COMMISSION IMPLEMENTING REGUL ATION (EU) 2023/1341 
of 30 June 2023
concer ning the renewal of the author isation of the preparations of Lactiplantibacillus plantar um DSM 
12836, Lactiplantibacillus plantar um DSM 12837, Lentilactobacillus buch neri DSM 16774, Pediococcus 
acidilactici DSM 16243, Pediococcus pentosaceus DSM 12834, Lacticaseibacillus paracasei DSM 16245, 
Levilactobacillus brevis DSM 12835, Lacticaseibacillus rhamnosus NCIMB 30121, Lactococcus lactis 
NCIMB 30160, Lentilactobacillus buch neri DSM 12856 and Lactococcus lactis DSM 11037 as feed 
additiv es for all animal species, and repealing Implementing Regulation (EU) No 1263/2011 
(Text with EEA relevance) 
THE EUR OPEAN COMMISSION,
Havi ng regar d to the Treaty on the Functioning of the European Union,
Havi ng rega rd to Regulation (EC) No 1831/2003 of the European Parliament and of the Council of 22 Sept ember 2003 on 
additives for use in animal nutr ition (1), and in particular Article 9(2) thereof,
Whereas:
(1) Regulation (EC) No 1831/2003 provid es for the author isation of additives for use in animal nutr ition and for the 
grounds and procedures for granting and renewing such an author isation.
(2) The preparations of Lactiplantibacillus plantar um DSM 12836 (previously taxonomically identif ied as Lactobacillus 
plantar um DSM 12836), Lactiplantibacillus plantar um DSM 12837 (previously taxonomically identified as Lactobacillus 
plantar um DSM 12837), Lentilactobacillus buchner i DSM 16774 (previously taxonomically identif ied as Lactobacillus 
buchner i DSM 16774), Pediococcus acidilactici DSM 16243, Pediococcus pentosaceus DSM 12834, Lacticaseibacillus 
paracasei DSM 16245 (previously taxonomically identified as Lactobacillus paracasei DSM 16245), Levilactobacillus 
brevis DSM 12835 (previously taxonomically identified as Lactobacillus brevis DSM 12835), Lacticaseibacillus 
rhamnosus NCIMB 30121 (previously taxonomically identified as Lactobacillus rhamnosus NCIMB 30121), Lactococcus 
lactis NCIMB 30160, Lentilactobacillus buchner i DSM 12856 (previously taxonomically identif ied as Lactobacillus 
buchner i DSM 12856) and Lactococcus lactis DSM 11037 were author ised for a period of 10 years as feed additives 
for all animal species by Commission Implementing Regulation (EU) No 1263/2011 (2).
(3) In accordance with Article 14(1) of Regulation (EC) No 1831/2003, applications were submitted for the renewal of 
the author isation of the preparations of Lactiplantibacillus plantar um DSM 12836, Lactiplantibacillus plantar um DSM 
12837, Lentilactobacillus buchner i DSM 16774, Pediococcus acidilactici DSM 16243, Pediococcus pentosaceus DSM 
12834, Lacticaseibacillus paracasei DSM 16245, Levilactobacillus brevis DSM 12835, Lacticaseibacillus rhamnosus NCIMB 
30121, Lactococcus lactis NCIMB 30160, Lentilactobacillus buchner i DSM 12856 and Lactococcus lactis DSM 11037 as 
feed additives for all animal species, requesting the additives to be classified in the additive categor y ‘technological 
additives ’ and in the functional group ‘silage additives ’. Those applications were accompanied by the particulars and 
documents required under Article 14(2) of that Regulation.
(1)OJ L 268, 18.10.2003, p. 29.
(2)Commission Implementing Regulation (EU) No 1263/2011 of 5 December 2011 concer ning the author isation of Lactobacillus buchner i 
(DSM 16774), Lactobacillus buchner i (DSM 12856), Lactobacillus paracasei (DSM 16245), Lactobacillus paracasei (DSM 16773), Lactobacillus 
plantar um (DSM 12836), Lactobacillus plantar um (DSM 12837), Lactobacillus brevis (DSM 12835), Lactobacillus rhamnosus (NCIMB 
30121), Lactococcus lactis (DSM 11037), Lactococcus lactis (NCIMB 30160), Pediococcus acidilactici (DSM 16243) and Pediococcus 
pentosaceus (DSM 12834) as feed additives for all animal species (OJ L 322, 6.12.2011, p. 3).EN Offi cial Jour nal of the European Union 3.7.2023 L 168/3  (4) The European Food Safety Authority (‘the Author ity’) concluded in its opinions of 5 May 2021 (3),(4), 23 June 
2021 (5),(6),(7), 29 Sept ember 2021 (8),(9),(10), 10 November 2021 (11), 26 Januar y 2022 (12)and 23 March 
2022 (13)that the applicants have provided evidence that the additives remain safe for all animal species, the 
consumers and the environment under the author ised conditions of use. It also concluded that the preparations are 
not irritant to skin and eyes, but that they should be considered poten tial skin and respirato ry sensitisers.
