DEAS 961: 2019 
ICS . 70 100.71 
 
© EAC 2019                                                                                                                              1st Edition 2019  
 DRAFT EAST AFRICAN STANDARD  
Glycerine for cosmetic industry  — Specification   
EAST AFRICAN COMMUNITY  DEAS 961: 2019  
ii © EAC  2019 – All rights reserved  
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© EAC 2019 – All rights reserved  iii 
 Foreword  
Development of the East African Standar ds has been necessitated by the need for harmonizing requirements 
governing quality of products and services in the East African Community. It is envisaged that through 
harmonized standardization, trade barriers that are encountered when goods and services  are exchanged 
within the Community will be removed.  
The Community has established an East African Standards Committee  (EASC)  mandated to develop and 
issue East African Standards  (EAS) . The Committee is composed of representatives of the National 
Standard s Bodies in Partner States, together with the representatives from the public and private sector 
organizations  in the community .  
East African Standards are developed through  Technical Committees that are representative of key 
stakeholders including govern ment, academia, consumer groups, private sector and other interested parties. 
Draft East African Standards are circulated to stakeholders through the National Standards Bodies in the 
Partner States. The comments received are discussed and incorporated befo re finalization of standards, in 
accordance with the Principles and procedures for development of East African Standards. XXXXXX.  
East African Standards are subject to review, to keep pace with technological advances. Users of the East 
African Standards ar e therefore expected to ensure that they always have the latest versions of the standards 
they are implementing .  
The committee responsible  for this document is Technical Committee EASC /TC 071, Cosmetics and related 
products . 
Attention is drawn to the poss ibility that some of the elements of this document may be subject of patent 
rights. EAC shall not be held responsible for identifying any or all such patent rights.  
 DRAFT EAST AFRICAN STANDARD  DEAS 961: 2019  
 
