ELI: http://data.europa.eu/eli/reg_impl/2024/252/oj 1/4COMMISSION IMPLEMENTING REGUL ATION (EU) 2024/252 
of 16 Januar y 2024
concer ning the renewal of the author isation of a preparation of Lactiplantibacillus plantar um DSM 
23375 as a feed additiv e for all animal species and amending Implementing Regulation (EU) 
No 1065/2012 
(Text with EEA relevance) 
THE EUR OPEAN COMMISSION,
Havi ng regar d to the Treaty on the Functioning of the European Union,
Havi ng rega rd to Regulation (EC) No 1831/2003 of the European Parliament and of the Council of 22 Sept ember 2003 on 
additives for use in animal nutr ition (1), and in particular Article 9(2) thereof,
Whereas:
(1) Regulation (EC) No 1831/2003 provid es for the author isation of additives for use in animal nutr ition and for the 
grounds and procedures for granting and renewing such author isation.
(2) The preparation of Lactiplantibacillus plantar um DSM 23375 (previously taxonomically identif ied as Lactobacillus 
plantar um DSM 23375) was author ised for a period of 10 years as a feed additive for all animal species by 
Commission Implementing Regulation (EU) No 1065/2012 (2).
(3) In accordance with Article 14(1) of Regulation (EC) No 1831/2003, an application was submitted for the renewal of 
the author isation of the preparation of Lactiplantibacillus plantar um DSM 23375 as a feed additive for all animal 
species, requesting the additive to be classif ied in the additive catego ry ‘technological additives ’ and in the functional 
group ‘silag e additives’. That application was accompan ied by the particulars and documents required under 
Article 14(2) of that Regulation.
(4) The European Food Safet y Author ity (‘the Auth ority’) concluded in its opinion of 12 May 2023 (3)that the 
preparation of Lactiplantibacillus plantar um DSM 23375 remains safe for all animal species, the consumers and the 
environment under the conditions of use currently author ised, including the proposed use of a new carrier in the 
formulation of the preparation. It also concluded that the additive is not irritant to skin or eyes but that, owin g to 
its prote inaceous nature, it should be considered a respirator y sensitiser . The Author ity could not conclude on the 
skin sensitisation poten tial of the additive. It also indicate d that there is no need for assessing the efficacy of the 
additive in the context of the renewal of the author isation.
(5) The Reference Laborator y set up by Regulation (EC) No 1831/2003 considered that the conclusions and 
recommendations reach ed in the assessment carried out regar ding the method of analysis of the preparation of 
Lactiplantibacillus plantar um DSM 23375 as a feed additive in the conte xt of the previous author isation are valid and 
applicable for the current application. In accordance with Article 5(4), point (c), of Commission Regulation (EC) 
No 378/2005 (4), an evaluation repor t of the Reference Laboratory is theref ore not required.
(1)OJ L 268, 18.10.2003, p. 29.
(2)Commission Implementing Regulation (EU) No 1065/2012 of 13 November 2012 concer ning the author isation of preparations of 
Lactobacillus plantar um (DSM 23375, CNCM I-3235, DSM 19457, DSM 16565, DSM 16568, LMG 21295, CNCM MA 18/5U, NCIMB 
30094, VTT E-78076, ATCC PTSA-6139, DSM 18112, DSM 18113, DSM 18114, ATCC 55943 and ATCC 55944) as feed additives 
for all animal species (OJ L 314, 14.11.2012, p. 15).
(3)EFSA Journal 2023;21(6):8054.
(4)Commission Regulation (EC) No 378/2005 of 4 March 2005 on detailed rules for the implement ation of Regulation (EC) 
No 1831/2003 of the European Parliament and of the Council as regards the duties and tasks of the Community Reference 
Laborato ry concer ning applications for author isations of feed additives (OJ L 59, 5.3.2005, p. 8).
Offi cial Jour nal  
of the European Union EN 
L series    
2024/252  17.1.2024ELI: http://data.europa.eu/eli/reg_im pl/2024/252/oj 2/4(6) In view of the above, the Commission considers that the preparation of Lactiplantibacillus plantar um DSM 23375 
satisfies the conditions provided for in Article 5 of Regulation (EC) No 1831/2003. According ly, the author isation 
of that additive should be renewed. In addition, the Commission considers that appropr iate protective measures 
should be taken to prevent adverse effects on the health of the users of the additive.
