COMMISSION IMPLEMENTING REGUL ATION (EU) 2022/1421 
of 22 August 2022
concer ning the author isation of expressed orange essential oil, distilled orange essential oil and 
folded orange oils from Citr us sinensis (L.) Osbeck as feed additiv es for all animal species 
(Text with EEA relevance) 
THE EUR OPEAN COMMISSION,
Havi ng regar d to the Treaty on the Functioning of the European Union,
Havi ng rega rd to Regulation (EC) No 1831/2003 of the European Parliament and of the Council of 22 Sept ember 2003 on 
additives for use in animal nutr ition (1), and in particular Article 9(2) thereof,
Whereas:
(1) Regulation (EC) No 1831/2003 provid es for the author isation of additives for use in animal nutr ition and for the 
grounds and procedures for granting such an author isation. Article 10(2) of that Regulation provides for the 
re-evaluation of additives author ised pursuant to Council Directive 70/524/EEC (2).
(2) Expressed orange essential oil, distilled orange essential oil and folded orange oil were author ised without a time 
limit in accordance with Directive 70/524/EEC as feed additives for all animal species. These additives were 
subsequently entered in the Register of feed additives as existing products, in accordance with Article 10(1)(b) of 
Regulation (EC) No 1831/2003.
(3) In accordance with Article 10(2) of Regulation (EC) No 1831/2003 in conjunction with Article 7 thereof, an 
application was submitt ed for the re-evaluation of expressed orange essential oil, distilled orang e essential oil and 
folded orang e oils from Citrus sinensis (L.) Osbec k for all animal species.
(4) The applicant requeste d the additives to be classified in the additive cate gory ‘sensor y additives ’ and in the functional 
group ‘flavouring compo unds ’. That application was accompan ied by the particulars and documents required under 
Article 7(3) of Regulation (EC) No 1831/2003.
(5) The applicant request ed those additives to be author ised also for use in water for drinking. Howe ver, Regulation (EC) 
No 1831/2003 does not allow the author isation of flavouring compo unds for use in water for drinking. Theref ore, 
the use of these additives in wate r for drinking should not be allowed.
(6) The European Food Safety Author ity (‘the Auth ority’) concluded in its opinion of 29 September 2021 (3)that, under 
the proposed conditions of use, expressed orange essential oil, distilled orang e essential oil and folded orange oils 
from Citrus sinensis (L.) Osbeck do not have adverse effects on animal health, consumer health or the environment. 
However , no conclusions could be drawn for dogs, cats, ornamental fish and ornamental birds not normally 
exposed to citrus by-products. The Author ity also concluded that expressed orange essential oil, distilled orange 
essential oil and folded orang e oils from Citrus sinensis (L.) Osbeck should be considered as irritants to skin and eyes, 
and as skin and respirator y sensitisers. Theref ore, the Commission considers that appropr iate protective measures 
should be taken to prevent adverse effects on human health, in particular as regar ds the users of the additives.
(1)OJ L 268, 18.10.2003, p. 29.
(2)Council Directive 70/524/EEC of 23 November 1970 concer ning additives in feedingstuffs (OJ L 270, 14.12.1970, p. 1).
(3)EFSA Jour nal 2021;19(11):6891.EN Offi cial Jour nal of the European Union 23.8.2022 L 218/27  (7) The Author ity also concluded, that expressed orange essential oil, distilled orange essential oil and folded orange oils 
from Citrus sinensis (L.) Osbeck are recognised to flavour food and their function in feed would be essentially the 
same as that in food, theref ore, no further demonstration of efficacy is considered necessar y. It also verified the 
repor t on the methods of analysis of the feed additive in feed submitte d by the Reference Laboratory set up by 
Regulation (EC) No 1831/2003.
(8) The assessment of expressed orang e essential oil, distilled orange essential oil and folded orang e oils from Citrus 
sinensis (L.) Osbeck shows that the conditions for author isation, as provi ded for in Article 5 of Regulation (EC) 
No 1831/2003, are satisfied. According ly, the use of these substances should be author ised as specifi ed in the 
Annex to this Regulation.
(9) Certain conditions should be provid ed for to allow better control. In particular , a recommended content should be 
indicated on the label of the feed additives. Where such content is exceeded, certain information should be indicated 
on the label of premixtures.
(10) The fact that the additives are not author ised for use as flavourings in water for drinking does not preclude their use 
in compo und feed whic h is administ ered via water .
