ANNEX I 
 
Identif
ication 
numbe
r of 
the 
additiv
e Name of the 
holder of 
authorisa-
tion Additive Composition, chemical 
formula, description, 
analytical method. Species 
or 
category 
of 
animal Maxi-
mum 
age Minimu
m 
content Maxim
um 
content Minimu
m 
content Maxi
mum 
conten
t 
Other provisions End of 
period of 
authori-
sation mg 
guanidinoacetic 
acid/kg of 
complete feed with 
a moisture content 
of 12 % mg 
guanidinoacetic 
acid/l of water 
for drinking 
Category: Zootechnical additives. Functional group: other zootechnical additives (improvement of performance parameters)  
4d372 Alzchem 
Trostberg 
GmbH Guanidin
oacetic 
acid Additive composition: 
Guanidinoacetic acid 98% 
on a dry matter basis 
 
Solid form 
 
Characterisation of the 
active substance: 
Guanidinoacetic acid 
produced by chemical 
synthesis 
 
Chemical formula: 
C3H7N3O2 
 
CAS number: 352-97-6 
 
Purity: 98% 
 
Impurities  : Chickens 
reared 
for 
laying 
and for 
breeding - 600 1200 300 600 1.  The moisture 
content shall be 
indicated on the 
labelling of the 
additive. 
2. The additive 
may be used via 
water for 
drinking.  
3. In the directions 
for use of the 
additive and 
premixtures, the 
storage 
conditions, the 
stability to heat 
treatment and 
the stability in 
water for [10 years 
from the 
date of 
entry into 
force of this 
Regulation. 
To be 
completed 
by the 
Service 
responsible 
for the 
publication
]  — maximum cyanamide 
content 0,03 % ; 
 — maximum dicyandiamide 
content 0,5 %. 
 
Analytical method1: 
For the determination of 
guanidinoacetic acid in the 
compound feed and water: 
ion chromatography coupled 
with ultraviolet detection 
(IC-UV). drinking shall be 
indicated. 
4. When using the 
additive, 
attention shall be 
paid to the 
supply with 
vitamin B 12 and 
methyl donors 
other than 
methionine in 
the diet of the 
animal. 
5. Simultaneous 
use of this 
additive in water 
for drinking and 
in feed is not 
permitted. 
 
  
 
1 Details of the analytical methods are available at the following address of the Reference Laboratory: https://joint-research-centre.ec.europa.eu/eurl-fa-eurl-feed-
additives/eurl-fa-authorisation/eurl-fa-evaluation-reports_en 
  
Identif
ication 
numbe
r of 
the 
additiv
e Name of the 
holder of 
authorisa-
tion Additive Composition, chemical 
formula, description, 
analytical method. Species 
or 
category 
of 
animal Maxi-
mum 
age Minimu
m 
content Maxim
um 
content Minimu
m 
content Maxi
mum 
conten
t Other provisions End of 
period of 
authori-
sation 
mg 
guanidinoacetic 
acid/kg of 
complete feed with 
a moisture content 
of 12 % mg 
guanidinoacetic 
acid/l of water 
for drinking   
Category: Zootechnical additives. Functional group: other zootechnical additives (improvement of performance parameters)  
4d372i Alzchem 
Trostberg 
GmbH Guanidin
oacetic 
acid Additive composition: 
Preparation containing a 
minimum of 96 % of 
guanidinoacetic acid.  
 
Solid form 
 
Characterisation of the 
active substance: 
 
Guanidinoacetic acid 
produced by chemical 
synthesis 
 
Chemical formula: 
C3H7N3O2 
 
CAS number: 352-97-6 
 
Purity: 98% 
 
Impurities:  Chickens 
reared 
for 
laying 
and for 
breeding - 600 1200 300 600 1.  The moisture 
content shall be 
indicated on the 
labelling of the 
additive. 
2. The additive 
may be used via 
water for 
drinking.  
3. In the directions 
for use of the 
additive and 
premixtures, the 
storage 
conditions, the 
stability to heat 
treatment and 
the stability in 
water for 
drinking shall be 
indicated. [10 years 
from the 
date of 
entry into 
force of this 
Regulation. 
To be 
completed 
by the 
Service 
responsible 
for the 
publication
]  — maximum cyanamide 
content 0,03 % ; 
 — maximum dicyandiamide 
content 0,5 %. 
 
Analytical method2: 
For the determination of 
guanidinoacetic acid in the 
compound feed and water: 
ion chromatography coupled 
with ultraviolet detection 
(IC-UV). 4. When using the 
additive, 
attention shall be 
paid to the 
supply with 
vitamin B 12 and 
methyl donors 
other than 
methionine in 
the diet of the 
animal. 
5. Simultaneous 
use of this 
additive in water 
for drinking and 
in feed is not 
permitted. 
 
