COMMISSION IMPLEMENTING REGUL ATION (EU) 2022/385 
of 7 March 2022
correcting Implementing Regulation (EU) 2021/421 concer ning the author isation of tincture derived 
from Artemisia vulg aris L. (mugw ort tincture) as a feed additiv e for all animal species, Implementing 
Regulation (EU) 2021/485 concer ning the author isation as feed additiv es of ginger essential oil from 
Zingiber officinale Roscoe for all animal species, ginger oleoresin from Zingiber officinale Roscoe for 
chickens for fattening, laying hens, turke ys for fattening, piglets, pigs for fattening, sows, dair y cows, 
veal calv es (milk replacers), cattle for fattening, sheep, goats, horses, rabbits, fish and pets and ginger 
tincture from Zingiber officinale Roscoe for horses and dogs and Implementing Regulation (EU) 
2021/551 concer ning the author isation of turmer ic extract, turmer ic oil, turmer ic oleoresin from 
Cur cuma long a L. rhizome as feed additiv es for all animal species and turmer ic tincture from 
Cur cuma long a L. rhizome as a feed additiv e for horses and dogs 
(Text with EEA relevance) 
THE EUR OPEAN COMMISSION,
Havi ng regar d to the Treaty on the Functioning of the European Union,
Havi ng rega rd to Regulation (EC) No 1831/2003 of the European Parliament and of the Council of 22 Sept ember 2003 on 
additives for use in animal nutr ition (1), and in particular Article 9(2) thereof,
Whereas:
(1) The use of tincture derived from Artemisia vulgaris L. (mugwor t tincture) as a feed additive for all animal species was 
author ised by Commission Implementing Regulation (EU) 2021/421 (2)for a 10-year period.
(2) The use of ginger essential oil from Zingiber officinale Roscoe as a feed additive for all animal species, ginger oleoresin 
from Zingiber officinale Roscoe as a feed additive for chick ens for fatteni ng, laying hens, turke ys for fattening , piglets, 
pigs for fattening , sows, dair y cows, veal calves (milk replacers), cattle for fattening, sheep, goats, horses, rabbits, fish 
and pets and ginge r tincture from Zingiber officinale Roscoe as a feed additive for horses and dogs was author ised by 
Commission Implementing Regulation (EU) 2021/485 (3)for a 10-y ear period.
(3) The use of turmer ic extract, turmer ic oil, turmer ic oleoresin from Curcuma long a L. rhizome as feed additives for all 
animal species and turmer ic tincture from Curcuma long a L. rhizome as a feed additive for horses and dogs was 
author ised by Commission Implementing Regulation (EU) 2021/551 (4)for a 10-year period.
(4) In the Annex es to Implementing Regulations (EU) 2021/421, (EU) 2021/485 and (EU) 2021/551, an incor rect 
provision for the labelling of the recommended maximum conte nt of the active substances in premixtures was 
inser ted in the column ‘Other provi sions ’. That provision should only apply to the additives themselves.
(1)OJ L 268, 18.10.2003, p. 29.
(2)Commission Implementing Regulation (EU) 2021/421 of 9 March 2021 concer ning the author isation of tincture derived from 
Artemisia vulgaris L. (mugw ort tincture) as a feed additive for all animal species (OJ L 83, 10.3.2021, p. 21).
(3)Commission Implementing Regulation (EU) 2021/485 of 22 March 2021 concer ning the author isation as feed additives of ginger 
essential oil from Zingiber officinale Roscoe for all animal species, ginger oleoresin from Zingiber officinale Roscoe for chic kens for 
fattening, laying hens, turke ys for fattening, piglets, pigs for fattening, sows, dair y cows, veal calves (milk replacers), cattle for fattening, 
sheep, goats, horses, rabbits, fish and pets and ginger tincture from Zingiber officinale Roscoe for horses and dogs (OJ L 100, 23.3.2021, 
p. 3).
