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Sep 04, 2020

# INDICAID Rapid Point-of-Care Test for SARS-CoV-2 Antigen

DOI

https://dx.doi.org/10.17504/protocols.io.bkt4kwqw

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Phase Diagnostics¹

¹Phase Diagnostics

Coronavirus Method De...

Phase Diagnostics

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Phase Diagnostics

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DOI: https://dx.doi.org/10.17504/protocols.io.bkt4kwqw

**Protocol Citation:** Phase Diagnostics 2020. INDICAID Rapid Point-of-Care Test for SARS-CoV-2 Antigen. protocols.io
https://dx.doi.org/10.17504/protocols.io.bkt4kwqw

**License:** This is an open access protocol distributed under the terms of the **Creative Commons Attribution License**, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
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**Protocol status:** In development

We are still developing and optimizing this protocol

**Created:** September 04, 2020

**Last Modified:** September 05, 2020

**Protocol Integer ID:** 41564

**Keywords:** care test for sar, sar, antigen, care test, indicaid rapid point

# Materials

## MATERIALS

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Dropper Vial Cangzhou Shengfeng Plastic Product Co. Catalog #DB-4ML

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1.5ml microcentrifuge tube GenFollower Catalog #WG00012

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Nasal Swab Puritan Catalog #25-806 1PR

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Concentration Buffer

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Sample Buffer

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Test Cassette

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Transfer Pipette Globe Scientific Catalog #139118

# Troubleshooting
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Test Procedure

1 The INDICAID Rapid Point-of-Care Test for SARS-CoV-2 Antigen includes swabs for nasal specimen collection.

2 Insert the swab into one of the nostrils until a slight resistance is met (approx. 1 cm from edge of nostril). Roll the swab for 00:00:05 seconds inside the nostril to ensure that both cells and mucus are collected.

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3 Remove the swab from the nostril and repeat on the other nostril using the same swab.

4 The Collection Vial cap is composed of two parts. Remove the entire cap.

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5 Insert the swab so that it is completely submerged in the buffer.
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6 While submerged, twist the swab 20 times to release the specimen into the buffer.

7 Discard the swab and close the cap on the Collection Vial tightly. Let it sit for 00:01:00 minute.

8 Remove the small cap (top half only) from the Collection Vial to expose the dropper tip.

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9 Squeeze ALL contents of the Collection Vial into the Concentrating Vial.
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10. Close the cap of the Concentrating Vial and centrifuge

the vial at ⏻ 9000 rpm, 00:02:00

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Equipment

<table><tr><td>Multi Speed Mini Centrifuge</td><td>NAME</td></tr><tr><td>Centrifuge</td><td>TYPE</td></tr><tr><td>HWLAB</td><td>BRAND</td></tr><tr><td>L-2101</td><td>SKU</td></tr></table>

http://www.hwlab.cn/product/single_multi_speed.htmlLINK

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11 After centrifuging, carefully open the Concentrating Vial and look for the clear bottom liquid layer. Fill the provided clean fixed volume pipette with 80 µL of the clear bottom layer.

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12. Apply all the contents of the pipette into the Test Cassette sample well.

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13. Read the result in the result window of the test device after 00:15:00 minutes.

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