1 
 ANNEX  I 
 
Identifi -
cation 
number 
of the 
additive  Additive  Composition, chemical formula, 
description, analytical method.  Species or 
category of 
animal  Maxi -
mum 
age Minimum 
content  Maximum 
content  
Other provisions  End of 
period of 
authori -
sation  mg active substance /kg 
of complete feed with a 
moisture content of 
12%  
Category: Sensory additives. Functional group: Flavouring compounds  
2b143 -
eo Expressed 
orange essential 
oil Additive composition  
Expressed orange essential oil from 
fruit peels of Citrus sinensis  (L.) 
Osbeck  (1). 
Liquid form  
 
Characterisation of the active 
substance  
Essential oil derived by cold 
expression from fruit peel of Citrus 
sinensis  (L.) Osbeck as defined by 
the Council of Europe  (2). 
 
In the volatile fraction:  
 
d-Limonene: 93 -97% 
Myrcene: 1. 5-3.5% 
Sabinene: 0. 1-1.0% 
α-Pinene: 0. 4-0.8% 
Linalool: 0 .1-0.7%  
Decanal: 0.1 -0.7%  Chickens for 
fattening and 
minor 
poultry 
species for 
fattening  
 
Laying hens 
and minor 
poultry 
species for 
laying and 
breeding  
 
Turkeys for 
fattening  - - 80 1. The additive shall be 
incorporated into the feed in the 
form of a premixture.  
2. In the directions for use of the 
additive and premixtures, the 
storage conditions and stability 
to heat treatment shall be 
indicated.   
3. Mixture with other botanical 
additives is permitted provided 
that the amounts of 
perillaldehyde  added to 
feedingstuffs  by such mixtures 
is lower than the one resulting 
from the use of a single additive 
at the maximum or 
recommended level for the 
specie s or category  of animal.  
4. For users of the additive and 
premixtures, feed business 
operators shall establish 
operational pro cedures and 
organisational measures to 
address potential risks by 12 
September 
2032  
All Suidae  
species for 
fattening  - - 172 
Piglets of all 
Suidae 
species  - - 144 
                                                           
(1) Synonyms: Citrus sinensis (L.) Pers., Citrus aurantium (L.) Dulcis.  
(2) Natural sources of flavourings - Report No. 2 (2007) . 2 
 Octanal: 0.1 -0.6%  
 
 
Perillaldehyde: < 0.05%  
 
CAS number: 8028 -48-6 
EINECS number: 232-433-8 
FEMA number: 2825  
CoE number: 143  
 
Analytical method  (3) 
For the determination of d -limonene 
(phytochemical marker) in the feed 
additive:  
- gas chromatography coupled 
with flame ionisation detection 
(GC-FID) (based on ISO 3140)  Sows  of all 
Suidae 
species  - - 200 inhalation, dermal contact or 
eyes contact. Where those risks 
cannot be eliminated by such 
procedures and measures, the 
additive and premixtures shall 
be used with personal skin, eye 
and bre athing protective 
equipment.  Ruminants  - - 130 
Horses  - - 230 
Other animal 
species and 
categories 
except dogs, 
cats, 
ornamental 
fish and 
ornamental 
birds  - - 50 
 
 
  
                                                           
(3) Details of the analytical methods are available at the following address of the Reference Laboratory: https://ec.europa.eu/jrc/en/eurl/feed -additives/evaluation -reports  3 
 Identifi -
cation 
number 
of the 
additive  Additive  Composition, chemical formula, 
description, analytical method.  Species or 
category of 
animal  Maxi -
mum 
age Minimum 
content  Maximum 
content  
Other provisions  End of 
period of 
authori -
sation  mg active substance /kg 
of complete feed with a 
moisture content of 12%  
Category: Sensory additives. Functional group: Flavouring compounds  
2b143 -
di Distilled orange 
essential oil Additive composition  
Distillate (volatile fraction) of  orange 
essential oil, which is obtained from 
fruit peel of Citrus sinensis  (L.) 
Osbeck.  
Liquid form  
 
Characterisation of the active 
substance  
Volatile fraction from the distillation 
of orange essential oil expressed 
(cold pressed), which is obtained 
from the fruit peel of Citrus sinensis  
(L.) Osbeck  as defined by the 
Council of Europe  (4). 
 
d-Limonene: 93-97.5% 
Myrcene: 1.5 -3.5%  
Sabinene: 0.2 -1.0%  
α-Pinene: 0.3 -0.8%  
Linalool: 0.05 -0.5%  Chickens for 
fattening and 
minor poultry 
species for 
fattening  
 