(5) In accordance with Article 5(4), point (c), of Commission Regulation (EC) No 378/2005 (14), the Reference 
Laboratory set up by Regulation (EC) No 1831/2003 considered that the conclusions and recommendations 
reach ed in the previous assessments are valid and applicable for the current applications.
(6) The assessment of the preparations shows that the conditions for author isation, as provided for in Article 5 of 
Regulation (EC) No 1831/2003, are satisfied. According ly, the author isation of those additives should be renewed.
(7) The Commission considers that appropr iate protect ive measures should be taken to prevent adverse effects on the 
health of the users of the additive.
(8) As a consequence of the renewal of the author isation of those preparations as feed additives as well as the expir y of 
another author isation concer ned by Implementing Regulation (EU) No 1263/2011, that Regulation should be 
repealed.
(9) Since safet y reasons do not require the immediate application of the modifications to the conditions of author isation 
of the preparations of Lactiplantibacillus plantar um DSM 12836, Lactiplantibacillus plantar um DSM 12837, Lentilacto ­
bacillus buchner i DSM 16774, Lacticaseibacillus paracasei DSM 16245, Levilactobacillus brevis DSM 12835, Lacticasei ­
bacillus rhamnosus NCIMB 30121 and Lentilactobacillus buchner i DSM 12856, it is appropr iate to provide for a 
transitional period for inter ested parties to prepare themselves to meet the new requirements resulting from the 
renewal of the author isation.
(10) The measures provid ed for in this Regulation are in accordance with the opinion of the Standing Committ ee on 
Plants, Animals, Food and Feed,
HAS ADOPTED THIS REGUL ATION:
Article 1
Renewal of author isation
The author isation of the preparations specifi ed in the Annex, belonging to the additive categor y ‘technological additives’ 
and to the functional group ‘silage additives ’, are renewed subject to the conditions laid down in that Annex.
(3)EFSA Journal 2021;19(6):6626.
(4)EFSA Journal 2021;19(6):6614.
(5)EFSA Journal 2021;19(7):6696.
(6)EFSA Journal 2021;19(7):6697.
(7)EFSA Journal 2021;19(7):6713.
(8)EFSA Journal 2021;19(11):6902.
(9)EFSA Journal 2021;19(11):6900.
(10)EFSA Journal 2021;19(11):6901.
(11)EFSA Journal 2022;20(1):6975.
(12)EFSA Journal 2022;20(2):7148.
(13)EFSA Journal 2022;20(4):7241.
(14)Commission Regulation (EC) No 378/2005 of 4 March 2005 on detailed rules for the implementation of Regulation (EC) 
No 1831/2003 of the European Parliament and of the Council as regards the duties and tasks of the Community Reference 
Laboratory concer ning applications for author isations of feed additives (OJ L 59, 5.3.2005, p. 8).EN Offi cial Jour nal of the European Union L 168/4 3.7.2023  Article 2
Repeal
Implementing Regulation (EU) No 1263/2011 is repealed.
Article 3
Transitional measures
The preparations of Lactiplantibacillus plantar um DSM 12836, Lactiplantibacillus plantar um DSM 12837, Lentilactobacillus 
buchner i DSM 16774, Lacticaseibacillus paracasei DSM 16245, Levilactobacillus brevis DSM 12835, Lacticaseibacillus rhamnosus 
NCIMB 30121 and Lentilactobacillus buchner i DSM 12856 specifi ed in the Annex and feed containing them, whic h are 
produced and labelled before 23 July 2024 in accordance with the rules applicable before 23 July 2023 may continue to 
be placed on the market and used until the existing stoc ks are exhausted.
Article 4
Entr y into force
This Regulation shall enter into force on the twentieth day followi ng that of its publication in the Official Journal of the 
European Union.
