DEAS 1075 :2021  
ICS 59 .080.30  
 
© EA C 2021                                                                                                                           First Edition 2021   
 DRAFT EAST AFRICAN STANDARD  
Disposable wet wipes — Specification  
EAST AFRICAN COMMUNITY  
 
 
 
  DEAS  1075: 2021  
ii © EAC 2021  – All rights reserved  
 Copyright notice  
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participants in the EAC standards development process is permitted without prior permission from EAC, 
neither this document nor any extract from it may be reproduced, stored or transmitted in any form for 
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© East African Community  2021 — All rights reserved  
East African Community   
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Tanzania  
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Reproduction for sales purposes may be subject to royalty payments or a licensing agreement.  
Violators may be prosecuted .  DEAS  1075: 2021  
© EAC 2021  – All right s reserved  iii 
 Contents  Page 
Foreword  ................................ ................................ ................................ ................................ ............................  iv 
Introduction  ................................ ................................ ................................ ................................ .........................  v 
1 Scope  ................................ ................................ ................................ ................................ ......................  1 
3 Terms and definitions  ................................ ................................ ................................ ...........................  1 
4 Requirements  ................................ ................................ ................................ ................................ .........  2 
4.1 General  ................................ ................................ ................................ ................................ ...................  2 
4.2 Specific requirements  ................................ ................................ ................................ ...........................  2 
4.2.1  Fibre composition  ................................ ................................ ................................ ................................ . 2 
4.2.2  Dimensions  ................................ ................................ ................................ ................................ ............  2 
4.2.3  Performance requirements  ................................ ................................ ................................ ...................  3 
4.2.4  Chemical requirements  ................................ ................................ ................................ .........................  3 
4.2.5  Microbiological requirements  ................................ ................................ ................................ ..............  3 
4.2.6  Active ingredient for sanitizing wipes  ................................ ................................ ................................ . 4 
4.2.7   Biocompatibility  ................................ ................................ ................................ ................................ .... 4 
5 Packaging ................................ ................................ ................................ ................................ ...............  4 
6 Labelling  ................................ ................................ ................................ ................................ .................  4 
6.1 Primary packaging  ................................ ................................ ................................ ................................  4 
6.2. Secondary packaging ................................ ................................ ................................ ...........................  5 
7 Sampling  ................................ ................................ ................................ ................................ ................  5 
Annex  A (normative)  Determination of moisture content  ................................ ................................ .............  6 
A.1 Principle ................................ ................................ ................................ ................................ .................  6 
A.2 Apparatus ................................ ................................ ................................ ................................ ...............  6 
A.3 Sample  preparation ................................ ................................ ................................ ...............................  6 
A.4 Procedure ................................ ................................ ................................ ................................ ...............  6 
A.5 Calculations  ................................ ................................ ................................ ................................ ...........  7 
Annex  B (normative)  Determination of flushability  ................................ ................................ .......................  8 
Annex  C (normative)  Determination of disinfecting efficacy  ................................ ................................ ........  9 
C.1 Outline of the method  ................................ ................................ ................................ ...........................  9 
C.2 Apparatus  ................................ ................................ ................................ ................................ ...............  9 
C.3 Media ................................ ................................ ................................ ................................ .......................  9 
C.3.1  Growth media for test organisms  ................................ ................................ ................................ ........  9 
C.3.2  Recovery medium  ................................ ................................ ................................ ................................  10 
C.3.3  Hard water  ................................ ................................ ................................ ................................ ............  10 
C.3.4  Yeast suspension  ................................ ................................ ................................ ................................  10 
C.3.5  Ringers solution, 25 % (v/v)  ................................ ................................ ................................ ...............  11 
C.4 Selection of the most resistant organism by the minimum inhibitory concentration test  ..........  11 
C.5 Preparation of inoculum  ................................ ................................ ................................ .....................  11 
C.6 Preparation of the sanitizer dilutions  ................................ ................................ ................................  12 
C.7 Test procedure  ................................ ................................ ................................ ................................ ..... 12 
C.8 Inter pretation of results  ................................ ................................ ................................ ......................  12 
Bibliography  ................................ ................................ ................................ ................................ ......................  13 
 
