COMMISSION IMPLEMENTING REGUL ATION (EU) 2022/1250 
of 19 July 2022
concer ning the author isation of ethy l acrylate, pentyl isovalerate, butyl 2-methyl butyrate, 
2-methy lundecanal, (2E)-methylcrot onic acid, ethy l (E,Z)-deca-2,4-dienoate, butan -2-one, cyclohexyl 
acet ate, 3,4-dimeth ylcy clopent an-1,2-dione, 5-ethy l-3-hydro xy-4-methylfuran-2(5H)-one, phenethyl 
butyrate, hexyl pheny lacet ate, 4-methylacet ophenone, 4-metho xyacet ophenone, 3-methylphenol, 
3,4-dimethylpheno l, 1-metho xy-4-methy lbenzene, trimethylo xazole and 4,5-dih ydrothiophen-3 
(2H)-one as feed additiv es for all animal species 
(Text with EEA relevance) 
THE EUR OPEAN COMMISSION,
Havi ng regar d to the Treaty on the Functioning of the European Union,
Havi ng rega rd to Regulation (EC) No 1831/2003 of the European Parliament and of the Council of 22 Sept ember 2003 on 
additives for use in animal nutr ition (1), and in particular Article 9(2) thereof,
Whereas:
(1) Regulation (EC) No 1831/2003 provid es for the author isation of additives for use in animal nutr ition and for the 
grounds and procedures for granting such an author isation. Article 10(2) of that Regulation provides for the 
re-evaluation of additives author ised pursuant to Council Directive 70/524/EEC (2).
(2) The substances ethyl acrylate, pentyl isovalerate, butyl 2-methyl butyrat e, 2-methylundecanal, (2E)-methylcrot onic 
acid, ethyl (E,Z)-deca-2,4-dienoate , butan-2-one, cyclohexyl acetate , 3,4-dimethylcy clopentan-1,2-dione, 5-ethy l- 
3-hydro xy-4-meth ylfuran-2(5H)-one, phenethyl butyrate, hexyl pheny lacetate, 4-meth ylacetophenone, 
4-metho xyacet ophenone, 3-methyl phenol, 3,4-dimethyl phenol, 1-metho xy-4-meth ylbenzene, trimethyl oxazole 
and 4,5-dihydrothi ophen-3(2H)-one were author ised without a time limit in accordance with Directive 70/524/EEC 
as a feed additives for all animal species. Those substances were subsequently entered in the Regist er of feed additives 
as existing products, in accordance with Article 10(1)(b) of Regulation (EC) No 1831/2003.
(3) In accordance with Article 10(2) of Regulation (EC) No 1831/2003 in conjunction with Article 7 thereof, several 
applications were submitted for the re-evaluation of those additives for all animal species.
(4) The applicant requested the additives to be author ised for use also in water for drinking. However , Regulation (EC) 
No 1831/2003 does not allow the author isation of flavouring compo unds for use in water for drinking. Theref ore, 
the use of these additives in wate r for drinking should not be allowed.
(5) The applicant requeste d the additives to be classified in the additive cate gory ‘sensor y additives ’ and in the functional 
group ‘flavouring compo unds ’. Those applications were accompanied by the particulars and documents required 
under Article 7(3) of Regulation (EC) No 1831/2003.
(1)OJ L 268, 18.10.2003, p. 29.
(2)Council Directive 70/524/EEC of 23 November 1970 concer ning additives in feedingstuffs (OJ L 270, 14.12.1970, p. 1).EN Offi cial Jour nal of the European Union 20.7.2022 L 191/13  (6) The European Food Safet y Author ity (‘the Author ity’) concluded in its opinions of 1 Febr uary 2012 (3), 7 March 
2012 (4), 25 Apr il 2012 (5), 17 October 2012 (6), 12 Marc h 2013 (7), 5 March 2014 (8), 20 October 2015 (9), 
26 Januar y 2016 (10), 8 Marc h 2016 (11), 20 Apr il 2016 (12), 25 May 2016 (13), 12 July 2016 (14)and 19 October 
2016 (15), that, under the proposed conditions of use, the additives do not have adverse effects on animal health, 
consumer health or the environment. The Auth ority also concluded that the additives should be considered as 
irritants to skin, eyes and respirato ry tract and as skin sensitizers. Theref ore, the Commission considers that 
appropr iate prote ctive measures should be taken to prevent adverse effects on human health, in particular as 
regards the users of the additive.
(7) The Authority concluded, that the additives are recognised to flavour food and its function in feed would be 
essentially the same as that in food, theref ore, no further demonstration of efficacy is considered necessar y. It also 
verified the repor t on the methods of analysis of the feed additives in feed submitte d by the Reference Laboratory 
set up by Regulation (EC) No 1831/2003.
(8) The assessment of the additives shows that the conditions for author isation, as provided for in Article 5 of 
Regulation (EC) No 1831/2003, are satisfied. According ly, the use of those substances should be author ised as 
specifi ed in the Annex to this Regulation.
(9) Certain conditions should be provid ed for to allow better control. In particular , a recommended content should be 
indicated on the label of the feed additives. Where such content is exceeded, certain information should be indicated 
on the label of premixtures.
(10) The fact that the additives are not author ised for use as a flavourings in water for drinking does not preclude their 
use in compo und feed which is administe red via water .
(11) Since safet y reasons do not require the immediate application of the modifications to the conditions of author isation 
of the substances concer ned, it is appropr iate to allow a transitional period for interest ed parties to prepare 
themselves to meet the new requirements resulting from the author isation.
(12) The measures provid ed for in this Regulation are in accordance with the opinion of the Standing Committ ee on 
Plants, Animals, Food and Feed,
HAS ADOPTED THIS REGUL ATION:
Article 1
Author isation
The substances specifi ed in the Annex, belonging to the additive cate gory ‘sensor y additives ’ and to the functional group 
‘flavouring compounds’, are author ised as additives in animal nutr ition, subject to the conditions laid down in that Annex.
(3)EFSA Journal 2012;10(2):2573.
(4)EFSA Journal 2012;10(3):2625.
(5)EFSA Journal 2012;10(5):2678.
(6)EFSA Journal 2012;10(10):2927.
(7)EFSA Journal 2013;11(4):3169.
(8)EFSA Journal 2014;12(3):3608.
(9)EFSA Journal 2015;13(11):4268.
(10)EFSA Journal 2016;14(2):4390.
(11)EFSA Journal 2016;14(6):4441.
(12)EFSA Journal 2016;14(6):4475.
(13)EFSA Journal 2016;14(6):4512.
(14)EFSA Journal 2016;14(8):4557.
