COMMISSION IMPLEMENTING REGUL ATION (EU) 2022/1420 
of 22 August 2022
concer ning the author isation of L-glut amic acid and monosodium glutamate produced by 
Cor ynebacter ium glutamicum NITE BP-01681 as feed additiv es for all animal species 
(Text with EEA relevance) 
THE EUR OPEAN COMMISSION,
Havi ng regar d to the Treaty on the Functioning of the European Union,
Havi ng rega rd to Regulation (EC) No 1831/2003 of the European Parliament and of the Council of 22 Sept ember 2003 on 
additives for use in animal nutr ition (1), and in particular Article 9(2) thereof,
Whereas:
(1) Regulation (EC) No 1831/2003 provid es for the author isation of additives for use in animal nutr ition and for the 
grounds and procedures for granting such an author isation.
(2) In accordance with Article 7 of Regulation (EC) No 1831/2003, an application was submitted for the author isation 
of L-glutamic acid and monosodium glutamate produced by Corynebacter ium glutamicum NITE BP-01681. That 
application was accompanied by the particulars and documents required under Article 7(3) of Regulation (EC) 
No 1831/2003.
(3) The application concer ns the author isation of L-glutamic acid and monosodium glutamate produced by 
Corynebacter ium glutamicum NITE BP-01681 as feed additives for all animal species, to be classified in the catego ries 
‘nutr itional additives ’ and ‘sensor y additives’.
(4) The applicant request ed the feed additive to be author ised for use also in wate r for drinking. Howe ver, Regulation 
(EC) No 1831/2003 does not allow the author isation of ‘flavouring compo unds ’ for use in water for drinking. 
Theref ore, the use of monosodium glutamate produced by Corynebacter ium glutamicum KCCM 80188 in water for 
drinking should not be allowe d. The fact that the additive is not author ised for use as a flavouri ng in water for 
drinking does not preclude its use in compound feed administ ered via water .
(5) The European Food Safety Author ity (‘the Author ity’) concluded in its opinion of 26 Januar y 2022 (2)that, under the 
proposed conditions of use, L-glutamic acid and monosodium glutamate produced by Corynebacter ium glutamicum 
NITE BP-01681 do not have adverse effects on animal health, consumer safety or the envi ronment. It also 
concluded that these substances are neither considered irritant to skin or eyes, nor dermal sensitisers, but are 
considered a risk by inhalation. Theref ore, the Commission considers that appropr iate prot ective measures should 
be taken to prevent adverse effects on human health, in particular as regards the users of the additive. The Author ity 
also concluded that these substances have the poten tial to be efficacious as nutr itional additives and as flavouri ng 
compo unds in feedingstuffs. The Author ity does not consider that there is a need for specific requirements of post- 
mark et monitoring. It also verified the repor t on the methods of analysis of the feed additive in feed submitted by 
the Reference Laboratory set up by Regulation (EC) No 1831/2003.
(6) The assessment of L-glutamic acid and monosodium glutamate produced by Corynebacter ium glutamicum NITE 
BP-01681 shows that the conditions for author isation, as provided for in Article 5 of Regulation (EC) 
No 1831/2003, are satisfied. According ly, the use of these substances should be author ised as specifi ed in the 
Annex to this Regulation.
(1)OJ L 268, 18.10.2003, p. 29.
(2)EFSA Journal 2022;20(3):7156.EN Offi cial Jour nal of the European Union 23.8.2022 L 218/17  (7) Restr ictions and conditions should be provid ed for to allow better control. In particular , a recommended content 
should be indicated on the label of the feed additive. Where such content is exceeded, certain information should be 
indicated on the label of premixtures.
(8) The measures provid ed for in this Regulation are in accordance with the opinion of the Standing Committ ee on 
Plants, Animals, Food and Feed,
HAS ADOPTED THIS REGUL ATION:
Article 1
1. The substances specifi ed in the Annex, belonging to the additive categor y ‘nutr itional additives’ and to the functional 
group ‘amino acids, their salts and analogues’, are author ised as additives in animal nutr ition, subject to the conditions laid 
down in that Annex.
2. The substances specif ied in the Annex, belonging to the additive categor y ‘sensor y additives ’ and to the functional 
group ‘flavouri ng compo unds ’, are author ised as additives in animal nutr ition, subject to the conditions laid down in that 
Annex.