  DEAS  1075: 2021  
iv © EAC 2021  – All rights reserved  
 Foreword  
Development of the East African Standards has been necessitated by the need for harmonizing requirements 
governi ng quality of products and services in the East African Community. It is envisaged that through 
harmonized standardization, trade barriers that are encountered when goods and services are exchanged 
within the Community will be removed.  
The Community has established an East African Standards Committee  (EASC)  mandated to develop and 
issue East African Standards  (EAS) . The Committee is composed of representatives of the National 
Standards Bodies in Partner States, together with the representatives from the public and private sector 
organizations  in the community .  
East African Standards are developed through  Technical Committees that are representative of key 
stakeholders including government, academia, consumer groups, private sector and other interested part ies. 
Draft East African Standards are circulated to stakeholders through the National Standards Bodies in the 
Partner States. The comments received are discussed and incorporated before finalization of standards, in 
accordance with the Principles and proce dures for development of East African  Standards.  
East African Standards are subject to review, to keep pace with technological advances. Users of the East 
African Standards are therefore expected to ensure that they always have the latest versions of the s tandards 
they are implementing .  
The committee responsible  for this document is Technical Committee EASC/TC 061, Textiles, textile products 
and accessories . 
Attention is drawn to the possibility that some of the elements of this document may be subject of patent 
rights. EAC shall not be held responsible for identifying any or all such patent rights.   DEAS  1075: 2021  
© EAC 2021  – All right s reserved  v 
 Introduction  
Non-woven wet wipes are disposable sanitary products  used  for light rubbing in order to remove dirt and 
germs on the skin. In this standard, wet wi pes have been classified into personal hygiene and sanitizing wet 
wipes based on their intended use. Personal hygiene wet wipes are used for baby or adult hygiene (hand, 
facial, mucous membrane) cleansing purposes. Sanitizing wipes have a double benefit of  providing the  
sanitizing solution used for killing germs as well as removing dirt, grease and other particles off the skin.  
 DRAFT EAST AFRICAN STANDARD  DEAS  1075: 2021  
 