  
 
2 Details of the analytical methods are available at the following address of the Reference Laboratory: https://joint-research-centre.ec.europa.eu/eurl-fa-eurl-feed-
additives/eurl-fa-authorisation/eurl-fa-evaluation-reports_en 
 Identif
ication 
numbe
r of 
the 
additiv
e Name of the 
holder of 
authorisa-
tion Additive Composition, chemical 
formula, description, 
analytical method. Species 
or 
category 
of 
animal Maximum age  
Minimum 
content  
Maximum 
content Other provisions End of 
period of 
authori-
sation 
mg guanidinoacetic acid/l of 
water for drinking   
Category: Zootechnical additives. Functional group: other zootechnical additives (improvement of performance parameters)  
4d372 Alzchem 
Trostberg 
GmbH Guanidin
oacetic 
acid Additive composition: 
Guanidinoacetic acid 98% 
on a dry matter basis 
 
Solid form 
 
Characterisation of the 
active substance: 
 
Guanidinoacetic acid 
produced by chemical 
synthesis 
 
Chemical formula: 
C3H7N3O2 
 
CAS number: 352-97-6 
 
Purity: 98% 
 
Impurities : 
 — maximum cyanamide 
content 0,03 % ; 
 — maximum dicyandiamide 
content 0,5 %. Chickens 
for 
fattening  - 300 600 1.  The moisture 
content shall be 
indicated on the 
labelling of the 
additive. 
2. The additive 
may be used via 
water for 
drinking.  
3. In the directions 
for use of the 
additive and 
premixtures, the 
storage 
conditions, the 
stability to heat 
treatment and 
the stability in 
water for 
drinking shall be 
indicated. 
4. When using the 
additive, 
attention shall be [10 years 
from the 
date of 
entry into 
force of this 
Regulation. 
To be 
completed 
by the 
Service 
responsible 
for the 
publication
]     
     
Analytical method3: 
For the determination of 
guanidinoacetic acid in 
water: ion chromatography 
coupled with ultraviolet 
detection (IC-UV). paid to the 
supply with 
vitamin B 12 and 
methyl donors 
other than 
methionine in 
the diet of the 
animal. 
5. Simultaneous 
use of this 
additive in water 
for drinking and 
in feed is not 
permitted. 
  
 
3 Details of the analytical methods are available at the following address of the Reference Laboratory: https://joint-research-centre.ec.europa.eu/eurl-fa-eurl-feed-
additives/eurl-fa-authorisation/eurl-fa-evaluation-reports_en 
 Identif
ication 
numbe
r of 
the 
additiv
e Name of the 
holder of 
authorisa-
tion Additive Composition, chemical 
formula, description, 
analytical method. Species 
or 
category 
of 
animal Maximum age  
Minimum 
content  
Maximum 
content Other provisions End of 
period of 
authori-
sation 
mg guanidinoacetic acid/l of 
water for drinking   
Category: Zootechnical additives. Functional group: other zootechnical additives (improvement of performance parameters)  
4d372i Alzchem 
Trostberg 
GmbH Guanidin
oacetic 
acid Additive composition: 
Preparation containing a 
minimum of 96 % of 
guanidinoacetic acid.  
 
Solid form 
 
Characterisation of the 
active substance: 
 
Guanidinoacetic acid 
produced by chemical 
synthesis 
 
Chemical formula: 
C3H7N3O2 
 
CAS number: 352-97-6 
 
Purity: 98% 
 
Impurities : 
 — maximum cyanamide 
content 0,03 % ; 
 — maximum dicyandiamide 
content 0,5 %. Chickens 
for 
fattening  - 300 600 1.  The moisture 
content shall be 
indicated on the 
labelling of the 
additive. 
2. The additive 
may be used via 
water for 
drinking.  
3. In the directions 
for use of the 
additive and 
premixtures, the 
storage 
conditions, the 
stability to heat 
treatment and 
the stability in 
water for 
drinking shall be 
indicated. 
4. When using the 
additive, 
attention shall be 
paid to the [10 years 
from the 
date of 
entry into 
force of this 
Regulation. 
To be 
completed 
by the 
Service 
responsible 
for the 
publication
]     
     
Analytical method4: 
For the determination of 
guanidinoacetic acid in 
water: ion chromatography 
coupled with ultraviolet 
detection (IC-UV). supply with 
vitamin B 12 and 
methyl donors 
other than 
methionine in 
the diet of the 
animal. 
5. Simultaneous 
use of this 
additive in water 
for drinking and 
in feed is not 
permitted. 
 
