DRAFT UGANDA STANDARD  
  DUS 170 
Second  Edition  
2018 -mm-dd 
 
 
 Reference number  
DUS 170: 2018 
© UNBS 2018   
  
Edible Cotton Seed Oil — Specification  
 DUS 170: 2018  
ii © UNBS 2018  – All rights reserved  
 Compliance with this standard does not, of itself confer immunity from legal obligations  
A Uganda Standard does not purport to include all necessary provisions of a contract. Users are 
responsible for its correct a pplication  
© UNBS 2018   
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilised in any 
form or by any means, electronic or mechanical, including photocopying and microfilm, without prior written 
permission  from UNBS.  
Requests for permission to reproduce this document should be addressed to  
The Executive Director  
Uganda National Bureau of Standards  
P.O. Box 6329  
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Uganda  
Tel: +256 41 4 333 2 50/1/2/3  
Fax: +256 41 4 286 123  
E-mail: info@unbs.go.ug  
Web: www.unbs.go.ug  DUS 170:2018  
© UNBS 2018  – All rig hts reserved  iii 
 Contents  Page 
Foreword  ................................ ................................ ................................ ................................ ............................  iv 
1 Scope  ................................ ................................ ................................ ................................ ......................  1 
2 Normative references  ................................ ................................ ................................ ............................  1 
3 Terms and definitions  ................................ ................................ ................................ ...........................  2 
4 Compositi onal and Quality Requirements  ................................ ................................ ..........................  2 
4.1 Quality Requirements  ................................ ................................ ................................ ...........................  2 
4.2 Fortification  ................................ ................................ ................................ ................................ ............  3 
4.3 Food additives  ................................ ................................ ................................ ................................ ....... 3 
4.4 Hygiene  ................................ ................................ ................................ .............. Error! Bookmark not defined.  
4.5 Chemical and Physical characteristics  ................................ ................... Error! Bookmark not defined.  
4.6 Contaminants  ................................ ................................ ................................ ................................ .........  3 
4.7 Pesticide residues  ................................ ................................ ..................... Error! Bookmark not defined.  
5 Packaging  ................................ ................................ ................................ ................................ ...............  3 
6 Labelling  ................................ ................................ ................................ ................................ .................  3 
7 Sampling  ................................ ................................ ................................ ................................ ................  4 
Annex  A (normative)  GLC Fatty acid composition  ................................ ............ Error! Bookmark not defined.  
A.1 General  ................................ ................................ ................................ .......Error! Bookmark not defined.  
Annex  B (informative)  COTTON SEED OIL (HALPHEN) TEST  .......................... Error!  Bookmark not defined.  
Bibliography  ................................ ................................ ................................ ................................ ........................  5 
 DUS 170: 2018  
iv © UNBS 2018  – All rights reserved  
 Foreword  
Uganda National Bureau of Standards (UNBS) is a parastatal under the Ministry of Trade , Industry and 
Cooperatives established under Cap 327 , of the Laws of Uganda , as amended .  UNBS is mandated to co -
ordinate the elabora tion of standards and is  
(a) a member of International Organisation for Standardisation (ISO) and  
(b) a contact point for the WHO/FAO Codex Alimentarius Commission on Food Standards, and  
(c) the National Enquiry Point on TBT  Agreement  of the World Trade Organisatio n (WTO).  
The work of preparing Uganda Standards is carried out through Technical Committees. A Technical 
Committee is established to deliberate on standards in a given field or area and consists of key stakeholders 
including government, academia, consumer groups, private sector and other interested parties.  
Draft Uganda Standards adopted by the Technical Committee are widely circulated to stakeholders and the 
general public for comments. The committee reviews the comments before recommending the draft stan dards 
for approval and declaration as Uganda Standards by the National Standards Council.  
The committee responsible for this document is Technical Committee UNBS /TC2, Subcommittee SC 2, on 
Edible oil seeds, fats and oils  
This second edition cancels and replaces the first edition ( US170:2000 ), which has been technically revised .  
 DRAFT UGANDA STANDARD  DUS 170: 2018  
 