© EAC 2021  – All rights reserved  1 
 Disposable wet wipes — Specification  
1 Scope  
This Draft East African Standard specifies requirements, sampling and test methods  for non -woven 
disposable wet wipes applicable for general personal hygiene and sanitizing purposes  
2 Normative references   
The following documents are referred to in the text in such a way that some or all of their content constitutes 
requirements of this  document.  For dated references, only the edition cited applies. For undated references, 
the latest edition of the referenced document (including any amendments) applies.  
EAS 377 -1, Cosmetics and cosmetic products — Part 1: List of substances prohibited in  cosmetic products  
EAS 377 -2, Cosmetic and cosmetic products — Part 2: List of substances which cosmetic products must not 
contain except subject to the restrictions laid down   
EAS 377 -4, Cosmetics and cosmetics products — Part 4: List of preservatives all owed in cosmetic products  
ISO 1833 -11, Textiles — Quantitative chemical analysis — Part 11: Mixtures of certain cellulose fibres with 
certain other fibres (method using sulfuric acid)  
ISO 3071, Textile materials; Method for determination of pH value of aqu eous extracts   
ISO 9073 -1, Test methods for nonwovens — Part1: Determination of mass per unit area   
ISO 9073 -18, Test methods for nonwovens — Part 18: Determination of breaking strength and elongation of 
nonwoven materials using the grab tensile test . 
ISO 18416, Cosmetics — Microbiology — Detection of Candida albicans   
ISO 20743, Textiles — Determination of antibacterial activity of textile products   
ISO 21149, Cosmetics — Microbiology — Enumeration and detection of aerobic mesophilic bacteria   
ISO 21150, Cosmetics — Microbiology — Detection of Escherichia coli   
ISO 22198, Textiles — Fabrics — Determination of width and length  
ISO 22717, Cosmetics — Microbiology — Detection of Pseudomonas aeruginosa   
ISO 22718, Cosmetics — Microbiology — Detection of Staphyl ococcus aureus   
3 Terms and definitions  
For the purposes of this document, the following terms and definitions shall apply.  
ISO and IEC maintain terminological databases for use in standardization at the following addresses:  DEAS  1075: 2021  
2 © EAC 2021  – All rights reserved  
 — ISO Online browsing platfor m: available at http://www.iso.org/obp  
3.1  
non-woven  
structures of textile materials, such as fibres, continuous filaments, or chopped yarns of any nature or origin, 
that have been formed into webs by any means, and bonded together by any means, excludin g the interlacing 
of yarns as in woven fabric, knitted fabric, laces, braided fabric or tufted fabric  
3.2  
wipe  
disposable non -woven material treated with a cleansing or sanitizing agent , rubbed on the skin to remove dirt 
or germs  
3.3  
active ingredient  
chemical and/or biological  component that is included in the formulation of cleaning/sanitizing agents used to 
treat wipes to achieve their intended purposes  
4 Requirements  
4.1 General  
Disposable wet wipes shall : 
a) be in accordance with good manufacturing pr actices;  
b) be of a non -woven construction;  
c) be of acceptable uniform make and finish;  
d) in case of general personal hygiene wet wipes, they shall be pre -moistened with a non -toxic, 
hypoallergenic and alcohol free cleansing solution that is completely air dried when applied onto the 
skin; 
e) in case of sanitizing wipes, they shall be pre -soaked with a non -toxic and hypoallergenic concentrated 
sanitizing solution  
f) be made from ingredients complying with EAS 377 -1, EAS 377 -2 and EAS 377 -4; 
g) leave no lint on the wiped s urface, be free from any foreign matter or any other defect that mi ght 
impair  their appearance or serviceability  
4.2 Specific r equirements  
4.2.1  Fibre composition  
When tested in accordance with ISO 1833 -11, non -woven disposable wet wipes shall consist of a t least 20 % 
cellulose fibres.  
4.2.2  Dimensions  
When te sted in accordance with ISO 22198 the nomi nal dimensions of the wipes  shall be as declared subject 
to a tolerance of ± 2 % of the declared dimensions  DEAS  1075: 2021  
© EAC 2021  – All right s reserved  3 
 4.2.3  Performance requirements  
Personal hygiene an d sanitizing wet wipes shall comply with the performance requirements given in Table 1 
when tested in accordance with the test methods specified therein.  
Table 1 — Performance requirements for disposable wet wipes  
S/N Parameter  Requirement  Test method  
i Mass per unit area, g/m2, min.  36 ISO 9073 -1 
ii aBreaking strength, 
N, min.  Machine direction (Wet)  30 ISO 9073 -18 
Cross direction (Wet)  2.5 
iii pH of extracted cleansing solution  4.5 – 8.5 ISO 3071  
iv Moisture content, %, min.  50 Annex A 
v b Flusha bility Pass the test  Annex B 
a Immerse each sample before testing for at least 60 s in distilled water and test each specimen 
immediately after removal from the water.  
b Flushability test shall be subjected to wipes that are declared as flushable  
 
Note  Continuous flushing of wipes is  liable to cause clogging of sewer systems  over time . It is  advisable  for 
manufacturers to include warnings for flushable wipes.  
4.2.4  Chemical requirements  
Personal hygiene and sanitizing wet wipes shall comply with the chemi cal requirements given in Table 2 when 
tested in accordance with the test methods specified therein.  
Table 2 — chemical requirements for disposable wet wipes  
S/N Parameter  
mg/kg, max.  Requirement  Test method  
i Lead,  10 EAS 786  
ii Arsenic  2 
iii Mercury  2 
iv Cadmium  5 
v Chlorinated organic compounds  150 ISO 11480  
 
4.2.5  Microbiological requirements  
Personal hygiene and sanitizing wet wipes shall comply with the microbiological requirements given in Table 3 
when tested in accordance with the test met hods specified therein.  
Table 3 — microbiological requirements for disposable wet wipes  
S/N Parameter  Requirement  Test method  
Personal hygiene wipes  Sanitizing wipes  
i Anti-bacterial activity (A) (if 
declared), min.  2 ISO 20743  DEAS  1075: 2021  
4 © EAC 2021  – All rights reserved  
 ii Total viable count,  cfu/ 1g or 1 
mL, max.  100 Not detected  ISO 21149  
iii Pseudomonas aeruginosa, cfu/g  Not detected  ISO 22177  
iv Staphylococcus aureus , cfu/g  Not detected  ISO 22718  
v Candida albicans , cfu/g  Not detected  ISO 18416  
vi Escherichia coli , cfu/g  Not detected  ISO 21150  
 
4.2.6 Active ingredient for sanitizing wipes  
Sanitizing wipes shall  conform to the active ingredient requirements specified in Table 4  when tested in 
accordance with the test methods prescribed therein.  
Table 4  — Requirements of active ingredien ts 
S/N Parameter  Requirement  Test method  
i aAlcohol content (ethanol and/or isopropanol, 
n-propanol), %, v/v, min.  60.0 EAS 104  
ii Bacterial efficacy  To pass test  Annex C  
a Alcohol is the most commonly used active agent. However, other suitable disinfec ting 
agents in appropriate concentrations may be used as substitute agents.  
 
