**POSTER SESSIONS 1 AND 2 – Monday and Tuesday –** all odd number posters will be on display.

**POSTER SESSIONS 3 AND 4 – Wednesday and Thursday –** all even number posters will be on display.

*AWARD WINNERS

TECHNOLOGY

P-568

Combination of UHPLC coupled to Q-TOF and QQQ provides substantial benefits for metabolic pathway profiling

PRESENTING AUTHOR: Katrin Freiburghaus, University Institute of Clinical Chemistry, Switzerland

CO-AUTHORS: Carlo Largiadèr, Christoph Stettler, Martin G. Fiedler, Lia Bally, Cédric Bovet

Non-targeted metabolomics by UHPLC coupled to high-resolution mass spectrometry (HRMS) is often applied for identification of regulated pathways in biological samples. Although this approach facilitates the discovery of new metabolites, it suffers from detector saturation and a complex integration pipeline. The simple data integration and scheduling acquisition has fostered the use of triple quadrupoles (QQQ) for profiling hundreds of metabolites in a single run. Here, we demonstrate the benefits of a joint use of non-targeted HRMS and targeted QQQ metabolic profiling to characterize exercise-induced metabolic changes in serum of type-1-diabetes (T1D) patients. In a crossover study, 12 male adults with T1D underwent 90 min of intermittent high-intensity or iso-energetic continuous moderate-intensity exercise. Metabolic profiling of serum collected before, during and after exercise was performed by UHPLC-HRMS (Q-TOF) and QQQ. The QQQ method covered 114 metabolites of which 97 were detected in the samples. Amongst the 83 commonly detected metabolites, intra-assay precision was increased up to 2-fold using QQQ compared to HRMS. Up to 61% of the detected metabolites had an excellent linearity over three orders of magnitude (r2 >0.99) with the QQQ, while only 29% with the HRMS. Selected pathway coverage was improved up to 3-fold by the QQQ: 62% metabolite coverage of purine metabolism and 33% coverage of citric acid cycle were achieved. Our screening strategy enhanced metabolic profiling in serum of T1D patients by combining the benefits of unbiased full-scan HRMS acquisition with extended insights into specific pathways by QQQ analysis.

P-569

Micro-LC-MS versus UHPLC-MS analysis of endocannabinoids in cerebrospinal fluid: the potential role of the endocannabinoid system in migraine pathology

PRESENTING AUTHOR: Xinyu Di, LACDR, Netherlands

CO-AUTHORS: Faisa Guled, Elke Krekels, Isabelle Kohler, Thomas Hankemeier.

Experimental and clinical data have suggested a link between migraine pathology and the endocannabinoid system (ECS). In this study, we aim to compare endocannabinoid levels in cerebrospinal fluid (CSF) and plasma samples in migraine patients (N = 195) versus healthy controls (N = 94). The problem here is, the concentration of several endocannabinoids in CSF is very low (sub-ng/mL range), lower than the limits of detection (LODs) offered by conventional UHPLC-MS techniques, which highlights the need for highly sensitive detection methods. To achieve this goal, a micro-LC-MS method has been developed and optimized for the targeted analysis of 17 endocannabinoids. The separation was performed using a micro Acquity HSS T3 column (0.3 × 100 mm, 1.8 µm) at a flow rate of 10 µL/min. An injection volume of 4uL is applied by utilizing the enrichment function of the column. Electrospray ionization and selective Multiple Reaction Mode was used for data acquisition. The lower limits of quantification (LLOQs) obtained with the developed micro-LC-MS method were 5 to 10 times lower than the LLOQs obtained with state-of-the-art UHPLC-MS method using the same mass analyzer. The developed method enabled the detection of the 17 targeted endocannabinoids in human CSF. This method will be applied to the analysis of migraine patient samples.

P-570

Balancing quantitative and qualitative LC-HRMS for simultaneous targeted and non-targeted metabolomics

PRESENTING AUTHOR: Anne-Charlotte Dubbelman, Leiden University, Netherlands

CO-AUTHORS: Filip Cuyckens, Lieve Dillen, Rob J. Vreeken, Thomas Hankemeier

A typical trend in metabolomics is to do more for less. In mass spectrometry (MS), this induces the shift from using triple quadrupole MS for targeted and more quantitative metabolomics versus high-resolution MS (HRMS) for non-targeted metabolomics, towards using HRMS for both at the same time (Quan/Qual). Although theoretically attractive, practically, optimizing HRMS parameter settings for qualitative analysis can compromise the quantitative analysis and vice versa. Therefore, we investigated the often underestimated effect of selected HRMS parameter settings on the quantitative and qualitative performance. Human plasma was used for all evaluations and a wide variety of drugs was spiked to optimize for (pharmaco-)metabolomics. On ultra-high performance liquid chromatography (UHPLC) coupled to quadrupole Time-of-Flight MS systems, we varied and evaluated the effects of varying the scan protocol, mass resolution, scan time and smoothing on the measurements. It was found that especially for high-throughput UHPLC, the scan time (cycle time) is critical in Quan/Qual analysis. A longer cycle time allows more elaborated qualitative scan protocols, e.g. including fragmentation without or after ion mobility separation, apart from just ToF-MS. However, a too long scan time (here already observed from 200 ms) resulted in the co-detection of chromatographically separated isomers. Using a Synapt G2S MS, the amount of qualitative information correlated negatively with the quantitative performance (precision, accuracy, linear dynamic range, sensitivity). To conclude, optimizing HRMS settings for simultaneous targeted and nontargeted metabolomics requires balancing parameter settings for quantitative and qualitative performance. This study resulted in recommendations to help future Quan/Qual LC-HRMS method development.
<figure><img src="image_91.png" /></figure>

**POSTER SESSIONS 1 AND 2 – Monday and Tuesday –** all odd number posters will be on display.

**POSTER SESSIONS 3 AND 4 – Wednesday and Thursday –** all even number posters will be on display.

*AWARD WINNERS

TECHNOLOGY

P-571

A REAL TIME METABOLOMICS PROFILING APPROACH USING RAPID EVAPORATIVE IONIZATION MASS SPECTROMETRY (REIMS) TO CLASSIFY MEAT SAMPLES

PRESENTING AUTHOR: Yann Guitton, Laberca, France

CO-AUTHORS: G. DERVILLY-PINEL, S. STEAD, B. LE BIZEC

In recent years the development of Ambient Mass Spectrometry (AMS) techniques has boomed. AMS technics are convenient when it comes to high-throughput and complex samples analysis, as it allows the analyst to bypass long sample preparation steps and gets "instantaneous" results. Among them, Rapid Evaporative Ionization Mass Spectrometry (REIMS) is promising for classification of tissue samples. In short, samples are simply "burned" and the created aerosol is ionised and directly analysed in full scan mode. On tissue samples, the REIMS generated mass spectrometric profiles are dominated by lipids. That specificity is used to discriminate samples by their lipidic fingerprint. The combination of REIMS with untargeted metabolomics workflow was investigated to identify carcasses from pig treated animals on the basis of a modification of indirect metabolites profile due to the use of Ractopamine, a β-agonist substance that may be used in some parts of the world as growth promoter in livestock, although forbidden in a number of countries. The strategy was found successful when tested on different muscle types (loin, shoulder and ham). Multi-variate statistical software package LiveIDTM (Waters) was used as a model builder and direct recognition tool. Classification performances were 0% false negative and 10% false positive, which fully answers requirements of a screening strategy. REIMS implemented in an untargeted-metabolomics workflow can be considered as a high-throughput and powerful strategy for real-time meat classification in relation to Ractopamine treatment in pig. [1] Guitton et al DOI: 10.1080/19440049.2017.1421778 *: Corresponding author: e-mail: laberca@oniris-nantes.fr

P-572

Standardization and Quantitative Analysis in Targeted Metabolomics

PRESENTING AUTHOR: Therese Koal, Biocrates Life Sciences AG, Austria

CO-AUTHORS: Hai Pham Tuan, Doreen Kirchberg, Barbara Wolf

Quantitative metabolite analysis of related biochemical pathways is of high interest for a better understanding of health and disease. Mass spectrometry is a key analytical technique presently used in metabolomics. Reliable analytical results and improved inter-laboratory comparability, automation, and standardization of a metabolomics workflow is of utmost importance to deliver translational data for next-gen metabolomics. Here, we present the standardized, quantitative AbsoluteIDQ® p180 kit-assay as a solution for metabolic phenotyping on an Agilent triple quadrupole LC/MS system allowing the multiplexed and targeted analysis of up to 188 endogenous metabolites and lipids from six different key metabolite classes (21 amino acids, 21 biogenic amines, 40 acylcarnitines, hexoses, 15 sphingolipids, and 90 glycerophospholipids). The AbsoluteIDQ® p180 kit is applicable to a broad range of biological matrices (blood, tissue, cell culture etc.) and species and requires only 10 µL of sample volume. An easy-to-use and rapid sample preparation protocol with a specially designed 96-well filter plate allows high-throughput analysis. The LC-MS/MS instrument consists of the 1290 Infinity II LC system coupled to a 6470 triple quadrupole mass spectrometer. Automated data analysis of >22K MRM chromatograms from >230 metabolites and internal standards analyzed in 96 tests (blanks, QC, standards, and samples) was performed using Biocrates' MetIDQ™ software, which automatically controls the entire workflow, from sample registration to data processing and result reporting. Analytical performance, including lower and upper limits of quantitation, intra- and inter-batch accuracy, and precision will be presented. In addition, a comparative study of common biofluids relevant for metabolomics will be shown.

P-573

MxP® Quant 500 Kit – Novel Standardized Metabolomics/Lipidomics Analysis Tool for Comprehensive Targeted Profiling

PRESENTING AUTHOR: Ulf Sommer, Biocrates Life Sciences AG, Austria

CO-AUTHORS: Hai Pham Tuan, Svenja Heischmann, Doreen Kirchberg, Xenia Iwanowa, Radu Talmazan, Barbara Wolf, Martin Buratti, Rosa Argamasilla Martinez, Cornelia Röhring, Therese Koal

For comprehensive metabolomics/lipidomics analysis, analytical reliability, inter-laboratory comparability, automation, and standardization are of utmost importance. Here, we present the newly developed quantitative MxP® Quant 500 kit-based assay for multiplexed MS/MS analysis of 630 metabolites/ lipids from 26 analyte classes in only 10 µL sample volume. The assay allows standardized analysis in a variety of biological sample matrices (e.g. blood, feces, tissue) and species, including the microbiome. The ready-to-use assay combines UHPLC- and FIA-MS/MS into a single workflow. Automated data analysis of analytes and internal standards analyzed in 96-well format (blanks, QCs, standards, and samples) was performed with Biocrates' MetIDQ™ software, which controls the entire workflow from sample registration to data processing and reporting. For beta-testing 14 samples (NIST SRM 1950, 6 human plasma samples, 2 human serum samples, lipemic human plasma, mouse plasma, rat plasma, mouse liver, and human feces) were evaluated. The assay was successfully validated following EMA/FDA guidelines. Absolute quantitation in biological samples was achieved using a seven-point calibration or a one-point calibration with stable-isotope labeled internal standards. The analytical accuracy and precision of the assay were safeguarded by three levels of QC samples. We will present the analytical workflow and performance parameters, including intra/inter-batch accuracies and precision, and accuracy data of reference materials. MetIDQ™ software enabled data processing including quality assessment and the combination of all results. Beta-test data across 8 independent data sets across the 14 samples will be presented showing excellent accuracies and precision results, the basis for longitudinal robustness and inter-laboratory comparability.
**POSTER SESSIONS 1 AND 2 – Monday and Tuesday –** all odd number posters will be on display.

**POSTER SESSIONS 3 AND 4 – Wednesday and Thursday –** all even number posters will be on display.

*AWARD WINNERS

TECHNOLOGY

P-574 A New HILIC LC/Q-TOF Method for Metabolomics Analysis with Important Isomer Separation and Broad Coverage of Metabolite Classes

PRESENTING AUTHOR: Yuqin Dai, Agilent Technologies, United States

CO-AUTHORS: Jordy J. Hsiao

A robust and high performance HILIC LC/Q-TOF method was developed for the detection of broad classes of endogenous metabolites. The chromatographic gradient, mobile phase pH, buffer concentration, and column temperature were optimized to achieve a baseline separation for 10 pairs of biologically important isomers. Moreover, the effect of sample salt concentration on the reproducibility of retention time (RT) and signal response was investigated. The preliminary results showed that RT and signal response reproducibility was excellent in the presence of 80 mM of NaCl and 160 mM urea. Phosphorylated metabolites such as sugar phosphates and nucleotides are often challenging to analyze due to their interactions with stainless steel in the system. By incorporating medronic acid to deactivate the steel surfaces, CV values were within 0.2% and 10% for RT and signal response respectively. The effect of source conditions on MS response was also evaluated. The results showed that gas temperature and nozzle voltage play a key role in altering the signal response. Low temperature favored labile organic acids, while high temperature enhanced phosphorylated metabolites. By using optimal source conditions, this method enabled the detection of broad classes of metabolites, including amino acids, vitamins, polyamines, carboxylic acids, sugars and sugar phosphates, nucleotides, cofactors and coenzyme A (CoA) derivatives. This new HILIC LC/Q-TOF method clearly demonstrated a superior analytical performance in terms of the chromatographic separation, analytical reproducibility, and global coverage of metabolites. Implementation of this method for the analysis of real-world biological samples is being conducted.

P-575 High-throughput lipidomics and metabolomics analysis directly on genetically modified CHO cells using Laser-Ablation Rapid Evaporative Mass Spectrometry (LA-REIMS)

PRESENTING AUTHOR: Stefania Maneta-Stavrakaki, Department of Surgery and Cancer, Imperial College London, United Kingdom

CO-AUTHORS: Julia Abda, Alvaro Perdones-Montero, Simon J. Cameron, Yuen-Ting Chim, Paloma Diaz-Fernandez, Zoltán Takáts

Introduction - Chinese Hamster Ovary (CHO) cells are used as host cells for the production of recombinant proteins. The analysis of CHO cells is difficult, due to the time-consuming extraction methods, which can create a bias regarding the cells' biological profile. Here, we report a high-throughput lipidomic and metabolomic analysis in ambient conditions, with minimal sample treatment, using Laser-Ablation Rapid Evaporative Ionization Mass Spectrometry (LA-REIMS) that allows the cell analysis in less than 10 seconds per sample. Methods - LA-REIMS was performed on the cell pellets of five CHO-K1 genetically modified cell lines – producing different amounts of the recombinant protein adalimumab – using an OPO (optical parametric oscillator) and a CO2 laser, connected through a REIMS source interface to a QToF mass analyser. The CO2 laser offers automated analysis, since it is implemented on a TECAN platform, which allows the analysis of hundreds of samples per day. Results - LA-REIMS differentiated the five cell lines and provided many m/z values that could be of biological importance regarding the recombinant protein expression and proved to be a suitable technique for the fast analysis of cell pellets in ambient environment with minimal sample pretreatment. The next steps of the current study include MS/MS analysis on the statistically significant m/z values. Conclusion - LA-REIMS was used for high-throughput analysis of CHO cells with minimal pre-treatment. It is a fast method that can provide a biological snapshot of cells in ambient conditions.

P-576 Reference-free metabolite identification by infrared ion spectroscopy

PRESENTING AUTHOR: Kas Houthuijs, Radboud University, Netherlands

CO-AUTHORS: Giel Berden, Udo Engelke, Leo Kluijtmans, David Wishart, Ron Wevers, Karlien Coene, Jos Oomens and Jonathan Martens

A major challenge in metabolomics is identifying the full molecular structure of low-abundance small molecules. Nanomolar concentrations can be detected by combining liquid chromatography and (tandem) mass spectrometry (LC-MS), but structural information is limited to retention times and (fragment) masses, which are often inconclusive. Recently, infrared ion spectroscopy (IRIS) has emerged as a powerful analytical technique to elucidate molecular structure. IRIS combines the sensitivity of LC-MS with the structural information of gas-phase IR spectroscopy and has been successfully applied for the identification of low-abundance biomarkers. However, identification using IRIS is time consuming and relies heavily on reference compounds that are often not readily available. Here we present an automated workflow to assist in the identification of detected features in an LC-MS experiment using IRIS and computational chemistry. We are able to theoretically predict IR spectra for candidate molecules that can subsequently be scored against the experimental IR spectrum of an observed m/z feature. By linking the workflow to the human metabolome database (HMDB), the user is allowed to specify search criteria which are used to find and generate candidate structures. We benchmark the workflow by identifying metabolites using only their chemical formula and experimental IR spectra as input information. Results show that the workflow is successful in scoring the correct metabolite as the best match. Additionally, it assigns high scores to metabolites closely related in structure. The latter shows the specificity of IR and the potential the workflow has in aiding the identification of unknown unknowns.
POSTER SESSIONS 1 AND 2 – Monday and Tuesday – all odd number posters will be on display.

POSTER SESSIONS 3 AND 4 – Wednesday and Thursday – all even number posters will be on display.

*AWARD WINNERS

TECHNOLOGY

P-577

How to automate boring lipidomic extraction

PRESENTING AUTHOR: Justine Bertrand-Michel, MetaToul-Lipidomic, France

CO-AUTHORS: Julia SOULLIER, Aurélie BATUT, Jessica DALLOUX-CHIOCCIOLI, Anthony TOURNADRE, Pauline LE FAOUDER

Lipids are ubiquitous biomolecules essential to all life, found in every cellular type, ranging from the human body and vegetal organisms, down to bacteria. They have many different functions in cell structuration, energy storage and signalling so they are natural biomarkers for different diseases like cancer, cardiovascular disease, neurodegenerative disease, lung disease, their study and quantification are then crucial. Mass spectrometry (MS) coupled with liquid chromatography or gas chromatography is mainly used for global and specific analysis of lipids. But before their analysis, there is an important and time consuming step of sample preparation. Due to their amphiphilic properties, there are usually two types of extractions: liquid-liquid extraction (LLE) and solid phase extraction (SPE). LLE and SPE are two very long protocols which are tedious when there are massive numbers of samples. They can also be the source of many errors, considering the experimenter-dependant possible repeated artefacts. To circumvent this point and increase the analytical service delivery, a TECAN robot has been acquired by MetaToul-Lipidomic facility to automate the sample preparation. Due to the specificity of lipid extraction, the robot needs a lot of optimization. This presentation will show part of adaptation we had to perform to validate fatty acid, neutral lipid and phospholipid profiling. That was related to the precision of solvent sampling which implies new adjustment accuracy for each of them, adding air gap, but also to modify the speed... First lipidomic's results obtained with a complete automated sample preparation will be presented for liver and plasma sample.

P-578

Novel analytical method for global metabolomic analysis by hydrophilic interaction/anion-exchange liquid chromatography tandem mass spectrometry

PRESENTING AUTHOR: Takeshi Bamba, Kyushu University, Japan

CO-AUTHORS: Kohta Nakatani, Yoshihiro Izumi, Masatomo Takahashi, Keita Sakurai, Michio Butsugan, Takeshi Bamba

Essential metabolic pathways to maintain biological activities include glycolysis, tricarboxylic acid cycle, amino acid, and nucleic acid metabolism. All intermediate of these metabolic pathways are polar ionic compounds. A profiling for these hydrophilic metabolites is the key to understand metabolic regulation. However, it is difficult to comprehensively measure them with a single analytical run by conventional analytical method such as reversed phase liquid chromatography (RPLC), hydrophilic interaction liquid chromatography (HILIC), or ion chromatography (IC), coupled with mass spectrometry. In this study, we developed a novel analytical method for global metabolic analysis using hydrophilic interaction/anion exchange liquid chromatography tandem mass spectrometry (HILIC/AEX/MS/MS). To construct a practical analytical system for global metabolic analysis, we developed a novel amino-polymer packed column, which was composed of polymer particles with amines as functional groups. The effect of the LC conditions on retention behaver and peak shapes of 45 representative hydrophilic metabolites was evaluated. As a result, the optimized mobile phase was 40 mM ammonium bicarbonate at pH 9.8 (A) and acetonitrile (B). After cationic and zwitterionic metabolites were eluted under HILIC condition (t = 1-15 min, 95-40%B), almost of all anionic metabolites were eluted under AEX condition (t = 15-16 min, 40-0%B; t = 16-26 min, 0%B). Thus, we named this chromatographic technique "HILIC/AEX". With the optimal method conditions established, we successfully identified approximately 200 hydrophilic metabolites from HeLa cell extracts. Taking all of the present results into account, our advanced analytical system will be useful tool for in-depth studies on global metabolism.

P-579

METABOLOMICS / LIPIDOMICS OF DRIED BLOOD SPOTS USING A NOVEL BLOOD MICROSAAMPLING DEVICE

PRESENTING AUTHOR: Konstantinos Kouremenos, Trajan Scientific and Medical, Australia

CO-AUTHORS: Konstantinos Kouremenos, Christopher Bowen, David de Souza, Kannan Ragunathan, Jason Hon, Dedreia Tull, Florian Lapierre, Anne Collins, Andrew Gooley

Dried blood spots (DBS) offer many advantages over plasma, serum, and whole blood sampling, including being minimally invasive and easy to collect and store. DBS have been extended to applications such as therapeutic drug monitoring, pharmacokinetics, genomics, proteomics, lipidomics and metabolomics. A novel blood collection and storage device collects an accurate blood volume, controlled through the use of precision glass capillaries of a pre-determined volume (2.74 uL per capillary). The device provides 4 x 2.74uL replicates from the same blood source, which are then transferred to a blood storage substrate all encapsulated in a controlled desiccated environment to maintain sample integrity. Hence, the utility of the device is ideal for longitudinal metabolomic/lipidomic profiling. DBS samples prepared from the device were transferred into a 1.5mL Eppendorf tube (polar metabolomics), and another 1.5mL Eppendorf tube (lipidomics). One hundred microliters of chilled methanol was used to extract polar metabolites, with 100uL 1:1 butanol:methanol used to extract lipidic components. Targeted polar metabolite analysis was performed using a Shimadzu GCMS-TQ8040 (QQQ), while untargeted lipidomics analysis was completed using a Shimadzu LCMS 9030 (Q-TOF). Hundreds of metabolites were detected from one dried blood spot, including: glycolytic and TCA cycle intermediates, nucleosides, sugars, fatty acids, amino acids, organic acids. Similarly, hundreds of lipids were detected from one DBS, including classes: acyl carnitine, acylglucuronosyldiacylglycerol, bismonoacylglycerophosphate, cholesteryl ester, diacylglycerol, digalactosyldiacylglycerol, diacylglyceryltrimethylhomoserine, glucuronosyldiacylglycerol, hemibismonoacylglycerophosphate, ceramide, lysophopatidylcholine, lysophosphatidylethanolamine, monogalactosyldiacylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylserine, sphingomyelin, sphinganine, sphingosine, sulfoquinovosyl diacylglycerol, triacylglycerol.
<figure><img src="image_92.png" /></figure>

**POSTER SESSIONS 1 AND 2 – Monday and Tuesday –** all odd number posters will be on display.

**POSTER SESSIONS 3 AND 4 – Wednesday and Thursday –** all even number posters will be on display.

*AWARD WINNERS

TECHNOLOGY

P-580 Exploring Plant Alkaloid Biosynthetic Pathways using Untargeted Metabolomics in Yeast

PRESENTING AUTHOR: Andreas Klitgaard, Novo Nordisk Foundation Center for Biosustainability, DTU, Denmark

CO-AUTHORS: Lars Schrubbers, Mette Kristensen, Jie Zhang, Michael Krogh Jensen and Hanne Bjerre Christensen

Plants are known producers of most potent human therapeutics as well as other industrially relevant molecules. Despite this wealth of compounds, very few products are commercially available, because of low production levels in the plants, as well as high price of production. The alkaloids are secondary plant metabolites that exhibit a remarkable structural diversity with several thousand alkaloids derived from a common precursor. Many of these also exhibit pharmaceutically valuable biological activities, which makes the large-scale production using recombinant microbial cell factories very attractive. Reconstitution of alkaloid biosynthetic pathways in a heterologous host is a proven strategy for rapid and inexpensive production of complex molecules. Baker's yeast Saccharomyces cerevisiae is one of the most common choices for a heterologous microbial host for plant metabolite production because of the abundance of available genetic tools for these organisms. However, a deep understanding of cellular metabolism is essential for their development. In this study, a Thermo Scientific ID-X mass spectrometer was used to investigate the cellular metabolism and to provide valuable insights into the changes associated with genetically modified biochemical reactions and metabolic pathways. Compound annotation was aided by use of the AcquireX workflow, enabling automatic selection and generation of target lists for LC-MS/MS analysis. Data Analysis was performed using Compound Discoverer 3.0, allowing for easy integration of LC-MS and LC-MS/MS data, and rapid compound annotation using a variety of different compound databases.

