COMMISSION IMPLEMENTING REGUL ATION (EU) 2023/1709 
of 7 September 2023
concer ning the author isation of a preparation of Lentilactobacillus diolivo rans DSM 33625 as a feed 
additiv e for all animal species 
(Text with EEA relevance) 
THE EUR OPEAN COMMISSION,
Havi ng regar d to the Treaty on the Functioning of the European Union,
Havi ng rega rd to Regulation (EC) No 1831/2003 of the European Parliament and of the Council of 22 Sept ember 2003 on 
additives for use in animal nutr ition (1), and in particular Article 9(2) thereof,
Whereas:
(1) Regulation (EC) No 1831/2003 provid es for the author isation of additives for use in animal nutr ition and for the 
grounds and procedures for granting such an author isation.
(2) In accordance with Article 7 of Regulation (EC) No 1831/2003, an application was submitted for the author isation 
of a preparation of Lentilactobacillus diolivo rans DSM 33625 (previously taxonomically identif ied as Lactobacillus 
diolivo rans DSM 33625). That application was accompanied by the particulars and documents required under 
Article 7(3) of Regulation (EC) No 1831/2003.
(3) The application concer ns the author isation of the preparation of Lentilactobacillus diolivo rans DSM 33625 as a feed 
additive for all animal species, requesting that additive to be classif ied in the catego ry ‘technological additives ’ and in 
the functional group ‘silag e additives ’.
(4) The European Food Safety Author ity (‘the Author ity’) concluded in its opinion of 17 Januar y 2023 (2)that, under the 
proposed conditions of use, the preparation of Lentilactobacillus diolivo rans DSM 33625 is safe for the targe t species, 
consumers and the environment. It also concluded that the preparation is not a skin or an eye irritant, that in the 
absence of data, no conclusion can be drawn on the skin sensitisation of the additive and that, given the 
prote inaceous nature of the active agent, the additive should be considered a respirator y sensitiser . The Author ity 
further concluded that Lentilactobacillus dioliv orans DSM 33625 at a minimum concentration of 1 × 108 CFU/kg 
forag e may exte nd the aerobic stability of silage prepared from easy and moderately difficult to ensile forag e 
mater ial with a dry matte r range of 32–65 %. The Authority also verified the repor t on the method of analysis of 
the feed additive in feed submitte d by the Reference Laboratory set up by Regulation (EC) No 1831/2003.
(5) In view of the above, the Commission considers that the preparation of Lentilactobacillus dioliv orans DSM 33625 
satisfies the conditions provided for in Article 5 of Regulation (EC) No 1831/2003. According ly, the use of that 
preparation should be author ised. In addition, the Commission considers that appropr iate prote ctive measures 
should be taken to prevent adverse effects on the health of the users of the additive.
(6) The measures provid ed for in this Regulation are in accordance with the opinion of the Standing Committ ee on 
Plants, Animals, Food and Feed,
HAS ADOPTED THIS REGUL ATION:
Article 1
The preparation specifi ed in the Annex, belonging to the additive catego ry ‘techn ological additives’ and to the functional 
group ‘silag e additives’, is author ised as an additive in animal nutr ition, subject to the conditions laid down in that Annex.
(1)OJ L 268, 18.10.2003, p. 29.
(2)EFSA Journal 2023;21(2):7820.EN Offi cial Jour nal of the European Union L 221/34 8.9.2023  Article 2
This Regulation shall enter into force on the twentieth day followi ng that of its publication in the Official Journal of the 
European Union.
This Regulation shall be binding in its entirety and directly applicable in all Member States.
Done at Brussels, 7 Sept ember 2023 .
For the Commission
The President
Ursula VON DER LEYENEN Offi cial Jour nal of the European Union 8.9.2023 L 221/35  ANNEX 
Identifica -
tion 
number of 
the additiveAdditiveCompo sition, chem ical formula, 
descr iption, analytical methodSpecies or 
categor y of 
animalMaximum 
ageMinimum 
contentMaximum 
content
Other provisionsEnd of period of 
author isationCFU/kg of fresh 
material
Categor y: technological additiv es. Functional group: silage additiv es
1k21901 Lentilactobacillus 
diolivo rans DSM 
33625Additive composition
Preparation of Lentilactobacillus 
diolivo rans DSM 33625 containing a 
minimum of 2 × 1011 CFU/g additive
Solid formAll animal 
species- - 1.In the directions for use of the ad­
ditive and premixtures, the storage 
conditions shall be indicate d.
2.Minimum dose of the additive when 
used without combination with 
other micro-org anisms as silage ad­
ditives: 1 × 108 CFU/kg of easy and 
moderately difficult to ensile fresh 
mate rial(2).
3.For users of the additive and pre­
mixtures, feed business operat ors 
shall establish operational proce ­
dures and organisational measures 
to address poten tial risks resulting 
from their use. Where those risks 
cannot be eliminated by such pro­
cedures and measures, the additive 
and premixtures shall be used with 
personal skin and breathing prote c­
tive equipment.28 Sept ember 2033
Char acter isation of the active substance
Viable cells of Lentilactobacillus 
diolivo rans DSM 33625
Analytical method (1)
Enumeration in the feed additive of 
Lentilactobacillus diolivo rans DSM 33625:
— Spread plate method on MRS agar 
(EN 15787)
Identif ication of Lentilactobacillus 
diolivo rans DSM 33625:
— Pulsed Field Gel Electrophoresis 
(PFGE) or DNA sequencing methods
(1)Details of the analytical methods are available at the following address of the Reference Laborato ry: https://joint-research-c entre.ec.europa.eu/eurl-fa-eurl-f eed-additives/eurl-fa-author isation/eurl-f a-evaluation- 
repor ts_en .
(2)Easy to ensile forage: > 3 % soluble carbohydrat es in fresh material ; moderat ely difficult to ensile forage: 1,5–3,0 % soluble carbohy drates in the fresh mat erial in accordance with Commission Regulation (EC) 
No 429/2008 of 25 Apr il 2008 on detailed rules for the impl ementation of Regulation (EC) No 1831/2003 of the European Parliament and of the Council as regards the preparation and the presentation of 
applications and the assessment and the author isation of feed additives (OJ L 133, 22.5.2008, p. 1).EN Offi cial Jour nal of the European Union L 221/36 8.9.2023