ICS 71.100.35  
 
Reference number  
DRS 456 -3: 2021  
© RSB 2021  
  
 RWANDA 
STANDARD  DRS  
456-3 
 
First edition  
Approved by RSB  2021 -mm-dd 
 
Surface d isinfectants  — Specification  
Part 3: 
Disinfectants based on glutaraldehyde  
   
DRS 456 -3: 202 1 
i                ©RSB 2021  - All rights reserved  
 In order to match with technological development and to keep continuous progress in industries, 
standards are subject to periodic review. Users shall ascertain that they are in possession of the latest 
edition 
 
 
 
 
 
 
 
 
© RSB 2021  
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized 
in any form or by any means, electronic or mechanical, including photocopying and microfilm, without 
prior written permission  from R SB. 
Requests for permission to reproduce this document should be addressed to : 
Rwanda Standards  Board  
P.O Box 7099 Kigali -Rwanda  
KK 15 Rd, 49  
Tel. +250 788303492  
Toll Free: 3250  
E-mail: info@rsb.gov.rw  
Website:  www.rsb.gov.rw  
ePortal: www.portal.rsb.gov.rw   DRS 456-3: 2021  
©RSB 2021  - All rights reserved                  ii 
 Contents  Page 
1 Scope  ................................ ................................ ................................ ................................ ............  1 
2 Normative references  ................................ ................................ ................................ ..................  1 
3 Terms and definitions  ................................ ................................ ................................ .................  1 
4 Requirements  ................................ ................................ ................................ ...............................  2 
4.1 Types  ................................ ................................ ................................ ................................ .............  2 
4.2 Raw materials  ................................ ................................ ................................ ...............................  2 
4.3 General requirements  ................................ ................................ ................................ ..................  2 
4.4 Specif ic requirements  ................................ ................................ ................................ .................  2 
5 Packaging and Labelling  ................................ ................................ ................................ .............  3 
5.1 Packaging  ................................ ................................ ................................ ................................ ..... 3 
5.2 Labelling  ................................ ................................ ................................ ................................ ....... 3 
6 Sampling  ................................ ................................ ................................ ................................ ....... 4 
6.1 General  ................................ ................................ ................................ ................................ ..........  4 
6.2 Sample for inspection  ................................ ................................ ................................ .................  4 
6.3 Sample for testing ................................ ................................ ................................ ........................  4 
Annex  A (normative)  Determination of corrosiveness  ................................ ................................ ........  6 
A.1 Test strips  ................................ ................................ ................................ ................................ ..... 6 
A.2 Procedure  ................................ ................................ ................................ ................................ ..... 6 
Annex  B (normative)  Determination of water -insoluble matter  ................................ ..........................  7 
B.1 Procedure  ................................ ................................ ................................ ................................ ..... 7 
B.2 Calculation  ................................ ................................ ................................ ................................ .... 7 
Annex  C (normative)  Determination of storage stability  ................................ ................................ ..... 8 
 
 DRS 456 -3: 202 1 
iii                ©RSB 2021  - All rights reserved  
 Foreword  
Rwanda Standard s are prepared  by Technical Committees and approved by R wanda Standards Board (RSB) 
Board  of Directors in  accordance with the procedures of RSB, in compliance with Annex 3 of the World Trade 
Organisation/Technical Barrier to Trade ( WTO/TBT ) agreement on the preparation, adoption and application of 
standards.  
The main task of technical committees is to prepare national standards.  Final Draft Rwanda  Standards adopted 
by Technical committ ees are ratified  by me mbers of RSB Board of Director s for publication and gazettment as 
Rwanda Standards.  
DRS 456 -3 was prepared by Technical Committee RSB/TC 024, Organic and Inorganic Chemicals.  
In the preparation o f this standard, reference was made to the following standard:  
US 1710 : Disinfectants/sanitizers based on glutaraldehyde for general use — Specification  
The assistance de rived from the above source is hereby acknowledged with thanks.  
DRS  456 consists of the following parts, under the general title Surface disinfectants — Specification : 
 Part 1: Disinfectants for general use  
 Part 2: Disinfectants based of iodophors  
Part 3: Disinfectants based on glutaraldehyde  
Committee membership  
The follow ing organizations were represented on the Technical Committee on Organic and Inorganic Chemicals 
(RSB/TC 024) in the preparation of this standard.  
Star Construction and Consultancy Ltd  
Rwanda Inspectorate, Competition and Consumer Protection Authority  
Rwan da Food and Drugs Authority  
Rwanda Investigation Bureau  
Rwanda Forensic Laboratory  
Rwanda Social Security Board  
Rwanda Environment Management Authority  DRS 456-3: 2021  
©RSB 2021  - All rights reserved                  iv 
 BARANYUZWE Cosmetics Ltd  
SULFO Rwanda Industries Ltd  
UBURANGA Natural Products  
Divine Hope Company Ltd  
University of Rwanda /College of Medicine and Health Sciences  
University of Rwanda /College of Sciences and Technology  
University of Rwanda /College of Education  
Rwanda Polytechnic – IPRC Kigali  
Standards for Sustainability (SfS)  
Rwanda  Standards Board  (RSB) – Secretariat  
  DRS 456 -3: 202 1 
v                ©RSB 2021  - All rights reserved  
  DRS 456-3: 2021  
1                ©RSB 2020  - All rights reserved  
 