(15)EFSA Journal 2016;14(11):4618.EN Offi cial Jour nal of the European Union L 191/14 20.7.2022  Article 2
Transitional measures
1. The substances specifi ed in the Annex and premixtures containing these substances, which are produced and labelled 
before 9 Febr uary 2023 in accordance with the rules applicable before 9 August 2022 may continue to be placed on the 
mark et and used until the existing stoc ks are exhaust ed.
2. Com pound feed and feed mate rials containing these substances as specified in the Annex which are produced and 
labelled before 9 August 2023 in accordance with the rules applicable before 9 August 2022 may continue to be placed on 
the mark et and used until the existing stoc ks are exhaust ed if they are intended for food-producing animals.
3. Com pound feed and feed mate rials containing these substances as specified in the Annex which are produced and 
labelled before 9 August 2024 in accordance with the rules applicable before 9 August 2022 may continue to be placed on 
the mark et and used until the existing stoc ks are exhaust ed if they are intended for non-f ood-producing animals.
Article 3
Entr y into force
This Regulation shall enter into force on the twentieth day followi ng that of its publication in the Official Journal of the 
European Union.
This Regulation shall be binding in its entirety and directly applicable in all Member States.
Done at Brussels, 19 July 2022 .
For the Commission
The President
Ursula VON DER LEYENEN Offi cial Jour nal of the European Union 20.7.2022 L 191/15  ANNEX 
Identification 
number of 
the additiveAdditiveCompo sition, chemical 
formula, descr iption, 
analytical methodSpecies or 
categor y 
of animalMaxi -
mum 
ageMinimum 
conten tMaximum 
conten t
Other provisi onsEnd of period of 
author isationmg of additive/kg of 
complet e feedingstuff 
with a moisture content of 
12 %
Categor y: Sensor y additiv es
Functional group: Flavour ing compounds
2b09037 Ethyl 
acrylateAdditive composition
Ethyl acrylate
Char acter isation of active 
substance
Ethyl acrylate
Produced by chemical 
synthesis
Purity: 97 %
Chemical formula: 
C5H8O2
CAS number: 140-88-5
FLAVIS: 09.037All 
animal 
species- - - 1.The additive shall be incor porate d into the feed in the form of a 
premixture.
2.In the directions for use of the additive and premixtures, the storage 
conditions and stability to heat treatment shall be indicate d.
3.On the label of the additive the following shall be indicated :
‘Recommended maximum content of the active substance per kg of 
compl ete feedingstuff with a moisture cont ent of 12 %:
— pigs and poultr y: 1 mg;
— other species or categor ies of animals: 1,5 mg.’
4.The functional group, the identif ication number , the name and the 
added amount of the active substance shall be indicated on the label 
of the premixture where the use level on the label of the premixture 
would result in exceed ing the level refer red to in point 3.
5.For users of the additive and premixtures, feed business operat ors 
shall establish operational procedures and organisational measures 
to address poten tial risks by inhalation, dermal contact or eyes 
contact. Where those risks cannot be eliminated or reduced to a 
minimum by such procedures and measures, the additive and 
premixtures shall be used with personal protective equipment, 
including skin, eye and breathing prote ction.9 August 2032
Analytical method (1)
For the identification of 
ethyl acrylate in the feed 
additive and in feed 
flavouring premixtures:
Gas chromat ography 
mass spectrometr y with 
retention time locking 
GC-MS-RTL.
(1)Details of the analytical methods are available at the following address of the Reference Laborato ry: https://joint-research-c entre.ec.europa.eu/eurl-fa-eurl-f eed-additives/eurl-fa-author isation/eurl-f a-evaluation- 
repor ts_enEN Offi cial Jour nal of the European Union L 191/16 20.7.2022  Identification 
number of 
the additiveAdditiveCompo sition, chemical 
formula, descr iption, 
analytical methodSpecies or 
categor y 
of animalMaxi -
mum 
ageMinimum 
conten tMaximum 
conten t
Other provisi onsEnd of period of 
author isationmg of additive/kg of 
complet e feedingstuff 
with a moisture content of 
12 %
Categor y: Sensor y additiv es
Functional group: Flavour ing compounds
2b09499 Pentyl 
isovaler ateAdditive composition
Pentyl isovalerat e
Char acter isation of active 
substance
Pentyl isovalerat e
Produced by chemical 
synthesis
Purity: 98 %
Chemical formula: 
C10H20O2
CAS number: 
25415-62-7
FLAVIS: 09.499All 
animal 
species- - - 1.The additive shall be incor porate d into the feed in the form of a 
premixture.
2.In the directions for use of the additive and premixtures, the storage 
conditions and stability to heat treatment shall be indicate d.
3.On the label of the additive the following shall be indicated :
‘Recommended maximum content of the active substance per kg of 
compl ete feedingstuff with a moisture cont ent of 12 %:
— pigs and poultr y: 1 mg;
— other species or categor ies of animals: 1,5 mg.’
4.The functional group, the identif ication number , the name and the 
added amount of the active substance shall be indicated on the label 
of the premixture where the use level on the label of the premixture 
would result in exceed ing the level refer red to in point 3.
5.For users of the additive and premixtures, feed business operat ors 
shall establish operational procedures and organisational measures 
to address poten tial risks by inhalation, dermal contact or eyes 
contact. Where those risks cannot be eliminated or reduced to a 
minimum by such procedures and measures, the additive and 
premixtures shall be used with personal protective equipment, 
including skin, eye and breathing prote ction.9 August 2032
Analytical method (1)
For the identification of 
pentyl isovalera te in the 
feed additive and in feed 
flavouring premixtures:
Gas chromat ography 
mass spectrometr y with 
retention time locking 
GC-MS-RTL.
(1)Details of the analytical methods are available at the following address of the Reference Laborato ry: https://joint-research-c entre.ec.europa.eu/eurl-fa-eurl-f eed-additives/eurl-fa-author isation/eurl-f a-evaluation- 
repor ts_enEN Offi cial Jour nal of the European Union 20.7.2022 L 191/17  Identification 
number of 
the additiveAdditiveCompo sition, chemical 
formula, descr iption, 
analytical methodSpecies or 
categor y 
of animalMaxi -
mum 
ageMinimum 
conten tMaximum 
conten t
Other provisi onsEnd of period of 
author isationmg of additive/kg of 
complet e feedingstuff 
with a moisture content of 
12 %
Categor y: Sensor y additiv es
Functional group: Flavour ing compounds
2b09519 Butyl 
2-methyl 
butyrateAdditive composition
Butyl 2-methyl butyrat e
Char acter isation of active 
substance
Butyl 2-methyl butyrat e
Produced by chemical 
synthesis
Purity: 95 %
Chemical formula: 
C9H18O2
CAS number: 
15706-73-7
FLAVIS: 09.519All 
animal 
species- - - 1.The additive shall be incor porate d into the feed in the form of a 
premixture.
