COMMISSION IMPLEMENTING REGUL ATION (EU) 2021/2093 
of 29 November 2021
concer ning the author isation of disodium 5'-guany late as a feed additiv e for all animal species 
(Text with EEA relevance) 
THE EUR OPEAN COMMISSION,
Havi ng regar d to the Treaty on the Functioning of the European Union,
Havi ng rega rd to Regulation (EC) No 1831/2003 of the European Parliament and of the Council of 22 Sept ember 2003 on 
additives for use in animal nutr ition (1), and in particular Article 9(2) thereof,
Whereas:
(1) Regulation (EC) No 1831/2003 provid es for the author isation of additives for use in animal nutr ition and for the 
grounds and procedures for granting such author isation.
(2) In accordance with Article 7 of Regulation (EC) No 1831/2003 an application was submitted for the author isation 
of disodium 5'-guanyla te. That application was accompan ied by the particulars and documents required under 
Article 7(3) of Regulation (EC) No 1831/2003.
(3) The application concer ns the author isation of disodium 5'-guanyl ate as a feed additive for all animal species, to be 
classifie d in the categor y ‘sensor y additives ’ and in the functional group ‘flavouring compounds’.
(4) The applicant requeste d disodium 5'-guan ylate to be author ised also for use in water for drinking. Howe ver, 
Regulation (EC) No 1831/2003 does not allow the author isation of ‘flavouring compo unds ’ for use in water for 
drinking. Theref ore, the use of disodium 5'-guanyl ate in water for drinking should not be allowed.
(5) The European Food Safety Author ity (‘the Author ity’) concluded in its opinion of 5 May 2021 (2)that, under the 
proposed conditions of use, disodium 5'-guanyl ate does not have adverse effects on animal health, human health or 
the environment.
(6) The Author ity further concluded, that disodium 5'-guanylat e is efficacious to contr ibut e to the flavour of feed. The 
Authority also verified the repor t on the methods of analysis of the feed additive in feed submitted by the Reference 
Laboratory set up by Regulation (EC) No 1831/2003.
(7) The assessment of disodium 5'-guanylat e shows that the conditions for author isation, as provi ded for in Article 5 of 
Regulation (EC) No 1831/2003, are satisfi ed. According ly, the use of that substance should be author ised as specifi ed 
in the Annex to this Regulation.
(8) Certain conditions should be provid ed for to allow better control. In particular , a recommended content should be 
indicated on the label of the feed additives. Where such content is exceeded, certain information should be indicated 
on the label of premixtures.
(9) The fact that disodium 5'-guan ylate is not author ised for use as a flavouri ng in water for drinking, does not preclude 
its use in compo und feed whic h is administ ered via water .
(10) The measures provid ed for in this Regulation are in accordance with the opinion of the Standing Committ ee on 
Plants, Animals, Food and Feed,
(1)OJ L 268, 18.10.2003, p. 29.
(2)EFSA Jour nal 2021;19(6):6619.EN Offi cial Jour nal of the European Union 30.11.2021 L 427/169  HAS ADOPTED THIS REGUL ATION:
Article 1
The substance specified in the Annex, belonging to the additive cate gory ‘sensor y additives’ and to the functional group 
‘flavouring compounds’, is author ised as an additive in animal nutr ition, subject to the conditions laid down in that Annex.
Article 2
This Regulation shall enter into force on the twentieth day followi ng that of its publication in the Official Journal of the 
European Union.
This Regulation shall be binding in its entirety and directly applicable in all Member States.
Done at Brussels, 29 November 2021 .
For the Commission
The President
Ursula VON DER LEYENEN Offi cial Jour nal of the European Union L 427/170 30.11.2021  ANNEX 
Identification 
number of the 
additiveName of the 
holder of 
author isationAdditiveComposition, chemical formula, descr iption, 
analytical methodSpecies or 
categor y of 
animalMaximum 
ageMinimum 
conten tMaximum 
conten t
Other provisionsEnd of period of 
author isationmg active substance/kg 
of complet e feed with a 
moisture conten t of 
12 %
Categor y: Sensor y additiv es
Functional group: Flavour ing compounds
2b627i - Disodium 5'- 
guany lateAdditive composition
Disodium 5'-guanylat e (GMP).
Powder form
Char acter isation of the active substance
Disodium 5'-guanylat e (hydrat ed form) 
produced with Corynebacter ium stationis 
KCCM 10530 and Escher ichia coli K-12 
KFCC 11067.
Produced by fermentation
Purity: min.: 97 %
Chemical formula:
C10H12N5Na 2O8P
CAS number: 5550-12-9
Einecs number: 226-914-1
Analytical method (1)
For the identification of disodium 5'- 
guany late (GMP) in the feed additive:
— FAO JECF A monograph ‘disodium 
5'-guanyl ate’All 
animal 
species- - - 1.The additive shall be incor porat ed 
into the feed in the form of a pre­
mixture.
2.In the directions for use of the ad­
ditive and premixtures, the storage 
conditions and stability to heat 
treatment shall be indicated.
3.On the label of the additive the fol­
lowing shall be indicate d:
‘Recommended maximum conte nt 
of the active substance when used 
alone or in combination with other 
ribonucleotides up to the same 
level per kg of compl ete 
feedingstuff with a moisture 
cont ent of 12 %: 50 mg’.
4.The functional group, the identif i­
cation number , the name and the 
added amount of the active sub­
stance shall be indicate d on the label 
of the premixture where the use le­
vel on the label of the premixture 
would result in exceeding the level 
of active substance in complet e fee­
dingstuff refer red to in point 3.20 December 
2031EN Offi cial Jour nal of the European Union 30.11.2021 L 427/171  For the determination of disodium 5'- 
guany late (GMP) in the feed additive, 
flavouring premixtures and water:
—High performa nce liquid chromat o­
graphy coupled to UV detection 
(HPL C-UV)
(1)Details of the analytical methods are avail able at the follo wing address of the Reference Laboratory : https://ec.europa.eu/jrc/en/eurl/feed-additives/evaluation-repor tsEN Offi cial Jour nal of the European Union L 427/172 30.11.2021