P-581 Semi-targeted UHPLC-MS metabolomic approaches: A way to make our life easier!?

PRESENTING AUTHOR: Lukáš Najdekr, Phenome Centre Birmingham, University of Birmingham, United Kingdom

CO-AUTHORS: Lukáš Najdekr, Andrew Southam, Andris Jankevics, Martin R. Jones, Thomas Lawson, Giovanny Rodriguez Blanco, William Nash, Elliott Palmer, Mark R. Viant, Ralf J. M. Weber, Warwick B. Dunn

A key challenge in mass spectrometry (MS) untargeted metabolomics is the annotation or identification of metabolite peaks. Metabolite identification must be performed, often manually, for each study individually which is time-consuming. Thus, there is a need for new and novel approaches to allow rapid identification of metabolites using MS, MS/MS and chromatographic retention time (RT) data. One way to address this bottleneck is through semi-targeted metabolomics. This involves the generation of a database of authentic chemical standards – containing RT and MS/MS data – collected using standardised ultra-high performance liquid chromatography-high resolution mass spectrometry (UHPLC-MS) methods. Here, we present three UHPLC-MS assays designed for the analysis of analytical batches of thousands of biological samples (serum, urine, tissue extracts). For the analysis of serum and tissue extracts, two complementary analytical assays (HILIC, C18 reversed phase) enable large coverage of the metabolome and lipidome. For urine, complementary HILIC and C18(aq) reversed phase assays are used. The data are acquired applying untargeted assays and a large proportion of the detected metabolites are automatically fully annotated/identified, which allows much more direct biological interpretation. Additional unidentified peaks are also collected and retained for statistical data analysis. Across the three assays, we have generated a database approaching a 1000 chemical standards containing the RT, full-scan MS and MS/MS data. Matching of the chemical standards to the biological data is performed using an automated data processing workflow. All analytical methods and data processing workflows will be made open-source to maximise the value to the wider scientific community.

P-582 Towards kit-based urinary metabolomics by GCxGC-TOFMS

PRESENTING AUTHOR: James Harynuk, University of Alberta, Canada

CO-AUTHORS: Michael D.S. Armstrong, O. Rene Arreondo-Campos, A. Paulina de la Mata

Comprehensive two-dimensional gas chromatography (GCxGC-TOFMS) is a valuable tool for metabolomics research. It offers vastly improved separation of components over one-dimensional methods, yielding purer spectra for compound identification and purer peaks for quantification. The major challenge in using GCxGC-TOFMS for quantitative metabolomic work lies in how to reliably perform and maintain instrument calibration for a large number of target compounds in a cost-effective and timely manner. In this pilot study, we use a group of 60 metabolites to evaluate a protocol for instrument calibration and for standard preparation that is easily adaptable to preparing suites of standards, potentially containing hundreds of analytes. Ultimately this will lead to sample preparation and calibration kits for performing urinary metabolomics studies by GCxGC-TOFMS, similar to those available for one-dimensional GC-MS.
POSTER SESSIONS 1 AND 2 – Monday and Tuesday – all odd number posters will be on display.

POSTER SESSIONS 3 AND 4 – Wednesday and Thursday – all even number posters will be on display.

*AWARD WINNERS

TECHNOLOGY

P-583 Analysis of Lipid and Fatty Acid Isomers Using a Travelling Wave Cyclic Ion Mobility Separator

PRESENTING AUTHOR: David Heywood, Waters Corporation, United Kingdom

CO-AUTHORS: Michael McCullagh, Martin Palmer, Russell J Mortishire-Smith, Johannes PC Vissers, Chris Hughes, James I Langridge

Data were collected on a research platform based upon a SYNAPT G2-Si instrument where the standard T-Wave IM device is replaced by a cyclic ion mobility (cIM) separator. The cIM has multiple benefits: the circular path minimises instrument footprint whilst providing a longer, higher mobility resolution separation path; a multi-pass capability provides significantly higher IM resolution over a reduced (selected) mobility range; the device can be enabled for mobility separation or by-passed and the ion entry/exit array can selectively eject species. The cIM device consists of a 100 cm path length RF ion guide which provides mobility separation. MS and tandem MS data were obtained on precursor and product ion separated lipids, respectively, followed by TOF mass measurement. Unsaturated free fatty acid (FA) standards, differing in chain length and number of cis/trans conformations, were chosen to determine the degree of IM separation required to separate lipid isomers. FAs represent the simplest class of lipid components and are a core structural component of each lipid category. In direct infusion cIM-MS measurements FAs with cis-double bond orientations, introduced as two component mixtures, were found to be more compact than those with trans-orientations. Moreover, the cis- and trans-orientations for the monounsaturated FA's were distinguishable. A different number of cycles through the cIM separator were required to achieve a similar degree of IM separation for mono unsaturated FAs of differing chain length. Unsaturated FAs with two or more double bonds, separated by two mid-chain carbons, could not be distinguished.

P-584 Automated sample preconcentration into a (sub)microliter droplet using vision-controlled evaporation

PRESENTING AUTHOR: Paul Miggels, Leiden University, Netherlands

CO-AUTHORS: Paul Vulto, Isabelle Kohler, Thomas Hankemeier

Analysis of volume- or biomass-limited samples, e.g. micro-dialysates, cerebrospinal fluid or cell culture extracts, has become essential for the discovery of new metabolite biomarkers. This, however, faces two significant challenges: i) low limits of detection required for low-abundant metabolites, and ii) dilution of small samples (<2 µL) during sample collection, handling and preparation. This study presents a novel preconcentration approach based on vision-controlled solvent evaporation from a constant-volume pendant droplet. This system is integrated in a commercial robotic autosampler for direct coupling to state-of-the-art LC-MS instrumentation. As the sample is dispensed from the autosampler's syringe, a pendant droplet forms at the needle tip inside a special evaporation chamber. The droplet volume is continuously monitored and maintained constant by adjusting the evaporation rate accordingly with a PID feedback loop. The chamber dimensions, temperatures and gas flow for evaporation have been optimized, achieving the preconcentration of 20 µL samples into 0.5-1.0 µL droplets in 3-5 minutes for various mixtures of water with common organic solvents. Repeatability has been evaluated for three model metabolites, i.e. leucine, tryptophan, and valine (N=4). Furthermore, 20-fold preconcentration of cell extracts, cell medium, and plasma samples was successfully demonstrated. By keeping the analytes in suspension during the preconcentration process, and making use of the self-cooling effect of evaporation, this technique is especially suited for volatile and thermosensitive metabolites. This novel approach opens new perspectives for high-throughput and exhaustive metabolomics analysis.

P-585 Ozone-Based Methods for Improved IM-MS Separations and Identification of Metabolites and Lipids

PRESENTING AUTHOR: Christopher Chouinard, Florida Institute of Technology, United States

CO-AUTHORS: Robert Fraser-Caris, Kristie Baker, Samuel Maddox

Ion mobility-mass spectrometry (IM-MS) has seen increased use in the fields of metabolomics and lipidomics during the last decade. In addition to the upsurge in commercially available IM-MS instrumentation, this increased use is primarily a result of the numerous advantages afforded by the technique: structure-based separations allowing for differentiation of isobars/isomers, measurement of collision cross section (CCS), etc. Despite these advantages, IMS separations remain challenged by limited resolution and inability to definitively identify unknown compounds in complex mixtures. This presentation will focus on ozone-based methods as a simple, cost-effective strategy for improving difficult IM-MS separations. Ozone-induced dissociation (OzID) is an established method for cleavage of C=C double bonds, allowing for precise determination of such bonds within fatty acid tails. Ozone-induced rearrangement (OzIR) is a recently introduced technique that targets C=C double bond-containing rings, such as those founds in steroids, prostaglandins, etc. Rather than producing m/z-specific fragments, OzIR changes the overall structure of a molecule, allowing for differentiation by IMS and MS/MS. This strategy has been successfully applied to several molecular classes, including isomeric prostaglandins A1 and B1. These structurally similar compounds differ only in the position of their double bond, and as such their MS/MS patterns are very similar and their CCS are nearly identical (187.0 and 185.4 Å2, respectively). However, following OzIR these isomers produce different fragmentation spectra and have a more than threefold increase in their ΔCCS, allowing effective IM-MS/MS differentiation. Herein we present improvements in metabolomics/lipidomics analyses using ozone-based methods.
POSTER SESSIONS 1 AND 2 – Monday and Tuesday – all odd number posters will be on display.

POSTER SESSIONS 3 AND 4 – Wednesday and Thursday – all even number posters will be on display.

*AWARD WINNERS

TECHNOLOGY

P-586 Development of more reproducible and sensitive polar metabolomics methods

PRESENTING AUTHOR: Steven Fischer, Agilent Technologies, United States

CO-AUTHORS: Sara Violante, Hardik Shah, Yuqin Dai, Justin R. Cross

Ion-pairing reverse phase chromatography (IP-RPC) is a good separation technique for polar metabolites, but its usefulness is often constrained by signal suppression. Here, we reduce the signal suppression seen in IP-RPC using post-column additions that increase ionization efficiency, resulting in improved detection of metabolites over a wide dynamic range and from a single run. Cell extracts (HEK293 cells) and metabolite standards were analyzed using an IP-RPC method, with or without post-column chemistries, with a focus on chromatographic performance and signal suppression. The post-column chemistries consisted of acetone and dimethyl sulfoxide (negative mode) or diethylene glycol monomethyl ether (positive mode), added to the column effluent separately or together. Mobile phases and chromatographic gradients were optimized for each method considering several classes of biologically important compounds. Adding post-column chemistries increased the sensitivity of IP-RCP from >2-5 fold response. The highest increases in response were seen in nucleotides and other phosphorylated compounds in negative mode, as well as polyamines and B vitamins in positive mode. This required a 9:1 blend of acetone and DMSO (negative mode) or acetone and diethylene glycol monomethyl ether (positive mode) and neither solvent achieved this effect when used alone. Other solvent blends were also tested with some success, but the effect on sensitivity was restricted to specific classes of compounds. We believe this increased sensitivity means IP-RPC should be considered more routinely for the separation and analysis of polar metabolites.

P-587 Improved High-throughput Targeted Lipidomic Analysis with sMRM Pro Builder

PRESENTING AUTHOR: Santosh Kapil Kumar Gorti, SCIEX, United States

CO-AUTHORS: Sean Seymour, Mackenzie J Pearson, Christie Hunter, Paul RS Baker

Isomer interference among different lipid classes is known as one of major challenges for application of LC-MS/MS methods for lipidomic analysis. Often, one lipid molecule can have multiple isomers and lipid classes sharing the identical precursor and product masses. To overcome this issue, an Amide chromatographic method is implemented to provide LC separation of lipid classes. In order to confirm separation efficiency, lipids standards (one standard per lipid class) were injected individually to confirm that there was no isomer crosstalk among different lipid classes. Preliminary data is collected with no MRM scheduling on a representative matrix (with or without internal standards) for any biological study. Results from this unscheduled MRM datasets are entered into the sMRM Pro Builder and an initial rough approximation of the retention times is determined. Next replicate injections are performed on the matrix using a preliminary retention time scheduled MRM method, ideally with a pooled sample from all the biological samples. Data analysis here provides information on peak width, RT variance, lipid signal, then the sMRM Pro Builder computes a final optimized time scheduled MRM method. Excellent reproducibility was observed with majority of lipid species showing retention time standard deviations below 0.05 minutes. Lipid standards from 19 different classes, which are either heavy isotopic labeled lipids or odd chain lipids, were used as internal standard. This method provided extensive lipid class coverages including, CE, CER, DCER, HCER, LCER, TAG, DAG, MAG, LPC, PC, LPE, PE, LPG, PG, LPI, PI, LPS and PS.

P-588 Improving lipid annotation coverage using intelligent precursor selection software on an Orbitrap-based mass spectrometer

PRESENTING AUTHOR: Elizabeth Crawford, Thermo Fisher Scientific GmbH, Germany

CO-AUTHORS: Sven Hackbusch, David Peake, Reiko Kiyonami

Lipid profiling provides valuable information to identify disease states and other physiological changes. A common approach used in lipid profiling by LC-MS is to identify lipid species by their MS/MS spectra prior to extraction of precursor information for relative quantitation between conditions. The novel intelligent data acquisition strategy, AcquireX, on the Thermo Scientific™ Orbitrap ID-X™ Tribrid™ mass spectrometer automatically excludes background ions from the MS/MS acquisition and prioritizes MS/MS precursor selection on sample relevant compounds, resulting in deeper lipidome coverage. Here, Bovine liver total lipid extract (Avanti Polar Lipids) was used to demonstrate the utility of the workflow. Samples were separated using reversed-phase conditions on a C30 column with mass spectral data acquired on an Orbitrap ID-X Tribrid MS. Data analysis was performed using Thermo Scientific™ LipidSearch™ 4.2 software to identify lipid molecular species based on acquired MSn fragmentation spectra. The AcquireX data acquisition strategy was used to automatically generate background exclusion and/or compound inclusion lists that were updated iteratively for replicate injections, reducing redundant data collection and triggering more unique lipids for fragmentation. As a result, a significantly larger number of lipids (>40%) could be detected compared to a conventional data-dependent MS/MS approach. In addition, the acquisition of neutral loss-triggered CID-MS3 and product ion-triggered CID-MS2 improved the confidence in the lipid annotations. For example, the use of fatty acid neutral loss-triggered MS3 fragmentation with triglycerides allowed to distinguish multiple co-eluting isomers with different fatty acid chain lengths based on their respective MS3 data.
<figure><img src="image_93.png" /></figure>

**POSTER SESSIONS 1 AND 2 – Monday and Tuesday –** all odd number posters will be on display.

**POSTER SESSIONS 3 AND 4 – Wednesday and Thursday –** all even number posters will be on display.

*AWARD WINNERS

TECHNOLOGY

P-589

Intelligent Acquisition for Comprehensive Metabolome Coverage in Plants, Mammals, and Bacteria

PRESENTING AUTHOR: Tatjana Talamantes, Thermo Fisher Scientific, United States

CO-AUTHORS: Sven Hackbusch, Ioanna Ntai, Amanda Souza

Compound identification remains a challenge in untargeted metabolomics. In LC/MS based untargeted metabolomics experiments, the detection of thousands of features in a single sample is routinely accomplished. However, this should not be equated to "global" metabolome coverage, as only a small percentage of those metabolites are of biological origin. Data-dependent acquisition (DDA) often provides information for the most abundant ions. Recently developed AcquireX acquisition software can determine on-the-fly features corresponding to background contaminants and compound degeneracy, such as isotopes, adducts, and dimers, enabling more efficient MS/MS and MSn sampling of unique biologically relevant metabolites. Unlike traditional DDA, during which the fragmentation of background ions dominates the duty cycle, the AcquireX workflow selects precursors intelligently by excluding background ions and targeting unique metabolites of biological relevance for fragmentation. Here, we used samples of varying matrix and complexity, to demonstrate the utility of AcquireX acquisition across several sample types. By excluding background and degenerate signals, the total number of fragmentation targets was reduced without compromising metabolite coverage. By focusing acquisition on biologically relevant compounds, more time could be spent collecting multistage (MSn) fragmentation data, without affecting experiment length. MSn provided additional structural information and confidence for compound annotations and, in the case of flavonoids, isomeric compound annotation candidates could be differentiated without the need for additional experiments. Ultimately, AcquireX intelligent acquisition enabled annotation of non-biological and redundant features on-the-fly, resulting in comprehensive MSn coverage regardless of sample type, complexity, and concentration. For Research Use Only. Not for use in diagnostic procedures.

P-590

Development of a Collision Cross Section Library using Trapped Ion Mobility Spectrometry (TIMS) and Its Use in Plant Metabolomics

PRESENTING AUTHOR: Mark Schroeder, University of Missouri - Columbia, United States

CO-AUTHORS: Sven Meyer, Aiko Barsch, Lloyd W. Sumner

The timTOF has been frequently used in the analysis of proteomics and is now gaining increased interest for small-molecule metabolomics. To evaluate the potential benefits the TIMS technology can provide, we have measured the CCS values of plant specialized metabolites such as flavonoids and saponins. These compounds are structurally diverse due to various flavonoid and triterpene aglycones as well as various modifications to the aglycones such as hydroxylation and glycosylation. We recorded CCS values for over 150 specialized metabolites. Specifically, the samples were analyzed in triplicate and the average, standard deviation, and relative standard deviation (RSD) of CCS values were recorded. RSD of <1.0% were obtained for all compounds. In addition, we annotated adduct formations of the metabolites which are commonly observed in LC-MS analysis. To test the robustness of the CCS measurements and matching of library compounds to analytes we further tested a standard mix of 5 compounds from our library. We pooled 5 flavonoids and glycosylated flavonoids and analyzed with and without a plant compound matrix through direct infusion TIMS-QTOFMS and UHPLC-TIMS-QTOFMS. Then we compared the recorded CCS values between the various methods and the recorded library. We found the CCS measurements and the library were consistent and the compounds could be reproducibly detected and identified during data processing. The usefulness of the CCS library was demonstrated in the analysis of Medicago truncatula and Glycine max extracts. Results indicated that the CCS library allowed for more confident identifications of flavonoids and saponins with improved annotation quality scores.

P-591

Simultaneous analysis of SIM and Scan mode in a single run using LC-QTOFMS: Comprehensive cell culture profiling of iPS cell

PRESENTING AUTHOR: Takanari Hattori, Shimadzu Corporation, Japan

CO-AUTHORS: Toshiya Matsubara, Tsuyoshi Nakanishi, Jun Watanabe

Culture medium is composed of various biologically important compounds such as vitamins, amino acids, nucleic acids and other primary metabolites. Comprehensive analysis of these compounds would lead to more detailed understanding of the bioprocess. We report comprehensive cell culture profiling of iPS cell by metabolomics based LC-QTOFMS. SIM and Scan mode were simultaneously used in a single run. Feeder-free iPS cells (1231A3) were maintained in AK02N medium for 6 days. Proteins were removed from the supernatants by adding acetonitrile and centrifugation. The supernatants were analyzed after dilution with ultrapure water. NexeraTM X2 system coupled with a LCMSTM-9030 (Shimadzu Corporation, Japan) was used. MS analysis consisted of SIM and Scan mode in positive mode. 68 compounds were targeted for SIM mode. MS spectra were acquired from m/z 50 to 500 for Scan mode. As a result of targeted analysis using SIM mode, 27 compounds such as amino acids and vitamins were detected with high sensitivity. Alanine, kynurenine, ornithine and few compounds have increased with time course. In contrast, arginine, methionine, tryptophan and few compounds have decreased with time course. As a result of untargeted analysis using Scan mode, some unknown compounds were detected and their amount have increased or decreased with time course. Accurate mass, database (Human Metabolome Database) and MS/MS spectrum were used to identify unknown compounds and N'-formylkynurenine was identified as one of unknown compounds. N'-formylkynurenine is intermediate metabolite between tryptophan and kynurenine in kynurenine pathway. N'-formylkynurenine have increased with time course.
**POSTER SESSIONS 1 AND 2 – Monday and Tuesday –** all odd number posters will be on display.

**POSTER SESSIONS 3 AND 4 – Wednesday and Thursday –** all even number posters will be on display.

*AWARD WINNERS

TECHNOLOGY

**P-592**
**HILIC-HR-MS for (untargeted) metabolomics in microorganisms – the optimal method for polar compounds in an industrial setting?**

**PRESENTING AUTHOR:** Leon Coulier, DSM Biotechnology Center, Netherlands

**CO- AUTHORS:** Wouter Coppes, Reza Maleki-Seifar, Sandra Pous-Torres, Raymond Ramaker

The analysis of polar intracellular metabolites, e.g. amino acids, organic acids, sugar phosphates and nucleotides, is always a challenge. Although for each class of compounds separate methods are available, there are not many methods that can cover all these classes at once. One of the methods that can do this is ion-pair liquid chromatography. Ion-pair LC gives very good retention for polar metabolites on C18 columns. The major drawback of the use of an ion-pair agent is the contamination of the MS, especially when MS instruments are shared and used for different purposes. This often means that the only option to use ion-pair LC is having a dedicated LC-MS system that is only used for this purpose. However, this might not always be possible and when possible, there is not always high-end LC-MS equipment available. There was therefore a big need in our lab to find a good alternative for ion pair LC. The aim was to have a method that is compatible with high-end HR-MS equipment. Hydrophilic interaction liquid chromatography (HILIC) is often proposed as good alternative and many papers have described the possibilities of HILIC for the analysis of polar intracellular metabolites. In this poster we will describe our search for the best HILIC-HR-MS method for intracellular polar metabolites that could replace our ion-pair LC-MS method. Different columns, buffers, pH etc. were studied and, very important, the method was tested using real-life complex matrices and samples.

**P-593**
**A high throughput liquid chromatography-mass spectrometry metabolomics method and its application in early warning of diabetes**

**PRESENTING AUTHOR:** Yang Ouyang, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, China

**CO- AUTHORS:** Xinjie Zhao, Guowang Xu

Metabolomics has been widely applied in clinical study recently. For conventional metabolomics methods based on liquid chromatography-mass spectrometry (LC-MS), procedures of pretreatment and separation are time-consuming. And it directly influences the sample throughput. To fulfill the requirement of the large-scale sample analysis, a high throughput metabolomics method was established by using an efficient 96-well-plate-based sample preparation protocol and a short 12-min LC gradient, which reduced about 70% of the sample processing time and 60% of the analysis time. Evaluation of analytical performances including the coverage of metabolites reflected that the method was robust for the large-scale metabolomics study. To show the usefulness, in a nested case-control study comprising 30,000 Chinese participants, subjects with new-onset diabetes in five years were selected as a case group (n=295), matching the subjects of the healthy group with no diabetes in the same period according to clinical information such as age and gender (n=295). Using this high throughput method, we found that amino acids, acylcarnitine and unsaturated free fatty acids accumulated in the case group, while the amount of lyso-phosphatidylcholine species decreased. And differential metabolites' combination also achieved a suitable discriminating power between the control and case. Therefore, our research has the potential to provide a research basis for the early warning of diabetes. Moreover, the high throughput method has a wide application prospect in the field of large-scale metabolomics study.