© EAC 2019 – All rights reserved  1 
 Glycerine  for cosmetic industry — Specification  
1 Scope   
This Draft East African Standard  specifies requirements, sampling and test methods for glycerine for cosmetic 
industry . 
2 Normative references  
The following referenced documents referred to in the text in such a way that some or all of their content 
constitutes requirements of this docu ment. For dated references, only the edition cited applies. For undated 
references, the latest edition of the referenced document (including any amendments) applies.  
EAS 846, Glossary of terms relating to the cosmetic industry  
EAS 847 -2, Oils for cosmetic industry — Methods of test — Part 2: Determination of moisture content  
EAS 847 -7, Oils for cosmetic industry — Methods of test — Part 7: Determination of specific gravity  
EAS 847 -15, Cosmetics — Analytical methods — Part 15: Determination  of ash content  
EAS 847 -16, Oils for cosmetic industry — Methods of test — Part 16: Determination of heavy metal content  
EAS 346 , Labelling of cosmetics — General requirements  
EAS 377 (all parts), Cosmetics and cosmetic products  
ISO 24153 , Random sampling and randomisation  procedures  
3 Terms and definitions  
For the purposes of this document, the terms and definitions given in EAS 846  and the following apply.  
ISO and IEC maintain terminological databases for use in standardization at the following addresses:  
— ISO Online browsing platform: available at http://www.iso.org/obp  
specific gravity  
ratio of the density of a material to the density of a standard material such as water (for liquids) or air (for 
gases)  
4 Requirements  
4.1 General requirements  
4.1.1 The raw material s hall comply with the requirements of all parts of EAS 377.  DEAS 961: 2019  
2 © EAC  2019 – All rights reserved  
 4.1.2 The product shall be:  
a) a clear syrupy liquid containing essentially of glycerol and having a sweet warm taste;  
b) free from  free from sediment, suspension and other foreign matter; and  
c) miscible i n all proportions with water and with ethyl alcohol (90 % v/v) and shall not be soluble in 
diethyl ether, chloroform  and fatty oils  
4.1.3  The product shall  be produced, prepared and handled in accordance with ISO 22716 . 
4.2 Specific quality requirements  
The product shall comply with the requirements given in Table 1 when tested in accordance with the test 
methods prescribed therein.  
Table 1 — Specific quality requirements for glycerine for cosmetic industry  
Characteristic  Requirement  Test method  
Glycerol,  % by mass, min.  98 Annex A  
Moisture content, % by mass, max.  2 EAS 847 - 2 
Specific gravity, at 25 °C  1.2490 - 1.2636  EAS 847 -7 
Ash, % by mass, max. 0.01 EAS 847 -15  
Alkalinity (as Na 2O), % by mass, max. 0.01 Annex B  
Chlorides (as Cl), mg/l, max. 10 Annex C  
Sulphates (as SO 4), mg/l, max. 10 Annex D  
Fatty acids and esters (as Na 2O), % by mass, 
max. 0.06 Annex E  
5 Heavy metal requirements  
Glycerine  shall comply with the heavy metal requirements given  in Table 2 when tested in accord ance with the 
test method s specif ied therein . 
Table 2 — Specific quality requirements for glycerine for cosmetic use  
Heavy metals,  Limit  
mg/l, max.  Test method  
Lead   5  
EAS 847 -16 Arsenic  2 
Mercury  2 
6 Pack aging 
The container (including the closure) in which the glyce rine is packaged shall not react chemically with the 
glycerine and shall be strong enough to protect the product adequately during handling, transportation and 
storage.  DEAS 961:2019  
© EAC 2019 – All rights reserved  3 
 7 Labelling  
In addition to the labelling requirements given  in EAS 346 , the package sha ll be legibly marked  with the 
following information:  
a) manufacturer's name and physical address;  
b) product name as “Glycerine”;  
c) net content of the product when packed;  
d) batch number;  
e) country of origin;  
f) instructions for use;  
g) date of manufacture and expiry; and  
h) precaution/warning , if any . 
8 Sampling  
Representative samples of the product shall be drawn randomly for test in accordance with ISO 24153 from 
the market, factory or elsewhere . DEAS 961: 2019  
4 © EAC  2019 – All rights reserved  
 Annex  A 
(normative)  
 
Determination of glycerol content  
A.1 Apparatus  
A.1.1  Burette  
A.1.2  Pipette  
A.1.3  Conical flasks with glass stopper , 500 -ml capacity  
A.1.4  Weighing pipette/bottle  
A.2 Reagents  
A.2.1  Sodium hydroxide solution , 0.1 N, accurately standardized  
A.2.2  Sodium hydroxide , 0.05 N 
A.2.3  Phenol red indicator , 0.05 % m/v.  Dissolve 0.05 g of phenol red in 10 ml of ethyl alcohol and make 
up to100 ml with water . 
A.2.4  Dilute sulphuric acid,  0.2 N  
A.2.5  Sodium metaperiodate solution . Dissolve 70 g of sodium metaperiodate in one litre of water 
containing 10 ml of 1 N sulphuric ac id and store the solution in an amber coloured bottle in the dark.Sodium 
metaperiodate shall be a white crystalline powder containing not less than 98 percent sodium metaperiodate.  
A.2.6  Ethylene glycol , neutral and free from glycerol  
A.2.7  Standard glycer ol  
A.3 Procedure  
A.3.1  Using a weighing pipette or bottle, transfer into a conical flask a well-mixed and accurately weighed  
sample.  
A.3.2  Add 100 ml of carbon dioxide -free water and three drops of phenol red indicator solution and acidify 
with sulphuric acid solution to definite yellow colour. Heat to boiling and cool to room temperature.  
A.3.3  Adjust the pH value to 8.0 ± 0.1 using 0.05 N sodium hydroxide solution dropwise until the colour 
changes to just pink. If the colour of the original solution inte rferes with the detection of colour change of the 
indicator, use a pH meter.  
A.3.4  Pipette accurately 50 ml of sodium metaperiodate solution, replace the glass stopper, mix by swirling 
gently and allow the flask to stand in the dark for 30 min.  DEAS 961:2019  
© EAC 2019 – All rights reserved  5 
 A.3.5  Wash down the sides of the flask with water, add 5 ml of ethylene glycol, replace the stopper, swirl 
gently and allow the flask to stand in the dark for further 20 min and titrate with standardized 0.05 N sodium 
hydroxide solution.  
A.3.6  Carry out a blank test simultaneously under similar test conditions . 
A.4 Calculation  
The glycerol content, expressed as percent by mass, shall be calculated as follows:  
MN) ( 9.209 BS
 
