COMMISSION IMPLEMENTING REGUL ATION (EU) 2020/1201 
of 14 August 2020 
as regards measures to prev ent the introduction into and the spread within the Union of Xylella 
fastidiosa (Wells et al.) 
THE EUR OPEAN COMMISSION, 
Havi ng regar d to the Treaty on the Functioning of the European Union, 
Havi ng regard to Regulation (EU) 2016/2031 of the European Parliament and of the Council of 26 October 2016 on 
prote ctive measures against pests of plants, amending Regulations (EU) No 228/2013, (EU) No 652/2014 and 
(EU) No 1143/2014 of the European Parliament and of the Council and repealing Council Directives 69/464/EEC, 
74/647/EEC, 93/85/EEC, 98/57/EC, 2000/29/EC, 2006/91/EC and 2007/33/EC (1), and in particular Article 28(1) and (2) 
thereof, 
Whereas: 
(1) Commission Implementing Decision (EU) 2015/789 (2) has been applied since May 2015 with the aim to prevent 
further spread within and introduction into the Union of Xylella fastidiosa (Wells et al.) (‘the specified pest’) . That 
Implementing Decision has been updated οn several occasions to take into account the scientific and technical 
evidence made available at the time. 
(2) On 15 May 2019, the European Food Safet y Author ity (‘the Author ity’) published a new Scientific Opinion (3) on the 
risk to plant health posed by the specified pest in the Union territory, focusing on poten tial establishment, shor t‐ and 
long‐range spread, the length of the asymp tomat ic period, the impact of the specifi ed pest and an update on risk 
reduction options. On 15 May 2019, the Auth ority published a scientific repor t(4) on the effectiveness of in planta 
control measures for the specifi ed pest, confir ming as previously stated that there are currently no control measures 
available to eliminate the specified pest from a diseased plant in open field conditions. On 25 June 2019, the 
Authority published a pest survey card (5) on the specified pest provid ing further elements to ensure more target ed 
survey activities. On 28 Apr il 2020, the Author ity updated the database of plant species found infected by the 
specifi ed pest worldwide (6). Moreo ver, on 8 June 2020, the Auth ority published the Guidelines for statistically 
sound and risk-based surveys of Xylella fastidiosa (7) that will assist Member States to implement the conf idence level, 
the design prevalence proposed and preparation of the survey activities to be carried out within and outside the 
demarcate d areas, in the Union territory . 
(3) The recent scientific developments, the outcome of the audits carried out by the Commission in the Member States 
concer ned and the exper ience gained during the implementation of Implementing Decision (EU) 2015/789 indicate 
the need to update those measures in order to ensure a more targe ted approach for the control of the specified pest 
in the Union territory . 
(1)OJ L 317, 23.11.2016, p. 4. 
(2)Commission Implementing Decision (EU) 2015/789 of 18 May 2015 as regards measures to prevent the introduction into and the 
spread within the Union of Xylella fastidiosa (Wells et al.) (OJ L 125, 21.5.2015, p. 36). 
(3)EFSA Jour nal 2019;17(5):5665, 200 pp. https://doi.org/10.2903/j.efsa.2019.5665 
(4)EFSA Jour nal 2019;17(5):5666, 17 pp. https://doi.org/10.2903/j.efsa.2019.5666 
(5)EFSA Suppor ting publication 2019:EN-1667. 53 pp. https://doi.org/10.2903/sp.efsa.2019.EN-1667 
(6)EFSA Jour nal 2020;18(4):6114. https://doi.org/10.2903/j.efsa.2020.6114 
(7)EFSA Suppor ting publication 2020:EN-1873. 76 pp. https://doi.org/10.2903/sp.efsa.2020.EN-1873 EN Offi cial Jour nal of the European Union L 269/2                                                                                                                                           17.8.2020   (4) For the purpose of clarity, the definit ion of host plants should refer to all the plant species or genera found infected 
by the specifi ed pest worldwide, while the definition of specified plants should only refer to those host plants whic h 
have been found infected by a particular subspecies of the specifi ed pest. In this conte xt, and in order to ensure a 
certain propor tionality , some measures of this Regulation should only apply to host plants and some others only to 
specifi ed plants. 
(5) In order to ensure a bette r overview of the presence of the specifi ed pest, Member State s should intensify the annual 
surveys for that presence in line with the lates t scientific and technical information. 
(6) In order to eradicat e the specifi ed pest and prevent its further spread in the rest of the Union, Member State s should 
establish demarcat ed areas consisting of an infected zone and a buffe r zone, and apply eradication measures. The 
width of the buffe r zones should be propor tionate to the level of risk and the spreading capacity of the vect or. 
(7) However , in cases of isolat ed occur rences of the specified pest, the establishment of a demarcate d area should not be 
required if the specified pest can be eliminated from those plants, recently introduced, where it was found to be 
present. This will be the most propor tionate approach as long as the surveys carried out in the area concer ned lead 
to the conclusion that the specifi ed pest has not been established. 
(8) In certain areas of the Union, it is appropr iate to conclude that the eradication of the specifi ed pest is no longer 
possible. The Member States concer ned should theref ore be allowed to apply measures for the containment of that 
pest within those areas, instead of eradication. Those measures should be less stringent than the measures for 
eradication, but they should ensure a diligent survey approac h and more precautionar y actions in the respective 
buffe r zones and at the outer part of the infect ed zones, in order to prevent the spread of the specifi ed pest to the 
rest of the Union territory . 
(9) In order to ensure the immediate remo val of the infected plants and prevent further spread of the specifi ed pest in 
the rest of the Union territory , the monitori ng of the demarcat ed areas should be carried out annually at the most 
appropr iate time of the year. The monitoring should also focus on vect ors present in the demarcate d area so as to 
determine the risk of further spreading by vectors and to evaluate the effectiveness of the phytosan itary control 
measures applied against the vect or population, in all its stages . 
(10) Exper ience conf irms, in line with the latest scientifi c evidences, that vector control, together with the immediate 
remo val of the bacteri al inoculum, is key to prevent further spread of the specified pest within the rest of the Union 
territory . Theref ore, Member States should ensure proper implementation of the phytosan itary treatments, prior to 
the remo val of the plants, as well as during the year, again st the vect or population, in all its stages. Those treatments 
should include effic ient chemical, biological or mech anical treatments against the vect ors, with preference to non- 
chemical solutions, wherever possible. 
(11) Plant species kno wn to be suscept ible to the subspecies of the specified pest detected in the demarcated area and 
which have been grown for at least part of their life in that demarcated area, or which have been move d through 
such an area, are more likely to have been infected with the specified pest. Theref ore, it is propor tionat e to establish 
special requirements for the movement within the Union only as regards specified plants. It is also propor tionate to 
apply less stringent requirements for the mov ement of those plants within the infected zones, or from the infec ted 
zones to the buffer zones, than the requirements applied for their move ment out of the demarcated areas. 
(12) With the aim of facilitating trade while protecting plant health, and taking into account the nature of the specif ied 
pest, host plants originating in a third countr y where that pest is not present should, when introduced into the 
Union, be accompan ied by a phytosan itary certificate including an additional declaration stating that that countr y is 
free from the specifi ed pest. EN Offi cial Jour nal of the European Union 17.8.2020                                                                                                                                           L 269/3   (13) In order to ensure that host plants introduced into the Union from areas of third countr ies where the specified pest is 
known to be present, are free from the specified pest, the requirements for their introduction into the Union should 
be similar to those set out for mov ement of specif ied plants originating in demarcated areas. 
(14) In light of the lates t scientifi c and technical evidence, survey activities carried out by third countr ies to conf irm the 
absence of the specified pest in areas of their territory or in production sites author ised for expor t to the Union 
should be also intensi fied. 
(15) Rules should be established concer ning official checks on movements of the specifi ed plants out of the demarcate d 
areas to the rest of the Union territory, and the introduction into the Union of host plants from third countr ies. 
Those rules should be propor tionate, effective and take into account the provisions of Regulation (EU) 2017/625 of 
the European Parliament and of the Council (8), whic h became applicable on 14 December 2019. 
(16) Implementing Decision (EU) 2015/789 should theref ore be repealed. 
(17) As rega rds survey activities carried out in areas of the Union territory where the pest is known not to occur or where 
derogat ion for the establishment of the demarcated areas is applied, as well as in areas or production sites of third 
countr ies, it is appropr iate to allow sufficient time to Member States and third countr ies to design those activities so 
that they provide the level of statistical confidence required. The requirements relating to those survey activities 
should theref ore apply from 1 Januar y 2023. 
(18) The measures provid ed for in this Regulation are in accordance with the opinion of the Standing Committ ee on 
Plants, Animals, Food, and Feed, 
HAS ADOPTED THIS REGUL ATION: 
CHA PTER I 
DEFINITIONS 
Article 1 
Def initions 
For the purposes of this Regulation, the followi ng definitions shall apply: 
(a) ‘specif ied pest’ means Xylella fastidiosa (Wells et al.) and any of its subspecies; 
(b) ‘host plants’ means all plants for planting, other than seeds, belonging to the genera or species listed in Annex I; 
(8)Regulation (EU) 2017/625 of the European Parliament and of the Council of 15 March 2017 on official controls and other official 
activities perfor med to ensure the application of food and feed law, rules on animal health and welfare, plant health and plant 
prot ection products, amending Regulations (EC) No 999/2001, (EC) No 396/2005, (EC) No 1069/2009, (EC) No 1107/2009, 
(EU) No 1151/2012, (EU) No 652/2014, (EU) 2016/429 and (EU) 2016/2031 of the European Parliament and of the Council, Council 
Regulations (EC) No 1/2005 and (EC) No 1099/2009 and Council Directives 98/58/EC, 1999/74/EC, 2007/43/EC, 2008/119/EC 
and 2008/120/EC, and repealing Regulations (EC) No 854/2004 and (EC) No 882/2004 of the European Parliament and of the 
Council, Council Directives 89/608/EEC, 89/662/EEC, 90/425/EEC, 91/496/EEC, 96/23/EC, 96/93/EC and 97/78/EC and Council 
Decision 92/438/EEC (Official Controls Regulation) (OJ L 95, 7.4.2017, p. 1). EN Offi cial Jour nal of the European Union L 269/4                                                                                                                                           17.8.2020   (c) ‘specif ied plants’ means host plants for planting, other than seeds, belonging to the genera or species listed in Annex II 
and kno wn to be suscep tible to the specific subspecies of the specified pest. 