2.In the directions for use of the additive and premixtures, the storage 
conditions and stability to heat treatment shall be indicate d.
3.On the label of the additive the following shall be indicated :
‘Recommended maximum content of the active substance per kg of 
compl ete feedingstuff with a moisture cont ent of 12 %:
— pigs and poultr y: 1 mg;
— other species or categor ies of animals: 1,5 mg.’
4.The functional group, the identif ication number , the name and the 
added amount of the active substance shall be indicated on the label 
of the premixture where the use level on the label of the premixture 
would result in exceed ing the level refer red to in point 3.
5.For users of the additive and premixtures, feed business operat ors 
shall establish operational procedures and organisational measures 
to address poten tial risks by inhalation, dermal contact or eyes 
contact. Where those risks cannot be eliminated or reduced to a 
minimum by such procedures and measures, the additive and 
premixtures shall be used with personal protective equipment, 
including skin, eye and breathing prote ction.9 August 2032
Analytical method (1)
For the identification of 
butyl 2-methyl butyrat e 
in the feed additive and in 
feed flavouri ng 
premixtures:
Gas chromat ography 
mass spectrometr y with 
retention time locking 
GC-MS-RTL.
(1)Details of the analytical methods are available at the following address of the Reference Laborato ry: https://joint-research-c entre.ec.europa.eu/eurl-fa-eurl-f eed-additives/eurl-fa-author isation/eurl-f a-evaluation- 
repor ts_enEN Offi cial Jour nal of the European Union L 191/18 20.7.2022  Identification 
number of 
the additiveAdditiveCompo sition, chemical 
formula, descr iption, 
analytical methodSpecies or 
categor y 
of animalMaxi -
mum 
ageMinimum 
conten tMaximum 
conten t
Other provisi onsEnd of period of 
author isationmg of additive/kg of 
complet e feedingstuff 
with a moisture content of 
12 %
Categor y: Sensor y additiv es
Functional group: Flavour ing compounds
2b05077 2-Methy lun-
decanalAdditive composition
2-Methy lundecanal
Char acter isation of active 
substance
2-Methy lundecanal
Produced by chemical 
synthesis
Purity: 97 %
Chemical formula: 
C12H24O
CAS number: 110-41-8
FLAVIS: 05.077All 
animal 
species- - - 1.The additive shall be incor porate d into the feed in the form of a 
premixture.
2.In the directions for use of the additive and premixtures, the storage 
conditions and stability to heat treatment shall be indicate d.
3.On the label of the additive the following shall be indicated :
‘Recommended maximum content of the active substance per kg of 
compl ete feedingstuff with a moisture cont ent of 12 %:
— pigs and poultr y: 1 mg;
— other species or categor ies of animals: 1,5 mg.’
4.The functional group, the identif ication number , the name and the 
added amount of the active substance shall be indicated on the label 
of the premixture where the use level on the label of the premixture 
would result in exceed ing the level refer red to in point 3.
5.For users of the additive and premixtures, feed business operat ors 
shall establish operational procedures and organisational measures 
to address poten tial risks by inhalation, dermal contact or eyes 
contact. Where those risks cannot be eliminated or reduced to a 
minimum by such procedures and measures, the additive and 
premixtures shall be used with personal protective equipment, 
including skin, eye and breathing prote ction.9 August 2032
Analytical method (1)
For the identification of 
2-methyl undecanal in the 
feed additive and in feed 
flavouring premixtures:
Gas chromat ography 
mass spectrometr y with 
retention time locking 
GC-MS-RTL.- - -
(1)Details of the analytical methods are available at the following address of the Reference Laborato ry: https://joint-research-c entre.ec.europa.eu/eurl-fa-eurl-f eed-additives/eurl-fa-author isation/eurl-f a-evaluation- 
repor ts_enEN Offi cial Jour nal of the European Union 20.7.2022 L 191/19  Identification 
number of 
the additiveAdditiveCompo sition, chemical 
formula, descr iption, 
analytical methodSpecies or 
categor y 
of animalMaxi -
mum 
ageMinimum 
conten tMaximum 
conten t
Other provisi onsEnd of period of 
author isationmg of additive/kg of 
complet e feedingstuff 
with a moisture content of 
12 %
Categor y: Sensor y additiv es
Functional group: Flavour ing compounds
2b08064 (2E)- 
Methylcro -
tonic acidAdditive composition
(2E)-Methy lcrotonic acid
Char acter isation of active 
substance
(2E)-Methy lcrotonic acid
Produced by chemical 
synthesis
Purity: min. 99 %
Chemical formula: 
C5H8O2
CAS number: 80-59-1
FLAVIS: 08.064All 
animal 
species- - - 1.The additive shall be incor porate d into the feed in the form of a 
premixture.
2.In the directions for use of the additive and premixtures, the storage 
conditions and stability to heat treatment shall be indicate d.
3.On the label of the additive the following shall be indicated :
‘Recommended maximum content of the active substance per kg of 
compl ete feedingstuff with a moisture cont ent of 12 %:
— mar ine animals: 0,05 mg;
— other species or categor ies of animals: 1 mg.’
4.The functional group, the identif ication number , the name and the 
added amount of the active substance shall be indicated on the label 
of the premixture where the use level on the label of the premixture 
would result in exceed ing the level refer red to in point 3.
5.For users of the additive and premixtures, feed business operat ors 
shall establish operational procedures and organisational measures 
to address poten tial risks by inhalation, dermal contact or eyes 
contact. Where those risks cannot be eliminated or reduced to a 
minimum by such procedures and measures, the additive and 
premixtures shall be used with personal protective equipment, 
including skin, eye and breathing prote ction.9 August 2032
Analytical method (1)
For the identification of 
(2E)-methy lcrotonic acid 
in the feed additive and in 
feed flavouri ng 
premixtures:
Gas chromat ography 
mass spectrometr y with 
retention time locking 
GC-MS-RTL.