**P-594**
**Data Stability of GC-FID in Metabolomics**

**PRESENTING AUTHOR:** Takero Sakai, Shimadzu Corporation, Japan

**CO- AUTHORS:** Yusuke Takemori, Kiyomi Arakawa

One of the most important and difficult problem in Metabolomics is data stability. In GC-MS Metabolomics, the data would be unstable as time passed after TMS derivatization, that might be caused by MS tuning, pollution, and the stability of derivatized compounds. Therefore in GC-MS, data acquisition with long time span may sometimes need some modification to the data, such as correction with quality control sample. GC-FID is a conventional analytical instrument that is useful for many fields. However GC-FID might be less utilized in Metabolomics than GC-MS so far. This is because GC-MS is more powerful in peak annotation and peak annotation is considered necessary for Metabolomics. But GC-FID has a massive feature for Metabolomics; data stability. We prepared commercial product of beer as samples and try to analyze it with GC-FID. The stability of the obtained data within 60 hours were incredibly better than GC-MS. Moreover, we stored the extraction of the samples with dryness and analyzed it after 2 weeks. The obtained data after 2 weeks were almost identical not only as chromatogram shape but also peak area. Under these background data, we tried to classify the subtle beer samples that were the same as product but differentiate with their lots and manufacturing place. With these result, we suggest that GC-FID has an underrated potential for Metabolomics, especially for classification / regression of samples with big data that needs more stable data acquisition system than MSs.
**POSTER SESSIONS 1 AND 2 – Monday and Tuesday –** all odd number posters will be on display.

**POSTER SESSIONS 3 AND 4 – Wednesday and Thursday –** all even number posters will be on display.

*AWARD WINNERS

TECHNOLOGY

**P-595** Chemical isotopic labeling of multiple functional groups in urine metabolome

PRESENTING AUTHOR: Cheng-Yu Hung, Chang Gung Molecular Medicine Research Center, Taiwan

CO-AUTHORS: Cheng-Yu Hung, Ya-Ju, Hsieh, Jau-Song Yu, Yi-Ting Chen

A high proportion of metabolites in human urine possess hydroxyl and amino groups. Chemical Isotope Labeling (CIL) is useful for improving the poor ionization efficiency and low existing level in biological fluids by liquid chromatography-mass spectrometry (LC-MS). Dansyl chloride (DnsCl) has been widely reported as a derivatizing reagent for facilitating the MS detection. We described a developed dansylation method for improving the mass spectrometry detection of the amine/phenol and the hydroxyl metabolites in the urine samples. To optimize the whole workflow, we measured with five reaction factors including sample diluted factors, reaction volume, reaction time, reaction temperature, and molar ratio of DnsCl to catalyst. A urinary pooling was labeled with 12C- or 13C-DnsCl on a base-activated reaction. Chemical standards of amino acid and hydroxyl compounds were constructed for positive control. After incubation at 65 for one hour, the equal mole amount of 12C- / 13C-labeled mixtures in triplicate were analyzed the peak pairs of labeled metabolites using LC-MS. Furthermore, we also compared this developed method with other dansylation protocols. In summary, this technique improves the detection for labeling both amino group and hydroxyl group simultaneously.

**P-596** MetaboLights study editor - An open-access curation tool for metabolomics studies submission and associated meta-data annotation

PRESENTING AUTHOR: Venkata Chandrasekhar Nainala, The European Bioinformatics Institute (EMBL-EBI), United Kingdom

CO-AUTHORS: Claire O'Donovan, Jiakang Chang, Jose Ramon Macias, Kalai Vanii Jayaseelan, Keeva Cochrane, Kenneth Haug, Namrata Kale, Pablo Moreno, Rachel Spicer, Mark Williams

MetaboLights database is an international metabolomics repository recommended by many leading journals including Nature, PLOS and Metabolomics. The service's unique manual curation maintains quality, provides helpful support for users and ensures accessibility for secondary analysis of studies. MetaboLights hosts a wealth of cross-species, cross-technique, open access experimental research. As a part of our ongoing efforts to streamline the study submission and curation process, the MetaboLights team at EMBL-EBI has developed a new tool to submit and edit studies online. This submission tool provides MetaboLights users and curators with an intuitive and easy to use interface to create, edit and annotate their studies online. The convenient, context-aware editor navigates users through the study to define a rich description of the experimental metadata including study characteristics, protocols, technology and related factors. Metadata descriptions are enhanced by mapping this information to controlled ontologies repositories using ZOOMA, capturing such a complete data set benefits the community by making results findable, reproducible and reusable. Going forward we have plans to incorporate text mining tools such as Named Entity Recognition (NER) to annotate metadata, enabled by the robust architecture of the online editor. Other ideas include offline edit support, direct channels for curators to contact and communicate with the submitters to make the whole process of data curation more submitter-friendly.

**P-597** Effects of Deuterium Oxide (D2O) in 1H NMR Metabolomics

PRESENTING AUTHOR: Kristina Haslauer, Helmholtz Zentrum Munich, German Research Center for Environmental Health, Germany

CO-AUTHORS: Daniel Hemmler, Philippe Schmitt-Kopplin, Silke Heinzmann

Sample preparation in urine NMR metabolomics generally requires only few steps, however, preparation protocols still have not reached consensus. Most variability in the protocols is given by varying D2O concentrations as locking substance in buffer systems and temperature handling during dwell time prior to measurement. We investigated the effect of deuterium oxide concentrations on urine metabolites for different temperature conditions and found a successive decrease in creatinine peak area up to 35% after 24 h. Creatinine is known to be excreted in a constant rate over 24 h in healthy individuals and therefore frequently used as normalization factor for urinary dilution. Furthermore, creatinine is used as important biomarker for renal function. To address this loss in peak area, a systematic investigation on the underlying mechanism and the impact was carried out in this study. A proton-deuterium (H/D) exchange at the CH2 position was revealed by 1H, (IG) 13C, DEPT-HSQC NMR and MS experiments leading to this loss. Furthermore, we conducted a sample stability examination for different D2O concentrations and temperatures up to 24 h. We propose an equation to correct the creatinine loss for biobank samples, which was validated on an external dataset, as well as a general guideline for future studies to ensure a high creatinine stability.
**POSTER SESSIONS 1 AND 2 – Monday and Tuesday –** all odd number posters will be on display.

**POSTER SESSIONS 3 AND 4 – Wednesday and Thursday –** all even number posters will be on display.

*AWARD WINNERS

TECHNOLOGY

P-598

Impact of pre-analytical sample handling on plasma for 1H-metabolomics

PRESENTING AUTHOR: Anders Pedersen, Swedish NMR Centre at the University of Gothenburg, Sweden

CO-AUTHORS: Daniel Malmodin, Huma Zafar, Åsa Torinsson Naluai, B. Göran Karlsson

The pre-analytical sample handling of plasma samples prior to freezing has an unwanted impact on resulting metabolite concentrations in bio-banked samples. It is important to quantify how various metabolites are affected depending on handling since it otherwise risk to bias studies when e.g. study samples and controls have not been treated equally. Using 1H NMR spectroscopy we have investigated the effect on the metabolite profile by exposing plasma samples to light or no light, three different temperatures and different times prior to centrifugation. While light or no light does not influence the metabolite concentrations, temperature and time to centrifugation does, in a predictable way. Therefore, it would have been preferable if temperature and time to centrifugation values had been stored when samples were collected. Since this usually is not the case, an alternative approach is to estimate these parameters from relative concentration differences between specific temperature and time sensitive metabolites. We present sensitive metabolites and outline how their concentrations can be used to estimate the exposure to temperature and time to centrifugation for a given sample.

P-599

Development of a Metabolomics System Suitability Sample for MS-based Metabolomics

PRESENTING AUTHOR: Tracey Schock, National Institute of Standards and Technology, United States

CO-AUTHORS: Deb Ellisor, Clay Davis

Prior to the actual analysis of biological samples, one must first assess the suitability of the analytical instrumentation. In this regard, the metabolomics community is suffering from the lack of an everyday system suitability standard by which to benchmark instrument performance for untargeted MS based approaches. A complex solution that mimics biological samples is required to determine whether the analytical run is of acceptable quality and to ensure lack of contamination prior to experimental analyses. Individual laboratories use a small number of standards (5-15) to create in-house suitability solutions for assessing measurement quality of hundreds to thousands of chemicals profiled in an untargeted study of incredibly complex samples. Over-reporting and spurious conclusions are likely rampant in the literature due to the lack of a material to evaluate measurement quality across a complex omics profile. NIST is developing a large quantity, biological extract from human liver which incorporates the entirety of a metabolome, resulting in a more encompassing system suitability sample. The design of a tissue extract as a suitability standard eliminates sample preparation variation observed with biological samples while offering simplicity of use and analyte complexity for analysis of metabolomics platforms. Additionally, the extract can be a tool in harmonization of instrument performance in large, multi-center studies. NIST candidate research grade material (RGM) 10122 Metabolomics System Suitability Sample will be assessed for homogeneity and stability of constituent metabolites. The sample is available pre-sale for those interested in evaluating the material for the metabolomics community's needs.

P-600

mQACC: A community-led initiative to promote quality assurance and quality control in untargeted metabolomics research

PRESENTING AUTHOR: Krista Zanetti, National Cancer Institute, United States

CO-AUTHORS: Fadi Abdi, Abbas Bandukwala, Aiko Barsch, Dan Bearden, Richard Beger, Bianca Bethan, David Broadhurst, Clary Clish, Surendara Dasari, Leslie Derr, Suraj Dhungana, Warwick Dunn, Tim Ebbels, Annie Evans, Steve Fischer, Roberto Flores, Thomas Flynn, Charles Grieser, Thomas Hartung, Majda Haznadar, David Herrington, Rick Higashi, Ping-Ching Hsu, Christina Jones, Judith Jans, Maureen Kachman, Jennifer Kirwan, Andre Kleensang, Matthew Lewis, Katrice Lippa, Padma Maruvada, Sven Meyer, Maria Eugenia Monge, Jonathan Mosley, Ioanna Ntai, Claire O'Donovan, George Papanicolaou, Rui Pinto, Mary Playdon, Dan Raftery, Sharon Ross, Michael Schmidt, Tracey Schock, Amanda Souza, Jinchun Sun, Fariba Tayyari, Georgios Theodoridis, Frederico Torta, Baljit Ubhi, Vidya Velagapudi, Mukesh Verma, Mark Viant, Dajana Vuckovic, Li-Rong Yu, Tilmann Walk, Ian Wilson

The metabolomics Quality Assurance and quality Control Consortium (mQACC) is a community-led initiative to promote quality assurance (QA) and quality control (QC) in untargeted metabolomics research. mQACC was established through a National Institutes of Health-funded meeting in October 2017. Data from a survey published by Dunn, et al. Metabolomics, 2017) was used to establish meeting objectives: 1.) identify the most useful metrics for assessing study and data quality for untargeted metabolomic studies; 2.) identify and prioritize processes to ensure appropriate reporting of QA/QC data; and 3.) identify and prioritize the types of test materials that are needed in the field of metabolomics for QA/QC in untargeted studies. Key priorities were identified and scored for importance, resulting in several primary themes. Although priorities were identified for long-term efforts, three immediate priorities were moved forward: 1.) publish a workshop report (Beger et al., Metabolomics, 2019); 2.) document and subsequently publish the complete experimental procedure for untargeted metabolomics; and 3.) identify 2-3 reference materials to be developed quickly. mQACC currently includes over 55 representatives from the North America, South America, Europe, and Asia, including instrument manufacturers, commercial laboratories, and government and academic stakeholders. The consortium is currently addressing their initial priorities, as well as expanding these efforts. mQACC has also established several working groups to achieve their objectives, including ones focusing on experimental procedures, reference materials, QC best practices, and reporting standards. In addition to describing the mQACC priorities, we will highlight how the metabolomics community can get involved in this important initiative.
**POSTER SESSIONS 1 AND 2 – Monday and Tuesday –** all odd number posters will be on display.

**POSTER SESSIONS 3 AND 4 – Wednesday and Thursday –** all even number posters will be on display.

*AWARD WINNERS

TECHNOLOGY

P-601

Metabolomics with Matrigel-based 3D cell culture: analytical challenges

PRESENTING AUTHOR: Janina Tokarz, Helmholtz Zentrum München, Research Unit Molecular Endocrinology and Metabolism, Germany

CO-AUTHORS: Gabriele Möller, Cornelia Prehn, Jerzy Adamski

Cell culture metabolomics is a versatile tool to understand metabolic processes in cells. Two-dimensional (2D) cell culture allows for easy manipulation of cells and is well established in metabolomics experiments. However, cells in 2D encounter a non-physiological environment and are known to loose key functionalities such as their xenobiotic metabolizing capacity. These limitations can be overcome by cultivating the cells in three-dimensions (3D) supported by extracellular matrices such as Matrigel. Our goal was to analyze the feasibility of combining Matrigel-based 3D cell culture with targeted metabolomics. Hep G2 cells were seeded in varying densities into Matrigel. Solidified Matrigel-cell complexes were covered with culture medium. The cell complexes were harvested by scraping them in ice-cold extraction solvent consisting of 80% methanol and subsequently disrupted using bead-based homogenization. After centrifugation, metabolites were analyzed with the Biocrates AbsoluteIDQTM p180 Kit. 2D cultured cells and plain Matrigel samples were analyzed in parallel. We observed significant ion suppression by Matrigel. In addition, some metabolites, mostly lipids, showed higher concentrations in cell than in Matrigel samples. Many amino acids and the hexoses were higher concentrated in Matrigel samples without cells, indicating diffusion of these metabolites from the culture medium into the matrix. Our data demonstrate that targeted metabolomics with Matrigel supported 3D cell culture samples is possible. However, due to the matrix, metabolites provided by culture media remain in the sample despite washing steps. Thus, the extracellular matrix causes a major contamination and remains a challenge for metabolomics experiments.

P-602

Feature-Based Molecular Networking of Non-targeted Mass Spectrometry Data: Bridging MS-DIAL, MZmine2, OpenMS, and XC-MS, with the GNPS web-platform

PRESENTING AUTHOR: Louis Felix Nothias, University of California San Diego, United States

CO-AUTHORS: Louis Felix Nothias, Daniel Petras, Mingxun Wang, Robin Schmid, Abinesh Sarvepalli, Zheng Zhang, Ricardo da Silva, Alexander Aksenov, Pieter C. Dorrestein

Molecular networking has become an essential bioinformatic tool to annotate non-targeted tandem mass spectrometry data (MS/MS). Available on the GNPS web-platform (http://gnps.ucsd.edu), molecular networking accelerates the annotation of molecular/spectral families by propagating spectral library matches across the networks. Based on the MS-Cluster algorithm, molecular networking enables the large scale metabolomics meta-analysis, up to thousands of files. However, as MS-Cluster uses the MS/MS spectral counts as a proxy for the ion distribution between samples, other methods are needed to estimate the relative ion abundance, especially when analysing a single study. In the present work, we introduce feature-based molecular networking (FBMN), a collection of computational tools integrated together into a seamless LC-MS data processing pipeline combined with molecular networking on the GNPS web-platform (https://ccms-ucsd.github.io/GNPSDocumentation/featurebasedmolecularnetworking/). Utilizing software with advanced graphical user interface (MS-DIAL, MZmine2, MetaboScape) or advanced computational libraries (OpenMS, and XC-MS), the workflows serves both experimentalists, bioinformaticians, and software developers. We benchmarked FBMN on dilutions of the NIST serum samples, and results showed that it improves the estimation of ion relative distribution between samples, and reduced considerably the number of spectral artefacts. These results were observed for both Orbitrap and QTOF instruments, and different sample types, indicating that FBMN has significant advantages over MS-Cluster based molecular networking. Nonetheless, the FBMN is proposed to serve as a complementary tool to the "classical", MS-Cluster-based molecular networking which is a robust and nearly parameterless method, and is the only method capable of performing large scale meta-analysis of non-targeted LC-MS/MS metabolomics datasets.

P-603

A comparison of untargeted metabolomic methods applying 2.1 and 1.0 mm ID UHPLC columns for the study of mammalian metabolomes

PRESENTING AUTHOR: Annie Harwood-Stamper, University of Birmingham, United Kingdom

CO-AUTHORS: Lukáš Najdekr, Mark R. Viant, Caroline A. Rowland, Warwick B.Dunn

Untargeted ultra-high-performance liquid chromatography-mass spectrometry (UHPLC-MS) metabolomics has been successfully applied for the study of a wide variety of sample types. Standard methods employ columns with 2.1mm internal diameters (ID) where sample volume is available in sufficient quantities. However, if sample volumes are limited (<10µL), more sensitive analytical methods are required because of the requirements for sample dilution. Previous research has shown that use of a smaller ID column provides the required increase in sensitivity for a limited number of sample types and complete characterization of sensitivity improvements has not been performed (Gray et al., 2016, Gray et al., 2015, Plumb et al., 2004). Therefore, the aim of the study presented here was to determine the influence of column ID on sensitivity and volume of sample required by comparing 2.1mm ID column and 1.0mm ID column methods. Human plasma and urine were prepared in serial dilutions and UHPLC columns of two different IDs (2.1 and 1.0mm) but with the same stationary phase composition and particle size were used to optimize the analysis. Three different analytical assays were used: lipophilic reversed phase C18, aqueous end-capped reversed phase C18 for semi-polar metabolites and hydrophilic interaction chromatography (HILIC) for polar metabolites. The 1.0mm methods were then applied to the analysis of mammalian tear samples which are available in volumes of <10µL. Results show that following optimization, the 1.0mm ID methods are more sensitive than the 2.1mm ID methods for the same sample volume and are appropriate for untargeted mammalian tear analysis.
<figure><img src="image_94.png" /></figure>

**POSTER SESSIONS 1 AND 2 – Monday and Tuesday –** all odd number posters will be on display.

**POSTER SESSIONS 3 AND 4 – Wednesday and Thursday –** all even number posters will be on display.

*AWARD WINNERS

TECHNOLOGY

P-604

A simple strategy to improve data quality in LC-MS-based metabolomics studies of human urine

PRESENTING AUTHOR: Adriana Nori de Macedo, Federal University of Minas Gerais, Brazil

CO-AUTHORS: Rosilene Cristina Rossetto Burgos, Pedro Luis Rocha da Cruz, Helio Tedesco-Silva Junior, Karina Helena Morais Cardozo, Valdemir Melechco Carvalho, Marina Franco Maggi Tavares

An important challenge in metabolomics when using liquid chromatography-mass spectrometry (LC-MS) is the variation of signal intensity during sample analysis, due to accumulation of sample components in the column and front parts of the MS system. Herein, we suggest simple changes to improve data quality in LC-MS-based metabolomics, which were tested for urine samples (n=176) from renal transplant patients. These changes include the use of a divert valve to direct the column effluent to waste at the beginning of the chromatographic separation (0.0-0.8 min) and during column cleanup and equilibration (12.0-20.0 min), combined with a longer column cleanup between injections (6.0 min at 0.6 mL/min, instead of 1.0 min at 0.4 mL/min). The method was tested before and after introducing these modifications by analyzing individual urine samples, quality controls (QCs), blanks, and calibrant solutions using an UPLC (Waters) and QExactive (Thermo), along with Skyline for data monitoring. QCs showed great improvement in peak area repeatability, with relative standard deviations (RSDs) of selected metabolites going from ~60% to ~10% as the divert valve and longer column cleanup were introduced. Similarly, RSDs of peak areas for internal standards spiked into individual urine samples improved from ~40% to ~10%. Moreover, calibrant solutions injected firstly and lastly in the analytical sequence had more comparable signals after introducing the modifications. Therefore, in addition to current protocols of preventative maintenance prior to sample analysis, we recommend the use of a divert valve and extended column cleanup as simple modifications to improve data quality in metabolomics studies.

P-605

High-throughput Targeted Lipidomics Analysis of Dihydroceramide Desaturase-1 (DES1) Knockout Mice

PRESENTING AUTHOR: Mackenzie Pearson, SCIEX, United States

CO-AUTHORS: Santosh Kapil Kumar Gorti, Trevor S. Tippetts, Scott A. Summers

Amide column chemistry was chosen for lipid class separation and minimize isomeric interference. The target list of lipids is comprehensive, covering most major lipid classes and categories, and MRMs were selected to cover lipids containing fatty acids with 14 to 22 carbons and 0 to 6 double bonds. The method is customizable, so new lipid categories, classes and molecular species can be added to the MRMs list. This method provides quantitative measurement of over 1150 different lipid molecular species in a rapid, highly reproducible manner. The sMRM Pro Builder template which was developed to streamline the method optimization process, enables assigning the retention time, optimize dwell weight and set window size per MRM to enhanced coverage and sensitivity of the method. This optimization improved results quality especially on low abundant lipids. Liver and eWAT tissues were harvested from dihydroceramide desaturase-1 (DES1) knockout mice. DES1 is the enzyme responsible for inserting the 4,5-trans-double bond into the sphingolipid backbone causing the dihydroceramide conversion to ceramide. While both of these classes of lipids are lower in abundance in the chosen tissues, this method shows significant changes in these lipid classes in a quantitative manner. However, lipids from another 17 classes were not changing. Lipid standards from 19 different classes, which are either heavy isotopic labeled lipids or odd chain lipids, served as internal standard. This method provided extensive lipid class coverages including, CE, CER, DCER, HCER, LCER, TAG, DAG, MAG, LPC, PC, LPE, PE, LPG, PG, LPI, PI, LPS and PS.

P-606

A novel approach of untargeted plant secondary metabolic profiling and its application to studying resistance-related metabolites in maize

PRESENTING AUTHOR: Zaifang Li, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, China

CO-AUTHORS: Xiuqiong Zhang, Yueyi Xia, Xiaoshan Sun, Wenying Liang, Yaorui Ye, Chunxia Zhao, Xin Lu, Guowang Xu

Secondary metabolites are the end products of complex metabolic pathways in plants. They are widely involved in physiological processes such as growth, development, and defense. Plants fight against biotic and abiotic stress by synthesizing and releasing defensive secondary metabolites. However, comprehensive identifications of secondary metabolites are difficult because of plant chemical diversity, highly species/tissue-specific and time-dependent accumulations. It is necessary to develop a comprehensive analytical method for secondary metabolites. In this study, a novel metabolic profiling method based on ultra-high-performance liquid chromatography-high-resolution mass spectrometry was established and applied to investigate secondary metabolites of maize. A high coverage MS/MS acquisition method was developed by a segmented scan. Then, a multi-informational molecular network was constructed for comprehensive identification of secondary metabolites in maize. The secondary metabolites composition of maize leaves, silk and seeds were investigated using the developed method. A tissue-specific accumulation of secondary metabolites was observed. Herbivore-induced alterations in secondary metabolites of maize silk were studied. We found that a great variation was observed on the secondary metabolism of maize silk after insect attack. The insect-resistant metabolites, benzoxazinones, phytoalexins belonging to terpenes (KB1, KA2, KA3, ZB1 and dolabradiene, etc.), hydroxycinnamic acid amides and its precursor cinnamic acids were significantly accumulated in maize silk. While flavonoids and its flavonoid phytoalexins (maysin) were significantly decreased after the attack. These results show the developed untargeted metabolic profiling method is very useful for novel insights into secondary metabolites defense in crops.
<figure><img src="image_95.png" /></figure>

**POSTER SESSIONS 1 AND 2 – Monday and Tuesday –** all odd number posters will be on display.

**POSTER SESSIONS 3 AND 4 – Wednesday and Thursday –** all even number posters will be on display.

*AWARD WINNERS

TECHNOLOGY

P-607

Deeper Level of Comprehensive Metabolite Quantitation with Data Independent Acquisition

PRESENTING AUTHOR: Baljit Ubhi, SCIEX, United States

CO-AUTHORS: Zuzana Demianova

The major challenge in the field of metabolomics is to accurately identify and quantify hundreds of metabolites in a single run. Recently variable window SWATH acquisition has shown to identify a higher number of metabolites compared to the traditional Data Dependent Acquisition (DDA) approach, thus enabling broader metabolome coverage. Here we have implemented a variable window SWATH acquisition method for enhanced quantitation of selected metabolites using MS/MS, with reduced matrix interferences and improved signal-to-noise. Using MS/MS fragments for metabolite quantitation provides better selectivity, and ultimately increased sensitivity. Variable window SWATH Acquisition provided quality quantitative data for metabolites in complex matrix. Due to many coeluting metabolites in complex matrix, using only the MS spectrum and retention time is often not sufficient for metabolite identification. MS/MS information is necessary to obtain further structural knowledge about the metabolite. Complete full scan MS and MS/MS data is available in every SWATH file for improved ID. In addition, MS/MS quantitation of metabolites often leads to lower detection limits due to significantly improved signal to noise ratios vs MS data. Measuring the whole MS/MS spectrum allows selection of the best fragments for metabolite quantitation. SCIEX OS software combines comprehensive qualitative and quantitative data analysis, making data processing easier and more efficient. SWATH Acquisition on all detectable metabolites is successfully utilized for identification, and accurate MS/MS level quantification of metabolites in urine.