where  
S is the volume, in millilitres, of standard sodium hydroxide solution required for the sample;  
B is the volume, in millilitres, of standard sodium hydroxide solution required for the blank;  
N is the normality of standard sodium hydroxide solution; and  
M is the mass, in grams, of the sample taken for test.  
Each millilitre  of 0.05 N sodium hydroxide is equivalent to 9.210 mg of C 3H8O3. DEAS 961: 2019  
6 © EAC  2019 – All rights reserved  
 Annex B  
(normative)  
 
Test for alkalinity  
B.1 Apparatus  
B.1.1  Conical flask , 500 -ml capacity  
B.1.2  Burette  
B.2 Reagents  
B.2.1  Standardized hydrochloric acid , 0.1 N 
B.2.2  Phenolphthalein indicator solution  
B.3 Procedure  
B.3.1  Weigh accurately about 100 g of a well-mixed  sample into 500 -ml conical flask and add 150 ml of  
carbon dioxide -free water.  
B.3.2  Titrate with standardized 0.1 N hydrochloric acid using phenolphthalein indicator.  
B.4 Calculation  
The alkalinity (Na 2O), expressed as percent by mass, shall be calculated as follows:  
MNA3.1
 
where  
A is the volume, in millilitres, of standardized 0.1 N hydrochloric acid;  
N is the normality of standardized 0.1 N hydrochloric acid; and  
M is the  mass in grams of sample taken.  DEAS 961:2019  
© EAC 2019 – All rights reserved  7 
 Annex C  
(normative)  
 
Determination of chlorides  (Volumetric method)  
C.1 Apparatus  
Conical flasks , 250 -ml capacity  
C.2 Reagents  
C.2.1  Nitric acid solution , 35 % v/v  
C.2.2 Nitrobenzene  
C.2.3 Silver nitrate solution , 0.05 N  accurately standardized  
C.2.4 Ammonium thiocyanate solution , 0.05 N  accurately standardized  
C.2.5 Ferric ammonium sulphate solution , saturated, aqueous   
C.3 Procedure  
C.3.1  Weigh accurately about 20 g of the sample in a 250 -ml conical flask and add about 1 00 ml of water.  
C.3.2  To this solution, add 5 ml of nitric acid, a few drops of nitrobenzene and 5 ml of standard 0.05 N silver 
nitrate solution.  
C.3.3  Swirl and titrate with standard ized 0.05 N  ammonium thiocyanate solution using 1 ml of ferric 
ammonium  sulphate solution as indicator. Carry out a blank test using the same reagents.  
C.4 Calculation  
The chlorides (as Cl)  content , expressed as parts per million , shall be calculated as follows:  
MN) (50035 SB
 
where  
B is the volume , in millilitres, of standard ammonium thiocyanate solution  required for the blank ; 
S is the volume , in millilitres, of standard ammonium thiocyanate solution  required for the sample ; 
N is the normality of standard ammonium thiocyanate solution ; and 
M is the  mass , in grams,  of the material taken for the test.  DEAS 961: 2019  
8 © EAC  2019 – All rights reserved  
 Annex D  
(normative)  
 