4.2.7   Biocompatibility  
When tested in accordance with the relevant parts of ISO 10993, the sanitizing wipes shall not cause any 
harmful effect to the skin.  
5 Packaging  
5.1 Disposable wet wipes shall be packaged in a suitable resealable material presenting no defects that 
can affect its serviceability  
5.2 The primary packaging shall be designed to easily dispense a single towelette at a time keeping the 
remaining towelettes unexpo sed to contamination and prevent loss of the moisture or other active ingredients  
5.3 Only wipes of the same size shall be packed together in both primary and secondary packages.  
6 Labelling  
6.1 Primary packaging  
a) The primary packaging shall be legibly and indelibly labelled either in English, Kiswahili or French 
or a combination with the following information:  
a) manufacturer’s name, address and/or trade mark;  
b) importer/distributors name, address (if applicable);  
c) product name and intended use such as “ baby wet wipes” , “adult wet wipes”  or “sanitizing/disinfection 
wipes”  DEAS  1075: 2021  
© EAC 2021  – All right s reserved  5 
 d) for sanitizing wipes, the active ingredient used and its percentage composition  
e) if anti -bacterial, the declaration, “Anti -bacterial”  
f) number of wipes in a pack age; 
g) size of wipe s in the pack age; 
h) fibre composition;  
i) list of ingredients used ; 
j) instruction for use, storage and disposal;  
k) date of manufacture and expiry; or best before;  
l) batch number; and  
m) country of origin /manufacture ; 
6.2. Secondary packaging 
The outside of each secondary package shall be legibly and indelibly labelled either in English, Kiswahili or French 
or a combination with the following information: 
a) manufacturer’s name, address and/or registered trade mark; 
b) product name and intended use such as “baby wet wipes”, “adult wet wipes” or “ sanitizing/disinfection 
wipes”  
c) number of packages;  
d) date of manufacture and expiry ; 
e) batch  or lot  number; and  
f) country of origin /manufacture . 
7 Sampling  
Random samples of the product shall be drawn for test in accordance with ISO 2859 -1. DEAS  1075: 2021  
6 © EAC 2021  – All rights reserved  
 Annex  A 
(normative ) 
 
Determination of moisture content  
A.1 Principle 
A specimen of specified mass of filler material of the non-woven disposable wet wipe is dried in an oven at 
specified temperature and the moisture content is determined. 
A.2 Apparatus 
A.2.1 Balance, with an accuracy of 0.05 % of t he weighed mass. 
A.2.3 Sample container, waterproof when sealed, will be used for transfer of analyzed material and 
during weighing. 
A.2.4 Oven, well ventilated with a temperature of 102 ºC to 105 ºC. 
A.3 Sample  preparation 
A.3.1 Take a sufficient number of dry sample containers, number them and take their masses after they 
are held open for a short period of time so that they will have the same air pressure as  the surrounding 
atmosphere. Then leave them o pen until you take the test piece. 
A.3.2 Take 5 random pieces of the wet wipe. The test pieces shall weigh 5 g. 
A.3.3 If the surrounding atmosphere is hot and humid, prevent water condensation on the internal and 
external  surfaces of the container. 
A.3.4 Handle the  test pieces gently to prevent dirt or changes in water content. Do not touch the test pieces 
with your bare hands. Put the test pieces in a container just after taking them and close the container 
immediately. 
A.4 Procedure  
A.4.1 Dry the test pieces in an oven with a temperature of 102 ºC to 105 ºC. Open the containers lid and 
dry the specimen inside the container. Open the container for a moment, to balance the air pressure inside 
the container  with the  surrounding pressure, weigh  the  container that  holds  the  specimen again  and 
calculate the weight of the specimen. 
A.4.2 First cycle of drying will last at least 30 min. Return the container with the test pieces to the oven, for 
at least half the first cycles drying time. Take the  container out and take the  mass with the test pieces inside. 
Repeat the drying and weighing cycles. When the drying time on every cycle is at least half of the total 
previous drying cycle times. Continue the process until the difference between  two consecutive masses does 
not exceed 0.1 % of t he original mass of the specimen. DEAS  1075: 2021  
© EAC 2021  – All right s reserved  7 
 A.5 Calculations  
Calculate the moisture content using the following formula and round the results up to the nearest 0.1 %. 
 
