COMMISSION IMPLEMENTING REGUL ATION (EU) 2023/59 
of 5 Januar y 2023
concer ning the author isation of a preparation of Pediococcus pentosaceus DSM 32292 as a feed 
additiv e for all animal species 
(Text with EEA relevance) 
THE EUR OPEAN COMMISSION,
Havi ng regar d to the Treaty on the Functioning of the European Union,
Havi ng rega rd to Regulation (EC) No 1831/2003 of the European Parliament and of the Council of 22 Sept ember 2003 on 
additives for use in animal nutr ition (1), and in particular Article 9(2) thereof,
Whereas:
(1) Regulation (EC) No 1831/2003 provid es for the author isation of additives for use in animal nutr ition and for the 
grounds and procedures for granting such an author isation.
(2) In accordance with Article 7(1) of Regulation (EC) No 1831/2003, an application was submitte d for the 
author isation of a preparation of Pediococcus pentosaceus DSM 32292. That application was accompan ied by the 
particulars and documents required under Article 7(3) of Regulation (EC) No 1831/2003.
(3) The application concer ns the author isation of the preparation of Pediococcus pentosaceus DSM 32292 as a feed 
additive for all animal species, to be classified in the additive cate gory ‘technological additives’ and in the functional 
group ‘silag e additives’.
(4) The European Food Safet y Author ity (‘the Author ity’) concluded in its opinion of 29 June 2022 (2)that, under the 
proposed conditions of use, the preparation of Pediococcus pentosaceus DSM 32292 does not have an adverse effect 
on animal health, consumer safety or the environment. It also concluded that it should be considered a respirato ry 
sensitiser and note d that no conclusions could be drawn on its eye and skin irritancy or skin sensitisation potential. 
The Authority further concluded that the additive, at the proposed concentration, has the poten tial to improve the 
preser vation of nutr ients in silage prepared with moderately difficult to ensile mate rial. It also verified the repor t on 
the method of analysis of the feed additives in feed submitted by the Reference Laboratory set up by Regulation (EC) 
No 1831/2003.
(5) The assessment of the preparation of Pediococcus pentosaceus DSM 32292 shows that the conditions for author isation, 
as provided for in Article 5 of Regulation (EC) No 1831/2003, are satisfi ed. According ly, the use of the preparation 
should be author ised. The Commission considers that appropr iate prot ective measures should be taken to prevent 
adverse effects on human health, in particular as regar ds the users of the additive.
(6) The measures provid ed for in this Regulation are in accordance with the opinion of the Standing Committ ee on 
Plants, Animals, Food and Feed,
HAS ADOPTED THIS REGUL ATION:
Article 1
The preparation specifi ed in the Annex, belonging to the additive catego ry ‘techn ological additives’ and to the functional 
group ‘silag e additives’, is author ised as an additive in animal nutr ition, subject to the conditions laid down in that Annex.
(1)OJ L 268, 18.10.2003, p. 29.
(2)EFSA Jour nal 2022;20(7):7426.EN Offi cial Jour nal of the European Union L 5/16 6.1.2023  Article 2
This Regulation shall enter into force on the twentieth day followi ng that of its publication in the Official Journal of the 
European Union.
This Regulation shall be binding in its entirety and directly applicable in all Member States.
Done at Brussels, 5 Januar y 2023 .
For the Commission
The President
Ursula VON DER LEYENEN Offi cial Jour nal of the European Union 6.1.2023 L 5/17  ANNEX 
Identifica -
tion 
number of 
the additiveAdditiveComposition, chemical formula, descr iption, 
analytical methodSpecies or 
categ ory of 
animalMaximum 
ageMinimum 
conten tMaximum 
conten tOther provisi onsEnd of period of 
author isation
CFU/kg of fresh material
Categor y of technological additiv es. Functional group: silage additiv es
1k1018 Pediococcus 
pentosaceus DSM 
32292Additive composition:
Preparation of Pediococcus pentosaceus DSM 
32292 containing a minimum of 1 × 1010  
CFU/g additive
Char acter isation of the active substance:
Viable cells of Pediococcus pentosaceus DSM 
32292
Analytical method (1):
Enumeration in the feed additive: Spread 
plate method on MRS agar (EN 15786)
Identif ication: Pulsed-Field Gel 
Electrophoresis (PFGE) or DNA sequencing 
methodsAll animal 
species- - - 1.In the directions for use of the additive 
and premixtures, the storage conditions 
shall be indicate d.
2.Minimum content of the additive when 
used without combination with other mi­
cro-org anisms as silage additives: 5×107 
CFU/kg of easy and moderately difficult 
to ensile fresh mat erial(2).
3.For users of the additive and premixtures, 
feed business operators shall establish op­
erational procedures and organisational 
measures to address poten tial risks result ­
ing from its use. Where those risks cannot 
be eliminate d or reduced to a minimum 
by such procedures and measures, the ad­
ditive and premixtures shall be used with 
personal prot ective equipment, including 
skin, eye and breathing protect ion.26.1.2033
(1)Details of the analytical methods are available at the following address of the Reference Laborato ry: https://joint-research-c entre.ec.europa.eu/eurl-fa-eurl-f eed-additives/eurl-fa-author isation/eurl-f a-evaluation- 
repor ts_en .
(2)Easy to ensile forag e: > 3 % soluble carbohy drates in fresh material; moderat ely difficult to ensile forage: 1,5-3,0 % soluble carbohy drates in the fresh material, in accordance with Commission Regulation (EC) 
No 429/2008 of 25 Apr il 2008 on detailed rules for the impl ementation of Regulation (EC) No 1831/2003 of the European Parliament and of the Council as regards the preparation and the presentation of 
applications and the assessment and the author isation of feed additives (OJ L 133, 22.5.2008, p. 1).EN Offi cial Jour nal of the European Union L 5/18 6.1.2023