DRAFT UGANDA STANDARD  
  DUS 466  
Second  Edition  
2020 -mm-dd 
 
 
 Reference number  
DUS 466: 2020  
© UNBS 2020   
  
Manual Tooth brush  — Specification  
 DUS 466: 2020  
ii © UNBS2020 – All rights reserved  
 Compliance with this standard does not, of itself confer immunity from legal obligations  
A Uganda Standard does not purport to include all necessary provisions of a contract. Users are 
responsible for its correct applic ation  
© UNBS  2020  
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilised in any 
form or by any means, electronic or mechanical, including photocopying and microfilm, without prior written 
permission from UNBS.  
Requests for permission to reproduce this document should be addressed to  
The Executive Director  
Uganda National Bureau of Standards  
P.O. Box 6329  
Kampala  
Uganda  
Tel: +256 41 4 333 2 50/1/2/3  
Fax: +256 41 4 286 123  
E-mail: info@unbs.go.ug  
Web: www.unbs.go.ug  DUS 466; 2020  
© UNBS 2020  – All rights reserved  iii 
 Contents  Page 
Foreword  ................................ ................................ ................................ ................................ ............................  iv 
1 Scope  ................................ ................................ ................................ ................................ ......................  1 
2 Normative references  ................................ ................................ ................................ ............................  1 
3 Terms and definitions  ................................ ................................ ................................ ...........................  1 
4 Sizes ................................ ................................ ................................ ................................ ........................  3 
5 Classification  ................................ ................................ ................................ ................................ .........  3 
6 Requirements  ................................ ................................ ................................ ................................ .........  3 
6.1 General requirements  ................................ ................................ ................................ ...........................  3 
6.1.1  Cleaning Element  ................................ ................................ ................................ ................................ .. 3 
6.1.2  Shaft  ................................ ................................ ................................ ................................ ........................  4 
6.1.3  Anchor Wire  ................................ ................................ ................................ ................................ ...........  4 
6.1.4  Tufting ................................ ................................ ................................ ................................ .....................  4 
6.1.5  Head  ................................ ................................ ................................ ................................ ........................  5 
6.2 Specific requirements  ................................ ................................ ................................ ...........................  5 
6.2.1  End rounding  ................................ ................................ ................................ ................................ .........  5 
6.2.2  Tuft anchorage  ................................ ................................ ................................ ................................ ....... 5 
6.2.3  Handle impact strength ................................ ................................ ................................ .........................  5 
6.2.4  Chemical challenge  ................................ ................................ ................................ ...............................  5 
6.2.5  Fatigue Resistance  ................................ ................................ ................................ ................................  5 
7 Labelling  ................................ ................................ ................................ ................................ .................  5 
8 Packaging  ................................ ................................ ................................ ................................ ...............  6 
9 Sampling  ................................ ................................ ................................ ................................ ................  6 
Annex  A (normative)  Determination of end rounding  ................................ ................................ ...................  7 
A.1 Test specimen  ................................ ................................ ................................ ................................ ........  7 
A.2 Apparatus  ................................ ................................ ................................ ................................ ...............  7 
A.3 Procedure  ................................ ................................ ................................ ................................ ...............  7 
A.4 Calculation  ................................ ................................ ................................ ................................ .............  7 
Annex  B (Normative)  Tuft anchorage test  ................................ ................................ ................................ ...... 9 
B.1 Apparatus  ................................ ................................ ................................ ................................ ...............  9 
B.2 Procedure  ................................ ................................ ................................ ................................ ...............  9 
Annex  C (normative)  Handle impact test  ................................ ................................ ................................ ...... 11 
C.1 Apparatus  ................................ ................................ ................................ ................................ .............  11 
C.2 Procedure  ................................ ................................ ................................ ................................ .............  11 
C.3 Test report  ................................ ................................ ................................ ................................ ............  13 
Annex  D (normative)  Fatigue resistance test  ................................ ................................ ...............................  14 
D.1 Apparatus  ................................ ................................ ................................ ................................ ...................  14 
D.2 Procedure  ................................ ................................ ................................ ................................ .............  14 
Annex  E (normative)  Resistance to chemical challenge  ................................ ................................ .............  15 
E.1 Apparatus and chemicals  ................................ ................................ ................................ ...................  15 
E.2  Procedure  ................................ ................................ ................................ ................................ .............  15 
Annex  F (Normative)  Samp ling and criteria for conformity  ................................ ................................ ........  16 
F.1 Scale of sampling  ................................ ................................ ................................ ................................  16 
Bibliography  ................................ ................................ ................................ ................................ ......................  17 
 DUS 466: 2020  
iv © UNBS2020 – All rights reserved  
 Foreword  
Uganda Nationa l Bureau of Standards (UNBS) is a parastatal under the Ministry of Trade , Industry and 
Cooperatives established under Cap 327 , of the Laws of Uganda , as amended .  UNBS is mandated to co -
ordinate the elaboration of standards and is  
(a) a member of Internationa l Organisation for Standardisation (ISO) and  
(b) a contact point for the WHO/FAO Codex Alimentarius Commission on Food Standards, and  
(c) the National Enquiry Point on TBT  Agreement  of the World Trade Organisation (WTO).  
The work of preparing Uganda Standards is  carried out through Technical Committees. A Technical 
Committee is established to deliberate on standards in a given field or area and consists of key stakeholders 
including government, academia, consumer groups, private sector and other interested partie s.  
Draft Uganda Standards adopted by the Technical Committee are widely circulated to stakeholders and the 
general public for comments. The committee reviews the comments before recommending the draft standards 
for approval and declaration as Uganda Stand ards by the National Standards Council.  
The committee responsible for this document is Technical Committee UNBS /TC5 , Chemicals and 
Environment , Subcommittee SC3 , Plastics and Related products . 
This second  edition cancels and replaces the first edition ( US 466:2006 ), which has been technically revised.  
 
