# Identification of NF-kappa B Modulation Capabilities within Human Intestinal Commensal Bacteria

Omar O. Lakhdari, Julien Tap, Fabienne Beguet-Crespel, Karine K. Le Roux, Tomas T. de Wouters, Antonietta A. Cultrone, Malgorzata M. Nepelska, Fabrice F. Lefevre, Joel J. Dore, Herve H. Blottiere

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► To cite this version:

Omar O. Lakhdari, Julien Tap, Fabienne Beguet-Crespel, Karine K. Le Roux, Tomas T. de Wouters, et al. Identification of NF-kappa B Modulation Capabilities within Human Intestinal Commensal Bacteria. Journal of Biomedicine and Biotechnology, 2011, pp.1 - 9. 10.1155/2011/282356. hal-01000996

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Research Article

# Identification of NF-κB Modulation Capabilities within Human Intestinal Commensal Bacteria

Omar Lakhdari,<sup>1,2,3</sup> Julien Tap,<sup>1,2</sup> Fabienne Béguet-Crespel,<sup>1,2</sup> Karine Le Roux,<sup>1,2</sup>

Tomas de Wouters,<sup>1,2</sup> Antonietta Cultrone,<sup>1,2</sup> Malgorzata Nepelska,<sup>1,2</sup> Fabrice Lefèvre,<sup>3</sup>

Joël Doré,<sup>1,2</sup> and Hervé M. Blottière<sup>1,2</sup>

Correspondence should be addressed to Hervé M. Blottière, herve.blottiere@jouy.inra.fr

Received 30 December 2010; Accepted 27 March 2011

Academic Editor: Eric C. Martens

Copyright © 2011 Omar Lakhdari et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

The intestinal microbiota plays an important role in modulation of mucosal immune responses. To seek interactions between intestinal epithelial cells (IEC) and commensal bacteria, we screened 49 commensal strains for their capacity to modulate NF-κB. We used HT-29/kb-seap-25 and Caco-2/kb-seap-7 intestinal epithelial cells and monocyte-like THP-1 blue reporter cells to measure effects of commensal bacteria on cellular expression of a reporter system for NF-κB. Bacteria conditioned media (CM) were tested alone or together with an activator of NF-κB to explore its inhibitory potentials. CM from 8 or 10 different commensal species activated NF-κB expression on HT-29 and Caco-2 cells, respectively. On THP-1, CM from all but 5 commensal strains stimulated NF-κB. Upon challenge with TNF-α or IL-1β, some CM prevented induced NF-κB activation, whereas others enhanced it. Interestingly, the enhancing effect of some CM was correlated with the presence of butyrate and propionate. Characterization of the effects of the identified bacteria and their implications in human health awaits further investigations.

## 1. Introduction

The adult human gut is populated with a large number of commensal bacteria known to influence many aspects of the host gut physiology, including immunity, development, and homeostasis. There is considerable clinical and experimental evidence showing that commensal gut bacteria contribute to immune homeostasis by altering microbial balance or by interacting with the gut immune system [1]. However, cellular and molecular mechanisms by which individual members of the commensal microbiota contribute to immune homeostasis have not been completely elucidated.

Intestinal epithelial cells (IEC) are the first point of contact for bacteria within the gut lumen, and they interact with the gut immune system. Consequently, IEC have a pivotal function in bacteria-host communication. Bacterial signatures generally activate signaling cascades that can trigger proinflammatory gene transcription through specific

receptors (e.g., Toll-like receptors) expressed on apical and/or basolateral surface of epithelial cells. This mechanism is largely controlled by the transcriptional factor NF-κB [2]. NF-κB is a dimeric DNA binding protein whose major form is represented by the association of p65 and p50 proteins. In steady state, NF-κB is locked in the cytoplasm by an inhibitory protein of the IκB family. Upon receptor activation, IκB is phosphorylated by the IκB kinase complex (IKK) before undergoing degradation by the proteasome. Then, free NF-κB translocate to the nucleus to turn on a large number of genes involved in proinflammatory processes at the site of infection or tissue damage.