where,  
a is weight of the container with the specimen before drying (in grams); 
b is weight of the container with the specimen after drying (in gra ms); and  
V is water content (in weight %)  DEAS  1075: 2021  
8 © EAC 2021  – All rights reserved  
 Annex  B 
(normative ) 
 
Determination of flushability  
B.1 Apparatus   
Use the flushing toilet equipment as described in Figure B.1. The pipes to the septic tank should be  
transparent acryl tubes to allow the sanitary end of the tube to an open tank so as to collect the products.   
B.2 Test method   
Throw three wipes into the chamber pot, and flush water (8 to 12 litres per time) from the flush tank.   
Dimensions in millime tres  
  
B.3 Test report   
Report whether the wipes  are completely flushed down or not.   
 DEAS  1075: 2021  
© EAC 2021  – All right s reserved  9 
 Annex  C 
(normative ) 
 
Determination of disinfecting efficacy  
C.1 Outline of the method  
C.1.1 A sanitizing wipe is pressed to obtain the sanitizing solution. The sani tizing solution  is tested at the 
recommended ‘use -dilution‘ and concurrently at 0.5 and 1.5 times that dilution. The test consists of 
challenging the diluted sanitizer with bacterial inoculum, withdrawing a sample after a given time and culturing 
the sampl e in a suitable recovery medium.  After this sampling, the mixture is again challenged by a second 
inoculum and after a second interval, is again sampled for culturing. This process is then repeated to provide 
a third challenge.  
C.1.2 The sample is consid ered to have passed or failed the test according to the extent of growth shown in 
the first two cultured samples.  
C.2 Apparatus  
C.2.1  Facility, for incubation  at 37 °C ± 1 °C  
C.2.2  Facility, for incubation  at 2 °C ± 1 °C  
C.2.3  Stop clock , indicating  in seconds  
C.2.4  Facility,  for refrigeration  at 4 °C ± 1 °C  
C.2.5 Universal containers , made of glass and having metal tops with rubber liners. Plastic containers or 
glass containers with plastic tops shall not be used.  
C.2.6  Test tubes , 19 mm X 150 mm  
C.2.7  Filter paper , No. 4 Whatman (sterile)  or equivalent  
C.2.8  Facility , for autoclaving  at 121 °C ± 1 °C  
C.2.9  Pipette , capable of dispensing 0.02 mL ± 0.005 mL  
C.2.10  pH meter   
C.2.11  Facility , to sterilize  by filtration  
C.2.12  150 μm test sieve   
C.2.13  Oven , capable of maintaining temperature at 100 °C ± 1 °C  
C.3 Media  
C.3.1 Growth media for test organisms  
C.3.1.1   The growth media for test organisms shall be Wright and Mundy Broth with Dextrose (WMBD).  DEAS  1075: 2021  
10 © EAC 2021  – All rights reserved  
 C.3.1.2  Dispense 10 m L and 6 mL quantities of the Wright and Mundy Broth into universal bottles, and 
autoclave at 121 °C ± 1 °C for 12 min.  
C.3.1.3  Add to this medium, 10 % (m/V) dextrose solution sterilized by filtration, to give a final dextrose 
concentration of 0.1 % (m/v) , (that is, to 10 mL broth add 0.1 mL dextrose solution and to 6.0 mL broth add 
0.06 mL dextrose solution).  
C.3.2 Recovery medium  
C.3.2.1  Composition  
A nutrient broth prepared as follows:  
a) beef extract, 10 g;  
b) peptone, 10 g;  
c) sodium chloride, 5 g; and  
d) polyoxyethylene sorbitan mono -oleate, 30 g  
C.3.2.2  Preparation  
Add the ingredients to 1 000 mL of water.  Mix well.  Dispense 10 mL quantities into test tubes and autoclave 
at 121 °C ± 1 °C for 15 min .  
C.3.3 Hard water  
Standard hard water with 342 mg/L  (ppm) hardness prepared as follows: dissolve 0.304 g of anhydrous 
calcium chloride hexahydrate (MgCl 2.6H 20) in distilled water and make up the volume to one litre.  Sterilize the 
standard hard water by autoclaving at 121 °C ± 1 °C for 15 min. Allow this t o reach room temperature before 