Laying hens 
and minor 
poultry 
species for 
laying and 
breeding  
 
Turkeys for 
fattening  
 - 
 
 - 
 80 
 1. The additive shall be 
incorporated into the feed in the 
form of a premixture.  
2. In the directions for use of the 
additive and premixtures, the 
storage conditions and stability 
to heat treatment shall be 
indicated.   
3. Mixture with other botanical 
additives is permitted provided 
that the amounts of 
perillaldehyde  added to 
feedingstuffs  by such mixtures is 
lower than the one resulting from 
the use of a single additive at the 
maximum or recommended level 
for the species or category  of 
animals.  
4. For users of the additive and 
premixtures, feed business 
operators shall establish 
operational p rocedures and 
organisational measures to 12 
September 
2032  
Suidae   - - 200 
Ruminants  - - 130 
Horses  - - 225 
                                                           
(4) Natural sources of flavourings - Report No. 2 (2007).  4 
 Octanal:  0.05-0.4%  
 
Perillaldehyde: < 0.005%  
 
CAS number: 8028 -48-6 
CoE number : 143  
 
Analytical method  (5) 
For the determination  of d-limonene 
(phytochemical marker) in the feed 
additive:  
- gas chromatography coupled 
with flame ionisation detection 
(GC-FID) (based on ISO 3140)  Other animal 
species and 
categories  
except dogs, 
cats, 
ornamental 
fish and 
ornamental 
birds   - - 80 address potential risks by 
inhalation, dermal contact or 
eyes contact. Where those risks 
cannot be eliminated by such 
procedures and measures, the 
additive and premixtures shall be 
used with personal skin, eye and 
breathing protective equipment.  
 
  
                                                           
(5) Details of the analytical methods are available at the following address of the Reference Laboratory: https://ec.eu ropa.eu/jrc/en/eurl/feed -additives/evaluation -reports  5 
 Identifi -
cation 
number 
of the  
additive  Additive  Composition, chemical formula, 
description, analytical method.  Species or 
category of 
animal  Maxi -
mum 
age Minimum 
content  Maximum 
content  
Other provisions  End of 
period of 
authori -
sation  mg active substance /kg 
of complete feed with  a 
moisture content of 
12%  
Category: Sensory additives. Functional group: Flavouring compounds  
2b143 -f Folded orange 
oil  Additive composition  
Folded oil obtained from the fruit 
peel of Citrus sinensis  (L.) Osbeck.  
Liquid form  
 
Characterisation of the active 
substance  
Folded oil  produced by the fractional 
distillation of an expressed orange 
essential oil obtained from the fruit 
peel of Citrus sinensis  (L.) Osbeck  
as defined by the Council of Europe  
(6). 
 
Non-volatile fraction: 10.5%  
 
In the volatile fraction:  
 
d-Limonene: 89 -96% 
Decanal: 0.5 -2% 
Linalool: 0.7 -1.7%  
Myrcene: 0.1 -1.0%  
Geranial: 0.1 -1.0%  Chickens for 
fattening and 
minor poultry 
species for 
fattening  - - 15.5 1. The additive shall be 
incorporated into the feed in the 
form of a premixture.  
2. In the directions for use of the 
additive and premixtures, the 
storage conditions and stability 
to heat treatment shall be 
indicated.   
3. Mixture with other botanical 
additives is permitted provided 
that the amounts of 
perillaldehyde  added to 
feedingstuffs  by such mixtures is 
lower than the one resulting 
from the use of a single additive 
at the maximum or 
recommended level for the 
species or category of  animals.  
4. For users of the additive and 
premixtures, feed business 
operators shall establish 
operational procedures and 
organisational measures to 
address potential risks by 
inhalation, dermal contact or 
eyes contact. Where those risks 12 
September 
2032  
Laying hens 
and minor 
poultry 
species for 
laying and 
breeding  - - 23.5 
Turkeys for 
fattening  - - 21 
All Suidae  for 
fattening  - - 34 
Piglets of all 
Suidae 
species  - - 28.5 
Sows  of all 
Suidae 
species  - - 41.5 
                                                           
(6) Natural sources of flavourings - Report No. 2 (2007).  6 
 Perillaldehyde: < 0.3%  
 
CAS  number : 8028 -48-6 
FEMA  number : 282 2 
 
Analytical method  (7) 
For the determination  of d-limonene 
(phytochemical marker) in the feed 
additive:  
- gas chromatography coupled 
with flame ionisation detection 
(GC-FID) (based on ISO 3140)  Calves (milk 
replacer)  - - 66.5 cannot be eliminated by such 
procedures and measures, the 
additive and premixtures shall be 
used with personal skin, eye and 
breathing protective equipment.  Sheep and 
goats  
 
