COMMISSION IMPLEMENTING REGUL ATION (EU) 2020/1373 
of 1 Oct ober 2020 
concer ning the author isation of zinc chelate of lysine and glutamic acid as a feed additiv e for all 
animal species 
(Text with EEA relevance) 
THE EUR OPEAN COMMISSION, 
Havi ng regar d to the Treaty on the Functioning of the European Union, 
Havi ng rega rd to Regulation (EC) No 1831/2003 of the European Parliament and of the Council of 22 Sept ember 2003 on 
additives for use in animal nutr ition (1), and in particular Article 9(2) thereof, 
Whereas: 
(1) Regulation (EC) No 1831/2003 provid es for the author isation of additives for use in animal nutr ition and for the 
grounds and procedures for granting such author isation. 
(2) In accordance with Article 7 of Regulation (EC) No 1831/2003 an application was submitted for the author isation 
of zinc chelate of lysine and glutamic acid. That application was accompan ied by the particulars and documents 
required under Article 7(3) of that Regulation. 
(3) That application concer ns the author isation of zinc chelate of lysine and glutamic acid as a feed additive for all 
animal species to be classified in the additive cate gory ‘nutr itional additives ’. 
(4) The European Food Safet y Authority (‘the Author ity’) concluded in its opinion of 2 July 2019 (2) that, under the 
proposed conditions of use, zinc chelate of lysine and glutamic acid does not have an adverse effect on animal 
health and consumer health. It also concluded that the additive is considered as a potential skin and respirato ry 
sensitizer and stated a risk for the users of the additive upon inhalation. Theref ore, appropr iate protective measures 
should be take n to prevent adverse effects on human health, in particular as regar ds the users of the additive. The 
Authority also concluded that that additive does not pose an additional risk for the environment compare d to other 
compo unds of zinc and that it is an efficacious source of zinc for all animal species. The Auth ority does not consider 
that there is a need for specific requirements of post-market monitoring. It also verified the repor t on the method of 
analysis of the feed additive in feed submitted by the Reference Laborator y set up by Regulation (EC) No 1831/2003. 
(5) The assessment of that additive shows that the conditions for author isation, as provid ed for in Article 5 of 
Regulation (EC) No 1831/2003, are, subject to the relevant prot ective measures for the users of the additive, 
satisfied. According ly, the use of that additive should be author ised as specif ied in the Annex to this Regulation. 
(6) The measures provid ed for in this Regulation are in accordance with the opinion of the Standing Committ ee on 
Plants, Animals, Food and Feed, 
HAS ADOPTED THIS REGUL ATION: 
Article 1 
The substance specifi ed in the Annex, belonging to the additive categor y ‘nutr itional additives ’ and to the functional group 
‘compounds of trace elements’, is author ised as an additive in animal nutr ition subject to the conditions laid down in that 
Annex. 
(1)OJ L 268, 18.10.2003, p. 29. 
(2)EFSA Jour nal 2019;17(7):5782. EN Offi cial Jour nal of the European Union 2.10.2020                                                                                                                                         L 319/15   Article 2 
This Regulation shall enter into force on the twentieth day followi ng that of its publication in the Official Journal of the 
European Union. 
This Regulation shall be binding in its entirety and directly applicable in all Member States. 
Done at Brussels, 1 October 2020.  
