EN 
ANNEX  
ANNEX I  
List of plants known to be susceptible to one or more  subspecies of the specified pest 
(‘host plants’)  
Acacia  
Acer  
Albizia julibrissin  Durazz.  
Alnus rhombifolia  Nutt.  
Amaranthus retroflexus L. 
Ambrosia   
Ampelopsis arborea  (L.) Koehne   
Ampelopsis brevipedunculata  (Maxim.) Trautv.  
Ampelopsis cordata  Michx.  
Anthyllis hermanniae  L. 
Artemisia  
Asparagus acutifolius  L. 
Baccharis  
Brassica  
Calicotome spinosa  (L.) Link  
Calicotome villosa  (Poiret) Link  
Callicarpa americana  L. 
Callistemon citrinus (Curtis) Skeels  
Calluna vulgaris   (L.) Hull  
Carya  
Catharanthus Celtis occidentalis  L. 
Cercis canadensis  L. 
Cercis occidentalis  Torr.  
Cercis siliquastrum  L. 
Chamaecrista fasciculata  (Michx.) Greene  
Chamaesyce canescens (L.) Prokh.  
Chenopodium album  L. 
Chionanthus   
Chitalpa tashkentensis  T. S. Elias & Wisura  
Cistus  
Citrus  
Clematis cirrhosa L. 
Coelorachis cylindrica  (Michx.) Nash 
Coffea  Conium maculatum  L. 
Convolvulus cneorum  L. 
Coprosma repens A.Rich.  
Coronilla glauca  (L.) Batt.  
Coronilla valentina  L. 
Cyperus eragrostis  Lam.  
Cytisus  
Digitaria  
Dimorphoteca  
Diospyros kaki L.f. 
Diplocyclos palmatus  (L.) C.Jeffrey   
Dodonaea viscosa  (L.) Jacq.  
Elaeagnus angustifolia  L. 
Encelia farinosa  A. Gray ex Torr.  
Eremophila maculata  (Ker Gawler) F. von Müller.  
Erigeron  
Erodium moschatum  (L.) L’H ér 
Erysimum  
Euphorbia chamaesyce L. 
Euphorbia terracina  L. 
Euryops chrysanthemoides  (DC.) B.Nord  
Euryops pectinatus (L.) Cass.  
Fagus crenata  Blume  
Fallopia japonica  (Houtt.) Ronse Decr.  
Fatsia japonica  (Thunb.) Decne. & Planch.  
Ficus carica  L. 
Frangula alnus   Mill. 
Fraxinus  
Genista  
Ginkgo biloba  L. 
Gleditsia triacanthos  L. 
Grevillea juniperina  Br. 
Hebe  
Helianthus  
Helichrysum  
Heliotropium europaeum  L. 
Hemerocallis  
Hevea brasiliensis  (Willd. ex A.Juss.) Müll.Arg.  
Hibiscus  
Humulus scandens  (Lour.) Merr.  
Ilex aquifolium L. Ilex vomitoria  Sol. ex Ai ton  
Iva annua  L. 
Jacaranda mimosifolia  D. Don  
Juglans  
Juniperus ashei  J. Buchholz  
Koelreuteria bipinnata  Franch.  
Lagerstroemia  
Laurus nobilis L. 
Lavandula   
Ligustrum lucidum  L. 
Liquidambar styraciflua  L. 
Lonicera japonica  Thunb.  
Lupinus  
Magnolia grandiflora  L. 
Mallotus paniculatus  (Lam.) Müll.Arg.  
Malva parviflora L. 
Medicago arborea L. 
Medicago sativa  L. 
Metrosideros  
Mimosa  
Modiola caroliniana  (L.) G. Don  
Morus  
Myoporum insulare  R. Br. 
Myrtus communis  L. 
Nandina domestica  Murray  
Neptunia lutea  (Leavenw.) Benth.   
Nerium oleander  L. 
Olea  
Osteospermum ecklonis  DC. 
Osteospermum fruticosum (L.) Norl.  
Parthenocissus quinquefolia  (L.) Planch.  
Paspalum dilatatum  Poir.  
Pelargonium  
Persea americana  Mill.  
Phagnalon saxatile  (L.) Cass.   
Phillyrea angustifolia L. 
Phillyrea latifolia  L. 
Phlomis fruticosa L. 
Phoenix  reclinata  Jacq.  
Phoenix  roebelenii  O' Brien   
Pinus  taeda  L. Pistacia vera L. 
Plantago lanceolata  L. 
Platanus  
Pluchea odorata  (L.) Cass . 
Polygala myrtifolia  L. 
Polygala x grandiflora nana   
Prunus  
Pterospartum tridentatum  (L.) Willk.  
Pyrus   
Quercus  
Ratibida columnifera  (Nutt.) Wooton &  Standl.  
Rhamnus alaternus  L. 
Rhus  
Robinia pseudoacacia L. 
Rosa  
Rosmarinus officinalis  L. 
Rubus  
Salvia mellifera  Greene  
Sambucus  
Santolina chamaecyparissus L. 
Sapindus saponaria  L. 
Sassafras  
Setaria magna  Griseb.  
Solidago fistulosa  Mill.  
Solidago virgaurea  L. 
Sorghum halepense  (L.) Pers.   
Spartium  
Stewartia pseudocamellia  
Strelitzia reginae Aiton  
Streptocarpus  
Symphyotrichum divaricatum  (Nutt.) G.L.Nesom  
Teucrium capitatum L. 
Trifolium repens  L. 
Ulex Ulmus  
Vaccinium  
Vinca  
Vitis 
Westringia fruticosa (Willd.) Druce  
Westringia glabra R.Br.  
Xanthium strumarium L. 
  ANNEX II  
List of plants known to be susceptible to specific subspecies of the specified pest 
(‘specified plants’)  
 