This Regulation shall be binding in its entirety and directly applicable in all Member States.
Done at Brussels, 30 June 2023 .
For the Commission
The President
Ursula VON DER LEYENEN Offi cial Jour nal of the European Union 3.7.2023 L 168/5  ANNEX 
Identifica -
tion 
number of 
the additiveAdditiveCompo sition, chem ical formula, descr iption, 
analytical methodSpecies or 
categor y of 
animalMaxi -
mum 
ageMini -
mum 
contentMaxi -
mum 
content
Other provisionsEnd of period of 
author isation
CFU/kg of fresh 
material
Categor y: technological additiv es. Functional group: silage additiv es
1k2078 Lactiplantibacillus 
plantar um DSM 
12836Additive composition:
Preparation of Lactiplantibacillus plantar um DSM 
12836 containing a minimum of 5 × 1011 CFU/g 
additiveAll animal 
species- - - 1.In the directions for use of the additive and 
premixtures, the stora ge conditions shall be 
indicated .
2.Minimum content of the additive when used 
without combination with other micro-or ­
gani sms as silage additives: 1×108 CFU/kg 
fresh mate rial.
3.For users of the additive and premixtures, 
feed business operat ors shall establish opera ­
tional procedures and orga nisational mea ­
sures to address poten tial risks resulting 
from their use. Where those risks cannot 
be eliminated by such procedures and mea ­
sures, the additive and premixtures shall be 
used with personal skin and breathing pro­
tective equipment.23.7.2033
Char acter isation of the active substance:
Viable cells of Lactiplantibacillus plantar um DSM 
12836
Analytical method (1)
Enumeration in the feed additive of 
Lactiplantibacillus plantar um DSM 12836:
— Spread plate method using MRS agar (EN 
15787)
Identif ication of Lactiplantibacillus plantar um 
DSM 12836:
— Pulsed-Field Gel Electrophoresis (PFGE) or 
DNA sequencing methods
(1)Details of the analytical methods are available at the following address of the Reference Laborato ry: https://joint-research-c entre.ec.europa.eu/eurl-fa-eurl-f eed-additives/eurl-fa-author isation/eurl-f a-evaluation- 
repor ts_enEN Offi cial Jour nal of the European Union L 168/6 3.7.2023  Identifica -
tion 
number of 
the additiveAdditiveCompo sition, chem ical formula, descr iption, 
analytical methodSpecies or 
categor y of 
animalMaxi -
mum 
ageMini -
mum 
contentMaxi -
mum 
content
Other provisionsEnd of period of 
author isation
CFU/kg of fresh 
material
Categor y: technological additiv es. Functional group: silage additiv es
1k2079 Lactiplantibacillus 
plantar um DSM 
12837Additive composition:
Preparation of Lactiplantibacillus plantar um DSM 
12837 containing a minimum of 5 × 1011 CFU/g 
additiveAll animal 
species- - - 1.In the directions for use of the additive and 
premixtures, the stora ge conditions shall be 
indicated .
2.Minimum content of the additive when used 
without combination with other micro-or ­
gani sms as silage additives: 1×108 CFU/kg 
fresh mate rial.
3.For users of the additive and premixtures, 
feed business operat ors shall establish opera ­
tional procedures and orga nisational mea ­
sures to address poten tial risks resulting 
from their use. Where those risks cannot 
be eliminated by such procedures and mea ­
sures, the additive and premixtures shall be 
used with personal skin and breathing pro­
tective equipment.23.7.2033
Char acter isation of the active substance:
Viable cells of Lactiplantibacillus plantar um DSM 
12837
Analytical method (1)
Enumeration in the feed additive of 
Lactiplantibacillus plantar um DSM 12837:
— Spread plate method using MRS agar (EN 
15787)
Identif ication of Lactiplantibacillus plantar um 
DSM 12837:
— Pulsed-Field Gel Electrophoresis (PFGE) or 
DNA sequencing methods
(1)Details of the analytical methods are available at the following address of the Reference Laborato ry: https://joint-research-c entre.ec.europa.eu/eurl-fa-eurl-f eed-additives/eurl-fa-author isation/eurl-f a-evaluation- 
repor ts_enEN Offi cial Jour nal of the European Union 3.7.2023 L 168/7  Identifica -
tion 
number of 
the additiveAdditiveCompo sition, chem ical formula, descr iption, 
analytical methodSpecies or 
categor y of 
animalMaxi -
mum 
ageMini -
mum 
contentMaxi -
mum 
content
Other provisionsEnd of period of 
author isation
CFU/kg of fresh 
material
Categor y: technological additiv es. Functional group: silage additiv es
1k2074 Lentilactobacillus 
buchner i DSM 
16774Additive composition:
Preparation of Lentilactobacillus buchner i DSM 
16774 containing a minimum of 5 × 1011 CFU/g 
additiveAll animal 
species- - - 1.In the directions for use of the additive and 
premixtures, the stora ge conditions shall be 
indicated .