Obviously, the intestinal epithelium does not trigger inflammatory responses against commensal bacteria. Interestingly, the mechanisms allowing commensal microorganisms to be tolerated by the intestinal mucosa are far from being completely understood. Numerous studies have suggested an involvement of active processes causing

<footnote n="1"><sup>1</sup>INRA, UMR1319, 78350 Jouy-en-Josas, France</footnote>

<footnote n="2"><sup>2</sup>AgroParisTech, UMR Micalis, 78350 Jouy-en-Josas, France</footnote>

<footnote n="3"><sup>3</sup>LibraGen S.A., 31400 Toulouse, France</footnote>
a functional downregulation of the inflammatory response that is generally obtained by interference with the NF-κB pathway. Indeed, previous reports showed the suppression of inflammatory responses in epithelial cells by commensal bacteria either through direct interaction leading to alteration in Toll-like receptors (TLRs) signaling to NF-κB [3, 4] or through direct inhibition of NF-κB transcriptional activity [5]. In addition, some secreted bacterial factors resulting from commensals or probiotics activity have been found to exert anti-inflammatory effects on IEC [6–10].

Furthermore, recent work has ascribed a critical role for NF-κB signaling in maintenance of homeostatic immuno-inflammatory function in the gut [11]. Indeed epithelial NF-κB preserves integrity of the gut epithelial barrier and coordinates antimicrobial actions of the innate and adaptive immune systems. Accordingly, both deficiency in, and hyper-activation of, this transcription factor are underlying factors in chronic inflammatory bowel diseases [12–16].

Finally, determining the microbial factors that positively or negatively regulate this key pathway is of great clinical and scientific interest. Thus, in the present study, we examined the effect of 49 commensal bacteria on the modulation of NF-κB pathway in human IEC models bearing a stable NF-κB-SEAP reporter system. Conditioned media (CM) from these bacteria were tested either alone or in combination with an activator of NF-κB signaling (TNF-α or IL-1β) to identify its inhibitory and enhancing potentials. To compare response profiles between IEC and immune cells, all CM were also tested on human monocyte cell line THP-1 bearing the same NF-κB reporter system.

## 2. Material and Methods

2.1. *Cell Culture and Reagents.* HT-29 cells were grown in DMEM (Sigma) with 2 mM L-glutamine, 50 IU/mL penicillin, 50 µg/mL streptomycin and 10% heat-inactivated fetal calf serum (FCS-Lonza) in a humidified 5% CO₂ atmosphere at 37°C. Caco-2 were cultured in DMEM (Sigma) supplemented with the same amounts of glutamine and antibiotics, 20% heat-inactivated FCS and 1x nonessential amino acids (Invitrogen). The THP-1 blue CD14+ NF-κB reporter clone was obtained from Invivogen and used according to the manufacturer's instruction.

2.2. *Commensal Strains and Preparation of Conditioned Media.* The 49 commensal strains were grown in anaerobic condition at 37°C using the Hungate culture method [17]. Screened strains and corresponding growth media are listed in Table 1.

At the end of the incubation period, bacterial cultures were centrifuged at 5,000 xg for 10 minutes. Bacteria conditioned media (CM) were then collected and filtered on 0.2 µm PES filters. Noninoculated bacteria culture medium served as control.

2.3. *Analyses of NF-κB Activation—SEAP Reporter Assay.* Construction and validation of the NF-κB reporter clones

HT-29/kb-seap-25 and Caco-2/kb-seap-7 have been described previously [18]. For each experiment, Caco-2/kb-seap-7 and HT-29/kb-seap-25 reporter clones were seeded at 50,000 cells per well, into 96-wells plates and incubated 24 hours. Then, cells were stimulated for 24 hours with 10 µL of each tested bacteria CM, for a final volume of 100 µL per well (i.e., 10% vol/vol), in the presence or absence of TNF-α or IL-1β (10 ng/mL final, for HT-29 and Caco-2, resp.).

THP-1 reporter cells were seeded at 100,000 cells per well, into 96-wells plates and stimulated with 10% (vol/vol) of each tested bacteria CM. Cells were then incubated 24 hours prior to quantification of alkaline phosphatase (SEAP).

SEAP in the supernatant was revealed using the Quanti-Blue reagent (Invivogen) according to the manufacturer's protocol and quantified at 655 nm OD. All measurements were performed using a microplate reader (Infinite 200, Tecan).

2.4. *Statistical Analysis.* Results are expressed as mean ± SD. Data were analyzed using Student's t test.