(4)Commission Implementing Regulation (EU) 2021/551 of 30 March 2021 concer ning the author isation of turmer ic extract, turmer ic 
oil, turmer ic oleoresin from Curcuma long a L. rhizome as feed additives for all animal species and turmer ic tincture from Curcuma long a 
L. rhizome as a feed additive for horses and dogs (OJ L 111, 31.3.2021, p. 3).EN Offi cial Jour nal of the European Union 8.3.2022 L 78/21  (5) Implementing Regulations (EU) 2021/421, (EU) 2021/485 and (EU) 2021/551 should theref ore be corrected 
according ly. For the sake of clarity, it is appropr iate to replace the whole Annex to those Implementing Regulations.
(6) In order to allow feed business operat ors to adapt the labelling of the additives and feed containing them to the 
corrected term s of author isation, a transitional period should be provided for as regar ds the placing on the mark et 
of those products.
(7) In order to preser ve the legitimate expectations of the inter ested parties in relation to the term s of the author isation 
of these additives, this Regulation should enter into force as a matte r of urgency .
(8) The measures provid ed for in this Regulation are in accordance with the opinion of the Standing Committ ee on 
Plants, Animals, Food and Feed,
HAS ADOPTED THIS REGUL ATION:
Article 1
The Annex to Implementing Regulation (EU) 2021/421 is replaced by Annex I to this Regulation.
Article 2
The Annex to Implementing Regulation (EU) 2021/485 is replaced by Annex II to this Regulation.
Article 3
The Annex to Implementing Regulation (EU) 2021/551 is replaced by Annex III to this Regulation.
Article 4
The substances specifi ed in Annexes I, II and III and premixtures containing those substances, which are produced and 
labelled before 9 Sept ember 2022 in accordance with the rules applicable before 9 March 2022 may continue to be placed 
on the mark et until the existing stoc ks are exhausted.
Article 5
This Regulation shall enter into force on the day following that of its publication in the Official Journal of the European Union.
This Regulation shall be binding in its entirety and directly applicable in all Member States.
Done at Brussels, 7 March 2022 .
For the Commission
The President
Ursula VON DER LEYENEN Offi cial Jour nal of the European Union L 78/22 8.3.2022  ANNEX I 
Identifi -
cation 
number 
of the 
additiveName of 
the holder 
of 
author isa-
tionAdditiveComposition, chemical formula, descr iption, 
analytical method.Species or 
category of 
animalMaximum 
ageMinimum 
contentMaximum 
conten t
Other provisionsEnd of period 
of 
author isationmg active substance/kg 
of complet e feed with a 
moisture conten t of 
12 %
Categor y: Sensor y additiv es
Functional group: Flavour ing compounds
2b72-t - Mugwor t 
tinctureAdditive composition
Tincture produced from the fragmented 
aerial parts of Artemisia vulgaris L.
Char acter isation of the activ e substance
Tincture produced from the fragmented 
aerial parts of Artemisia vulgaris L by extended 
extraction with a water /ethanol mixture as 
defined by the Council of Europe (1).
The specifications of the active substance are:
Dry matte r: 1,4-1,9 %
Ash: 0,2-0,5 %
Org anic fraction: 1,13-1,65 %, of which
— Total polyphenols:0,05-0,2 %
— Phenolic acids 0,02-0,11 %
— Chlorog enic acid: 0,0028-0,0136 %
— α- and β-thujone: < 0,005 %
— 1,8-cineole: 0,005 %
— Solvent (ethanol): 98,1-98,6 %All animal 
species- - - 1.The additive shall be incor porat ed into 
the feed in the form of a premixture.
2.In the directions for use of the additive 
and premixtures, the stora ge condi ­
tions and stability to heat treatment 
shall be indicate d.
3.On the label of the additive the follo w­
ing shall be indicate d:
‘Recommended maximum cont ent of 
the active substance per kg of compl ete 
feedingstuff with a moisture conte nt of 
12 %: 400 mg/kg’.
4.The functional group, the identif ica­
tion number , the name and the added 
amount of the active substance shall be 
indicated on the label of the premixture 
where the use level on the label of the 
premixture would result in exceed ing 
the level referred to in point 3.