(11) Since safet y reasons do not require the immediate application of the modifications to the conditions of author isation 
of the substances concer ned, it is appropr iate to allow a transitional period for interest ed parties to prepare 
themselves to meet the new requirements resulting from the author isation.
(12) The measures provid ed for in this Regulation are in accordance with the opinion of the Standing Committ ee on 
Plants, Animals, Food and Feed,
HAS ADOPTED THIS REGUL ATION:
Article 1
Author isation
The substances specifi ed in the Annex, belonging to the additive cate gory ‘sensor y additives ’ and to the functional group 
‘flavouring compo unds ’, are author ised as feed additives in animal nutr ition, subject to the conditions laid down in that 
Annex.
Article 2
Transitional measures
1. The substances specifi ed in the Annex and premixtures containing these substances, which are produced and labelled 
before 12 March 2023 in accordance with the rules applicable before 12 September 2022 may continue to be placed on the 
mark et and used until the existing stoc ks are exhaust ed.
2. Com pound feed and feed mate rials containing these substances as specified in the Annex which are produced and 
labelled before 12 Sept ember 2023 in accordance with the rules applicable before 12 Sept ember 2022 may continue to be 
placed on the market and used until the existing stoc ks are exhaust ed if they are intended for food-producing animals.
Article 3
Entr y into force
This Regulation shall enter into force on the twentieth day followi ng that of its publication in the Official Journal of the 
European Union.EN Offi cial Jour nal of the European Union L 218/28 23.8.2022  This Regulation shall be binding in its entirety and directly applicable in all Member States.
Done at Brussels, 22 August 2022 .
For the Commission
The President
Ursula VON DER LEYENEN Offi cial Jour nal of the European Union 23.8.2022 L 218/29  ANNEX 
Identification 
number of 
the additiveAdditiveComposition, chemical formula, 
descr iption, analytical method.Species or categor y of 
animalMaximum 
ageMinimum 
contentMaximum 
conten t
Other provisionsEnd of period 
of 
author isationmg additive/kg of 
compl ete feed with a 
moisture conten t of 
12 %
Categor y: Sensor y additiv es. Functional group: Flavour ing compounds
2b143-eo Expressed 
orange 
essential oilAdditive composition
Expressed orange essential oil from 
fruit peels of Citrus sinensis (L.) 
Osbeck (1).
Liquid form
Char acter isation of the active substance
Essential oil derived by cold 
expression from fruit peel of Citrus 
sinensis (L.) Osbeck as defined by the 
Council of Europe (2).
In the volatile fraction:
d-Limonene: 93-97 %
Myrcene: 1,5-3,5 %
Sabinene: 0,1-1,0 %
α-Pinene: 0,4-0,8 %
Linalool: 0,1-0,7 %
Decanal: 0,1-0,7 %
Octanal: 0,1-0,6 %Chick ens for 
fattening and other 
minor poultr y 
species for fattening
Laying hens and 
other minor 
poultr y species for 
laying and breeding 
purposes
Turkeys for 
fattening- - 80 1.The additive shall be incor porated into the feed 
in the form of a premixture.
2.In the directions for use of the additive and 
premixtures, the storag e conditions and stabi ­
lity to heat treatment shall be indicated.
3.Mixture with other botanical additives is per­
mitted provided that the amounts of perillalde ­
hyde added to feedingstuffs by such mixtures is 
lower than the one resulting from the use of a 
sing le additive at the maximum or recom ­
mended level for the species or categor y of ani­
mal.
4.For users of the additive and premixtures, feed 
business operat ors shall establish operational 
procedures and organisational measures to ad­
dress poten tial risks by inhalation, dermal con­
tact or eyes contact. Where those risks cannot 
be eliminated or reduced to a minimum by such 
procedures and measures, the additive and pre­
mixtures shall be used with personal prot ective 
equipment, including skin, eye and breathing 
prote ction.12.9.2032
All Suidae for 
fattening- - 172
Piglets of all Suidae 
species- - 144EN Offi cial Jour nal of the European Union L 218/30 23.8.2022  Perillaldeh yde: < 0,05 %
CAS number: 8028-48-6
Einecs number: 232-433-8
FEMA number: 2825
CoE number: 143
Analytical method (3)
For the determination of d-limonene 
(phyt ochemical mark er) in the feed 
additive:
— gas chromat ography coupled with 
flame ionisation detection 
(GC-FID) (based on ISO 3140)Sows - - 200
Ruminants - - 130
Horses - - 230
Rabbits
Fish excep t 
ornamental fish- - 50
Other species - - 50
(1)Synony ms: Citrus sinensis (L.) Pers. , Citrus aurantium (L.) Dulcis.