CHAPTER II 
ANNU AL SUR VEYS FOR THE PRESENCE OF THE SPECIFIED PEST AND CONTINGENCY PLANS 
Article 2 
Sur veys of the specif ied pest in the territor ies of the Member States 
1. Member States shall conduct annual surveys of the host plants for the detection of the specifi ed pest in their territory . 
2. Those surveys shall be carried out by the compe tent author ities, or under the official super vision of the compet ent 
author ities. 
3. Those surveys shall be performed on the basis of the level of risk. They shall take place in open air, including cropping 
fields, orchar ds, vine yards, as well as nurser ies, garden centres and/or trading centres, natural areas and other relevant 
locations. 
4. Those surveys shall consist in the collection of sam ples and testing of plants for planting. Taking into account the 
European Food Safet y Authority’s (Author ity) Guidelines for statistically sound and risk-based surveys of Xylella fastidiosa, 
the survey design and sam pling sche me used shall be able to identify within the Member State concer ned, with at least 
80 % of conf idence, a level of presence of infected plants of 1 %. 
5. Those surveys shall be carried out at appropr iate times of the year with regard to the possibility to detect the specifi ed 
pest, taking into account the biology of that pest and its vect ors, the presence and biology of host plants, and the scientifi c 
and technical information refer red to in the Author ity’s Pest Survey Card on Xylella fastidiosa. 
6. The presence of the specifi ed pest shall be monitored by one of the molecular tests listed in Annex IV. In case of 
positive results detected in areas other than the demarcate d areas, the presence of the specifi ed pest shall be conf irmed by 
one more positive molecular test listed in that Annex, targe ting differ ent parts of the genome. Those tests shall be 
performe d on the same plant sam ple or, if appropr iate for the molecular conf irmat ory test used, on the same plant extract. 
7. The identification of the subspecies of the specifi ed pest shall be carried out on each plant species that is found 
infected by the specified pest in the demarcated area concer ned. That identification shall be carried out by using the 
molecular tests listed in Section B of Annex IV. 
8. Member State s shall repor t the results of the surveys refer red to in paragraph 1 in accordance with Article 22(3) of 
Regulation (EU) 2016/2031. 
Article 3 
Contingency plans 
1. Each Member State shall establish a continge ncy plan. That continge ncy plan shall set out the actions to be take n in its 
territory concer ning: 
(a) the eradication of the specified pest, as set out in Articles 7 to 11; 
(b) the movements of specified plants within the Union, as set out in Articles 19 to 26; 
(c) the official checks to be carried out on mov ements of specifi ed plants within the Union and of host plants into the 
Union, as set out in Articles 32 and 33. EN Offi cial Jour nal of the European Union 17.8.2020                                                                                                                                           L 269/5   Each Member State shall updat e its contingenc y plan, as appropr iate, by 31 December of each year. Contingency plans 
established under Implementing Decision (EU) 2015/789 shall be updat ed by 31 December 2020. 
2. In addition to the elements refer red to in Article 25(2) of Regulation (EU) 2016/2031, the contingency plan shall 
include all of the following elements: 
(a) the minimum resources to be made available and the procedures for making those additional resources available in case 
of a conf irmed or suspected presence of the specifi ed pest ; 
(b) rules detailing the procedures for identifying the owners of the plants to be removed, for notifying the order of removal 
and for accessing private proper ties. 
CHA PTER III 
DEMAR CATED AREAS 
Article 4 
Establishment of demarcated areas 
1. Where the presence of the specif ied pest is officially conf irmed, the Member State concer ned shall without dela y 
establish a demarcat ed area. 
Where only the presence of one or more particular subspecies of the specifi ed pest is confir med, the Member State 
concer ned may demarcate an area with regar d to those subspecies only . 
Where the confir mation of the presence of a subspecies is pending, the Member State concer ned shall demarcate that area 
with regar d to the specifi ed pest and all of its possible subspecies. 
2. The demarcated area shall consist of an infected zone and a buffer zone. 
The infect ed zone shall have a radius of at least 50 m around the plant found infec ted by the specified pest. 
The buffer zone shall be of the followi ng width: 
(a) at least 2,5 km in the case of an infec ted zone established for the purpose of taking the eradication measures refer red to 
in Articles 7 to 11; 
(b) at least 5 km in the case of an infected zone established for the purpose of taking the containment measures refer red to 
in Articles 12 to 17. 
3. The Commission shall update and publish a list of the demarcate d areas established by Member States, as notifi ed 
pursuant to Article 18(6) of Regulation (EU) 2016/2031. 
Article 5 
Derogations for the establishment of demarcated areas 
1. By way of derog ation from Article 4, the buffer zone surrounding the infec ted zone established for the purpose of 
eradication may be reduced to a width of no less than 1 km, where there is a high degree of conf idence that the initial 
presence of the specifi ed pest did not result in its spread and where all of the follo wing conditions have been fulfilled: 
(a) all specifi ed plants locat ed in the infect ed zone, irrespective of their health status, have been immediate ly sam pled and 
removed; 
(b) no other plants have been found infect ed by the specif ied pest in the infect ed zone since the eradication measures have 
been taken, on the basis of official tests carried out at least once during the course of the year, taking into account the 
Author ity’s Pest Survey Card on Xylella fastidiosa; EN Offi cial Jour nal of the European Union L 269/6                                                                                                                                           17.8.2020   (c) a survey has been carried out at least once during the first year follo wing the identif ication of the specifi ed pest in a 
zone with a width of at least 2,5 km surrounding the infected zone, whic h shows that the specif ied pest has not been 
found present in that zone. The Member State concer ned shall sam ple and test the host plants located in that zone. For 
that purpose, and taking into account the Auth ority’s Guidelines for statistically sound and risk-based surveys of Xylella 
fastidiosa, the survey design and sampli ng schem e shall be able to identify with at least 90 % confidence a level of 
presence of infected plants of 1 %, taking into account that the first 400 m surrounding the infect ed plants has a 
higher risk compared to the other part of that area; 
(d) no vect ors carrying the specified pest have been detect ed in the infec ted zone and its immediate vicinity since the 
eradication measures have been taken, on the basis of tests carried out twice during the flight season of the vect or, and 
in accordance with Internati onal Standards for Phyt osanitar y Measures. Those tests shall lead to the conclusion that the 
natural spread of the specified pest is excl uded. 
2. When reducing the width of the buffer zone pursuant to paragraph 1, the Member State concer ned shall immediately 
notify the Commission and the other Member States of the justification for that reduction. 
3. By way of deroga tion from Article 4, the Member State concer ned may decide not to establish a demarcate d area 
immediately , where all of the followi ng conditions are fulfilled : 
(a) there is evidence that the specified pest was recently introduced into the area with the plants on whic h it was found, or 
that the specifi ed pest has been found in a site with physical prote ction from the vect ors of that pest ; 
(b) the results of inspection activities indicate that those plants were infected before their introduction into the area 
concer ned; 
(c) no vect ors carrying the specifi ed pest have been detect ed, on the basis of tests carried out in the vicinity of those plants. 
4. In the case referred to in paragraph 3, the Member State concer ned shall: 
(a) carry out, in the area where the presence of the specified pest was first conf irmed, an annual survey for at least 
two years to determi ne whether any other plants have been infected and whether any further measures should be taken; 
(b) notify to the Commission and the other Member States the justification for not establishing a demarcated area, and the 
outcome of the survey refer red to in point (a) as soon as it becomes available. 
Article 6 
Lifting of the demarcated areas 
1. Where, based on the surveys refer red to in Article 10, the specif ied pest has not been detect ed in a demarcat ed area 
for a period of four years, this demarcation may be lifted. In such cases, the Member State concer ned shall notify the 
Commission and other Member States. 
2. By way of derogation from paragraph 1, in the case where the Member State concer ned has reduced the buff er zone 
to a width of no less than 1 km in accordance with Article 5(1), the Member State may lift the demarcated area after 
12 months since its initial establishment, where both of the following conditions are fulfilled : 
(a) as a result of the measures taken pursuant to Article 5(1), it is concluded with a high degree of conf idence that the 
initial presence of the specified pest was an isolat ed case and no further spread occur red in the respective demarcated 
area; 
(b) as practically close to the time of lifting, official tests have been carried out within the demarcat ed area, taking into 
account the Author ity’s Pest Survey Card on Xylella fastidiosa. For that purpose, and taking into account the Author ity’s 
Guidelines for statistically sound and risk-based surveys of Xylella fastidiosa, the survey design and sampli ng sche me 
shall be able to identify with at least 95 % conf idence a level of presence of infec ted plants of 1 %. EN Offi cial Jour nal of the European Union 17.8.2020                                                                                                                                           L 269/7   3. Where a demarcat ed area is lifted pursuant to paragraph 2, the specifi ed plants locat ed in the previously established 
demarcate d area shall be subject to intensive surveys during the follo wing two years. For that purpose, and taking into 
account the Author ity’s Guidelines for statistically sound and risk-based surveys of Xylella fastidiosa, the survey design and 
sampli ng sche me shall be able to identify with at least 80 % conf idence a level of presence of infected plants of 1 %. 
4. When lifting the demarcated area after 12 months since its initial identif ication, the Member State concer ned shall 
immediately notify the Commission and the other Member States of the justification for that lifting. 
CHA PTER IV 
ERADIC ATION MEASURES 
Article 7 
Remo val of plants 
1. The Member State concer ned shall immediately remo ve from the infect ed zone: 
(a) plants known to be infected by the specifi ed pest ; 
(b) plants showi ng symp toms indicating possible infec tion by that pest or suspected to be infected by that pest ; 
(c) plants which belong to the same species as that of the infect ed plant, regardless of their health status; 
(d) plants of other species than that of the infect ed plant, which have been found infected in other parts of the demarcate d 
area; 
(e) specifi ed plants, other than the ones refer red to in points (c) and (d), which have not been immediat ely subjected to 
sampling and molecular testing and have not been found free from the specified pest. 