Other 
ruminants for 
fattening  - - 62.5 
Dairy 
ruminants  
other than 
dairy sheep 
and dairy 
goats  - - 40.5 
Horses  - - 62.5 
Rabbits  - - 25 
Fish except 
ornamental 
fish - - 70 
   Other animal  
species  and 
categories  
except dogs, 
cats, 
ornamental 
fish and 
ornamental 
birds  - - 15.5   
  
                                                           
(7) Details of the analytical methods are available at the following address of the Reference Laboratory: https://ec.europa.eu/jrc/en/eurl/feed -additives/evaluation -reports  7 
 Identifi -
cation 
number 
of the 
additive  Additive  Composition, chemical formula, 
description, analytical method.  Species or 
category of 
animal  Maxi -
mum 
age Minimum 
content  Maximum 
content  
Other provisions  End of 
period of 
authori -
sation  mg active substance  
/kg of complete feed 
with a moisture 
content of 12%  
Category: Sensory additives. Functional group: Flavouring compounds  
2b143 -
f-i Folded orange 
oil  Additive composition  
Folded oil obtained from the fruit 
peel of Citrus sinensis  (L.) Osbeck.  
Liquid form  
 
Characterisation of the active 
substance  
Folded oil  produced by the fractional 
distillation of an expressed orange 
essential oil obtained from the fruit 
peel of Citrus sinensis  (L.) Osbeck  
as defined by the Council of Europe  
(8). 
 
Non-volatile fraction: 20.9%  
 
In the volatile fraction:  
 
d-Limonene: 79 -89% 
Decanal: 3.0 -5.0%  
Linalool: 2.0 -5.0%  
Myrcene: 0.1 -1.0%  
Geranial: 0.5 -1.8%  Chickens for 
fattening and 
minor poultry 
species for 
fattening  - - 5.5 1. The additive shall be 
incorporated into the feed in the 
form of a premixture.  
2. In the directions for use of the 
additive and premixtures, the 
storage conditions and stability 
to heat treatment shall be 
indicated.   
3. Mixture with other botanical 
additives is permitted provided 
that the amounts of 
perillaldehyde  added to 
feedingstuffs  by such mixtures is 
lower than the one resulting 
from the use of a single additive 
at the maximum or 
recommended level for the 
species or  category  of animals . 
4. For users of the additive and 
premixtures, feed business 
operators shall establish 
operational procedures and 
organisational measures to 
address potential risks by 
inhalation, dermal contact or 
eyes contact. Where those risks 
cannot b e eliminated by such 12 
September 
2032  
Laying hens 
and minor 
poultry 
species for 
laying and 
breeding  - - 8 
Turkeys for  
fattening  - - 7 
All Suidae  for 
fattening  - - 11.5 
Piglets of all 
Suidae  
species  - - 9.5 
Sows  of all 
Suidae 
species  - - 14 
                                                           
(8) Natural sources of flavourings - Report No. 2 (2007).  8 
  
Perillaldehyde: < 0.6%  
 
CAS number : 8028 -48-6 
FEMA  number : 282 2 
 
Analytical method  (9) 
For the determination  of d-limonene 
(phytochemical marker) in the feed 
additive:  
- gas chromatography coupled 
with flame ionisation detection 
(GC-FID) (based on ISO 11024 ) Calves (milk 
replacer)  - - 23 procedures and measures, the 
additive and premixtures shall 
be used with personal skin, eye 
and breathing protective 
equipment.  Sheep and 
goats  
 
Other 
ruminants for 
fattening  - - 21.5 
Dairy 
ruminants  
other than 
dairy sheep 
and dairy 
goats  - - 14 
Horses  - - 21.5 
Rabbits  - - 8.5 
Fish except 
ornamental 
fish - - 24.5 
   Other animal  
species  and 
categories  
except dogs, 
cats, 
ornamental 
fish and 
ornamental 
birds  - - 5.5   
 
  
                                                           
(9) Details of the analytical methods are available at the following address of the Reference Laboratory: https://ec.europa.eu/jrc/en/eurl/feed -additives/evaluation -reports  9 
  
 
Identifi -
cation 
number 
of the 
additive  Additive  Composition, chemical formula, 
description, analytical method.  Species or 
category of 
animal  Maxi -
mum 
age Minimum 
content  Maximum 
content  
Other provisions  End of 
period of 
authori -
sation  mg active substance /kg 
of complete feed with a 
moisture content of 
12%  
Category: Sensory additives. Functional group: Flavouring compounds  
2b143 -
f-ii Folded orange 
oil  Additive composition  
Folded oil obtained from the fruit 
peel of Citrus sinensis  (L.) Osbeck.  
Liquid form  
 
Characterisation of the active 
substance  
Folded oil  produced by the fractional 
distillation of an expressed orange 
essential oil which is obtained from 
the fruit peel of Citrus sinensis  (L.) 
Osbeck  as defined by the Council of 
Europe  (10). 
 
