Reference number  
DRS 414:2019  
© RSB 2019  
 
 
 RWANDA 
STANDARD  DRS  
414 
 
First edition  
2019 -mm-dd 
 
Sugarcane juice — Specification  
 
  DRS 414: 2019  
©RSB 2019  - All rights reserved                  ii 
 In order to match with technological development and to keep continuous progress in industries, 
standards are subject to periodic review. Users shall ascertain that they are in possession of the latest 
edition  
 
 
 
 
 
 
 
 
© RSB 2019  
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized 
in any form or by any means, electronic or mechanical, including photocopying and microfilm, without 
prior written permission from R SB. 
Requests for permission to reprodu ce this document should be addressed to : 
Rwanda Standards  Board  
P.O Box 7099 Kigali -Rwanda  
KK 15 Rd, 49  
Tel. +250 788303492  
Toll Free: 3250  
E-mail: info@rsb.gov.rw  
Website: www.rsb.gov.rw   
ePortal: www.portal.rsb.gov.rw   DRS  414: 2019  
iii                ©RSB 2019  - All rights reserved  
 Contents  Page 
Foreword  ................................ ................................ ................................ ................................ ...................  iv 
1 Scope  ................................ ................................ ................................ ................................ .............  1 
2 Normative references  ................................ ................................ ................................ ...................  1 
3 Terms and definitions  ................................ ................................ ................................ ..................  2 
4 Requirements  ................................ ................................ ................................ ................................  2 
4.1 Raw materials  ................................ ................................ ................................ ...............................  2 
4.2 Optional ingredients  ................................ ................................ ................................ .....................  2 
4.3 General requirements  ................................ ................................ ................................ ..................  3 
4.4 Specific requirements  ................................ ................................ ................................ ..................  3 
5 Food additives  ................................ ................................ ................................ ..............................  3 
6 Hygiene  ................................ ................................ ................................ ................................ ..........  3 
7 Contaminants  ................................ ................................ ................................ ................................  4 
7.1 Pesticide residues  ................................ ................................ ................................ ........................  4 
7.2 Heavy metal contaminants  ................................ ................................ ................................ ..........  4 
8 Packaging  ................................ ................................ ................................ ................................ ...... 4 
9 Labelling  ................................ ................................ ................................ ................................ ........  4 
10 Sampling  ................................ ................................ ................................ ................................ ....... 5 
Annex  A (normative)  Method of sampling for Sugarcane juice ................................ ...........................  6 
A.1 Definitions  ................................ ................................ ................................ ................................ ..... 6 
A.2 Sampling plans  ................................ ................................ ................................ .............................  6 
A.3 Scale of sam pling  ................................ ................................ ................................ .........................  8 
A.4 Test samples and reference samples  ................................ ................................ .........................  8 
A.4.1  Samples for microbiological tests  ................................ ................................ ..............................  8 
A.4.2  Samples for other tests ................................ ................................ ................................ ................  8 
 DRS 414: 2019  
©RSB 2019  - All rights reserved                  iv 
 Foreword   
Rwanda Standard s are prepared  by Technical Committees and approved by R wanda Standards Board (RSB) 
Board  of Directors in  accordance with the procedures of RSB, in compliance with Annex 3 of the WTO/TBT 
agreement on the preparation, adoption and application of standards.  
The main task of technical committees is to prepare national standards.  Final Draft Rwanda S tandards adopted 
by Technical committ ees are ratified  by me mbers of RSB Board of Director s for publication and gazettment as 
Rwanda Standards.  
DRS 414 was prepared by Technical Committee RSB/TC 001, Non-alcoholic beverages . 
In the preparation of this standard, reference was made to the following standard:  
1) IS 12923 : Cane gur (jaggery) —Specification  
The assistance de rived from the above source is hereby acknowledged with thanks.  
Committee membership  
The following organizations were represented on the Technical Committee on Non-alcoholic beverages 
(RSB/TC 001) in the preparation of this standard.  
African Society of Commerce and Management Ltd (ASCOM)  
Amazon Nutrition Cabinet Ltd  
Bona Natural Fruits Tr ansformation Company Ltd (B.N.F.T.C)  
CFRDTA Ltd  
College of Agriculture, Animal Science and Veterinary Medicine (UR -CAVM)  
International Institute of Tropical Agriculture (IITA)  
KAMAIMU Ltd  
Nyarutarama Business Incubation Cente r (NBIC)  
Rwanda  Standards Boar d (RSB) – Secretariat  
 DRS  414: 2019  
1                ©RSB 2019  - All rights reserved  
 Sugarcane juice  — Specification  
1 Scope   
This Draft Rwanda St andard  specifies the requirements, sampling and test methods for sugarcane juice 
intended for human consumption . 
2 Normative references   
The following documents are referred to in the text in such a way that some or all of  their content constitutes 
requirements of  this document.  For dated references, only the edition  cited applies. For undated references, the 