5.For users of the additive and premix ­
tures, feed business operat ors shall es­
tablish operational procedures and or­
gani sational measures to address 
poten tial risks by inhalation, dermal 30.3.2031EN Offi cial Jour nal of the European Union 8.3.2022 L 78/23  CoE number: 72
Liquid form
Analytical method (2)
For the character isation of the feed additive 
(Mugw ort tincture):
—grav imetr ic method for the deter mina ­
tion of loss on drying and the ash conte nt
— spectrophotom etric method for the 
determination of total polyphenols con­
tent
—high performa nce thin layer chromat o­
graph y (HPTLC) method for the determi­
nation of total phenolic acids, chloro­
genic acid, alpha- and beta- thujones 
and eucalypt ol.contact or eye contact. Where those 
risks cannot be eliminate d or reduced 
to a minimum by such procedures and 
measures, the additive and premixtures 
shall be used with personal prot ective 
equipment, including breathing pro­
tectio n, safety glasses and gloves.
(1)Natural sources of flavourings – Repor t No 2 (2007).
(2)Details of the analytical methods are avail able at the follo wing address of the Reference Laboratory : https://ec.europa.eu/jrc/en/eurl/feed-additives/evaluation-repor tsEN Offi cial Jour nal of the European Union L 78/24 8.3.2022  ANNEX II 
Identifi -
cation 
number 
of the 
additiveName of 
the holder 
of 
author isa-
tionAdditiveComposition, chemical formula, descr iption, 
analytical method.Species or 
category of 
animalMaximum 
ageMinimum 
contentMaximum 
conten t
Other provisionsEnd of period 
of 
author isationmg active substance/kg 
of complet e feed with a 
moisture conten t of 
12 %
Categor y: Sensor y additiv es
Functional group: Flavour ing compounds
2b489- -
eo- Ginger 
essential oilAdditive composition
Essential oil derived by steam distillation 
from the dried rhizomes of Zingiber officinale 
Roscoe.
Char acter isation of the activ e substance
Essential oil derived by steam distillation 
from the dried rhizomes of Zingiber officinale 
Roscoe as defined by the Council of 
Europe (1).
— α-zingiberene: 29-40 %
— β-sesquiphellandrene: 8-14 %
— ar-curcumene: 5-12 %
— α-farnesene: 4-10 %
— camp hene: 2-10 %
— β-bisabolene: 2-9 %
CAS number : 8007-08-7
Einecs number: 283-634-2
FEMA number : 2522
CoE number: 489
Liquid form
Analytical method (2)All animal 
species- - - 1.The additive shall be incor porat ed into 
the feed in the form of a premixture.
2.In the directions for use of the additive 
and premixtures, the stora ge condi ­
tions and stability to heat treatment 
shall be indicate d.
3.On the label of the additive the follo w­
ing shall be indicate d:
‘Recommended maximum cont ent of 
the active substance per kg of compl ete 
feedingstuff with a moisture conte nt of 
12 %:
— veal calves (milk replacer): 80 mg;
—other species or cate gories of ani­
mals: 20 mg’.
4.The functional group, the identif ica­
tion number , the name and the added 
amount of the active substance shall be 
indicated on the label of the premixture 
where the use level on the label of the 
premixture would result in exceed ing 
the level referred to in point 3.12.4.2031EN Offi cial Jour nal of the European Union 8.3.2022 L 78/25  For the quantifi cation of alpha-zingiberene, 
beta-sesquiphellandrene and ar-curcumene 
in the feed additive:
— Gas chromat ography coupled to mass 
spectrometr y (GC-MS) (full scan mode) 
using Retenti on Time Lock ing (RTL) 
methodology (or standard substances of 
the phyt ochemical mark ers) with (or 
without) gas chromatograph y coupled 
to flame ionisation detection (GC-FID) 
based on the ISO 11024 standard 
method.5.The mixture of ginge r essential oil with 
other author ised additives obtained 
from Zingiber officinale Roscoe shall 
be not be allowed in feedingstuffs.