© UNBS 2018  – All rights reserved  1 
 Cotton Seed Oil — Specification  
1 Scope   
This Draft Uganda Standard specifies the requirements , sampling and test  for crude and refined cotton oil 
derived from Cottonseeds  (gossypium )  
2 Normative references   
The following referenced documents referred to in the text in such a way that some or all of their content 
constitutes requirements of this document. For dated references, only the edition cited applies. For undated 
references, the latest edition of the referenced document (including any amendments) applies.  
US EAS 38 , Labelling of pre -packaged foods  — Specification  
US EAS 39 , Code of practice for hygiene for food and drink industries  
US ISO 660, Animal and vegetable fa ts and oils  Determination of acid value and acidity  
US ISO 662, Animal and vegetable fats and oils  Determination of moisture and volatile matter  content  
US ISO 663, Animal and vegetable fats and oils  Determination of insoluble impurities  content US IS O 3596, 
Animal and vegetable fats and oils  Determination of unsaponifiable matter  — Method using diethyl ether  
US ISO 3657, Animal and vegetable fats and oils  Determination of saponification value  
US ISO 3960, Animal and vegetable fats and oils  Deter mination of peroxide value  — Iodometric (visual) 
endpoint determination  
US ISO 3961, Animal and vegetable fats and oils  Determination of iodine value  
US ISO 5508: Animal and vegetable fats and oils — Analysis by gas chromatography of methyl esters of fat ty 
acids  
US ISO 5555, Animal and vegetable fats and oils  Sampling  
US ISO 6320, Animal and vegetable fats and oils  Determination of refractive index  
US ISO 6321,  Animal and vegetable fats and oils — Determination of melting point in open capillary tubes  
(Slip point)  
US ISO 8294, Animal and vegetable fats and oils  Determination of copper, iron and nickel  contents — 
Graphite furnace atomic absorption method  
US ISO 12193, Animal and vegetable fats and oils  Determination of lead  by direct  graphite furnac e atomic 
absorption spectroscopy  
US ISO 15305, Animal and vegetable fats and oils  Determination of Lovibond colour   
2 © UNBS 2018  – All rights reserved  
 IUPAC 2.101, Determination of Relative Density  
3 Terms and definitions   
For the purposes of this document, the following terms and definiti ons apply.  
3.3 
Crude cotton seed  oils, 
oils of plant origin obtained by mechanical procedures and/or solvent extraction only  that have been purified 
by washing, settling, filtering and centrifuging only.  
3.4 
Non –crude  (refined) cotton seed  oils 
oils of p lant origin obtained by mechanical procedures and/or solvent extraction and subjected to refining 
processes to adopt it specially for use in food products.  
4 Requirements  
4.1 General Requirements  
Cottonseed oil shall  be 
a) of colour characteristic of the des ignated product;  
b) free from adulterants, sediments, suspended or foreign matter, separated water and added colouring 
or flavouring substances;  
c) free from foreign or rancid odour and taste.  
d) obtained from good quality, clean and sound cotton seed by the proces s of expression  
4.5 Specific requirements  
Cottonseed oil shall have the specific characteristics shown in Tables 1.  
Table 1  —Specific requirements of Cotton seed Oil  
S/N Characteristic  Requirement  
 Test Method  
I Relative Density ( 20oC/ water at 
20oC )  0.91-0.921  IUPAC 2.101  
Ii Refractive Index (n D 40  1.458 – 1.466  US ISO 6320  
Iii Saponification Value (mg KOH/g 
oil) 189 – 198 US ISO 3657  
Iv Iodine Value (Wij’s)  99 – 119 US ISO 3961 1 
V Colour, units in a 25.4 mm 
Lovibond cell, max  Depends on gossypol content  US ISO 15305   
Vi Unsaponifiable Matter, g/kg, max.   
1.5 US ISO 3596  
viii Acid Value, mg KOH/g, max.   
Virgin 4.0  
Non Virgin 0.6  US ISO 660  
Ix Peroxide Value, meq. Peroxide 
oxygen/kg, max.  10 US ISO  3960  
iv Iron, mg/kg  Virgin 5  
Non Virgin 1.5  US ISO 8294  
v Copper, mg/kg  Virgin 0.4  
Non Virgin 0.1  DUS 170: 2018  
© UNBS 2018  – All rights reserved  3 
  
