COMMISSION IMPLEMENTING REGUL ATION (EU) 2022/1493 
of 8 September 2022
concer ning the author isation of L-methionine produced by Cor ynebacter ium glutamicum KCCM 
80245 and Escherichia coli KCCM 80246 as feed additiv es for all animal species 
(Text with EEA relevance) 
THE EUR OPEAN COMMISSION,
Havi ng regar d to the Treaty on the Functioning of the European Union,
Havi ng rega rd to Regulation (EC) No 1831/2003 of the European Parliament and of the Council of 22 Sept ember 2003 on 
additives for use in animal nutr ition (1), and in particular Article 9(2) thereof,
Whereas:
(1) Regulation (EC) No 1831/2003 provides for the author isation of additives for use in animal nutr ition.
(2) In accordance with Article 7 of Regulation (EC) No 1831/2003 an application was submitted for the author isation 
of L-met hionine produced by Corynebacter ium glutamicum KCCM 80245 and Escher ichia coli KCCM 80246. That 
application was accompanied by the particulars and documents required under Article 7(3) of that Regulation.
(3) The application concer ns the author isation of L-methionine produced by Corynebacter ium glutamicum KCCM 80245 
and Escher ichia coli KCCM 80246 as feed additives for all animal species to be classified in the additive catego ry 
‘nutr itional additives ’.
(4) The European Food Safety Author ity (‘the Author ity’) concluded in its opinion of 23 March 2022 (2)that, under the 
proposed conditions of use, neither of the two products of L-methionine produced by Corynebacter ium glutamicum 
KCCM 80245 and Escher ichia coli KCCM 80246 has an adverse effect on animal health, consumer health or the 
environment. As regards the safety of the user of that substance, the Author ity concluded that the substance, in 
either products, is not an irritant to skin/e yes nor a dermal sensitiser and shows no toxicity by inhalation. However , 
the product L-methionine ≥ 90 % presents a risk to the user consider ing the exposure to endoto xins by inhalation. 
Theref ore, the Commission considers that appropr iate prote ctive measures should be take n to prevent adverse 
effects on human health, in particular as regards the users of that form of the substance.
(5) The Auth ority further concluded that L-met hionine produced by Corynebacter ium glutamicum KCCM 80245 and 
Escher ichia coli KCCM 80246 is an effective source of methionine for all animal species and that in order to be as 
efficacious in ruminants as in non-r uminant species, the substance should be protect ed against degradation in the 
rumen. The Author ity does not consider that there is a need for specif ic requirements of post-market monitoring. It 
also verified the repor t on the method of analysis of the feed additives in feed submitted by the Reference Laborat ory 
set up by Regulation (EC) No 1831/2003.
(6) The assessment of this substance shows that the conditions for author isation, as provided for in Article 5 of 
Regulation (EC) No 1831/2003, are satisfied. According ly, the use of this substance should be author ised as 
specifi ed in the Annex to this Regulation.
(7) The measures provid ed for in this Regulation are in accordance with the opinion of the Standing Committ ee on 
Plants, Animals, Food and Feed,
(1)OJ L 268, 18.10.2003, p. 29.
(2)EFSA Jour nal 2022;20(4):7247.EN Offi cial Jour nal of the European Union L 234/18 9.9.2022  HAS ADOPTED THIS REGUL ATION:
Article 1
The substances specified in the Annex, belonging to the additive categor y ‘nutr itional additives ’ and to the functional group 
‘amino acids, their salts and analogues’ are author ised as feed additives in animal nutr ition, subject to the conditions laid 
down in that Annex.
Article 2
This Regulation shall enter into force on the twentieth day followi ng that of its publication in the Official Journal of the 
European Union.
This Regulation shall be binding in its entirety and directly applicable in all Member States.
Done at Brussels, 8 Sept ember 2022 .
For the Commission
The President
Ursula VON DER LEYENEN Offi cial Jour nal of the European Union 9.9.2022 L 234/19  ANNEX 
Identifica -
tion 
number of 
the additiveName of 
the 
holder of 
author i-
sationAdditiveComposition, chemical formula, descr iption, 
analytical method.Species or 
categor y 
of animalMaximum 
ageMini-mum 
contentMaxi-mum 
conten t
Other provisionsEnd of period 
of 
author isationmg/kg of com plete feed 
with a moisture conten t 
of 12 %
Categor y of nutr itional additiv es. Functional group: amino acids, their salts and analogues
3c305 - L-met hionine Additive composition
L-methionine with a minimum conte nt of 
98,5 % and a maximum moisture conte nt of 
0,5 %
Powder formAll 
species- - - 1.L-met hionine may be used via wate r 
for drinking.
2.The labelling of the additive and pre­
mixtures shall indicate the following:
‘The supplementation with 
L-met hionine, in particular via wate r 
for drinking, should take into account 
all essential and conditionally 
essential amino acids in order to avoid 
imbalances.’
3.In the directions for use of the addi ­
tive and premixture, the storage con­
ditions, the stability to heat treatment 
and the stability in water for drinking 
shall be indicate d.
4.The endoto xin cont ent of the additive 
and its dusting pote ntial shall ensure 
that the maximal endoto xin expo ­
sure of 1 600 IU endoto xins/m3 
air(3)is not exceed ed.29 Sept ember 
2032
Char acter isation of the activ e substance
L-methionine produced by fermentation with 
Corynebacter ium glutamicum KCCM 80245 and 
Escher ichia coli KCCM 80246
Chemical formula: C5H11NO 2S
CAS Number: 63-68-3.