latest edition of the referenced document (including any amendments) applies.  
AOAC 950.17, Official Method Citric Acid in Non -alcoholic Beverages  
AOAC 950.21,  Official Method Acidity (Volatile) in Non -alcoholic Beverages  
AOAC 999.10,  Official Method Lead, Cadmium, Zinc, Copper, and Iron  in Foods —Atomic Absorption 
Spectrophotometry  
RS CAC/RCP 1, General principles of food hygiene —Code of practice  
RS CODEX STAN 192, General standard for food additives  
RS EAS 12, Potable water — Specification  
RS EAS 38, Labelling of pre -packaged foods —General requirements  
RS ISO 16649 -2, Microbiology of food and animal feeding stuffs — Horizontal method for the enumeration of 
beta-glucuronidase -positive Escherichia coli — Part 2: Colony -count technique at 44 degrees C using 5 -bromo -
4-chloro -3-indolyl beta -D-glucuronide  
RS ISO 1842,  Fruit and vegetabl e products — Determination of pH  
RS ISO 21527 -1, Microbiology of food and animal f eeding stuffs — Horizontal method for the enumeration of 
yeasts and moulds — Part 1: Colony count technique in products with water activity greater than 0,95  
RS ISO 2173, Fruit and vegetable products — Determination of soluble solids — Refractometric metho d 
RS ISO 2448 , Fruit and vegetable products -- Determination of ethanol content  
RS ISO 4833 -1, Microbiology of food and animal feeding stuffs — Horizontal method for the enumeration of 
microorganisms — Part 1: Colony count at 30 degrees C by the pour plate technique   
©RSB 2019  - All rights reserved                  2 
 RS ISO 6579 -1, Microbiology of the food chain — Horizontal method for the detection , enumeration and 
serotyping of Salmonella — Part 1: Detection of Salmonella spp.  
RS ISO 6634 , Fruits, vegetables and derived products -- Determination of arsenic content -- Silver 
diethyldithiocarbamate spectrophotometric method  
RS ISO 6888 -1, Microbiolog y of food and animal feeding stuffs — Horizontal method for the enumeration of 
coagulase -positive staphylococci (Staphylococcus aureus and other species) — Part 1: Technique using Baird -
Parker agar medium  
3 Terms and definitions   
For the purposes of this standard, the follow ing terms and definitions apply .  
3.1 
sugarcane juice  
non-fermented but ferment able liquid obtained from sound, appropriately mature and sugarcane  
3.2  
extraneous matter  
inorganic matter such as sand, glass, metal, gravel, dirt, pebbles, stones, lumps of earth, clay and mud and 
organic matter such as chaff, straw, weed seeds and grains of crops, insects or insects’ fragments, rodent hairs 
or any other foreign matter  
3.3  
food grade packaging material  
packaging material made of su bstances which are suitable for their intended use and which will not alter the 
quality, safety and organoleptic  properties  of the product  
4 Requirements  
4.1 Raw materials  
4.1.1  Sugarcane juice shall be manufactured from sound, appropri ately mature sugarcane . 
4.1.2 Sugarcane juice content shall  at least be 60% of the whole mixture.   
4.2 Optional ingredients  
The following ingredients may be used in the manufacture of sugarcane juice and shall comply with relevant 
standards:  DRS  414: 2019  
3                ©RSB 2019  - All rights reserved  
 a) potable water complying with RS EAS 12;  
b) lemo n juice  complying with RS  202;  
c) ginger  complying with  RS 303 -1 or RS 303 -2; and 
d) garlic complying with RS 371 or RS 372 . 
4.3 General requirements   
Sugarcane juice shall:  
a) be free from any substances injurious to health;  
b) be free from any extraneous matter;  
c) be free from foreign material such as grit and dirt;  
d) have the characteristic appearance, taste and aroma of sugarcane juice;  and 
e) be free from signs of fermentation.  
4.4 Specific requirements  
Sugarcane juice shall comply with the specific requirements in Table 1 when tested in accordance with the 
methods specified therein.  
Table 1— Specific requirements for Sugarcane juice  
S/N Characteristic  Requirement  Test method  
i.  Total Soluble So lids, (Brix), min.  18 RS ISO 2173  
ii.  pH 4.0 - 5.0 RS ISO 1842  
iii.  Total Acidity, g/l max.  0.7 AOAC 950.17  
iv.  Ethanol content, % v/v , max  0.3 RS ISO 2448  
v.  Volatile acidity as acetic acid, g/l, max  Absent  AOAC 950.21  
5 Food additives  
Sugarcane juice may contain o nly permitted food additives in accordance with RS CODEX STAN 192.  
6 Hygiene  
6.1 Sugarcane juice  shall be produced and handled under hygienic conditions in accordance with RS 
CAC/RCP 1.   
©RSB 2019  - All rights reserved                  4 
 6.2 Sugarcane juice shall comply with microbiological limits given in Table 2 when tested in accordance with 
the methods specified therein . 
Table 2 — Microbiological limits in Sugarcane juice  
S/N Microorganism  Limit  Test method  
i.  Total Viable Count, cfu/ml  103 RS ISO 4833 -1 
ii.  Escheric hia coli , cfu/ml  Absent  RS ISO 16649 -2 
iii.  Salmonella spp in 25 ml  Absent  RS ISO 6579 -1 
iv.  Staphylococcus aureus,  cfu/ml  Absent  RS ISO 6888 -1 
v.  Yeasts and moulds , cfu/ml  102 RS ISO 21527 -1 
7 Contaminants  
7.1 Pesticide residues  
Sugarcane juice shall comply with the pesticide residue limits prescribed by the Codex Alimentarius Commission 
of the respective commodity.  
7.2 Heavy metal contaminants  
Sugarcane juice shall not contain heavy metal contaminants in excess of the limits in Table 3 when tested in 
accordance with the methods specified therein.  
Table 3 —Heavy metal limits in Sugarcane juice  
S/N Heavy metal  Maximum limit (mg/kg)  Test method  
i.  Arsenic (As)  0.2 RS ISO 6634  
ii.  Lead (Pb)   0.05  
AOAC 999.10  iii.  Cadmium (Cd)   0.05  
8 Packaging  
Suga rcane juice shall be packaged in  food grade packaging material that ensures the integrity and safety of the 
product.  
9 Labelling  
In addition to the requirements of RS EAS 38, the following specific labelling requirements shall apply and shall 
be legibly and indelibly marked:  
a) name of the product as “Sugarcane juice” ; 
b) name and physical address of the manufacturer / packer;  DRS  414: 2019  
5                ©RSB 2019  - All rights reserved  
 c) list of ingredients  in desc ending order;  
d) date of manufacture;  
e) expiry date;  
f) batch /lot number;  
g) brand /trade name; if any;  
h) net content;  
i) instructions for use;  
j) storage conditions; and  
k) country of origin.  
10 Sampling  
Sampling for sugarcane juice shall be done in accorda nce with Annex A.   
©RSB 2019  - All rights reserved                  6 
 Annex  A 
(normative)  
 