Determination of sulphates  
D.1 Apparatus  
D.1.1  Nessler cylinders,  25-ml capacity  
D.1.2  Pipette, 1-ml capacity with 0.01 -ml graduations  
D.2 Reagents  
D.2.1 Hydrochloric acid , 1:1 solution of hydrochloric acid in water  
D.2.2 Barium chloride solution , 10 % (m/v), aqueous  
D.2.3 Standard sulphate solution . Dissolve 1.479 g of anhydrous sodium sulphate in water and make up 
to one litre in a volumetric flask. Dilute this stock solution  10 times immediately before use. One millilitre of this 
solution contains 0.000 1 g of sulphate ( SO 4). 
D.3 Procedure  
D.3.1  Weigh accurately about 10 g of the sample into a Nessler cylinder, add 1 ml of hydrochloric acid and 2 
ml of barium chloride solutio n, and make up to 25 ml with water. Allow to stand for 30 min. A dull white 
turbidity is produced if sulphates are present.  
D.3.2  Simultaneously, introduce 0.5 ml, 0.75 ml, 1.0 ml and 1.25 ml of standard sulphate solution into four 
separate Nessler cylinde rs. In each, add 1 ml of hydrochloric acid and 2 ml of barium chloride solution, and 
make up to 25 ml with water. Allow to stand for 30 min.  
D.3.3  Note which one of these four solutions has a turbidity most closely matching that produced by the test 
materi al (see D.3.1 ). Repeat the test and note the volume of standard sulphate solution required to produce a 
turbidity exactly matching that of the test sample.  
D.4 Calculation  
The sulphates (as SO 4) content , expressed as parts per million , shall be calculated as follows:  
MV100
 
where  
V is the volume , in millilitres, of standard sulphate solution used ; and 
M is the  mass , in grams,  of the material taken for the test.  DEAS 961:2019  
© EAC 2019 – All rights reserved  9 
 Annex E 
(normative)  
 
Determination of fatty acids and esters . 
E.1 Apparatus  
E.1.1 Round -bottomed flask , 500-ml capacity  
E.1.2  pH meter  
E.2 Reagents  
E.2.1 Sodium hydroxide solution , 0.25 N accurately standardized  
E.2.2 Sulphuric acid , 0 25 N accurately  standardized  
E.2.3 Phenolphthalein indicator solution  
E.3 Procedure  
E.3.1  Weigh a ccurately about 50 g of the sample into a 500 -ml round -bottomed flask. Add 100 ml of hot, 
carbon dioxide -free water and 1 ml of phenolphthalein indicator solution. If the solution is alkaline, neutralize it 
with sulphuric acid  
E.3.2  Add exactly 15.0 ml of  standard sodium hydroxide solution, connect the flask to a reflux condenser 
and heat to boiling. Boil for 5 min, allow to cool slightly and wash down the condenser with a little water. 
Disconnect the flask, close it with a stopper carrying a sodalime tube , and cool.  
E.3.3  Titrate with standardized 0.25 N sulphuric acid and perform a blank determination.  
E.4 Calculation  
The fatt y acids and esters  (as Na 2O) content , expressed as percent by mass , shall be calculated as follows:  
MNSB ) (3.1
 
where  
B is the volume , in millilitres, of standard sulphuric acid required for the  blank ; 
S is the volume , in millilitres, of standard sulphuric acid required for the  material ; 
N is the normality of standard sulphuric acid ; andM is the mass , in grams,  of the mat erial taken for the 
test. DEAS 961: 2019  
10 © EAC  2019 – All rights reserved  
 Bibliography  
[1] British pharmacopeia , 2015.  
[2] FDUS 1832:2018,  Glycerine for Cosmetic use 
[3] IS 1796 (1986): Glycerine  — Specification . 
[4] IS 12590 (1988): Glycerine for Cosmetic Industry . 
[5] Physical Properties of Glycerine  and Its Solutions , May 1967  
[6] USP 29 - NF 24 page 1011, pharmacopeial forum volume 28 (4) page 1245 0. 
 DEAS 961: 2019    
 
© EAC 2019 – All rights reserved