Analytical methods (1)
For the determi nation of L-methionine in the 
feed additive:
—Food Chemical Codex ‘L-methionine mono ­
graph’ and
— Ion-exc hange chromatograph y coupled with 
post-column derivatisation and optical 
detection (IEC- VIS/FLD) – EN ISO 17180EN Offi cial Jour nal of the European Union L 234/20 9.9.2022  5.For users of the additive and premix ­
tures, feed business operators shall 
establish operational procedures 
and orga nisational measures to ad­
dress potential risks resulting from 
its use. Where those risks cannot be 
eliminated or reduced to a minimum 
by such procedures and measures, 
the additive and premixtures shall 
be used with personal prote ctive 
equipment including breathing pro­
tectio n.For the determi nation of methionine in 
premixtures
— Ion-exc hange chromatograph y coupled with 
post-column derivatisation and optical 
detection (IEC- VIS/FLD) – EN ISO 17180 
and
— Ion-exc hange chromatograph y coupled with 
post-column derivatisation and photo ­
metr ic detection (IEC- VIS), Commission 
Regulation (EC) No 152/2009 (2)(Annex 
III, F)
For the determi nation of methionine in 
compo und feed and feed mat erials:
— Ion-exc hange chromatograph y coupled with 
post-column derivatisation and photo ­
metr ic detection (IEC- VIS), Regulation (EC) 
No 152/2009 (Annex III, F)
For the determi nation of methionine in wate r:
— Ion-exc hange chromatograph y coupled with 
post-column derivatisation and photo ­
metr ic detec tion (IEC- VIS)
3c305ii - L-met hionine Additive composition
Preparation of L-methionine with a minimum 
cont ent of 90 % and a maximum moisture 
cont ent of 0,5 %
— other amino acids ≤ 0,63 %;
Powder formAll 
species- - - 1.L-met hionine may be used via wate r 
for drinking.
2.The labelling of the additive and pre­
mixtures shall indicate the following:
‘The supplementation with 
L-met hionine, in particular via wate r 
for drinking, should take into account 
all essential and conditionally 
essential amino acids in order to avoid 
imbalances.’29 Sept ember 
2032
Char acter isation of the activ e substance
L-methionine produced by fermentation with 
Corynebacter ium glutamicum KCCM 80245 and 
Escher ichia coli KCCM 80246EN Offi cial Jour nal of the European Union 9.9.2022 L 234/21  3.In the directions for use of the addi ­
tive and premixture, the storage con­
ditions, the stability to heat treatment 
and the stability in water for drinking 
shall be indicate d.
4.The endoto xin cont ent of the additive 
and its dusting pote ntial shall ensure 
that the maximal endoto xin expo ­
sure of 1 600 IU endoto xins/m3 
air(3)is not exceed ed.
5.For users of the additive and premix ­
tures, feed business operators shall 
establish operational procedures 
and orga nisational measures to ad­
dress potential risks resulting from 
its use. Where those risks cannot be 
eliminated or reduced to a minimum 
by such procedures and measures, 
the additive and premixtures shall 
be used with personal prote ctive 
equipment including breathing pro­
tectio n.Chemical formula: C5H11NO 2S
CAS Number: 63-68-3.
Analytical methods (1)
For the determi nation of L-methionine in the 
feed additive:
—Food Chemical Codex ‘L-methionine mono ­
graph’ and
— Ion-exc hange chromatograph y coupled with 
post-column derivatisation and optical 
detection (IEC- VIS/FLD) – EN ISO 17180
For the determi nation of methionine in 
premixtures
— Ion-exc hange chromatograph y coupled with 
post-column derivatisation and optical 
detection (IEC- VIS/FLD) – EN ISO 17180 
and
— Ion-exc hange chromatograph y coupled with 
post-column derivatisation and photo ­
metr ic detection (IEC- VIS), Regulation (EC) 
No 152/2009 (Annex III, F)
For the determi nation of methionine in 
compo und feed and feed mat erials:
— Ion-exc hange chromatograph y coupled with 
post-column derivatisation and photo ­
metr ic detection (IEC- VIS), Regulation (EC) 
No 152/2009 (Annex III, F)
For the determi nation of methionine in wate r:
— Ion-exc hange chromatograph y coupled with 
post-column derivatisation and photo ­
metr ic detec tion (IEC- VIS)
(1)Details of the analytical methods are available at the following address of the Reference Laborato ry: https://joint-research-c entre.ec.europa.eu/eurl-fa-eurl-f eed-additives/eurl-fa-author isation/eurl-f a-evaluation- 
repor ts_en
(2)Commission Regulation (EC) No 152/2009 of 27 Januar y 2009 laying down the methods of sampling and analysis for the official control of feed (OJ L 54, 26.2.2009, p. 1).
(3)Exposure calculated based on the endoto xin level and the dusting potential of the additive according to the method used by EFSA (EFSA Jour nal 2018;16(10):5458); analytical method: European 
Phar macopoeia 2.6.14. (bacterial endoto xins).EN Offi cial Jour nal of the European Union L 234/22 9.9.2022