ELI: http://data.europa.eu/eli/reg_impl/2024/220/oj 1/3COMMISSION IMPLEMENTING REGUL ATION (EU) 2024/220 
of 12 Januar y 2024
concer ning the renewal of the author isation of a preparation of Pediococcus pentosaceus NCIMB 
30168 as a feed additiv e for all animal species and amending Implementing Regulation (EU) 
No 1119/2012 
(Text with EEA relevance) 
THE EUR OPEAN COMMISSION,
Havi ng regar d to the Treaty on the Functioning of the European Union,
Havi ng rega rd to Regulation (EC) No 1831/2003 of the European Parliament and of the Council of 22 Sept ember 2003 on 
additives for use in animal nutr ition (1), and in particular Article 9(2) thereof,
Whereas:
(1) Regulation (EC) No 1831/2003 provid es for the author isation of additives for use in animal nutr ition and for the 
grounds and procedures for granting and renewing such author isation.
(2) The preparation of Pediococcus pentosaceus NCIMB 30168 was author ised for a period of 10 years as a feed additive for 
all animal species by Commission Implementing Regulation (EU) No 1119/2012 (2).
(3) In accordance with Article 14(1) of Regulation (EC) No 1831/2003, an application was submitted for the renewal of 
the author isation of the preparation of Pediococcus pentosaceus NCIMB 30168 as a feed additive for all animal species, 
requesting the additive to be classified in the additive catego ry ‘techn ological additives’ and in the functional group 
‘silag e additives’. That application was accompan ied by the particulars and documents required under Article 14(2) 
of that Regulation.
(4) The European Food Safet y Author ity (‘the Auth ority’) concluded in its opinion of 11 May 2023 (3)that the 
preparation of Pediococcus pentosaceus NCIMB 30168 remains safe for all animal species, the consumers and the 
environment under the conditions of use currently author ised, including the proposed use of a new cryoprotect ant 
in the formulation of the preparation. It also concluded that the additive should be considered a respirato ry 
sensitiser but no conclusions could be drawn on the skin sensitisation and on the skin and eye irritancy pote ntial of 
the additive. The Author ity also indicated that there is no need for assessing the efficacy of the additive in the cont ext 
of the renewal of the author isation.
(5) The Reference Laborator y set up by Regulation (EC) No 1831/2003 considered that the conclusions and 
recommendations reach ed in the assessment carried out regar ding the method of analysis of the preparation of 
Pediococcus pentosaceus NCIMB 30168 as a feed additive in the cont ext of the previous author isation are valid and 
applicable for the current application. In accordance with Article 5(4), point (c), of Commission Regulation (EC) 
No 378/2005 (4), an evaluation repor t of the Reference Laboratory is theref ore not required.
(1)OJ L 268, 18.10.2003, p. 29.
(2)Commission Implementing Regulation (EU) No 1119/2012 of 29 November 2012 concer ning the author isation of preparations of 
Pediococcus acidilactici CNCM MA 18/5M DSM 11673, Pediococcus pentosaceus DSM 23376, NCIMB 12455 and NCIMB 30168, 
Lactobacillus plantar um DSM 3676 and DSM 3677 and Lactobacillus buchner i DSM 13573 as feed additives for all animal species 
(OJ L 330, 30.11.2012, p. 14).
(3)EFSA Journal 2023;21(6):8046.
(4)Commission Regulation (EC) No 378/2005 of 4 March 2005 on detailed rules for the implement ation of Regulation (EC) 
No 1831/2003 of the European Parliament and of the Council as regards the duties and tasks of the Community Reference 
Laborato ry concer ning applications for author isations of feed additives (OJ L 59, 5.3.2005, p. 8).
Offi cial Jour nal  
of the European Union EN 
L series    
2024/220  15.1.2024ELI: http://data.europa.eu/eli/reg_im pl/2024/220/oj 2/3(6) In view of the above, the Commission considers that the preparation of Pediococcus pentosaceus NCIMB 30168 
satisfies the conditions provided for in Article 5 of Regulation (EC) No 1831/2003. According ly, the author isation 
of that additive should be renewed. In addition, the Commission considers that appropr iate protective measures 
should be taken to prevent adverse effects on the health of the users of the additive.
(7) As a consequence of the renewal of the author isation of the preparation of Pediococcus pentosaceus NCIMB 30168 as a 
feed additive, Implementing Regulation (EU) No 1119/2012 should be amended.
(8) The measures provid ed for in this Regulation are in accordance with the opinion of the Standing Committ ee on 
Plants, Animals, Food and Feed,
HAS ADOPTED THIS REGUL ATION:
Article 1
Renewal of the author isation
The author isation of the preparation specified in the Annex, belonging to the additive cate gory ‘technological additives’ and 
to the functional group ‘silag e additives’, is renewed subject to the conditions laid down in that Annex.
Article 2
Amendment to Implementing Regulation (EU) No 1119/2012
In the Annex to Implementing Regulation (EU) No 1119/2012, entr y 1k2107 on ‘Pediococcus pentosaceus NCIMB 30168’ is 
delet ed.
Article 3
Entr y into force
This Regulation shall enter into force on the twentieth day followi ng that of its publication in the Official Journal of the 
European Union.
This Regulation shall be binding in its entirety and directly applicable in all Member States.
Done at Brussels, 12 Januar y 2024 .
For the Commission
The President
Ursula VON DER LEYENOJ L, 15.1.2024 EN  ELI: http://data.europa.eu/eli/reg_impl/2024/220/oj 3/3ANNEX 
Identifica -
tion number 
of the 
additiveAdditiveComposition, chemical formula, 
descr iption, analytical methodSpecies or 
categ ory of 
animalMaximum 
ageMinimum 
conten tMaximum 
conten tOther provisi onsEnd of period of 
author isation
CFU/kg of fresh material
Categor y: technological additiv es. Functional group: silage additiv es
1k2107 Pediococcus 
pentosaceus NCIMB 
30168Additiv e composition
Preparation of Pediococcus pentosaceus 
NCIMB 30168 containing a minimum 
of 5 × 1010 CFU/g additive
Solid formAll animal 
species— — 1.In the directions for use of the additive and 
premixtures, the stora ge conditions shall be 
indicated .
2.Minimum dose of the additive when used 
without combination with other micro-or ­
gani sm as silage additive: 1 × 108 CFU/kg 
fresh mate rial.
3.For users of the additive and premixtures, feed 
business operat ors shall establish operational 
procedures and organisational measures to 
address poten tial risks resulting from their 
use. Where those risks cannot be eliminated 
by such procedures and measures, the addi ­
tive and premixtures shall be used with per­
sonal skin, eye and breathing prot ective 
equipment.4 Febr uary 
2034
Character isation of the activ e 
subst ance
Viable cells of Pediococcus pentosaceus 
NCIMB 30168
Analytical method (1)
Enumeration in the feed additive of 
Pediococcus pentosaceus NCIMB 30168:
— Spread plate method using MRS 
Agar (EN 15786)
Identif ication of Pediococcus pentosaceus 
NCIMB 30168:
— Pulsed Field Gel Electrophoresis 
(PFGE)
CEN/TS 17697 or DNA sequencing 
methods
(1)Details of the analytical methods are available at the following address of the Reference Laborato ry: https://joint-research-c entre.ec.europa.eu/eurl-fa-eurl-f eed-additives/eurl-fa-author isation/eurl-f a-evaluation- 
repor ts_enOJ L, 15.1.2024 EN