(2)Natural sources of flavourings – Repor t No 2 (2007).
(3)Details of the analytical methods are available at the following address of the Reference Laborato ry: https://joint-research-c entre.ec.europa.eu/eurl-fa-eurl-f eed-additives/eurl-fa-author isation/eurl-f a-evaluation- 
repor ts_enEN Offi cial Jour nal of the European Union 23.8.2022 L 218/31  Identification 
number of 
the additiveName of the 
holder of 
author isation
AdditiveComposition, chemical formula, 
descr iption, analytical method.Species or categor y of 
animalMaximum 
ageMinimum 
contentMaximum 
conten t
Other provisionsEnd of period 
of 
author isationmg additive/kg of 
compl ete feed with a 
moisture conten t of 
12 %
Categor y: Sensor y additiv es. Functional group: Flavour ing compounds
2b143-di Distilled 
orange 
essential oilAdditive composition
Distillat e (volati le fraction) of orang e 
essential oil, whic h is obtained from 
fruit peel of Citrus sinensis (L.) Osbec k.
Liquid form
Char acter isation of the active substance
Volatile fraction from the distillation 
of orang e essential oil expressed (cold 
pressed), whic h is obtained from the 
fruit peel of Citrus sinensis (L.) Osbeck 
as defined by the Council of Europe (1).
d-Limonene: 93-97,5 %
Myrcene: 1,5-3,5 %
Sabinene: 0,2-1,0 %
α-Pinene: 0,3-0,8 %
Linalool: 0,05-0,5 %
Octanal: 0,05-0,4 %
Perillaldeh yde: < 0,005 %Chick ens for 
fattening and other 
minor poultr y 
species for fattening
Laying hens and 
other minor 
poultr y species for 
laying and breeding 
purposes
Turkeys for 
fattening- - 80 1.The additive shall be incor porated into the feed 
in the form of a premixture.
2.In the directions for use of the additive and 
premixtures, the storag e conditions and stabi ­
lity to heat treatment shall be indicated.
3.Mixture with other botanical additives is per­
mitted provided that the amounts of perillalde ­
hyde added to feedingstuffs by such mixtures is 
lower than the one resulting from the use of a 
sing le additive at the maximum or recom ­
mended level for the species or categor y of ani­
mals.
4.For users of the additive and premixtures, feed 
business operat ors shall establish operational 
procedures and organisational measures to ad­
dress poten tial risks by inhalation, dermal con­
tact or eyes contact. Where those risks cannot 
be eliminated or reduced to a minimum by such 
procedures and measures, the additive and pre­
mixtures shall be used with personal prot ective 
equipment, including skin, eye and breathing 
prote ction.12.9.2032
Suidae - - 200
Ruminants - - 130
Horses - - 225EN Offi cial Jour nal of the European Union L 218/32 23.8.2022  CAS number: 8028-48-6
CoE number: 143
Analytical method (2)
For the determination of d-limonene 
(phyt ochemical mark er) in the feed 
additive:
— gas chromat ography coupled with 
flame ionisation detection 
(GC-FID) (based on ISO 3140)Rabbits
Fish excep t 
ornamental fish- - 80
Other species - - 80
(1)Natural sources of flavourings – Repor t No 2 (2007).
(2)Details of the analytical methods are available at the following address of the Reference Laborato ry: https://joint-research-c entre.ec.europa.eu/eurl-fa-eurl-f eed-additives/eurl-fa-author isation/eurl-f a-evaluation- 
repor ts_enEN Offi cial Jour nal of the European Union 23.8.2022 L 218/33  Identification 
number of 
the additiveName of the 
holder of 
author isation
AdditiveComposition, chemical formula, 
descr iption, analytical method.Species or categor y of 
animalMaximum 
ageMinimum 
contentMaximum 
conten t
Other provisionsEnd of period 
of 
author isationmg additive/kg of 
compl ete feed with a 
moisture conten t of 
12 %
Categor y: Sensor y additiv es. Functional group: Flavour ing compounds
2b143-f Folded orange 
oilAdditive composition
Folded oil obtained from the fruit peel 
of Citrus sinensis (L.) Osbeck.
Liquid form
Char acter isation of the active substance
Folded oil produced by the fractional 
distillation of an expressed orange 
essential oil obtained from the fruit 
peel of Citrus sinensis (L.) Osbec k as 
defined by the Council of Europe (1).