Method of sampling for Sugarcane juice  
A.1 Definitions  
A.1.1  
lot  
collection of primary containers or units of the same size, type, and style manufactured or packed under similar 
conditions and handled as a single unit of trade  
A.1.2   
lot size  
number of primary containers or units in the lot  
A.1.3  
sample size  
total number of sample units drawn for examination from a lot  
A.1.4  
sample unit  
container, a portion of the contents of a container, or a composite mixture of product from small containers that 
is sufficient for the examination or testing as a single unit. For fill of container, the sample unit shall be the entire 
contents of the container  
A.2 Sampling plans  
Sampling shall be done in accordance with the plan specified in Table A.1. 
Table A.1 — Sampling plan  
Lot size (primary containers)  Size of container,  
n a 
Net weight equal to or less than 1 kg (2.2 lb)  
4 800 or less  13 DRS  414: 2019  
7                ©RSB 2019  - All rights reserved  
 Lot size (primary containers)  Size of container,  
n a 
4 801 to 24 000  21 
24 001 to 48 000  29 
48 001 to 84 000  48 
84 001 to 144 000  84 
144 001 to 240 000  126 
Over 240 000  200 
Net weight greater than 1 kg (2.2 lb) but not more than 4.5 kg (10 lb)  
2 400 or less  13 
2 401 to 15 000  21 
15 001 to 24 000  29 
24 001 to 42 000  48 
42 001 to 72, 000  84 
72 001 to 120 000  126 
Over 120 000  200 
Net weight greater than 4.5 kg (10 lb) 
600 or less  13 
601 to 2 000  21 
2 001 to 7 200  29 
7 201 to 15 000  48 
15 001 to 24 000  84 
24 001 to 42 000  126 
Over 42 000  200 
a n = number of primary containers in sample.  
  