Non-volatile fraction: 18%  
 
In the volatile fraction:  
d-Limonene: 85-95% 
Linalool: 0.5-4% 
 
CAS  number : 8028 -48-6 
FEMA  number : 282 2 All animal 
species  
except horses, 
rabbits, dogs , 
cats, 
ornamental 
fish and 
ornamental 
birds  - - - 1. The additive shall be incorporated 
into the feed in the form of a 
premixture.  
2. In the directions for use of the 
additive and premixtures, the 
storage conditions and stability to 
heat treatment shall be indicated.  
3. On the label of the additive the 
following shall be indicated:  
“Recommended maximum 
content of the active substance per 
kg of complete feedingstuff with a 
moisture content of 12%:  
 Chickens for fattening and 
minor poultry species for 
fattening , laying hens and 
minor poultry species for 
laying and breeding , turkeys 
for fattening, Suidae : 50 mg;  
 Calves (milk replacer) , sheep, 
goats : 70 mg;  
 Ruminants except sheep and 
goats : 60 mg; 
 Other animal  species  and 
categories except  horses, 12 
September 
2032  
 
                                                           
(10) Natural sources of flavourings - Report No. 2 (2007).  10 
  
Analytical method  (11) 
For the determination  of d-limonene 
(phytochemical marker) in the feed 
additive:  
- gas chromatography coupled 
with flame ionisation detection 
(GC-FID) (based on ISO 3140)  rabbits, dogs, cats, ornamental 
fish and ornamental birds : 2 
mg”. 
4. The functional group, the 
identification number, the name 
and the added amount of the active 
substance shall be indicated on the 
label of the premixture where the 
use level on the label of the 
premixture would result in 
exceeding the level referred to in 
point 3.  
5. For users of the additive and 
premixtures, feed business 
operators shall establish 
operational procedures and 
organisational measures to address 
potential risks by inhalation, 
dermal contact or eyes contact. 
Where those risks cannot be 
eliminated b y such procedures and 
measures, the additive and 
premixtures shall be used with 
personal skin, eye and breathing 
protective equipment.  
 
 
 
  
                                                           
(11) Details of the analytical methods are available at the following address of the Reference Laboratory: https://ec. europa.eu/jrc/en/eurl/feed -additives/evaluation -reports  11 
 ANNEX  II 
 
Identifi -
cation 
number of 
the 
additive  Name of 
the holder 
of 
authorisa -
tion Additive  Composition, chemical formula, 
description, analytical method.  Species or 
category of 
animal  Maxi -
mum 
age Minimum 
content  Maximum 
content  
Other provisions  End of 
period of 
authori -
sation  mg active substance /kg 
of complete feed with a 
moisture content of 
12%  
Category: Sensory additives. Functional group: Flavouring compounds  
2b136-ex - Bitter 
orange 
extract   Additive composition  
Bitter orange extract from the 
fruit of Citrus x aurantium  L. 
Solid  form  
 
Characterisation of the active 
substance  
Bitter orange extract from the 
fruit of Citrus x aurantium  L. as 
defined by the Council of 
Europe  (12). 
 
Flavonoids : 45-55 % of which  
 Naringin: 20 -30% 
 Neohesperidin : 10-20% 
 
5-methoxypsoralen (also known 
as bergapten):  ≤ 0.03%  
(-)-Synephrine: ≤ 1%  
 
CoE number: 1 36 All animal 
species 
except 
dogs, cats , 
ornamental 
fish and 
ornamental 
birds  - -  1. The additive shall be 
incorporated into the feed in the 
form of a premixture.  
2. In the directions for use of the 
additive and premixtures, the 
storage conditions and stability 
to heat treatment shall be 
indicated.  
3. On the label of the additive the 
following shall be indicated:  
“Recommended maximum 
content of the active substance 
per kg of complete feedingstuff 
with a moisture content of 12%:  
 Chickens for fattening  and 
minor poultry species for 
fattening : 102 mg; 
 Laying hens  and minor 
poultry  species  for laying  
or breeding : 151 mg; 
 Turkeys for fattening: 13 6 
mg; 
 Piglets  of all  Suidae  
species : 182 mg ; 11 May 
2032  
                                                           