 DRAFT UGANDA STANDARD  DUS 466: 2020  
 
© UNBS 2020  – All rights reserved  1 
 Manual t oothbrush — Specification  
1 Scope   
This Daft Uganda standard specifies the requirements, sampling and methods of test for manual toothbrushes 
manufactured for oral hygiene.  
2 Normative references   
The following referenced documents referred to in the text in such a way that some or all of their content 
constitutes requirements of this document. For dated references, only the edition cited applies. For undated 
references, the latest edition of the re ferenced document (including any amendments) applies.  
ISO 3696, Water for analytical laboratory use — Specification and test methods  
3 Terms and definitions   
For the purposes of this document, the following terms and definitions apply.  
ISO and IEC maintai n terminological databases for use in standardization at the following addresses:  
— ISO Online browsing platform: available at http://www.iso.org/obp  
3.1  
toothbrush  
an implement comprising of the handle  and head, designed to help in oral hy giene  
3.2 
hand le 
body of the  tooth brush excluding  the head  
3.3 
lot 
quantity of toothbrushes of the same size, grade, materials and have the same batch identification, from one 
manufacturer, submitted at any one time for inspection and testing  
3.4 
head  
stock and the fila ments of the toothbrush  
3.5 
shaft  
complete toothbrush except the tufts 
3.6 
stock  
part supporting the tufts   
2 © UNBS  2020  – All rights reserved  
 3.7 
tuft 
cluster of aggregate of mono filaments /bristles  fixed in one hole in the stock  
3.8 
monofilaments  
single strand of material in a  tuft 
3.9 
cleaning element  
protrusions fixed on the brush head, which is designed to impart the cleaning of the tooth surface, 
interproximal spaces and near gum line. It consist s of filaments;  tufts made of filaments or specially designed 
elements (for example Lamella)  of synthetic materials,  see fig 1  
3.10 
end rounding  
shape of th e tip of a monofilament  
3.11 
pull strength  
force require d to pull out a tuft  from the head of  a toothbrush  
3.12 
pendulum length (Lp)  
distance, expressed in metres, between the axis of rotatio n of the pendulum a nd the centre of percussion 
(3.13 ), equal to an equivalent theoretical pendulum mass concentrated at the point which gives the same  
period of oscillation , as the actual pendulum  
3.13 
centre of percussion  
point on pendulum at which a perp endicular impact in the plane of swing does not cause reaction forces at the 
axis of rotation of the pendulum  
3.14 
period of oscillation of the pendulum (Tp)  
period, expressed in seconds, of a single complete oscillation (to and fro) of the pendulum, oscil lating at 
angles of oscillation of less than 5° to each side of the vertical  
 
