COMMISSION IMPLEMENTING REGUL ATION (EU) 2022/1266 
of 20 July 2022
concer ning the author isation of monosodium glut amate produced by fermenta tion with 
Cor ynebacter ium glutamicum KCCM 80187 as a feed additiv e for all animal species 
(Text with EEA relevance) 
THE EUR OPEAN COMMISSION,
Havi ng regar d to the Treaty on the Functioning of the European Union,
Havi ng rega rd to Regulation (EC) No 1831/2003 of the European Parliament and of the Council of 22 Sept ember 2003 on 
additives for use in animal nutr ition (1), and in particular Article 9(2) thereof,
Whereas:
(1) Regulation (EC) No 1831/2003 provid es for the author isation of additives for use in animal nutr ition and for the 
grounds and procedures for granting such author isation.
(2) In accordance with Article 7(1) of Regulation (EC) No 1831/2003 an application was submitte d for the 
author isation of monosodium glutamat e produced by fermentation with Corynebacter ium glutamicum KCCM 80187. 
That application was accompanied by the particulars and documents required under Article 7(3) of Regulation (EC) 
No 1831/2003.
(3) The application concer ns the author isation of monosodium glutamate as a feed additive for all animal species, to be 
classifie d in the categor y ‘sensor y additives ’ and in the functional group ‘flavouring compounds’.
(4) The applicant requested the additive to be author ised also for use in wate r for drinking. However , Regulation (EC) 
No 1831/2003 does not allow the author isation of ‘flavouri ng compounds’ for use in water for drinking. Theref ore, 
the use of monosodium glutamat e produced by fermentation with Corynebacter ium glutamicum KCCM 80187 in 
water for drinking should not be allowed.
(5) The European Food Safet y Author ity (‘the Auth ority’) concluded in its opinion of 10 Novem ber 2021 (2)that, under 
the proposed conditions of use, monosodium glutamat e produced by fermentation with Corynebacter ium glutamicum 
KCCM 80187 does not have adverse effects on animal health, human health or the environment. The Author ity 
concluded in the opinion that the additive is not toxic by inhalation, is not irritant to skin or eyes and is not a 
dermal sensitiser . The Authority further concluded, that monosodium glutamat e is efficacious for contr ibuting to 
the flavour of feed. The Auth ority also verified the repor t on the methods of analysis of the feed additive in feed 
submitted by the Reference Laboratory set up by Regulation (EC) No 1831/2003.
(6) The assessment of monosodium glutamat e produced by fermentation with Corynebacter ium glutamicum KCCM 80187 
shows that the conditions for author isation, as provid ed for in Article 5 of Regulation (EC) No 1831/2003, are 
satisfied. According ly, the use of that substance should be author ised as specifi ed in the Annex to this Regulation.
(7) Certain conditions should be provid ed for to allow better control. In particular , a recommended content should be 
indicated on the label of the feed additives. Where such content is exceeded, certain information should be indicated 
on the label of premixtures.
(8) The fact that monosodium glutamat e produced by fermentation with Corynebacter ium glutamicum KCCM 80187 is 
not author ised for use as a flavouring in water for drinking, does not preclude its use in compo und feed whic h is 
administe red via wate r.
(9) The measures provid ed for in this Regulation are in accordance with the opinion of the Standing Committ ee on 
Plants, Animals, Food and Feed,
(1)OJ L 268, 18.10.2003, p. 29.
(2)EFSA Jour nal 2021;19(12):6982.EN Offi cial Jour nal of the European Union 21.7.2022 L 192/17  HAS ADOPTED THIS REGUL ATION:
Article 1
The substance specified in the Annex, belonging to the additive cate gory ‘sensor y additives’ and to the functional group 
‘flavouring compounds’, is author ised as an additive in animal nutr ition, subject to the conditions laid down in that Annex.
Article 2
This Regulation shall enter into force on the twentieth day followi ng that of its publication in the Official Journal of the 
European Union.
This Regulation shall be binding in its entirety and directly applicable in all Member States.
Done at Brussels, 20 July 2022 .
For the Commission
The President
Ursula VON DER LEYENEN Offi cial Jour nal of the European Union L 192/18 21.7.2022  ANNEX 
Identifica -
tion 
number of 
the additiveAdditive Compo sition, chem ical formula, descr iption, analytical methodSpecies or 
categ ory of 
animalMaxi -
mum 
ageMini -
mum 
conten tMaxi -
mum 
conten t
Other provisi onsEnd of period of 
author isationmg active  
substance/kg of 
complet e feed 
with a moisture 
content of 12 %
Categor y: Sensor y additiv es. Functional group: Flavour ing compounds
2b621i Monosodium 
glutamateAdditive composition:
Monosodium glutamate
Char acter isation of the active substance:
Monosodium L-glut amate produced by fermentation with 
Corynebacter ium glutamicum KCCM 80187
Purity: ≥ 99 %
Chemical formula:
C5H8NaNO 4 •H 2O
CAS number: 6106-04-03
Einecs number: 205-538-1
Analytical method (1):
For the identification of monosodium L-glut amate in the feed 
additive:
—Food Chemical Codex ‘Monosodium L-glutamate mono ­
graph’
For the quantif ication of monosodium L-glutamat e in the feed 
additive:
— ion-ex chang e chromat ography coupled with post-column 
derivatisation and photometr ic detec tion (IEC- VIS)All animal 
species- - - 1.The additive shall be incor porat ed 
into the feed in the form of a pre­
mixture.
2.In the directions for use of the 
additive and premixtures, the sto­
rage conditions and stability to 
heat treatment shall be indicated .
3.On the label of the additive the 
follo wing shall be indicated:
‘Recommended maximum con­
tent of the active substance per 
kg of compl ete feeding stuff with 
a moisture conte nt of 12 %: 25 
mg.’
4.The functional group, the identi ­
fication number , the name and the 
added amount of the active sub­
stance shall be indicated on the 
label of the premixture where 
the use level on the label of the 
premixture would result in ex­
ceeding the level refer red to in 
point 3.10.8.2032EN Offi cial Jour nal of the European Union 21.7.2022 L 192/19  For the quantifi cation of monosodium L-glutamate in 
premixtures:
— ion-ex chang e chromat ography coupled with post-column 
derivatisation and photome tric detec tion (IEC- VIS), Com ­
mission Regulation (EC) No 152/2009 (Annex III, F)(2)
(1)Details of the analytical methods are available at the following address of the Reference Laborato ry: https://joint-research-c entre.ec.europa.eu/eurl-fa-eurl-f eed-additives/eurl-fa-author isation/eurl-f a-evaluation- 
repor ts_en
(2)Commission Regulation (EC) No 152/2009 of 27 Januar y 2009 laying down the methods of sampling and analysis for the official control of feed (OJ L 54, 26.2.2009, p. 1).EN Offi cial Jour nal of the European Union L 192/20 21.7.2022