Non-v olatile fraction: 10,5 %
In the volatile fraction:
d-Limonene: 89-96 %
Decanal: 0,5-2 %
Linalool: 0,7-1,7 %
Myrcene: 0,1-1,0 %
Geranial: 0,1-1,0 %Chick ens for 
fattening and other 
minor poultr y 
species for fattening- - 15,5 1.The additive shall be incor porated into the feed 
in the form of a premixture.
2.In the directions for use of the additive and 
premixtures, the storag e conditions and stabi ­
lity to heat treatment shall be indicated.
3.Mixture with other botanical additives is per­
mitted provided that the amounts of perillalde ­
hyde added to feedingstuffs by such mixtures is 
lower than the one resulting from the use of a 
sing le additive at the maximum or recom ­
mended level for the species or categor y of ani­
mals.
4.For users of the additive and premixtures, feed 
business operat ors shall establish operational 
procedures and organisational measures to ad­
dress poten tial risks by inhalation, dermal con­
tact or eyes contact. Where those risks cannot 
be eliminated or reduced to a minimum by such 
procedures and measures, the additive and pre­
mixtures shall be used with personal prot ective 
equipment, including skin, eye and breathing 
prote ction.12.9.2032
Laying hens and 
other minor 
poultr y species for 
laying and breeding 
purposes- - 23,5
Turkeys for 
fattening- - 21
All Suidae for 
fattening- - 34
Piglets of all Suidae 
species- - 28,5
Sows - - 41,5
Calves (milk 
replacer)- - 66,5EN Offi cial Jour nal of the European Union L 218/34 23.8.2022  Perillaldeh yde: < 0,3 %
CAS number: 8028-48-6
FEMA number: 2822
Analytical method (2)
For the determination of d-limonene 
(phyt ochemical mark er) in the feed 
additive:
— gas chromat ography coupled with 
flame ionisation detection 
(GC-FID) (based on ISO 3140)Ruminants for 
fattening- - 62,5
Dair y ruminants - - 40,5
Horses - - 62,5
Rabbits - - 25
Fish excep t 
ornamental fish- - 70
Other species - - 15,5
(1)Natural sources of flavourings – Repor t No 2 (2007).
(2)Details of the analytical methods are available at the following address of the Reference Laborato ry: https://joint-research-c entre.ec.europa.eu/eurl-fa-eurl-f eed-additives/eurl-fa-author isation/eurl-f a-evaluation- 
repor ts_enEN Offi cial Jour nal of the European Union 23.8.2022 L 218/35  Identification 
number of 
the additiveName of the 
holder of 
author isation
AdditiveComposition, chemical formula, 
descr iption, analytical method.Species or categor y of 
animalMaximum 
ageMinimum 
contentMaximum 
conten t
Other provisionsEnd of period 
of 
author isationmg additive/kg of 
compl ete feed with a 
moisture conten t of 
12 %
Categor y: Sensor y additiv es. Functional group: Flavour ing compounds
2b143-f-i Folded orange 
oilAdditive composition
Folded oil obtained from the fruit peel 
of Citrus sinensis (L.) Osbeck.
Liquid form
Char acter isation of the active substance
Folded oil produced by the fractional 
distillation of an expressed orange 
essential oil obtained from the fruit 
peel of Citrus sinensis (L.) Osbec k as 
defined by the Council of Europe (1).
Non-v olatile fraction: 20,9 %
In the volatile fraction:
d-Limonene: 79-89 %
Decanal: 3,0-5,0 %
Linalool: 2,0-5,0 %
Myrcene: 0,1-1,0 %
Geranial: 0,5-1,8 %
Perillaldeh yde: < 0,6 %Chick ens for 
fattening and other 
minor poultr y 
species for fattening- - 5,5 1.The additive shall be incor porated into the feed 
in the form of a premixture.
2.In the directions for use of the additive and 
premixtures, the storag e conditions and stabi ­
lity to heat treatment shall be indicated.
3.Mixture with other botanical additives is per­
mitted provided that the amounts of perillalde ­
hyde added to feedingstuffs by such mixtures is 
lower than the one resulting from the use of a 
sing le additive at the maximum or recom ­
mended level for the species or categor y of ani­
mals.