Figure1 — Brush handle and head  
Key 
A shaft , excluding L  
B stock  
L cleaning element / filaments  
B and L  head  
DUS  466: 2020  
© UNBS  2020 – All rights reserved  3 
 4 Sizes  
Toothbrushes shall be of the foll owing sizes  depending on the ir length .  
Table 1 — Length for toothbrush es 
Size Minimum length of Toothbrush es 
mm 
Adult  (Above 18 years)  150 
Junior  (3 years to 17 years)  120 
Baby  (2 years and below ) 90 
 
5 Classification  
A toothbrush shall be  classified under the  following types depending on the gauge of the filaments  
a) Super -soft; 
b) Soft; 
c) Medium;  
d) Hard  and  
e) Dual/multi texture . 
6 Requirements  
6.1 General requirements  
The toothbrush shall be intact and fr ee from  visible contamination, sharp and rough surfaces w hen examined  
6.1.1  Cleaning Elemen t 
6.1.1.1  It shall consist of nylon filaments — 66; 610; 612, polyester filaments, poly butylene terephthalate 
filaments, thermoplastic elastomer, polyethylene filaments, polypropylene filaments, or any comb inations of 
above mat erials  
6.1.1.2  It shall be free from any disagreeable odour, taste and toxic elements  
6.1.1.3  The colours  used on the  cleaning element shall be non -toxic  
6.1.1.4 The filaments shall be in the form of monof ilament, co -extruded filaments, filled filaments,  or 
structured filaments.  
6.1.1.5 The cross -section of the filaments shall be of circular, square, hexagonal, diamond, rectangular, 
or any other suitable shape.  
6.1.1.6 The diameter of filaments used a s filling material in the cleaning element of the tooth brush , shall 
be as given in Table 2 . Tolerance limit for individual filam ent shall be  ±0.02 mm of claimed diameter  and 
average of fifty filaments shall fall in the range specified in Table  2  
4 © UNBS  2020  – All rights reserved  
 Table 2  — Diameter  and minimum number of monofilaments  
SN Size Type Diameter of Filament s 
mm Minimum number 
of monofilament s 
1 Adult  Super soft  0.12 ≤ 0.15 300 
2 Adult  Soft 0.15 ≤ 0.20 300 
3 Adult  Medium  0.20 ≤ 0.25  300 
4 Adult  Hard  0.25 ≤ 0.35 300 
5 Junior  Soft 0.15 ─ 0.20 250 
6 Baby  Soft 0.12 ─ 0.18  250 
 
