COMMISSION IMPLEMENTING REGUL ATION (EU) 2024/1056 
of 10 Apr il 2024
concer ning the author isation of ribof lavin 5’-phosphate monosodium salt (vitamin B2), produced by 
Bacillus subtilis KCCM 10445, as a feed additiv e for all animal species 
(Text with EEA relevance) 
THE EUR OPEAN COMMISSION,
Havi ng regar d to the Treaty on the Functioning of the European Union,
Havi ng rega rd to Regulation (EC) No 1831/2003 of the European Parliament and of the Council of 22 Sept ember 2003 on 
additives for use in animal nutr ition (1), and in particular Article 9(2) thereof,
Whereas:
(1) Regulation (EC) No 1831/2003 provid es for the author isation of additives for use in animal nutr ition and for the 
grounds and procedures for granting such an author isation.
(2) In accordance with Article 7 of Regulation (EC) No 1831/2003, an application was submitted for the author isation 
of ribof lavin 5’-phosphate monosodium salt (vitamin B2) produced by Bacillus subtilis KCCM 10445. That 
application was accompanied by the particulars and documents required under Article 7(3) of Regulation (EC) 
No 1831/2003.
(3) The application concer ns the author isation of ribof lavin 5’-phosphate monosodium salt (vitamin B2) produced by 
Bacillus subtilis KCCM 10445, as a feed additive for all animal species, requesting that additive to be classified in the 
catego ry ‘nutr itional additives ’ and in the functional group ‘vitamins, pro-vitamins and chemically well-defined 
substances having similar effect’. The applicant also requeste d the additive to be author ised for use in water for 
drinking.
(4) The European Food Safety Author ity (‘the Auth ority’) concluded in its opinion of 27 September 2022 (2)that, under 
the proposed conditions of use, ribof lavin 5’-phosphate monosodium salt (vitamin B2) produced by Bacillus subtilis 
KCCM 10445 is safe for all animal species, consumers and the envi ronment. It also concluded that is not a skin/e ye 
irritant, and it is not considered a respirato ry sensitiser and that ribof lavin is a known photosensitiser whic h may 
elicit skin and eye photoaller gic reactions. The Author ity further concluded that ribof lavin 5’-phosphate 
monosodium salt (vitamin B2) produced by Bacillus subtilis KCCM 10445 is effective in covering the animals’ 
requirements for vitamin B2, when administe red via feed and/or wate r for drinking. It did not consider that there is 
a need for specific requirements of post-market monitori ng. The Author ity also verified the repor t on the methods 
of analysis of the feed additive in feed submitted by the Reference Laboratory set up by Regulation (EC) 
No 1831/2003.
(5) In view of the abov e, the Commission considers that ribof lavin 5’-phosphate monosodium salt (vitamin B2) 
produced by Bacillus subtilis KCCM 10445 satisfi es the conditions for author isation provid ed for in Article 5 of 
Regulation (EC) No 1831/2003. According ly, the use of that substance should be author ised. In addition, the 
Commission considers that appropr iate protect ive measures should be take n to prevent adverse effects the health of 
the users of the additive.
(6) The measures provid ed for in this Regulation are in accordance with the opinion of the Standing Committ ee on 
Plants, Animals, Food and Feed,
Offi cial Jour nal 
of the European Union EN 
L series 
2024/1056 11.4.2024
ELI: http://data.europa.eu/eli/reg_impl/2024/1056/oj 1/4(1) OJ L 268, 18.10.2003, p. 29. ELI: http://data.europa.eu/eli/reg/2003/1831/oj
(2) EFSA Journal 2022;20(11):7608.HAS ADOPTED THIS REGUL ATION:
Article 1
Author isation
The substance specifi ed in the Annex, belonging to the additive categor y ‘nutr itional additives’ and to the functional group 
‘vitamins, pro-vitamins and chemically well-def ined substances having similar effect’, is author ised as an additive in animal 
nutr ition, subject to the conditions laid down in that Annex.
Article 2
Entr y into force
This Regulation shall enter into force on the twentieth day followi ng that of its publication in the Official Journal of the 
European Union.
This Regulation shall be binding in its entirety and directly applicable in all Member States.
Done at Brussels, 10 Apr il 2024 .
For the Commission
The President
Ursula VON DER LEYENEN OJ L, 11.4.2024 
2/4 ELI: http://data.europa.eu/eli/reg_impl/2024/1056/ojANNEX 
Identifica -
tion 
number of 
the feed 
additiveAdditiveComposition, chemical formula, descr iption, 
analytical methodSpecies or 
category 
of animalMaxi -
mum 
ageMinimum 
contentMaximum 
content
Other provisionsEnd of period of 
author isation mg of active substance/kg of 
complet e feed ingstuff with a 
moisture content of 12 %
Categor y: nutr itional additiv es. Functional group: vita mins, pro-vitamins and chemically well-def ined subst ances having similar effect
3a826i ‘Ribof lavin 5’- 
phosphate 
monosodium 
salt’ or ‘Vitamin 
B2’Additive composition
Ribof lavin 5’-phosphate ester monosodium 
salt
Solid form
Char acter isation of activ e substance
Ribof lavin 5’-phosphate ester monosodium 
salt
Chemical formula: C17H20N4O9PNa
CAS number: 130–40-5
Content: 73-79 % of ribof lavin on dry mater
Ribof lavin 5’-phosphate ester monosodium 
salt produced after phosphor ylation of ribo­
flavin 98 %, produced by Bacillus subtilis 
KCCM 10445
Analytical method (1)
For the determination of ribof lavin 5’-phos ­
phate ester monosodium salt in the feed 
additive: Spectrophotom etry at 444 nm Eur­
opean Phar macopoeia Monograph 0786.
For the determination of ribof lavin 5’-phos ­
phate ester monosodium salt (as total vitamin 
B2) in premixtures: High Performan ce Liquid 
Chromatograph y with Fluorescence detec­
tion (HPL C-FLD).All 
animal 
species1. The additive may be used 
via water for drinking.
2. The directions for use of the 
additive and premixture 
shall indicate the storag e 
conditions, the stability to 
heat treatment and the sta­
bility in water for drinking.
3. For users of the additive and 
premixtures, feed business 
operators shall establish 
operational procedures and 
orga nisational measures to 
address potential risks. 
Where those risks cannot 
be eliminated by such pro­
cedures and measures, the 
additive and premixtures 
shall be used with appro ­
priate personal prot ective 
equipment, including skin 
and eye protection.1 May 2034OJ L, 11.4.2024 EN 
ELI: http://data.europa.eu/eli/reg_impl/2024/1056/oj 3/4Identifica-
tion 
number of 
the feed 
additiveAdditiveComposition, chemical formula, descr iption, 
analytical methodSpecies or 
category 
of animalMaxi-
mum 
ageMinimum 
contentMaximum 
content
Other provisionsEnd of period of 
author isation mg of active substance/kg of 
complet e feed ingstuff with a 
moisture content of 12 %
For the determination of ribof lavin 5’-phos ­
phate ester monosodium salt (as total vitamin 
B2) in compo und feed and water: High Per­
formance Liquid Chromat ography with 
Fluorescence detection (HPL C-FLD) EN 
14152.
(1)Details of the analytical methods are available at the following address of the Reference Laboratory : https://ec.europa.eu/jrc/en/eurl/feed-additives/evaluation-repor tsEN OJ L, 11.4.2024 
4/4 ELI: http://data.europa.eu/eli/reg_impl/2024/1056/oj