126. Grant, A.; Ponia, S.S.; Tripathi, S.; Balasubramaniam, V.; Miorin, L.; Sourisseau, M.; Schwarz, M.C.; Sanchez-Seco, M.P.; Evans, M.J.; Best, S.M.; et al. Zika virus targets human Stat2 to inhibit type I interferon signaling. *Cell Host Microbe* **2016**, *19*, 882–890. [CrossRef] [PubMed]

127. Hertzog, J.; Dias Junior, A.G.; Rigby, R.E.; Donald, C.L.; Mayer, A.; Sezgin, E.; Song, C.; Jin, B.; Hublitz, P.; Eggeling, C.; et al. Infection with a Brazilian isolate of Zika virus generates RIG-I stimulatory RNA and the viral NS5 protein blocks type I IFN induction and signaling. *Eur. J. Immunol.* **2018**. [CrossRef] [PubMed]

128. Chaudhary, V.; Yuen, K.S.; Chan, J.F.; Chan, C.P.; Wang, P.H.; Cai, J.P.; Zhang, S.; Liang, M.; Kok, K.H.; Chan, C.P.; et al. Selective activation of type ii interferon signaling by Zika virus NS5 protein. *J. Virol.* **2017**, *91*. [CrossRef] [PubMed]

129. Nguyen, H.N.; Qian, X.; Song, H.; Ming, G.L. Neural stem cells attacked by zika virus. *Cell Res.* **2016**, *26*, 753–754. [CrossRef] [PubMed]

130. Slomnicki, L.P.; Chung, D.H.; Parker, A.; Hermann, T.; Boyd, N.L.; Hetman, M. Ribosomal stress and Tp53-mediated neuronal apoptosis in response to capsid protein of the Zika virus. *Sci. Rep.* **2017**, *7*, 16652. [CrossRef] [PubMed]

131. Yoon, K.J.; Song, G.; Qian, X.; Pan, J.; Xu, D.; Rho, H.S.; Kim, N.S.; Habela, C.; Zheng, L.; Jacob, F.; et al. Zika-virus-encoded NS2A disrupts mammalian cortical neurogenesis by degrading adherens junction proteins. *Cell Stem Cell* **2017**, *21*, 349–358 e346. [CrossRef] [PubMed]

132. Xie, D.Y.; Liu, Z.Y.; Nian, Q.G.; Zhu, L.; Wang, N.; Deng, Y.Q.; Zhao, H.; Ji, X.; Li, X.F.; Wang, X.; et al. A single residue in the alphab helix of the e protein is critical for Zika virus thermostability. *Emerg. Microbes Infect.* **2018**, *7*, 5. [CrossRef] [PubMed]

133. Brault, A.C.; Domi, A.; McDonald, E.M.; Talmi-Frank, D.; McCurley, N.; Basu, R.; Robinson, H.L.; Hellerstein, M.; Duggal, N.K.; Bowen, R.A.; et al. A zika vaccine targeting NS1 protein protects immunocompetent adult mice in a lethal challenge model. *Sci. Rep.* **2017**, *7*, 14769. [CrossRef] [PubMed]

134. Lei, J.; Hansen, G.; Nitsche, C.; Klein, C.D.; Zhang, L.; Hilgenfeld, R. Crystal structure of Zika virus NS2B-NS3 protease in complex with a boronate inhibitor. *Science* **2016**, *353*, 503–505. [CrossRef] [PubMed]

135. Bukrinsky, M. Yeast help identify cytopathic factors of zika virus. *Cell Biosci.* **2017**, *7*, 12. [CrossRef] [PubMed]

136. Nasmyth, K. A prize for proliferation. *Cell* **2001**, *107*, 689–701. [CrossRef]

137. Nurse, P. Cyclin dependent kinases and cell cycle control (nobel lecture). *Chembiochem* **2002**, *3*, 596–603. [CrossRef]