(7) As a consequence of the renewal of the author isation of the preparation of Lactiplantibacillus plantar um DSM 23375 
as a feed additive, Implementing Regulation (EU) No 1065/2012 should be amended according ly.
(8) Since safet y reasons do not require the immediate application of the modifications to the conditions of author isation 
of Lactiplantibacillus plantar um DSM 23375, it is appropr iate to provid e for a transitional period for interested parties 
to prepare themselves to meet the new requirements resulting from the renewal of the author isation.
(9) The measures provid ed for in this Regulation are in accordance with the opinion of the Standing Committ ee on 
Plants, Animals, Food and Feed,
HAS ADOPTED THIS REGUL ATION:
Article 1
Renewal of the author isation
The author isation of the preparation specified in the Annex, belonging to the additive cate gory ‘technological additives’ and 
to the functional group ‘silag e additives’, is renewed subject to the conditions laid down in that Annex.
Article 2
Amendment to Implementing Regulation (EU) No 1065/2012
In the Annex to Implementing Regulation (EU) No 1065/2012, entr y 1k20716 on ‘Lactobacillus plantar um (DSM 23375)’ is 
delet ed.
Article 3
Transitional measures
The preparation specifi ed in the Annex and feed containing it, which are produced and labelled before 6 Febr uary 2025 in 
accordance with the rules applicable before 6 Febr uary 2024 may continue to be placed on the mark et and used until the 
existing stoc ks are exhausted.
Article 4
Entr y into force
This Regulation shall enter into force on the twentieth day followi ng that of its publication in the Official Journal of the 
European Union.OJ L, 17.1.2024 EN  ELI: http://data.europa.eu/eli/reg_impl/2024/252/oj 3/4This Regulation shall be binding in its entirety and directly applicable in all Member States.
Done at Brussels, 16 Januar y 2024 .
For the Commission
The President
Ursula VON DER LEYENOJ L, 17.1.2024 EN  ELI: http://data.europa.eu/eli/reg_im pl/2024/252/oj 4/4ANNEX 
Identification 
number of the 
additiveAdditiveComposition, chemical formula, 
descr iption, analytical methodSpecies or 
categ ory of 
animalMaximum 
ageMinimum 
conten tMaximum 
conten tOther provisi onsEnd of period of 
author isation
CFU/kg of fresh material
Categor y: technological additiv es. Functional group: silage additiv es
1k20716 Lactiplantiba -
cillus 
plantar um 
DSM 23375Additive composition
Preparation of Lactiplantibacillus 
plantar um DSM 23375 containing a 
minimum of 2 × 1010 CFU/g additive
Solid formAll animal 
species— — 1. In the directions for use of the additive and pre­
mixtures, the storag e conditions shall be indi ­
cated .
2. Minimum dose of the additive when used with ­
out combination with other micro-org anisms 
as silage additives: 1×108 CFU/kg fresh mat erial.
3. For users of the additive and premixtures, feed 
business operat ors shall establish operational 
procedures and organisational measures to ad­
dress poten tial risks resulting from their use. 
Where those risks cannot be eliminated by such 
procedures and measures, the additive and pre­
mixtures shall be used with personal skin and 
breathing prot ective equipment.6 Febr uary 2034
Char acter isation of the active substance
Viable cells of Lactiplantibacillus 
plantar um DSM 23375
Analytical method (1)
Enumeration in the feed additive of 
Lactiplantibacillus plantar um DSM 
23375:
— Spread plate method using MRS 
Agar (EN 15787)
Identif ication of Lactiplantibacillus 
plantar um DSM 23375:
— Pulsed Field Gel Electrophoresis 
(PFGE) – CEN/TS 17697 or 
DNA sequencing methods
(1)Details of the analytical methods are available at the following address of the Reference Laborato ry: https://joint-research-c entre.ec.europa.eu/eurl-fa-eurl-f eed-additives/eurl-fa-author isation/eurl-f a-evaluation- 
repor ts_enOJ L, 17.1.2024 EN