# Delivery of SiC-based nanoparticles into live cells driven by cell-penetrating peptides SAP and SAP-E

T. Serdiuk, I. Bakanovich, Vladimir Lysenko, S. Alekseev, V. Skryshevsky, S.

Afonin, Eric Berger, Alain Geloen, I. Komarov

► To cite this version:

T. Serdiuk, I. Bakanovich, Vladimir Lysenko, S. Alekseev, V. Skryshevsky, et al., Delivery of SiC-based nanoparticles into live cells driven by cell-penetrating peptides SAP and SAP-E. RSC Advances, 2015, 5 (26), pp.20498-20502. 10.1039/C4RA10688A. hal-01489394

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Distributed under a Creative Commons Attribution 4.0 International License
Delivery of SiC-based nanoparticles into live cells
driven by cell-penetrating peptides SAP and SAP-E

T. Serdiuk,<sup>ab</sup> I. Bakanovich,<sup>c</sup> V. Lysenko,<sup>d</sup> S. A. Alekseev,<sup>b</sup> V. A. Skryshevsky,<sup>b</sup> S. Afonin,<sup>e</sup>
E. Berger,<sup>a</sup> A. G'elo"en<sup>a</sup> and I. V. Komarov*<sup>b</sup>

The delivery of SiC-based nanoparticles (SiC-NPs) into living eukary-
otic cells is facilitated in the presence of cell-penetrating peptides,
both cationic (SAP) and anionic (SAP-E). The SiC-NP surface functional
group modification combined with rational CPP selection introduces
an additional mode of delivery control.

Nanometer-sized semiconductor particles (NPs)¹⁻⁵ have aroused
considerable interest of researchers in the last decade, primarily
due to their promising biomedical applications.¹,² Their fluo-
rescent properties have already found use in modern imaging
technologies,⁶⁻¹⁰ providing the opportunity to visualize biolog-
ical processes by fluorescence labelling at the tissue,¹¹ cellular¹²
and single-molecular¹³ levels. Compared to other known fluo-
rophores, NPs have higher quantum yields and molecular
extinction coefficients of the fluorescence, narrower emission
spectra, size-dependent emission, and higher chemical and
photo-stability.¹⁴,¹⁵

The main problems that need to be addressed in the fluo-
rescence labelling using NPs are the inefficient penetration of
the NPs through cell membranes and their non-specific delivery
inside the cells. Many studies were devoted to develop various
strategies for NP internalization into the living cells, with
special emphasis on the selective targeting of the NPs to
different cellular organelles and compartments.¹⁶ Most of the
reported delivering strategies were based on the conjugation of
the NPs to biomolecules,¹⁶,¹⁷ which might also drive them to

specific cellular locations. The use of cell-penetrating peptides (CPPs) as carrier molecules is of the particular interest in this respect. The CPPs linked to nano-cargoes efficiently promote cargo internalization without significant cytotoxic effects,¹⁸ and in many cases, they were shown to target specific cellular organelles like nuclei or mitochondria.¹⁹ Therefore, the CPPs can not only facilitate the NP uptake by cells, but also deliver them to specific sites inside the cells.²⁰⁻²²

Among the numerous NP types reported to date, ZnS-covered CdSe quantum dots (CdSe@ZnS QDs) are probably the most studied ones. Important advances in their preparation<sup>23-25</sup> and conjugation to different biomolecules, including CPPs,<sup>16</sup> have led to spectacular bioimaging applications. However, other NP types that might offer advantages in comparison with the CdSe@ZnS QDs are not studied much; obviously, their potential has to be explored as well. We disclose in this study our results of such an exploration into the intra-cellular delivery of NPs prepared by the electrochemical anodization of a low resistivity grade (&lt;1 Ω cm) bulk 3C-SiC polycrystalline wafer (SiC-based NPs, SiC-NPs<sup>‡</sup>).<sup>26</sup> Using this type of NPs for bioimaging application, one can benefit from the simplicity of their preparation procedures, low potential toxicity,<sup>27,28</sup> as well as from their ability to form stable colloidal solutions in water and other polar solvents.

Recently, the charge-driven selective localization of fluores-
cent SiC-NPs in cells was reported.²⁹ The manipulation of the
SiC-NP surface functional groups was shown to actively govern
the intracellular fate of the SiC-NPs. The as-prepared SiC-NPs,
possessing carboxylic groups on their surface,³⁰ are deproto-
nated and charged negatively at physiological pH (SiC-NPs(−),
Fig. 1). The reaction shown in Fig. 1a transformed the carboxylic
moieties into positively charged aminoethylcarboxamide func-
tional groups, protonated under the physiological conditions
(SiC-NPs(+), Fig. 1b).²⁹ The negatively charged SiC-NPs(−)
targeted the cell nuclei; moreover, under the same conditions,
the positively charged SiC-NPs(+) were unable to penetrate the
nuclei and accumulated in the cytosol. The excellent fluores-
cence of the SiC-NPs(+) and SiC-NPs(−) (Fig. 1c) allowed the