6.1.2 Shaft  
6.1.2.1 The shaft shall be manufact ured from the following plastic materials and shall  be free f rom any 
odour  
a) Polystyrene moulding powder ; 
b) Polypropylene moulding powder (Homo  polymer, co -polymer) ; 
c) Cellulose ac etate propionate moulding powder;  
d) Styrene  acrylonitrile butadiene styrene  (SAN) ; 
e) Polyethylene terephthalate ; 
f) Polycarbonate ; 
g) Acrylonitrile butadiene styrene  (ABS) ; 
h) Methacrylate butadiene styrene  (MBS) ; 
i) Poly methyl methacrylate  and 
j) Thermoplastic elastomer  (TPE) . 
The manufacturer may ch oose to desig n the shaft from one or more polymers from above list  
6.1.2.2 For ease of handgrip during use,  the shaft may  have extra indentations or appendages  
6.1.3 Anchor Wire  
Anchor wire if  used fo r toothbrush shall be manufactured by tufting/staple technology. It s hall consist of 
aluminium, brass, nickel, silver, or any other suitable non -corrosive materials  
6.1.4 Tufting  
6.1.4.1 Cleaning element shall  be fixed to brush head using a suitable technology such as staple 
technology using an anchor wire, welding technolo gy, fusion technology, in mould welding technology etc.  
6.1.4.2 In case of toothbrushes in which anchor wire is used for fixing  each cleaning element which is in 
the form of a tuft , it shall be individually fixed in a suitable sized tuft hole on the brush head and sunk to the 
depth up to start of bottom cup of the hole.  DUS  466: 2020  
© UNBS  2020 – All rights reserved  5 
 6.1.4.3 The maximum protrusion of the cleaning elements from surface of head shall be 13 mm. and the 
minimum shall be 7mm  
6.1.4.4 The tops of tufts may be trimmed to any profile to suit indi vidual design but shall be in a manner, 
which does not injure the gums while brushing.  
6.1.4.5  The maximum width of head of the toothbrush shall not be more than 13 mm for recta ngular 
head, i n case of adult; 12 mm in case of junior and 9 mm in case of baby  size. 
6.1.5 Head  
The brush  head  shall not have any protruding parts that may injure the user.  
6.2 Specific  requirements  
6.2.1 End rounding  
The heads of monofilaments shall be polished to give them round shape. The end rounding shall not be less 
than 50 pe rcent when determined according to the method given in Annex A  
6.2.2 Tuft anchorage  
The force required for pulling out an individual tu ft shall not be less than 10 N  when tested according to the 
method given in Annex B.  
6.2.3  Handle impact strength  
When tested in accordance with  the test method given in Annex C , the handle should not fracture. If the 
handle does fracture, however, the minimum absorbe d energy at fracture shall be 0. 8 J 
6.2.4  Chemical challenge  
The toothbrush shall comply with 6.2.5  after bei ng subj ected to a chemical challenge as  given in Annex E  
6.2.5  Fatigue Resistance  
The toothbrush shall complete 75 000 cycles without breaking when tested in accordance to test method 
given in Annex D   
NOTE: A cycle is one application of force followed by removal of the force  
7 Labelling  
The container for each toothbrush  shall be legibly and indelibly marked with the following  information :  
a) manufacturer’s name and physical address ; 
b) type of brush e.g. soft, hard;  
c) country of origin ; 
d) size of brush e.g. Adult, Junior, Baby ; 
e) batch number ;  
f) composition of monofilaments e.g. ABS, MBS,OR ABS and MBS and   
6 © UNBS  2020  – All rights reserved  
 g) type of polymer  
8 Packaging  
The packaging shall be such that it will neither contaminate nor permit contamination of the toothbrush.  The 
head of the toothbrush sha ll be visible w hen it is in  the container  and each toothbrush shall be individually 
packed.  
9 Sampling  
Sampling shall be done in accordance with  Annex F . DUS  466: 2020  
© UNBS  2020 – All rights reserved  7 
 Annex  A 
(normative)  
 
Determination of end rounding  
A.1 Test specimen  
Three  of the toothbrushes in the samp le taken in accordance with Annex F  
A.2 Apparatus  
Simple or compound microscope (advisable) with adequate magnification to observe the end rounding o f 
filament tips  
A.3 Procedure  
A.3.1  Remove the filaments from three  tufts in each of the toothbrus hes u nder test. By means of a 
microscope,  examine the free end of each filament. Record the appearance of each filament free end as 
acceptable (including ideal) or not acceptable, using the example s depicted in Fig.A1  as criteria for 
judgement.  
Retain the tooth brush es for the test described in Annex C  
A.3.2  Count the number of tips of monofil aments in each selected tufts; E1, E2, E3  
where  
E1 Number of ti ps of monofilament in first tuft  
E2  Number of tips  of monofilament in second tuft  and 
E3 Number of ti ps of monofilament in third tuft . 
A.3.3 Strand Count = E1 or E 2 or E 3 
A.3.4 Observe the end rounding of the tips of monofilament from each selected tufts under microscope and 
count the number of tips with acceptable end rounding. The tips of monofilament with an y sort of sharp 
angles/corners or edges shall be unacceptable and those without any such sharp angles/corners or edges 
shall be acceptable, note down the findings  OK 1, OK 2 and OK 3 
where: 
OK 1  Number of tips of acceptable monofilament in first tuff ; 
OK 2  Number of tips of acceptable monofilament in second tuff, and  
OK 3  Number of tips of acceptable monofilament in third tuff . 
A.4 Calculation  
A.4.1  End rounding, percent   
8 © UNBS  2020  – All rights reserved  
 
 
A.4.2. Repeat the same procedure for two more toothbrushes from a lot  
A.4.3 Take a verage  of end rounding of three toothbrushes.  
 