## 3. Results

3.1. *Effect of Bacteria CM on NF-κB Activation in IEC and Monocyte Models.* Out of 49 bacteria CM, 8 and 10 CM significantly activated the NF-κB reporter system on HT-29 and Caco-2 reporter cells, respectively (Figures 1(a) and 1(b)). In fact, the 2 cell lines were largely unresponsive to the vast majority of the tested bacteria CM. Active bacteria CM identified on both epithelial cells belonged to *Clostridium sardiniensis*, *Selenomonas ruminantium*, *Roseburia hominis*, *Roseburia intestinalis*, *Butyrivibrio fibrisolvens*, *Roseburia faecis*, and *Faecalibacterium prausnitzii*. *Bacteroides uniformis* activated NF-κB only on HT-29/kb-seap-25, while a small, nonsignificant stimulation was observed on Caco-2 (P > .05). *Clostridium paraputrificum* and *Parabacteroides distasonis* induced NF-κB activation specifically in Caco-2/kb-seap-7. Although statistically significant, these observed stimulations were lower than the one observed with IL-1β on Caco-2/kb-seap-7 cells (mean 1.03 ± 0.17) or with TNF-α on HT-29/kb-seap-25 (mean 0.92 ± 0.18).

Conversely, almost all bacteria CM activated NF-κB on THP-1 blue reporter cells except those from *Ruminococcus gnavus*, *Ruminococcus obeum*, and *Ruminococcus lactaris* (Figure 1(c)). Stimulation levels measured in HT-29 and Caco-2 were weak in comparison to those obtained in THP-1. Lipopolysaccharide (LPS) used as control in THP-1 cells showed a lower stimulatory effect than most activating CM (mean 0.38 ± 0.13).