4.For users of the additive and premixtures, feed 
business operat ors shall establish operational 
procedures and organisational measures to ad­
dress poten tial risks by inhalation, dermal con­
tact or eyes contact. Where those risks cannot 
be eliminated or reduced to a minimum by such 
procedures and measures, the additive and pre­
mixtures shall be used with personal prot ective 
equipment, including skin, eye and breathing 
prote ction.12.9.2032
Laying hens and 
other minor 
poultr y species for 
laying and breeding 
purposes- - 8
Turkeys for 
fattening- - 7
All Suidae for 
fattening- - 11,5
Piglets of all Suidae 
species- - 9,5
Sows - - 14EN Offi cial Jour nal of the European Union L 218/36 23.8.2022  CAS number: 8028-48-6
FEMA number: 2822
Analytical method (2)
For the determination of d-limonene 
(phyt ochemical mark er) in the feed 
additive:
— gas chromat ography coupled with 
flame ionisation detection 
(GC-FID) (based on ISO 3140)Calves (milk 
replacer)- - 23
Ruminants for 
fattening- - 21,5
Dair y ruminants - - 14
Horses - - 21,5
Rabbits - - 8,5
Fish excep t 
ornamental fish- - 24,5
Other species - - 5,5
(1)Natural sources of flavourings – Repor t No 2 (2007).
(2)Details of the analytical methods are available at the following address of the Reference Laborato ry: https://joint-research-c entre.ec.europa.eu/eurl-fa-eurl-f eed-additives/eurl-fa-author isation/eurl-f a-evaluation- 
repor ts_enEN Offi cial Jour nal of the European Union 23.8.2022 L 218/37  Identification 
number of 
the additiveAdditiveComposition, chemical formula, 
descr iption, analytical method.Species or categor y of 
animalMaximum 
ageMinimum 
contentMaximum 
conten t
Other provisionsEnd of period 
of 
author isationmg additive/kg of 
compl ete feed with a 
moisture conten t of 
12 %
Categor y: Sensor y additiv es. Functional group: Flavour ing compounds
2b143-f-ii Folded orange 
oilAdditive composition
Folded oil obtained from the fruit peel 
of Citrus sinensis (L.) Osbeck.
Liquid form
Char acter isation of the active substance
Folded oil produced by the fractional 
distillation of an expressed orange 
essential oil which is obtained from 
the fruit peel of Citrus sinensis (L.) 
Osbeck as defined by the Council of 
Europe (1).
Non-v olatile fraction: 18 %
In the volatile fraction:
d-Limonene: 85-95 %
Linalool: 0,5-4 %
CAS number: 8028-48-6
FEMA number: 2822All animal species - - - 1.The additive shall be incor porated into the feed 
in the form of a premixture.
2.In the directions for use of the additive and 
premixtures, the storag e conditions and stabi ­
lity to heat treatment shall be indicated.
3.On the label of the additive the follo wing shall 
be indicated:
‘Recommended maximum conte nt of the active 
substance per kg of complet e feedingstuff with a 
moisture conte nt of 12 %:
— Chick ens for fattening and other minor 
poultr y species for fattening, laying hens 
and other minor poultr y species for laying 
and breeding purposes, turke ys for fatten ­
ing, Suidae: 50 mg;
— Calves (milk replacer): 70 mg;
— Ruminants excep t sheep and goats: 60 mg;
— Sheep and goats: 70 mg;
— Fish, ornamental fish: 2 mg:
— Other terrestr ial animals: 50 mg’.
4.The functional group, the identif ication num ­
ber, the name and the added amount of the 
active substance shall be indicated on the label 
of the premixture where the use level on the 
label of the premixture would result in exceed ­
ing the level referred to in point 3.12.9.2032EN Offi cial Jour nal of the European Union L 218/38 23.8.2022  Analytical method (2)
For the determination of d-limonene 
(phyt ochemical mark er) in the feed 
additive:
— gas chromat ography coupled with 
flame ionisation detection 
(GC-FID) (based on ISO 3140)5.For users of the additive and premixtures, feed 
business operat ors shall establish operational 
procedures and organisational measures to ad­
dress poten tial risks by inhalation, dermal con­
tact or eyes contact. Where those risks cannot 
be eliminated or reduced to a minimum by such 
procedures and measures, the additive and pre­
mixtures shall be used with personal prot ective 
equipment, including skin, eye and breathing 
prote ction.
(1)Natural sources of flavourings – Repor t No 2 (2007).
(2)Details of the analytical methods are available at the following address of the Reference Laborato ry: https://joint-research-c entre.ec.europa.eu/eurl-fa-eurl-f eed-additives/eurl-fa-author isation/eurl-f a-evaluation- 
repor ts_enEN Offi cial Jour nal of the European Union 23.8.2022 L 218/39