Article 2
This Regulation shall enter into force on the twentieth day followi ng that of its publication in the Official Journal of the 
European Union.
This Regulation shall be binding in its entirety and directly applicable in all Member States.
Done at Brussels, 22 August 2022 .
For the Commission
The President
Ursula VON DER LEYENEN Offi cial Jour nal of the European Union L 218/18 23.8.2022  ANNEX 
Identifi -
cation 
number 
of the 
additiveAdditiveComposition, chemical formula, descr iption, 
analytical method.Species or 
categ ory of 
animalMaxi -
mum 
ageMini -
mum 
conten tMaximum 
conten t
Other provisi onsEnd of period of 
author isationmg active  
substance/kg of 
complet e feed with a 
moisture content of 
12 %
Categor y: nutr itional additiv es.
Functional group: amino acids, their salts and analogues
2b620i L-Glutam ic acid Additive composition
L-Glutam ic acid
Char acter isation of the active substance
L-Glutam ic acid produced by Corynebacter ium 
glutamicum NITE BP-01681
Purity: ≥ 98 %
Chemical formula: C5H9O4N
CAS number: 56-86-0
Einecs number: 200-293-7
Analytical method (1)
For the identif ication of L-glutamic acid in the 
feed additive:
— Food Chemical Codex monographs: 
‘L-Glutamic acid’
For the quantification of glutamic acid in the 
feed additive:
— Ion-exc hang e chromat ography coupled 
with post-column derivatisation and visi­
ble or fluorescence detection 
(IEC- VIS/FLD) orAll animal 
species- - - 1.In the directions for use of the additive and 
premixture, the storage conditions and the 
stability to heat treatment shall be indicated.
2.The additive may be used via water for drink­
ing.
3.For users of the additive and premixtures, 
feed business operat ors shall establish opera ­
tional procedures and organisational mea ­
sures to address the poten tial risks by inhala ­
tion or dermal contact. Where those risks 
cannot be eliminated or reduced to a mini ­
mum by such procedures and measures, the 
additive and premixtures shall be used with 
appropr iate personal protect ive equipment, 
including breathing protection.
4.Declarations to be made on the labelling of 
the additive and premixtures: ‘The supple ­
mentation with L-glutamic acid, in particular 
via water for drinking, should take into ac­
count all essential and conditionally essential 
amino acids in order to avoid imbalances.’12 Sept ember 2032EN Offi cial Jour nal of the European Union 23.8.2022 L 218/19  — Ion-exc hang e chromat ography coupled 
with post-column derivatisation and 
photomet ric detection (IEC- VIS)
For the quantification of glutamic acid in 
premixtures:
— Ion-exc hang e chromat ography coupled 
with post-column derivatisation and visi­
ble or fluorescence detection 
(IEC- VIS/FLD) or
— Ion-exc hang e chromat ography coupled 
with post-column derivatisation and 
photomet ric detec tion (IEC- VIS), Com ­
mission Regulation (EC) No 152/2009 
(Annex III, F)
For the quantification of glutamic acid in 
feedingstuffs:
— Ion exchange chromat ography coupled 
to post-column derivatisation and photo ­
metr ic detec tion (IEC- VIS), Commission 
Regulation (EC) No 152/2009 (Annex III, 
F)
(1)Details of the analytical methods are available at the following address of the Reference Laborato ry: https://joint-research-c entre.ec.europa.eu/eurl-fa-eurl-f eed-additives/eurl-fa-author isation/eurl-f a-evaluation- 
repor ts_enEN Offi cial Jour nal of the European Union L 218/20 23.8.2022  Identifi -
cation 
number 
of the 
additiveAdditiveComposition, chemical formula, descr iption, 
analytical method.Species or 
categ ory of 
animalMaxi -
mum 
ageMini -
mum 
conten tMaximum 
conten t
Other provisi onsEnd of period of 
author isationmg active  
substance/kg of 
complet e feed with a 
moisture content of 
12 %
Categor y: Sensor y additiv es.