P-608

Automated High-Throughput Flux Analysis of Non-Small Cell Lung Carcinoma Cells Grown in vitro in Two and Three Dimensions

PRESENTING AUTHOR: Agnes Corbin, Nonlinear Dynamics/Waters SAS, France

CO-AUTHORS: Abhishek Jha, Raghav Sehgal, Johannes PC Vissers, Amrita Cheema

Cell lines are widely used to study disease and treatment. Monolayer (2D) cell cultures are commonly used for this purpose. The geometry of these cell cultures is however believed to be inadequate to recreate the growth environment of cells. Spheroid (3D) cell cultures are considered to be more viable in vitro alternatives since they better mimic the in vivo conditions. The different outcomes from 2D and 3D culture studies can therefore have a significant impact on the relevance of experimental findings. H1299 cells were grown as 2D monolayers in Eagle's media, and to generate 3D spheroids, cells were grown in 2% pHEMA dissolved in ethanol. Following sample preparation, the samples were injected onto an LC-oaToF-MS system. Next, the LC-MS fluxomics data were processed with an informatics pipeline that consists of data transfer, data conversion into open source standards, and import of the converted data for peak detection, curation, natural abundance correction and visualization. The analysis of the human non-small cell lung carcinoma cells flux data indicated that the lactate fractional enrichment of the 13C3 isotopologue is significantly higher in spheroid vs. monolayer cell cultures. Since 13C3-lactate is the major isotopologue produced from 13C6-glucose, the observations suggest that glycolysis is significantly upregulated in spheroid cell cultures. Moreover, key intermediates in the TCA cycle had higher 13C2 isotopologues. In turn, synthesis of pyruvate 13C3 isotopologues can be attributed to 13C6-glucose, suggesting a higher contribution of glucose to the TCA cycle via acetyl CoA in spheroid cell cultures.

P-609

Meta-analysis of tracer-biotransformation experiments of exogenic and endogenic isotopically-labeled tracer compounds with LC-HRMS and MetExtract II

PRESENTING AUTHOR: Christoph Bueschl, IFA-Tulln, University of Natural Resources and Life Sciences BOKU, Vienna, Austria

CO-AUTHORS: Bernhard Kluger, Maria Doppler, Jacqueline Meng-Reiterer, Asja Ceranic, Rainer Schuhmacher, Rudof Krska

Tracer-based metabolomics studies have become more popular with the increased availability of isotopically-labeled tracers. By spiking a biological organism (e.g. plants, fungi, mammals) with these labeled tracers, virtually all biotransformation-products can be detected that are newly synthesized under the tested experimental conditions. A powerful workflow for the detection of biotransformation-products from secondary metabolism reactions of either endogenous or exogenous tracers, is the TracExtract workflow. It combines LC-HRMS with the custom-tailored software MetExtract-II for the fully automated and untargeted detection of novel biotransformation-products. In this work, the results of several such studies are summarized. Mainly 13C-labeled tracers were studied with the TracExtract workflow (e.g. the mycotoxins deoxynivalenol, T2/HT2 and zearalenone in wheat ears, tryptophan and phenylalanine in wheat and grapes, methylation artifacts during sample preparation, glucose for the detection of RNA-modifications). For example, 172 biotransformation-products of phenylalanine in wheat plants infected with Fusarium graminearum, more than 50 novel detoxification products of mycotoxins in different cereal plants, and 88 sampling artifacts of methanol were successfully detected. The number of identified metabolites varied greatly (10-172) depending on the studied tracers. No modifications of the general workflow or LC-HRMS setup were required for the majority of the performed experiments. The automated, untargeted search for novel biotransformation products was very specific in all experiments and resulted in only few (1-5) false-positives on average. The results demonstrate that isotopically-labeled tracers and the developed workflow are generic, highly specific and can be applied to virtually any isotopically-labeled tracer as there is no need for complicated experimental setups.
<figure><img src="image_96.png" /></figure>

**POSTER SESSIONS 1 AND 2 – Monday and Tuesday –** all odd number posters will be on display.

**POSTER SESSIONS 3 AND 4 – Wednesday and Thursday –** all even number posters will be on display.

*AWARD WINNERS

TECHNOLOGY

P-610

Comparison of Methanol, Acetonitrile and Ethanol with Respect to Solvent-Induced Artifact Formation in Biological Samples

PRESENTING AUTHOR: Bernhard Seidl, IFA-Tulln, University of Natural Resources and Life Sciences, Vienna (BOKU), Austria

CO-AUTHORS: Christoph Bueschl, Michael Sulyok, Rainer Schuhmacher

Organic solvents play a critical role in metabolomics experiments. They are usually indispensable for extraction or separation of metabolites from biological matrices. Ideally, the solvent should behave in an inert state and in no way alter the metabolites to be analyzed. While, it has already been shown that the extraction of plant material with methanol resulted in the formation of numerous solvent-induced artifacts (Sauerschnig et al., 2017), no studies on other common solvents have been published so far. Here, we have used a similar approach to investigate and compare artifact formation by acetonitrile, ethanol and methanol. To this end, the filamentous fungus Trichoderma reesei was cultivated in synthetic minimal liquid medium and both the culture media supernatant and the fungal mycelium were extracted separately with each of the three solvents. In each case, the native and deuterated form of the solvent was used in parallel. The experiment was also performed on fully labeled 13C samples to be able to unambiguously classify metabolites to be of biological origin and on a defined standard mix. The samples were measured by LC-HRMS and the respective measurement data was then searched for coeluting feature pairs with identical peak shape and whose m/z values differed by exactly a multiple of the mass increment between 1H and 2H. Similar to Sauerschnig et al. (2017), numerous artifacts induced by methanol were found. The poster will demonstrate that ethanol- and acetonitrile-derived extracts did also contain solvent-induced artifacts, albeit to a much lesser extent than in case of methanol.

P-611

Investigating metabolic flux in central metabolic pathways associated with isocitrate dehydrogenase 1 mutations using [1,2-13C2]Glucose

PRESENTING AUTHOR: Areesha Nazeer, University of Oxford, UK, United Kingdom

CO-AUTHORS: John Walsby-Tickle, Edward Smith, David Hauton, James S.O. McCullagh

Isocitrate dehydrogenase mutations are found in a range of cancers including glioma and amyloid leukaemia and produce a change of enzyme function leading to the production of significant amounts of 2-hydroxyglutarate (2-HG), a metabolite that has now become one of the best known small molecule biomarkers in cancer. The mechanism of tumorigenesis is still not fully understood however. A number of metabolomics studies have investigated changes in metabolite profiles but few have investigated the impact of the mutation on metabolic flux. In this study [1,2-13C2] glucose was used as a tracer to quantify metabolic flux in LN18 IDH mutant (IDH1) and IDH wild type cells. Anion-chromatography coupled to a Q-exactive orbitrap MS system was used to measure glycolytic, pentose phosphate pathway and TCA cycle metabolites and concomitant isotopomer distributions. INCA was then used to calculate flux values for each metabolic transformation by minimizing the difference between the measured labelling patterns and those simulated by the metabolic model with 95% confidence intervals. LN18 mutant cells show altered flux through central metabolic pathways, a high flux from α-ketoglutarate to 2-hydroxoglutarate with a decreased flux from α-ketoglutarate to succinate, fumarate and malate indicated decreased oxidation of glucose in the TCA cycle compared to LN18 wild type cells. A decreased flux through PDH and an increased flux from pyruvate to lactate through LDH suggested that IDH1 mutant cells undergo broader metabolic reprogramming than has previously been reported. It is anticipated these metabolic alterations in mutant IDH1 cells will help identify potential new therapeutic targets.

P-612

Tracer-based metabolomics for Immune Cells: Exploring Central Carbon Metabolism in Human Natural Killer Cells

PRESENTING AUTHOR: Doriane Lorendeau, Janssen R&D, Belgium

CO-AUTHORS: Liesbeth Vereyken, Ian Strickland, Rob J. Vreeken

While immunotherapy is emerging as a successful alternative approach to classical drug therapy for many disease areas, such as cancer and chronic infectious diseases, still little is known about the fine-tuning between metabolism and functions of immune cells in health and disease states. Notably, central carbon metabolism, as a central metabolic pathway providing energy, cellular building blocks and precursors for cell signaling and epigenetic factors, is of key interest to understand the major metabolic routes sustaining immune cells functions. To quantify central carbon metabolites and determine the relative contribution of specific metabolic pathways (i.e. intracellular metabolic fluxes), we established a tracer-based metabolomic platform using stable isotopes together with metabolic network modeling. At first, we established in vitro immune cell line models. Specifically, human lymphoma NK-92 and leukemia KHYG-1 natural killer cells were fed with 13C6-glucose for 24h, to reach isotopic steady state prior to metabolite extraction. To monitor the major branching routes of central carbon metabolism, including glycolysis, tricarboxylic acid cycle, pentose-phosphate pathways, we developed a general analytical method for small molecules-amino acids and -organic acids, using an UPLC HILIC column system for separation coupled with a Q-Exactive HF orbitrap mass spectrometer. First experiments demonstrated incorporation of the 13C isotope label into downstream metabolites of glucose. Next, this platform will be further validated by dissecting the various metabolic modes associated with different activation or inhibitory stimuli on NK cells in vitro and finally on (pre-)clinical samples of chronic infectious diseases (e.g. Hepatitis B, HIV...).
**POSTER SESSIONS 1 AND 2 – Monday and Tuesday –** all odd number posters will be on display.

**POSTER SESSIONS 3 AND 4 – Wednesday and Thursday –** all even number posters will be on display.

*AWARD WINNERS

TECHNOLOGY

P-613 Glutamine and nitrogen metabolism in cancer

PRESENTING AUTHOR: Susanne Fürst, BIMSB, MDC, DKFZ, DKTK, Germany

CO-AUTHORS: Safak Bayram, Guido Mastrobuoni, Christin Zasada, Stefan Kempa

Metabolic reprogramming is a required step during oncogenesis. It is triggered by activation of oncogenes and loss of tumor suppressors and leads to an activation of central metabolic pathways to support cell growth and proliferation. Already in the 1920th Otto Warburg identified glycolysis as deregulated in cancer. These findings highlighted central metabolism as therapeutic target. Beside the activation of the glycolytic pathway also nitrogen metabolism is enhanced in order to support the de-novo biosynthesis of nucleotides as building blocks of DNA and RNA synthesis. The required nitrogen can stem from amino acids like glutamine or alanine or from free ammonium. In order to quantify the usage and activity of metabolic pathways in vitro and in vivo we have developed pulsed stable isotope resolved metabolomics (pSIRM). The applied GC-MS based technology enables the absolute quantification of metabolites and at the same time the determination of stable isotope incorporation. In a next step to quantify the dynamics of nitrogen and carbon metabolism simultaneously we are developing new methods by applying ultra high resolution mass spectrometry. With the new technology we hope to better characterize the metabolic mode of cancer cells and to identify suitable therapeutic targets.

P-614 Evaluation of intestinal functionality using tracer metabolomics

PRESENTING AUTHOR: Bei-Tzu Wang, Drug Metabolism and PharmacoKinetics, Discovery Sciences, Janssen R&D, Belgium

CO-AUTHORS: Suzy Geerinckx, Liesbeth Vereyken, Kathleen Allaerts, Ronald De Vries, Filip Cuyckens, David Cassiman, Gianluca Matteoli, Bart Ghesquière, Rob J. Vreeken

The intestine is one of the key organs absorbing and metabolizing nutrients required for survival. Clinical phenotypes like e.g. inflammatory bowel disease, result in disturbed nutrient uptake and several health issues in patients. However, clinical tests used for monitoring intestinal functionality are either invasive, unspecific or unable to monitor the subtle changes. During inflammatory process, arachidonic acid (AA) is released through phospholipase A-2 mediated hydrolysis of epithelial phospholipids and further metabolized to prostaglandins and other eicosanoids. Therefore, we aim to establish a stable-isotope mediated metabolomics platform to monitor the function of intestine using stable isotope-labeled AA. For proof-of-concept purpose, we used Caco-2 cell model and induced its inflammatory response by interleukin-1β, tumor necrosis factor-α and interferon-γ. Furthermore, we feed Caco-2 cells with 13C-labeled AA and evaluate its incorporation into the phospholipids and its downstream metabolites. To monitor the changes of phospholipids, we have established a shotgun lipidomics platform, using nano-electrospray coupled to a Q-Exactive HF orbitrap mass spectrometer. To evaluate the release of eicosanoids, we used a targeted UPLC-MS/MS method (for 130 eicosanoids) on an API 6500 QTRAP system. Our first results confirmed uptake of C13-AA in the Caco-2 cells and release of prostaglandins into medium from cytokines-stimulated Caco-2 cells. These will also be further optimized in view of dynamics of inflammatory processes and further applied in various in-vivo systems.

P-615* Isotype-specific regulation of whole-body gluconeogenesis by AAK/AMPK in live C. elegans observed with real-time in-organism NMR metabolomics

PRESENTING AUTHOR: Tin Nguyen, Seoul National University, South Korea

CO-AUTHORS: Yong Jin An, Jin Wook Cha, Yoon-Joo Ko, Hanee Lee, Christine H Chung, Sang-Min Jeon, Junho Lee, Sunghyouk Park

Traditional metabolomics employs sample extraction of tissues and cells, making it difficult to understand the actual metabolic flux in a living system at the whole-organism level. Combining whole-body 13C-labeling strategy, NMR spectroscopy, and optimized sampling methods, we developed an in-organism NMR metabolomics approach for live C. elegans animals. The convenient approach allowed real-time monitoring of metabolic activity at the whole-body level of live worms with high sensitivity and resolution. When applied to C. elegans with mutations in AAK/AMPK, a metabolic master regulator, this approach revealed unique metabolic role of AAK-1 and AAK-2 isotypes in live C. elegans. Particularly, the aak-1 knockout animals exhibited enhanced glucose production under starvation, strikingly opposite to aak-2 knockout animals. Unusually high compensatory expression of the reciprocal isotypes in each mutant and the results for the double knockout suggested an unconventional phenotype-genotype relationship and the epistasis of aak-2 to aak-1 in glucose production. Mechanistically, the gene expression and 13C-isotope incorporation results showed that aak-1 KO metabolotypes are due to reduced TCA and glycolysis and enhanced gluconeogenesis through the activation of fatty acid oxidation and glyoxylate shunt. Revealing isotype-specific roles of the catalytic subunits of AAK/AMPK, our approach should be readily applicable to other metabolic models involving C. elegans.
POSTER SESSIONS 1 AND 2 – Monday and Tuesday – all odd number posters will be on display.

POSTER SESSIONS 3 AND 4 – Wednesday and Thursday – all even number posters will be on display.

*AWARD WINNERS

TECHNOLOGY

P-616 Wikidata and Scholia as a hub linking metabolite knowledge

PRESENTING AUTHOR: Egon Willighagen, Maastricht University, Netherlands

CO-AUTHORS: Denise Slenter, Daniel Mietchen, Chris T. Evelo, Finn Å. Nielsen

Making chemical databases more FAIR supports identification of unexpected chemical compounds (toxicants, drug metabolites, etc) in untargeted metabolomics experiments. We here discuss Wikidata: it is a machine readable database, boosting the interoperability of metabolite databases. Thanks to the Wikidata:WikiProject Chemistry community, there is a growing amount of information about metabolites: Wikidata currently has over 150 thousand compounds with InChIKeys and 70 thousand CAS numbers. Ongoing work by this WikiProject includes capturing chemical classes and chemical compounds in the various Wikipedias as machine readable data. Other projects include covering human drugs [2], MeSH Chemicals and Drugs, and volatile organic compounds. We here introduce our contributions to the WikiProject Chemistry to support FAIR-ification of metabolite knowledge. For example, we proposed Wikidata properties to annotate compounds with database identifiers for the EPA CompTox Dashboard [2], the SPLASH [3], and MassBank. Furthermore, we used a combination of Biocycle and QuickStatements to add missing compounds for biological pathways from WikiPathways [4]. Finally, we introduce an extension of Scholia [5], visualizing data about compounds and compound classes, including external identifiers, physicochemical properties, and an overview of the literature from which the knowledge is derived. At the time of writing, three thousand chemicals are linked to more than 170 thousand research articles. 2. Putman TE, et al. Database. 2017;2017(1). 3. Williams, AJ, et al. J. Cheminform. 2017;9:61. 4. Wohlgemuth G, et al. Nature Biotechnology. 2016;34(11):1099–101. 5. Slenter DN, et al. NAR. 2018;46:D661–D667. 6. Nielsen, FÅ, et al. The Semantic Web: ESWC 2017 Satellite Events, 2017.

P-617

Optimisation of nanoelectrospray direct infusion mass spectrometry metabolomics for 96-well based in vitro, high throughput screening

PRESENTING AUTHOR: Julia Malinowska, University of Birmingham, United Kingdom

CO-AUTHORS: Julia M. Malinowska, Taina Palosaari, Jukka Sund, Donatella Carpi, Maurice Whelan, Mark R. Viant

High throughput screening (HTS) is becoming a recognised approach for supporting decision-making in chemical safety assessment, whilst in vitro metabolomics is a promising tool to accelerate a departure from the use of animal models in toxicity testing. The objective of this study was to seek compatibility of high-resolution spectral-stitching nanoelectrospray direct infusion mass spectrometry (nanoDIMS) metabolomics (University of Birmingham) and an HTS platform (EU Reference Laboratory for Alternatives to Animal Testing). Low biomass cell samples (50,000 hepatocytes of HepaRG per well) were prepared for metabolomics analyses using a newly established automated protocol and solvent system (Biomek FXp laboratory automated workstation) and analysed using a modified nanoDIMS method (Thermo Scientific Orbitrap Elite) suitable for small sample sizes. The proposed method was assessed with respect to sensitivity and reproducibility of the entire workflow from HepaRG sampling to measurement of the metabolic phenotype. It has been demonstrated that the optimised nanoDIMS metabolomics method provides acceptable sensitivity (>3,500 non-background peaks routinely detected) as well as acceptable intra- and inter-plate variability (median relative standard deviation <30%) in both polar and nonpolar nanoDIMS assays. Baseline characterisation of HepaRG endogenous metabolism over time and a pilot study with a model toxicant (cadmium chloride) are underway, revealing changes in the metabolome through a 48-hr culture period. This newly established analytical method shows that in vitro metabolomics may be readily applied as an additional high content assay in 96-well HTS, complementing HT measures of the transcriptome and image phenotype.
<figure><img src="image_97.png" /></figure>

Author Index
<table><thead><tr><th>Name Last, First</th><th>Abstracts</th></tr></thead><tbody><tr><td colspan="2">* = Presenting Author</td></tr><tr><td>A, Middleton</td><td>P-179</td></tr><tr><td>Abda, Julia</td><td>P-575</td></tr><tr><td>Abdi, Fadi</td><td>P-600</td></tr><tr><td>Abdrabou, Wael</td><td>P-146*</td></tr><tr><td>Abe, Kaori</td><td>P-295</td></tr><tr><td>Abel, Ted</td><td>17D</td></tr><tr><td>Abnet, Christian</td><td>P-291</td></tr><tr><td>Abogye, E.</td><td>P-25</td></tr><tr><td>Abouleila, Yasmine</td><td>16C</td></tr><tr><td>Abraham, Neethu</td><td>P-201</td></tr><tr><td>Abreu, Ilka</td><td>2E</td></tr><tr><td>Achaintre, David</td><td>P-66</td></tr><tr><td>Achaintre, David</td><td>P-109</td></tr><tr><td>Acharjee, Animesh</td><td>P-171</td></tr><tr><td>Achkar, Iman</td><td>P-64</td></tr><tr><td>Adam, Tomáš</td><td>P-163</td></tr><tr><td>Adam, Tomáš</td><td>P-164</td></tr><tr><td>Adam, Tomáš</td><td>P-320</td></tr><tr><td>Adamski, Jerzy</td><td>P-111</td></tr><tr><td>Adamski, Jerzy</td><td>P-158</td></tr><tr><td>Adamski, Jerzy</td><td>P-169</td></tr><tr><td>Adamski, Jerzy</td><td>P-172</td></tr><tr><td>Adamski, Jerzy</td><td>P-188</td></tr><tr><td>Adamski, Jerzy</td><td>P-601</td></tr><tr><td>Adel-Patient, Karine</td><td>P-464</td></tr><tr><td>Adhami, Neema</td><td>P-324</td></tr><tr><td>Adhami, Neema</td><td>P-326</td></tr><tr><td>Afman, Lydia</td><td>6B</td></tr><tr><td>Agalou, M.</td><td>P-306</td></tr><tr><td>Agnieszka, Klupczynska</td><td>P-225</td></tr><tr><td>Agnieszka, Kowalewska</td><td>P-217</td></tr><tr><td>Agtuca, Beverly</td><td>16E</td></tr><tr><td>Aguilar, Esther</td><td>7A</td></tr><tr><td>Aharoni, Asaph</td><td>11B</td></tr><tr><td>Aharoni, Asaph</td><td>14B</td></tr><tr><td>Aharoni, Asaph</td><td>2C</td></tr><tr><td>Ahlborn, Natalie</td><td>P-117</td></tr><tr><td>Ahmad, Andrabi, Syed, Bilal</td><td>27C</td></tr><tr><td>Ahmed, Talaat</td><td>P-460</td></tr><tr><td>Ahn-Jarvis, Jennifer, H.</td><td>P-353</td></tr><tr><td>Ahola, Sofia</td><td>24B</td></tr><tr><td>Ahonen, Linda</td><td>P-150</td></tr><tr><td>Ahonen, Linda</td><td>P-174</td></tr><tr><td>Aida, Mrzic</td><td>11E</td></tr><tr><td>Aigner, Ludwig</td><td>9C</td></tr></tbody></table>