 Surface disinfectants — Specification — Part 3: Disinfectants based on 
glutaraldehyde  
1 Scope  
This Draft  Rwanda Standard  specifies the requirements, sampling and test methods for two types of 
disinfectants/sanitizers based on glutaraldehyde and intended for general use on inanimate surfaces.  
2 Normative references  
The following documents are referred to in the text in such a way that some or all of  their content constitutes 
requirements of this document.  For dated references, only the edition  cited applies. For undated references, the 
latest edition of the reference d document (including any amendments) applies.  
EAS 384 , Disinfectants  — Glossary of terms  
DRS  457, Determination of bactericidal efficacy of disinfectants/sanitizers  
3 Terms and definitions  
For the purposes of this standard, the terms and definitions given in EAS 384 and the following apply.  
3.1 
batch  
collection of packages containing a disinfectant of a single type and composition and of a single manufactured 
blend, or of a single delivery.  
3.2 
inanimate surface  
any surface other than live human or live ani mal tissue (for example, skin)  
3.3 
lot 
quantity of disinfectant in sealed containers of the same size and bearing the same batch identification, from 
one manufacturer, submitted at any time for inspection and testing  
3.4  
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 glutaraldehyde  
chemical that is used as  a cold sterilant to disinfect and clean heat -sensitive medical, surgical and dental 
equipment.  
4 Requirements  
4.1 Types  
The product shall be of one of the following types, as required:  
a) Type 1 : a homogeneous liquid; or  
b) Type 2 : a homogeneous liquid that has to be mixed with either a solid or a liquid activator b efore use as a 
disinfectant . 
4.2 Raw materials  
The product shall not contain ingredients that are recognized as being potentially hazardous or toxic when the 
products are used in accordance with the ma nufacturer’s recommendations, nor shall form toxic of potentially 
toxic reaction products.  
4.3 General requirements  
4.3.1  The product shall not:  
a) leave an objectionable odour on surfaces; and  
b) impart any colour, taste, odour or flavour to f ood products of drinki ng water, when used in accordance with 
the manufacturer’s recommendations.  
4.3.2  The product intended for use on food contact surfaces shall not contain perfumes.  
4.3.3  Stability after dilution of type 1 product and after activation of type 2 product  
After  a type 1 product has been diluted to the prescribed concentration or after the two components of a type 2 
product have been mixed in accordance with the manufacturer’s instructions and the product has been stored 
in closed dark containers at 25 °C for the  effective life as stated on the label, the resulting dilution shall still 
comply with the requirements.  
4.4 Specific requirements  
The product shall also comply with the specific requirements given in the table 1 when tested in accordance 
with the correspondin g test method.  
Table 1 – Specific requirements for surface disinfectants based on glutaraldehyde  
S/N Characteristic Requirement Test method  
3                ©RSB 2021  - All rights reserved  
 Type 1  Type 2  
i) 
Corrosiveness  No evidence of pitting, etching or 
discolouration  Annex A  
ii) Water insoluble matter content, g/L, max.  1 Annex B  
iii) Storage stability  Homogeneous 
and free -flowing  Free flowing  Annex C 
iv) Bactericidal efficacy a To pass the test  DRS 457  
a It is recommended that the user assesses the efficacy of the disinfectant and suitability  for purpose for the specific target surfaces under 
local conditions.   
5 Packaging and Labelling  
5.1 Packaging  
5.1.1  The container, including the closure, in which the product is packaged shall not interact chemically or 
physically with the product and shall be strong enough to protect the product adequately during normal handling, 
transportation and storage.  
5.1.2  The closure shall not be made of cork or of any material that contains cork.  
5.1.3  Only packs of the same size and bearing the same batch identific ation shall be packaged together in 
a bulk pack . 
5.2 Labelling  
The following information shall appear prominently, legibly and indelibly on each product container or on a label 
securely attached to each container:  
a) Name and full address of the manufacturer, and  trademark if any;  
b)  Name of the product as “disinfectant/sanitizer based on glutaraldehyde”;  
NOTE  The product name should not be misleading to the consumer.  
c) A statement of the nominal volume or mass of the contents in metric units;  
d) A description of how the  components of a type 2 disinfectant/sanitizer are to be mixed;  
e) general instructions for use of the product. The instructions shall include the recommended concentration, 
dilution level and the minimum exposure period for each purpose;  
f) hazard and toxicity warnings, where relevant;  
g) hazard and toxicity warnings, where relevant;  
h) statement about the safety precautions to be taken when using the product and the first aid steps to be 
taken in case of direct ingestion or skin contact;   
©RSB 2021  - All rights reserved                  4 
 