For the Commission 
The President 
Ursula VON DER LEYEN     EN Offi cial Jour nal of the European Union L 319/16                                                                                                                                         2.10.2020   ANNEX 
Identif i-
cation 
number 
of the 
additiv e Name of 
the holder 
of 
author isa-
tion Additiv e Composition, chemical form ula, descr iption, 
analytical method Species or 
categor y of 
animal Maxi-
mum 
age Mini-
mum 
content Maximum 
content 
Other provisions End of period 
of 
author isation Content of element (Zn) in 
mg/kg of complete feed 
with a moistu re content of 
12 % 
Categor y of nutr itional additiv es. Functional group: compounds of trace elements 
3b615 - Zinc chelate of 
lysine and gluta­
mic acid Additiv e composition: 
Mixture of chelates of zinc with lysine and che­
lates of zinc with glutamic acid in a ratio of 1:1 
as a powder with 
a zinc cont ent between 17 and 19 %, 
a lysine cont ent between 19 and 21 %, 
a glutamic acid content between 21 and 23 % 
and a maximum of 3 % moisture All animal  
species - - Dogs and cats: 
200 (total) Sal­
monids and milk 
replacers for 
calves: 180 (total) 
Piglets, sows, rab­
bits and all fish 
other than salmo­
nids: 150 (total) 
Other species and 
cate gories: 120 
(total) 1.The additive shall be in­
corporat ed into feed in 
the form of a premixture. 
2. Zinc chelate of lysine and 
glutamic acid may be 
placed on the mark et 
and used as an additive 
consisting of a prepara­
tion. 
3. For users of the additive 
and premixtures, feed 
business operators shall 
establish operational pro­
cedures and appropr iate 
organisational measures 
to address the potential 
risks by inhalation, der­
mal or eyes contact. 
Where risks cannot be re­
duced to an acceptable le­
vel by these procedures 
and measures, the addi­
tive and premixtures shall 
be used with appropr iate 
personal prote ctive 
equipment, including 
breathing protection. 22.10.2030. 
Character isation of the activ e subs tances: 
Chemical formulas: 
Zinc-2,6-diaminohexanoic acid, chlor ide and 
hydrogen sulfate salt: 
C6H19ClN 2O8SZn 
Zinc-2-aminopentanedioic acid, sodium and 
hydrogen sulfate salt: 
C5H8NNaO 8SZn 
Analytical methods *: 
For the quantifi cation of the lysine and gluta­
mic acid content in the feed additive: 
— ion exchange chromat ography coupled 
with post-column derivatisation and 
phot ometr ic detec tion (IEC- VIS) 
For the quantification of total zinc in the feed 
additive: 
—inductively coupled plasma atom ic emis­
sion spectrometr y, ICP-AES (EN 15 621) or 
— atomic absor ption spectrometr y, AAS  
(ISO 6869) EN Offi cial Jour nal of the European Union 2.10.2020                                                                                                                                         L 319/17   Identif i-
cation 
number 
of the 
additiv e Name of 
the holder 
of 
author isa-
tion Additiv e Composition, chemical form ula, descr iption, 
analytical method Species or 
categor y of 
animal Maxi-
mum 
age Mini-
mum 
content Maximum 
content 
Other provisions End of period 
of 
author isation Content of element (Zn) in 
mg/kg of complete feed 
with a moistu re content of 
12 % 
For proving the chelated structure of the feed 
additive: 
— mid-infrared (IR) spectrometr y together 
with the determination of the content of 
the trace element and lysine and glutamic 
acid in the feed additive 
For the quantification of total zinc in premix­
tures: 
—inductively coupled plasma atom ic emis­
sion spectrometr y, ICP-AES (EN 15 510 
or EN 15 621) or 
— atomic absor ption spectrometr y, AAS (ISO 
6869) or 
—inductively coupled plasma mass spectro­
metr y, ICP-MS (EN 17 053) 
For the quantifi cation of total zinc in feed ma­
terials and compo und feed: 
—inductively coupled plasma atom ic emis­
sion spectrometr y, ICP-AES (EN 15 510 
or EN 15 621) or 
— atomic absor ption spectrometr y, AAS 
(Commission Regulation (EC) 
No 152/2009 (Annex IV-C) or ISO 6869) 
or 
—inductively coupled plasma mass spectro­
metr y, ICP-MS (EN 17 053) 
*Details of the analytical methods are avail able at the follo wing address of the Reference Laboratory : https://ec.europa.eu/jrc/en/eurl/feed-additives/evaluation-repor ts   EN Offi cial Jour nal of the European Union L 319/18                                                                                                                                         2.10.2020