3.2. *Effect of Bacteria CM on Activated NF-κB in IEC.* In order to explore the inhibitory and/or enhancing potentials of commensal bacteria CM on NF-κB activation, cotreatment with the proinflammatory cytokines TNF-α (10 ng/mL) or IL-1β (10 ng/mL) was performed on HT-29 and Caco-2, respectively (Figure 2). The positive control of NF-κB activation was noninoculated bacteria culture medium combined with the stimulatory cytokine. The value
<table><caption>TABLE 1: Bacteria strains, references, and growth media.</caption><thead><tr><th>Designation</th><th>Collection reference</th><th>Medium</th><th>Phylum</th></tr></thead><tbody><tr><td>Atopobium parvulum</td><td>DSM 20649T</td><td>M104</td><td>A</td></tr><tr><td>Bifidobacterium adolescentis</td><td>ATCC 15703</td><td>M58</td><td>A</td></tr><tr><td>Bifidobacterium angulatum</td><td>ATCC 27535-CIP 104167</td><td>M58</td><td>A</td></tr><tr><td>Bifidobacterium animalis ssp. animalis</td><td>DSM 20104</td><td>M58</td><td>A</td></tr><tr><td>Bifidobacterium bifidum</td><td>DSM20082/JCM1254</td><td>M58</td><td>A</td></tr><tr><td>Bifidobacterium breve 1</td><td>DSMZ 20091</td><td>M104</td><td>A</td></tr><tr><td>Bifidobacterium breve 2</td><td>ATCC 15701-89/23</td><td>M104</td><td>A</td></tr><tr><td>Bifidobacterium catenulatum</td><td>ATCC27539-CIP104175</td><td>M58</td><td>A</td></tr><tr><td>Bifidobacterium choerinum</td><td>DSM 20434</td><td>M58</td><td>A</td></tr><tr><td>Bifidobacterium dentium 1</td><td>ATCC 15423</td><td>M104</td><td>A</td></tr><tr><td>Bifidobacterium dentium 2</td><td>ATCC 27534-89/20</td><td>M104</td><td>A</td></tr><tr><td>Bifidobacterium gallicum</td><td>ATCC 49850-CIP 103417</td><td>M58</td><td>A</td></tr><tr><td>Bifidobacterium infantis</td><td>DSM20088/ATCC15697</td><td>M58</td><td>A</td></tr><tr><td>Bifidobacterium longum</td><td>ATCC 15707-NCTC 11818</td><td>M58</td><td>A</td></tr><tr><td>Bifidobacterium pseudocatenulatum</td><td>DSM 20438-ATCC 27919</td><td>M58</td><td>A</td></tr><tr><td>Bifidobacterium ruminantium</td><td>ATCC 49390</td><td>M58</td><td>A</td></tr><tr><td>Collinsella aerofaciens</td><td>ATCC 25986</td><td>BHI**</td><td>A</td></tr><tr><td>Propionibacterium acnes</td><td>B15</td><td>M104</td><td>A</td></tr><tr><td>Bacteroides caecae</td><td>ATCC 43185-CIP 104201T</td><td>BHI</td><td>B</td></tr><tr><td>Bacteroides dorei</td><td>DSM 17855</td><td>M104</td><td>B</td></tr><tr><td>Bacteroides fragilis</td><td>B6-AL 2553</td><td>BHI</td><td>B</td></tr><tr><td>Bacteroides ovatus</td><td>ATCC 8483-CIP103756</td><td>M104</td><td>B</td></tr><tr><td>Bacteroides thetaiotaomicron</td><td>ATCC 29148-VPI5482</td><td>M104</td><td>B</td></tr><tr><td>Bacteroides uniformis</td><td>ATCC 8492</td><td>M104</td><td>B</td></tr><tr><td>Bacteroides vulgatus</td><td>ATCC 8482-CIP 103714</td><td>BHI</td><td>B</td></tr><tr><td>Parabacteroides distasonis</td><td>CIP104284-ATCC8503</td><td>BHI</td><td>B</td></tr><tr><td>Parabacteroides johnsonii</td><td>DSM 18315</td><td>M104</td><td>B</td></tr><tr><td>Prevotella copri</td><td>DSM 18205</td><td>M104</td><td>B</td></tr><tr><td>Blautia/Clostridium coccoides</td><td>ATCC 2936</td><td>BHI</td><td>F</td></tr><tr><td>Blautia producta/Ruminococcus productus</td><td>DSMZ 2950-92/46</td><td>M104</td><td>F</td></tr><tr><td>Blautia/Ruminococcus hansenii</td><td>DSM 20583</td><td>M104</td><td>F</td></tr><tr><td>Butyrivibrio fibrisolvens</td><td>DSM3071</td><td>BHI</td><td>F</td></tr><tr><td>Clostridium leptum</td><td>ATCC29065-DSM753</td><td>BHI</td><td>F</td></tr><tr><td>Clostridium nexile 96/2</td><td>ATCC 27757-DSM 1787</td><td>BHI</td><td>F</td></tr><tr><td>Clostridium paraputrificum</td><td>G12PR-X73445</td><td>BHI</td><td>F</td></tr><tr><td>Clostridium sardiniensis</td><td>I11PR</td><td>M78</td><td>F</td></tr><tr><td>Clostridium sordellii</td><td>ATCC 9714</td><td>BHI</td><td>F</td></tr><tr><td>Clostridium sporosphaeroides</td><td>ATCC 25781</td><td>M78</td><td>F</td></tr><tr><td>Dorea/Eubacterium formicigenerans</td><td>ATCC 27755</td><td>L-DON*</td><td>F</td></tr><tr><td>Eubacterium rectale</td><td>ATCC 33656-CIP105953</td><td>BHI</td><td>F</td></tr><tr><td>Faecalibacterium prausnitzii</td><td>L2-6 (Duncan)</td><td>BHI</td><td>F</td></tr><tr><td>Roseburia faecis</td><td>DSM 16840</td><td>BHI</td><td>F</td></tr><tr><td>Roseburia hominis</td><td>DSM 16839</td><td>M58</td><td>F</td></tr><tr><td>Roseburia intestinalis</td><td>DSM 14610</td><td>M58</td><td>F</td></tr><tr><td>Ruminococcus gnavus</td><td>FRE1</td><td>L-DON</td><td>F</td></tr><tr><td>Ruminococcus lactaris</td><td>ATCC 29176</td><td>L-DON</td><td>F</td></tr><tr><td>Ruminococcus obeum</td><td>ATCC 29174</td><td>L-DON</td><td>F</td></tr><tr><td>Ruminococcus torques</td><td>ATCC 27756</td><td>M104</td><td>F</td></tr><tr><td>Selenomonas ruminantium</td><td>ATCC19205-DSM2872</td><td>L-DON</td><td>F</td></tr></tbody></table>