 
  
 
4 Details of the analytical methods are available at the following address of the Reference Laboratory: https://joint-research-centre.ec.europa.eu/eurl-fa-eurl-feed-
additives/eurl-fa-authorisation/eurl-fa-evaluation-reports_en 
  
ANNEX II 
“ 
Identifi-
cation 
number 
of the 
additive Name of 
the holder 
of 
authorisa-
tion Additive Composition, chemical formula, 
description, analytical method. Species or 
category 
of animal Maxi-
mum 
age Minimum 
content Maximu
m 
content Other provisions End of 
period of 
authori-
sation 
mg guanidinoacetic 
acid/kg of complete 
feed with  
a moisture content of 
12 %   
Category: Zootechnical additives. Functional group: other zootechnical additives (improvement of performance parameters)   
4d372 Alzchem 
Trostberg 
GmbH Guanidino
acetic acid Additive composition : 
Guanidinoacetic acid 98% on dry 
matter basis 
 
Solid form 
 
Characterisation of the active 
substance: 
Guanidinoacetic acid produced by 
chemical synthesis 
Chemical formula: C 3H7N3O2 
CAS number: 352-97-6 
Purity: 98% 
 
Impurities: 
 — maximum cyanamide content 
0,03 %; 
 — maximum dicyandiamide 
content 0,5 %. Chickens 
for 
fattening  
 
 - 600 1200 
 
 1. The moisture content shall be 
indicated on the labelling of 
the additive. 
2. In the directions for use of 
the additive and premixtures, 
the storage conditions and the 
stability to heat treatment 
shall be indicated. 
3. When using the additive, 
attention shall be paid to the 
supply with vitamin B 12 and 
methyl donors other than 
methionine in the diet of the 
animal. 
4. Simultaneous use of this 
additive in water for drinking 
and in feed is not permitted. 25 October 
2026 
 
Weaned 
piglets 600 1200 
Pigs for 
fattening  600 1200  
Analytical method5: 
For the determination of 
guanidinoacetic acid in the 
compound feed: ion 
chromatography coupled with 
ultraviolet detection (IC -UV).  
 
 
  
 
5 Details of the analytical methods are available at the following address of the Reference Laboratory: https://joint-research-centre.ec.europa.eu/eurl-fa-eurl-feed-
additives/eurl-fa-authorisation/eurl-fa-evaluation-reports_en 
 Identifi-
cation 
number 
of the 
additive Name of 
the holder 
of 
authorisa-
tion Additive Composition, chemical formula, 
description, analytical method. Species or 
category 
of animal Maxi-
mum 
age Minimum 
content Maximu
m 
content Other provisions End of 
period of 
authori-
sation 
mg guanidinoacetic 
acid/kg  
of complete feed with  
a moisture content of 
12 %   
Category: Zootechnical additives. Functional group: other zootechnical additives (improvement of performance parameters)   
4d372i Alzchem 
Trostberg 
GmbH Guanidino
acetic acid Additive composition : 
Preparation containing a minimum 
of 96 % of guanidinoacetic acid. 
 
Solid form 
 
Characterisation of the active 
substance: 
Guanidinoacetic acid produced by 
chemical synthesis 
Chemical formula: C 3H7N3O2 
CAS number: 352-97-6 
Purity: 98% 
 
Impurities: 
 — maximum cyanamide content 
0,03 %; 
 — maximum dicyandiamide 
content 0,5 %. 
 
Analytical method6: 
For the determination of Chickens 
for  
fattening  
 
 - 600 1200 
 
 1. The moisture content shall be 
indicated on the labelling of 
the additive. 
2. In the directions for use of 
the additive and premixtures, 
the storage conditions and the 
stability to heat treatment 
shall be indicated. 
3. When using the additive, 
attention shall be paid to the 
supply with vitamin B 12 and 
methyl donors other than 
methionine in the diet of the 
animal. 
4. Simultaneous use of this 
additive in water for drinking 
and in feed is not permitted. 
 25 October 
2026 
 
Weaned 
piglets 600 1200 
Pigs for 
fattening  600 1200 
 
6 Details of the analytical methods are available at the following address of the Reference Laboratory: https://joint-research-centre.ec.europa.eu/eurl-fa-eurl-feed-
additives/eurl-fa-authorisation/eurl-fa-evaluation-reports_en 
 guanidinoacetic acid in the 
compound feed: ion 
chromatography coupled with 
ultraviolet detection (IC-UV). 
”