(12) Natural sources of flavourings - Report No. 2 (2007) . 12 
  
Analytical method  (13) 
For the quantification of 
naringin (phytochemical marker) 
in the feed additive:  
- High performance liquid 
chromatography (HPLC) 
with spectrophotometric 
(UV) detection   All Suidae  for fattening: 
217 mg; 
 Sows of all Suidae  species : 
268 mg; 
 Dairy ruminants  other than 
dairy sheep and dairy goats : 
259 mg; 
 Calves ( milk replacer) , 
sheep , goats, other 
ruminants for fattening, 
horse s, salmon ids and all 
other fin fish : 400 mg; 
 Rabbits: 1 61 mg; 
 Other animal species  and 
categories except dogs, 
cats, ornamental fish  and 
ornamental birds : 102 mg ”. 
4. The functional group, the 
identification number, the name 
and the added amount of the 
active substance shall be 
indicated on the label of the 
premixture where the use level 
on the label of the premixture 
would result in exce eding the 
level referred to in point 3.  
5. Mixture of bitter orange extract  
with other botanical additives is 
permitted provided that the 
amounts of 5 -methoxypsoralen 
and (-)-synephrine in feed 
materials and compound feed 
are lower than the one s resulting 
from the use of a single additive 
at the maximum or 
recommended level for the 
                                                           
(13) Details of the analytical methods are available at the following address of the Reference Laboratory: https://ec.europa.eu/jrc/en/eurl/feed -additives/evaluation -reports   13 
 species or animal category.  
6. The mixture of bitter orange 
extract from the fruit of Citrus x 
aurantium  L. with other 
authorised additives obtained 
from Citrus aurantium  L. shall 
not be allowed in feedingstuffs.  
7. For users of the additive and 
premixtures, feed business 
operators shall establish 
operational procedures and 
organisational measures to 
address potential risks by 
inhalation, dermal contact or 
eyes contact. Where  those risks 
cannot be eliminated by such 
procedures and measures, the 
additive and premixtures shall 
be used with personal skin, eye 
and breathing protective 
equipment.  
 
  14 
 ANNEX  III 
Identifi -
cation 
number 
of the 
additive  Name of the 
holder of  
authorisation  Additive  Composition, chemical formula, 
description, analytical method  Species or 
category of 
animal  Maxi -
mum 
age Mini -
mum 
content  Maxi -
mum 
content  
Other provisions  End of 
period of 
authorisatio
n mg active 
substance/kg of 
complete feed with a 
moisture content of 
12%  
Category: Sensory additives. Functional group: Flavouring compounds  
2b139 -eo - Expressed 
lemon 
essential oil  Additive composition  
Expressed lemon e ssential oil from fruit 
peel of Citrus limon  (L.) Osbeck  
Liquid form  
 
Characterisation of the active 
substance  
Expressed lemon e ssential oil obtained  
by cold expression from fruit peel of 
Citrus limon  (L.) Osbeck  as defined by 
the Council of Europe (14) 
d-Limonene: 60 –73 %  
β-Pinene (pin -2(10) -ene): 9 –18% 
γ-Terpinene : 6–12% 
α-Pinene (pin -2(3)-ene): 1.3 –3.0%  
Sabinene (4(10) -thujene): 0.3–3.0%  
Geranial: 0.1 –2.0%  
Neral: 0.1 –1.8%  
Perillaldehyde: ≤ 0.023%  
Furocoumarins: ≤ 0.3 %  
Methoxycoumarins: ≤ 0,06%  
 
CAS number 84929 -31-7 Chickens 
for fat tening 
and minor 
poultry 
species for 
fattening  
 - - 35 1. The additive shall be 
incorporated into the 
feed in the form of a 
premixture.  
2. In the directions for use 
of the additive and 
premixtures, the 
storage conditions and 
stability to heat 
treatment shall be 
indicated.  
3. Mixture of expressed 
lemon essential oil with 
other botanical 
additives is permitted 
provided that the 
amounts of 
perillaldehyde , 
furocoumarins, and 
methoxycoumarins in 
feed materials and 
compound feed are 
lower than the one 
resulting from the use 
of a single additive at 
the maximum or 29 
September 
2032  
Turkeys for 
fattening  
 
Salmonids 
and other fin 
fish except 
ornemental 
fish 40 
Laying hens  
and minor 
poultry  
species for 
laying or 
breeding  52 
All Suidae  
for fattening  74 
                                                           