<table><thead><tr><th>Name Last, First</th><th>Abstracts</th></tr></thead><tbody><tr><td>Aimo, Lucila</td><td>19A</td></tr><tr><td>Akarasereenont, Pravit</td><td>P-456</td></tr><tr><td>Åkerfeldt, Torbjörn</td><td>P-93</td></tr><tr><td>Åkesson, A.</td><td>P-129</td></tr><tr><td>Åkesson, Agneta</td><td>P-115</td></tr><tr><td>Akkoyunlu, Mustafa</td><td>P-241</td></tr><tr><td>Aksenov, Alexander</td><td>P-602</td></tr><tr><td>Al, Wahsh, Mohammad</td><td>P-57</td></tr><tr><td>Alajmi, Mohamed</td><td>P-354*</td></tr><tr><td>Alamri, Hassan</td><td>P-79*</td></tr><tr><td>Alanazi, Samyah</td><td>P-134</td></tr><tr><td>Al-Baghdadi, Abdullah</td><td>23E</td></tr><tr><td>Albanes, Demetrius</td><td>P-39*</td></tr><tr><td>Albanes, Demetrius</td><td>P-24</td></tr><tr><td>Albergamo, Vittorio</td><td>P-511</td></tr><tr><td>Albóniga, Oihane, E.</td><td>P-244*</td></tr><tr><td>Albóniga, Oihane, E.</td><td>P-243</td></tr><tr><td>Alden</td><td>P-548</td></tr><tr><td>Alders, Mariëlle</td><td>P-167</td></tr><tr><td>Aleksandra, Szybiak</td><td>P-217</td></tr><tr><td>Alenezi, Samya</td><td>P-134</td></tr><tr><td>Alenzi, Naif, D.</td><td>P-134</td></tr><tr><td>Alexander-Hardiman, Kate</td><td>10C</td></tr><tr><td>Alexandrov, Theodore</td><td>12B</td></tr><tr><td>Alexandrov, Theodore</td><td>16A*</td></tr><tr><td>Ali, Ahmed</td><td>16C*</td></tr><tr><td>Alinaghi, Masoumeh</td><td>P-488*</td></tr><tr><td>Allaerts, Kathleen</td><td>P-614</td></tr><tr><td>Allard, Pierre-Marie</td><td>11B</td></tr><tr><td>Al-Majdoub, Mahmoud</td><td>P-176</td></tr><tr><td>Almvik, Marit</td><td>P-434</td></tr><tr><td>Alonso, David, E.</td><td>P-30</td></tr><tr><td>Alonso, Rosa, M.</td><td>P-243</td></tr><tr><td>Alonso, Rosa, M.</td><td>P-244</td></tr><tr><td>Alourfi, Nouf</td><td>P-524*</td></tr><tr><td>Alqarni, Abdulmalik</td><td>P-134</td></tr><tr><td>Alshehry, Zahir</td><td>13B</td></tr><tr><td>Altenbuchinger, Michael</td><td>P-228</td></tr><tr><td>Altendorfer-Kroath, Thomas</td><td>P-185</td></tr><tr><td>Álvarez-Muñoz, Diana</td><td>P-302*</td></tr><tr><td>Alves, Leonardo, Jadyr, Silva, Rodrigues</td><td>P-82</td></tr><tr><td>AlWahsh, Mohammad</td><td>P-269*</td></tr><tr><td>Alzamora, Rosa</td><td>P-459</td></tr></tbody></table>

<table><thead><tr><th>Name Last, First</th><th>Abstracts</th></tr></thead><tbody><tr><td>Alzamora, Rosa</td><td>P-463</td></tr><tr><td>Amante, Eleonora</td><td>P-21*</td></tr><tr><td>Amar, Laurence</td><td>P-80</td></tr><tr><td>Amiano, Pilar</td><td>P-227</td></tr><tr><td>Ammerlaan, Brenda</td><td>P-516*</td></tr><tr><td>Amster, I., Jonathan</td><td>P-260</td></tr><tr><td>An, Yanpeng</td><td>P-161</td></tr><tr><td>An, Yong, Jin</td><td>P-615</td></tr><tr><td>Anand, Sonia</td><td>P-125</td></tr><tr><td>Anand, Sonia</td><td>P-121</td></tr><tr><td>Andalete, Lourdes, Urban</td><td>P-365</td></tr><tr><td>Andersen, Erik</td><td>26A</td></tr><tr><td>Andersen, Maria, K.</td><td>P-67*</td></tr><tr><td>Anderson, Dovile</td><td>P-140*</td></tr><tr><td>Anderton, Christopher</td><td>16E</td></tr><tr><td>Anderton, Christopher, R.</td><td>P-261</td></tr><tr><td>André, Valérie</td><td>P-201</td></tr><tr><td>Andreassen, Trygve</td><td>P-221</td></tr><tr><td>Andres-Lacueva, Cristina</td><td>P-351*</td></tr><tr><td>Andrés-Lacueva, Cristina</td><td>6C</td></tr><tr><td>Andrés-Lacueva, Cristina</td><td>9C</td></tr><tr><td>Ankley, Gerald</td><td>P-315</td></tr><tr><td>Anstee, Quentin</td><td>P-157</td></tr><tr><td>Antibas, Atik, Fernando</td><td>P-82</td></tr><tr><td>Antonelli, Michela</td><td>P-547</td></tr><tr><td>António, Carla</td><td>P-406</td></tr><tr><td>Antunes, Elisa, Ribeiro</td><td>P-417</td></tr><tr><td>Aoki, Yuichi</td><td>P-105</td></tr><tr><td>Aoki, Yutaka</td><td>P-178</td></tr><tr><td>Aono, Yusuke</td><td>P-430*</td></tr><tr><td>Apffel, Alex</td><td>P-293</td></tr><tr><td>Appleby, Paul, N.</td><td>P-109</td></tr><tr><td>Ara, Takeshi</td><td>19B</td></tr><tr><td>Ara, Takeshi</td><td>P-356</td></tr><tr><td>Ara, Takeshi</td><td>P-366</td></tr><tr><td>Arakawa, Kiyomi</td><td>P-594</td></tr><tr><td>Araújo, Ana, Margarida</td><td>P-272*</td></tr><tr><td>Ardanaz, Eva</td><td>P-227</td></tr><tr><td>Arita, Masanori</td><td>15B</td></tr><tr><td>Arita, Masanori</td><td>19B</td></tr><tr><td>Arita, Masanori</td><td>30A</td></tr><tr><td>Arita, Masanori</td><td>P-229</td></tr><tr><td>Armenta, Danny</td><td>P-172</td></tr></tbody></table>

<table><thead><tr><th>Name Last, First</th><th>Abstracts</th></tr></thead><tbody><tr><td>Armitage, Emily</td><td>P-110*</td></tr><tr><td>Armstrong, Michael, D.S.</td><td>P-582</td></tr><tr><td>Arnold, Jonathan</td><td>P-264</td></tr><tr><td>Arnold, Rebecca, S.</td><td>P-26</td></tr><tr><td>Arnold, Stefan</td><td>16B</td></tr><tr><td>Arora, Manish</td><td>P-28</td></tr><tr><td>Arreondo-Campos, O., Rene</td><td>P-582</td></tr><tr><td>Arslan, Deniz</td><td>P-15</td></tr><tr><td>Artati, Anna</td><td>P-172*</td></tr><tr><td>Arts, Ilja</td><td>5B*</td></tr><tr><td>Aru, Violetta</td><td>15E</td></tr><tr><td>Aru, Violetta</td><td>P-395*</td></tr><tr><td>Arumugam, Krithika</td><td>10D</td></tr><tr><td>Arvidsson, Torbjörn</td><td>P-71</td></tr><tr><td>Asami, Mariko</td><td>P-178</td></tr><tr><td>Ashley, Dean</td><td>P-179*</td></tr><tr><td>Asikin, Yonathan</td><td>P-430</td></tr><tr><td>Assunção, Nilson</td><td>P-154*</td></tr><tr><td>Astley, Christina</td><td>5D</td></tr><tr><td>Astrup, Arne</td><td>5B</td></tr><tr><td>Atauri, Pedro</td><td>7A</td></tr><tr><td>Audran, Michel</td><td>24C</td></tr><tr><td>Augustin, Megan</td><td>11B</td></tr><tr><td>Aura, Anna-Marja</td><td>P-382</td></tr><tr><td>Auray-Blais, Christiane</td><td>P-190*</td></tr><tr><td>Auray-Blais, Christiane</td><td>P-13</td></tr><tr><td>Auriola, Seppo</td><td>P-382</td></tr><tr><td>Auriola, Seppo</td><td>P-536</td></tr><tr><td>Aust, Anne-Christine</td><td>P-213</td></tr><tr><td>Authier, Simon</td><td>P-220</td></tr><tr><td>Avila-Pacheco, Julian</td><td>28A</td></tr><tr><td>Axelsen, Kristian</td><td>19A</td></tr><tr><td>Azab, Sandi</td><td>P-125*</td></tr><tr><td>Azab, Sandi</td><td>P-121</td></tr><tr><td>Azevedo, Ana, Isabel</td><td>P-23</td></tr><tr><td>Azizi, Michel</td><td>P-80</td></tr><tr><td>Badia, Míriam</td><td>P-56</td></tr><tr><td>Badosa, Esther</td><td>P-318</td></tr><tr><td>Baghel, Ruchi</td><td>P-540*</td></tr><tr><td>Bai, Lulu</td><td>22E</td></tr><tr><td>Baierl, Andreas</td><td>P-66</td></tr><tr><td>Bajzikova, Martina</td><td>P-53</td></tr><tr><td>Baker, Kristie</td><td>P-585</td></tr><tr><td>Baker, Paul, RS</td><td>P-587</td></tr><tr><td>Bakke, James</td><td>P-220</td></tr></tbody></table>
<table><thead><tr><th>Name Last, First</th><th>Abstracts</th></tr></thead><tbody><tr><td colspan="2">* = Presenting Author</td></tr><tr><td>Bakker, Barbara</td><td>P-210</td></tr><tr><td>Bakker, Noëlle</td><td>P-268</td></tr><tr><td>Balasubramanian, Raji</td><td>P-128</td></tr><tr><td>Balcells, Cristina</td><td>7A</td></tr><tr><td>Balcke, Gerd</td><td>11B</td></tr><tr><td>Balcke, Gerd</td><td>23B*</td></tr><tr><td>Baldi, Pierre</td><td>P-172</td></tr><tr><td>Balgoma, David</td><td>P-187*</td></tr><tr><td>Ballet, Caroline</td><td>4B</td></tr><tr><td>Balluff, Benjamin</td><td>P-67</td></tr><tr><td>Bally, Lia</td><td>P-568</td></tr><tr><td>Balogh, Andras</td><td>P-202</td></tr><tr><td>Balvay, Aurélie</td><td>28B</td></tr><tr><td>Bamba, Takeshi</td><td>P-578*</td></tr><tr><td>Ban, Jae-Suk</td><td>P-411</td></tr><tr><td>Bandukwala, Abbas</td><td>P-600</td></tr><tr><td>Banerjee, Rintu</td><td>25B</td></tr><tr><td>Banerji, U.</td><td>P-25</td></tr><tr><td>Banka, Siddharth</td><td>P-167</td></tr><tr><td>Bansal, Parit</td><td>19A</td></tr><tr><td>Baptist, Haley</td><td>1D</td></tr><tr><td>Baquer, Gerard</td><td>P-519</td></tr><tr><td>Baraldi, Eugenio</td><td>P-247</td></tr><tr><td>Barbas, Coral</td><td>21C</td></tr><tr><td>Barbas, Coral</td><td>P-32</td></tr><tr><td>Barbas, Coral</td><td>P-75</td></tr><tr><td>Barbas, Coral</td><td>P-82</td></tr><tr><td>Barbas, Coral</td><td>P-85</td></tr><tr><td>Barbas, Coral</td><td>P-92</td></tr><tr><td>Barbas, Coral</td><td>P-227</td></tr><tr><td>Barbera, Giorgia, La</td><td>P-547*</td></tr><tr><td>Barco, Sebastiano</td><td>P-527</td></tr><tr><td>Baricchi, Roberto</td><td>P-81</td></tr><tr><td>Baricchi, Roberto</td><td>P-226</td></tr><tr><td>Barlow, Christopher</td><td>13B</td></tr><tr><td>Barman, Malin</td><td>P-235</td></tr><tr><td>Barnes, Elizabeth</td><td>13B</td></tr><tr><td>Barran, Perdita</td><td>17B</td></tr><tr><td>Barretto, Sharon</td><td>P-160*</td></tr><tr><td>Barrio, Ramón, J.</td><td>P-242</td></tr><tr><td>Barron, Leon, P.</td><td>P-311</td></tr><tr><td>Barsch, Aiko</td><td>2B</td></tr><tr><td>Barsch, Aiko</td><td>4E</td></tr><tr><td>Barsch, Aiko</td><td>P-196</td></tr><tr><td>Barsch, Aiko</td><td>P-266</td></tr><tr><td>Barsch, Aiko</td><td>P-298</td></tr><tr><td>Barsch, Aiko</td><td>P-508</td></tr><tr><td>Barsch, Aiko</td><td>P-590</td></tr></tbody></table>

<table><thead><tr><th>Name Last, First</th><th>Abstracts</th></tr></thead><tbody><tr><td colspan="2">* = Presenting Author</td></tr><tr><td>Barsch, Aiko</td><td>P-600</td></tr><tr><td>Bartolomaeus, Hendrik</td><td>P-202</td></tr><tr><td>Bartsch, Cornelia</td><td>P-86</td></tr><tr><td>Barua, Meenakshi</td><td>P-54*</td></tr><tr><td>Barupal, Dinesh, Kumar</td><td>P-257</td></tr><tr><td>Baskaran, Kumaran</td><td>P-494</td></tr><tr><td>Bateman, Lucinda</td><td>P-119</td></tr><tr><td>Bathe, Oliver, F.</td><td>P-27</td></tr><tr><td>Bathe, Ulschan</td><td>11B</td></tr><tr><td>Bathen, Tone, F.</td><td>P-20</td></tr><tr><td>Bathen, Tone, F.</td><td>P-47</td></tr><tr><td>Bathen, Tone, F.</td><td>P-67</td></tr><tr><td>Bathen, Tone, F.</td><td>P-221</td></tr><tr><td>Bathen, Tone, F.</td><td>P-270</td></tr><tr><td>Batolucci, Martina</td><td>P-527</td></tr><tr><td>Battelini, Furio</td><td>P-350</td></tr><tr><td>Batut, Aurélie</td><td>P-577</td></tr><tr><td>Bauer, Anna</td><td>P-552*</td></tr><tr><td>Baur, Joseph</td><td>17D</td></tr><tr><td>Bayne, Haley</td><td>P-9*</td></tr><tr><td>Bayram, Safak</td><td>P-613</td></tr><tr><td>Bazanella, Monika</td><td>18E</td></tr><tr><td>BD, McNally</td><td>P-179</td></tr><tr><td>Beale, Michael</td><td>29C</td></tr><tr><td>Beale, Michael, H.</td><td>P-413</td></tr><tr><td>Bearden, Dan</td><td>P-600</td></tr><tr><td>Beaumont, Martin</td><td>6B</td></tr><tr><td>Beuseroy, Pierre</td><td>P-48</td></tr><tr><td>Beavis, Guy</td><td>P-398</td></tr><tr><td>Becker, Claude</td><td>P-455</td></tr><tr><td>Becker, Michael</td><td>P-294</td></tr><tr><td>Beckmann, M.</td><td>P-329</td></tr><tr><td>Beckmann, Manfred</td><td>14D</td></tr><tr><td>Beckmann, Manfred</td><td>P-343</td></tr><tr><td>Bedia, Carmen</td><td>P-56*</td></tr><tr><td>Bednarek, Paweł</td><td>P-454</td></tr><tr><td>Beecher, Chris</td><td>P-530*</td></tr><tr><td>Beecher, Chris</td><td>P-565</td></tr><tr><td>Beecher, Chris</td><td>P-487</td></tr><tr><td>Beecher, Chris</td><td>P-288</td></tr><tr><td>Beekman, Marian</td><td>P-118*</td></tr><tr><td>Beekman, Marian</td><td>P-84</td></tr><tr><td>Beekwilder, Jules</td><td>14B</td></tr><tr><td>Beger, Richard</td><td>P-241*</td></tr><tr><td>Beger, Richard</td><td>P-600</td></tr><tr><td>Behrends, Volker</td><td>21B*</td></tr></tbody></table>

<table><thead><tr><th>Name Last, First</th><th>Abstracts</th></tr></thead><tbody><tr><td colspan="2">* = Presenting Author</td></tr><tr><td>Behrens, Diana</td><td>P-33</td></tr><tr><td>Beis, D.</td><td>P-306</td></tr><tr><td>Bekhti, Nihel</td><td>P-464*</td></tr><tr><td>Belharazem, Djeda</td><td>P-269</td></tr><tr><td>Belharazem, Djeda</td><td>P-57</td></tr><tr><td>Bell, Jimmy</td><td>P-181</td></tr><tr><td>Bellaire, Anke</td><td>P-448</td></tr><tr><td>Bellisai, Alice</td><td>P-413*</td></tr><tr><td>Bellvert, Floriant</td><td>P-38*</td></tr><tr><td>Bellvert, Floriant</td><td>P-38</td></tr><tr><td>Belzung, Catherine</td><td>P-234</td></tr><tr><td>Bendiks, Zachary</td><td>P-191</td></tr><tr><td>Bene, Nicolas</td><td>P-201</td></tr><tr><td>Benítez, Alfonso</td><td>6B</td></tr><tr><td>Benito, Sandra</td><td>P-242*</td></tr><tr><td>Benjamen, Maillard</td><td>P-325</td></tr><tr><td>Benke, Peter, I.</td><td>P-305</td></tr><tr><td>Benkovič, Samuel</td><td>P-476</td></tr><tr><td>Bennouna, Djawed</td><td>P-34</td></tr><tr><td>Bentlage, Marco</td><td>P-436</td></tr><tr><td>Berchtold, Christian</td><td>16B*</td></tr><tr><td>Berden, Giel</td><td>11A</td></tr><tr><td>Berden, Giel</td><td>P-265</td></tr><tr><td>Berden, Giel</td><td>P-534</td></tr><tr><td>Berden, Giel</td><td>P-576</td></tr><tr><td>Bergamini, Stefania</td><td>P-81</td></tr><tr><td>Bergareche, Alberto</td><td>P-227</td></tr><tr><td>Bergdahl, Ingvar</td><td>P-129</td></tr><tr><td>Bergdahl, Ingvar</td><td>P-115</td></tr><tr><td>Bergdahl, Ingvar</td><td>P-116</td></tr><tr><td>Bergen, Martin</td><td>P-317</td></tr><tr><td>Berggren, Johan</td><td>P-176</td></tr><tr><td>Bergmann, Sven</td><td>P-470*</td></tr><tr><td>Bergmann, Sven</td><td>P-255</td></tr><tr><td>Bergmann, Sven</td><td>P-545</td></tr><tr><td>Bergsten, Peter</td><td>P-188</td></tr><tr><td>Bergström, Ed</td><td>P-406</td></tr><tr><td>Bergthaler, Andreas</td><td>21D</td></tr><tr><td>Berjanskii, Mark</td><td>P-400</td></tr><tr><td>Berkers, Celia</td><td>1D</td></tr><tr><td>Bernaerts, K</td><td>12E</td></tr><tr><td>Bernick, Kristin, B.</td><td>P-293</td></tr><tr><td>Bernier, Michel</td><td>P-29</td></tr><tr><td>Bernillon, Stephane</td><td>P-422</td></tr><tr><td>Berntsen, Hanne, Friis</td><td>P-301</td></tr><tr><td>Berntsen, Hanne, Friis</td><td>P-314</td></tr><tr><td>Berrios-Carcamo, Pablo</td><td>P-108</td></tr></tbody></table>

<table><thead><tr><th>Name Last, First</th><th>Abstracts</th></tr></thead><tbody><tr><td colspan="2">* = Presenting Author</td></tr><tr><td>Bertelkamp, Cheryl</td><td>P-304</td></tr><tr><td>Bertherat, Jérôme</td><td>P-80</td></tr><tr><td>Bertilsson, Helena</td><td>P-20</td></tr><tr><td>Bertilsson, Helena</td><td>P-67</td></tr><tr><td>Bertram, Hanne, Christine</td><td>P-488</td></tr><tr><td>Bertrand, Cédric</td><td>P-303*</td></tr><tr><td>Bertrand, Cédric</td><td>P-318</td></tr><tr><td>Bertrand-Michel, Justine</td><td>P-577*</td></tr><tr><td>Bessarab, Irina</td><td>10D</td></tr><tr><td>Bethan, Bianca</td><td>P-600</td></tr><tr><td>Bétoulières, Colette</td><td>P-160</td></tr><tr><td>Beule, Dieter</td><td>P-483</td></tr><tr><td>Beule, Dieter</td><td>P-549</td></tr><tr><td>Beuschlein, Felix</td><td>P-80</td></tr><tr><td>Bhatia, Anil</td><td>2B</td></tr><tr><td>Bhattacharyya, Parthasarathi</td><td>25B</td></tr><tr><td>Bhattacharyya, Parthasarathi</td><td>P-253</td></tr><tr><td>Bhot, Meeta</td><td>P-54</td></tr><tr><td>Bianchi, Flavia</td><td>P-462*</td></tr><tr><td>Biancolillo, Alessandra</td><td>P-21</td></tr><tr><td>Bichet, Daniel</td><td>P-190</td></tr><tr><td>Bieлеń, Agata</td><td>P-19</td></tr><tr><td>Billon, Yvon</td><td>P-238</td></tr><tr><td>Binkley, Joe</td><td>P-30</td></tr><tr><td>Birer, Caroline</td><td>P-261*</td></tr><tr><td>Biringer, Roger</td><td>P-554</td></tr><tr><td>Birner-Gruenberger, Ruth</td><td>P-52</td></tr><tr><td>Birngruber, Thomas</td><td>P-185</td></tr><tr><td>Bizec, Bruno</td><td>P-549</td></tr><tr><td>Blaak, Ellen</td><td>5B</td></tr><tr><td>Blachier, François</td><td>6B</td></tr><tr><td>Blanc, Eric</td><td>P-549</td></tr><tr><td>Blanch, Joan, Miró</td><td>28B*</td></tr><tr><td>Blanco, David, Rojo</td><td>P-32</td></tr><tr><td>Blanco, María, E.</td><td>P-244</td></tr><tr><td>Blank, Lars, M.</td><td>P-372</td></tr><tr><td>Bliziotis, Nikolaos</td><td>P-80*</td></tr><tr><td>Block, Anna</td><td>P-438</td></tr><tr><td>Blom, Henk</td><td>P-182</td></tr><tr><td>Bloomfield, Jonathon</td><td>P-121</td></tr><tr><td>Blum, David, L.</td><td>P-390</td></tr><tr><td>Blume, Martin</td><td>P-142</td></tr><tr><td>Boccard, Julien</td><td>P-535</td></tr><tr><td>Böcker, Sebastian</td><td>P-539</td></tr></tbody></table>
<table><thead><tr><th>Name Last, First</th><th>Abstracts</th></tr></thead><tbody><tr><td colspan="2">* = Presenting Author</td></tr><tr><td>Bocobza, Samuel</td><td>14B</td></tr><tr><td>Bodai, Zsolt</td><td>28A</td></tr><tr><td>Bodard, Sylvie</td><td>P-87</td></tr><tr><td>Bodin, Johanna</td><td>P-301</td></tr><tr><td>Bodin, Johanna</td><td>P-314</td></tr><tr><td>Boer, Siebolt</td><td>P-265</td></tr><tr><td>Boer, Theo</td><td>P-210</td></tr><tr><td>Boerjan, Wout</td><td>P-424</td></tr><tr><td>Boerjan, Wout</td><td>P-431</td></tr><tr><td>Boerjan, Wout</td><td>P-556</td></tr><tr><td>Boers, Hanny, M.</td><td>P-338</td></tr><tr><td>Bofin, Anna, M.</td><td>P-20</td></tr><tr><td>Boguszewicz, Łukasz</td><td>P-19*</td></tr><tr><td>Bolleman, Jerven</td><td>19A</td></tr><tr><td>Bollen, Maike</td><td>P-349*</td></tr><tr><td>Bongaerts, Michiel</td><td>P-182*</td></tr><tr><td>Bonini, Paolo</td><td>P-257*</td></tr><tr><td>Bonte, Ramon</td><td>P-182</td></tr><tr><td>Borchers, Christoph, H.</td><td>P-537</td></tr><tr><td>Bordag, Natalie</td><td>23E*</td></tr><tr><td>Borengasser, Sarah, J.</td><td>P-122</td></tr><tr><td>Borewicz, Klaudyna</td><td>P-382</td></tr><tr><td>Borgsmüller, Nico</td><td>P-549</td></tr><tr><td>Borkum, Mark</td><td>P-557</td></tr><tr><td>Borodulin, Katja</td><td>P-120</td></tr><tr><td>Boscaro, Marco</td><td>P-80</td></tr><tr><td>Bosch, Annet, M.</td><td>P-180</td></tr><tr><td>Bouchemal, Nadia</td><td>P-193</td></tr><tr><td>Boughton, Berin, A.</td><td>P-258</td></tr><tr><td>Boukalova, Stepana</td><td>P-53</td></tr><tr><td>Boulangé, Claire</td><td>9B</td></tr><tr><td>Bours, Martijn</td><td>P-66</td></tr><tr><td>Boutin, Michel</td><td>P-13*</td></tr><tr><td>Boutin, Michel</td><td>P-190</td></tr><tr><td>Bouwman, Jildau</td><td>6B*</td></tr><tr><td>Bouwmeester, Harro</td><td>P-436</td></tr><tr><td>Bouwmeester, Harro</td><td>P-442</td></tr><tr><td>Bovet, Cédric</td><td>P-560*</td></tr><tr><td>Bovet, Cédric</td><td>P-568</td></tr><tr><td>Bovy, Arnaud</td><td>14B</td></tr><tr><td>Bowden, Donald</td><td>9B</td></tr><tr><td>Bowen, Christopher</td><td>P-579</td></tr><tr><td>Boyce, Mary</td><td>P-207*</td></tr><tr><td>Boyce, Mary</td><td>P-282</td></tr><tr><td>Boyce, Mary</td><td>P-290</td></tr><tr><td>Boyd, Mark</td><td>P-31</td></tr><tr><td>Brack, Werner</td><td>P-490</td></tr></tbody></table>

