[Attachment 1]
Additional requirements related to T omato Brown Rugose Fruit Virus(T oBRFV)
 □ Products covered
  ○ Seeds for sowing and Seedlings(such as nursery·root stocks, cuttings and scions) 
of (1) Capsicum  spp. (2) Solanum lycopersicum  syn. Lycopersicon esculentum
 □ Regions or Countries covered (35): where ToBRFV has distributed
  ○ Asia(10): China(Shandong, Yunnan), Iran, Israel, Jordan, Lebanon, Palestine, 
Saudi Arabia, Syria, Türkiye, Uzbekistan
  ○ Europe(22): Albania, Austria, Belgium, Bulgaria, Cyprus, Czech Republic, 
Finland, France, Germany, Greece, Hungary, Italy, Malta, Netherland, Norway, 
Poland, Portugal, Slovak Republic, Slovenia, Spain, Switzerland, United 
Kingdom(England)
  ○ America(3): Canada, Mexico, United States(States of Florida)
 □ Requirement of the imports from ToBRFV distributed regions or countries
  ○ (Seeds for Sowing)  Exports will need to satisfy the conditions of either (1) or (2):
    (1) By certifying that the exports has been produced at the regions free of 
Tomato Brown Rugose Fruit Virus(ToBRFV) in the distributed country. 
Each consignment must be accompanied by Phytosanitary Certificate 
endorsed with the following additional declaration:
 “ The [insert botanical name(s) (Genus species)] seeds have been sourced 
from a ‘pest free area’(or ‘pest free place of production’) free from 
ToBRFV, established and managed according to ISPM 4(or ISPM 10.)”
    (2) By certifying that the exports are free from Tomato Brown Rugose Fruit 
Virus(ToBRFV) through PCR test before export.
- Quantity of seeds for PCR test: Over 4,600 seeds are needed. Sub-sample 
should be under 400 seeds. In case of small seed lots(under 46,000 seeds), 
the test sample should be at least 10% of the lots.
Each consignment must be accompanied by Phytosanitary Certificate endorsed 
with the following additional declaration.“ The seeds were tested by PCR before export and found free from 
ToBRFV(Tomato brown rugose fruit virus)”
Or each consignment should be accompanied by the Phytosanitary Certificate 
and a separate “PCR Inspection Certificate (see attachment 2)
  ○ (Seedlings such as nursery·root stocks, cuttings and scions (except seeds for sowing))  
Exports will need to satisfy the conditions of either (1) or (2):
    (1) By certifying that the exports has been produced at the regions free from 
Tomato Brown Rugose Fruit Virus(ToBRFV) in the distributed country. 
Each consignment must be accompanied by the Phytosanitary Certificate 
endorsed with the following additional declaration:
 “ The [insert botanical name(s) (Genus species)] seedlings have been 
sourced from a ‘pest free area’(or ‘pest free place of production’) free from 
ToBRFV, established and managed according to ISPM 4(or ISPM 10.)”
    (2) By certifying that the exports were produced with seeds uninfected by 
ToBRFV(through PCR test), and has been found free from ToBRFV during 
active growing period through visual inspection and PCR test.
Each consignment must be accompanied by Phytosanitary Certificate endorsed 
with following additional declaration.
“ The plants[botanical name(s) (Genus species)] were produced from seeds 
uninfected with ToBRFV(Tomato brown rugose fruit virus), and free from 
ToBRFV as a result of visual inspection and PCR test”
Or each consignment should be accompanied by the Phytosanitary Certificate 
and a separate “PCR Inspection Certificate (see attachment 2)”
    PCR test results from public testing agencies and labs authorized by the NPPO 
(educational organizations, commercial testing or seed industry labs etc) can be accepted.
* Above PCR test means conventional RT-PCR or real-time RT-PCR test.