<footnote>* Yeast extract-CaCl2—sodium thioglycholate—pyruvic acid.</footnote>

<footnote>** Brain heart infusion + yeast extract and hemine.</footnote>

<footnote>A: Actinobacteria; B: Bacteroidetes; F: Firmicutes.</footnote>
<figure><img src="image_3.png" /><figcaption>FIGURE 1: Comparison of the stimulatory effect of 49 commensal bacteria conditioned media on HT-29/kb-seap-25, Caco-2/kb-seap-7, and THP-1-blue. NF-κB activity is expressed as OD 655 nm. Data are presented as mean ±/− SD of 3 independent experiments performed in HT-29 (a) and Caco-2 (b) and of 2 independent experiments performed in THP-1 (c). Control is noninoculated bacteria medium and its activity was normalized to 0 (represented by the X-axis). \*P < .05 compared to control. For THP-1 only: ns: not significant. All other values are significantly different from control (P < .05).</figcaption></figure>
<table><thead><tr><th>Strain</th><th>HT-29</th><th>Caco-2</th></tr></thead><tbody><tr><td>A topobium parvulum</td><td>+5.8 (0.374)</td><td>-6.1 (0.233)</td></tr><tr><td>Bifidobacterium adolescentis</td><td>+27.4 (0.067)</td><td>-39.3 (0.048)</td></tr><tr><td>Bifidobacterium angulatum</td><td>+23.9 (0.095)</td><td>-41 (0.046)</td></tr><tr><td>Bifidobacterium animalis</td><td>+27.5 (0.056)</td><td>-23.5 (0.041)</td></tr><tr><td>Bifidobacterium bifidum</td><td>+28 (0.063)</td><td>-32.6 (0.06)</td></tr><tr><td>Bifidobacterium breve 1</td><td>+39.5 (0.035)</td><td>+0.8 (0.464)</td></tr><tr><td>Bifidobacterium breve 2</td><td>+4.7 (0.389)</td><td>-9.3 (0.151)</td></tr><tr><td>Bifidobacterium catenulatum</td><td>+37.3 (0.03)</td><td>-20.7 (0.107)</td></tr><tr><td>Bifidobacterium choerinum</td><td>+40.9 (0.022)</td><td>-20 (0.179)</td></tr><tr><td>Bifidobacterium dentium 1</td><td>+18.1 (0.144)</td><td>-13.1 (0.1)</td></tr><tr><td>Bifidobacterium dentium 2</td><td>+15 (0.212)</td><td>-21 (0.046)</td></tr><tr><td>Bifidobacterium gallicum</td><td>+37 (0.034)</td><td>-8.8 (0.114)</td></tr><tr><td>Bifidobacterium infantis</td><td>+24.9 (0.081)</td><td>-21.5 (0.038)</td></tr><tr><td>Bifidobacterium longum</td><td>+25.3 (0.067)</td><td>-28.8 (0.044)</td></tr><tr><td>Bifidobacterium pseudocatenulatum</td><td>+16.4 (0.203)</td><td>-42 (0.081)</td></tr><tr><td>Bifidobacterium ruminantium</td><td>+21.1 (0.093)</td><td>-24.9 (0.073)</td></tr><tr><td>Collinsella aerofaciens</td><td>-14.1 (0.081)</td><td>-17.1 (0.027)</td></tr><tr><td>Propionibacterium acnes</td><td>+23.7 (0.104)</td><td>-0.8 (0.461)</td></tr><tr><td>Bacteroides caecae</td><td>+11 (0.152)</td><td>-12.6 (0.054)</td></tr><tr><td>Bacteroides dorei</td><td>+8.3 (0.306)</td><td>-12.1 (0.106)</td></tr><tr><td>Bacteroides fragilis</td><td>+18.6 (0.043)</td><td>+3.4 (0.322)</td></tr><tr><td>Bacteroides ovatus</td><td>+10.8 (0.247)</td><td>-9.6 (0.167)</td></tr><tr><td>Bacteroides thetaiotaomicron</td><td>+11 (0.24)</td><td>-2.5 (0.275)</td></tr><tr><td>Bacteroides uniformis</td><td>+110.2 (0.002)</td><td>+43.5 (0.016)</td></tr><tr><td>Bacteroides vulgatus</td><td>+14.4 (0.120)</td><td>-16.4 (0.029)</td></tr><tr><td>Parabacteroides distasonis</td><td>+22.4 (0.065)</td><td>+0.6 (0.467)</td></tr><tr><td>Parabacteroides johnsonii</td><td>+16.8 (0.173)</td><td>-5.5 (0.290)</td></tr><tr><td>Prevotella copri</td><td>+7.2 (0.326)</td><td>-15.8 (0.066)</td></tr><tr><td>Blautia coccoides</td><td>+11.6 (0.088)</td><td>-13.3 (0.049)</td></tr><tr><td>Blautia hansenii</td><td>+7.2 (0.322)</td><td>-11.5 (0.149)</td></tr><tr><td>Blautia producta</td><td>+14.2 (0.210)</td><td>-18.2 (0.057)</td></tr><tr><td>Butyrivibrio fibrisolvens</td><td>+3.5 (0.331)</td><td>-13.5 (0.052)</td></tr><tr><td>Clostridium leptum</td><td>-10.4 (0.137)</td><td>-16.9 (0.03)</td></tr><tr><td>Clostridium nexile 96/2</td><td>-0.4 (0.475)</td><td>-20 (0.017)</td></tr><tr><td>Clostridium paraputrificum</td><td>+8.6 (0.139)</td><td>+17.8 (0.055)</td></tr><tr><td>Clostridium sardiniensis</td><td>+45.3 (0.02)</td><td>+51.6 (0.001)</td></tr><tr><td>Clostridium sordellii</td><td>-20.9 (0.035)</td><td>-8.4 (0.118)</td></tr><tr><td>Clostridium sporosphaeroides</td><td>+42.9 (0.008)</td><td>+45.8 (0.0004)</td></tr><tr><td>Dorea formicigenerans</td><td>+14.1 (0.091)</td><td>-1 (0.337)</td></tr><tr><td>Eubacterium rectale</td><td>-3.5 (0.333)</td><td>-14.8 (0.042)</td></tr><tr><td>Faecalibacterium prausnitzii</td><td>+33.5 (0.012)</td><td>+10 (0.104)</td></tr><tr><td>Roseburia faecis</td><td>+42.2 (0.003)</td><td>+34.5 (0.025)</td></tr><tr><td>Roseburia hominis</td><td>+51 (0.016)</td><td>+37.9 (0.002)</td></tr><tr><td>Roseburia intestinalis</td><td>+61.2 (0.009)</td><td>+45.6 (0.009)</td></tr><tr><td>Ruminococcus gnavus</td><td>-1.7 (0.435)</td><td>-5.2 (0.120)</td></tr><tr><td>Ruminococcus lactaris</td><td>+5.1 (0.250)</td><td>+0.6 (0.382)</td></tr><tr><td>Ruminococcus obeum</td><td>+3.3 (0.342)</td><td>-1.5 (0.275)</td></tr><tr><td>Ruminococcus torques</td><td>-4.1 (0.411)</td><td>-11.4 (0.116)</td></tr><tr><td>Selenomonas ruminantium</td><td>+21.6 (0.02)</td><td>-6.3 (0.194)</td></tr></tbody></table>