<table><thead><tr><th>Name Last, First</th><th>Abstracts</th></tr></thead><tbody><tr><td colspan="2">* = Presenting Author</td></tr><tr><td>Brack, Werner</td><td>P-512</td></tr><tr><td>Brady, Siobhan</td><td>P-442</td></tr><tr><td>Brandenburg, Marci</td><td>15D</td></tr><tr><td>Brandmaier, Stefan</td><td>P-188</td></tr><tr><td>Brandolini, Marion</td><td>P-155</td></tr><tr><td>Brann, John</td><td>P-277</td></tr><tr><td>Brantner, Christine</td><td>16E</td></tr><tr><td>Braun, Lukas, M.</td><td>P-50</td></tr><tr><td>Braun, Thomas</td><td>16B</td></tr><tr><td>Bravo-Santano, Natalia</td><td>21B</td></tr><tr><td>Brazdova, Lada</td><td>P-374*</td></tr><tr><td>Brcic, Luka</td><td>P-52</td></tr><tr><td>Bredvik, Kirsten</td><td>P-89</td></tr><tr><td>Brejnrod, Asker</td><td>15C</td></tr><tr><td>Brennan, Lorraine</td><td>18C</td></tr><tr><td>Brennan, Lorraine</td><td>P-339</td></tr><tr><td>Brennan, Lorraine</td><td>P-358</td></tr><tr><td>Brenner, Gábor</td><td>P-91</td></tr><tr><td>Brezina, Stefanie</td><td>P-66*</td></tr><tr><td>Brezina, Stefanie</td><td>P-66</td></tr><tr><td>Brezinova, Marie</td><td>P-8</td></tr><tr><td>Bridge, Alan</td><td>19A*</td></tr><tr><td>Bridge, Alan</td><td>P-535</td></tr><tr><td>Britz-McKibbin, Philip</td><td>P-121*</td></tr><tr><td>Britz-McKibbin, Philip</td><td>P-125</td></tr><tr><td>Britz-McKibbin, Philip</td><td>P-537</td></tr><tr><td>Bro, Rasmus</td><td>P-21</td></tr><tr><td>Broadhurst, David</td><td>11B</td></tr><tr><td>Broadhurst, David</td><td>15A*</td></tr><tr><td>Broadhurst, David</td><td>P-207</td></tr><tr><td>Broadhurst, David</td><td>P-256</td></tr><tr><td>Broadhurst, David</td><td>P-282</td></tr><tr><td>Broadhurst, David</td><td>P-600</td></tr><tr><td>Brock, Olaf</td><td>P-511</td></tr><tr><td>Broeckling, Corey</td><td>11B</td></tr><tr><td>Broer, Stefan</td><td>P-189</td></tr><tr><td>Broggini, Massimo</td><td>P-36</td></tr><tr><td>Broniowska, Kasia</td><td>P-46</td></tr><tr><td>Broström, Eva, Berggren</td><td>P-251</td></tr><tr><td>Brouckaert, Marlies</td><td>P-424*</td></tr><tr><td>Brouwers, Jos</td><td>10A*</td></tr><tr><td>Brown, William, B.</td><td>P-449</td></tr><tr><td>Brua, Robert</td><td>P-300*</td></tr><tr><td>Bruel, Sandrine</td><td>P-160</td></tr><tr><td>Bruelheide, Helge</td><td>P-451</td></tr><tr><td>Bruhn, Laurakay</td><td>P-293</td></tr></tbody></table>

<table><thead><tr><th>Name Last, First</th><th>Abstracts</th></tr></thead><tbody><tr><td colspan="2">* = Presenting Author</td></tr><tr><td>Brunelli, Laura</td><td>P-36*</td></tr><tr><td>Brunelli, Laura</td><td>P-3</td></tr><tr><td>Brunius, Carl</td><td>P-116*</td></tr><tr><td>Brunius, Carl</td><td>P-129</td></tr><tr><td>Brunius, Carl</td><td>P-115</td></tr><tr><td>Brunius, Carl</td><td>P-235</td></tr><tr><td>Brunius, Carl</td><td>P-497</td></tr><tr><td>Brunk, Elizabeth</td><td>P-386</td></tr><tr><td>Brunner, Andrea, Mizzi</td><td>P-304*</td></tr><tr><td>Brunner, Andreas-David</td><td>4E</td></tr><tr><td>Bruno, Lapauw</td><td>P-156</td></tr><tr><td>Bruno, Patris</td><td>P-325</td></tr><tr><td>Brasadelli, Marion</td><td>12D</td></tr><tr><td>Buchacher, Tanja</td><td>27C</td></tr><tr><td>Büchel, Felix</td><td>P-420</td></tr><tr><td>Budzinski, Ilara</td><td>2E*</td></tr><tr><td>Buescher, Joerg</td><td>11B</td></tr><tr><td>Bueschl, Christoph</td><td>P-609*</td></tr><tr><td>Bueschl, Christoph</td><td>P-440</td></tr><tr><td>Bueschl, Christoph</td><td>P-455</td></tr><tr><td>Bueschl, Christoph</td><td>P-610</td></tr><tr><td>Buisson, Corinne</td><td>24C</td></tr><tr><td>Bujold, Kim</td><td>P-220</td></tr><tr><td>Bull, Caroline</td><td>P-173*</td></tr><tr><td>Bunch, Josephine</td><td>P-59</td></tr><tr><td>Buratti, Martin</td><td>P-206</td></tr><tr><td>Buratti, Martin</td><td>P-573</td></tr><tr><td>Burgering, Boudewijn, M.T.</td><td>P-270</td></tr><tr><td>Burgos, Rosilene, Cristina, Rosetto</td><td>P-604</td></tr><tr><td>Burke, Adam</td><td>10C</td></tr><tr><td>Burman, Joachim</td><td>P-93</td></tr><tr><td>Burman, Joachim</td><td>P-197</td></tr><tr><td>Burton, Lyle</td><td>P-551</td></tr><tr><td>Bury, Nicolas, R.</td><td>P-311</td></tr><tr><td>Butkiewicz, Dorota</td><td>P-19</td></tr><tr><td>Butsugan, Michio</td><td>P-578</td></tr><tr><td>Buzkova, Jana</td><td>24B</td></tr><tr><td>Bystrický, Peter</td><td>P-65*</td></tr><tr><td>C, Manach</td><td>P-542</td></tr><tr><td>Cahoreau, Edern</td><td>P-160</td></tr><tr><td>Cai, Yuheng</td><td>P-559</td></tr><tr><td>Cai, Yuping</td><td>P-72*</td></tr><tr><td>Caiola, Elisa</td><td>P-36</td></tr><tr><td>Cajka, Tomas</td><td>P-8</td></tr><tr><td>Cajka, Tomas</td><td>P-79</td></tr></tbody></table>

<table><thead><tr><th>Name Last, First</th><th>Abstracts</th></tr></thead><tbody><tr><td colspan="2">* = Presenting Author</td></tr><tr><td>Calder, Elizabeth, L.</td><td>P-89</td></tr><tr><td>Calle, Yolanda</td><td>21B</td></tr><tr><td>CAM, Bink, Marco</td><td>2D</td></tr><tr><td>Cambiaghi, Alice</td><td>P-3</td></tr><tr><td>Cameron, Hunter</td><td>P-201</td></tr><tr><td>Cameron, Simon</td><td>10C</td></tr><tr><td>Cameron, Simon</td><td>7D</td></tr><tr><td>Cameron, Simon</td><td>P-517</td></tr><tr><td>Cameron, Simon, J.</td><td>P-575</td></tr><tr><td>Campbell, Natalie</td><td>P-125</td></tr><tr><td>Campbell, Peter, T.</td><td>P-102</td></tr><tr><td>Campbell, Wayne, W.</td><td>P-122</td></tr><tr><td>Campiglia, Pietro</td><td>P-274</td></tr><tr><td>Campisi, Judith</td><td>P-10</td></tr><tr><td>Campos, Jasna, Valentina</td><td>P-459*</td></tr><tr><td>Campos, Valentina</td><td>P-463</td></tr><tr><td>Camuzeaux, Stephane</td><td>P-149</td></tr><tr><td>Canario, Laurianne</td><td>P-238</td></tr><tr><td>Cangemi, Giuliana</td><td>P-527</td></tr><tr><td>Canlet, Cecile</td><td>P-160</td></tr><tr><td>Canlet, Cécile</td><td>P-155</td></tr><tr><td>Canlet, Cécile</td><td>P-238</td></tr><tr><td>Canlet, Cécile</td><td>P-541</td></tr><tr><td>Cannistraci, Carlo, V</td><td>P-120</td></tr><tr><td>Cao, Peijian</td><td>P-421</td></tr><tr><td>Capellades, Jordi</td><td>28B</td></tr><tr><td>Capriotti, Anna, Laura</td><td>P-547</td></tr><tr><td>Cardenas, Pablo</td><td>14B</td></tr><tr><td>Carlsson, Cecilia</td><td>P-94</td></tr><tr><td>Carlsson, Cecilia</td><td>P-157</td></tr><tr><td>Carlsson, Cecilia</td><td>P-301</td></tr><tr><td>Carlsson, Henrik</td><td>P-28</td></tr><tr><td>Carmeliet, G.</td><td>12E</td></tr><tr><td>Carpi, Donatella</td><td>P-617</td></tr><tr><td>Carrato, Alfredo</td><td>P-60</td></tr><tr><td>Carroll, Christopher</td><td>24B</td></tr><tr><td>Carter, Brian, D.</td><td>P-102</td></tr><tr><td>Carvalho, Agostinho</td><td>21C</td></tr><tr><td>Carvalho, Félix</td><td>P-272</td></tr><tr><td>Carvalho, Luciani, Renata, Silveira</td><td>P-154</td></tr><tr><td>Carvalho, Márcia</td><td>P-23</td></tr><tr><td>Carvalho, Márcia</td><td>P-272</td></tr><tr><td>Carvalho, Teresa</td><td>7B</td></tr><tr><td>Carvalho, Valdemir, Melechco</td><td>P-604</td></tr><tr><td>Cascante, Marta</td><td>3B</td></tr></tbody></table>
<table><thead><tr><th>Name Last, First</th><th>Abstracts</th></tr></thead><tbody><tr><td colspan="2">* = Presenting Author</td></tr><tr><td>Cascante, Marta</td><td>7A*</td></tr><tr><td>Cassan, Cedric</td><td>P-422</td></tr><tr><td>Cassan, Cédric</td><td>P-444</td></tr><tr><td>Cassiman, David</td><td>P-614</td></tr><tr><td>Castano-Cerezo, Sara</td><td>P-38</td></tr><tr><td>Castelli, Florence</td><td>P-136*</td></tr><tr><td>Castelli, Florence</td><td>P-155</td></tr><tr><td>Castelli, Florence</td><td>P-464</td></tr><tr><td>Castelnau, Pierre</td><td>P-87</td></tr><tr><td>Castro-Alves, Victor</td><td>P-278*</td></tr><tr><td>Cataldi, Thaís, Regiani</td><td>P-429</td></tr><tr><td>Caudy, Amy, A.</td><td>P-393</td></tr><tr><td>Cavaliere, Chiara</td><td>P-547</td></tr><tr><td>Cavallin, Jenna</td><td>P-315</td></tr><tr><td>Ceccato, Filippo</td><td>P-80</td></tr><tr><td>Cech, Nadja, B.</td><td>P-503</td></tr><tr><td>Cecil, Alexander</td><td>P-111</td></tr><tr><td>Ceglia, Nicholas, J.</td><td>P-172</td></tr><tr><td>Celine, Lukowicz</td><td>P-325</td></tr><tr><td>Centelles, Josep</td><td>7A</td></tr><tr><td>Centeno, Delphine</td><td>9C</td></tr><tr><td>Center, Sharon, A.</td><td>P-135</td></tr><tr><td>Ceramic, Asja</td><td>P-609</td></tr><tr><td>Cernada, María</td><td>P-203</td></tr><tr><td>Cerrato, Andrea</td><td>P-547</td></tr><tr><td>Cha, Jaeho</td><td>P-333</td></tr><tr><td>Cha, Jin, Wook</td><td>P-615</td></tr><tr><td>Chae, SiHyun</td><td>P-137*</td></tr><tr><td>Chae, Woori</td><td>P-224*</td></tr><tr><td>Chakravadhanula, Lalithasushma</td><td>P-482*</td></tr><tr><td>Chakravadhanula, Lalithasushma</td><td>P-268</td></tr><tr><td>Chakravarty, Baidyanath</td><td>P-237</td></tr><tr><td>Chaleckis, Romanas</td><td>30A</td></tr><tr><td>Chaleckis, Romanas</td><td>P-250*</td></tr><tr><td>Chaleckis, Romanas</td><td>P-251</td></tr><tr><td>Chalmers, John</td><td>13B</td></tr><tr><td>Chalupsky, Karel</td><td>P-518</td></tr><tr><td>Chamarro, Jennifer, Modamio</td><td>27B</td></tr><tr><td>Chamberlain, Casey</td><td>P-211*</td></tr><tr><td>Chamberlain, Casey</td><td>P-438</td></tr><tr><td>Chambers, Edward, S.</td><td>P-343</td></tr><tr><td>Chambers, John</td><td>P-188</td></tr><tr><td>Chan, Andrew, T.</td><td>P-22</td></tr><tr><td>Chan, Chi-On</td><td>P-184</td></tr><tr><td>CHAN, Chi-On</td><td>P-360</td></tr></tbody></table>

<table><thead><tr><th>Name Last, First</th><th>Abstracts</th></tr></thead><tbody><tr><td colspan="2">* = Presenting Author</td></tr><tr><td>Chan, Queenie</td><td>P-248</td></tr><tr><td>Chan, Shun, Wan</td><td>P-184*</td></tr><tr><td>Chan, Wendy</td><td>P-201</td></tr><tr><td>Chang, Ancheol</td><td>P-407</td></tr><tr><td>Chang, Chung-ke</td><td>P-99*</td></tr><tr><td>Chang, Jiakang</td><td>P-596</td></tr><tr><td>Chang, Polly</td><td>P-220</td></tr><tr><td>Chao, His-Chun</td><td>13E</td></tr><tr><td>Chao, His-Chun</td><td>P-96</td></tr><tr><td>Chao, Yen-Ming</td><td>P-88</td></tr><tr><td>Chapman, Elinor</td><td>P-31*</td></tr><tr><td>Charkoftaki, Georgia</td><td>P-108*</td></tr><tr><td>Charlton, Adrian</td><td>P-398</td></tr><tr><td>Chatsiricharoenkul, Somruedee</td><td>P-456</td></tr><tr><td>Chaudhury, Koel</td><td>25B</td></tr><tr><td>Chaudhury, Koel</td><td>P-237*</td></tr><tr><td>Chaudhury, Koel</td><td>P-253</td></tr><tr><td>Chaumont, Martin</td><td>P-252</td></tr><tr><td>Cheema, Amrita</td><td>P-608</td></tr><tr><td>Chekmeneva, Elena</td><td>P-248</td></tr><tr><td>Chekmeneva, Elena</td><td>P-475</td></tr><tr><td>Chen, Chang</td><td>22E*</td></tr><tr><td>Chen, Chih-Cheng</td><td>P-88</td></tr><tr><td>Chen, Kai-Ling</td><td>P-502</td></tr><tr><td>Chen, Kang</td><td>6E*</td></tr><tr><td>Chen, Lianmin</td><td>28C*</td></tr><tr><td>Chen, Qiansi</td><td>P-421</td></tr><tr><td>Chen, Qiansi</td><td>P-423</td></tr><tr><td>Chen, Qiuying</td><td>1B</td></tr><tr><td>Chen, Qiuying</td><td>P-89*</td></tr><tr><td>Chen, Siyuan</td><td>P-359</td></tr><tr><td>Chen, Songjie</td><td>P-130*</td></tr><tr><td>Chen, Ting-Zhen</td><td>P-309*</td></tr><tr><td>Chen, Wang</td><td>P-416</td></tr><tr><td>Chen, Wen-Ling</td><td>P-309</td></tr><tr><td>Chen, Xiong</td><td>P-561</td></tr><tr><td>Chen, Yi-Ting</td><td>P-595</td></tr><tr><td>Chenard-Poirier, M.</td><td>P-25</td></tr><tr><td>Cheng, Roger, R.</td><td>P-89</td></tr><tr><td>Cheng, Tsun-Jen</td><td>P-309</td></tr><tr><td>Cheong, Yu, Eun</td><td>P-377*</td></tr><tr><td>Chesler, Louis</td><td>1C</td></tr><tr><td>Chevanne, Chloé</td><td>P-444</td></tr><tr><td>Chiang, Meng-Han</td><td>P-236*</td></tr><tr><td>Chikwati, Elvis</td><td>P-395</td></tr><tr><td>Chim, Yuen-Ting</td><td>P-575</td></tr><tr><td>Chin, Hong, Miko, Poh</td><td>29A</td></tr></tbody></table>

<table><thead><tr><th>Name Last, First</th><th>Abstracts</th></tr></thead><tbody><tr><td colspan="2">* = Presenting Author</td></tr><tr><td>Chiu, Chih-Yung</td><td>P-236</td></tr><tr><td>Cho, Byung-Kwan</td><td>P-380</td></tr><tr><td>Cho, Joo-Youn</td><td>P-216</td></tr><tr><td>Cho, Joo-Youn</td><td>P-224</td></tr><tr><td>Cho, Joo-Youn</td><td>P-233</td></tr><tr><td>Cho, Joo-Youn</td><td>P-137</td></tr><tr><td>Cho, Kyong, Jin</td><td>P-144</td></tr><tr><td>Cho, Seungil</td><td>P-101</td></tr><tr><td>Cho, Su-yeon</td><td>P-341</td></tr><tr><td>Chocholoušková, Michaela</td><td>13A</td></tr><tr><td>Choe, Jeong, Sook</td><td>P-341</td></tr><tr><td>Choi, A, Young</td><td>P-319</td></tr><tr><td>Choi, Bo-Ram</td><td>P-411</td></tr><tr><td>Choi, Mira</td><td>P-380*</td></tr><tr><td>Choi, Mira</td><td>P-319</td></tr><tr><td>Choi, Tudor, Jennifer</td><td>17D</td></tr><tr><td>Choi, Yongsoo</td><td>P-363</td></tr><tr><td>Chong, Jasmine</td><td>P-498*</td></tr><tr><td>Choo, Kenny</td><td>P-349</td></tr><tr><td>Choudhury, Priyanka</td><td>25B</td></tr><tr><td>Choudhury, Priyanka</td><td>P-253*</td></tr><tr><td>Chouinard, Christopher</td><td>P-585*</td></tr><tr><td>Chowdhury, Debjani, Bhattacharyya</td><td>P-400</td></tr><tr><td>Chowdhury, Sushmita, Roy</td><td>25B</td></tr><tr><td>Chowdhury, Sushmita, Roy</td><td>P-253</td></tr><tr><td>Christensen, Hanne, Bjerre</td><td>P-580</td></tr><tr><td>Christensen, Shawn</td><td>P-438*</td></tr><tr><td>Christiansen, Nina</td><td>P-174</td></tr><tr><td>Christoph, Turck</td><td>P-205</td></tr><tr><td>Christopherson, Claus, T</td><td>P-207</td></tr><tr><td>Chu, Rosalie, K.</td><td>P-261</td></tr><tr><td>Chu, Su</td><td>25A</td></tr><tr><td>Chu, Su</td><td>25C</td></tr><tr><td>Chu, Su</td><td>P-477*</td></tr><tr><td>Chu, Yajing</td><td>P-171</td></tr><tr><td>Chuang, Derrick</td><td>P-373</td></tr><tr><td>Chung, Bong, Chui</td><td>P-194</td></tr><tr><td>Chung, Christine, H</td><td>P-615</td></tr><tr><td>Chung, Wonil</td><td>P-7</td></tr><tr><td>Church, Deirdre</td><td>P-141</td></tr><tr><td>Chu-Van, Emeline</td><td>P-136</td></tr><tr><td>Ciborowski, Michal</td><td>P-40*</td></tr><tr><td>Ciociola, Tecla</td><td>P-387</td></tr></tbody></table>