138. Tooze, S.A.; Dikic, I. Autophagy captures the Nobel Prize. *Cell* **2016**, *167*, 1433–1435. [CrossRef] [PubMed]

139. Zhao, R.Y. Yeast for virus research. *Microb. Cell* **2017**, *4*, 311–330. [CrossRef] [PubMed]

140. Zhao, Y.; Elder, R.T.; Chen, M.; Cao, J. Fission yeast expression vectors adapted for positive identification of gene insertion and green fluorescent protein fusion. *Biotechniques* **1998**, *25*, 438–440, 442, 444. [PubMed]

141. Li, G.; Zhao, R.Y. Molecular cloning and characterization of small viral genome in fission yeast. *Methods Mol. Biol.* **2018**, *1721*, 47–61. [PubMed]

142. Romero-Brey, I.; Bartenschlager, R. Endoplasmic reticulum: The favorite intracellular niche for viral replication and assembly. *Viruses* **2016**, *8*, 160. [CrossRef] [PubMed]

143. Kaufusi, P.H.; Kelley, J.F.; Yanagihara, R.; Nerurkar, V.R. Induction of endoplasmic reticulum-derived replication-competent membrane structures by West Nile virus non-structural protein 4B. *PLoS ONE* **2014**, *9*, e84040. [CrossRef] [PubMed]

144. Sangiambut, S.; Keelapang, P.; Aaskov, J.; Puttikhunt, C.; Kasinrerk, W.; Malasit, P.; Sittisombut, N. Multiple regions in dengue virus capsid protein contribute to nuclear localization during virus infection. *J. Gen. Virol.* **2008**, *89*, 1254–1264. [CrossRef] [PubMed]

145. Stock, N.K.; Escadafal, C.; Achazi, K.; Cisse, M.; Niedrig, M. Development and characterization of polyclonal peptide antibodies for the detection of yellow fever virus proteins. *J. Virol. Methods* **2015**, *222*, 110–116. [CrossRef] [PubMed]

146. Cox, B.D.; Stanton, R.A.; Schinazi, R.F. Predicting Zika virus structural biology: Challenges and opportunities for intervention. *Antivir. Chem. Chemother.* **2015**, *24*, 118–126. [CrossRef] [PubMed]

147. Kostyuchenko, V.A.; Lim, E.X.; Zhang, S.; Fibriansah, G.; Ng, T.S.; Ooi, J.S.; Shi, J.; Lok, S.M. Structure of the thermally stable zika virus. *Nature* **2016**, *533*, 425–428. [CrossRef] [PubMed]

148. Fontes-Garfias, C.R.; Shan, C.; Luo, H.; Muruato, A.E.; Medeiros, D.B.A.; Mays, E.; Xie, X.; Zou, J.; Roundy, C.M.; Wakamiya, M.; et al. Functional analysis of glycosylation of Zika virus envelope protein. *Cell Rep.* **2017**, *21*, 1180–1190. [CrossRef] [PubMed]
126. Grant, A.; Ponia, S.S.; Tripathi, S.; Balasubramaniam, V.; Miorin, L.; Sourisseau, M.; Schwarz, M.C.; Sanchez-Seco, M.P.; Evans, M.J.; Best, S.M.; et al. Zika virus targets human Stat2 to inhibit type I interferon signaling. *Cell Host Microbe* **2016**, *19*, 882–890. [CrossRef] [PubMed]

127. Hertzog, J.; Dias Junior, A.G.; Rigby, R.E.; Donald, C.L.; Mayer, A.; Sezgin, E.; Song, C.; Jin, B.; Hublitz, P.; Eggeling, C.; et al. Infection with a Brazilian isolate of Zika virus generates RIG-I stimulatory RNA and the viral NS5 protein blocks type I IFN induction and signaling. *Eur. J. Immunol.* **2018**. [CrossRef] [PubMed]

128. Chaudhary, V.; Yuen, K.S.; Chan, J.F.; Chan, C.P.; Wang, P.H.; Cai, J.P.; Zhang, S.; Liang, M.; Kok, K.H.; Chan, C.P.; et al. Selective activation of type ii interferon signaling by Zika virus NS5 protein. *J. Virol.* **2017**, *91*. [CrossRef] [PubMed]