6.For users of the additive and premix ­
tures, feed business operat ors shall es­
tablish operational procedures and or­
gani sational measures to address 
poten tial risks by inhalation, dermal 
contact or eye contact. Where those 
risks cannot be eliminate d or reduced 
to a minimum by such procedures and 
measures, the additive and premixtures 
shall be used with personal prot ective 
equipment, including breathing pro­
tectio n, safety glasses and gloves.
(1)Natural sources of flavourings – Repor t No 2 (2007).
(2)Details of the analytical methods are avail able at the follo wing address of the Reference Laboratory : https://ec.europa.eu/jrc/en/eurl/feed-additives/evaluation-repor ts
Identifi -
cation 
number 
of the 
additiveName of 
the holder 
of 
author isa-
tionAdditiveComposition, chemical formula, descr iption, 
analytical method.Species or 
category of 
animalMaximum 
ageMinimum 
contentMaximum 
conten t
Other provisionsEnd of period 
of 
author isationmg active substance/kg 
of complet e feed with a 
moisture conten t of 
12 %
Categor y: Sensor y additiv es
Functional group: Flavour ing compounds
2b489- -
or- Ginger 
oleoresinAdditive composition
Ginge r oleoresin obtained by steam 
distillation and solvent extraction of dried 
rhizomes of Zingiber officinale Roscoe.Chick ens for 
fattening
Laying hens- - - 1.The additive shall be incor porat ed into 
the feed in the form of a premixture.
2.In the directions for use of the additive 
and premixtures, the stora ge condi ­
tions and stability to heat treatment 
shall be indicate d.12.4.2031EN Offi cial Jour nal of the European Union L 78/26 8.3.2022  Char acter isation of the activ e substance
Ginge r oleoresin obtained by steam 
distillation and solvent extraction of dried 
rhizomes of Zingiber officinale Roscoe as 
defined by the Council of Europe (1).
Essential oil: 25-30 (w/w)
Total ginger ols: 0,5-8 % (w/w)
— 6-Ging erol
— 8-Ging erol
— 10-Ging erol
Total shog aols: 3-6 % (w/w)
— 6-Shog aol
— 8-Shog aol
Humidity and volatiles: 25-30 (w/w)
CoE number: 489
Liquid form
Analytical method (2)
For the quantifi cation of the phytochemical 
mark ers: total gingerols and total shog aols in 
the feed additive (ginger oleoresin):
—High performa nce liquid chromat ogra ­
phy (HPL C) with spectrophotometr ic 
(UV) detection – ISO 13685Turkeys for 
fattening
Piglets
Pigs for 
fattening
Sows
Dair y cows
Veal calves 
(milk 
replacers)
Cattle for 
fattening
Sheep and 
goats
Horses
Rabbits
Fish
Pets3.On the label of the additive the follo w­
ing shall be indicate d:
‘Recommended maximum cont ent of 
the active substance per kg of compl ete 
feedingstuff with a moisture conte nt of 
12 % and for milk replacers with a 
moisture conte nt of 5,5 %:
— chick ens for fattening: 5 mg;
— laying hens and rabbits: 7 mg;
— turk eys for fattening: 6 mg;
— piglets: 8 mg;
— pigs for fattening: 10 mg;
— sows: 13 mg;
— dair y cows: 12 mg;
— veal calves (milk replacers): 21 mg;
— cattle for fattening: 19 mg;
— sheep, goats, horses and fish: 20 
mg;
— pets: 1 mg’.
4.The functional group, the identif ica­
tion number , the name and the added 
amount of the active substance shall be 
indicated on the label of the premixture 
where the use level on the label of the 
premixture would result in exceed ing 
the level referred to in point 3.
5.The mixture of ginger oleoresin with 
other author ised additives obtained 
from Zingiber officinale Roscoe shall 
be not be allowed in feedingstuffs.EN Offi cial Jour nal of the European Union 8.3.2022 L 78/27  6.For users of the additive and premix ­
tures, feed business operat ors shall es­
tablish operational procedures and or­
gani sational measures to address 
poten tial risks by inhalation, dermal 
contact or eye contact. Where those 
risks cannot be eliminate d or reduced 
to a minimum by such procedures and 
measures, the additive and premixtures 
shall be used with personal prot ective 
equipment, including breathing pro­
tectio n, safety glasses and gloves.