<footnote><sup>a</sup>University of Lyon, CarMeN Laboratory, INSA de Lyon, UMR INSERM 1060, France</footnote>

<footnote><sup>b</sup>Taras Shevchenko National University of Kyiv, Volodymyr’ka 64, 01601 Kyiv,
Ukraine. E-mail: ik214@yahoo.com</footnote>

<footnote>Enamine Ltd, Matrosova 23, 01103 Kyiv, Ukraine</footnote>

<footnote><sup>d</sup>University of Lyon, Nanotechnology Institute of Lyon (INL), INSA de Lyon, UMR CNRS 5270, France</footnote>

<footnote>Institute of Biological Interfaces (IBG-2), Karlsruhe Institute of Technology, POB 3640,
76021 Karlsruhe, Germany</footnote>
efficient visualization of the cells treated by the NPs of this type using a fluorescence microscope.

Targeting the cell nuclei by the as-prepared negatively charged SiC-NPs(−) was shown to be closely associated with the process of cell division. In the normal fibroblast or epithelial cells, the overall uptake of SiC-NPs(−) and their accumulation in the nuclei are both markedly reduced when cells are confluent and do not divide with no nucleus targeting at the full confluence state.³¹

In this study, we report on an improved strategy to achieve the efficient and selective internalization of the SiC-NPs into live cells, which is based on the CPP-assisted delivery. This strategy can be applied to both normally dividing and confluent cells. For each given surface modification of the SiC-NPs, we propose to choose the corresponding carrier CPP, selecting it from a large known pool,¹⁸ so that the SiC-NP surface functional groups could form non-covalent bonds with the complementary side-chain groups of the CPP and are therefore “matched”. In particular, amphiphilic arginine-containing CPP “Sweet Arrow Peptide” (SAP, (Val-Arg-Leu-Pro-Pro-Pro)₃)³²,³³ and its analogue, glutamate-containing SAP-E (Val-Glu-Leu-Pro-Pro-Pro)₃)³⁴ were chosen to be used as the carrier peptides. We chose them because they possess differently charged side chains (positively charged SAP and negatively charged SAP-E); both cationic SAP and anionic SAP-E were artificially designed and reported to

efficiently internalize into eukaryotic cells.³¹⁻³³ In accordance with our “matching” hypothesis, the positively charged SAP can form non-covalent conjugates with the negatively charged SiC-NPs(−). Coulombic attraction between the charged groups should be reinforced in this case by the hydrogen bonds between the carboxylic and guanidinium moieties (Fig. 2a), which is important for the CPP to compete efficiently for the binding sites with the “protein corona” of the nanoparticles formed in living systems.³⁵⁻³⁷ Analogously, the anionic SAP-E can interact with the positively charged SiC-NPs(+), as schematically shown in the Fig. 2b. Importantly, the SAP and SAP-E peptides were purposely designed as amphipathic PPII helices,³¹⁻³³ so their charged functional groups are located at one side of their molecules, positioned to form multiple non-covalent bonds with one nanoparticle.

Only a small fraction of the peptide might interact reversibly with the NP surface, due to the non-covalent nature of the interaction. The indirect indication of this is the zeta-potential of the nanoparticles, which changed only slightly upon the peptide addition (for example, from +100 to +97 mV in the SiC-NPs(+)/SAP-E system). However, this might be sufficient to affect the transport of the NPs through the cell membrane.

The experimental procedure for the (SiC-NPs)/(carrier peptide) conjugation, as it relied upon the non-covalent interactions, was very simple. The corresponding nanoparticle solutions were just mixed with the SAP or SAP-E solutions and kept at room temperature for several hours (see ESI† for the details). The cell cultures of 3T3-L1 murine fibroblasts (American Type Culture Collection, Manassas, VA, USA) were exposed to the formed solutions overnight. The fluorescence of the cell cultures incubated with the NP solutions and that of the non-treated (control) cells was observed by means of a fluorescence microscope, and increase in the fluorescence over the background in the presence of the NPs alone or their complexes with the CPPs in different combinations was determined. The results are illustrated in Fig. 3.

As can be seen from Fig. 3, the treatment of the SiC-NPs by a carrier peptide indeed facilitated the intracellular entry of the nanoparticles, but only for certain SiC-NPs/carrier combinations, in agreement to our “matching” hypothesis. For example, SiC-NPs(−) treated by SAP increased the fluorescence of the cells higher than that observed upon treatment with the SiC-NPs(−) alone, as can be judged from the average fluorescence per cell counting (compare the Fig. 3a–e, and see the ESI†). The most pronounced effect of the CPP presence was observed when the cells were at full confluence: the non-proliferating confluent cells were efficiently labelled by the SiC-NPs(−) incubated with SAP (Fig. 3e), contrary to the as-prepared peptide-free SiC-NPs(−) (Fig. 3d). Therefore, the presence of SAP conferred the SiC-NP(−) the ability to enter densely grown non-dividing cells, and increased the overall cell entry of the particles. In addition, the CPP-driven delivery of the SiC-NPs(−) into the proliferating living cells was nuclei-selective, as can be seen in Fig. 3e (inset). Similar selectivity was previously observed²⁹ when the peptide-free SiC-NPs(−) penetrated inside the nuclei of the non-confluent cells (Fig. 3a).

