COMMISSION IMPLEMENTING REGUL ATION (EU) 2022/320 
of 25 Febr uary 2022
concer ning the author isation of expressed mandar in essential oil as a feed additiv e for poultr y, pigs, 
ruminants, horses, rabbits and salmonids 
(Text with EEA relevance) 
THE EUR OPEAN COMMISSION,
Havi ng regar d to the Treaty on the Functioning of the European Union,
Havi ng rega rd to Regulation (EC) No 1831/2003 of the European Parliament and of the Council of 22 Sept ember 2003 on 
additives for use in animal nutr ition (1), and in particular Article 9(2) thereof,
Whereas:
(1) Regulation (EC) No 1831/2003 provid es for the author isation of additives for use in animal nutr ition and for the 
grounds and procedures for granting such an author isation. Article 10(2) of that Regulation provides for the 
re-evaluation of additives author ised pursuant to Council Directive 70/524/EEC (2).
(2) Mandar in essential oil was author ised without a time limit in accordance with Directive 70/524/EEC as a feed 
additive for all animal species. This additive was subsequently entered in the Register of feed additives as an existing 
product, in accordance with Article 10(1)(b) of Regulation (EC) No 1831/2003.
(3) In accordance with Article 10(2) of Regulation (EC) No 1831/2003 in conjunction with Article 7 thereof, an 
application was submitted for the re-evaluation of expressed mandar in essential oil for all animal species.
(4) The applicant request ed expressed mandar in essential oil to be author ised for use also in water for drinking. 
However , Regulation (EC) No 1831/2003 does not allow the author isation of flavouring compounds for use in 
water for drinking. Theref ore, the use of expressed mandar in essential oil in water for drinking should not be 
allowe d.
(5) The applicant requeste d the additive to be classifie d in the additive catego ry ‘sensor y additives ’ and in the functional 
group ‘flavouring compo unds ’. That application was accompan ied by the particulars and documents required under 
Article 7(3) of Regulation (EC) No 1831/2003.
(6) The European Food Safety Author ity (‘the Author ity’) concluded in its opinion of 5 May 2021 (3)that, under the 
proposed conditions of use, expressed mandar in essential oil does not have adverse effects on animal health, 
consumer health or the environment. However , no conclusions could be drawn for compan ion animals and 
ornamental fish not normally exposed to citrus by-products. The Author ity also concluded that expressed 
mandar in essential oil should be considered as a skin sensitiser and as an irritant to skin, eyes and the respirato ry 
tract. Theref ore, the Commission considers that appropr iate prot ective measures should be taken to prevent adverse 
effects on human health, in particular as regards the users of the additive.
(7) The Authority also concluded, that expressed mandar in essential oil is recognised to flavour food and its function in 
feed would be essentially the same as that in food, theref ore, no further demonstration of efficacy is considered 
necessar y. It also verified the repor t on the methods of analysis of the feed additive in feed submitte d by the 
Reference Laboratory set up by Regulation (EC) No 1831/2003.
(1)OJ L 268, 18.10.2003, p. 29.
(2)Council Directive 70/524/EEC of 23 November 1970 concer ning additives in feedingstuffs 
(OJ L 270, 14.12.1970, p. 1).
(3)EFSA Jour nal 2021;19(6):6625.EN Offi cial Jour nal of the European Union 28.2.2022 L 55/41  (8) The assessment of expressed mandar in essential oil shows that the conditions for author isation, as provided for in 
Article 5 of Regulation (EC) No 1831/2003, are satisfi ed. According ly, the use of the substance should be 
author ised as specif ied in the Annex to this Regulation.
(9) The fact that expressed mandar in essential oil is not author ised for use as a flavouri ng in wate r for drinking, does not 
preclude its use in compo und feed which is administered via water .
(10) Since safet y reasons do not require the immediate application of the modifications to the conditions of author isation 
of the substance concer ned, it is appropr iate to allow a transitional period for interest ed parties to prepare 
themselves to meet the new requirements resulting from the author isation.