(14)  Natural sources of flavourings - Report No. 2 (2007) . 15 
 FEMA number 2625  
CoE number: 139  
 
Analytical method (15) 
For the quantification of phytochemical 
marker d-limonene  in the feed additive :  
- Gas chromatography coupled with 
flame ionisation detection (GC -
FID) (based on  ISO 855)  
 
 Piglets  of all 
Suidae  
species  62 recommende d level for 
the species or animal 
category.  
4. For users of the 
additive and 
premixtures, feed 
business operators shall 
establish operational 
procedures and 
organisational 
measures to address 
potential risks by 
inhalation, dermal 
contact or eyes contact. 
Where those risks 
cannot be eliminated 
by such procedures and 
measures, the additive 
and premixtures shall 
be used with personal 
skin, eye and breathing 
protective equipment . Sows of all 
Suidae  
species  92 
Calves 
(milk 
replacers)  
 
Ruminants 
for fattening  
other than 
sheep and 
goats  
 
Dairy 
ruminants  
other than 
sheep and 
goats  90 
 
Horses  137 
Other 
animal  
species and 
categories 
except dogs, 
cats, 
ornamental 
fish and 
ornamental 
birds  30 
 
2b139 - rf - Residual 
fraction of 
expressed Additive composition  
Residual fraction of expressed lemon 
oil distilled  from fruit peel of Citrus Chickens 
for fattening  
and minor 
poultry - - 11 
 1. The additive shall be 
incorporated into the 
feed in the form of a 29 
September 
2032  
                                                           
(15) Details of the analytical methods are available at the following address of the Reference Laboratory: https://ec.europa.eu/jrc/en/eurl/feed -additives/evaluation -reports   16 
 lemon oil 
distilled  limon  (L.) Osbeck  
Liquid form  
 
Characterisation of the active 
substance  
Residual fraction of expressed lemon 
oil distilled  obtained by  the distillation 
of lemon essential oil expressed from 
fruit peel of Citrus limon  (L.) Osbeck  as 
defined by the Council of Europe (16) 
 
The specifications of the active 
substance are : 
d-Limonene: 51 –63 %  
γ-Terpinene: 8 –17%  
Geranial: 6 –12% 
Neral :5–9 % 
β-Pinene (pin -2(10) -ene): 0.3–5.5%  
β-Bisabolene:  0.3 –4% 
 
Perillaldehyde: ≤ 0.092%  
Furocoumarins: ≤ 0.8%  
Methoxycoumarins: ≤ 0.22%   
 
 
CoE number : 139  
 
Analytical method (17) 
For the quantification of phytochemical 
marker d-limonene  in the feed additive:  
- Gas chromatography coupled with species for 
fattening   
 premixture.  
2. In the directions for use 
of the additive and 
premixtures, the storage 
conditions and stability 
to heat treatment shall 
be indicated . 
3. Mixture of residual 
fraction of expressed 
lemon oil  distilled  with 
other botanical 
additives is permitted 
provided that the 
amounts of 
perillaldehyde , 
furocoumarins, and 
methoxycoumarins in 
feed materials and 
compound feed are 
lower than the one 
resulting from the use 
of a single additiv e at 
the maximum or 
recommended level for 
the species or animal 
category.  
4. For users of the additive 
and premixtures, feed 
business operators shall 
establish operational 
procedures and 
organisational measures 
to address potential 
risks by inhalation, Laying hens  
and minor 
poultry  
species for 
laying or 
breeding  
 
Turkeys for 
fatening  
 
Rabbits  
 
Salmonids  
and other fin 
fish except 
ornemental 
fish 12 
Ruminants  20 
Piglets  of all 
Suidae  
species  20 
All Suidae  
for fattening  24 
Sows  of all 
Suidae  
species  30 
                                                           
(16) Natural sources of flavourings - Report No. 2 (2007).  
(17) Details of the analytical methods are available at the following address of the Reference Laboratory: https://ec.europa.eu/jrc/en/eurl/feed -additives/evaluation -reports  17 
 flame ionisation detection (GC -
FID) (based on ISO 855)  
 
 Horses  35 dermal  contact or eyes 
contact.  Where those 
risks cannot be 
eliminated by such 
procedures and 
measures, the additive 
and premixtures shall 
be used with personal 
skin, eye and breathing 
protective equipment . 
Other 
animal 
species and 
categories 
except dogs, 
cats, 
ornamental 
fish and 
ornamental 
birds  11 
2b139 -di - Distilled 
lemon 
essential oil 
(volatile 
fraction)   
 Additive composition  
Distilled lemon essential oil (volatile 
fraction) obtained from  lemon essential 
oil expressed from fruit peel of Citrus 
limon  (L.) Osbeck  
Liquid form  
 