(1)Natural sources of flavourings – Repor t No 2 (2007).
(2)Details of the analytical methods are avail able at the follo wing address of the Reference Laboratory : https://ec.europa.eu/jrc/en/eurl/feed-additives/evaluation-repor ts
Identifi -
cation 
number 
of the 
additiveName of 
the holder 
of 
author isa-
tionAdditiveComposition, chemical formula, descr iption, 
analytical method.Species or 
category of 
animalMaximum 
ageMinimum 
contentMaximum 
conten t
Other provisionsEnd of period 
of 
author isationmg active substance/kg 
of complet e feed with a 
moisture conten t of 
12 %
Categor y: Sensor y additiv es
Functional group: Flavour ing compounds
2b489-t - Ginger 
tinctureAdditive composition
Ginge r tincture obtained by extraction of 
ground dried rhizomes of Zingiber officinale 
Roscoe using an ethanol/water mixture.
Char acter isation of the activ e substance
Ginge r tincture is obtained by extraction of 
ground dried rhizomes of Zingiber officinale 
Roscoe using an ethanol/water mixture as 
defined by the Council of Europe (1).Horses
Dogs- - - 1.The additive shall be incor porat ed into 
the feed in the form of a premixture.
2.In the directions for use of the additive 
and premixtures, the stora ge condi ­
tions and stability to heat treatment 
shall be indicate d.
3.On the label of the additive the follo w­
ing shall be indicate d:12.4.2031EN Offi cial Jour nal of the European Union L 78/28 8.3.2022  Solvent (ethanol/wat er, 90/10): 97-98 %  
(w/w)
Dry matte r: 2-3 % (w/w)
Total ginger ols: 0,14-0,11 % (w/w)
— 6-Ging erol
— 8-Ging erol
— 10-Ging erol
Total shog aols: 0,043-0,031 % (w/w)
— 6 Shogaol
— 8-Shog aol
Analytical method (2)
For the quantifi cation of the phytochemical 
mark ers: total gingerols and total shog aols in 
the feed additive (ginger tincture):
—High performa nce liquid chromat ogra ­
phy (HPL C) with spectrophotometr ic 
(UV) detection – ISO 13685‘Recommended maximum cont ent of 
the active substance per kg of compl ete 
feedingstuff with a moisture conte nt of 
12 %:
— Horses 1,58 mL
— Dogs 1,81 mL’.
4.The functional group, the identif ica­
tion number , the name and the added 
amount of the active substance shall be 
indicated on the label of the premixture 
where the use level on the label of the 
premixture would result in exceed ing 
the level referred to in point 3.
5.The mixture of ginger tincture with 
other author ised additives obtained 
from Zingiber officinale Roscoe shall 
be not be allowed in feedingstuffs.
6.For users of the additive and premix ­
tures, feed business operat ors shall es­
tablish operational procedures and or­
gani sational measures to address 
poten tial risks by inhalation, dermal 
contact or eye contact. Where those 
risks cannot be eliminate d or reduced 
to a minimum by such procedures and 
measures, the additive and premixtures 
shall be used with personal prot ective 
equipment, including breathing pro­
tectio n, safety glasses and gloves.
(1)Natural sources of flavourings – Repor t No 2 (2007).
(2)Details of the analytical methods are avail able at the follo wing address of the Reference Laboratory : https://ec.europa.eu/jrc/en/eurl/feed-additives/evaluation-repor tsEN Offi cial Jour nal of the European Union 8.3.2022 L 78/29  ANNEX III 
Identifi -
cation 
number 
of the 
additiveName of 
the holder 
of 
author isa-
tionAdditiveComposition, chemical formula, descr iption, 
analytical method.Species or 
category of 
animalMaximum 
ageMinimum 
contentMaximum 
conten t
Other provisionsEnd of period 
of 
author isationmg active substance/kg 
of complet e feed with a 
moisture conten t of 
12 %
Categor y: Sensor y additiv es
Functional group: Flavour ing compounds
2b163- -
eo- Turmer ic 
essential oilAdditive composition
Essential oil derived by steam distillation 
from the dried rhizomes of Curcuma long a L.