4.3 Fortification  
Refined Cottonseed oil shall contain Vitamin A , Vitamin D, Vitamin E and their esters, and other vitamins in 
accordance  with US EAS 769 . 
5 Food additives   
Only the food additives per mitted in US 45 may be used . 
6 Contaminants  
6.1 Pesticide residues  
The maximum levels of pesticide residues in cottonseed oil shall conform to the internationally accepted levels 
recommended by Codex Alimentarius Commission.  
6.2 Heavy metals  
The level of contaminants in Edible Cottonseed oil shall comply with those maximum pesticide residue limits 
established by CODEX Alimentarius commission for similar commodities . 
Table 2 —Heavy metals limits for Edible Cotton seed oil  
S/N Contaminants  Maximum Level  Test Method  
i Lead, mg/kg  0.1 US SO 12193  
ii Arsenic, mg/kg  0.1 US ISO 2590  
 
7 Hygiene  
Edible Cotton  seed oil shall be produced, processed, handled and stored in accordance with EAS 39.  
8 Weights and measures  
Edible cotton seed oil shall be packaged in ac cordance with the Weights and measures regulations of the 
destination country  
5 Packaging  borrowing  
Edible Cotton seed oil shall be packaged in food grade containers which will safeguard the hygienic, 
nutritional, and organoleptic qualities of the product .. 
6 Labelling  borrowing  
In addition to the requirements in US EAS 38, each package shall be legibly and indelibly labelled with the 
following:  
a) name of the product shall be “Cottonseed oil ”; 
b) brand name/trade name ;  
4 © UNBS 2018  – All rights reserved  
 c) name and physical address of the manufac turer; 
d) lot identification ;  
e) batch/code number ; 
f) net weight in metric units ; 
g) date of manufacture;  
h) country of origin;  
i) storage conditions;  and  
j) expiry dat e/best before date  
7 Sampling  borrowing  
Sampling of  rice flour shall be done in accordance with and prepa red in accordance to US ISO 661  
 DUS 170: 2018  
© UNBS 2018  – All rights reserved  5 
 Bibliography  
[1] CODEX STAN. 22, 1981 (Rev 1, 1989), Codex standard for edible cottonseed oil.  
[2]. Technical Specifications for the manufacture of: Fortified Refined Cottonseed Oil -COTTONSEED OIL  
[3] US 170: 2000, Cottons eed oil   
 DUS 170: 2018  
© UNBS 2018  – All rights reserved  7 
 Certification marking  
Products that conform to Uganda standards may be marked with Uganda National Bureau of Standards 
(UNBS) Certification Mark shown in the figure below.  
The use of the UNBS Certification Mark is governed by the Standards Act , and the Regulations made 
thereunder. This mark can be used only by those licensed under the certification mark scheme operated by 
the Uganda National Bureau of Standards and in conjunction with the relevant Uganda Standard. The 
presence of this mark on a  product or in relation to a product is an assurance that the goods comply with the 
requirements of that standard under a system of supervision, control and testing in accordance with the 
certification mark scheme of the Uganda National Bureau of Standards . UNBS marked products are 
continually checked by UNBS for conformity to that standard.  
Further particulars of the terms and conditions of licensing may be obtained from the Director, Uganda 
National Bureau of Standards.  
 
 DUS 170: 20148    
 
ICS 67.200.10  
Price based on nn pages  
© UNBS 2018 – All rights reserved