129. Nguyen, H.N.; Qian, X.; Song, H.; Ming, G.L. Neural stem cells attacked by zika virus. *Cell Res.* **2016**, *26*, 753–754. [CrossRef] [PubMed]

130. Slomnicki, L.P.; Chung, D.H.; Parker, A.; Hermann, T.; Boyd, N.L.; Hetman, M. Ribosomal stress and Tp53-mediated neuronal apoptosis in response to capsid protein of the Zika virus. *Sci. Rep.* **2017**, *7*, 16652. [CrossRef] [PubMed]

131. Yoon, K.J.; Song, G.; Qian, X.; Pan, J.; Xu, D.; Rho, H.S.; Kim, N.S.; Habela, C.; Zheng, L.; Jacob, F.; et al. Zika-virus-encoded NS2A disrupts mammalian cortical neurogenesis by degrading adherens junction proteins. *Cell Stem Cell* **2017**, *21*, 349–358 e346. [CrossRef] [PubMed]

132. Xie, D.Y.; Liu, Z.Y.; Nian, Q.G.; Zhu, L.; Wang, N.; Deng, Y.Q.; Zhao, H.; Ji, X.; Li, X.F.; Wang, X.; et al. A single residue in the alphab helix of the e protein is critical for Zika virus thermostability. *Emerg. Microbes Infect.* **2018**, *7*, 5. [CrossRef] [PubMed]

133. Brault, A.C.; Domi, A.; McDonald, E.M.; Talmi-Frank, D.; McCurley, N.; Basu, R.; Robinson, H.L.; Hellerstein, M.; Duggal, N.K.; Bowen, R.A.; et al. A zika vaccine targeting NS1 protein protects immunocompetent adult mice in a lethal challenge model. *Sci. Rep.* **2017**, *7*, 14769. [CrossRef] [PubMed]

134. Lei, J.; Hansen, G.; Nitsche, C.; Klein, C.D.; Zhang, L.; Hilgenfeld, R. Crystal structure of Zika virus NS2B-NS3 protease in complex with a boronate inhibitor. *Science* **2016**, *353*, 503–505. [CrossRef] [PubMed]

135. Bukrinsky, M. Yeast help identify cytopathic factors of zika virus. *Cell Biosci.* **2017**, *7*, 12. [CrossRef] [PubMed]

136. Nasmyth, K. A prize for proliferation. *Cell* **2001**, *107*, 689–701. [CrossRef]

137. Nurse, P. Cyclin dependent kinases and cell cycle control (nobel lecture). *Chembiochem* **2002**, *3*, 596–603. [CrossRef]

138. Tooze, S.A.; Dikic, I. Autophagy captures the Nobel Prize. *Cell* **2016**, *167*, 1433–1435. [CrossRef] [PubMed]

139. Zhao, R.Y. Yeast for virus research. *Microb. Cell* **2017**, *4*, 311–330. [CrossRef] [PubMed]

140. Zhao, Y.; Elder, R.T.; Chen, M.; Cao, J. Fission yeast expression vectors adapted for positive identification of gene insertion and green fluorescent protein fusion. *Biotechniques* **1998**, *25*, 438–440, 442, 444. [PubMed]

141. Li, G.; Zhao, R.Y. Molecular cloning and characterization of small viral genome in fission yeast. *Methods Mol. Biol.* **2018**, *1721*, 47–61. [PubMed]

142. Romero-Brey, I.; Bartenschlager, R. Endoplasmic reticulum: The favorite intracellular niche for viral replication and assembly. *Viruses* **2016**, *8*, 160. [CrossRef] [PubMed]

143. Kaufusi, P.H.; Kelley, J.F.; Yanagihara, R.; Nerurkar, V.R. Induction of endoplasmic reticulum-derived replication-competent membrane structures by West Nile virus non-structural protein 4B. *PLoS ONE* **2014**, *9*, e84040. [CrossRef] [PubMed]