2. When remo ving the plants refer red to in paragraph 1, the Member State concer ned shall take all necessar y 
precautions into account, and shall organise the removal on the basis of the level of risk posed by those plants 
3. By way of derogat ion from points (b), (c) and (d) of paragraph 1, Member State s may decide that individual specified 
plants officiall y designat ed as plants with histo ric value do not need to be removed, provid ed that all of the follo wing 
conditions have been fulfilled: 
(a) the specified plants concer ned are subject to annual inspection, sampli ng and testing by one of the molecular tests 
listed in Annex IV and it is conf irmed that they are not infected by the specifi ed pest ; 
(b) the individual specified plants or the area concer ned are subject to appropr iate phytosanitar y treatments against the 
vector population of the specified pest, in all its stages . Those treatments may include chemical, biological or 
mechanical methods, taking into account the local conditions. 
Article 8 
Measures against the vecto rs of the specif ied pest 
1. The Member State concer ned shall apply appropr iate phytosan itary treatments, in the infect ed zone, against the 
vect or population of the specifi ed pest, in all its stages. It shall apply those treatments prior to and during the removal of 
the plants referred to in Article 7(1). Those treatments shall include efficient chemical, biological or mechanical treatments 
against the vect ors, taking into account the local conditions. 
2. The Member State concer ned shall apply agricultural practices for the control of the vect or population of the 
specifi ed pest, in all its stages, in the infected zone and in the buff er zone. It shall apply those practices at the most 
appropr iate time of the year, regar dless of the remo val of the plants concer ned. Those practices shall include efficient 
chemical, biological or mech anical treatments against the vect ors, as appropr iate, taking into account the local conditions. EN Offi cial Jour nal of the European Union L 269/8                                                                                                                                           17.8.2020   Article 9 
Dest ruction of plants 
1. The Member State concer ned shall destro y the plants and parts of plants refer red to in Article 7(1), in a manner 
ensur ing that the specifi ed pest is not spread, in situ or in a nearby location designat ed for this purpose within the infect ed 
zone or, provided that those plants or parts of plants are covered by net against the vect ors, at the shor test distance from 
that location. 
2. The Member State concer ned may decide, on the basis of the risk level, to limit the destr uction to the branches and 
foliag e only and subject the relat ed wood to phytosanitar y treatment as refer red to in Article 8(1). The root syste m of those 
plants shall be either remo ved or devitalised, with an appropr iate phytosanitar y treatment to avoid resprouting. 
Article 10 
Annual surveillance of the demarcated area 
In the entire demarcated area, the Member State concer ned shall monitor , at the most appropr iate times, the presence of the 
specifi ed pest by annual surveys, in accordance with Article 2(5) and (6), and taking into account the information refer red 
to in the Author ity’s Pest Survey Card on Xylella fastidiosa. 
In the infected zones, the Member State concer ned shall sample and test the host plants, including the specifi ed plants 
which have not been removed pursuant to Article 7(1). For that purpose, and taking into account the Author ity’s 
Guidelines for statistically sound and risk-based surveys of Xylella fastidiosa, the survey design and sampling scheme shall 
be able to identify with at least 90 % conf idence a level of presence of infected plants of 0,5 %. 
In buffe r zones, the Member State concer ned shall sample and test the host plants, as well as other plants showing 
symp toms indicating possible infection or suspect ed to be infec ted by that pest. For that purpose, and taking into account 
the Author ity’s Guidelines for statistically sound and risk-based surveys of Xylella fastidiosa, the survey design and samp ling 
schem e shall be able to identify with at least 90 % conf idence a level of presence of infec ted plants of 1 %, taking into 
account that the first 400 m surrounding the infected zones has a higher risk. 
The Member State concer ned shall also monitor the presence of the specifi ed pest in the vect ors locat ed in the demarcate d 
area in order to determine the risk of further spreading posed by vectors and to evaluate the effectiveness of the 
phytosanitar y control measures applied in accordance with Article 8. 
Article 11 
Other relevant measures for the eradication of the specif ied pest 
1. The Member State concer ned shall take any other measure which may contr ibute to the eradication of the specifi ed 
pest, in accordance with International Standard for Phytosanitar y Measure (‘ISPM’) No 9(9) and to contr ibuting to applying 
an integrat ed approac h in accordance with the principles set out in ISPM No 14(10). 
2. The Member State concer ned shall take measures addressing any particular ity or complication that could reasonably 
be expect ed to prevent, hinder or dela y eradication, in particular those related to the adequate destr uction of all plants that 
are infected or suspect ed of infection, or to the accessibility of their location, public or private ownership or the person or 
entity responsible for them. 
(9)Guidelines for pest eradication programmes – Reference Standard ISPM No 9 by the Secretar iat of the International Plant Protection 
Con vention, Rome. Published 15 December 2011. 
(10)The use of integrat ed measures in a systems approac h for pest risk management – Reference Standard ISPM No 14 by the Secretar iat 
of the Intern ational Plant Protection Con vention, Rome. Published 8 Januar y 2014. EN Offi cial Jour nal of the European Union 17.8.2020                                                                                                                                           L 269/9   3. The Member State concer ned shall carry out appropr iate investigations to identify the origin of the infection. It shall 
trace the host plants associated with the case of infec tion concer ned, including those which were mov ed before a 
demarcate d area was established. The results of such investigations shall be communicat ed to the Commission and the 
Member States in which those plants concer ned originat e, to the Member States through which those plants have mov ed 
and to the Member States where those plants have move d into. 
CHA PTER V 
CONT AINMENT MEASURES 
Article 12 
General provisions 
The compet ent author ity of the Member State concer ned may decide to apply the containment measures set out in Articles 
13 to 17, inste ad of eradication measures, in an infected zone listed in Annex III. 
Article 13 
Remo val of plants in an infected zone listed in Annex III 
1. The Member State concer ned shall remove all plants which have been found to be infected by the specified pest on the 
basis of the monitori ng refer red to in Article 15(2). 
That removal shall take place immediately after the official identif ication of the presence of the specifi ed pest or, if the 
specifi ed pest is detected outside the flight season of the vect or, that remo val shall take place before the next flight season 
of the vect or. All necessar y precautions shall be taken to avoid spreading of the specified pest and its vect ors during and 
after removal. 
2. By way of derogation from paragraph 1, the Member State concer ned may decide, for scientific purposes, not to 
remo ve plants whic h have been found to be infec ted by the specifi ed pest in the sites of plants with particular cultural and 
social value, referred to in Article 15(2)(b). 
Article 14 
Measures agains t the vectors of the specif ied pest in the infected zones listed in Annex III 
1. The Member State concer ned shall apply appropr iate phytosanitar y treatments against the vect or population of the 
specifi ed pest, in all its stage s, on the plants referred to in Article 13(1) prior to their remo val and around the plants 
refer red to in Article 13(2). Those treatments shall include efficient chemical, biological or mecha nical treatments against 
the vect ors, taking into account the local conditions. 
2. In the areas referred to in points (a) and (b) of Article 15(2), the Member State concer ned shall apply agricultural 
practices for the control of the vect or population of the specified pest, in all its stages, at the most appropr iate time of each 
year. Those practices shall include efficient chemical, biological or mechanical treatments against the vect ors, taking into 
account the local conditions. 
Article 15 
Annual surveillance of the infected zones listed in Annex III 
1. The Member State concer ned shall, at least in the parts of the infected zone refer red to in paragraph 2, immediate ly 
sample and test, within a radius of 50 m around the plants whic h have been found to be infected by the specifi ed pest, the 
follo wing plants: 
(a) all specifi ed plants whic h belong to the species of the specif ied plants which have been found infected in the same 
demarcated area; and EN Offi cial Jour nal of the European Union L 269/10                                                                                                                                         17.8.2020   (b) all other plants showi ng symp toms indicating possible infec tion by that pest or suspected to be infected by that pest. 
2. The Member State concer ned shall monitor , at the most appropr iate times, the presence of the specified pest by 
annual surveys, taking into account the information refer red to in the Auth ority’s Pest Survey Card on Xylella fastidiosa. 
That monitor ing shall take place at least in the followi ng parts of the infect ed zone listed in Annex III: 
(a) within an area measur ing at least 5 km from the border of the infected zone with the buff er zone; 
(b) in the proximity of the sites of plants with particular cultural and social value locat ed outside the area refer red to in 
point (a) and designated according ly by the Member State. 
In those parts of the infec ted zone, the Member State concer ned shall carry out sampling and testin g of the species of host 
plants found infected in the demarcat ed area, in accordance with Article 2(6). For that purpose, taking into account the 
Authority’s Guidelines for statistically sound and risk-based surveys of Xylella fastidiosa, the survey design and sampling 
schem e shall be able to identify with at least 90 % conf idence a level of presence of infec ted plants of 0,7 %. It shall also 
sample and test the vect or population for the presence of the specifi ed pest. 
3. Point (a) of paragraph 2 shall not apply in the case of islands whic h are entirely under containment measures and are 
situated more than 5 km to the nearest Union land territory. 
4. In buffer zones, the Member State concer ned shall sample and test the host plants, as well as other plants showing 
symp toms indicating possible infection by that pest or suspect ed to be infected by that pest. For that purpose, taking into 
account the Author ity’s Guidelines for statistically sound and risk-based surveys of Xylella fastidiosa, the survey design and 
sampli ng scheme shall be able to identify with at least 90 % conf idence a level of presence of infected plants of 1 %, taking 
into account that the first 400 m adjacent to the infec ted zones pose a higher risk. 
5. The Member State shall monitor the presence of the specified pests in vectors in the parts of the infected zone refer red 
to in paragraph 2 and in the buffer zone in order to deter mine the risk of further spreading posed by vect ors and to evaluate 
the effectiveness of the phytosan itary control measures applied in accordance with Article 14. 
Article 16 
Dest ruction of plants 
1. The Member State concer ned shall, in situ or in a nearby location designate d for this purpose within the infected zone 
liste d in Annex III, destro y plants and parts of plants whic h have been found to be infected by the specifi ed pest, in a 
manner ensur ing that the specified pest is not spread. 