(a)

<table><thead><tr><th>Color code</th><th>No significant effect</th></tr></thead><tbody><tr><td></td><td>Inhibitory with P value < .1</td></tr><tr><td></td><td>Inhibitory with P value < .05</td></tr><tr><td></td><td>Stimulatory with P value < .1</td></tr><tr><td></td><td>Stimulatory with P value < .05</td></tr></tbody></table>

(b)

FIGURE 2: (a) Effect of bacteria CM on activated NF-κB in HT-29 and Caco-2 reporter cells. NF-κB activity is expressed as a relative percentage compared to the positive control (normalized to 0). Positive controls are cells treated with noninoculated bacteria culture medium and the stimulatory cytokine. The corresponding *P* value is indicated in parenthesis and inhibitory and stimulatory strains are identified following the color code shown in (b).

of NF-κB activity measured for the positive control was normalized to 0. The values obtained for each strain were expressed as percentage of activation relative to that of the positive control. For example, *Selenomonas ruminantium*

CM combined with TNF-α induced NF-κB activity 21.6% higher than the one obtained with the positive control (i.e., noninoculated bacteria medium combined with TNF-α).
<figure><img src="image_4.png" /><figcaption>FIGURE 3: Effect of SCFA and organic acids on NF-κB activation. HT-29 cells were treated with acetic, butyric, propionic, lactic, succinic, and formic acids either alone or in combination with TNF-α (10 ng/mL) for 24 hours. Results are expressed as OD 655 nm. Data is representative of 1 experiment out of 3 performed. \*P < .05 compared to TNF control.</figcaption></figure>

A different behavior of the 2 epithelial models in response to the bacteria CM could also be observed (Figure 2). Indeed on HT-29, CM overall enhanced NF-κB activity more than in Caco-2. Interestingly, a large majority of Bifidobacteria CM were stimulatory on HT-29 while inhibitory on Caco-2.

Some bacteria CM had a similar effect on the 2 reporter cells (Figure 2). Induced NF-κB activation was restrained in the 2 cell lines only by *Colinsella aerofasciens*, with inhibition rates of 14.1% (*P* = .081) and 17.1% (*P* = .027) for HT-29 and Caco-2, respectively. Furthermore, the 3 species of *Roseburia* as well as *Clostridium sardiniensis*, *Clostridium sporosphareoides*, and *Bacteroides uniformis* enhanced NF-κB activation significantly in both cell lines.

