran): 4-9 %
— Estragole: maximum 0,0003 %
— Myr isticin: maximum 0,005 %
— Apiole: maximum 0,0003 %
— Dillapiole: maximum 0,01 %
CAS number:800 6-75-5
FEMA number: 2383
CoE number: 42
Analytical method (2)
For the deter mination of carvone 
(phyt ochemical marker) in the feed 
additive: gas chromat ography coupled to 
flame ionisation detection (GC-FID)Dogs
Cats- - - 1.The additive shall be incor porat ed into the 
feed in the form of a premixture.
2.In the directions for use of the additive and 
premixtures, the storage conditions and sta­
bility to heat treatment shall be indicated.
3.On the label of the additive, the following 
shall be indicate d:
“Recommended maximum content of the ac­
tive substance per kg of compl ete feeding ­
stuff with a moisture content of 12 %:
— 7 mg for dogs
— 5 mg for cats”
4.The functional group, the identification 
number , the name and the added amount of 
the active substance shall be indicate d on the 
label of the premixture where the use level on 
the label of the premixture would result in 
exceed ing the level refer red to in point 3.
5.For users of the additive and premixtures, 
feed business operat ors shall establish opera ­
tional procedures and organisational mea ­
sures to address the poten tial risks resulting 
from their use. Where those risks cannot be 
eliminated by such procedures and measures, 
the additive and premixtures shall be used 
with personal breathing, eye and skin prote c­
tive equipment.4.2.2034
(1)Natural sources of flavourings – Repor t No 2 (2007).
(2)Details of the analytical methods are available at the following address of the Reference Laborato ry: https://joint-research-c entre.ec.europa.eu/eurl-fa-eurl-f eed-additives/eurl-fa-author isation/eurl-f a-evaluation- 
repor ts_en .OJ L, 15.1.2024 EN  ELI: http://data.europa.eu/eli/reg_impl/2024/260/oj 11/11Identification 
number of the 
additiveAdditiveComposition, chemical formula, descr iption, analytical 
method.Species or 
categ ory of 
animalMaxi-mum 
ageMinimum 
conten tMaximum 
conten t
Other provisionsEnd of period 
of author i- 
sationmg active substance /kg 
of complet e feed with a 
moisture content of 
12 %
Categor y: Sensor y additiv es. Functional group: Flavour ing compounds
2b42-t Dill tincture Additive composition
Dill tincture obtained from the fruit of Anethum 
graveolens L.
Liquid form
Char acter isation of the active substance
Dill tincture obtained from the dried fruit of 
Anethum graveolens L. by exte nded extraction with a
water /ethanol solvent as defined by the Council of 
Europe (1).
Specif ications
Dry matt er content: maximum 1,1 %
— Total polyphenols: 0,02-0,03 %
— Flavonoids:0,01-0,02 %
— Carvone: 0,001-0,005 %
— Estragole: maximum 1,2 mg/kg
FEMA number: 2382
CoE number: 42
Analytical method (2)
For the character isation of the feed additive (dill 
tincture):
— spectrophotometr y for the deter mination of 
total polyphenols and
— high performa nce thin-la yer chromat ography 
(HPTLC) for the determination of total flavo­
noids and the phytochemical mark er carvoneAll animal 
species 
excep t 
horses
Horses- - 50
2001.The additive shall be incor porated 
into the feed in the form of a premix ­
ture.
2.In the directions for use of the addi ­
tive and premixtures, the stora ge con­
ditions and stability to heat treatment 
shall be indicate d.
3.Mixture of dill tincture with other bo­
tanical additives is permitted pro­
vided that the amounts of estragole 
in feed mater ials and compo und feed 
are lowe r than the one resulting from 
the use of a sing le additive at the max ­
imum or recommended level for the 
species or animal catego ry.
4.For users of the additive and premix ­
tures, feed business operators shall 
establish operational procedures and 
organisational measures to address 
the poten tial risks resulting from 
their use. Where those risks cannot 
be eliminated by such procedures 
and measures, the additive and pre­
mixtures shall be used with personal 
breathing, eye and skin prot ective 
equipment.4.2.2034
(1)Natural sources of flavourings – Repor t No 2 (2007).
(2)Details of the analytical methods are available at the following address of the Reference Laborato ry: https://joint-research-c entre.ec.europa.eu/eurl-fa-eurl-f eed-additives/eurl-fa-author isation/eurl-f a-evaluation- 
repor ts_en .OJ L, 15.1.2024 EN