2. The Member State concer ned may decide to limit the destr uction to branch es and foliage only and subject the related 
wood to appropr iate phytosan itary treatments in accordance with Article 14(1), where it concludes that those plants do not 
pose any risk for further spreading of the specifi ed pest. The root system of those plants shall be either remo ved or 
devitalised with an appropr iate phyt osanitar y treatment to avoid resprouting. 
Article 17 
Other relevant measures for the containment of the specif ied pest 
The Member State concer ned shall take measures addressing any particular ity or compl ication that could reasonably be 
expecte d to prevent, hinder or dela y containment, in particular those related to the adequate destr uction of all plants that 
are infected or suspect ed of infection, or to the accessibility of their location, public or private ownership or the person or 
entity responsible for them. EN Offi cial Jour nal of the European Union 17.8.2020                                                                                                                                         L 269/11   CHA PTER VI 
PLANTING OF SPECIFIED PLANTS IN INFECTED ZONES 
Article 18 
Author isation concer ning the planting of specif ied plants in infected zones 
The planting of specified plants in infect ed zones may only be author ised by the Member State concer ned in one of the 
follo wing cases: 
(a) those specifi ed plants are grown in insect-proof sites of production free from the specifi ed pest and its vect ors; 
(b) those specified plants prefe rably belong to varieties assessed as being resistant or tolerant to the specifi ed pest and are 
planted in the infect ed zones listed in Annex III, but outside the area refer red to in Article 15(2)(a); 
(c) those specifi ed plants belong to the same species of plants which have been tested and found free from the specified 
pest on the basis of the survey activities carried out for at least the past two years in accordance with Article 10 and 
are plante d in the infec ted zones established for the purpose of eradication. 
CHAPTER VII 
MO VEMENT WITHIN THE UNION OF SPECIFIED PLANTS 
Article 19 
Movement out of a demarcated area, and from the respectiv e infected zones into the buffer zones, of specif ied 
plants which hav e been grown in author ised production sites located in that demarcated area 
The move ment out of a demarcat ed area, and from the respective infect ed zones into the buffe r zones, of specifi ed plants 
which have been grown in a production site locat ed in that demarcated area, may only be allowed where all of the 
follo wing conditions are fulfilled: 
(a) the specified plants have been grown during their entire production cycle in a site that has been author ised in 
accordance with Article 24 or have been present in such a site for at least the last three years; 
(b) throughout the time of growth of the specifi ed plants, neither the presence of the specified pest nor that of its vect ors 
were found in the site; 
(c) the specified plants are subject to phytosan itary treatments against the vect or population, in all its stages, at appropr iate 
times of the year to maintain freedom from vect ors of the specified pest. Those treatments shall include, as appropr iate, 
efficient chemical, biological or mech anical methods, taking into account the local conditions; 
(d) the specified plants are transpor ted through or within the demarcat ed area in closed containers or packaging, ensur ing 
that infection with the specifi ed pest or any of its vect ors cannot occur; 
(e) as close as possible to the time of move ment, the specified plants have been subjected to molecular testing for the 
presence of the specifi ed pest on the basis of a test listed in Annex IV, using a sam pling scheme able to identify with at 
least 80 % conf idence a level of presence of infected plants of 1 %. EN Offi cial Jour nal of the European Union L 269/12                                                                                                                                         17.8.2020   Article 20 
Movement out of a demarcated area, and from the respectiv e infected zones into the buffer zones, of specif ied 
plants which have nev er been found infected in that demarcated area 
The movement out of a demarcate d area, and from the respective infected zone into the buffer zones, of specifi ed plants 
which have never been found infected in that demarcat ed area, may only be allowed where all of the following conditions 
are fulfilled : 
(a) the specified plants have been grown in a site that belongs to a profes sional operator register ed in accordance with 
Article 65 of Regulation (EU) 2016/2031; 
(b) the specified plants belong to species of plants whic h have been grown for at least part of their life in a demarcat ed area 
and have been subjected, during three years from the establishment of the demarcate d area, to survey activities as 
refer red to in Articles 10 and 15 and never found to be infect ed with the specifi ed pest ; 
(c) the species of the specifi ed plants referred to in point (b) are published in the Commission database of host plants 
which are not known to be infected in that specific demarcate d area; 
(d) the specified plants are subject to phytosan itary treatments against the vect or population, in all its stages, at appropr iate 
times of the year to maintain freedom from vect ors of the specified pest. Those treatments shall include, as appropr iate, 
efficient chemical, biological or mech anical methods based on local conditions; 
(e) as practically close to the time of mov ement as possible, the lots of the specified plants were subjected to inspection and 
molecular testing by the compet ent author ity, using a sampling scheme able to identify with at least 95 % confidence a 
level of presence of infected plants of 1 %; 
(f) as practically close to the time of mov ement as possible, the lots of the specified plants were subjected to phytosan itary 
treatments against all vect ors of the specified pest. 
Article 21 
Movement out of a demarcated area, and from the respectiv e infected zones into the buffer zones, of specif ied 
plants which have been grown for the entire production cycle in vitro in that demarcated area 
The move ment out of a demarcat ed area, and from the respective infect ed zones into the buffe r zones, of specifi ed plants 
which have been grown for the entire production cycle in vitro in that demarcat ed area, may only be allowed where all of 
the follo wing conditions are fulfilled: 
(a) the specified plants have been grown for their entire production cycle in a site that has been author ised in accordance 
with Article 24; 
(b) the specifi ed plants have been grown in a transparent container under sterile conditions and fulfil one of the followi ng 
conditions: 
(i) they have been grown from seeds; 
(ii) they have been propagat ed, under sterile conditions, from mother plants which have spent their entire lives in an 
area of the Union territory free from the specified pest and which have been tested and found free from the 
specifi ed pest ; 
(iii) they have been propagat ed, under sterile conditions, from mother plants which have been grown in a site fulfilling 
the conditions laid down in Article 19 and whic h have been tested and found free from the specifi ed pest using a 
sampling schem e able to identify with at least 95 % conf idence a level of presence of infected plants of 1 %; EN Offi cial Jour nal of the European Union 17.8.2020                                                                                                                                         L 269/13   (c) the specifi ed plants are transpor ted through or within demarcat ed areas in a container under steril e conditions that 
precludes the possibility of infection by the specified pest through its vect ors. 
Article 22 
Movement out of a demarcated area, and from the respectiv e infected zones into the buffer zones, of dor mant 
plants of Vitis which have been grown for part of their life in that demarcated area 
Movement out of a demarcated area, and from the respective infected zones into the buffe r zones, of dormant plants of Vitis 
intended for planting, other than seeds, which have been grown for part of their life in that demarcated area and are listed as 
specifi ed plants for that demarcat ed area, may only be allowed where all of the following conditions are fulfilled: 
(a) the plants have been grown in a site that belongs to an operat or registere d in accordance with Article 65 of Regulation 
(EU) 2016/2031; 
(b) as practically close to the time of movement as possible, the plants have undergone an appropr iate ther motherap y 
treatment in a treatment facility author ised and super vised by the compet ent author ity for that purpose, whereby the 
dormant plants are submerged for 45 minutes in water heat ed to 50 °C. 
Article 23 
Movement within the infected zones, within the buffer zones, and from the buffer zones into their respectiv e 
infected zones, of specif ied plants which have been grown for part of their life in a demarcated area 
The mov ement within the infec ted zones, within the buff er zones, and from the buffe r zones into their respective infected 
zones, of specified plants whic h have been grown for at least part of their life in a demarcat ed area may only be allowed 
where all of the follo wing conditions are fulfilled: 
(a) the specifi ed plants have been grown in a site that belongs to an operat or register ed in accordance with Article 65 of 
Regulation (EU) 2016/2031; 
(b) that site is subject to annual sampling and testing by the compet ent author ity for the presence of the specified pest, 
taking into account the information set out in the Auth ority’s Pest Survey Card on Xylella fastidiosa; 
(c) the results of the annual inspection and of the testing of a representative sample conf irm the absence of the specif ied 
pest ; 
(d) the specified plants are subject to phytosan itary treatments against the vect or population, in all its stages, at appropr iate 
times of the year to maintain freedom from vect ors of the specified pest. Those treatments shall include, as appropr iate, 
efficient chemical, biological or mech anical methods, taking into account the local conditions; 
(e) the professional operators shall ask the person receiving those plants to sign a declaration that those plants are not 
going to be mov ed out of those zones. 
Article 24 
Author isation of production sites 
1. The compet ent author ity may only author ise a production site for the purposes of Articles 19 and 21 where it fulfils 
all of the followi ng conditions: 
(a) it is registere d in accordance with Article 65 of Regulation (EU) 2016/2031; 
(b) it has been author ised by the compet ent author ity as a site physically protect ed against the specified pest and its vect ors; 
(c) it has been subjecte d annually to at least two inspections by the compet ent author ity, at the most appropr iate time. EN Offi cial Jour nal of the European Union L 269/14                                                                                                                                         17.8.2020   2. Where, during the annual inspections, the compet ent author ities detect the presence of the specifi ed pest, or damag es 
to the physical protection refer red to in point (b) of paragraph 1, they shall immediate ly revok e the author isation of the site 
and temporar ily suspend the mov ement of the specifi ed plants out of the demarcated areas concer ned, and from the 
respective infected zones into the buff er zones. 
3. Each Member State shall establish and updat e a list of all sites author ised in accordance with paragraph 1. 
It shall transmit that list to the Commission and the other Member States immediately after establishing or updating that 
list. 
Article 25 
Movement within the Union of specif ied plants which have nev er been grown inside a demarcated area 
1. Specified plants which have never been grown inside a demarcated area may only be move d within the Union where 
they have been grown in a site that fulfils the followi ng conditions: 
(a) it belongs to a professional operat or registered in accordance with Article 65 of Regulation (EU) 2016/2031 and is 
subject to annual inspection by the compet ent author ity; 
(b) it is subject, as appropr iate for the level of risk, to sampli ng and testing for the presence of the specifi ed pest, using a 
test listed in Annex IV and taking into account the information set out in the Authority’s Pest Survey Card on Xylella 
fastidiosa. 