We also observed that some CM had effects only on one reporter cell line such as CM from *Selenomonas ruiminantium*, *Faecalibacterium prauznitzii*, *Bacteroides fragilis*, *Parabacteroides distasonis*, and *Bifidobacterium breve* 1. These CM increased NF-κB activity in HT-29/kb-seap-25

but exhibited nonsignificant effects on Caco-2/kb-seap-7. Similarly, *Eubacterium rectale*, *Clostridium nhexile 96/2*, *Clostridium leptum*, *Blautia coccoides*, and *Bacteroides vulgatus* exerted significant inhibitory effects only on Caco-2.

### 3.3. Effect of Short Chain Fatty and Organic Acids on NF-κB Activation in IEC
Commensal bacteria are known to produce a panel of acids during their metabolic activity, especially short chain fatty acid (SCFA), which could interfere with NF-κB response.

Therefore, we evaluated the effects of acetic, butyric, propionic, lactic, succinic, and formic acids on NF-κB in IEC, either alone or on cytokine-activated cells (Figure 3). The results presented were obtained on HT-29 although similar observations were performed on Caco-2 (data not shown).

None of the acids had an effect on HT-29 or Caco-2 when used alone. However, butyrate and propionate produced a dose-dependent hyperactivation of NF-κB on TNF-α activated cell. The other acids induced a small but significant stimulatory effect only at the highest concentration (6–8 mM final) and a very small but yet significant inhibitory effect of NF-κB at the lowest concentrations. Since butyrate and propionate are likely to act on activated NF-κB signaling, we quantified SCFA in the CM by HPLC and examined their associations with NF-κB activity. We found that out of 49 bacteria CM, 19 contained butyrate, propionate, or both (Figure 4).

Figure 4 represents a plot of the amount of butyrate (Figure 4(a)) or propionate (Figure 4(b)) present in each bacteria CM (X-axis) with the NF-κB activity measured in HT-29 in response to cotreatment with bacteria CM and TNF-α (see Figure 2). A Spearman correlation test was performed by taking into account butyrate and propionate concentrations greater than 1 mM. A significant positive correlation (r = 0.76, *P* = .036) was observed between butyrate concentration and NF-κB activity, suggesting that the butyrate-producing strains might have exerted their stimulatory effect through the butyrate released during growth. Similarly, most of the propionate-producing bacteria were also stimulatory on HT-29, but the correlation between propionate concentration and NF-κB activity was not significant (r = 0.49, *P* = .075). However, the correlation became highly significant when only strains from the Bacteroidetes phylum (r = 0.81, *P* = .005) were included in the analyses. This suggests that the effect of the other propionate-producing strains might be due to other active metabolites that are different from propionate.

## 4. Discussion

In this study, we aimed at identifying commensal strains deemed capable of regulating eukaryotic cell signaling focusing on the NF-κB signaling pathway, which is largely involved in immune and inflammatory responses.

In the IEC models HT-29 and Caco-2, the majority of bacteria CM had no effect on NF-κB, contrasting with the results obtained with the monocyte cell line THP-1. This observation may be explained by the differences
<figure><img src="image_5.png" /></figure>

FIGURE 4: Correlation between butyrate or propionate concentration of the bacteria CM and NF-κB activity. NF-κB activity measured in HT-29 in response to cotreatment with bacteria CM and TNF-α (10 ng/mL) based on the amount of butyrate (a) and propionate (b) present in each bacteria CM. NF-κB activity is expressed as a relative percentage (values from Figure 2). Butyrate and propionate amounts were determined by HPLC and expressed in mM. A Spearman correlation test was performed: Butyrate *versus* NF-κB activity: *r* = 0.76 (*P* = .036). Propionate *versus* NF-κB activity: *r* = 0.49 (*P* = .075). Propionate (Bacteroidetes only) *versus* NF-κB activity: *r* = 0.81 (*P* = .005).

in the expression of receptors specialized in recognition
of microbial structures, especially TLRs. Indeed, THP-1
reporter clone as well as the parental cell line express a
complete range of TLRs and all five surface TLRs (TLR1/2,
TLR2, TLR6/2, TLR4, and TLR5) are present and functional
[18–20], resulting in increased sensitivity to a wide range
of microbe-associated molecular patterns. Conversely, HT-
29 and Caco-2 do not express all TLRs. Parental HT-29
cell line as well as the reporter clone HT-29/kb-seap-25
mainly express a functional TLR5, which is responsible for
detection of flagellin [21]. Caco-2 parental cell line was also
described to be responsive to TLR5 stimulation [22, 23],
but the reporter clone Caco-2/kb-seap-7 that was obtained
and used throughout our study is poorly sensitive [18].
In addition, unresponsiveness toward stimulation of other
TLRs such as TLR2 and TLR4 was reported for parental
intestinal epithelial cell lines [24–26] and was also observed
with our IEC reporter clones [18].