2.Minimum content of the additive when used 
without combination with other micro-or ­
gani sms as silage additives: 1×108 CFU/kg 
fresh mate rial.
3.For users of the additive and premixtures, 
feed business operat ors shall establish opera ­
tional procedures and orga nisational mea ­
sures to address poten tial risks resulting 
from their use. Where those risks cannot 
be eliminated by such procedures and mea ­
sures, the additive and premixtures shall be 
used with personal skin and breathing pro­
tective equipment.23.7.2033
Char acter isation of the active substance:
Viable cells of Lentilactobacillus buchner i DSM 
16774
Analytical method (1)
Enumeration in the feed additive of 
Lentilactobacillus buchner i DSM 16774:
— Spread plate method using MRS agar (EN 
15787)
Identif ication of Lentilactobacillus buchner i DSM 
16774:
— Pulsed-Field Gel Electrophoresis (PFGE) or 
DNA sequencing methods
(1)Details of the analytical methods are available at the following address of the Reference Laborato ry: https://joint-research-c entre.ec.europa.eu/eurl-fa-eurl-f eed-additives/eurl-fa-author isation/eurl-f a-evaluation- 
repor ts_enEN Offi cial Jour nal of the European Union L 168/8 3.7.2023  Identifica -
tion 
number of 
the additiveAdditiveCompo sition, chem ical formula, descr iption, 
analytical methodSpecies or 
categor y of 
animalMaxi -
mum 
ageMini -
mum 
contentMaxi -
mum 
content
Other provisionsEnd of period of 
author isation
CFU/kg of fresh 
material
Categor y: technological additiv es. Functional group: silage additiv es
1k2102 Pediococcus 
acidilactici DSM 
16243Additive composition:
Preparation of Pediococcus acidilactici DSM 16243 
containing a minimum of 5 × 1011 CFU/g 
additiveAll animal 
species- - - 1.In the directions for use of the additive and 
premixtures, the stora ge conditions shall be 
indicated .
2.Minimum content of the additive when used 
without combination with other micro-or ­
gani sms as silage additives: 1×108 CFU/kg 
fresh mate rial.
3.For users of the additive and premixtures, 
feed business operat ors shall establish opera ­
tional procedures and orga nisational mea ­
sures to address poten tial risks resulting 
from their use. Where those risks cannot 
be eliminated by such procedures and mea ­
sures, the additive and premixtures shall be 
used with personal skin and breathing pro­
tective equipment.23.7.2033
Char acter isation of the active substance:
Viable cells of Pediococcus acidilactici DSM 16243
Analytical method (1)
Enumeration in the feed additive of Pediococcus 
acidilactici DSM 16243:
— Spread plate method using MRS agar (EN 
15786)
Identif ication of Pediococcus acidilactici DSM 
16243:
— Pulsed-Field Gel Electrophoresis (PFGE) or 
DNA sequencing methods
(1)Details of the analytical methods are available at the following address of the Reference Laborato ry: https://joint-research-c entre.ec.europa.eu/eurl-fa-eurl-f eed-additives/eurl-fa-author isation/eurl-f a-evaluation- 
repor ts_enEN Offi cial Jour nal of the European Union 3.7.2023 L 168/9  Identifica -
tion 
number of 
the additiveAdditiveCompo sition, chem ical formula, descr iption, 
analytical methodSpecies or 
categor y of 
animalMaxi -
mum 
ageMini -
mum 
contentMaxi -
mum 
content
Other provisionsEnd of period of 
author isation
CFU/kg of fresh 
material
Categor y: technological additiv es. Functional group: silage additiv es
1k2103 Pediococcus 
pentosaceus DSM 
12834Additive composition:
Preparation of Pediococcus pentosaceus DSM 
12834 containing a minimum of 4 × 1011 CFU/g 
additiveAll animal 
species- - - 1.In the directions for use of the additive and 
premixtures, the stora ge conditions shall be 
indicated .