(1)Details of the analytical methods are available at the following address of the Reference Laborato ry: https://joint-research-c entre.ec.europa.eu/eurl-fa-eurl-f eed-additives/eurl-fa-author isation/eurl-f a-evaluation- 
repor ts_enEN Offi cial Jour nal of the European Union L 191/20 20.7.2022  Identification 
number of 
the additiveAdditiveCompo sition, chemical 
formula, descr iption, 
analytical methodSpecies or 
categor y 
of animalMaxi -
mum 
ageMinimum 
conten tMaximum 
conten t
Other provisi onsEnd of period of 
author isationmg of additive/kg of 
complet e feedingstuff 
with a moisture content of 
12 %
Categor y: Sensor y additiv es
Functional group: Flavour ing compounds
2b09260 Ethyl (E,Z)- 
dec-
a-2,4-dieno -
ateAdditive composition
Ethyl (E,Z)- 
deca-2,4-dienoat e
Char acter isation of active 
substance
Ethyl (E,Z)- 
deca-2,4-dienoat e
Produced by chemical 
synthesis
Purity: min. 90 %
Chemical formula: 
C12H20O2
CAS number: 3025-30-7
FLAVIS: 09.260All 
animal 
species- - - 1.The additive shall be incor porate d into the feed in the form of a 
premixture.
2.In the directions for use of the additive and premixtures, the storage 
conditions and stability to heat treatment shall be indicate d.
3.On the label of the additive the following shall be indicated :
‘Recommended maximum content of the active substance per kg of 
compl ete feedingstuff with a moisture cont ent of 12 %:
— mar ine animals: 0,05 mg;
— other species or categor ies of animals: 1 mg.’
4.The functional group, the identif ication number , the name and the 
added amount of the active substance shall be indicated on the label 
of the premixture where the use level on the label of the premixture 
would result in exceed ing the level refer red to in point 3.
5.For users of the additive and premixtures, feed business operat ors 
shall establish operational procedures and organisational measures 
to address poten tial risks by inhalation, dermal contact or eyes 
contact. Where those risks cannot be eliminated or reduced to a 
minimum by such procedures and measures, the additive and 
premixtures shall be used with personal protective equipment, 
including skin, eye and breathing prote ction.9 August 2032
Analytical method (1)
For the identification of 
ethyl (EZ)- 
deca-2,4-dienoat e in the 
feed additive and in feed 
flavouring premixtures:
Gas chromat ography 
mass spectrometr y with 
retention time locking 
GC-MS-RTL.
(1)Details of the analytical methods are available at the following address of the Reference Laborato ry: https://joint-research-c entre.ec.europa.eu/eurl-fa-eurl-f eed-additives/eurl-fa-author isation/eurl-f a-evaluation- 
repor ts_enEN Offi cial Jour nal of the European Union 20.7.2022 L 191/21  Identification 
number of 
the additiveAdditiveCompo sition, chemical 
formula, descr iption, 
analytical methodSpecies or 
categor y 
of animalMaxi -
mum 
ageMinimum 
conten tMaximum 
conten t
Other provisi onsEnd of period of 
author isationmg of additive/kg of 
complet e feedingstuff 
with a moisture content of 
12 %
Categor y: Sensor y additiv es
Functional group: Flavour ing compounds
2b07053 Butan- 
2-oneAdditive composition
Butan-2-one
Char acter isation of active 
substance
Butan-2-one
Produced by chemical 
synthesis
Purity: min. 99,5 %
Chemical formula: 
C4H8O
CAS number: 78-93-3
FLAVIS: 07.053All 
animal 
species- - - 1.The additive shall be incor porate d into the feed in the form of a 
premixture.
2.In the directions for use of the additive and premixtures, the storage 
conditions and stability to heat treatment shall be indicate d.
3.On the label of the additive the following shall be indicated :
‘Recommended maximum content of the active substance per kg of 
compl ete feedingstuff with a moisture cont ent of 12 %:
— pigs and poultr y: 1 mg;
— other species or categor ies of animals: 1,5 mg.’
4.The functional group, the identif ication number , the name and the 
added amount of the active substance shall be indicated on the label 
of the premixture where the use level on the label of the premixture 
would result in exceed ing the level refer red to in point 3.
5.For users of the additive and premixtures, feed business operat ors 
shall establish operational procedures and organisational measures 
to address poten tial risks by inhalation, dermal contact or eyes 
contact. Where those risks cannot be eliminated or reduced to a 
minimum by such procedures and measures, the additive and 
premixtures shall be used with personal protective equipment, 
including skin, eye and breathing prote ction.9 August 2032
Analytical method (1)
For the identification of 
butan-2-one in the feed 
additive and in feed 
flavouring premixtures:
Gas chromat ography 
mass spectrometr y with 
retention time locking 
GC-MS-RTL.
(1)Details of the analytical methods are available at the following address of the Reference Laborato ry: https://joint-research-c entre.ec.europa.eu/eurl-fa-eurl-f eed-additives/eurl-fa-author isation/eurl-f a-evaluation- 
repor ts_enEN Offi cial Jour nal of the European Union L 191/22 20.7.2022  Identification 
number of 
the additiveAdditiveCompo sition, chemical 
formula, descr iption, 
analytical methodSpecies or 
categor y 
of animalMaxi -
mum 
ageMinimum 
conten tMaximum 
conten t
Other provisi onsEnd of period of 
author isationmg of additive/kg of 
complet e feedingstuff 
with a moisture content of 
12 %
Categor y: Sensor y additiv es
Functional group: Flavour ing compounds
2b09027 Cyclohexyl 
acetateAdditive composition
Cyclohexyl acetate
Char acter isation of active 
substance
Cyclohexyl acetate
Produced by chemical 
synthesis
Purity: > 98 %
Chemical formula: 
C8H14O2
CAS number: 622-45-7
FLAVIS: 09.027All 
animal 
species- - - 1.The additive shall be incor porate d into the feed in the form of a 
premixture.
2.In the directions for use of the additive and premixtures, the storage 
conditions and stability to heat treatment shall be indicate d.
3.On the label of the additive the following shall be indicated :
‘Recommended maximum content of the active substance per kg of 
compl ete feedingstuff with a moisture cont ent of 12 %:
— mar ine animals: 0,05 mg;
— other species or categor ies of animals: 1 mg.’
4.The functional group, the identif ication number , the name and the 
added amount of the active substance shall be indicated on the label 
of the premixture where the use level on the label of the premixture 
would result in exceed ing the level refer red to in point 3.
5.For users of the additive and premixtures, feed business operat ors 
shall establish operational procedures and organisational measures 
to address poten tial risks by inhalation, dermal contact or eyes 
contact. Where those risks cannot be eliminated or reduced to a 
minimum by such procedures and measures, the additive and 
premixtures shall be used with personal protective equipment, 
including skin, eye and breathing prote ction.9 August 2032
Analytical method (1)
For the identification of 
cyclohexyl acetate in the 
feed additive and in feed 
flavouring premixtures:
Gas chromat ography 
mass spectrometr y with 
retention time locking 
GC-MS-RTL.