Char acter isation of the activ e substance
Essential oil derived by steam distillation 
from the dried rhizomes of Curcuma long a L. 
as defined by the Council of Europe (1):
— ar-Turmerone: 40-60 %
— ß-Turmerone (curlone): 5-15 %
— ar-Curcumene: 3-6 %
— ß-Sesquiphellandrene: 3-6 %
— a-Zingiberene: 1-5 %
— (E)-Atlan tone : 2-4 %
CAS number : 8024-37-1 (2)
Einecs number: 283-882-11
FEMA number : 30851
CoE number: 163
Liquid form
Analytical method (3)All animal 
species- - - 1.The additive shall be incor porat ed into 
the feed in the form of a premixture.
2.In the directions for use of the additive 
and premixtures, the stora ge condi ­
tions and stability to heat treatment 
shall be indicate d.
3.On the label of the additive the follo w­
ing shall be indicate d:
‘Recommended maximum cont ent of 
the active substance per kg of compl ete 
feedingstuff with a moisture conte nt of 
12 % or milk replacers with a moisture 
conte nt of 5,5 %:
— all animal species excep t veal 
calves: 20 mg
— veal calves: 80 mg (milk replacers)’.
4.The functional group, the identif ica­
tion number , the name and the added 
amount of the active substance shall be 
indicated on the label of the premix ­
ture, if the conte nt of the active sub­
stance in complet e feedingstuff speci ­
fied in point 3 is exceeded.20.4.2031EN Offi cial Jour nal of the European Union L 78/30 8.3.2022  For the quantifi cation of the phytochemical 
mark ers: ar-turmerone and beta-tur merone 
in the feed additive (turmer ic oil):
— Gas chromat ography coupled to mass 
spectrometr y (GC-MS) (full scan mode) 
using Retenti on Time Lock ing (RTL) 
methodology (or standard substances of 
the phyt ochemical mark ers) with (or 
without) gas chromatograph y coupled 
to flame ionisation detection (GC-FID) 
based on the ISO 11024 standard method5.The mixture of Turmer ic essential oil 
with other author ised additives ob­
tained from Curcuma long a L. shall be 
not be allowed in feedingstuffs.
6.For users of the additive and premix ­
tures, feed business operat ors shall es­
tablish operational procedures and or­
gani sational measures to address 
poten tial risks by inhalation, dermal 
contact or eye contact. Where those 
risks cannot be eliminate d or reduced 
to a minimum by such procedures and 
measures, the additive and premixtures 
shall be used with personal prot ective 
equipment, including breathing pro­
tectio n, safety glasses and gloves.
2b163- -
or- Turmer ic 
oleoresinAdditive composition
Oleoresin obtained by solvent extraction of 
dried rhizomes of Curcuma long a L.
Char acter isation of the activ e substance
Oleoresin obtained by solvent extraction of 
dried rhizomes of Curcuma long a L. as defined 
by the Council of Europe (4).
Essential oil: 30-33 % (w/w)
Total curcuminoids: 20-35 % (w/w)
— Curcumin (I): 16-21 % (w/w)
— Desmetho xycurcumin (II): 4-6 % (w/w)
— Bis-desmetho xycurcumin (III): 3-5 % 
(w/w).
Humidity: 12-30 % (w/w)All animal 
species- - - 1.The additive shall be incor porat ed into 
the feed in the form of a premixture.
2.In the directions for use of the additive 
and premixtures, the stora ge condi ­
tions and stability to heat treatment 
shall be indicate d.