2. By way of derog ation from paragraph 1, plants for planting, other than seeds, of Coffea, Lava ndula dentata L., Nerium 
oleander L., Olea europaea L., Polygala myrtifolia L. and Prunus dulcis (Mill.) D.A . Webb, may only be mov ed for the first time 
within the Union where the follo wing conditions are fulfilled: 
(a) they have been grown in a site that is subject to annual inspection by the compet ent author ity; 
(b) that site is subject to sam pling and testing for the presence of the specified pest, taking into account the information set 
out in the Author ity Pest Survey Card on Xylella fastidiosa and using a sampling sche me able to identify with at least 
80 % conf idence a level of presence of infect ed plants of 1 %. 
Article 26 
Movement within the Union of pre-basic mother plants or pre-basic mater ial, which have been grown outside a 
demarcated area 
Pre-basic mother plants as defined in Article 1(3) of Commission Implementing Directive 2014/98/EU (11) or pre-basic 
mater ial as defined in Article 2(5) of Council Directive 2008/90/EC (12) which belong to the species Juglans regia L., Olea 
europ aea L., Prunus amygdalus Batsch, P. amygdalus x P. persica, P. armeniaca L., P. avium (L.) L., P. cerasus L., P. domestica L., P. 
domestica x P. salicina, P. dulcis (Mill.) D.A . Webb, P. persica (L.) Batsch, and P. salicina Lindley and which have been grown 
outside a demarcated area and spent at least part of their life outside insect-proof facilities, may only be mov ed within the 
Union where they are accompan ied by a plant passpor t and the following conditions have been fulfilled: 
(a) they have been certified in accordance with Article 1 of Commission Implementing Decision (EU) 2017/925 (13); 
(11)Commission Implementing Directive 2014/98/EU of 15 October 2014 impl ementing Council Directive 2008/90/EC as regar ds 
specific requirements for the genus and species of fruit plants referred to in Annex I thereto, specific requirements to be met by 
suppliers and detailed rules concer ning official inspections, OJ L 298, 16.10.2014, p. 22. 
(12)Council Directive 2008/90/EC of 29 Sept ember 2008 on the marketing of fruit plant propagatin g material and fruit plants intended 
for fruit production, OJ L 267, 8.10.2008, p. 8. 
(13)Commission Implementing Decision (EU) 2017/925 of 29 May 2017 temporar ily author ising certain Member States to certify pre- 
basic mat erial of certain species of fruit plants, produced in the field under non-insect proof conditions, and repealing Implementing 
Decision (EU) 2017/167, OJ L 140, 31.5.2017, p. 7. EN Offi cial Jour nal of the European Union 17.8.2020                                                                                                                                         L 269/15   (b) within the shor test possible time prior to their move ment, they have been subjected to visual inspection, sampli ng and 
molecular testing for the presence of the specifi ed pest carried out in accordance with Intern ational Standards for 
Phyt osanitar y Measures. 
Article 27 
Plant passpor ts 
The plants referred to in Articles 19 to 26 shall only be moved within the Union if accompan ied by a plant passpor t, subject 
to the requirements of Articles 78 to 95 of Regulation (EU) 2016/2031. 
In the case of the specifi ed plants refer red to in Article 23, the followi ng additional conditions shall apply : 
(a) in the case where they mov e within the infected zones only , the indication ‘Infect ed Zone – XYLEF A’ shall be included 
next to the traceability code refer red to in paragraph 1(e) of Part A of Annex VII to Regulation (EU) 2016/2031; 
(b) in the case where they mov e within the buffe r zone, or from the buffer zone into the infect ed zone, the indication 
‘Buffer Zone and Infected Zone – XYLEF A’ shall be included next to the traceability code refer red to in paragraph 1 (e) 
of Part A of Annex VII to Regulation (EU) 2016/2031. 
CHA PTER VIII 
INTR ODUCTION INTO THE UNION OF HOST PLANTS 
Article 28 
Introduction into the Union of host plants originating in a third countr y where the specif ied pest is kno wn not to 
be present 
Host plants originating in a third countr y where the specifi ed pest is known not to be present, may only be introduced into 
the Union where the followi ng conditions are fulfilled: 
(a) the national plant prot ection organisation of the third countr y concer ned has communicate d in writing to the 
Commission that the specified pest is kno wn not to be present in the countr y on the basis of inspection, samp ling and 
molecular testing by the compet ent author ity, using a test listed in Annex IV, and in accordance with relevant 
Intern ational Standards for Phytosan itary Measures. Taking into account the Author ity’s Guidelines for statistically 
sound and risk-based surveys of Xylella fastidiosa, the survey design and sam pling schem e used are able to identify with 
at least 80 % confidence a level of presence of infected plants at national level of 1 %; 
(b) the host plants are accompan ied by a phytosanitar y certificate stating under the rubric ‘Additional Declaration’ that the 
specifi ed pest is not present in the countr y; 
(c) host plants have been grown in a site that is subject to annual inspection by the compet ent author ity and, as 
appropr iate for the level of risk, with sam pling and testing, listed in Annex IV, carried out at the appropr iate times on 
those plants for the presence of the specif ied pest ; 
(d) plants for planting, other than seeds, of Coffea, Lava ndula dentata L., Nerium oleander L., Olea europaea L., Polygala 
myrtifolia L. and Prunus dulcis (Mill.) D.A. Webb, have been grown in a site that is subject to annual inspection by the 
compet ent author ity, with sampling and testing, liste d in Annex IV, carried out at the appropr iate times on those 
plants for the presence of the specified pest, using a sam pling scheme able to identify with at least 80 % conf idence a 
level of presence of infected plants of 1 %; 
(e) on entr y into the Union, the host plants have been check ed by the compet ent author ity in accordance with Article 33 
and the presence of the specifi ed pest has not been found. EN Offi cial Jour nal of the European Union L 269/16                                                                                                                                         17.8.2020   Article 29 
Introduction into the Union of host plants originating in a pest free area of an infected countr y 
Host plants originating in a third countr y where the specified pest is known to be present may only be introduced into the 
Union where all of the following conditions are fulfilled : 
(a) the host plants originate in an area whic h has been declared to be free from the specified pest, by the national plant 
protection organisation concer ned in accordance with the relevant Intern ational Standards for Phytosanitar y Measures 
and on the basis of official surveys based on sampling and testing, using a test liste d in Annex IV. Taking into account 
the Auth ority’s Guidelines for statistically sound and risk-based surveys of Xylella fastidiosa, the survey design and 
sampling scheme used are able to identify with at least 80 % confidence a level of presence of infected plants of 1 %; 
(b) the national plant prot ection organisation of the third countr y concer ned has communicate d in writing to the 
Commission the name of that area; 
(c) the host plants are accompan ied by a phytosan itary certificate stating under the rubric ‘place of origin’ that the host 
plants concer ned have spent their entire life in the area referred to in point (a), with specifi c reference to the name of 
that area; 
(d) host plants have been grown in a site that is subject to annual inspection by the compet ent author ity and, as 
appropr iate for the level of risk, with sam pling and testing, listed in Annex IV, carried out at the appropr iate times on 
those plants for the presence of the specif ied pest ; 
(e) plants for planting, other than seeds, of Coffea, Lava ndula dentata L., Nerium oleander L., Olea europaea L., Polygala 
myrtifolia L. and Prunus dulcis (Mill.) D.A. Webb, have been grown in a site that is subject to annual inspection by the 
compet ent author ity, with sampling and testing, liste d in Annex IV, carried out at the appropr iate times on those 
plants for the presence of the specified pest, using a sam pling scheme able to identify with at least 80 % conf idence a 
level of presence of infected plants of 1 %; 
(f) on entr y into the Union, the host plants have been check ed by the compet ent author ity in accordance with Article 33 
and the presence of the specifi ed pest has not been found. 
Article 30 
Introduction into the Union of host plants originating in a pest free production site of an infected countr y 
1. Host plants originating in a third countr y where the specified pest is kno wn to be present may only be introduced 
into the Union where the follo wing conditions are fulfilled: 
(a) the host plants originate in a production site that has been author ised as pest free by the national plant prot ection 
organisation in accordance with Article 31; 
(b) the national plant prot ection organisation of the third countr y concer ned has communicate d in writing to the 
Commission the list of pest free production sites, including their location within the countr y; 
(c) the host plants are accompan ied by a phytosan itary certificate stating the followi ng: 
(i) under the rubric ‘additional declaration’, that the host plants have been produced for their entire production cycle 
in one or more sites author ised as pest free by the national plant prote ction organisation in accordance with 
Article 31 and that the host plants have been transpor ted in closed containers or packag ing, ensur ing that 
infection with the specifi ed pest through its vect ors cannot occur ; 
(ii) under the rubric ‘place of origin’ , the name or code of the pest free production site(s); 
(d) on entr y into the Union, the host plants have been check ed by the compet ent author ity in accordance with Article 33 
and the presence of the specifi ed pest has not been found. EN Offi cial Jour nal of the European Union 17.8.2020                                                                                                                                         L 269/17   2. Host plants which originate in a third countr y where the specifi ed pest is known to be present and have been grown 
for the entire production cycle in vitro, may only be introduced into the Union where the following conditions are fulfilled: 
(a) the host plants meet one of the following conditions: 
(i) they have been grown from seeds; 
(ii) they have been propagat ed, under sterile conditions, from mother plants which have spent their entire lives in an 
area free from the specifi ed pest and which have been tested and found free from the specified pest ; 
(iii) they have been propagat ed, under sterile conditions, from mother plants which have been grown in a site fulfilling 
the conditions of Article 31 and which have been tested and found free from the specifi ed pest ; 
(b) the host plants have been grown in a production site that has been author ised as pest free by the national plant 
protection organisation in accordance with Article 31; 
(c) the national plant prot ection organisation of the third countr y concer ned has communicate d in writing to the 
Commission the list of pest free production sites, including their location within the countr y.; 
(d) the host plants are accompan ied by a phytosan itary certificate stating the followi ng: 
(i) under the rubric ‘additional declaration’, that the host plants have been produced in vitro for their entire production 
cycle in one or more sites author ised as pest free by the national plant prot ection organisation in accordance with 
Article 31 and that the host plants have been transpor ted in closed containers or packag ing, ensur ing that 
infection with the specifi ed pest or any of its known vect ors cannot occur ; 
(ii) under the rubric ‘place of origin’ , the name or code of the pest free production site. 