(1)Details of the analytical methods are available at the following address of the Reference Laborato ry: https://joint-research-c entre.ec.europa.eu/eurl-fa-eurl-f eed-additives/eurl-fa-author isation/eurl-f a-evaluation- 
repor ts_enEN Offi cial Jour nal of the European Union 20.7.2022 L 191/23  Identification 
number of 
the additiveAdditiveCompo sition, chemical 
formula, descr iption, 
analytical methodSpecies or 
categor y 
of animalMaxi -
mum 
ageMinimum 
conten tMaximum 
conten t
Other provisi onsEnd of period of 
author isationmg of additive/kg of 
complet e feedingstuff 
with a moisture content of 
12 %
Categor y: Sensor y additiv es
Functional group: Flavour ing compounds
2b07075 3,4-Dime -
thyl cyclo-
pen-
tan-1,2-dio -
neAdditive composition
3,4-Dimethylc yclopen -
tan-1,2-dione
Char acter isation of active 
substance
3,4-Dimethylc yclopen -
tan-1,2-dione
Produced by chemical 
synthesis
Purity: > 98 %
Chemical formula: 
C7H10O2
CAS number: 
13494-06-9
FLAVIS: 07.075All 
animal 
species- - - 1.The additive shall be incor porate d into the feed in the form of a 
premixture.
2.In the directions for use of the additive and premixtures, the storage 
conditions and stability to heat treatment shall be indicate d.
3.On the label of the additive the following shall be indicated :
‘Recommended maximum content of the active substance per kg of 
compl ete feedingstuff with a moisture cont ent of 12 %:
— cats and dogs: 5 mg;
— mar ine animals: 0,05 mg
— other species or categor ies of animals: 0,5 mg.’
4.The functional group, the identif ication number , the name and the 
added amount of the active substance shall be indicated on the label 
of the premixture where the use level on the label of the premixture 
would result in exceed ing the level refer red to in point 3.
5.For users of the additive and premixtures, feed business operat ors 
shall establish operational procedures and organisational measures 
to address poten tial risks by inhalation, dermal contact or eyes 
contact. Where those risks cannot be eliminated or reduced to a 
minimum by such procedures and measures, the additive and 
premixtures shall be used with personal protective equipment, 
including skin, eye and breathing prote ction.9 August 2032
Analytical method (1)
For the identification of 
3,4-dimethyl cyclopen -
tan-1,2-dione in the feed 
additive and in feed 
flavouring premixtures:
Gas chromat ography 
mass spectrometr y with 
retention time locking 
GC-MS-RTL.
(1)Details of the analytical methods are available at the following address of the Reference Laborato ry: https://joint-research-c entre.ec.europa.eu/eurl-fa-eurl-f eed-additives/eurl-fa-author isation/eurl-f a-evaluation- 
repor ts_enEN Offi cial Jour nal of the European Union L 191/24 20.7.2022  Identification 
number of 
the additiveAdditiveCompo sition, chemical 
formula, descr iption, 
analytical methodSpecies or 
categor y 
of animalMaxi -
mum 
ageMinimum 
conten tMaximum 
conten t
Other provisi onsEnd of period of 
author isationmg of additive/kg of 
complet e feedingstuff 
with a moisture content of 
12 %
Categor y: Sensor y additiv es
Functional group: Flavour ing compounds
2b10023 5-Ethyl- 
3-hydro xy- 
4-methyl -
furan-2 
(5H)-oneAdditive composition
5-Ethyl-3- hydro xy- 
4-methyl furan-2(5H)- 
one
Char acter isation of active 
substance
5-Ethyl-3- hydro xy- 
4-methyl furan-2(5H)- 
one
Produced by chemical 
synthesis
Purity: 95 %
Chemical formula: 
C7H10O3
CAS number: 698–10–2
FLAVIS: 10.023All 
animal 
species- - - 1.The additive shall be incor porate d into the feed in the form of a 
premixture.
2.In the directions for use of the additive and premixtures, the storage 
conditions and stability to heat treatment shall be indicate d.
3.On the label of the additive the following shall be indicated :
‘Recommended maximum content of the active substance per kg of 
compl ete feedingstuff with a moisture cont ent of 12 %:
— mar ine animals: 0,05 mg;
— poultr y and pigs: 0,05 mg;
— other species or categor ies of animals: 0,08 mg.’
4.The functional group, the identif ication number , the name and the 
added amount of the active substance shall be indicated on the label 
of the premixture where the use level on the label of the premixture 
would result in exceed ing the level refer red to in point 3.
5.For users of the additive and premixtures, feed business operat ors 
shall establish operational procedures and organisational measures 
to address poten tial risks by inhalation, dermal contact or eyes 
contact. Where those risks cannot be eliminated or reduced to a 
minimum by such procedures and measures, the additive and 
premixtures shall be used with personal protective equipment, 
including skin, eye and breathing prote ction.9 August 2032
Analytical method (1)
For the identification of 
5-ethy l-3-hydro xy- 
4-methyl furan-2(5H)- 
one in the feed additive 
and in feed flavouring 
premixtures:
Gas chromat ography 
mass spectrometr y with 
retention time locking 
GC-MS-RTL.
(1)Details of the analytical methods are available at the following address of the Reference Laborato ry: https://joint-research-c entre.ec.europa.eu/eurl-fa-eurl-f eed-additives/eurl-fa-author isation/eurl-f a-evaluation- 
repor ts_enEN Offi cial Jour nal of the European Union 20.7.2022 L 191/25  Identification 
number of 
the additiveAdditiveCompo sition, chemical 
formula, descr iption, 
analytical methodSpecies or 
categor y 
of animalMaxi -
mum 
ageMinimum 
conten tMaximum 
conten t
Other provisi onsEnd of period of 
author isationmg of additive/kg of 
complet e feedingstuff 
with a moisture content of 
12 %
Categor y: Sensor y additiv es
Functional group: Flavour ing compounds
2b09168 Phenethyl 
butyrateAdditive composition
Phenethyl butyrat e
Char acter isation of active 
substance
Phenethyl butyrat e
Produced by chemical 
synthesis
Purity: 97 %
Chemical formula: 
C12H16O2
CAS number: 103-52-6
FLAVIS: 09.168All 
animal 
species- - - 1.The additive shall be incor porate d into the feed in the form of a 
premixture.
2.In the directions for use of the additive and premixtures, the storage 
conditions and stability to heat treatment shall be indicate d.
3.On the label of the additive the following shall be indicated :
‘Recommended maximum content of the active substance per kg of 
compl ete feedingstuff with a moisture cont ent of 12 %:
— pigs and poultr y: 1 mg;
— other species or categor ies of animals: 1,5 mg.’