Functional group: Flav our ing compounds
2b620i L-Glutam ic acid Additive composition
L-Glutam ic acid
Char acter isation of the active substance
L-Glutam ic acid produced by Corynebacter ium 
glutamicum NITE BP-01681
Purity: ≥ 98 %
Chemical formula: C5H9O4N
CAS number: 56-86-0
Einecs number: 200-293-7
Analytical method (1)
For the identif ication of L-glutamic acid in the 
feed additive:
— Food Chemical Codex monographs: 
‘L-Glutamic acid’
For the quantification of glutamic acid in the 
feed additive:
— Ion-exc hang e chromat ography coupled 
with post-column derivatisation and visi­
ble or fluorescence detection 
(IEC- VIS/FLD) or
— Ion-exc hang e chromat ography coupled 
with post-column derivatisation and 
photomet ric detection (IEC- VIS)All animal 
species- - - 1.The additive shall be incor porate d into the 
feed in the form of a premixture.
2.In the directions for use of the additive and 
premixture, the storage conditions and the 
stability to heat treatment shall be indicated.
3.On the label of the additive the following 
shall be indicate d: ‘Recommended maxi ­
mum cont ent of the active substance of 
com plete feed with a moisture cont ent of 
12 %: 25 mg/kg.’
4.The functional group, the identification 
number , the name and the added amount 
of the active substance shall be indicate d on 
the label of the premixture, where the use 
level on the label of the premixture would 
result in exceed ing the level of active sub­
stance in compl ete feed referred to in point 3.
5.For users of the additive and premixtures, 
feed business operat ors shall establish opera ­
tional procedures and organisational mea ­
sures to address the poten tial risks by inhala ­
tion or dermal contact. Where those risks 
cannot be eliminated or reduced to a mini ­
mum by such procedures and measures, the 12 Sept ember 2032EN Offi cial Jour nal of the European Union 23.8.2022 L 218/21  For the quantification of glutamic acid in 
premixtures:
— Ion-exc hang e chromat ography coupled 
with post-column derivatisation and visi­
ble or fluorescence detection 
(IEC- VIS/FLD) or
— Ion-exc hang e chromat ography coupled 
with post-column derivatisation and 
photomet ric detec tion (IEC- VIS), Com ­
mission Regulation (EC) No 152/2009 
(Annex III, F)additive and premixtures shall be used with 
appropr iate personal protect ive equipment, 
including breathing protection.
(1)Details of the analytical methods are available at the following address of the Reference Laborato ry: https://joint-research-c entre.ec.europa.eu/eurl-fa-eurl-f eed-additives/eurl-fa-author isation/eurl-f a-evaluation- 
repor ts_enEN Offi cial Jour nal of the European Union L 218/22 23.8.2022  Identifi -
cation 
number 
of the 
additiveAdditiveComposition, chemical formula, descr iption, 
analytical method.Species or 
categ ory of 
animalMaxi -
mum 
ageMini -
mum 
conten tMaximum 
conten t
Other provisi onsEnd of period of 
author isationmg active  
substance/kg of 
complet e feed with a 
moisture content of 
12 %
Categor y: nutr itional additiv es.
Functional group: amino acids, their salts and analogues
2b621ii Monosodium 
glutamateAdditive composition
Monosodium glutamate
Char acter isation of the active substance
Monosodium glutamate produced by 
Corynebacter ium glutamicum NITE BP-01681
Purity: ≥ 99 %
Chemical formula: C5H8NaNO 4·H2O
CAS number: 6106-04-3
Einecs number: 205-538-1
Analytical method (1)
For the identification of monosodium 
L-glutamate monohydrat e in the feed 
additive:
— Food Chemical Codex monographs: 
‘Monosodium L-glutamat e’
For the quantification of monosodium 
glutamate in the feed additive:
— Ion-exc hang e chromat ography coupled 
with post-column derivatisation and visi­
ble or fluorescence detection 
(IEC- VIS/FLD) or
— Ion-exc hang e chromat ography coupled 
with post-column derivatisation and 
photomet ric detection (IEC- VIS)All animal 
species- - - 1.In the directions for use of the additive and 
premixture, the storage conditions and the 
stability to heat treatment shall be indicated.
2.The additive may be used via water for drink­
ing.
3.For users of the additive and premixtures, 
feed business operat ors shall establish opera ­
tional procedures and organisational mea ­
sures to address the poten tial risks by inhala ­
tion or dermal contact. Where those risks 
cannot be eliminated or reduced to a mini ­
mum by such procedures and measures, the 
additive and premixtures shall be used with 
appropr iate personal protect ive equipment, 
including breathing protection.