2.Minimum content of the additive when used 
without combination with other micro-or ­
gani sms as silage additives: 1×108 CFU/kg 
fresh mate rial.
3.For users of the additive and premixtures, 
feed business operat ors shall establish opera ­
tional procedures and orga nisational mea ­
sures to address poten tial risks resulting 
from their use. Where those risks cannot 
be eliminated by such procedures and mea ­
sures, the additive and premixtures shall be 
used with personal skin and breathing pro­
tective equipment.23.7.2033
Char acter isation of the active substance:
Viable cells of Pediococcus pentosaceus DSM 12834
Analytical method (1)
Enumeration in the feed additive of Pediococcus 
pentosaceus DSM 12834:
— Spread plate method using MRS agar (EN 
15786)
— Identif ication of Pediococcus pentosaceus DSM 
12834:
Pulsed-Field Gel Electrophoresis (PFGE) or DNA 
sequencing methods
(1)Details of the analytical methods are available at the following address of the Reference Laborato ry: https://joint-research-c entre.ec.europa.eu/eurl-fa-eurl-f eed-additives/eurl-fa-author isation/eurl-f a-evaluation- 
repor ts_enEN Offi cial Jour nal of the European Union L 168/10 3.7.2023  Identifica -
tion 
number of 
the additiveAdditiveCompo sition, chem ical formula, descr iption, 
analytical methodSpecies or 
categor y of 
animalMaxi -
mum 
ageMini -
mum 
contentMaxi -
mum 
content
Other provisionsEnd of period of 
author isation
CFU/kg of fresh 
material
Categor y: technological additiv es. Functional group: silage additiv es
1k2076 Lacticaseibacillus 
paracasei DSM 
16245Additive composition:
Preparation of Lacticaseibacillus paracasei DSM 
16245 containing a minimum of 5 × 1011 CFU/g 
additiveAll animal 
species- - - 1.In the directions for use of the additive and 
premixtures, the stora ge conditions shall be 
indicated .
2.Minimum content of the additive when used 
without combination with other micro-or ­
gani sms as silage additives: 1×108 CFU/kg 
fresh mate rial.
3.For users of the additive and premixtures, 
feed business operat ors shall establish opera ­
tional procedures and orga nisational mea ­
sures to address poten tial risks resulting 
from their use. Where those risks cannot 
be eliminated by such procedures and mea ­
sures, the additive and premixtures shall be 
used with personal skin and breathing pro­
tective equipment.23.7.2033
Char acter isation of the active substance:
Viable cells of Lacticaseibacillus paracasei DSM 
16245
Analytical method (1)
Enumeration in the feed additive of 
Lacticaseibacillus paracasei DSM 16245:
— Spread plate method using MRS agar (EN 
15787)
Identif ication of Lacticaseibacillus paracasei DSM 
16245:
— Pulsed-Field Gel Electrophoresis (PFGE) or 
DNA sequencing methods
(1)Details of the analytical methods are available at the following address of the Reference Laborato ry: https://joint-research-c entre.ec.europa.eu/eurl-fa-eurl-f eed-additives/eurl-fa-author isation/eurl-f a-evaluation- 
repor ts_enEN Offi cial Jour nal of the European Union 3.7.2023 L 168/11  Identifica -
tion 
number of 
the additiveAdditiveCompo sition, chem ical formula, descr iption, 
analytical methodSpecies or 
categor y of 
animalMaxi -
mum 
ageMini -
mum 
contentMaxi -
mum 
content
Other provisionsEnd of period of 
author isation
CFU/kg of fresh 
material
Categor y: technological additiv es. Functional group: silage additiv es
1k20710 Levilactobacillus 
brevis DSM 12835Additive composition:
Preparation of Levilactobacillus brevis DSM 12835 
containing a minimum of 5 × 1011 CFU/g 
additiveAll animal 
species- - - 1.In the directions for use of the additive and 
premixtures, the stora ge conditions shall be 
indicated .
2.Minimum content of the additive when used 
without combination with other micro-or ­
gani sms as silage additives: 1×108 CFU/kg 
fresh mate rial.