144. Sangiambut, S.; Keelapang, P.; Aaskov, J.; Puttikhunt, C.; Kasinrerk, W.; Malasit, P.; Sittisombut, N. Multiple regions in dengue virus capsid protein contribute to nuclear localization during virus infection. *J. Gen. Virol.* **2008**, *89*, 1254–1264. [CrossRef] [PubMed]

145. Stock, N.K.; Escadafal, C.; Achazi, K.; Cisse, M.; Niedrig, M. Development and characterization of polyclonal peptide antibodies for the detection of yellow fever virus proteins. *J. Virol. Methods* **2015**, *222*, 110–116. [CrossRef] [PubMed]

146. Cox, B.D.; Stanton, R.A.; Schinazi, R.F. Predicting Zika virus structural biology: Challenges and opportunities for intervention. *Antivir. Chem. Chemother.* **2015**, *24*, 118–126. [CrossRef] [PubMed]

147. Kostyuchenko, V.A.; Lim, E.X.; Zhang, S.; Fibriansah, G.; Ng, T.S.; Ooi, J.S.; Shi, J.; Lok, S.M. Structure of the thermally stable zika virus. *Nature* **2016**, *533*, 425–428. [CrossRef] [PubMed]

148. Fontes-Garfias, C.R.; Shan, C.; Luo, H.; Muruato, A.E.; Medeiros, D.B.A.; Mays, E.; Xie, X.; Zou, J.; Roundy, C.M.; Wakamiya, M.; et al. Functional analysis of glycosylation of Zika virus envelope protein. *Cell Rep.* **2017**, *21*, 1180–1190. [CrossRef] [PubMed]
149. Annamalai, A.S.; Pattnaik, A.; Sahoo, B.R.; Muthukrishnan, E.; Natarajan, S.K.; Steffen, D.; Vu, H.L.X.; Delhon, G.; Osorio, F.A.; Petro, T.M.; et al. Zika virus encoding non-glycosylated envelope protein is attenuated and defective in neuroinvasion. *J. Virol.* **2017**, *91*. [CrossRef] [PubMed]

150. Goo, L.; DeMaso, C.R.; Pelc, R.S.; Ledgerwood, J.E.; Graham, B.S.; Kuhn, R.J.; Pierson, T.C. The Zika virus envelope protein glycan loop regulates virion antigenicity. *Virology* **2018**, *515*, 191–202. [CrossRef] [PubMed]

151. Gallichotte, E.N.; Dinnon, K.H., 3rd; Lim, X.N.; Ng, T.S.; Lim, E.X.Y.; Menachery, V.D.; Lok, S.M.; Baric, R.S. CD-loop extension in Zika virus envelope protein key for stability and pathogenesis. *J. Infect. Dis.* **2017**, *216*, 1196–1204. [CrossRef] [PubMed]

152. Yuan, L.; Huang, X.Y.; Liu, Z.Y.; Zhang, F.; Zhu, X.L.; Yu, J.Y.; Ji, X.; Xu, Y.P.; Li, G.; Li, C.; et al. A single mutation in the prm protein of Zika virus contributes to fetal microcephaly. *Science* **2017**, *358*, 933–936. [CrossRef] [PubMed]

153. Shan, C.; Xie, X.; Muruato, A.E.; Rossi, S.L.; Roundy, C.M.; Azar, S.R.; Yang, Y.; Tesh, R.B.; Bourne, N.; Barrett, A.D.; et al. An infectious cdna clone of Zika virus to study viral virulence, mosquito transmission, and antiviral inhibitors. *Cell Host Microbe* **2016**, *19*, 891–900. [CrossRef] [PubMed]

154. Mahawaththa, M.C.; Pearce, B.J.G.; Szabo, M.; Graham, B.; Klein, C.D.; Nitsche, C.; Otting, G. Solution conformations of a linked construct of the Zika virus NS2B-NS3 protease. *Antiviral Res.* **2017**, *142*, 141–147. [CrossRef] [PubMed]