(11) The measures provid ed for in this Regulation are in accordance with the opinion of the Standing Committ ee on 
Plants, Animals, Food and Feed,
HAS ADOPTED THIS REGUL ATION:
Article 1
Author isation
The substance specified in the Annex, belonging to the additive cate gory ‘sensor y additives’ and to the functional group 
‘flavouring compo unds ’, is author ised as feed additive in animal nutr ition, subject to the conditions laid down in that 
Annex.
Article 2
Transitional measures
1. The substance specified in the Annex and premixtures containing this substance, which are produced and labelled 
before 20 Sept ember 2022 in accordance with the rules applicable before 20 March 2022 may continue to be placed on 
the mark et and used until the existing stoc ks are exhaust ed.
2. Com pound feed and feed mate rials containing the substance as specifi ed in the Annex whic h are produced and 
labelled before 20 March 2023 in accordance with the rules applicable before 20 March 2022 may continue to be placed 
on the mark et and used until the existing stoc ks are exhaust ed if they are intended for food-producing animals.
Article 3
Entr y into force
This Regulation shall enter into force on the twentieth day followi ng that of its publication in the Official Journal of the 
European Union.
This Regulation shall be binding in its entirety and directly applicable in all Member States.
Done at Brussels, 25 Febr uary 2022 .
For the Commission
The President
Ursula VON DER LEYENEN Offi cial Jour nal of the European Union L 55/42 28.2.2022  ANNEX 
Identifica -
tion 
number of 
the additiveName 
of the 
holder 
of 
autho -
risa-
tionAdditiveCompo sition, chem ical formula, 
descr iption, analytical method.Species or category 
of animalMaximum 
ageMinimum 
conten tMaximum 
conten t
Other provisi onsEnd of period of 
author isationmg active substance/kg 
of complet e feed with a 
moisture conten t of 
12 %
Categor y: Sensor y additiv es. Functional group: Flavour ing compounds
2b142-eo - Expressed 
mandar in 
essential oilAdditive composition
Mandar in essential oil obtained 
from fruit peel of Citrus reticulata 
Blanco.
Liquid form
Char acter isation of the active 
substance
Expressed mandar in essential oil 
obtained by cold expression from 
fruit peel of Citrus reticulata 
Blanco as defined by the Council 
of Europe (1).
d-Limonene: 65-80 %
γ-Terpinene: 13-22 %
α-Pinene (pin-2(3)-ene):
1-3,5 %
Myrcene:1-2 %
β-Pinene (pin-2(10)-ene):
1-2 %.
Methyl N-meth yl anthranilate: 
0,15-0,7 %
Perillaldeh yde: ≤ 0,063 %
CAS number: 8008-31-9
FEMA number: 2657
CoE number: 142Poultr y
Rabbits
Salmonids- - 15 1.The additive shall be incor porate d into the 
feed in the form of a premixture.
2.In the directions for use of the additive and 
premixtures, the storag e conditions and sta­
bility to heat treatment shall be indicated .
3.Mixture of expressed mandar in essential oil 
with other botanical additives is permitted 
provided that the amounts of perillaldeh yde 
in feed mater ials and compo und feed are low­
er than the one resulting from the use of a 
sing le additive at the maximum or recom ­
mended level for the species or animal cate ­
gory.
4.For users of the additive and premixtures, 
feed business operat ors shall establish opera ­
tional procedures and organisational mea ­
sures to address potential risks by inhalation, 
dermal contact or eyes contact. Where those 
risks cannot be eliminated or reduced to a 
minimum by such procedures and measures, 
the additive and premixtures shall be used 
with personal protect ive equipment, includ ­
ing skin, eye and breathing prot ection.20 Marc h 2032
Pigs - - 33
Ruminants - - 30
Horses - - 40EN Offi cial Jour nal of the European Union 28.2.2022 L 55/43  Analytical method (2)
For the quantification of 
phytochemical marker 
d-limonene in the feed additive or 
in mixture of flavouring 
compo unds:
— Gas chromat ography 
coupled with flame ionisa ­
tion detection (GC-FID) 
(based on ISO 3528)
(1)Natural sources of flavourings – Repor t No 2 (2007).
(2)Details of the analytical methods are avail able at the follo wing address of the Reference Laboratory : https://ec.europa.eu/jrc/en/eurl/feed-additives/evaluation-repor tsEN Offi cial Jour nal of the European Union L 55/44 28.2.2022