4.The functional group, the identif ication number , the name and the 
added amount of the active substance shall be indicated on the label 
of the premixture where the use level on the label of the premixture 
would result in exceed ing the level refer red to in point 3.
5.For users of the additive and premixtures, feed business operat ors 
shall establish operational procedures and organisational measures 
to address poten tial risks by inhalation, dermal contact or eyes 
contact. Where those risks cannot be eliminated or reduced to a 
minimum by such procedures and measures, the additive and 
premixtures shall be used with personal protective equipment, 
including skin, eye and breathing prote ction.9 August 2032
Analytical method (1)
For the identification of 
pheneth yl butyrat e in the 
feed additive and in feed 
flavouring premixtures:
Gas chromat ography 
mass spectrometr y with 
retention time locking 
GC-MS-RTL.- - -
(1)Details of the analytical methods are available at the following address of the Reference Laborato ry: https://joint-research-c entre.ec.europa.eu/eurl-fa-eurl-f eed-additives/eurl-fa-author isation/eurl-f a-evaluation- 
repor ts_enEN Offi cial Jour nal of the European Union L 191/26 20.7.2022  Identification 
number of 
the additiveAdditiveCompo sition, chemical 
formula, descr iption, 
analytical methodSpecies or 
categor y 
of animalMaxi -
mum 
ageMinimum 
conten tMaximum 
conten t
Other provisi onsEnd of period of 
author isationmg of additive/kg of 
complet e feedingstuff 
with a moisture content of 
12 %
Categor y: Sensor y additiv es
Functional group: Flavour ing compounds
2b09804 Hexyl 
pheny lace-
tateAdditive composition
Hexyl phenylacetat e
Char acter isation of active 
substance
Hexyl phenylacetat e
Produced by chemical 
synthesis
Purity: 97 %
Chemical formula: 
C14H20O2
CAS number: 5421-17-0
FLAVIS: 09.804All 
animal 
species- - - 1.The additive shall be incor porate d into the feed in the form of a 
premixture.
2.In the directions for use of the additive and premixtures, the storage 
conditions and stability to heat treatment shall be indicate d.
3.On the label of the additive the following shall be indicated :
‘Recommended maximum content of the active substance per kg of 
compl ete feedingstuff with a moisture cont ent of 12 %:
— pigs and poultr y: 1 mg;
— other species or categor ies of animals: 1,5 mg.’
4.The functional group, the identif ication number , the name and the 
added amount of the active substance shall be indicated on the label 
of the premixture where the use level on the label of the premixture 
would result in exceed ing the level refer red to in point 3.
5.For users of the additive and premixtures, feed business operat ors 
shall establish operational procedures and organisational measures 
to address poten tial risks by inhalation, dermal contact or eyes 
contact. Where those risks cannot be eliminated or reduced to a 
minimum by such procedures and measures, the additive and 
premixtures shall be used with personal protective equipment, 
including skin, eye and breathing prote ction.9 August 2032
Analytical method (1)
For the identification of 
hexyl pheny lacetate in the 
feed additive and in feed 
flavouring premixtures:
Gas chromat ography 
mass spectrometr y with 
retention time locking 
GC-MS-RTL.- - -
(1)Details of the analytical methods are available at the following address of the Reference Laborato ry: https://joint-research-c entre.ec.europa.eu/eurl-fa-eurl-f eed-additives/eurl-fa-author isation/eurl-f a-evaluation- 
repor ts_enEN Offi cial Jour nal of the European Union 20.7.2022 L 191/27  Identification 
number of 
the additiveAdditiveCompo sition, chemical 
formula, descr iption, 
analytical methodSpecies or 
categor y 
of animalMaxi -
mum 
ageMinimum 
conten tMaximum 
conten t
Other provisi onsEnd of period of 
author isationmg of additive/kg of 
complet e feedingstuff 
with a moisture content of 
12 %
Categor y: Sensor y additiv es
Functional group: Flavour ing compounds
2b07022 4-Methy la-
cetophe -
noneAdditive composition
4-Methy lacetophenone
Char acter isation of active 
substance
4-Methy lacetophenone
Produced by chemical 
synthesis
Purity: > 95 %
Chemical formula: 
C9H10O
CAS number: 122-00-9
FLAVIS: 07.022All 
animal 
species- - - 1.The additive shall be incor porate d into the feed in the form of a 
premixture.
2.In the directions for use of the additive and premixtures, the storage 
conditions and stability to heat treatment shall be indicate d.
3.On the label of the additive the following shall be indicated :
‘Recommended maximum content of the active substance per kg of 
compl ete feedingstuff with a moisture cont ent of 12 %:
— mar ine animals: 0,05 mg;
— cats: 1 mg;
— other species or categor ies of animals: 5 mg.’
4.The functional group, the identif ication number , the name and the 
added amount of the active substance shall be indicated on the label 
of the premixture where the use level on the label of the premixture 
would result in exceed ing the level refer red to in point 3.
5.For users of the additive and premixtures, feed business operat ors 
shall establish operational procedures and organisational measures 
to address poten tial risks by inhalation, dermal contact or eyes 
contact. Where those risks cannot be eliminated or reduced to a 
minimum by such procedures and measures, the additive and 
premixtures shall be used with personal protective equipment, 
including skin, eye and breathing prote ction.9 August 2032
Analytical method (1)
For the identification of 
4-methyl acetophenone 
in the feed additive and in 
feed flavouri ng 
premixtures:
Gas chromat ography 
mass spectrometr y with 
retention time locking 
GC-MS-RTL.
(1)Details of the analytical methods are available at the following address of the Reference Laborato ry: https://joint-research-c entre.ec.europa.eu/eurl-fa-eurl-f eed-additives/eurl-fa-author isation/eurl-f a-evaluation- 
repor ts_enEN Offi cial Jour nal of the European Union L 191/28 20.7.2022  Identification 
number of 
the additiveAdditiveCompo sition, chemical 
formula, descr iption, 
analytical methodSpecies or 
categor y 
of animalMaxi -
mum 
ageMinimum 
conten tMaximum 
conten t
Other provisi onsEnd of period of 
author isationmg of additive/kg of 
complet e feedingstuff 
with a moisture content of 
12 %
Categor y: Sensor y additiv es
Functional group: Flavour ing compounds
2b07038 4-Metho x-
yaceto phe-
noneAdditive composition
4-Metho xyacetophenone
Char acter isation of active 
substance
4-Metho xyacetophenone
Produced by chemical 
synthesis
Purity: > 97 %
Chemical formula: 
C9H10O2
CAS number: 100-06-1
FLAVIS: 07.038All 
animal 
species- - - 1.The additive shall be incor porate d into the feed in the form of a 
premixture.