4.Declarations to be made on the labelling of 
the additive and premixtures: ‘The supple ­
mentation with monosodium glutamat e, in 
particular via water for drinking, should take 
into account all essential and conditionally 
essential amino acids in order to avoid im­
balances.’12 Sept ember 2032EN Offi cial Jour nal of the European Union 23.8.2022 L 218/23  For the quantification of monosodium 
glutamate in premixtures:
— Ion-exc hang e chromat ography coupled 
with post-column derivatisation and visi­
ble or fluorescence detection 
(IEC- VIS/FLD) or
— Ion-exc hang e chromat ography coupled 
with post-column derivatisation and 
photomet ric detec tion (IEC- VIS), Com ­
mission Regulation (EC) No 152/2009 
(Annex III, F)
For the quantification of monosodium 
glutamate in feedingstuffs:
— Ion exchange chromat ography coupled 
to post-column derivatisation and photo ­
metr ic detec tion (IEC- VIS), Commission 
Regulation (EC) No 152/2009 (Annex III, 
F)
(1)Details of the analytical methods are available at the following address of the Reference Laborato ry: https://joint-research-c entre.ec.europa.eu/eurl-fa-eurl-f eed-additives/eurl-fa-author isation/eurl-f a-evaluation- 
repor ts_enEN Offi cial Jour nal of the European Union L 218/24 23.8.2022  Identifi -
cation 
number 
of the 
additiveAdditiveComposition, chemical formula, descr iption, 
analytical method.Species or 
categ ory of 
animalMaxi -
mum 
ageMini -
mum 
conten tMaximum 
conten t
Other provisi onsEnd of period of 
author isationmg active  
substance/kg of 
complet e feed with a 
moisture content of 
12 %
Categor y: Sensor y additiv es. Functional group:
Flav our ing compounds
2b621ii Monosodium 
glutamateAdditive composition
Monosodium glutamate
Char acter isation of the active substance
Monosodium glutamate produced by 
Corynebacter ium glutamicum NITE BP-01681
Purity: ≥ 99 %
Chemical formula: C5H8NaNO 4·H2O
CAS number: 6106-04-3
Einecs number: 205-538-1
Analytical method (1)
For the identification of monosodium 
L-glutamate monohydrat e in the feed 
additive:
— Food Chemical Codex monographs: 
‘Monosodium L-glutamat e’
For the quantification of monosodium 
glutamate in the feed additive:
— Ion-exc hang e chromat ography coupled 
with post-column derivatisation and visi­
ble or fluorescence detection 
(IEC- VIS/FLD) or
— Ion-exc hang e chromat ography coupled 
with post-column derivatisation and 
photomet ric detection (IEC- VIS)All animal 
species- - - 1.The additive shall be incor porate d into the 
feed in the form of a premixture.
2.In the directions for use of the additive and 
premixture, the storage conditions and the 
stability to heat treatment shall be indicated.
3.On the label of the additive the following 
shall be indicate d: ‘Recommended maxi ­
mum cont ent of the active substance of 
com plete feed with a moisture cont ent of 
12 %: 25 mg/kg.’
4.The functional group, the identifi cation num ­
ber, the name and the added amount of the 
active substance shall be indicat ed on the label 
of the premixture, where the use level on the 
label of the premixture would result in exceed ­
ing the level of active substance in comp lete 
feed refer red to in point 3.
5.For users of the additive and premixtures, 
feed business operat ors shall establish opera ­
tional procedures and organisational mea ­
sures to address the poten tial risks by inhala ­
tion or dermal contact. Where those risks 
cannot be eliminated or reduced to a mini ­
mum by such procedures and measures, the 
additive and premixtures shall be used with 
appropr iate personal protect ive equipment, 
including breathing protection.12 Sept ember 2032EN Offi cial Jour nal of the European Union 23.8.2022 L 218/25  For the quantification of monosodium 
glutamate in premixtures:
— Ion-exc hang e chromat ography coupled 
with post-column derivatisation and visi­
ble or fluorescence detection 
(IEC- VIS/FLD) or
— Ion-exc hang e chromat ography coupled 
with post-column derivatisation and 
photomet ric detec tion (IEC- VIS), Com ­
mission Regulation (EC) No 152/2009 
(Annex III, F)
(1)Details of the analytical methods are available at the following address of the Reference Laborato ry: https://joint-research-c entre.ec.europa.eu/eurl-fa-eurl-f eed-additives/eurl-fa-author isation/eurl-f a-evaluation- 
repor ts_enEN Offi cial Jour nal of the European Union L 218/26 23.8.2022