3.On the label of the additive the follo w­
ing shall be indicate d:
‘Recommended maximum cont ent of 
the active substance per kg compl ete 
feedingstuff with a moisture conte nt 
of 12 %:
— Chick en and laying hens: 30 mg
— Other animal species: 5 mg’.20.4.2031EN Offi cial Jour nal of the European Union 8.3.2022 L 78/31  Analytical method (5)
For the quantifi cation of the phytochemical 
mark er (total curcuminoids) in the feed 
additive (turmer ic oleoresin):
—Spectrophot ometr y – FAO JECF A Com ­
bined Com pendium of Food Additive 
Specifications, ‘Turmer ic Oleoresin’, 
monograph No 1 (2006)4.The functional group, the identif ica­
tion number , the name and the added 
amount of the active substance shall be 
indicated on the label of the premix ­
ture, if the conte nt of the active sub­
stance in complet e feedingstuff speci ­
fied in point 3 is exceeded.
5.The mixture of Turmer ic oleoresin 
with other author ised additives ob­
tained from Curcuma long a L. shall be 
not be allowed in feedingstuffs.
6.For users of the additive and premix ­
tures, feed business operat ors shall es­
tablish operational procedures and or­
gani sational measures to address 
poten tial risks by inhalation, dermal 
contact or eye contact. Where those 
risks cannot be eliminate d or reduced 
to a minimum by such procedures and 
measures, the additive and premixtures 
shall be used with personal prot ective 
equipment, including breathing pro­
tectio n, safety glasses and gloves.
2b163- -
ex- Turmer ic 
extractAdditive composition
Extract of dried rhizomes Curcu ma long a L. 
using organic solvents.
Char acter isation of the activ e substance
Extract of dried rhizomes Curcu ma long a L. 
using organic solvents as defined by the 
Council of Europe (6).
Total curcuminoids: ≥ 90 % (w/w)
— Curcumin (I): 74-79 % (w/w)
— Desmetho xycurcumin (II): 15-19 % (w/w)All animal 
species- - - 1.The additive shall be incor porat ed into 
the feed in the form of a premixture.
2.In the directions for use of the additive 
and premixtures, the stora ge condi ­
tions and stability to heat treatment 
shall be indicate d.
3.On the label of the additive the follo w­
ing shall be indicate d:
‘Recommended maximum cont ent of 
the active substance per kg of compl ete 
feedingstuff with a moisture conte nt of 20.4.2031EN Offi cial Jour nal of the European Union L 78/32 8.3.2022  — Bis-desmetho xycurcumin (III): 2-5 % 
(w/w)
Water: 0,30-1,7 % (w/w)
Einecs number: 283-882-14
FEMA number : 30864
CAS number : 8024-37-14
CoE number: 163
Solid form (powder)
Analytical method (7)
For the quantifi cation of the phytochemical 
mark er (total curcuminoids) in the feed 
additive (turmer ic extract):
—Spectrophot ometr y – FAO JECF A Com ­
bined Com pendium of Food Additive 
Specifications, ‘Curcumin’ , monograph 
No 1 (2006)12 % and milk replacers with a moist ­
ure conte nt of 5,5 %: all species and 
veal calves (milk replacers): 15 mg’.
4.The functional group, the identif ica­
tion number , the name and the added 
amount of the active substance shall be 
indicated on the label of the premix ­
ture, if the conte nt of the active sub­
stance in complet e feedingstuff speci ­
fied in point 3 is exceeded.
5.The mixture of Turmer ic extract with 
other author ised additives obtained 
from Curcuma long a L. shall be not be 
allowe d in feedingstuffs.
6.For users of the additive and premix ­
tures, feed business operat ors shall es­
tablish operational procedures and or­
gani sational measures to address 
poten tial risks by inhalation, dermal 
contact or eye contact. Where those 
risks cannot be eliminate d or reduced 
to a minimum by such procedures and 
measures, the additive and premixtures 
shall be used with personal prot ective 
equipment, including breathing pro­
tectio n, safety glasses and gloves.
(1)Natural sources of flavourings – Repor t No 2 (2007).
(2)The same identif ier applies indiscr iminately to different kinds of extracts and derivatives from Curcuma longa such as turmer ic essential oil, turmer ic extract and turmer ic tincture.
(3)Details of the analytical methods are avail able at the follo wing address of the Reference Laboratory : https://ec.europa.eu/jrc/en/eurl/feed-additives/evaluation-repor ts
(4)Natural sources of flavourings – Repor t No 2 (2007).