 
Figure A1 — Guidelines  for acceptable  and unacceptable  end rounding  
DUS  466: 2020  
© UNBS  2020 – All rights reserved  9 
 Annex  B 
(Normative ) 
 
Tuft anchorage  test 
B.1 Apparatus  
B.1.1  A simp le instrument as shown in Fig ure B1  can be used for testing the pull strength. This unit is 
suitable for mounting on a wall. It consists of dial force gauge/weighing scale operating on s pring(B) mounted 
on wooden ply (B ). A tubular tuft -holder (C) is hung o n the hook of dial gauge. A clamp for holding toothbrush 
(E) is provided which is movable downward and upward with a screw (G).  
NOTE  — Manufacturer may use sophisticated electronic instrument available in marke t to determine the tuft anchorage 
strength  
B.1.2 A water bath containing distilled water maintained at 60 ± 2˚C. 
B.1.3  A water bath containing distilled water maintained at 4 ± 1˚C. 
B.2 Procedure  
B.2.1  Immerse the head of the toothbrush unde r test in the water bath at 60 ± 2˚C for a period of 2 min  After 
2 min remove the toothbrush f rom the water and p lace it in the water bath at 4 ± 1 ˚C for a further 2 min  
B.2.2 Immediately completing B.2.1 fix the toothbrush head with tufts in upward  direction in the toothbrush 
holder with the help of screw (F).  
B.2.3 Insert all filaments of one tuft in  the hole provided at the  bottom of tubular tuft -holder (C). Care should 
be taken not to allow filaments  from adjacent tuft to enter in to the hole. Fix the tuft firmly with  the help of screw 
(D). 
B.2.4 Adjust the pointer on dial to zero by adjustment of screw (G)  
B.2.5 Move down t he toothbrush holder slowly with screw (G) watching the pointer on dial carefully. Note 
down the reading on dial at which the tuft comes out of the hole. This is the pull strength of the tuft.   
10 © UNBS  2020  – All rights reserved  
 
 
FigureB1 — Instrument for determination of pull strength  
 
 DUS  466: 2020  
© UNBS  2020 – All rights reserved  11 
 Annex C 
(normative)  
 
Handle impact test  
C.1 Apparatus  
C.1.1 Clamping unit, to hold the toothbrush handle  (see Figure C1). The clamping unit consists of the main 
block (key item 4) and the holding blocks (key ite ms 6 and 7) . The holding blocks can be moved by  screws. 
The radius of the internal edges of the main block and the holding blocks should be (4˖0 ± 0˖1) mm.  
C.1.2 Impact tester , with the striker  for the pendulum  
C.1.3 Striker for pendulum  made of hardened steel, with a cylindrical surface hav ing a radius of curvature 
of (0˖80 ± 0˖20) mm, with its axis horizontal and perpendicular to the plane of swing of the pendulum.  
C.2 Procedure  
Hold the toothbrush han dle using the clamping unit ( C.1.1) with a clamping torque of (0 ˖70 ± 0˖03) N m. 
Ensure that the tuft -hole plane (key item 1) is perpendicular to the bottom plane of the clamping unit (key item  
5). The metal plate(s) can be used as the spacer to adjust the angle. Set the clamping unit on the impact 
tester so that the striker edge can hit the toothbrush handle at the centre of the tuft -hole area (key item 8) from 
the opposite side of the tuft -hole surface (key item 2).  
The length, L1, between the top plane of the clamping unit (key item 3) and the centre of the tuft -hole area 
(key item 8) is 55 mm. Any curvature of the corners of the clamping unit shall not be taken into account for L1. 
NOTE  1 The impact velocity of the striker is dependent on the height of the striker at the beginning of the test, or the 
vertical distance of fall of the pendulum striking edge. This height is a function of the length of the pendulum arm and the 
angle of the arm at  the beginning of the test. The velocity of the striker edge at impact can be calculated by the following 
equation:  
 