Characterisation of the active 
substance  
Distilled lemon essential oil (volatile 
fraction)  obtained by  lemon essential oil 
expressed from fruit peel of Citrus 
limon  (L.) Osbeck  as defined by the 
Council of Europe (18) 
The specifications of the active Chickens 
for fattening 
and minor 
poultry 
species for 
fattening  - - 36 1. The additive shall be 
incorporated into the 
feed in the form of a 
premixture.  
2. In the directions for use 
of the additive and 
premixtures, the storage 
conditions and stability 
to heat treatment shall 
be indicated.  
3. Mixture of distilled 
lemon essential oil 
(volatile fraction) with 
other botanical 
additives is permitted 
provided that the 
amounts of 
furocoumarins, and 29 
September 
2032  
Laying hens 
and minor 
poultry  
species for 
laying or 
breeding  53 
Rabbits  56 
Turkeys for 
fattening  48 
                                                           
(18) Natural sources of flavourings - Report No. 2 (2007).  18 
 substance are:  
d-Limonene: 66 –78 %  
β-Pinene (pin -2(10) -ene): 5–20%  
γ-Terpinene: 1.5 –9.5 %  
α-Pinene (pin -2(3)-ene): 0.5 –3.0% 
Sabinene:  0.3 –3.0%  
Furocoumarins: ≤ 0.1 mg/kg  
Methoxycoumarins: ≤ 0.1 mg/kg   
 
CoE number : 139  
 
Analytical method (19) 
For the quantification of phytochemical 
marker d-limonene  in the feed additive:  
- Gas chromatography coupled with 
flame ionisation detection (GC -
FID) (based on ISO 855)  
 Piglets  of all 
Suidae  
species  64 methoxycoumarins in 
feed materials and 
compound feed are 
lower than the one 
resulting from the use 
of a single additive at 
the maximum or 
recomm ended level for 
the species or animal 
category.  
4. For users of the additive  
and premixtures, feed 
business operators shall 
establish operational 
procedures and 
organisational measures 
to address potential 
risks by inhalation, 
dermal contact or eyes 
contact. Where those 
risks cannot be 
eliminated by such 
procedures and 
measures, the additive 
and premixtures shall 
be used with personal 
skin, eye and breathing 
protective equipment . All Suidae  
for fattening  76 
Sows of all 
Suidae  
species  94 
Calves 
(milk 
replacers)   
 
Sheep  and 
goats  
 
Other 
ruminants  
for fattening  
 95 
Horses  141 
Dairy 
ruminants  
other than  
sheep and 
goats  91 
Salmonids , 
ornamental 
fish 
and all other 
fin fish  
 
Dogs  60 
                                                           
(19) Details of the analytical methods are available at the following address of the Reference Laboratory: https://ec.europa.eu/jrc/en/eurl/feed -additives/evaluation -reports  19 
 Other 
animal 
species  and 
categories  30 
 
2b141 -eo - Distilled 
lime 
essential oil   
 Additive composition  
Distilled l ime essential oil  obtained by 
unpeeled fruits of the  plant species 
Citrus aurantiifolia  (Christm.) Swingle  
Liquid form  
 
Characterisation of the active 
substance  
Distilled l ime essential oil  obtained by 
steam distillation from unpeeled fruits 
of the  plant species Citrus aurantiifolia 
(Christm.) Swingle  as defined by the 
Council of Europe (20) 
 
The specifications of the active 
substance are : 
d-Limonene: 45 –52 %  
γ-Terpinene: 10 –14 %  
Terpinolene: 5.5 –10.5%  
αTerpineo l: 6–8%  
β-Caryophyllene: 0.2 –0.8%  
Furocoumarins: ≤ 0.0083 %  
Methoxycoumarins: ≤ 0.03%  
 Chickens 
for fattening  
and minor 
poultry 
species for 
fattening  - - 8.5 1. The additive shall be 
incorporated into the 
feed in the form of a 
premixture.  
2. In the directions for use 
of the additive and 
premixtures, the storage 
conditions and stability 
to heat treatment shall 
be indicated.  
3. Mixture of distilled l ime 
essential oil  with other 
botanical additives is 
permitted provided that 
the amounts of 
furocouma rins, and 
methoxycoumarins in 
feed materials and 
compound feed are 
lower than the one 
resulting from the use of 
a single additive at the 
maximum or 
recommended level for 
the species or animal 
category.  29 
September 
2032  
Laying hens  
and minor 
poultry  
species for 
laying or 
breeding  12.5 
Turkeys for 
fatening  11 
Piglets  of all 
Suidae  
species  15 
All Suidae  
for fattening  18 
Lactating 
sows  of all 
Suidae  
species  22 
                                                           