<figure><img src="image_2.png" /><figcaption>Fig. 1 Chemical modification of the carboxylic groups on the surface of the SiC-NPs (a), their ionization states under cellular internalization conditions (b), and photoluminescence spectra (excitation at 343 nm) of the as-prepared SiC-NPs used in this study (c).</figcaption></figure>
<figure><img src="image_3.png" /><figcaption>Fig. 2 Expected non-covalent interactions between oppositely charged SiC-NPs and CPPs. The as-prepared anionic SiC-NP(−) interacting with arginine side chains of SAP (a); and cationic SiC-NP(+) interacting with glutamate residues of SAP-E (b).</figcaption></figure>

The enhancement of the intracellular SiC-NPs(+) delivery was observed when the nanoparticles were treated by SAP-E (compare Fig. 3g and i, j and l). Drastic difference in the imaging ability between the SiC-NPs(+) alone and the SiC-NPs(+)/SAP-E pair was noticed: while the bare SiC-NPs(+)

stayed predominantly in the cytosol (Fig. 3g), the nanoparticles treated with SAP-E accumulated exclusively in the cell nuclei (Fig. 3i). This difference cannot be explained simply by recharging the SiC-NPs(+) in the presence of SAP-E: as we noticed above, the zeta-potential of the SiC-NPs(+)/SAP-E

<figure><img src="image_4.png" /><figcaption>Fig. 3 Fluorescent microscopic images of the 3T3-L1 cells (normally dividing and in a confluent state) exposed to SiC-NPs(−) alone (a and d); SiC-NPs(+) alone (g and j); treated with the SiC-NPs(−)/SAP complex (b and e); treated with the SiC-NPs(+)/SAP complex (h and k), treated with the SiC-NPs(−)/SAP-E complex (c and f); treated with the SiC-NPs(+)/SAP-E complex (i and l). Insets are parts of the corresponding images magnified by 2. The enhancement of the fluorescence intensity over the background (in percent) is shown under each image.</figcaption></figure>
complex (+97 mV) was only slightly lower than that of the as-
prepared SiC-NPs(+) (+100 mV). Obviously, the CPP-induced
NPs intracellular delivery should be explained by a different
mechanism than in the case of NPs without SAP.

Most importantly, while the SiC-NPs(-)/SAP complex
penetrated efficiently into the confluent cells (see Fig. 3e, the
CPP-cargo pair is "matched"), the incubation of the cell
cultures close to the confluent state in the presence of the
SiC-NPs(+)/SAP complexes did not lead to significant fluores-
cent labelling (Fig. 3k), most probably because stable conju-
gates did not form in this case (the CPP-cargo pair is
"mismatched"). Obviously, in order to achieve efficient NPs
cell delivery, the choice of the carrier peptide is highly
important. It should consider the SiC-NPs functional groups,
their ionization state, as well as the nature of the peptide side
chains.

It is also important to note that SAP, SAP-E and their
complexes with the SiC-NPs in different combinations at the
concentration of 0.4 mg mL⁻¹ had no significant effect on the
cell proliferation and did not induce cell death. This was
confirmed by real-time cell analysis (see the ESI†). We also
measured the gene expression of the specific cell markers of
stress, cell death, and inflammation. In all the cases the
transcription of the selection of genes specifically activated by
apoptosis, inflammation, mitochondrial stress or unfolded
protein response did not significantly modulate in response
to SAP or SAP-E at 0.4 mg mL⁻¹. This indicated that the cells
were perfectly safe under the experimental conditions, and
the SAP/SAP-E did not induce significant traumatic shock to
the cells.

Conclusions

Cell-penetrating peptides SAP and SAP-E are efficient tools for controlled fluorescent cell labelling by the SiC-NPs. The labelling of the cells is simple and practical, not dependent on the cell state, thus it can be recommended for bioimaging applications in live cell cultures. The cationic SAP is most suitable for the delivery of the as-prepared SiC-based NPs, containing carboxylic groups on their surface. The NH₂-graf-ted SiC-NPs(+) are best delivered into the cell nuclei by anionic SAP-E. We anticipate that similar studies will find optimal carrier cell-penetrating peptides (the "matched pairs") for other known NP types.

Notes and references

‡‡ Elemental composition of the nanoparticles can be described as
C100.0H104.1F19.5O51.0 brutto formula.

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