2.In the directions for use of the additive and premixtures, the storage 
conditions and stability to heat treatment shall be indicate d.
3.On the label of the additive the following shall be indicated :
‘Recommended maximum content of the active substance per kg of 
compl ete feedingstuff with a moisture cont ent of 12 %:
— mar ine animals: 0,05 mg;
— cats: 1 mg;
— other species or categor ies of animals: 5 mg.’
4.The functional group, the identif ication number , the name and the 
added amount of the active substance shall be indicated on the label 
of the premixture where the use level on the label of the premixture 
would result in exceed ing the level refer red to in point 3.
5.For users of the additive and premixtures, feed business operat ors 
shall establish operational procedures and organisational measures 
to address poten tial risks by inhalation, dermal contact or eyes 
contact. Where those risks cannot be eliminated or reduced to a 
minimum by such procedures and measures, the additive and 
premixtures shall be used with personal protective equipment, 
including skin, eye and breathing prote ction.9 August 2032
Analytical method (1)
For the identification of 
4-metho xyacet ophenone 
in the feed additive and in 
feed flavouri ng 
premixtures:
Gas chromat ography 
mass spectrometr y with 
retention time locking 
GC-MS-RTL.
(1)Details of the analytical methods are available at the following address of the Reference Laborato ry: https://joint-research-c entre.ec.europa.eu/eurl-fa-eurl-f eed-additives/eurl-fa-author isation/eurl-f a-evaluation- 
repor ts_enEN Offi cial Jour nal of the European Union 20.7.2022 L 191/29  Identification 
number of 
the additiveAdditiveCompo sition, chemical 
formula, descr iption, 
analytical methodSpecies or 
categor y 
of animalMaxi -
mum 
ageMinimum 
conten tMaximum 
conten t
Other provisi onsEnd of period of 
author isationmg of additive/kg of 
complet e feedingstuff 
with a moisture content of 
12 %
Categor y: Sensor y additiv es
Functional group: Flavour ing compounds
2b04026 3-Methy l-
phenolAdditive composition
3-Methy lphenol
Char acter isation of active 
substance
3-Methy lphenol
Produced by chemical 
synthesis
Purity: 98 %
Chemical formula: 
C7H8O
CAS number: 108-39-4
FLAVIS: 04.026All 
animal 
species- - - 1.The additive shall be incor porate d into the feed in the form of a 
premixture.
2.In the directions for use of the additive and premixtures, the storage 
conditions and stability to heat treatment shall be indicate d.
3.On the label of the additive the following shall be indicated :
‘Recommended maximum content of the active substance per kg of 
compl ete feedingstuff with a moisture cont ent of 12 %: 5 mg.’
4.The functional group, the identif ication number , the name and the 
added amount of the active substance shall be indicated on the label 
of the premixture where the use level on the label of the premixture 
would result in exceed ing the level refer red to in point 3.
5.For users of the additive and premixtures, feed business operat ors 
shall establish operational procedures and organisational measures 
to address poten tial risks by inhalation, dermal contact or eyes 
contact. Where those risks cannot be eliminated or reduced to a 
minimum by such procedures and measures, the additive and 
premixtures shall be used with personal protective equipment, 
including skin, eye and breathing prote ction.9 August 2032
Analytical method (1)
For the identification of 
3-methyl phenol in the 
feed additive and in feed 
flavouring premixtures:
Gas chromat ography 
mass spectrometr y with 
retention time locking 
GC-MS-RTL.
(1)Details of the analytical methods are available at the following address of the Reference Laborato ry: https://joint-research-c entre.ec.europa.eu/eurl-fa-eurl-f eed-additives/eurl-fa-author isation/eurl-f a-evaluation- 
repor ts_enEN Offi cial Jour nal of the European Union L 191/30 20.7.2022  Identification 
number of 
the additiveAdditiveCompo sition, chemical 
formula, descr iption, 
analytical methodSpecies or 
categor y 
of animalMaxi -
mum 
ageMinimum 
conten tMaximum 
conten t
Other provisi onsEnd of period of 
author isationmg of additive/kg of 
complet e feedingstuff 
with a moisture content of 
12 %
Categor y: Sensor y additiv es
Functional group: Flavour ing compounds
2b04048 3,4-Dime -
thyl phenolAdditive composition
3,4-Dimethylphenol
Char acter isation of active 
substance
3,4-Dimethylphenol
Produced by chemical 
synthesis
Purity: 98 %
Chemical formula: 
C8H10O
CAS number: 95-65-8
FLAVIS: 04.048All 
animal 
species- - - 1.The additive shall be incor porate d into the feed in the form of a 
premixture.
2.In the directions for use of the additive and premixtures, the storage 
conditions and stability to heat treatment shall be indicate d.
3.On the label of the additive the following shall be indicated :
‘Recommended maximum content of the active substance per kg of 
compl ete feedingstuff with a moisture cont ent of 12 %: 5 mg.’
4.The functional group, the identif ication number , the name and the 
added amount of the active substance shall be indicated on the label 
of the premixture where the use level on the label of the premixture 
would result in exceed ing the level refer red to in point 3.
5.For users of the additive and premixtures, feed business operat ors 
shall establish operational procedures and organisational measures 
to address poten tial risks by inhalation, dermal contact or eyes 
contact. Where those risks cannot be eliminated or reduced to a 
minimum by such procedures and measures, the additive and 
premixtures shall be used with personal protective equipment, 
including skin, eye and breathing prote ction.9 August 2032
Analytical method (1)
For the identification of 
3,4-dimethyl phenol in 
the feed additive and in 
feed flavouri ng 
premixtures:
Gas chromat ography 
mass spectrometr y with 
retention time locking 
GC-MS-RTL.
(1)Details of the analytical methods are available at the following address of the Reference Laborato ry: https://joint-research-c entre.ec.europa.eu/eurl-fa-eurl-f eed-additives/eurl-fa-author isation/eurl-f a-evaluation- 
repor ts_enEN Offi cial Jour nal of the European Union 20.7.2022 L 191/31  Identification 
number of 
the additiveAdditiveCompo sition, chemical 
formula, descr iption, 
analytical methodSpecies or 
categor y 
of animalMaxi -
mum 
ageMinimum 
conten tMaximum 
conten t
Other provisi onsEnd of period of 
author isationmg of additive/kg of 
complet e feedingstuff 
with a moisture content of 
12 %
Categor y: Sensor y additiv es
Functional group: Flavour ing compounds
2b04015 1-Metho xy- 
4-methyl -
benzeneAdditive composition
1-Metho xy- 
4-methyl benzene
Char acter isation of active 
substance
1-Metho xy- 
4-methyl benzene
Produced by chemical 
synthesis
Purity: 99 %
Chemical formula: 
C8H10O
CAS number: 104-93-8
FLAVIS: 04.015All 
animal 
species- - - 1.The additive shall be incor porate d into the feed in the form of a 
premixture.