©RSB 2019  - All rights reserved                  8 
 A.3 Scale of sampling  
A.3.1  All bottles in a consignment belonging to the same batch of manufacture shall constitute a lot. If the 
consignment is declared to consist of different batches of manufacture, bottles of the same batch shall be 
grouped together and each group so formed shal l constitute a separate lot and each lot shall be sampled.  
A.3.2  The number of bottles to be selected from a lot for testing for microbiological and other requirements 
shall depend on the size of the lot and shall be in accordance with Table A. 2. 
Table A. 2 — Number of bottles to be selected for sampling  
No. of bottles in the lot (L)  No. of bottles to be selected  
Microbiological testing  Other tests  
L ≤ 1 300  12 18 
1 300 < L ≤ 3 200  18 24 
L > 3 200  24 30 
A.3.3  The bottle to be selected for testing shall be chosen at random from the lot by the following procedure. 
Starting from any bottle, count them as 1, 2, 3... up to r. Every rth bottle thus counted shall be withdrawn r being 
the integral part of N/n, where N is the total number of bottles in the lot and n is the total number of bottle to be 
chosen.  
A.4 Test samples and reference samples  
A.4.1  Samples for microbiological tests  
The sample bottle selected for microbiological tests (see Table A. 2) shall be divided at random into three equal 
sets and labelled with all particulars of sampling. One of these sets of sample bottles shall be for the purchaser; 
another for the vendor and the third set is the reference.  
A.4.2  Samples for other tests  
The sample bottles selected for other tests (see Table A.2) shall be divide d at random into three equal sets and 
labelled with all the particulars of sampling. One of these sets of sample bottles shall be for the purchaser, 
another for the vendor and third is the reference.  
 
  DRS 414 : 2019  
ICS 67.160.20  
 
 
©RSB 2019  - All right s reserved