155. Phoo, W.W.; Li, Y.; Zhang, Z.; Lee, M.Y.; Loh, Y.R.; Tan, Y.B.; Ng, E.Y.; Lescar, J.; Kang, C.; Luo, D. Structure of the NS2B-NS3 protease from Zika virus after self-cleavage. *Nat. Commun.* **2016**, *7*, 13410. [CrossRef] [PubMed]

156. Lee, H.; Ren, J.; Nocadello, S.; Rice, A.J.; Ojeda, I.; Light, S.; Minasov, G.; Vargas, J.; Nagarathnam, D.; Anderson, W.F.; et al. Identification of novel small molecule inhibitors against NS2B/NS3 serine protease from zika virus. *Antiviral Res.* **2017**, *139*, 49–58. [CrossRef] [PubMed]

157. Shafee, N.; AbuBakar, S. Dengue virus type 2 NS3 protease and NS2B-NS3 protease precursor induce apoptosis. *J. Gen. Virol.* **2003**, *84*, 2191–2195. [CrossRef] [PubMed]

158. Yang, T.C.; Shiu, S.L.; Chuang, P.H.; Lin, Y.J.; Wan, L.; Lan, Y.C.; Lin, C.W. Japanese encephalitis virus NS2B-NS3 protease induces caspase 3 activation and mitochondria-mediated apoptosis in human medulloblastoma cells. *Virus Res.* **2009**, *143*, 77–85. [CrossRef] [PubMed]

159. Rastogi, M.; Sharma, N.; Singh, S.K. Flavivirus ns1: A multifaceted enigmatic viral protein. *Virol. J.* **2016**, *13*, 131. [CrossRef] [PubMed]

160. Viranaicken, W.; Ndebo, A.; Bos, S.; Souque, P.; Gadea, G.; El-Kalamouni, C.; Krejbich-Trotot, P.; Charneau, P.; Despres, P.; Roche, M. Recombinant zika ns1 protein secreted from vero cells is efficient for inducing production of immune serum directed against NS1 dimer. *Int. J. Mol. Sci.* **2017**, *19*, 38. [CrossRef] [PubMed]

161. Freire, C.C.M.; Lamarino, A.; Neto, D.F.L.N.; Sall, A.A.; Zanotto, P.M.A. Spread of the pandemic Zika virus lineage is associated with NS1 codon usage adaptation in humans. *BioRxiv* **2015**. [CrossRef]

162. Delatorre, E.; Mir, D.; Bello, G. Tracing the origin of the NS1 A188V substitution responsible for recent enhancement of Zika virus Asian genotype infectivity. *Mem. Inst. Oswaldo Cruz* **2017**, *112*, 793–795. [CrossRef] [PubMed]

<figure><img src="image_6.png" /></figure>
149. Annamalai, A.S.; Pattnaik, A.; Sahoo, B.R.; Muthukrishnan, E.; Natarajan, S.K.; Steffen, D.; Vu, H.L.X.; Delhon, G.; Osorio, F.A.; Petro, T.M.; et al. Zika virus encoding non-glycosylated envelope protein is attenuated and defective in neuroinvasion. *J. Virol.* **2017**, *91*. [CrossRef] [PubMed]

150. Goo, L.; DeMaso, C.R.; Pelc, R.S.; Ledgerwood, J.E.; Graham, B.S.; Kuhn, R.J.; Pierson, T.C. The Zika virus envelope protein glycan loop regulates virion antigenicity. *Virology* **2018**, *515*, 191–202. [CrossRef] [PubMed]

151. Gallichotte, E.N.; Dinnon, K.H., 3rd; Lim, X.N.; Ng, T.S.; Lim, E.X.Y.; Menachery, V.D.; Lok, S.M.; Baric, R.S. CD-loop extension in Zika virus envelope protein key for stability and pathogenesis. *J. Infect. Dis.* **2017**, *216*, 1196–1204. [CrossRef] [PubMed]