<table><thead><tr><th>Name Last, First</th><th>Abstracts</th></tr></thead><tbody><tr><td colspan="2">* = Presenting Author</td></tr><tr><td>Ciszek, Mateusz</td><td>P-19</td></tr><tr><td>Ciurli, Alessio</td><td>P-271*</td></tr><tr><td>Claes, Britt</td><td>P-67</td></tr><tr><td>Clark, Adam</td><td>P-107</td></tr><tr><td>Clark, Christopher</td><td>P-14</td></tr><tr><td>Claus, Sandrine</td><td>6B</td></tr><tr><td>Clayton, Peter, E.</td><td>P-167</td></tr><tr><td>Clement, Fiona</td><td>P-141</td></tr><tr><td>Clendinen, Chaevien, S.</td><td>P-26</td></tr><tr><td>Clerens, Stefan</td><td>18A</td></tr><tr><td>Clish, Clary</td><td>P-480</td></tr><tr><td>Clish, Clary</td><td>P-477</td></tr><tr><td>Clish, Clary</td><td>P-600</td></tr><tr><td>Cobankovic, Iva</td><td>P-91*</td></tr><tr><td>Cobankovic, Iva</td><td>P-91</td></tr><tr><td>Cochrane, Keeva</td><td>26B</td></tr><tr><td>Cochrane, Keeva</td><td>3B</td></tr><tr><td>Cochrane, Keeva</td><td>P-596</td></tr><tr><td>Codesido, Santiago</td><td>P-535*</td></tr><tr><td>Coelho, Ana, R.</td><td>P-53</td></tr><tr><td>Coene, Karlien</td><td>P-265*</td></tr><tr><td>Coene, Karlien</td><td>P-576</td></tr><tr><td>Colby, Sean</td><td>30B</td></tr><tr><td>Colby, Sean</td><td>P-297</td></tr><tr><td>Colby, Sean</td><td>P-557</td></tr><tr><td>Cole, Joanne</td><td>5D</td></tr><tr><td>Collado, María, Carmen</td><td>P-203</td></tr><tr><td>Collado, María, Carmen</td><td>P-347</td></tr><tr><td>Collette, Tim</td><td>P-315</td></tr><tr><td>Collins, Anne</td><td>P-579</td></tr><tr><td>Colsch, Benoit</td><td>P-155</td></tr><tr><td>Comte, Blandine</td><td>P-155</td></tr><tr><td>Comte, Blandine</td><td>P-501</td></tr><tr><td>Concepcion, Crystal</td><td>P-365</td></tr><tr><td>Conlon, Mike</td><td>P-296*</td></tr><tr><td>Connell, JM</td><td>P-80</td></tr><tr><td>Conti, Stefania</td><td>P-387</td></tr><tr><td>Conway, Louis</td><td>4B</td></tr><tr><td>Cooperstone, Jessica</td><td>P-353*</td></tr><tr><td>Coorey, Ranil</td><td>P-349</td></tr><tr><td>Coppes, Wouter</td><td>P-592</td></tr><tr><td>Corbin, Agnes</td><td>P-608*</td></tr><tr><td>Corbin, Laura</td><td>P-114</td></tr><tr><td>Corbin, Laura</td><td>P-173</td></tr><tr><td>Cornett, Shannon</td><td>P-294</td></tr></tbody></table>
<table><thead><tr><th>Name Last, First</th><th>Abstracts</th></tr></thead><tbody><tr><td colspan="2">* = Presenting Author</td></tr><tr><td>Correia, Evelyne</td><td>P-136</td></tr><tr><td>Correia, Goncalo</td><td>P-59</td></tr><tr><td>Correia, Mário</td><td>4B</td></tr><tr><td>Correig, Xavier</td><td>P-60</td></tr><tr><td>Correig, Xavier</td><td>P-324</td></tr><tr><td>Correig, Xavier</td><td>P-326</td></tr><tr><td>Correig, Xavier</td><td>P-519</td></tr><tr><td>Cort, John</td><td>P-297</td></tr><tr><td>Cort, John</td><td>P-557</td></tr><tr><td>Costenbader, Karen</td><td>P-477</td></tr><tr><td>Côté, Anne-Marie</td><td>P-190</td></tr><tr><td>Coudert, Elisabeth</td><td>19A</td></tr><tr><td>Coulibaly, Aboubacar, S.</td><td>P-146</td></tr><tr><td>Coulier, Leon</td><td>P-592*</td></tr><tr><td>Coulier, Leon</td><td>P-516</td></tr><tr><td>Cousins, Ben</td><td>17C</td></tr><tr><td>Cox, Eric</td><td>P-138</td></tr><tr><td>Coyle, Seamus</td><td>P-31</td></tr><tr><td>Crawford, Elizabeth</td><td>P-588*</td></tr><tr><td>Creek, Darren</td><td>7B*</td></tr><tr><td>Creek, Darren, J.</td><td>P-140</td></tr><tr><td>Cross, Justin, R.</td><td>P-586</td></tr><tr><td>Crouzillat, Dominique</td><td>2D</td></tr><tr><td>Cruciani, Gabriele</td><td>P-547</td></tr><tr><td>Cruickshank-Quinn, Charmion</td><td>22D</td></tr><tr><td>Cruickshank-Quinn, Charmion</td><td>P-11*</td></tr><tr><td>Cseh, Edina</td><td>P-90</td></tr><tr><td>Cui, Jing</td><td>P-418*</td></tr><tr><td>Cui, Jing</td><td>P-477</td></tr><tr><td>Culp, Joseph, M.</td><td>P-300</td></tr><tr><td>Cumeras, Raquel</td><td>P-60*</td></tr><tr><td>Cunha, Cristina</td><td>21C</td></tr><tr><td>Cuparencu, Cătălina, S.</td><td>P-332</td></tr><tr><td>Curtasu, Mihai, Victor</td><td>P-191*</td></tr><tr><td>Cuvertino, Sara</td><td>P-167</td></tr><tr><td>Cuyckens, Filip</td><td>P-148</td></tr><tr><td>Cuyckens, Filip</td><td>P-534</td></tr><tr><td>Cuyckens, Filip</td><td>P-570</td></tr><tr><td>Cuyckens, Filip</td><td>P-614</td></tr><tr><td>Czajkowski, Marcin</td><td>21C</td></tr><tr><td>D, Low</td><td>P-542</td></tr><tr><td>D., O.</td><td>P-232</td></tr><tr><td>D'Alessandro, Angelo</td><td>P-279</td></tr><tr><td>D'Alessandro, Angelo</td><td>P-285</td></tr></tbody></table>

<table><thead><tr><th>Name Last, First</th><th>Abstracts</th></tr></thead><tbody><tr><td colspan="2">* = Presenting Author</td></tr><tr><td>da, Cruz, Pedro, Luis, Rocha</td><td>P-604</td></tr><tr><td>da, Silva, Hana, Karina, Pereira</td><td>P-429*</td></tr><tr><td>da, Silva, Lucas, H.F.</td><td>22B</td></tr><tr><td>da, Silva, Ricardo</td><td>P-544</td></tr><tr><td>da, Silva, Ricardo</td><td>P-602</td></tr><tr><td>da, Silva, Ricardo, R.</td><td>15C</td></tr><tr><td>Dadova, Klara</td><td>P-8</td></tr><tr><td>Dai, Yuqin</td><td>P-574*</td></tr><tr><td>Dai, Yuqin</td><td>P-130</td></tr><tr><td>Dai, Yuqin</td><td>P-586</td></tr><tr><td>Dalloux-Chioccioli, Jessica</td><td>P-577</td></tr><tr><td>Daly, Ronan</td><td>P-510</td></tr><tr><td>Damalas, Dimitrios</td><td>P-306*</td></tr><tr><td>Daniel, Hannelore</td><td>P-104</td></tr><tr><td>Daniel, Hannelore</td><td>P-112</td></tr><tr><td>Daniel, Hannelore</td><td>P-168</td></tr><tr><td>Daniel, Hannelore</td><td>P-181</td></tr><tr><td>Dannhorn, Andreas</td><td>P-59</td></tr><tr><td>das, Dores, Amanda, V.B.</td><td>22B</td></tr><tr><td>Dasari, Surendara</td><td>P-600</td></tr><tr><td>Dashti, Hesam</td><td>P-494</td></tr><tr><td>Dauly, Claire</td><td>P-434</td></tr><tr><td>Dauwe, Rebecca</td><td>29B*</td></tr><tr><td>Dauwe, Rebecca</td><td>P-431</td></tr><tr><td>Dauwe, Rebecca</td><td>P-556</td></tr><tr><td>Davanture, Marlène</td><td>P-418</td></tr><tr><td>David, Herzog</td><td>P-205</td></tr><tr><td>Davies, Alun</td><td>13C</td></tr><tr><td>Davies, Eleanor</td><td>P-80</td></tr><tr><td>Davies, Vinny</td><td>P-510</td></tr><tr><td>Davis, Clay</td><td>P-599</td></tr><tr><td>Davison, Andrew</td><td>5C</td></tr><tr><td>Day, Philip</td><td>16D</td></tr><tr><td>Dayal, Hiranya</td><td>P-322*</td></tr><tr><td>Dayalan, Saravanan</td><td>3B</td></tr><tr><td>Daygon, Venea, Dara</td><td>P-453*</td></tr><tr><td>Daygon, Venea, Dara</td><td>P-365</td></tr><tr><td>de, Boer, H.C.</td><td>12C</td></tr><tr><td>de, Geus-Oei, Lioe-Fee</td><td>P-118</td></tr><tr><td>de, Grooth, Harm-Jan</td><td>1D</td></tr><tr><td>De, Henauw, Stefaan</td><td>P-170</td></tr><tr><td>de, Jong, F</td><td>P-565</td></tr><tr><td>de, Jong, Felice</td><td>P-487*</td></tr><tr><td>de, Jong, Felice</td><td>P-530</td></tr></tbody></table>

<table><thead><tr><th>Name Last, First</th><th>Abstracts</th></tr></thead><tbody><tr><td colspan="2">* = Presenting Author</td></tr><tr><td>de, la, Fuente, Irene, Romero</td><td>14B</td></tr><tr><td>De, la, Torre, Capilla</td><td>P-42</td></tr><tr><td>de, Lourdes, Bastos, Maria</td><td>P-23</td></tr><tr><td>de, Lourdes, Bastos, Maria</td><td>P-272</td></tr><tr><td>de, Mas, Igor, marin</td><td>7A</td></tr><tr><td>de, Moura, Leite, Fernando, Brunale, Vilela</td><td>P-78</td></tr><tr><td>de, Olivera, Eliandre</td><td>P-3</td></tr><tr><td>De, Paepe, Ellen</td><td>P-138</td></tr><tr><td>De, Paoli, Amanda</td><td>P-140</td></tr><tr><td>de, Pinho, Paula, Guedes</td><td>P-23</td></tr><tr><td>de, Pinho, Paula, Guedes</td><td>P-272</td></tr><tr><td>de, Rooji, Susanne</td><td>P-12</td></tr><tr><td>de, Souza, AdrianaCarvalho</td><td>P-371</td></tr><tr><td>de, Souza, AdrianaCarvalho</td><td>P-516</td></tr><tr><td>de, Souza, David</td><td>P-579</td></tr><tr><td>de, Souza, Russell, J.</td><td>P-121</td></tr><tr><td>De, Spiegeleer, Margot</td><td>P-98*</td></tr><tr><td>de, Tullio, Pascal</td><td>P-15*</td></tr><tr><td>de, Voogt, Pim</td><td>P-511</td></tr><tr><td>De, Vos, Ric</td><td>11B</td></tr><tr><td>de, Vos, Ric</td><td>14A*</td></tr><tr><td>de, Vos, Ric</td><td>P-457</td></tr><tr><td>de, Vos, Ric</td><td>P-292</td></tr><tr><td>de, Vos, Ric, CH</td><td>2D</td></tr><tr><td>De, Vries, Ronald</td><td>P-614</td></tr><tr><td>Debik, Julia</td><td>P-47*</td></tr><tr><td>Debik, Julia</td><td>P-221</td></tr><tr><td>Deborde, Catherine</td><td>P-396</td></tr><tr><td>Debrah, Alex</td><td>P-148</td></tr><tr><td>Debrah, Linda, Batsa</td><td>P-148</td></tr><tr><td>Deda, Olga</td><td>P-110</td></tr><tr><td>Deelen, Joris</td><td>P-118</td></tr><tr><td>Dehghan, Abbas</td><td>9B</td></tr><tr><td>Dehghan, Abbas</td><td>P-475</td></tr><tr><td>Deinum, Jaap</td><td>P-80</td></tr><tr><td>Del, Borrello, Samantha</td><td>P-393</td></tr><tr><td>Delorenzi, Mauro</td><td>P-14</td></tr><tr><td>delPalacio, José, Pérez</td><td>P-42</td></tr><tr><td>Delvaux, Aurélie</td><td>P-136</td></tr></tbody></table>

<table><thead><tr><th>Name Last, First</th><th>Abstracts</th></tr></thead><tbody><tr><td colspan="2">* = Presenting Author</td></tr><tr><td>Demeestere, Kristof</td><td>P-307</td></tr><tr><td>Demianova, Zuzana</td><td>P-607</td></tr><tr><td>Demirdas, Serwet</td><td>P-182</td></tr><tr><td>Denery, Judith</td><td>P-375</td></tr><tr><td>Denman, Sandra</td><td>14D</td></tr><tr><td>Dennedy, Michael, Conall</td><td>P-80</td></tr><tr><td>Derkach, Andriy</td><td>P-24</td></tr><tr><td>Derr, Leslie</td><td>P-600</td></tr><tr><td>Dervilly, Gaud</td><td>24C</td></tr><tr><td>Dervilly-Pinel, G.</td><td>P-571</td></tr><tr><td>Desai, Dipika</td><td>P-121</td></tr><tr><td>Desmet, Sandrien</td><td>P-431*</td></tr><tr><td>Desmet, Sandrien</td><td>P-556</td></tr><tr><td>Desnouveaux, Laura</td><td>P-145</td></tr><tr><td>Desoubzdanne, Denis</td><td>P-145*</td></tr><tr><td>DeSouza, Russell</td><td>P-125</td></tr><tr><td>Dethloff, Frederik</td><td>P-205*</td></tr><tr><td>DeVries, A., Courtney</td><td>P-34</td></tr><tr><td>Dhandapani, Savitha</td><td>P-426</td></tr><tr><td>Dhungana, Suraj</td><td>P-600</td></tr><tr><td>di, Bartolomeo, Erminia</td><td>P-81</td></tr><tr><td>Di, Giovanni, Nicolas</td><td>P-55*</td></tr><tr><td>Di, Poto, Cristina</td><td>P-30</td></tr><tr><td>Di, Xinyu</td><td>P-569*</td></tr><tr><td>Diana, Álvarez-Muñoz</td><td>P-302</td></tr><tr><td>Diana, P.</td><td>P-529</td></tr><tr><td>Diarra, Amidou</td><td>P-146</td></tr><tr><td>Diawara, Aïssatou</td><td>P-146</td></tr><tr><td>Diaz, Heli</td><td>18D</td></tr><tr><td>Diaz, Heli</td><td>P-352</td></tr><tr><td>Díaz-Beltrán, Leticia</td><td>P-42</td></tr><tr><td>Diaz-Fernandez, Paloma</td><td>P-575</td></tr><tr><td>Díaz Navarro, Caridad</td><td>P-42</td></tr><tr><td>Diaz-Rubio, M., Elena</td><td>P-135*</td></tr><tr><td>Dib, Shaima</td><td>P-64</td></tr><tr><td>Dibbets-Schneider, Petra</td><td>P-118</td></tr><tr><td>Dickens, Alex</td><td>27C</td></tr><tr><td>Dickens, Alex</td><td>P-95*</td></tr><tr><td>Dickens, Alex</td><td>P-94</td></tr><tr><td>Dickinson, Elizabeth</td><td>P-398*</td></tr><tr><td>Dieng, Mame, Massar</td><td>P-146</td></tr><tr><td>Diez, Simon, Carmen</td><td>P-357*</td></tr><tr><td>Dillen, Lieve</td><td>P-148</td></tr><tr><td>Dillen, Lieve</td><td>P-570</td></tr><tr><td>Ding, Caroline</td><td>P-410</td></tr></tbody></table>
<table><thead><tr><th>Name Last, First</th><th>Abstracts</th></tr></thead><tbody><tr><td colspan="2">* = Presenting Author</td></tr><tr><td>Dingemans, Milou</td><td>P-304</td></tr><tr><td>Dirk, Valkenborg</td><td>11E</td></tr><tr><td>Dirven, Hubert</td><td>P-301</td></tr><tr><td>Dirven, Hubert</td><td>P-314</td></tr><tr><td>Doell, Stefanie</td><td>11B</td></tr><tr><td>Doenges, Katrina, A.</td><td>P-122</td></tr><tr><td>Doerig, Christian</td><td>7B</td></tr><tr><td>Dolan, Kathleen</td><td>P-393</td></tr><tr><td>Dólleman, S.C.</td><td>12C</td></tr><tr><td>Dominik, Rachoń</td><td>P-217</td></tr><tr><td>Donarski, James</td><td>P-398</td></tr><tr><td>Donati, Stefano</td><td>3D</td></tr><tr><td>Donat-Vargas, Carolina</td><td>P-129</td></tr><tr><td>Donat-Vargas, Carolina</td><td>P-115</td></tr><tr><td>Dong, Ying</td><td>P-400</td></tr><tr><td>Doppler, Maria</td><td>P-440*</td></tr><tr><td>Doppler, Maria</td><td>P-455</td></tr><tr><td>Doppler, Maria</td><td>P-609</td></tr><tr><td>Dormoi, Jérôme</td><td>P-145</td></tr><tr><td>Dorrestein, Pieter</td><td>15C</td></tr><tr><td>Dorrestein, Pieter</td><td>28A</td></tr><tr><td>Dorrestein, Pieter, C.</td><td>P-384</td></tr><tr><td>Dorrestein, Pieter, C.</td><td>P-544</td></tr><tr><td>Dorrestein, Pieter, C.</td><td>P-602</td></tr><tr><td>dos, Santos, Fabio, Neves</td><td>P-82</td></tr><tr><td>Dossin, E.</td><td>P-529</td></tr><tr><td>Dossin, E.</td><td>P-565</td></tr><tr><td>Dragsted, Lars, Ove</td><td>P-174</td></tr><tr><td>Dragsted, Lars, Ove</td><td>P-332</td></tr><tr><td>Drake, Richard, R.</td><td>P-294</td></tr><tr><td>Draper, J.</td><td>P-329</td></tr><tr><td>Draper, John</td><td>14D</td></tr><tr><td>Draper, John</td><td>P-343</td></tr><tr><td>Drautz-Moses, Daniela</td><td>10D</td></tr><tr><td>Dreher, Patrick</td><td>P-112*</td></tr><tr><td>du, Chatinier, Wilma</td><td>18B</td></tr><tr><td>Dubbelman, Anne-Charlotte</td><td>P-570*</td></tr><tr><td>Dubbelman, Anne-Charlotte</td><td>P-84</td></tr><tr><td>Duberg, Daniel</td><td>P-301</td></tr><tr><td>Duberg, Daniel</td><td>P-314</td></tr><tr><td>Dubery, Ian</td><td>14C</td></tr><tr><td>Dubery, Ian</td><td>P-439*</td></tr><tr><td>Dubery, Ian</td><td>P-447</td></tr><tr><td>Duchateau, Lucien</td><td>P-385</td></tr></tbody></table>

<table><thead><tr><th>Name Last, First</th><th>Abstracts</th></tr></thead><tbody><tr><td>Duchoslav, Eva</td><td>P-473</td></tr><tr><td>Dudek, Christian-Alexander</td><td>P-338</td></tr><tr><td>Dudoit, Sandrine</td><td>P-28</td></tr><tr><td>Dudzik, Danuta</td><td>P-29*</td></tr><tr><td>Dührkop, Kai</td><td>P-539</td></tr><tr><td>Dunn, Warwick</td><td>P-107*</td></tr><tr><td>Dunn, Warwick</td><td>P-581</td></tr><tr><td>Dunn, Warwick</td><td>P-603</td></tr><tr><td>Dunn, Warwick</td><td>P-600</td></tr><tr><td>Dupuy, Camille</td><td>P-87</td></tr><tr><td>Durand, Stephanie</td><td>9C</td></tr><tr><td>Durand, Stéphanie</td><td>P-549</td></tr><tr><td>Duren, William</td><td>15D</td></tr><tr><td>Duren, William</td><td>P-495</td></tr><tr><td>Dwivany, Fenny, M.</td><td>P-428</td></tr><tr><td>Dyar, Kenneth, A.</td><td>P-172</td></tr><tr><td>Dykes, Gary</td><td>P-349</td></tr><tr><td>Dyrlund, Thomas, Sparholt</td><td>P-150</td></tr><tr><td>e, Paiva, Caria, Cíntia, Rabelo</td><td>P-388</td></tr><tr><td>Earl, Ashlee</td><td>P-141</td></tr><tr><td>Earnest, Kaylin</td><td>12D</td></tr><tr><td>Ebbels, Timothy</td><td>19E*</td></tr><tr><td>Ebbels, Timothy</td><td>9B</td></tr><tr><td>Ebbels, Timothy, M.D.</td><td>P-124</td></tr><tr><td>Ebbels, Timothy, M.D.</td><td>P-248</td></tr><tr><td>Ebbels, Timothy, M.D.</td><td>P-475</td></tr><tr><td>Ebbels, Timothy, M.D.</td><td>P-559</td></tr><tr><td>Ebbels, Timothy, M.D.</td><td>P-600</td></tr><tr><td>Eberlin, Marcos, Nogueira</td><td>P-82</td></tr><tr><td>Eberlin, Marcos, Nogueira</td><td>P-417</td></tr><tr><td>EC, Considine</td><td>P-525</td></tr><tr><td>Eckel-Mahan, Kristin</td><td>P-172</td></tr><tr><td>Ecker, Josef</td><td>P-168</td></tr><tr><td>Ediage, Emmanuel, Njumbe</td><td>P-148</td></tr><tr><td>Edison, Arthur</td><td>26A*</td></tr><tr><td>Edison, Arthur, S.</td><td>P-26</td></tr><tr><td>Edison, Arthur, S.</td><td>P-260</td></tr><tr><td>Edison, Arthur, S.</td><td>P-264</td></tr><tr><td>Edison, Arthur, S.</td><td>P-390</td></tr><tr><td>Edison, Arthur, S.</td><td>P-485</td></tr><tr><td>Edmands, William, M.B.</td><td>P-28</td></tr><tr><td>Edouard, Louis</td><td>P-55</td></tr><tr><td>Egert, B.</td><td>P-409</td></tr></tbody></table>

