Calcium Carbonate  II 
炭酸カルシウム  Ⅱ 
 
 
Standards for Use 
Permitted for use in grape juice used for wine production and grape wine  only.  
 
Compositional Specifications  
Substance Name  Calcium Carbonate  II 
Molecular F ormula  CaCO3 
Molecular Weight  100.09  
Chemical Name [CAS number]  
Calcium carbonate [471 -34-1, Calcium carbonate]  
Definition  Calcium Carbonate  II consists  mainly of calcium carbonate . It is produced 
by a method through which the resulting product possibly contai ns the calcium double 
salt of L-tartaric and L-malic acids.  
Content   Calcium Carbonate  II, when calculated on the dried basis,  contains 98.0–
102.0% of calcium carbonate (CaCO 3). 
Description   Calcium Carbonate  II occurs as a fine white powder. It is odorless.  
Identification  To 1 g of Calcium Carbonate  II, add 10 mL of water and 7 mL of diluted 
acetic acid (1 in 4). It effervesces and dissolves. When boiled and neutralized with 
ammonia TS, this solution responds to all  the tests for Calcium Salt in the Qualitative 
Tests.  
Purity  
(1) Hydrochloric acid -insoluble substances   Not more than 0.20%.  
Weigh 5.0 g of Calcium Carbonate  II, add 10 mL of water, then gradually add 12 mL 
of hydr ochloric acid dropwise while stirring, and add water to make 200 mL. Filter 
through a filter paper for quantitative analysis (5C), and wash the residue on the filter 
paper with boiling water until the washings are free of chloride. Heat gradually the 
resid ue together with the filter paper to carbonization, incinerate at 450 –550 °C for 3 Attachment  hours or more, and weigh.  
(2) Free alkali   Weigh 3.0 g of Calcium Carbonate  II, add 30 mL of freshly boiled and 
cooled water, shake for 3 minutes, and filter the solution. To  20 mL of the filtrate, add 2 
drops of phenolphthalein TS. A pink color develops, and it disappears when 0.20 mL of 
0.1 mol/L hydrochloric acid is added.  
(3) Lead   Not more than 3 μg/g as Pb (2.0 g, Method 5, Control Solution: Lead 
Standard Solution 6.0 mL , Flame Method).  
Sample Solution   To the specified amount of Calcium Carbonate  II, add 20 mL of 
diluted hydrochloric acid (1 in 4), and boil gently for 15 minutes with a watch glass 
covering it. Allow to cool, add 30 mL of water, and use the resulting solu tion as the 
sample solution. If the sample does not dissolve completely, evaporate it to dryness, and 
add 20 mL of diluted hydrochloric acid (1 in 4) to the residue. Boil gently for 5 minutes 
with a watch glass covering it, allow to cool, and add 30 mL of water.  
In Method 5 of the Lead Limit Test, use 50 mL of a solution of diammonium hydrogen 
citrate (1 in 2) and 1 mL of bromothymol blue TS as the indicator instead of thymol blue 
TS, and then add ammonia solution until the color of the solution changes fr om yellow 
to yellow -green.  
(4) Alkali metals and magnesium   Not more than 1%.  
Weigh 1.0 g of Calcium Carbonate  II, dissolve it by gradually adding 30 mL of diluted 
hydrochloric acid (1 in 10), and let the carbon dioxide out by boiling. Cool, neutralize 
with ammonia TS, add 60 mL of a solution of ammonium oxalate monohydrate (1 in 25), 
and heat on a water bath for 1 hour. After cooling, add water to make 100 mL, and stir 
thoroughly. Centrifuge it, and filter the supernatan t. Measure 50 mL of the filtrate, add 
0.5 mL of sulfuric acid, evaporate to dryness, ignite to constant weig ht at 600 °C, and 
weigh.  
(5) Barium   Not more than 0.030% as Ba.  
Test Solution   Weigh 1.0 g of Calcium Carbonate  II, dissolve it in 8 mL of diluted  
hydrochloric acid (1 in 4), and add water to make 20 mL.  
Procedure   Add 2 g of sodium acetate trihydrate, 1 mL of diluted acetic acid (1 in 20),  
and 0.5 mL of potassium chromate solution (1 in 20) to the test solution, and allow to  
stand for 15 minutes. The solution is not more turbid th an a control solution prepared  
as follows: To 0.30 mL of Barium Standard Solution, add water to make 20 mL, and then  
treat in the same manner as  for the test solution.  
(6) Arsenic   Not more than 3 μg/g as As (0.50 g, Standard Color: Arsenic Standard  
Soluti on 3.0 mL, Apparatus B).  
Test Solution   Weigh the specified amount of Calcium Carbonate  II, moisten with 1 
mL of water, and dissolve it in 4 mL of diluted hydrochloric acid (1 in 4).  Loss on Drying   Not more than 2.0% (200 °C, 4 hours).  
Assay   Weigh accurately about 2 g of Calcium Carbonate  II, and add gradually exactly 
50 mL of 1 mol/L hydrochloric acid. Heat the con tainer containing the solution in a water 
bath for abou t 10 minutes. After cooling, titrate the excess hydrochloric acid  with 1mol/L 
sodium hydroxide (indicator: 4 –5 drops of methyl red TS). The endpoint is when the color 
of the solution changes from red to yellow. Calculate on the dried basis.  
Each mL of 1 mol/L hydrochloric acid = 50.04 mg of CaCO 3