Furthermore, the fact that HT-29/kb-seap-25 is sensitive
to flagellin explains the basal stimulatory effect displayed by
the CM from flagellated commensal strains such as Roseburia
spp., Selenomonas ruminantium and Butyrivibrio fibrisolvens
[27–29]. These flagellated commensals also stimulated NF-
κB in Caco-2/kb-seap-7, but these cells are less sensitive

to flagellin than HT-29 which explains the weak response
obtained. Stimulation by *Bacteroides uniformis* and *Faecal-
*ibacterium prausnitzii* CM on both cell lines was not likely
due to a putative flagellin expression since these bacteria are
not motile [30, 31], which is not the case with *Clostridium*
*sardiniensis* [32].

*Clostridium paraputrificum*, *Parabacteroides distasonis*, and *Bifidobacterium breve* 1 CM stimulated Caco-2 but not HT-29, supporting the assertion that the effect is independent of TLR5 recognition. Since Caco-2 reporter cells are not sensitive to TLRs stimulation [18], the specific stimulatory effect of these 3 bacteria CM need further evaluation. In addition, the lack of response of THP-1 toward CM from 3 different strains of *Ruminococcus* is an intriguing observation that also deserves attention.

The 2 reporter cells used in this study were stimulated by
the commensal bacteria CM combined with a known NF-κB
activator. This challenge was performed in order to identify
both the stimulatory as well as the inhibitory contributors of
NF-κB signaling activity. The 2 proinflammatory cytokines
TNF-α and IL-1β were used to activate NF-κB on HT-29 and
Caco-2, respectively, [18]. Under these conditions, some CM
remarkably dampened NF-κB activation in these cells with
the inhibition occurring mainly in IL-1β-activated Caco-2.

Several *Bifidobacterium* strains were described for their
capacity to dampen NF-κB activation *in vitro* in different cell
models, including Caco-2 and HT-29 [33, 34]. Consistent
with this, few *Bifidobacterium* CM reduced NF-κB activation
on Caco-2, but were ineffective on HT-29. In contrast, in
the present study, CM from *Bifidobacterium* strains enhanced
NF-κB activation by TNF on HT-29. However, in our study,
we measured the transcriptional activity of NF-κB instead of
the product of one gene controlled by NF-κB. It is well known
that NF-κB controls the activation of several genes not
only involved in inflammatory processes, but also in tissue
protection and homeostasis. For example, production of
human β-defensin in IEC, which is NF-κB-dependent [35],
is stimulated by probiotics, including the *Bifidobacterium*-
containing mixture VSL#3 [36]. Obviously, this dual effect of
*Bifidobacterium* strains on NF-κB signaling requires further
examination.

Butyrate (or butyric acid) and propionate, 2 products of
bacterial fermentation, enhanced NF-κB activation induced
by TNF-α or IL-1β in our reporter cells. As such, butyrate-
producing bacteria stimulated NF-κB activity and a strong
correlation has been found between bacteria CM butyrate
concentration and NF-κB activity.

However some propionate-producing bacteria, such as *Eubacterium rectale* and *Clostridium leptum* did not enhance NF-κB, suggesting the existence of other metabolites that may counter the stimulatory effect of propionate on activated epithelial cells.

It is noteworthy that although butyrate is classically
known for preventing NF-κB activation in IEC [37–39],
some recent studies suggest that butyrate also promotes
NF-κB transcriptional activity in IEC [40, 41]. In addition,
butyrate has been shown to promote human β-defensin
expression, whose gene transcription is controlled by NF-κB
[42].
**5. Conclusion**

The mechanisms underlying the inhibitory and stimulatory effects of NF-κB signaling in monocyte and IEC models by nonbutyrate-producing and nonflagellated bacteria strains remain to be explored. The cell-based screening method employed in the present study provides a rapid identification of potentially interesting commensal species; however, their effects require further confirmation and characterization using other techniques of NF-κB detection. Moreover, the potential implication of these commensal bacteria and their host cells regulating properties in human health and disease may need to be evaluated.

**Acknowledgments**

The authors thank Chantal Bridonneau for providing the bacteria strains and Pascal Courtin for HPLC analysis. They are very grateful to Mihai Covasa for revising the paper for English.

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