Specified plants susceptible to Xylella fastidiosa subspecies fastidiosa  
Acer  
Ambrosia artemisiifolia L. 
Calicotome spinosa  (L.) Link  
Cercis occidentalis  A. Gray  
Cistus monspeliensis  L. 
Citrus sinensis  (L.) Osbeck  
Coffea arabica  L. 
Erysimum   
Genista lucida  L. 
Juglans regia  L. 
Lupinus   
Magnolia grandiflora  L. 
Malva parviflora  L. 
Medicago sativa  L. 
Metrosideros  
Morus  
Nerium oleander  L. 
Pluchea odorata  (L.) Cass.  
Polygala myrtifolia  L. 
Prunus  
Rhamnus alaternu s L. 
Rosmarinus officinalis  L. 
Rubus rigidus  Sm. 
Rubus ursinus  Cham. & Schldl.  
Sambucus   
Spartium junceum  L. 
Streptocarpus  
Teucrium capitatum  L. 
Ulmus americana  L. 
Vinca major  L. 
Vitis 
 
Specified plants susceptible to Xylella fastidiosa subspecies multiplex  
Acacia   
Acer griseum  (Franch.) Pax  
Acer pseudoplatanus  L. Acer rubrum  L. 
Alnus rhombifolia  Nutt.  
Ambrosia psilostachya  DC. 
Ambrosia trifida L.  
Ampelopsis cordata  Michx.  
Anthyllis hermanniae  L. 
Artemisia   
Asparagus acutifolius  L. 
Baccharis halimifolia  L. 
Calicotome spinosa  (L.) Link  
Calicotome villosa  (Poir.) Link  
Callistemon citrinus (Curtis) Skeels  
Calluna vulgaris  (L.) Hull  
Carya  
Celtis occidentalis  L. 
Cercis canadensis  L. 
Cercis  occidentalis  Torr.  
Cercis siliquastrum  L. 
Chionanthus  
Cistus  
Clematis cirrhosa L.  
Convolvulus cneorum  L. 
Coprosma repens  A. Rich.  
Coronilla glauca  (L.) Batt.  
Coronilla valentina  L. 
Cytisus  
Dimorphoteca  
Dodonaea  viscosa  (L.) Jacq.  
Elaeagnus angustif olia L. 
Encelia farinosa  Gray ex Torr.  
Erigeron karvinskianus  DC. 
Euryops chrysanthemoides  (DC.) B.Nord.  
Euryops pectinatus  (L.) Cass.  
Fallopia japonica  (Houtt.) Ronse Decr.  
Ficus carica  L. 
Frangula alnus  Mill.  
Fraxinus  
Genista  
Ginkgo biloba  L. 
Gleditsia triacanthos  L. 
Grevillea juniperina  Br. 
Hebe  Helianthus  
Helichrysum  
Ilex aquifolium  L. 
Iva annua  L. 
Koelreuteria bipinnata  Franch.  
Lagerstroemia  
Laurus nobilis  L. 
Lavandula  
Lavandula x intemedia  Emeric ex Loisel.  
Liquidambar styraciflua  L. 
Lupinus villosus  Willd.  
Medicago arborea  L. 
Medicago sativa  L. 
Metrosideros excelsa  Rich.  
Morus  
Myrtus communis  L. 
Olea  
Osteospermum ecklonis  (DC.) Norl.  
Pelargonium  
Phagnalon saxatile  (L.) Cass.  
Phillyrea angustifolia L. 
Phlomis fruticosa  L. 
Pistacia vera  L. 
Plantago lanceolata L. 
Platanus   
Polygala myrtifolia L. 
Polygala x grandiflora nana  
Prunus  
Pterospartum tridentatum (L.) Willk.  
Quercus  
Ratibida columnifera  (Nutt.) Wooton & Standl.  
Rhamnus alatern us L. 
Robinia pseudoacacia  L. 
Rosa  
Rosmarinus officinalis  L. 
Rubus  
Salvia mellifera Greene  
Sambucus  
Santolina chamaecyparissus  L. 
Sapindus sapona ria L. 
Solidago virgaurea  L. Spartium  
Strelitzia reginae  Aiton  
Ulex  
Ulmus americana  L. 
Ulmus crassifolia  Nutt.  
Vaccinium  
Vinca  
Westringia fruticos a Guerin.  
Xanthium strumarium  L. 
 