(20) Natural sources of flavourings - Report No. 2 (2007).  20 
 CoE number :141 
 
Analytical method (21) 
For the quantification of phytochemical 
marker d-limonene  in the feed additive:  
- Gas chromatography coupled with 
flame ionisation detection (GC -
FID) (based on ISO 3519 ) 
 
 Sheep  and 
goats  
 
Other 
ruminants  
for fattening  
 
Horses  33.5 4. For users of the additive 
and premixtures, feed 
busine ss operators shall 
establish operational 
procedures and 
organisational measures 
to address potential 
risks by inhalation, 
dermal contact or eyes 
contact. Where those 
risks cannot be 
eliminated by such 
procedures and 
measures, the additive 
and premixtures s hall be 
used with personal skin, 
eye and breathing 
protective equipment.  Calves 
(milk 
replacers)  35.5 
Dairy 
ruminants 
other than 
sheep and 
goats  21.5 
 
Rabbits  13.5 
Salmonids  
and other fin 
fish except 
ornemental 
fish 30 
Other 
animal 
species and 
categories 
except dogs, 
cats, 
ornamental 
fish and 
ornamental 
birds  8.5 
  
                                                           
(21) Details of the analytical methods are availabl e at the following address of the Reference Laboratory: https://ec.europa.eu/jrc/en/eurl/feed -additives/evaluation -reports  21 
 ANNEX  IV 
 
Identifi -
cation 
number 
of the 
additive  Name of 
the holder 
of 
authorisa -
tion Additive  Composition, chemical formula, 
description, analytical method.  Species or 
category of 
animal  Maxi -
mum 
age Minimum 
content  Maximum 
content  
Other provisions  End of 
period of 
authori -
sation  mg active substance /kg 
of complete feed with a 
moisture content of 
12%  
Category: Sensory additives. Functional group: Flavouring compounds  
2b142 -
eo - Expressed 
mandarin 
essential 
oil  Additive composition  
Mandarin essential oil 
obtained from fruit peel of 
Citrus reticulata  Blanco . 
Liquid form  
 
Characterisation of the active 
substance  
Expressed  mandarin essential 
oil obtained by cold 
expression from fruit peel of 
Citrus reticulata  Blanco as 
defined by the Council of 
Europe  (22). 
 
d-Limonene: 65 –80% 
-Terpinene: 13 -22% Poultry  
Rabbits  
Salmonids  - - 15 1. The additive shall be 
incorporated into the feed in the 
form of a premixture.  
2. In the directions for use of the 
additive and premixtures, the 
storage conditions and stability 
to heat treatment shall be 
indicated.  
3. Mixture of expressed mandarin 
essential oil with other botanical 
additives is permitted provided 
that the amounts of perillaldehy de 
in feed materials and compound 
feed are lower than the one 
resulting from the use of a single 
additive at the maximum or 
recommended level for the 
specie s or animal category.  
4. For users of the additive and 
premixtures, feed business 20 March 
2032  
 
Suidae   - - 33 
Ruminants  - - 30 
                                                           
(22) Natural sources of flavourings - Report No. 2 (2007)  22 
 -Pinene  (pin-2(3)-ene):  
1-3.5 %  
Myrcene:1 -2% 
β-Pinene (pin -2(10) -ene):  
1-2%. 
Methyl N -methyl anthranilate: 
0.15-0.7%  
Perillaldehyde: ≤ 0.063%  
 
CAS number:  8008 -31-9  
FEMA number: 2657  
CoE number: 142  
 
Analytical method  (23) 
For the quantification of 
phytochemical  marker  d-
limonene  in the feed additive : 
 Gas chromatography 
coupled with flame 
ionisation detection (GC -
FID) (based on ISO 3528)  Horses  - - 40 operators shall estab lish 
operational procedures and 
organisational measures to 
address potential risks by 
inhalation, dermal contact or eyes 
contact. Where those risks cannot 
be eliminated by such procedures 
and measures, the additive and 
premixtures shall be used with 
person al skin, eye and breathing 
protective equipment.  
Other animal 
species and 
categories  
except dogs, 
cats, 
ornamental 
fish and 
ornamental 
birds  15 
 
 
                                                           
(23) Details of the analytical methods are available at the following address of the Reference Laboratory: https://ec.europa.eu/jrc/en/eurl/feed -additives/evaluation -reports