use.  
C.3.4 Yeast suspension  
C.3.4.1  Weigh to the nearest gram about 65 g of active dry yeast.  Cream by the gradual a ddition of sterile 
hard water (C .3.3) using a heavy glass rod for stirring.  Decant the creamed portion i nto a flask, add more 
hard water to any lumpy residue remaining and repeat the creaming and decantation until no residue 
remains, and 500 mL of hard water has been used.  
C.3.4.2  Shake the contents of the flask vigorously and strain -through a 150 μm sieve (C .2.12) breaking 
down any remaining lumps.  
C.3.4.3   Add 500 mL sterile hard water, shake vigorously.  
C.3.4.4  Transfer 50 mL or 100 mL portions into screw -capped bottles, screw the caps tightly and 
autoclave at 121 °C ± 1 °C for 15 min.  Allow the autoclave to cool without releasing the pressure. Store cold 
but not freezing.  
C.3.4.5  Dry two glass petri -dishes to constant mass.  Into each of these dishes, pipette 25 mL of sterilized 
yeast suspension and dry to constant mass at 100 °C .  Calculate the average solids content of the 
suspension.  
C.3.4.6  Before use, pipette 25 mL of the sterilized yeast suspension into a beaker.  Determine the pH 
using a glass electrode, and determine the volume of 40 g/L sodium hydroxide solution needed t o adjust the 
pH to 7.0 ± 0.1.  DEAS  1075: 2021  
© EAC 2021  – All right s reserved  11 
 C.3.4.7  Immediately before use, add to each bottle of sterilized yeast suspension a volume of sterile hard 
water and a volume of 40 g/L sodium hydroxide calculated to adjust the concentration of dry yeast to 5 % 
(m/v) and the  pH to 7.0 ± 0.1.  Discard prepared yeast, two weeks after preparation.  
C.3.5 Ringers solution, 25 % (v/v)  
Dissolve 9.00 g of sodium chloride, 0.42 g of potassium chloride, 0.24 g of anhydrous calcium chloride and 
0.20 g of sodium bicarbonate in water and  dilute to 1 000 mL. Add one volume of this solution to three 
volumes of water to give a 25 % solution.  Dispense into test tubes fitted with suitable closures and sterilized 
by auto -claving at 121 °C ± 1 °C for 15 min .  
C.4 Selection of the most resistant  organism by the minimum inhibitory 
concentration test   
C.4.1  The following organisms shall be used for the test:  
a) Pseudomonas aeruginosa (NCTC 6749 or equivalent);   
b) Proteus vulgaris  (NCTC 4635 or equivalent); and   
c) Staphyloccus aureus (NCTC 4163 or equ ivalent).  
These organisms may be obtained as freeze dried cultures. Once sub -cultured, the organisms shall be 
maintained on agar slopes of suitable nutrient medium at 4 °C ± 1 °C.  
C.4.2 Subculture each organism daily into a universal bottle contain ing 6 mL of growth medium (see C .3.1) 
and incubate for 24 h ± 2 h at 37 °C ± 1 °C.  
C.4.3 Dilute one part of freshly grown sub -culture of each organism, which is at least a fifth sub -culture and 
not more than a fourteenth, with ten p arts of the growth medium (se e C.3.1) before dilution, the P. aeruginosa , 
culture shall be filtered using a Whatman No.4 filter paper.  
C.4.4 Prepared three sets of ten, doubling dilutions of the sani tizer in universal containers (C .2.5).  For this 
purpose dilute the neat sanitizer in  the growth medium (see C.3.1) or the recovery medium (C .3.2) to give a 
final volume of 5 mL of the diluted sanitizer for each dilution.  
C.4.5  Inoculate each dilution in one set with 0.02 mL of a dilute d culture of one organism (see C .4.3).  
C.4.6 Incuba te all the three sets of inoculate dilutions at 37 °C ± 1 °C for 72 h, and examine to determine 
the organism most resistant to the sanitizer, that is the organism for which the minimum inhibitory 
concentration is highest.  