where  
V is the velocity of the striker at the moment of impact, in metres per second  
g is the local gravitational acceleration, in metres per second squared  
h is the vertical distance of fall of the pendulum striking edge, in metres  
NOTE 2  ASTM D256 -06 sets the vertical distance of the pendulum striking edge to be (610 ± 2˖0) mm, which will 
produce a velocity of the striking edge at the mome nt of impact of approximately 3 ˖5 m/s.  
Ensure that the distance between the line of contact of the pendulum striking edge and the centre of 
percussion  of the pendulum is less than 2˖ 54 mm  
NOTE 3  ISO 13802 states that the impact length shall be within 1 % of the pendulum length. Since the pendulum 
length may vary with machines, the ASTM D256 -06 value of ±2,54 mm was chosen to be consistent across laboratories 
instead of the 1 % value specified in ISO 13802.   
12 © UNBS  2020  – All rights reserved  
 Determine the pendulum length, Lp (3˖12 ), in metre s, from the period of oscillation of the pendulum , Tp in 
seconds , using the following equation:  
 
where  
g Is the local gravitational acceleration , in metres per second squared and  
4π2 Equals 39 ˖48. 
Apply an energy ( initial potential energy) of (2˖ 75 ± 0˖10) J 
The test result is divided into fractured (F) or not fractured (NF). When the toothbrush is fractured, measure 
the angle of the pendulum and calculate the absorbed energy, Ea from the following equation:  
 
where  
W is the striker weight, in  newtons;  
R is the distance between the axis of rotation and the centre of gravity, in metres;   
ὰ is the angle at the test  starting position, in degrees;  
β  is the angle after breakage of the specimen, in degrees.  
 
Figure C1 — Clamping unit to hold the toothbrus  DUS  466: 2020  
© UNBS  2020 – All rights reserved  13 
 Key  
1 Tuft-hole plane  
2 Hitting point for striker edge  
3  Top plane of the clamping u nit  
4 Main block  
5 Bottom plane of the clamping unit  
6 Lower holding block  
7 Upper holding block  
8 Centre of tuft -hole area  
 NOTE  The open arrow in the figure shows the striker direction.  
C.3 Test report  
The test report shall include the following inf ormation:  
a) The number of this Standard, i.e. US 466:2020;  
b) An identification of the toothbrush;  
c The results and  
d) Any unusual features noted during the test  
  
14 © UNBS  2020  – All rights reserved  
 Annex  D 
(normative)  
 
Fatigue resistance test  
D.1 Apparatus  
D.1.1 Block for holding the toot hbrush body stationary, having a ridge for supporting the toothbrush at (55 ± 
1) mm from the centre of the brush head and a gripping unit for locking the handle of the toothbrush in place.  
D.1.2 Apparatus for applying a (4 ˖0 ± 0 ˖1) N force to the brush hea d and then fully relieving the force.  
D.1.3 Apparatus for counting the number of cycles completed.  
D.1.4 Apparatus for stopping the application of force when either of the following conditions has occurred:  
a) Completion of the required number of cycles;  
 b) Handle breakage.  
D.2 Procedure  
Cut the filaments, and any other attachments, flush to the brush head. Protect the brush head by covering the 
head with adhesi ve tape (thickness: less than 0 ˖2 mm). Place the toothbrush against the block with the tuft -
hole plane perpendicularly facing the applied force. Lock the toothbrush in place, ensuring that the ridge is at 
55 mm ± 1 mm from the centre of the brush head.  
NOTE  If the test specimen cannot b e locked in place due to its configuration, embed the specimen in epoxy resin or dental 
stone , ensuring that the surface level of epoxy resin or dental stone is at 55  mm ± 1 mm from the centre of the brush head.  
Apply a 4 .0 N ± 0.1 N force to the centre of  the brush head perpendicularly to the tuft -hole plane with minimal 
impact and then fully relieve the force. Repeat a maximum of 75 000 cycles at 50 cycles ± 10) cycles/min or 
until the handle breaks. Record the breakage if it is induced at less than 75 00 0 cycles.  DUS  466: 2020  
© UNBS  2020 – All rights reserved  15 
 Annex  E 
(normative)  
 