Specified plants susceptible to Xylella fastidiosa subspecies pauca  
Acacia  
Amaranthus retroflexus  L. 
Asparagus acutifolius  L. 
Catharanthus roseus  (L.) G. Don  
Chamaesyce canescens  (L.) Prokh.  
Chenopodium album  L. 
Cistus albidus  L. 
Cistus creticus  L. 
Citrus  
Coffea  
Dimorphoteca fruticosa  (L.) DC.  
Dodonaea viscosa  (L.) Jacq.  
Eremophila maculata  (Ker Gawler) F.vonMüll.  
Erigeron  
Euphorbia chamaesyce  L. 
Euphorbia terracina  L. 
Grevillea juniperina  Br. 
Hebe  
Heliotropium europaeum  L. 
Hibiscus  
Laurus nobilis  L. 
Lavandula angustifolia  Mill.  
Lavandula dentata  L. 
Lavandula stoechas  L. 
Myoporum insulare  Br. 
Myrtus communis  L. 
Nerium oleander  L. 
Olea europaea  L. 
Osteospermum fruticosum  (L.) Norl.  
Pelargonium x fragrans  Willd. (syn. Pelargonium fragrans E. Mey.)  Phillyrea latifolia  L. 
Polygala myrtifolia  L. 
Prunus  
Rhamnus alaternus  L. 
Rosmarinus officinalis  L. 
Spartium junceum  L. 
Vinca minor  L. 
Westringia fruticos a (Willd.) Druce  
Westringia glabra  Br. 
  ANNEX III  
Infected zones referred to in Article 4(2) wh ere containment measures set out in Articles 
13 to 17 are applied  
PART A  
Infected zone in Italy   
The infected zone of Italy includes the following areas:  
1. The province of Lecce  
2. The province of Brindisi  
3. Municipalities located in the province of Taranto:  
Avetrana  
Carosino  
Crispiano  
Faggiano  
Fragagnano  
Grottaglie  
Leporano  
Lizzano  
Manduria  
Martina Franca  
Maruggio  
Monteiasi  
Montemesola  
Monteparano  
Pulsano  
Roccaforzata  
San Giorgio Ionico  
San Marzano di San Giuseppe  
Sava  
Statte  
Taranto  
Torricella  
4. Municipality located in the province of Bari:  
Locorotondo  
 
 PART B  
Infected zone in France   
The infected zone in France includes the following area:  
The region of Corsica  
PART C  
Infected zone in Spain   
The infected zone in Spain includes the following area:  
The Autonomous Community of Balearic Islands  
  ANNEX IV  
Tests for the identification of Xylella fastidiosa  and its subspecies  
 
A. Tests for the screening and identification of the presence of Xylella fastidiosa   
1. Conventional PCR based on Minsavage et al. 1994 ; 
2. Loop -mediated isothermal amplification (LAMP) based on primers developed by 
Harper et al.  2010 (and erratum 2013);  
3. Real-time PCR based on Harper et al., 2010 (and erratum 2013);  
4. Real-time PCR based o n Li et al. , 2013;  
5. Real-time PCR based on Ouyang et al., 2013 . 
B. Molecular tests for the identification of the subspecies of Xylella fastidiosa   
1. Conventional PCR based on Hernandez -Martinez et al. , 2006 determining the 
subspecies fastidiosa , multiplex  and sandyi ;  
2. Conventional PCR based on Pooler & Hartung 1995 determining the subspecies  
pauca ; 
3. Multi Locus Sequence Typing (MLST) based on Yuan et al. , 2010 determining all 
subspecies .