3.For users of the additive and premixtures, 
feed business operat ors shall establish opera ­
tional procedures and orga nisational mea ­
sures to address poten tial risks resulting 
from their use. Where those risks cannot 
be eliminated by such procedures and mea ­
sures, the additive and premixtures shall be 
used with personal skin and breathing pro­
tective equipment.23.7.2033
Char acter isation of the active substance:
Viable cells of Levilactobacillus brevis DSM 12835
Analytical method (1)
Enumeration in the feed additive of 
Levilactobacillus brevis DSM 12835:
— Spread plate method using MRS agar (EN 
15787)
Identif ication of Levilactobacillus brevis DSM 
12835:
— Pulsed-Field Gel Electrophoresis (PFGE) or 
DNA sequencing methods
(1)Details of the analytical methods are available at the following address of the Reference Laborato ry: https://joint-research-c entre.ec.europa.eu/eurl-fa-eurl-f eed-additives/eurl-fa-author isation/eurl-f a-evaluation- 
repor ts_enEN Offi cial Jour nal of the European Union L 168/12 3.7.2023  Identifica -
tion 
number of 
the additiveAdditiveCompo sition, chem ical formula, descr iption, 
analytical methodSpecies or 
categor y of 
animalMaxi -
mum 
ageMini -
mum 
contentMaxi -
mum 
content
Other provisionsEnd of period of 
author isation
CFU/kg of fresh 
material
Categor y: technological additiv es. Functional group: silage additiv es
1k20711 Lacticaseibacillus 
rhamnosus NCIMB 
30121Additive composition:
Preparation of Lacticaseibacillus rhamnosus 
NCIMB 30121 containing a minimum of 4 × 
1011 CFU/g additiveAll animal 
species- - - 1.In the directions for use of the additive and 
premixtures, the stora ge conditions shall be 
indicated .
2.Minimum content of the additive when used 
without combination with other micro-or ­
gani sms as silage additives: 1×108 CFU/kg 
fresh mate rial.
3.For users of the additive and premixtures, 
feed business operat ors shall establish opera ­
tional procedures and orga nisational mea ­
sures to address poten tial risks resulting 
from their use. Where those risks cannot 
be eliminated by such procedures and mea ­
sures, the additive and premixtures shall be 
used with personal skin and breathing pro­
tective equipment.23.7.2033
Char acter isation of the active substance:
Viable cells of Lacticaseibacillus rhamnosus NCIMB 
30121.
Analytical method (1)
Enumeration in the feed additive of 
Lacticaseibacillus rhamnosus NCIMB 30121:
— Spread plate method using MRS agar (EN 
15787)
Identif ication of Lacticaseibacillus rhamnosus 
NCIMB 30121:
— Pulsed-Field Gel Electrophoresis (PFGE) or 
DNA sequencing methods
(1)Details of the analytical methods are available at the following address of the Reference Laborato ry: https://joint-research-c entre.ec.europa.eu/eurl-fa-eurl-f eed-additives/eurl-fa-author isation/eurl-f a-evaluation- 
repor ts_enEN Offi cial Jour nal of the European Union 3.7.2023 L 168/13  Identifica -
tion 
number of 
the additiveAdditiveCompo sition, chem ical formula, descr iption, 
analytical methodSpecies or 
categor y of 
animalMaxi -
mum 
ageMini -
mum 
contentMaxi -
mum 
content
Other provisionsEnd of period of 
author isation
CFU/kg of fresh 
material
Categor y: technological additiv es. Functional group: silage additiv es
1k2082 Lactococcus lactis 
NCIMB 30160Additive composition:
Preparation of Lactococcus lactis NCIMB 30160 
containing a minimum of 4 × 1011 CFU/g 
additiveAll animal 
species- - - 1.In the directions for use of the additive and 
premixtures, the stora ge conditions shall be 
indicated .
2.Minimum content of the additive when used 
without combination with other micro-or ­
gani sms as silage additives: 1×108 CFU/kg 
fresh mate rial.
3.If polyet hylene glycol (PEG 4000) is used as a 
cryoprotect ant, it shall be used up to a max ­
imum concentration of 0,025 mg/kg silage.