Article 31 
Author isation of production sites as pest free 
A production site may only be author ised as pest free where all of the followi ng conditions are fulfilled: 
(a) the production site has been declared by the national plant prote ction organisation as an insect-proof site free from the 
specifi ed pest and its vect ors, in accordance with the relevant Internati onal Standards for Phytosanitar y Measures; 
(b) the production site has undergone phyt osanitar y treatments against the vect or population, in all its stages, at 
appropr iate times of the year to maintain freedom from vect ors of the specified pest. Those treatments shall include 
efficient chemical, biological or mech anical methods based on local conditions; 
(c) the production site is subjected annually to at least two inspections by the compet ent author ity, at the most appropr iate 
time; 
(d) as close as possible to the time of mov ement, the host plants originating in the production site have been subjected to 
molecular testing for the presence of the specifi ed pest using a test listed in Annex IV and using a samp ling scheme 
able to identify with at least 90 % conf idence a level of presence of infected plants of 1 %. 
Where during the annual inspections, the compet ent author ities detect the presence of the specified pest, or damages 
compromisi ng the insect-proof conditions of the pest free production site, they shall immediately revok e the author isation 
of the site and temporar ily suspend the movement of the host plants. They shall immediately inform the Commission 
thereof. EN Offi cial Jour nal of the European Union L 269/18                                                                                                                                         17.8.2020   CHA PTER IX 
OFFICIAL CHEC KS ON MO VEMENT OF SPECIFIED PLANTS WITHIN , AND OF HOST PLANTS INTO, THE UNION 
Article 32 
Official checks on movements of specif ied plants within the Union 
1. Member States shall carry out systema tic official checks on specifi ed plants being mov ed out of a demarcat ed area, or 
from an infect ed zone to a buff er zone. 
2. Such checks shall be perf ormed at least in the locations, including roads, airports and ports, where the host plants are 
mov ed from infected zones into buff er zones or other parts of the Union territory . 
3. Those chec ks shall include a documentar y check, and an identity chec k of the specified plants. 
4. Those chec ks shall be carried out irrespective of the declared origin of the specified plants, ownership or the person 
or entity responsible for them. 
5. Where those chec ks show that the conditions laid down in Articles 19 to 23 are not satisfied, the Member State whic h 
carried them out shall immediate ly destro y the non-compliant plant in situ or in a nearb y location. That action shall be 
carried out taking all necessar y precautions to avoid spreading of the specif ied pest, and any vect ors carried by that plant, 
during and after remo val. 
Article 33 
Official check s at introduction into the Union 
1. All consignments of host plants introduced into the Union from a third countr y shall be officially chec ked at the 
point of entr y into the Union or at the place of destination established in accordance with Commission Directive 
2004/103/EC (14). 
2. Ιn case of host plants originating in areas where the specif ied pest is known to occur , the compet ent author ity shall 
carry out an inspection, consisting of sam pling and testing of the lot of the specified plants to confir m the absence of the 
specifi ed pest, using a sam pling schem e able to identify with at least 80 % conf idence, a level of infec ted plants of 1 %, 
taking account of ISPM No 31. 
3. Paragraph 2 shall not apply to host plants whic h have been grown for the entire production cycle in vitro and are 
transpor ted in transparent containers under sterile conditions. 
CHAPTER X 
COMMUNIC ATION ACTIVITIES 
Article 34 
Awareness campaigns 
1. Member States shall mak e information available to the general public, traveller s, profess ional and intern ational 
transpor t operat ors concer ning the threat of the specified pest for the Union territory. They shall make that information 
publicly available, in the form of target ed awareness campaigns on the respective website s of the compet ent author ity or 
other websites designate d by it. 
(14)Commission Directive 2004/103/EC of 7 Octo ber 2004 on identity and plant health checks of plants, plant products or other objects, 
listed in Part B of Annex V to Council Directive 2000/29/EC, whic h may be carried out at a place other than the point of entr y into the 
Community or at a place close by and specifying the conditions relate d to these chec ks (OJ L 313, 12.10.2004, p. 16). EN Offi cial Jour nal of the European Union 17.8.2020                                                                                                                                         L 269/19   2. Within the demarcate d areas, the Member State concer ned shall raise public awareness concer ning the threat of the 
specifi ed pest and the measures adopt ed to prevent its introduction into and spread within the Union. It shall ensure that 
the general public, travellers and relevant operat ors are aware of the delimitation of the demarcate d area, the infected zone 
and the buffe r zone. The Member State concer ned shall also inform relevant operators about the measures to take against 
the vect or as laid down in Articles 8 and 14. 
CHA PTER XI 
FINAL PROVISIONS 
Article 35 
Repor ting on measures by Member States 
1. Member States shall, by 30 Apr il of each year, transmit to the Commission and to the other Member States a repor t 
on the measures taken during the preceding year, pursuant to Articles 2, 4, 5, 7 to 18 and 32, as applicable, and on the 
results of those measures. 
The results of the surveys carried out pursuant to Articles 10 and 15 in demarcated areas shall be transmitted to the 
Commission using the templat es referred to in Annex V. 
2. Member States shall, by 31 December of each year , transmit to the Commission and the other Member States a plan 
setting out the measures to be taken pursuant to Articles 2, 4, 5, 7 to 18 and 32, as applicable, in the followi ng year . That 
plan shall set out the scheduled time period of each measure, the deadlines for implementing the measures and the 
allocat ed budget for all measures. 
3. Where justif ied by the development of the respective phytosan itary risk, Member States shall adapt the respective 
measures and according ly update the plan refer red to paragraph 2. They shall immediately communicate that update to the 
Commission and the other Member States. 
4. The Member State concer ned shall immediately notify the Commission and the other Member States of any official 
identif ication of the presence of the specifi ed pest in the locations refer red to in Article 15(2)(a). 
Article 36 
Compliance 
Member States shall, as necessar y in order to compl y with this Regulation, repeal or amend the measures, which they have 
adopt ed to prote ct themselves against the introduction and spread of the specifi ed pest. They shall immediately inform the 
Commission of the repeal or amendment of those measures. 
Article 37 
Repeal 
Implementing Decision (EU) 2015/789 is repealed. 
Article 38 
Entr y into force and application 
This Regulation shall enter into force on the third day following that of its publication in the Official Journal of the European 
Union. EN Offi cial Jour nal of the European Union L 269/20                                                                                                                                         17.8.2020   However , the second senten ce of Article 2(4), the third sente nce of Article 5(1)(c), the second senten ce of Article 28(a) and 
the second sentence of Article 29(a) shall apply from 1 Januar y 2023. 
This Regulation shall be binding in its entirety and directly applicable in all Member States. 
Done at Brussels, 14 August 2020.  
For the Commission 
The President 
Ursula VON DER LEYEN     EN Offi cial Jour nal of the European Union 17.8.2020                                                                                                                                         L 269/21   ANNEX 
‘ANNEX I 
List of plants kno wn to be susceptible to one or more subspecies of the specif ied pest (‘host plants’) 
Acaci a 
Acer 
Albizia julibr issin Durazz. 
Alnus rhombif olia Nutt. 
Amar anthus retroflexus L. 
Ambro sia 
Ampelopsis arbor ea (L.) Koehne 
Ampelopsis brevipedunculata (Maxim.) Trautv . 
Ampelopsis cordata Michx. 
Anthyllis hermanniae L. 
Artemisia 
Aspar agus acutif olius L. 
Bacchar is 
Brassica 
Calicotome spinosa (L.) Link 
Calicotome villosa (Poiret) Link 
Callicar pa amer icana L. 
Callistemon citrinus (Cur tis) Skee ls 
Calluna vulgaris (L.) Hull 
Carya 
Cathar anthus 
Celtis occidentalis L. 
Cerci s canadensis L. 
Cerci s occidentalis Torr. 
Cerci s siliquastr um L. 
Chamaecr ista fasciculata (Michx. ) Greene 
Chamaesyce canescens (L.) Prokh. 
Chenopodium album L. 
Chionanthus EN Offi cial Jour nal of the European Union L 269/22                                                                                                                                         17.8.2020   Chitalpa tashk entensis T. S. Elias & Wisura 
Cistus 
Citrus 
Clematis cirrhosa L. 
Coelor achis cylindr ica (Michx.) Nash Coffea 
Conium maculatum L. 
Convo lvulus cneor um L. 
Copro sma repens A.Ri ch. 
Coron illa glauca (L.) Batt. 
Coron illa valentina L. 
Cyper us eragrostis Lam. 
Cytisus 
Digitar ia 
Dimor photeca 
Diosp yros kaki L.f. 
Diplocy clos palmatus (L.) C.Jeffre y 
Dodonaea viscosa (L.) Jacq. 
Elaeagnus angustif olia L. 
Encelia farinosa A. Gray ex Torr. 
Eremo phila maculata (Ker Gawler) F. von Müller . 
Erigeron 
Erodium moschatum (L.) L’Hér it. 
Erysimum 
Euphorbia chamaesyce L. 
Euphorbia terracina L. 
Euryops chrysanthemoides (DC.) B.Nord 
Euryops pectinatus (L.) Cass. 
Fagus crenata Blume 
Fallopia japonica (Houtt.) Ronse Decr . 
Fatsi a japonica (Thunb.) Decne. & Planch. 
Ficus carica L. 
Frangula alnus Mill. 
Fraxinus EN Offi cial Jour nal of the European Union 17.8.2020                                                                                                                                         L 269/23   Genista 
Ginkgo biloba L. 
Gleditsia triacanthos L. 
Grevil lea juniper ina Br. 
Hebe 
Helianthus 
Helichr ysum 
Heliotro pium europaeum L. 
Hemero callis 
Hevea brasiliensis (Willd. ex A.Juss.) Müll.Arg. 
Hibiscus 
Humulus scandens (Lour .) Mer r. 
Ilex aquifolium L. 
Ilex vomitor ia Sol. ex Aiton 
Iva annua L. 
Jacar anda mimosifolia D. Don 
Juglans 
Juniper us ashei J. Buchholz 
Koelre uteria bipinnata Franch. 
Lagerstroemia 
Laur us nobilis L. 
Lava ndula 
Ligustr um lucidum L. 
Liquidambar styraciflua L. 
Lonicer a japonica Thunb. 
Lupinus 
Magnolia grandif lora L. 