152. Yuan, L.; Huang, X.Y.; Liu, Z.Y.; Zhang, F.; Zhu, X.L.; Yu, J.Y.; Ji, X.; Xu, Y.P.; Li, G.; Li, C.; et al. A single mutation in the prm protein of Zika virus contributes to fetal microcephaly. *Science* **2017**, *358*, 933–936. [CrossRef] [PubMed]

153. Shan, C.; Xie, X.; Muruato, A.E.; Rossi, S.L.; Roundy, C.M.; Azar, S.R.; Yang, Y.; Tesh, R.B.; Bourne, N.; Barrett, A.D.; et al. An infectious cdna clone of Zika virus to study viral virulence, mosquito transmission, and antiviral inhibitors. *Cell Host Microbe* **2016**, *19*, 891–900. [CrossRef] [PubMed]

154. Mahawaththa, M.C.; Pearce, B.J.G.; Szabo, M.; Graham, B.; Klein, C.D.; Nitsche, C.; Otting, G. Solution conformations of a linked construct of the Zika virus NS2B-NS3 protease. *Antiviral Res.* **2017**, *142*, 141–147. [CrossRef] [PubMed]

155. Phoo, W.W.; Li, Y.; Zhang, Z.; Lee, M.Y.; Loh, Y.R.; Tan, Y.B.; Ng, E.Y.; Lescar, J.; Kang, C.; Luo, D. Structure of the NS2B-NS3 protease from Zika virus after self-cleavage. *Nat. Commun.* **2016**, *7*, 13410. [CrossRef] [PubMed]

156. Lee, H.; Ren, J.; Nocadello, S.; Rice, A.J.; Ojeda, I.; Light, S.; Minasov, G.; Vargas, J.; Nagarathnam, D.; Anderson, W.F.; et al. Identification of novel small molecule inhibitors against NS2B/NS3 serine protease from zika virus. *Antiviral Res.* **2017**, *139*, 49–58. [CrossRef] [PubMed]

157. Shafee, N.; AbuBakar, S. Dengue virus type 2 NS3 protease and NS2B-NS3 protease precursor induce apoptosis. *J. Gen. Virol.* **2003**, *84*, 2191–2195. [CrossRef] [PubMed]

158. Yang, T.C.; Shiu, S.L.; Chuang, P.H.; Lin, Y.J.; Wan, L.; Lan, Y.C.; Lin, C.W. Japanese encephalitis virus NS2B-NS3 protease induces caspase 3 activation and mitochondria-mediated apoptosis in human medulloblastoma cells. *Virus Res.* **2009**, *143*, 77–85. [CrossRef] [PubMed]

159. Rastogi, M.; Sharma, N.; Singh, S.K. Flavivirus ns1: A multifaceted enigmatic viral protein. *Virol. J.* **2016**, *13*, 131. [CrossRef] [PubMed]

160. Viranaicken, W.; Ndebo, A.; Bos, S.; Souque, P.; Gadea, G.; El-Kalamouni, C.; Krejbich-Trotot, P.; Charneau, P.; Despres, P.; Roche, M. Recombinant zika ns1 protein secreted from vero cells is efficient for inducing production of immune serum directed against NS1 dimer. *Int. J. Mol. Sci.* **2017**, *19*, 38. [CrossRef] [PubMed]

161. Freire, C.C.M.; Lamarino, A.; Neto, D.F.L.N.; Sall, A.A.; Zanotto, P.M.A. Spread of the pandemic Zika virus lineage is associated with NS1 codon usage adaptation in humans. *BioRxiv* **2015**. [CrossRef]

162. Delatorre, E.; Mir, D.; Bello, G. Tracing the origin of the NS1 A188V substitution responsible for recent enhancement of Zika virus Asian genotype infectivity. *Mem. Inst. Oswaldo Cruz* **2017**, *112*, 793–795. [CrossRef] [PubMed]

<figure><img src="image_6.png" /></figure>

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