(5)Details of the analytical methods are avail able at the follo wing address of the Reference Laboratory : https://ec.europa.eu/jrc/en/eurl/feed-additives/evaluation-repor ts
(6)Natural sources of flavourings – Repor t No 2 (2007).
(7)Details of the analytical methods are avail able at the follo wing address of the Reference Laboratory : https://ec.europa.eu/jrc/en/eurl/feed-additives/evaluation-repor tsEN Offi cial Jour nal of the European Union 8.3.2022 L 78/33  Identifi -
cation 
number 
of the 
additiveName of 
the holder 
of 
author isa-
tionAdditiveComposition, chemical formula, descr iption, 
analytical method.Species or 
category of 
animalMaximum 
ageMinimum 
contentMaximum 
conten t
Other provisionsEnd of period 
of 
author isationmL active substance/kg 
of complet e feed with a 
moisture conten t of 
12 %
Categor y: Sensor y additiv es
Functional group: Flavour ing compounds
2b163-t - Turmer ic 
tinctureAdditive composition
Tincture produced by extraction of ground- 
dried rhizomes of Curcuma long a L. using a  
water/ethanol mixture (55/45 % v/v).
Char acter isation of the activ e substance
Tincture produced by extraction of ground- 
dried rhizomes of Curcuma long a L. using a  
water/ethanol mixture (55/45 % v/v), as 
defined by the Council of Europe (1).
Phenols (as gallic acid equivalent):
1 000 -1 500 μg/mL
Total curcuminoids (2)(as curcumin): 0,04 to 
0,09 % (w/v)
Curcumin (I): 83-182 μg/mL 
Desmetho xycurcumin (II):
80-175 μg/mL
Bis-desmetho xycurcumin (III): 139-224  
μg/mL
Essential oil: 1 176 -1 537 μg/mL
Dry matte r: 2,62-3,18 % (w/w)
Solvent (wate r/ethanol, 55/45): 96-97,5 %  
(w/w)
CoE number:163
Liquid formHorses
Dogs- - - 1.The additive shall be incor porat ed into 
the feed in the form of a premixture.
2.In the directions for use of the additive 
and premixtures, the stora ge condi ­
tions and stability to heat treatment 
shall be indicate d.
3.On the label of the additive the follo w­
ing shall be indicate d:
‘Recommended maximum cont ent of 
the active substance per kg of compl ete 
feedingstuff with a moisture conte nt of 
12 %:
— horses: 0,75 mL;
— dogs: 0,05 mL’.
4.The functional group, the identif ica­
tion number , the name and the added 
amount of the active substance shall be 
indicated on the label of the premix ­
ture, if the conte nt of the active sub­
stance in complet e feedingstuff speci ­
fied in point 3 is exceeded.
5.The mixture of Turmer ic tincture with 
other author ised additives obtained 
from Curcuma long a L. shall be not be 
allowe d in feedingstuffs.20.4.2031EN Offi cial Jour nal of the European Union L 78/34 8.3.2022  Analytical method (3)
For the quantifi cation of the phytochemical 
mark er (total curcuminoids) in the feed 
additive
(turmer ic tincture):
— Spectrophot ometr y (based on European 
Phar macopeia monograph ‘Turmer ic 
Javanese’ (01/2008:1441))6.For users of the additive and premix ­
tures, feed business operat ors shall es­
tablish operational procedures and or­
gani sational measures to address 
poten tial risks by inhalation, dermal 
contact or eye contact. Where those 
risks cannot be eliminate d or reduced 
to a minimum by such procedures and 
measures, the additive and premixtures 
shall be used with personal prot ective 
equipment, including breathing pro­
tectio n, safety glasses and gloves.
(1)Natural sources of flavourings – Repor t No 2 (2007).
(2)Determ ined by spectrophotometry as dicinnamo yl methane derivatives.
(3)Details of the analytical methods are avail able at the follo wing address of the Reference Laboratory : https://ec.europa.eu/jrc/en/eurl/feed-additives/evaluation-repor tsEN Offi cial Jour nal of the European Union 8.3.2022 L 78/35