Resistance to chemical challenge  
E.1 Apparatus and chemicals  
E.1.1 Apparatus for mixing the chemical -challenge solution, e.g. stirring bar or mixer.  
E.1.2 Container, that can be sealed and is chemically inert, e.g. a glass bo ttle. 
E.1.3 Chemicals, as listed in Table E1  
Table E1 — Components of chemical challenge solution  
Chemical  Minimum purity  
% 
Ethanol  98˖5 
L-carvone  98˖0 
L-menthol  98.0 
Sodium lauryl sulphate  95.0 
Glycerine  98.0 
Water  ISO 3696 , Grade  
 
E.2  Procedure  
E.2.1  Add 1 ˖5 g of L -carvone, 1 ˖5 g of L -menthol and 15 g of sodium lauryl sulphate  to 100 g of ethanol in 
the container ( E.1.2) and sti r well  using the mixing apparatus ( E.1.1). Add 250 ml of Grade 3 water in 
accordance with ISO 3696 and stir well to obtain a clear solution. Add 200 g of glycerine and 432 ml of Grade 
3 water in accordance with ISO 3696 and stir to obtain a clear solution.  
E.2.2  Place the brush head and at least 80 % of a total length of the toothbrush in the chemical challenge 
solution. After 24 h without agitation, remove the toothbrush, rinse with Grade 3 water in accordance ISO 
3696 and shake off excess water. Perform t he test  as required in Annex D  (fatigue resistance test)   
16 © UNBS  2020  – All rights reserved  
 Annex  F 
(Normative)  
 
Sampling and criteria for conformity  
F.1 Scale  of sampling  
F.1.1 lot — In any consignment, all the brushes of the same size and quality sha ll be divided into groups 
and each such group shall constitute a lot. Care shall be taken to ensure that brushes included in a lot do not 
differ in construction as far as possible.  
F1.2 the conformity of the brushes in a lot to the requirements of this specification shall be ascertained for 
each lot separately. The number of brushes to be selected for this purpose sha ll be in accordance with Table 
F1 
Table. F1 — Scale o f sampling toothbrushes  
S/N Number of brushes in the lot  
(N) Number of brushes  to be selected  
(n) 
I.  Up to 50 2 
II.  51 to 150 3 
III.  151 to 200 4 
IV.  201 to 300 5 
V.  301 to 500 6 
vi 501 and above  9 
 
F.1.3 the toothbrushes shall be selected at random from the top, middle and bottom portion of the package. 
If the toothbrushes in a lot are pac ked in more than one package approximately equal number of brushes shall 
be selected at random from as many packages as possible so as to obtain the required number of 
toothbrushe s for tests, as given in Table F1  
F.1.4 for declaring the conformity of the l ot to the requirements of this specification, all the brushes selected 
according to F.1.2 and F.1.3 shall satisfy the relevant requirements for this standard  DUS  466: 2020  
© UNBS  2020 – All rights reserved  17 
 Bibliography  
[1] IS 3387:2004, Toothbrush — Specification  
[2] ISO 20126, Dentistry — Manual tooth brushes — General requirements and test methods    
[3] US 466:2006, Toothbrush — Specification   
 CDUS 466: 2020  
© UNBS 2020  – All rights reserved  19 
 Certification marking  
Products that conform to Uganda standards may be marked with Uganda National Bureau of Standards 
(UNBS) Certification Mark shown in the f igure below.  
The use of the UNBS Certification Mark is governed by the Standards Act, and the Regulations made 
thereunder. This mark can be used only by those licensed under the certification mark scheme operated by 
the Uganda National Bureau of Standards  and in conjunction with the relevant Uganda Standard. The 
presence of this mark on a product or in relation to a product is an assurance that the goods comply with the 
requirements of that standard under a system of supervision, control and testing in acc ordance with the 
certification mark scheme of the Uganda National Bureau of Standards. UNBS marked products are 
continually checked by UNBS for conformity to that standard.  
Further particulars of the terms and conditions of licensing may be obtained from the Director, Uganda 
National Bureau of Standards.  
 
 DUS 466: 2020    
 
ICS 97.170  
Price based on nn pages  
© UNBS 2020 – All rights reserved