C.5 Preparation of inoculum  
C.5.1 Daily sub -cultures of the test organism selected as in A.4.6 shall be grown in 6 mL qu antities of the 
growth medium (C .3.1) and incubated at  37 °C ± 1 °C for 24 h ± 2 h.  
C.5.2 The day before the test, inocula te 10 mL of the growth medium (C .3.1) with t he test organism from a 
daily sub -culture and not more than a fourteenth.  Incubate the inoculated, broth at 37 °C ± 1 °C for 24 h ± 2 h.  
C.5.3 Add 6 mL  of the test organism culture (C.5.1) and (C .5.2) to  4 mL of the yeast suspension (C .3.4) thus 
making a  final concentration of 2 % (m/v) of yeast in the yeast/organism suspension.  If a culture of P. 
aeruginosa  is used, it shall be filtered using a Whatman No.4 filter paper before addition.  DEAS  1075: 2021  
12 © EAC 2021  – All rights reserved  
 C.5.4 Shake the yeast/organism suspension for one minute with a fe w sterile glass beads. Immediately 
before the test, count the number of viable organisms in the inoculum by decimal dilutions in 25 % Ringers 
solution (see C .3.5) and by the drop plate method.  The viable count shall be not less than 108 organisms/mL 
or mo re than 1010 organisms/mL or the test results are considered invalid.  
C.6 Preparation of the sanitizer dilutions   
Prepare three dilutions o f the sanitizer in hard water (C .3.3) based on the recommended ‘use dilution‘ of the 
sanitizer, as follows:   
• A = 0 .5 times the recommended ‘use -dilution‘;  
• B = 1.0 times the recommended  ‘use -dilution‘; and  
• C = 1.5 times the recommended  ‘use -dilution‘.  
The sanitizer dilutions shall be prepared and tested on the same day.   
C.7 Test procedure   
C.7.1  The test shall  be carried out at 27 °C ± 1 °C.   
C.7.2 Dispense 3 mL of each dilution of sanitizer (C. 6) into separate universal bottles labelled A, B, and C, 
then allow to equilibrate to 27 °C ± 1°C.   
C.7.3  Add 1 mL of the inoculum to A, B and C at 0, 1 and 5 min res pectively and mix by swirling gently.   
C.7.4 Eight minutes after the addition of the inoculum, remove a sample of the inoculum/sanitizer mixture 
and put 0.02 mL into each of the first group of five tubes of recovery broths. Return the remainder of the 
mixture in the pipette to the universal container.  
C.7.5 Ten minutes after the first addition of the inoculum, add another 1 mL of the inoculum to each of the 
sanitizer dilutions and mix by swirling gently  
C.7.6 After 8 min, remove a sample  of the mixture a s put before (C .7.4) and put 0.02 mL into each of the 
second group of five tubes of recovery broths.  
C.7.7 Twenty minutes after the first addition of the inoculum, add a further 1 mL of inoculum to each of the 
sanitizer dilutions and mix by swirling gentl y.  
C.7.8 After 8 min, remove a sample of the mixture as before and place 0.02 mL into each of the third group 
of five tubes of recovery broths.  
C.7.9  Swirl the recovery broths and incubate at 37 °C ± 1 °C for 48 h ± 2 h.  Examine the growth and record 
the results.  
C.8 Interpretation of results  
C.8.1 The sanitizing wipe , shall be regarded as having passed the test at the recommended ‗use dilution‘ if 
there is no growth in at least two of the five recovery broths for the first and second additions of the  inoculum.  
C.8.2 To be accep table, the sanitizing wipe  shall pass the test on three separate occasions using freshly 
prepared sanitizing solution  and freshly prepared inoculum on each occasion.  DEAS  1075: 2021  
© EAC 2021  – All right s reserved  13 
 Bibliography  
[1] FDKS 2720:2021, Non -woven disposable wet wip es — Specification  
[2] DRS 439:2020, Non-woven skin care wet wipes – Specification  
 DEAS  1075: 2021    
 
© EAC 2021  – All rights reserv ed