 i) the batch identification numb er; 
j) manufacture and expiry dates;  
k) adequate draining, rinsing and/or drying requirements from surfaces after use;  
l) appropriate instructions for the storage of the product, including a warning to store away from children;  
m) when the prescribed end -use concentration of the product is above 0.2% glutaraldehyde, warnings that:  
1) the product can be detrimental to the skin, and that gloves should be used; and  
2) the product should not be used on surfaces that will come into contact with food.  
NOTE  The manufacture r should substantiate any virucidal claim made about the product.  
6 Sampling  
6.1 General  
The following sampling procedure shall be applied in determining whether a lot submitted for  inspection and 
testing complies with the relevant requirements of this standard.  The sample so  drawn shall be deemed to 
represent the lot.  
6.2 Sample for inspection  
After inspecting the lot for compliance with  Clause 4,  take, at random, the number of containers, as  relevant, 
shown in column 2 of Table 2, relative to the appropriate lot size shown in column 1.  
Table 2 — Samples for inspection and testing  
Lot size 
(number of containers) Sample size for ph ysical 
examination 
(number of containers) Sample size for micro biological 
examin ation 
(number of containers) 
0 to 5 000  3 3 
5 001  to 12  500 6 3 
12 501 to 25 0 00 9 3 
25 001 to 50 0 00 16 3 
50 001 upwards 30 3 
6.3 Sample for testing  
After inspection of the containers taken in accordance with 6.2, 
a) take, at random, half the number of containers and use them for the storage stability test; and   
5                ©RSB 2021  - All rights reserved  
 b) thoroughly mix the contents of each of the remaining containers and, take from each container the lesser 
of the total volume and 250 mL, and obtain a composite test sample by combining and thoroughly mixing 
these quantities. Use these samples for testing f or compliance with the requirements of Clause 4.  
 
  
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 Annex  A 
(normative)  
 
Determination of corrosiveness  
A.1 Test strips  
Use two strip, each of size approximately 75 mm x 19 mm x 1.5 mm, of bright -finished uncoated aluminium 
Type EN AW 1050A  
A.2 Procedure  
A.2.1  Degrease the aluminium test strips by washing in a mixture of equal volumes of ethanol and acetone. 
Allow the strips to air -dry, then heat them for 15 min in an oven at 105 °C ± 5 °C and allow them to cool in a 
desiccator.  
A.2.2  Prepare the test solution o f the product under test, at the highest concentration recommended by the 
manufacturer, and transfer 250 ml f the freshly prepared solution to a suitably stoppered glass bottle.  
A.2.3  Completely immerse the test strips  in the solution in the glass bottle. Stopper the bottle and maintain 
the bottle at 25 °C ± 2 °C for 16h.  
A.2.4  Remove the test strips from the test solution, rinse them thoroughly with water and then acetone and 
allow them to air -dry. Then heat the test strips for 30 min in an oven at 105 °C ± 5 °C and cool them again in a 
desiccator.  
A.2.5  Remove the test strips from the desiccator and examine them for compliance with 4.4.1.  
 
   
7                ©RSB 2021  - All rights reserved  
 Annex  B 
(normative)  
 
Determination of water -insoluble matter  
B.1 Procedure  
Pipette 5.0 ml of the undiluted product into a beake r and add 250 ml of standard hard water.  
Heat the solution in a steam bath with frequent stirring until the sample is completely dispersed.  
Filter the solution immediately, under suction, through a tarred 1.6 -µm glass fibre filter and ensure that the 
insoluble matter is quantitatively transferred to the filter.  
Wash the beaker and the residue five times with 20 -ml volumes of hot standard hard water. Wash the filter with 
distilled water (to remove salts from the hard water).  
Allow the solution to drain compl etely and dry residue at 105 °C ± 2 °C until constant mass is attained. Cool in 
a desiccator and weigh.  
B.2 Calculation  
The water -insoluble matter content in the test solution, expressed in grams per litre (g/l), shall be calculated 
using the following formula:  
  Water -insoluble matter content = 𝑚
𝑉 
Where,  
 m is the mass, in grams, of the residue after it has been dried; and  
 V is the volume, in litres, of the test solution.   
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 Annex  C 
(normative)  
 
Determination of storage stability  
C.1 Store type a product and the liquid component of type 2 product in their original unopened containers at 
5 °C ± 1 °C. inspect the contents of half the packages in the sample for compliance with the relevant 
requirements of 4.4.3.  
C.2 Store the solid compon ent of type 2 product in its original unopened containers under ambient conditions 
for six months. The liquid component of the product doe s not have to be stored under these conditions. Inspect 
the co ntents of half of the containers in the sample for compl iance with 4.4.3.2 and test the solid component, in 
conjunction with the type 2 product that it activates, for compliance with 4.4.5.  
  
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 Bibliography  
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