4.For users of the additive and premixtures, 
feed business operat ors shall establish opera ­
tional procedures and orga nisational mea ­
sures to address poten tial risks resulting 
from their use. Where those risks cannot 
be eliminated by such procedures and mea ­
sures, the additive and premixtures shall be 
used with personal skin and breathing pro­
tective equipment.23.7.2033
Char acter isation of the active substance:
Viable cells of Lactococcus lactis NCIMB 30160
Analytical method (1)
Enumeration in the feed additive of Lactococcus 
lactis NCIMB 30160:
— Pour plate method using MRS agar (ISO 
15214)
Identif ication of Lactococcus lactis NCIMB 30160:
— Pulsed-Field Gel Electrophoresis (PFGE) or 
DNA sequencing methods
(1)Details of the analytical methods are available at the following address of the Reference Laborato ry: https://joint-research-c entre.ec.europa.eu/eurl-fa-eurl-f eed-additives/eurl-fa-author isation/eurl-f a-evaluation- 
repor ts_enEN Offi cial Jour nal of the European Union L 168/14 3.7.2023  Identifica -
tion 
number of 
the additiveAdditiveCompo sition, chem ical formula, descr iption, 
analytical methodSpecies or 
categor y of 
animalMaxi -
mum 
ageMini -
mum 
contentMaxi -
mum 
content
Other provisionsEnd of period of 
author isation
CFU/kg of fresh 
material
Categor y: technological additiv es. Functional group: silage additiv es
1k2075 Lentilactobacillus 
buchner i DSM 
12856Additive composition:
Preparation of Lentilactobacillus buchner i DSM 
12856 containing a minimum of 5 × 1011 CFU/g 
additiveAll animal 
species- - - 1.In the directions for use of the additive and 
premixtures, the stora ge conditions shall be 
indicated .
2.Minimum content of the additive when used 
without combination with other micro-or ­
gani sms as silage additives: 1×108 CFU/kg 
fresh mate rial.
3.For users of the additive and premixtures, 
feed business operat ors shall establish opera ­
tional procedures and orga nisational mea ­
sures to address poten tial risks resulting 
from their use. Where those risks cannot 
be eliminated by such procedures and mea ­
sures, the additive and premixtures shall be 
used with personal skin and breathing pro­
tective equipment.23.7.2033
Char acter isation of the active substance:
Viable cells of Lentilactobacillus buchner i DSM 
12856
Analytical method (1)
Enumeration in the feed additive of 
Lentilactobacillus buchner i DSM 12856:
— Spread plate method using MRS agar (EN 
15787)
Identif ication of Lentilactobacillus buchner i DSM 
12856:
— Pulsed-Field Gel Electrophoresis (PFGE) or 
DNA sequencing methods
(1)Details of the analytical methods are available at the following address of the Reference Laborato ry: https://joint-research-c entre.ec.europa.eu/eurl-fa-eurl-f eed-additives/eurl-fa-author isation/eurl-f a-evaluation- 
repor ts_enEN Offi cial Jour nal of the European Union 3.7.2023 L 168/15  Identifica -
tion 
number of 
the additiveAdditiveCompo sition, chem ical formula, descr iption, 
analytical methodSpecies or 
categor y of 
animalMaxi -
mum 
ageMini -
mum 
contentMaxi -
mum 
content
Other provisionsEnd of period of 
author isation
CFU/kg of fresh 
material
Categor y: technological additiv es. Functional group: silage additiv es
1k2081 Lactococcus lactis 
DSM 11037Additive composition:
Preparation of Lactococcus lactis DSM 11037 
containing a minimum of 5 × 1010 CFU/g 
additiveAll animal 
species- - - 1.In the directions for use of the additive and 
premixtures, the stora ge conditions shall be 
indicated .
2.Minimum content of the additive when used 
without combination with other micro-or ­
gani sms as silage additives: 1×108 CFU/kg 
fresh mate rial.
3.For users of the additive and premixtures, 
feed business operat ors shall establish opera ­
tional procedures and orga nisational mea ­
sures to address poten tial risks resulting 
from their use. Where those risks cannot 
be eliminated by such procedures and mea ­
sures, the additive and premixtures shall be 
used with personal skin and breathing pro­
tective equipment.23.7.2033
Char acter isation of the active substance:
Viable cells of Lactococcus lactis DSM 11037
Analytical method (1)
Enumeration in the feed additive of Lactococcus 
lactis DSM 11037:
— Pour plate method using MRS agar (ISO 
15214)
Identif ication of Lactococcus lactis DSM 11037:
— Pulsed-Field Gel Electrophoresis (PFGE) or 
DNA sequencing methods
(1)Details of the analytical methods are available at the following address of the Reference Laborato ry: https://joint-research-c entre.ec.europa.eu/eurl-fa-eurl-f eed-additives/eurl-fa-author isation/eurl-f a-evaluation- 
repor ts_enEN Offi cial Jour nal of the European Union L 168/16 3.7.2023