Mallotus paniculatus (Lam.) Müll.Arg. 
Malva parviflora L. 
Medicago arbor ea L. 
Medicago sativa L. 
Metros ideros 
Mimosa EN Offi cial Jour nal of the European Union L 269/24                                                                                                                                         17.8.2020   Modiola caroliniana (L.) G. Don 
Mor us 
Myo porum insulare R. Br. 
Myr tus communis L. 
Nandina domestica Mur ray 
Neptunia lutea (Leaven w.) Benth. 
Nerium oleander L. 
Olea 
Osteosper mum ecklonis DC. 
Osteosper mum fruticosum (L.) Norl. 
Parthenocissus quinquefolia (L.) Planch. 
Paspalum dilatatum Poir. 
Pelargonium 
Persea amer icana Mill. 
Phagnalon saxatile (L.) Cass. 
Phillyrea angustifolia L. 
Phillyrea latifolia L. 
Phlomis fruticosa L. 
Phoenix reclinata Jacq. 
Phoenix roebelenii O’ Brien 
Pinus taeda L. 
Pistacia vera L. 
Plantago lanceolata L. 
Platanus 
Pluchea odor ata (L.) Cass. 
Polygala myrtifolia L. 
Polygala x grandif lora Nana 
Prunus 
Ptero spar tum tridentatum (L.) Willk . 
Pyrus 
Quercus 
Ratibida columnife ra (Nutt.) Wooton & Standl. 
Rhamnus alater nus L. EN Offi cial Jour nal of the European Union 17.8.2020                                                                                                                                         L 269/25   Rhus 
Robinia pseudoacacia L. 
Rosa 
Rosmar inus officinalis L. 
Rubus 
Salvia mellifer a Greene 
Sambucus 
Santolina chamaecypar issus L. 
Sapindus saponar ia L. 
Sassafr as 
Setar ia magna Griseb. 
Solidago fistulosa Mill. 
Solidago virgaurea L. 
Sorghum halepense (L.) Pers. 
Spar tium 
Stewartia pseudocamellia 
Strelitzia reginae Aiton 
Streptocar pus 
Symphyo trichum divaricatum (Nutt.) G.L.Nesom 
Teucrium capitatum L. 
Trifolium repens L. 
UlexUlmus 
Vaccinium 
Vinca 
Vitis 
Westringia fruticosa (Willd.) Druce 
Westringia glabra R.Br . 
Xanthium strumar ium L.   EN Offi cial Jour nal of the European Union L 269/26                                                                                                                                         17.8.2020   ANNEX II 
List of plants kno wn to be susceptible to specif ic subspecies of the specif ied pest (‘specif ied plants’) 
Specif ied plants susceptible to Xylella fastidiosa subspecies fastidiosa 
Acer 
Ambro sia artemisiifolia L. 
Calicotome spinosa (L.) Link 
Cerci s occidentalis Torr. 
Cistus monspeliensis L. 
Citrus sinensis (L.) Osbeck 
Coffea arabica L. 
Erysimum 
Genista lucida L. 
Juglans regia L. 
Lupinus 
Magnolia grandif lora L. 
Malva parviflora L. 
Medicago sativa L. 
Metros ideros 
Mor us 
Nerium oleander L. 
Pluchea odor ata (L.) Cass. 
Polygala myrtifolia L. 
Prunus 
Rhamnus alater nus L. 
Rosmar inus officinalis L. 
Rubus rigidus Sm. 
Rubus ursinus Cham. & Schldl. 
Sambucus 
Spar tium junceum L. 
Streptocar pus 
Teucrium capitatum L. 
Ulmus amer icana L. 
Vinca major L. 
Vitis 
Specif ied plants susceptible to Xylella fastidiosa subspecies multiplex 
Acaci a 
Acer griseum (Franc h.) Pax 
Acer pseudoplatanus L. 
Acer rubrum L. 
Alnus rhombif olia Nutt. EN Offi cial Jour nal of the European Union 17.8.2020                                                                                                                                         L 269/27   Ambro sia psilostachy a DC. 
Ambro sia trifida L. 
Ampelopsis cordata Michx. 
Anthyllis hermanniae L. 
Artemisia 
Aspar agus acutif olius L. 
Bacchar is halimifolia L. 
Calicotome spinosa (L.) Link 
Calicotome villosa (Poir.) Link 
Callistemon citrinus (Cur tis) Skee ls 
Calluna vulgaris (L.) Hull 
Carya 
Celtis occidentalis L. 
Cerci s canadensis L. 
Cerci s occidentalis Torr. 
Cerci s siliquastr um L. 
Chionanthus 
Cistus 
Clematis cirrhosa L. 
Convo lvulus cneor um L. 
Copro sma repens A. Rich . 
Coron illa glauca (L.) Batt. 
Coron illa valentina L. 
Cytisus 
Dimor photeca 
Dodonaea viscosa (L.) Jacq. 
Elaeagnus angustif olia L. 
Encelia farinosa Gray ex Torr. 
Erigeron karvinskianus DC. 
Euryops chrysanthemoides (DC.) B.Nord. 
Euryops pectinatus (L.) Cass. 
Fallopia japonica (Houtt.) Ronse Decr . 
Ficus carica L. 
Frangula alnus Mill. 
Fraxinus 
Genista 
Ginkgo biloba L. 
Gleditsia triacanthos L. 
Grevil lea juniper ina Br. 
Hebe 
Helianthus 
Helichr ysum EN Offi cial Jour nal of the European Union L 269/28                                                                                                                                         17.8.2020   Ilex aquifolium L. 
Iva annua L. 
Koelre uteria bipinnata Franch. 
Lagerstroemia 
Laur us nobilis L. 
Lava ndula 
Liquidambar styraciflua L. 
Lupinus villosus Willd. 
Magnolia grandif lora L. 
Medicago arbor ea L. 
Medicago sativa L. 
Metros ideros excelsa Rich. 
Mor us 
Myr tus communis L. 
Olea 
Osteosper mum ecklonis (DC.) Norl. 
Pelargonium 
Phagnalon saxatile (L.) Cass. 
Phillyrea angustifolia L. 
Phlomis fruticosa L. 
Pistacia vera L. 
Plantago lanceolata L. 
Platanus 
Polygala myrtifolia L. 
Polygala x grandif lora Nana 
Prunus 
Ptero spar tum tridentatum (L.) Willk . 
Quercus 
Ratibida columnife ra (Nutt.) Wooton & Standl. 
Rhamnus alater nus L. 
Robinia pseudoacacia L. 
Rosa 
Rosmar inus officinalis L. 
Rubus 
Salvia mellifer a Greene 
Sambucus 
Santolina chamaecypar issus L. 
Sapindus saponar ia L. 
Solidago virgaurea L. 
Spar tium 
Strelitzia reginae Aiton 
Ulex EN Offi cial Jour nal of the European Union 17.8.2020                                                                                                                                         L 269/29   Ulmus amer icana L. 
Ulmus crassifo lia Nutt. 
Vaccinium 
Vinca 
Westringia fruticosa Guer in. 
Xanthium strumar ium L. 
Specif ied plants susceptible to Xylella fastidiosa subspecies pauca 
Acaci a 
Amar anthus retroflexus L. 
Aspar agus acutif olius L. 
Cathar anthus roseus (L.) G. Don 
Chamaesyce canescens (L.) Prokh. 
Chenopodium album L. 
Cistus albidus L. 
Cistus creticus L. 
Citrus 
Coffea 
Dimor photeca fruticosa (L.) DC. 
Dodonaea viscosa (L.) Jacq. 
Eremo phila maculata (Ker Gawler) F. von Müller . 
Erigeron 
Euphorbia chamaesyce L. 
Euphorbia terracina L. 
Grevil lea juniper ina Br. 
Hebe 
Heliotro pium europaeum L. 
Hibiscus 
Laur us nobilis L. 
Lava ndula angustif olia Mill. 
Lava ndula dentata L. 
Lava ndula stoechas L. 
Myo porum insulare Br. 
Myr tus communis L. 
Nerium oleander L. 
Olea europaea L. 
Osteosper mum fruticosum (L.) Norl. 
Pelargonium x fragrans Willd. (syn. Pelargonium fragrans E. Mey .) 
Phillyrea latifolia L. 
Polygala myrtifolia L. 
Prunus 
Rhamnus alater nus L. EN Offi cial Jour nal of the European Union L 269/30                                                                                                                                         17.8.2020   Rosmar inus officinalis L. 
Spar tium junceum L. 
Vinca minor L. 
Westringia fruticosa (Willd.) Druce 
Westringia glabra Br.   EN Offi cial Jour nal of the European Union 17.8.2020                                                                                                                                         L 269/31   ANNEX III 
Infected zones refer red to in Article 4(2) where cont ainment measures set out in Articles 13 to 17 
are applied 
PART A 
Infected zone in Italy 
The infect ed zone of Italy includes the follo wing areas: 
1. The province of Lecce 
2. The province of Brindisi 
3. Municipalities located in the province of Tarant o: 
Avetrana 
Carosino 
Crispiano 
Faggi ano 
Fragag nano 
Grottag lie 
Leporano 
Lizzano 
Mandur ia 
Mar tina Franca 
Mar uggio 
Monte iasi 
Monte mesola 
Monte parano 
Pulsano 
Roccaf orzata 
San Giorgio Ionico 
San Marzano di San Giuseppe 
Sava 
Statt e 
Taranto 
Torricella 
4. Municipality locat ed in the province of Bari: 
Locorot ondo 
PART B 
Infected zone in France 
The infect ed zone in France includes the followi ng area: 
The region of Corsica EN Offi cial Jour nal of the European Union L 269/32                                                                                                                                         17.8.2020   PART C 
Infec ted zone in Spain 
The infect ed zone in Spain includes the followi ng area: 
The Autonomous Community of Balear ic Islands   EN Offi cial Jour nal of the European Union 17.8.2020                                                                                                                                         L 269/33   ANNEX IV 
Tests for the identif ication of Xylella fastidiosa and its subspecies 
A. Tests for the screening and identif ication of the presence of Xylella fastidiosa 
1. Real time PCR based on Harper et al., 2010 (and erratum 2013); 
2. Loop-mediat ed isother mal amplification (LAMP) based on primers developed by Harper et al. 2010 (and erratum 
2013); 
3. Real time PCR based on Ouyang et al., 2013 
4. Conv entional PCR based on Minsavag e et al. 1994 
B. Molecular tests for the identif ication of the subspecies of Xylella fastidiosa 
1. Multi Locus Sequence Typing (MLST) based on Yuan et al., 2010 determining all subspecies; 
2. PCR based on Hernandez-Mar tinez et al., 2006 determining the subspecies fastidiosa, multiplex and sandyi; 
3. PCR based on Pooler & Hartung 1995 determining the subspecies pauca.   EN Offi cial Jour nal of the European Union L 269/34                                                                                                                                         17.8.2020   ANNEX V 
Templates for the repor ting of the results of the surveys carried out pursuant to Articles 10 and 15 in demarcated areas 
PART A 
Template for the repor t of results of statistically based annual surveys                                                                  1. Geographical location of the DA  
2. Initial size of DA (ha)  
3. Updated size of DA (ha)  
4. Approach (E/C)  
5. Zone (e.g. BZ/IZ)  
6. Survey sites  
7. Timing  A. Sur vey definition (input parameters for RiBESS+) B. Sampling effor t C. Sur vey results 
23. Comments  8. Target 
population 
9. Epidemiological units  
10. Detec tion 
method 
11. Sampling effec tiveness  
12. Method sensitivity  13. Risk factors (activities, 
locations and areas) 