2.In the directions for use of the additive and premixtures, the storage 
conditions and stability to heat treatment shall be indicate d.
3.On the label of the additive the following shall be indicated :
‘Recommended maximum content of the active substance per kg of 
compl ete feedingstuff with a moisture cont ent of 12 %: 1 mg.’
4.The functional group, the identif ication number , the name and the 
added amount of the active substance shall be indicated on the label 
of the premixture where the use level on the label of the premixture 
would result in exceed ing the level refer red to in point 3.
5.For users of the additive and premixtures, feed business operat ors 
shall establish operational procedures and organisational measures 
to address poten tial risks by inhalation, dermal contact or eyes 
contact. Where those risks cannot be eliminated or reduced to a 
minimum by such procedures and measures, the additive and 
premixtures shall be used with personal protective equipment, 
including skin, eye and breathing prote ction.9 August 2032
Analytical method (1)
For the identification of 
1-metho xy- 
4-methyl benzene in the 
feed additive and in feed 
flavouring premixtures:
Gas chromat ography 
mass spectrometr y with 
retention time locking 
GC-MS-RTL.
(1)Details of the analytical methods are available at the following address of the Reference Laborato ry: https://joint-research-c entre.ec.europa.eu/eurl-fa-eurl-f eed-additives/eurl-fa-author isation/eurl-f a-evaluation- 
repor ts_enEN Offi cial Jour nal of the European Union L 191/32 20.7.2022  Identification 
number of 
the additiveAdditiveCompo sition, chemical 
formula, descr iption, 
analytical methodSpecies or 
categor y 
of animalMaxi -
mum 
ageMinimum 
conten tMaximum 
conten t
Other provisi onsEnd of period of 
author isationmg of additive/kg of 
complet e feedingstuff 
with a moisture content of 
12 %
Categor y: Sensor y additiv es
Functional group: Flavour ing compounds
2b13169 Trimethy -
loxazoleAdditive composition
Trimethylo xazole
Char acter isation of active 
substance
Trimethylo xazole
Produced by chemical 
synthesis
Purity: > 95 %
Chemical formula: 
C6H9ON
CAS number: 
20662-84-4
FLAVIS: 13.169All 
animal 
species- - - 1.The additive shall be incor porate d into the feed in the form of a 
premixture.
2.In the directions for use of the additive and premixtures, the storage 
conditions and stability to heat treatment shall be indicate d.
3.On the label of the additive the following shall be indicated :
‘Recommended maximum content of the active substance per kg of 
compl ete feedingstuff with a moisture cont ent of 12 %:
— mar ine animals: 0,05 mg;
— poultr y and pigs: 0,3 mg;
— other species or categor ies of animals: 0,5 mg.’
4.The functional group, the identif ication number , the name and the 
added amount of the active substance shall be indicated on the label 
of the premixture where the use level on the label of the premixture 
would result in exceed ing the level refer red to in point 3.
5.For users of the additive and premixtures, feed business operat ors 
shall establish operational procedures and organisational measures 
to address poten tial risks by inhalation, dermal contact or eyes 
contact. Where those risks cannot be eliminated or reduced to a 
minimum by such procedures and measures, the additive and 
premixtures shall be used with personal protective equipment, 
including skin, eye and breathing prote ction.9 August 2032
Analytical method (1)
For the identification of 
trimethyl oxazole in the 
feed additive and in feed 
flavouring premixtures:
Gas chromat ography 
mass spectrometr y with 
retention time locking 
GC-MS-RTL.
(1)Details of the analytical methods are available at the following address of the Reference Laborato ry: https://joint-research-c entre.ec.europa.eu/eurl-fa-eurl-f eed-additives/eurl-fa-author isation/eurl-f a-evaluation- 
repor ts_enEN Offi cial Jour nal of the European Union 20.7.2022 L 191/33  Identification 
number of 
the additiveAdditiveCompo sition, chemical 
formula, descr iption, 
analytical methodSpecies or 
categor y 
of animalMaxi -
mum 
ageMinimum 
conten tMaximum 
conten t
Other provisi onsEnd of period of 
author isationmg of additive/kg of 
complet e feedingstuff 
with a moisture content of 
12 %
Categor y: Sensor y additiv es
Functional group: Flavour ing compounds
2b15012 4,5-Dih y-
drothio -
phen-3(2H)- 
oneAdditive composition
4,5-Dih ydrothiophen-3 
(2H)-one
Char acter isation of active 
substance
4,5-Dih ydrothiophen-3 
(2H)-one
Produced by chemical 
synthesis.
Purity: 97 %
Chemical formula: 
C4H6OS
CAS number: 1003-04-9
FLAVIS: 15.012All 
animal 
species- - - 1.The additive shall be incor porate d into the feed in the form of a 
premixture.
2.In the directions for use of the additive and premixtures, the storage 
conditions and stability to heat treatment shall be indicate d.
3.On the label of the additive the following shall be indicated :
‘Recommended maximum content of the active substance per kg of 
compl ete feedingstuff with a moisture cont ent of 12 %: 0,05 mg.’
4.The functional group, the identif ication number , the name and the 
added amount of the active substance shall be indicated on the label 
of the premixture where the use level on the label of the premixture 
would result in exceed ing the level refer red to in point 3.
5.For users of the additive and premixtures, feed business operat ors 
shall establish operational procedures and organisational measures 
to address poten tial risks by inhalation, dermal contact or eyes 
contact. Where those risks cannot be eliminated or reduced to a 
minimum by such procedures and measures, the additive and 
premixtures shall be used with personal protective equipment, 
including skin, eye and breathing prote ction.9 August 2032
Analytical method (1)
For the identification of 
4,5-dih ydrothiophen-3 
(2H)-one in the feed 
additive and in feed 
flavouring premixtures:
Gas chromat ography 
mass spectrometr y with 
retention time locking 
GC-MS-RTL.
(1)Details of the analytical methods are available at the following address of the Reference Laborato ry: https://joint-research-c entre.ec.europa.eu/eurl-fa-eurl-f eed-additives/eurl-fa-author isation/eurl-f a-evaluation- 
repor ts_enEN Offi cial Jour nal of the European Union L 191/34 20.7.2022