14. N° epidemiological units inspect ed  
15. N° examinations  
16. N° samples  
17. N° tests  
18. N° other measures  
19. Results 20. 
Notifica-
tion 
number of 
the 
outbreaks 
notifi ed, 
as 
applic-
able, in 
accor -
dance 
with 
Imple-
menting 
Regula-
tion (EU) 
2019/17-
15 
21. Achieved Confidence level  
22. Design prevalence  Host species  
Area (ha or other more relevant unit))  
Inspection units  
Descr iption  
Units  
Visual examinations  
Testing  
Other methods  
Risk factor  
Risk levels  
N° of locations  
Relative risks  
Propor tion of the host population    
Positive  
Neg ative  
Undet ermined  
Number  
Date                                                                                                             EN Offi cial Jour nal of the European Union 17.8.2020                                                                                                                                         L 269/35   Instr uctions on how to fill in the template 
Explain the assum ptions for the survey design. Summar ise and justify: 
— The target population, epidemiological unit and inspection units; 
— The detection method and method sensitivity ; 
— The risk factor(s), indicating the risk levels and corresponding relative risks and propor tions of host plant population. 
For column 1: Indicate the name of the geographical area, outbreak number or any information that allows the identification of the Demarcate d Area (DA) concer ned and the date 
when it was established. 
For columns 2 and 3: Indicate the size of the DA before the start of the survey and any relevant update. 
For column 4: Indicate the approach: Eradication (E), Containment (C). Please, include as man y rows as necessar y, depending on the number of DA and the approaches these areas 
are subject to. 
For column 5: Indicate the zone of the DA where the survey was carried out, including as man y rows as necessar y: Infected/inf ested zone (IZ) or buffe r zone (BZ), using separate 
rows. When applicable, indicate the area of the IZ where the survey was carried out (e.g. last 5 km adjacent to the BZ, around nurser ies, etc.) in diffe rent rows . 
For column 6: Indicate the survey sites, using more than one row if needed. Please, alwa ys repor t the surveys carried out in nurser ies in a different row . When using the option 
‘other ’, please specify what it is: 
1. Open air (production area): 1.1 field (arable, pasture); 1.2. orchar d/vineyard; 1.3. nurser y; 1.4. forest ; 
2. Open air (others): 2.1. private gardens; 2.2. public sites; 2.3. conser vation area; 2.4. wild plants in areas other than conser vation areas; 2.5. other , with 
specifi cation of the particular case (e.g. garden centre, etc.); 
3. Physically closed conditions: 3.1. greenhouse; 3.2. private site, other than greenhouse; 3.3. public site, other than greenhouse; 3.4. other , with specifi cation of the 
particular case (e.g. garden centre). 
For column 7: Indicate the months of the year when the surveys were carried out. 
For column 8: Indicate the chosen target population providing according ly the list of host species and area covered. The target population is defined as the ensemble of inspection 
units. Its size is typically defined for agricultural areas as hectares, but it could be lots, fields, greenhouses, etc. Please justify the choice made in the underlying 
assum ptions in column 23 (‘Comments ’). Indicate the inspection units surveyed. ‘Inspection unit ’ means plants, plant parts, commodities, mater ials, pest vectors that 
had been scrutinised for identifying and detecting the pests. 
For column 9: Indicate the epidemiological units surveyed, indicating its descr iption and unit of measurement. ‘Epidemiological unit’ means a homogeneous area where the 
interactions between the pest, the host plants and the abiotic and biotic factors and conditions would result into the same epidemiology , should the pest be present. 
The epidemiological units are a subdivision of the target population which are homogenous in term s of epidemiology and which include at least one host plant. In 
some cases the whole host population in a region/area/countr y may be defined as epidemiological unit. They could be NUTS regions, urban areas, forests, rose 
gardens or farms, or hectares. The choice shall be justif ied in the underlying assum ptions. 
For column 10 Indicate the methods used during the survey including the number of activities in each case. Indicate with N/A when the information of certain column is not 
available. EN Offi cial Jour nal of the European Union L 269/36                                                                                                                                         17.8.2020   For column 11: Indicate an estimation of the sampling effectiveness. Sam pling effectiveness means the probability of selecting infect ed plant parts from an infected plant. For vect ors, 
it is the effectiveness of the method to captur e a positive vect or when it is present in the survey area. For soil, it is the effectiveness of selecting a soil sam ple 
containing the pest when the pest is present in the survey area. 
For column 12: ‘Method sensitivity’ means the probability of a method to correctly detec t pest presence. The method sensitivity is defined as the probability that a truly positive host 
tests positive. It is the multiplication of the sampling effectiveness (i.e. probability of selecting infec ted plant parts from an infect ed plant) by the diagnostic sensitivity 
(characteri sed by the visual inspection and/or laboratory test used in the identification process). 
For column 13: Prov ide the risk factors in differ ent rows, using as man y rows as necessar y. For each risk factor indicate the risk level and corresponding relative risk and propor tion 
of host population. 
For column B: Indicate the details of the survey. Indicate with N/A when the information of certain column is not applicable. The information to be provid ed in these columns is 
relat ed to the information included in the column 10 ‘Det ection methods’. 
For column 19: Indicate the number of samples found positive, negative or undete rmined. ‘Undet ermined’ are those analysed samples for whic h no result was obtained due to 
diffe rent factors (e.g. below detection level, unprocessed sam ple-not identif ied, old, etc.). 
For column 20: Indicate the outbreak notifi cations of the year when the survey took place. The outbreak notificati on number does not need to be included when the compet ent 
author ity has decided that the finding is one of the cases refer red to in Articles 14(2), 15(2) or 16 of Regulation (EU) 2016/2031. In this case, please indicate the 
reason for not providing this information in column 21 (‘Comments ’). 
For column 21: Indicate the sensitivity of the survey, as defined in ISPM 31. This value of the achieved confidence level of pest freedom is calculat ed based on the inspections (and/or 
sam ples) performe d given the method sensitivity and the design prevalence. 
For column 22: Indicate the design prevalence based on a pre-sur vey estimat e of the likely actual prevalence of the pest in the field. The design prevalence is set as a goal of the 
survey and corresponds to the compromise the risk managers are making between the risk of having the pest and the resources available for the survey. EN Offi cial Jour nal of the European Union 17.8.2020                                                                                                                                         L 269/37   PART B 
Template for the repor t of results of surveys carried out for insect vecto rs of Xylella fastidiosa                                                                  1. Descr iption of  
the DA  
2. Approach  
3. Zone  
4 Vector species  
5. Visual  
examinations  
6. Type of traps (or other vecto r  
capture method (e.g.,sw eep net, etc.)  
7. No of traps (or other capture  
method)  
8. Frequency of traps checking  
(or other method, if applicable)   
9. Timing of traps checking  
(or other method, if applicable)  
10. No of vecto r samples collected  
11. No of vecto rs trapped  
12. No of vecto rs analysed  
13. No of vecto r samples analysed  
14. No of positiv e vector samples  
15. No of negativ e vector samples  
16. No of undeter mined vecto r samples  
17. Comments  Name   
Date of  
establishment  
Timing  
Number                                          
Instr uctions on how to fill in the template 
For column1: Indicate the name of the area, outbreak number or any information that allows identifying the Demarcated Area (DA) and the date it was established. 
For column 2: Indicate : Eradication (E), Containment (C). Please include as many rows as necessar y, depending on the number of DA and the approaches these areas are subject to. 
For column 3: Indicate the zone of the DA where the survey was carried out: Infect ed/inf ested zone (IZ) or buffe r zone (BZ), using separate rows. When applicable, indicate the area of the 
IZ where the survey was carried out (e.g. last 5 km, around nurser ies, etc.). 
For column 4: Indicate the list of vect or species of the pest indicate d in the first column, using differ ent rows per vect or. 
For column 5: Indicate only if applicable. 
For column 6: Indicate the type of vect or capture method. When more than one method is used for the same vect or, provide the data in separate rows. 
For column 7: Indicate the number of traps or other capture method, using a separate row per method. 
For column 8: Indicate when the traps or captur e method were check ed (e.g. once per week, once per month, four times per year, etc.). 
For column 9: Indicate the months of the year when the traps are check ed. 
For column 10: Indicate the number of samples collected (a sample may contain several vect ors). 
For column 11: Indicate the total number of vect or trapped. Please include only the number of the vect or of interest, no bycatch. EN Offi cial Jour nal of the European Union L 269/38                                                                                                                                         17.8.2020   For column 13: Indicate the number of vect or samp les analysed for the pest, applicable when a sample is composed by more than one vect or. 
For column 16: The number of undeterm ined sam ples, namely samples analysed but where due to diffe rent factors no result was obtained (e.g. below detection level, etc.).’   EN Offi cial Jour nal of the European Union 17.8.2020                                                                                                                                         L 269/39