<table><thead><tr><th>Name Last, First</th><th>Abstracts</th></tr></thead><tbody><tr><td>Eghbalnia, Hamid, R.</td><td>P-494</td></tr><tr><td>Ehrhardt, Thomas</td><td>P-327</td></tr><tr><td>Ehrsson, Ylva, Tiblom</td><td>P-71</td></tr><tr><td>Eisenberger, Alina</td><td>P-483</td></tr><tr><td>Eisenhofer, Graeme</td><td>P-80</td></tr><tr><td>Eisenstecken, Daniela</td><td>P-462</td></tr><tr><td>Ekasari, Julie</td><td>P-361</td></tr><tr><td>Ekman, Drew</td><td>P-315</td></tr><tr><td>El, Assal, Diana</td><td>17C</td></tr><tr><td>Elbadawi-Sidhu, Mona</td><td>P-375*</td></tr><tr><td>Eleftherakos, Karolos</td><td>P-281</td></tr><tr><td>Elemento, Olivier</td><td>P-64</td></tr><tr><td>Elfrink, Hyung, L.</td><td>P-167</td></tr><tr><td>Eliassen, A., Heather</td><td>P-22</td></tr><tr><td>Eliassen, A., Heather</td><td>P-480</td></tr><tr><td>Elicone, Christopher</td><td>P-277</td></tr><tr><td>Elkins, Aaron</td><td>22A</td></tr><tr><td>Ellero-Simatos, Sandrine</td><td>P-160</td></tr><tr><td>Ellershaw, John</td><td>P-31</td></tr><tr><td>Elliott, Paul</td><td>9B</td></tr><tr><td>Elliott, Paul</td><td>P-475</td></tr><tr><td>Ellis, James</td><td>21B</td></tr><tr><td>Ellisor, Deb</td><td>P-599</td></tr><tr><td>Emilien, Jamin</td><td>P-325</td></tr><tr><td>Emmanuel, Elijah</td><td>P-205</td></tr><tr><td>Emmerich, Michael</td><td>P-482</td></tr><tr><td>Emond, Patrick</td><td>P-87*</td></tr><tr><td>Emond, Patrick</td><td>P-234</td></tr><tr><td>Emonts, Marieke</td><td>P-149</td></tr><tr><td>Engebraaten, Olav</td><td>P-47</td></tr><tr><td>Engel, Erwan</td><td>P-123</td></tr><tr><td>Engel, Jasper</td><td>P-292</td></tr><tr><td>Engelen, Marc</td><td>P-167</td></tr><tr><td>Engelke, Udo</td><td>11A</td></tr><tr><td>Engelke, Udo</td><td>P-576</td></tr><tr><td>Engelke, Udo, FH</td><td>P-80</td></tr><tr><td>Engelke, Udo, FH</td><td>P-265</td></tr><tr><td>Engelmann, Beatrice</td><td>P-317*</td></tr><tr><td>Engelsen, Søren, Balling</td><td>15E</td></tr><tr><td>Engelsen, Søren, Balling</td><td>P-1</td></tr><tr><td>Engelsen, Søren, Balling</td><td>P-395</td></tr><tr><td>Erbes, Thalia</td><td>P-50</td></tr><tr><td>Erdem, Yasemin</td><td>23E</td></tr><tr><td>Erdem, Yasemin</td><td>P-185</td></tr></tbody></table>

<table><thead><tr><th>Name Last, First</th><th>Abstracts</th></tr></thead><tbody><tr><td colspan="2">* = Presenting Author</td></tr><tr><td>Erjavec, Gordana, Nedic</td><td>P-92</td></tr><tr><td>Ernst, Madeleine</td><td>15C*</td></tr><tr><td>Ernst, Madeleine</td><td>P-544</td></tr><tr><td>Eschenlauer, Arthur</td><td>P-499*</td></tr><tr><td>Esko, Tonu</td><td>5D</td></tr><tr><td>Esler, Mark</td><td>P-499</td></tr><tr><td>Estoque, Jesse</td><td>1E</td></tr><tr><td>Etemadi, Arash</td><td>P-291</td></tr><tr><td>Euceda, Leslie, R.</td><td>P-47</td></tr><tr><td>Euceda, Leslie, R.</td><td>P-221</td></tr><tr><td>Eussen, Simone</td><td>5B</td></tr><tr><td>Evans, Annie</td><td>P-600</td></tr><tr><td>Evans, Charles</td><td>P-147</td></tr><tr><td>Evans, Ron</td><td>28A</td></tr><tr><td>Evelingen, Ellen</td><td>18B</td></tr><tr><td>Evelo, Chris</td><td>3C</td></tr><tr><td>Evelo, Chris, T.</td><td>P-471</td></tr><tr><td>Evich, Marina</td><td>P-315</td></tr><tr><td>Eyal, Stav</td><td>P-384</td></tr><tr><td>Eyres, Graham</td><td>18A</td></tr><tr><td>Ezernieks, Vilnis</td><td>22A</td></tr><tr><td>F, Borgan</td><td>P-95</td></tr><tr><td>F, Stassen</td><td>P-131</td></tr><tr><td>Faccio, Andréa</td><td>22B</td></tr><tr><td>Faccio, Andréa</td><td>P-75</td></tr><tr><td>Falk, Kevin, C.</td><td>P-369</td></tr><tr><td>Fan, Sili</td><td>P-493</td></tr><tr><td>Farah, Joao, P.S.</td><td>22B</td></tr><tr><td>Fardus-Reid, Fahmina</td><td>P-139</td></tr><tr><td>Farré, Marinella</td><td>P-302</td></tr><tr><td>Fassnacht, Martin</td><td>P-80</td></tr><tr><td>Fathima, Artnice</td><td>P-373*</td></tr><tr><td>Fauconneau, Benoit</td><td>P-396</td></tr><tr><td>Fearnley, Hugo</td><td>P-134</td></tr><tr><td>Fearnley, James</td><td>P-134</td></tr><tr><td>Feist, Adam, M.</td><td>P-386</td></tr><tr><td>Feistel, Felix</td><td>P-446</td></tr><tr><td>Fellinger, Paul</td><td>P-231</td></tr><tr><td>Fenaille, François</td><td>P-136</td></tr><tr><td>Fenaille, François</td><td>P-155</td></tr><tr><td>Fenaille, François</td><td>P-464</td></tr><tr><td>Fensom, Georgina, K.</td><td>P-109</td></tr><tr><td>Ferdinandusse, Sacha</td><td>P-167</td></tr><tr><td>Ferdinandusse, Sacha</td><td>P-180</td></tr><tr><td>Fernandes, Ana, Paula, de, Godoy</td><td>P-75</td></tr><tr><td>Fernandes, Eduarda</td><td>P-272</td></tr></tbody></table>
<table><thead><tr><th>Name Last, First</th><th>Abstracts</th></tr></thead><tbody><tr><td colspan="2">* = Presenting Author</td></tr><tr><td>Fernandes, Jolyn</td><td>P-2</td></tr><tr><td>Fernandez, Celine</td><td>P-120</td></tr><tr><td>Fernández, Facundo, M.</td><td>P-26</td></tr><tr><td>Fernández, Facundo, M.</td><td>P-260</td></tr><tr><td>Fernández, Mónica</td><td>P-42</td></tr><tr><td>Fernández-García, Miguel</td><td>P-85</td></tr><tr><td>Fernando, Vargas</td><td>P-205</td></tr><tr><td>Ferrari, Pietro</td><td>6A</td></tr><tr><td>Ferrari, Pietro</td><td>P-109</td></tr><tr><td>Ferrari, Pietro</td><td>P-123</td></tr><tr><td>Ferrario, Manuela</td><td>P-3*</td></tr><tr><td>Ferrer, Daniel, Lopez</td><td>P-277</td></tr><tr><td>Ferro, Valerie, A.</td><td>P-134</td></tr><tr><td>Feskens, Edith, J.M.</td><td>P-118</td></tr><tr><td>Feussner, Ivo</td><td>P-258</td></tr><tr><td>Fia, Massimo</td><td>P-350</td></tr><tr><td>Fiamoncini, Jarlei</td><td>P-181*</td></tr><tr><td>Fiamoncini, Jarlei</td><td>P-168</td></tr><tr><td>Fiedler, Martin, G.</td><td>P-560</td></tr><tr><td>Fiedler, Martin, G.</td><td>P-568</td></tr><tr><td>Fiehn, Oliver</td><td>11B</td></tr><tr><td>Fiehn, Oliver</td><td>P-79</td></tr><tr><td>Fiehn, Oliver</td><td>P-257</td></tr><tr><td>Fiehn, Oliver</td><td>P-443</td></tr><tr><td>Fiehn, Oliver</td><td>P-493</td></tr><tr><td>Filgueiras, Camila</td><td>14E*</td></tr><tr><td>Filgueiras, Camila, C.</td><td>P-437</td></tr><tr><td>Finch, Jasen</td><td>14D*</td></tr><tr><td>Fine, Dennis</td><td>2B</td></tr><tr><td>Finney, Kieran</td><td>P-107</td></tr><tr><td>Fischer, Krista</td><td>5D</td></tr><tr><td>Fischer, Steve</td><td>1B</td></tr><tr><td>Fischer, Steve</td><td>P-600</td></tr><tr><td>Fischer, Steven</td><td>P-586*</td></tr><tr><td>Fischer, Steven</td><td>P-293</td></tr><tr><td>Fischer, Steven</td><td>P-89</td></tr><tr><td>Fltzgerald, Melissa</td><td>P-365*</td></tr><tr><td>Fitzgerald, Melissa</td><td>P-359</td></tr><tr><td>Fitzgerald, Melissa</td><td>P-453</td></tr><tr><td>Fitzsimmons, Carolyn</td><td>P-469</td></tr><tr><td>Fjeldsted, John</td><td>30C</td></tr><tr><td>Flandin, Amelie</td><td>P-422</td></tr><tr><td>Flandin, Amélie</td><td>P-444</td></tr><tr><td>Fleischauer, Markus</td><td>P-539</td></tr><tr><td>Fleming, Ronan</td><td>17C*</td></tr></tbody></table>

<table><thead><tr><th>Name Last, First</th><th>Abstracts</th></tr></thead><tbody><tr><td>Fleming, Ronan, M.T.</td><td>17C</td></tr><tr><td>Fleming, Ronan, M.T.</td><td>27B</td></tr><tr><td>Fleming, Ronan, M.T.</td><td>P-199</td></tr><tr><td>Fleming, Ronan, M.T.</td><td>P-289</td></tr><tr><td>Fleming, Ronan, M.T.</td><td>P-268</td></tr><tr><td>Fleming, Ronan, M.T.</td><td>P-482</td></tr><tr><td>Flight, Robert, M.</td><td>P-43</td></tr><tr><td>Flitman, Reyhan, Sonmez</td><td>P-255*</td></tr><tr><td>Flores, Roberto</td><td>P-600</td></tr><tr><td>Flynn, Thomas</td><td>P-600</td></tr><tr><td>Focant, Jean-Francois</td><td>4C</td></tr><tr><td>Fochi, Igor</td><td>P-527</td></tr><tr><td>Fogelholm, Leila</td><td>P-352</td></tr><tr><td>Fomsgaard, Inge</td><td>P-449</td></tr><tr><td>Fomsgaard, Inge</td><td>P-443</td></tr><tr><td>Fomsgaard, Inge</td><td>P-450</td></tr><tr><td>Fongen, Monica</td><td>P-434</td></tr><tr><td>Fonteh, Alfred, N.</td><td>P-554</td></tr><tr><td>Fornace,, Jr., Albert</td><td>P-220*</td></tr><tr><td>Fornace,, Jr., Albert</td><td>P-220</td></tr><tr><td>Forné, Ignasi</td><td>28B</td></tr><tr><td>Foroutan, Aidin</td><td>P-469*</td></tr><tr><td>Fortney, Kristen</td><td>5D</td></tr><tr><td>Fortuny, Abel, Folch</td><td>P-371</td></tr><tr><td>Foster, Neil</td><td>P-107</td></tr><tr><td>Four, Emine, Kazanc</td><td>P-59</td></tr><tr><td>Fox, Bennett</td><td>P-260</td></tr><tr><td>Fragner, Lena</td><td>P-448*</td></tr><tr><td>Franco, Antonio</td><td>P-350</td></tr><tr><td>Franco, Daniel</td><td>P-42</td></tr><tr><td>Frank, Daniel, N.</td><td>P-122</td></tr><tr><td>Franschitz, EvaMaria</td><td>P-91</td></tr><tr><td>Fraser, Andy, G.</td><td>P-393</td></tr><tr><td>Fraser-Caris, Robert</td><td>P-585</td></tr><tr><td>Frechen, M.A.</td><td>P-409</td></tr><tr><td>Frederic, De-Bock</td><td>P-325</td></tr><tr><td>Frederic, Lasserre</td><td>P-325</td></tr><tr><td>Freedman, Neal</td><td>6A</td></tr><tr><td>Freiburghaus, Katrin</td><td>P-568*</td></tr><tr><td>Freiburghaus, Katrin</td><td>P-560</td></tr><tr><td>Freisling, Heinz</td><td>P-341</td></tr><tr><td>Frentzel, Stefan</td><td>P-312</td></tr><tr><td>Frey, Maximilian</td><td>11B</td></tr><tr><td>Friedecký, David</td><td>P-164*</td></tr><tr><td>Friedecký, David</td><td>P-163</td></tr><tr><td>Friedecký, David</td><td>P-320</td></tr><tr><td>Fritsche, Raphaela</td><td>P-86*</td></tr></tbody></table>

<table><thead><tr><th>Name Last, First</th><th>Abstracts</th></tr></thead><tbody><tr><td>Fritsche, Raphaela</td><td>P-483</td></tr><tr><td>Fritsche, Raphaela</td><td>P-552</td></tr><tr><td>Fritz-Wallace, Katarina</td><td>P-317</td></tr><tr><td>Frolov, Andrej</td><td>11B</td></tr><tr><td>Frost, Gary</td><td>P-329</td></tr><tr><td>Frost, Gary</td><td>P-181</td></tr><tr><td>Frost, Gary</td><td>P-339</td></tr><tr><td>Frost, Gary</td><td>P-343</td></tr><tr><td>Fu, Jingyuan</td><td>28C</td></tr><tr><td>Fu, Ting</td><td>28A</td></tr><tr><td>Fuchs, Charles, S.</td><td>P-22</td></tr><tr><td>Fuchs, Regine</td><td>P-327*</td></tr><tr><td>Fujimoto, Hirotaka</td><td>P-178</td></tr><tr><td>Fujita, Yoshihito</td><td>P-356*</td></tr><tr><td>Fujita, Yoshihito</td><td>P-366</td></tr><tr><td>Fujiwara, Toshimichi</td><td>P-494</td></tr><tr><td>Fukusaki, Eiichiro</td><td>P-361*</td></tr><tr><td>Fukusaki, Eiichiro</td><td>P-200</td></tr><tr><td>Fukusaki, Eiichiro</td><td>P-364</td></tr><tr><td>Fukusaki, Eiichiro</td><td>P-373</td></tr><tr><td>Fukusaki, Eiichiro</td><td>P-379</td></tr><tr><td>Fukusaki, Eiichiro</td><td>P-428</td></tr><tr><td>Fukushima, Atsushi</td><td>19B*</td></tr><tr><td>Fukushima, Atsushi</td><td>P-556</td></tr><tr><td>Fürst, Susanne</td><td>P-613*</td></tr><tr><td>Furtado, Danielle, Zildeana, Sousa</td><td>P-78*</td></tr><tr><td>Furuno, Masahiro</td><td>P-379</td></tr><tr><td>Furuya, Futoshi</td><td>P-356</td></tr><tr><td>Furuya, Futoshi</td><td>P-366</td></tr><tr><td>G, Balcke</td><td>P-286</td></tr><tr><td>G, Sassano</td><td>P-179</td></tr><tr><td>Gabilondo, Alfredo, Ibañez</td><td>P-330</td></tr><tr><td>Gahagen, Janet</td><td>P-220</td></tr><tr><td>Gaillard, Romy</td><td>P-249</td></tr><tr><td>Galbiatti, Maria, Isabel</td><td>P-417</td></tr><tr><td>Galindo-Prieto, Beatriz</td><td>P-162*</td></tr><tr><td>Galineau, Laurent</td><td>P-87</td></tr><tr><td>Galineau, Laurent</td><td>P-234</td></tr><tr><td>Gallagher, James</td><td>5C</td></tr><tr><td>Gallart-Ayala, Héctor</td><td>P-14</td></tr><tr><td>Gallego, M., Joan, S.</td><td>P-279*</td></tr><tr><td>Gama-Perez, Pau</td><td>28B</td></tr><tr><td>Gamet-Payrastre, Laurence</td><td>P-160</td></tr><tr><td>Gangu, Vamshidhar</td><td>P-305</td></tr><tr><td>Gao, He</td><td>9B</td></tr></tbody></table>

<table><thead><tr><th>Name Last, First</th><th>Abstracts</th></tr></thead><tbody><tr><td>Gaquerel, Emmanuel</td><td>11B</td></tr><tr><td>Garbeva, Paolina</td><td>P-383</td></tr><tr><td>García, Arnaud</td><td>P-535</td></tr><tr><td>Garcia, Brianna</td><td>26A</td></tr><tr><td>Garcia, Brianna</td><td>P-260*</td></tr><tr><td>Garcia-Aloy, Mar</td><td>P-350*</td></tr><tr><td>Garcia-Aloy, Mar</td><td>P-351</td></tr><tr><td>Garcia-Altares, Maria</td><td>P-519</td></tr><tr><td>Garcia-Mantrana, Izaskun</td><td>P-347</td></tr><tr><td>Garcia-Perez, Isabel</td><td>P-343</td></tr><tr><td>Garcia-Roves, Pablo</td><td>28B</td></tr><tr><td>García-Verdejo, Francisco, José</td><td>P-42</td></tr><tr><td>Garg, Neeraj</td><td>4B</td></tr><tr><td>Garred, Øystein</td><td>P-47</td></tr><tr><td>Garrett, Timothy</td><td>P-225</td></tr><tr><td>Garrett, Timothy</td><td>P-211</td></tr><tr><td>Garrett, Timothy</td><td>P-288</td></tr><tr><td>Garrett, Timothy</td><td>P-555</td></tr><tr><td>Gatto, Laurent</td><td>P-496</td></tr><tr><td>Gaudreau, Pierrette</td><td>P-13</td></tr><tr><td>Gaudreau, Pierrette</td><td>P-155</td></tr><tr><td>Gaudreau, Pierrette</td><td>P-501</td></tr><tr><td>Gaul, David</td><td>P-26*</td></tr><tr><td>Gauvin, John</td><td>P-371*</td></tr><tr><td>Gauvin, John</td><td>P-516</td></tr><tr><td>Gay, Melvin</td><td>21E</td></tr><tr><td>Gdowicz-Kłosok, Agnieszka</td><td>P-19</td></tr><tr><td>Geddes, Donna</td><td>21E</td></tr><tr><td>Geerinckx, Suzy</td><td>P-614</td></tr><tr><td>Gehant, Sebastien</td><td>19A</td></tr><tr><td>Geijsen, Anne, J.M.R.</td><td>P-66</td></tr><tr><td>Gelhaus, Barbara</td><td>P-181</td></tr><tr><td>Geng, Dawei</td><td>P-301</td></tr><tr><td>Genilloud, Olga</td><td>P-42</td></tr><tr><td>George, Alexandra</td><td>21E*</td></tr><tr><td>Gering, Albert</td><td>P-210</td></tr><tr><td>Geris, L.</td><td>12E</td></tr><tr><td>Gerl, Mathias, J</td><td>P-120</td></tr><tr><td>Gerlich, Silke, C.</td><td>P-420</td></tr><tr><td>Germain, Arnaud</td><td>P-215*</td></tr><tr><td>Germond, Arno</td><td>16C</td></tr><tr><td>Gershenzon, Jonathan</td><td>P-446</td></tr><tr><td>Gethings, Lee</td><td>P-63</td></tr><tr><td>Gethings, Lee</td><td>P-254</td></tr><tr><td>Ghanbari, Reza</td><td>P-291</td></tr></tbody></table>
<table><thead><tr><th>Name Last, First</th><th>Abstracts</th></tr></thead><tbody><tr><td colspan="2">* = Presenting Author</td></tr><tr><td>Gharios, George</td><td>5C</td></tr><tr><td>Ghesquiere, B.</td><td>12E</td></tr><tr><td>Ghesquière, Bart</td><td>P-614</td></tr><tr><td>Ghosh, Nilanjana</td><td>25B*</td></tr><tr><td>Ghosh, Nilanjana</td><td>P-253</td></tr><tr><td>Ghesson, Hikmat</td><td>P-303</td></tr><tr><td>Giacomoni, Franck</td><td>P-549</td></tr><tr><td>Giallourou, Natasa</td><td>P-139*</td></tr><tr><td>Gibbons, Helena</td><td>18C</td></tr><tr><td>Gibbons, Helena</td><td>P-339</td></tr><tr><td>Gibon, Yves</td><td>P-422</td></tr><tr><td>Gibon, Yves</td><td>P-444</td></tr><tr><td>Gibson, Glenn</td><td>P-388</td></tr><tr><td>Gicquiau, Audrey</td><td>P-109</td></tr><tr><td>Gieger, Christian</td><td>P-158</td></tr><tr><td>Gieger, Christian</td><td>P-188</td></tr><tr><td>Giesbertz, Pieter</td><td>P-168*</td></tr><tr><td>Gigic, Biljana</td><td>P-66</td></tr><tr><td>Gika, Helen</td><td>P-110</td></tr><tr><td>Giles, Corey</td><td>13B</td></tr><tr><td>Giles, Tim</td><td>P-107</td></tr><tr><td>Gill, Kirandeep</td><td>P-220</td></tr><tr><td>Gilmore, Ian</td><td>P-59</td></tr><tr><td>Giménez, María, Isabel</td><td>P-48</td></tr><tr><td>Giordano, Giuseppe</td><td>P-247*</td></tr><tr><td>Giovannucci, Edward</td><td>P-22</td></tr><tr><td>Giovati, Laura</td><td>P-387</td></tr><tr><td>Giskeødegård, Guro</td><td>P-20</td></tr><tr><td>Giskeødegård, Guro</td><td>P-221*</td></tr><tr><td>Giskeødegård, Guro</td><td>P-47</td></tr><tr><td>Giskeødegård, Guro</td><td>P-67</td></tr><tr><td>Giuberti, Gianluca</td><td>P-337</td></tr><tr><td>Glaab, Enrico</td><td>17C</td></tr><tr><td>Glaab, Enrico</td><td>27B</td></tr><tr><td>Glatter, Timo</td><td>3D</td></tr><tr><td>Gleizes, Anne</td><td>19A</td></tr><tr><td>Glen, Robert, C</td><td>P-171</td></tr><tr><td>Gloaguen, Yoann</td><td>P-549*</td></tr><tr><td>Gloaguen, Yoann</td><td>P-86</td></tr><tr><td>Gloaguen, Yoann</td><td>P-483</td></tr><tr><td>Globisch, Daniel</td><td>4B*</td></tr><tr><td>Glushka, John</td><td>P-264</td></tr><tr><td>Gmelch, Lena</td><td>P-389*</td></tr><tr><td>Go, Young-Mi</td><td>24A</td></tr><tr><td>Go, Young-Mi</td><td>P-2*</td></tr><tr><td>Godejohann, Markus</td><td>P-196</td></tr><tr><td>Godzien, Joanna</td><td>21C*</td></tr><tr><td>Godzien, Joanna</td><td>P-40</td></tr></tbody></table>

<table><thead><tr><th>Name Last, First</th><th>Abstracts</th></tr></thead><tbody><tr><td colspan="2">* = Presenting Author</td></tr><tr><td>Goeminne, Geert</td><td>11B</td></tr><tr><td>Goeminne, Geert</td><td>P-424</td></tr><tr><td>Goeminne, Geert</td><td>P-431</td></tr><tr><td>Goeminne, Geert</td><td>P-556</td></tr><tr><td>Goethals, Sophie</td><td>P-126*</td></tr><tr><td>Goetz, Sebastian</td><td>P-508</td></tr><tr><td>Gogiashvili, Mikheil</td><td>P-57*</td></tr><tr><td>Gogiashvili, Mikheil</td><td>P-269</td></tr><tr><td>Gogonea, Valentin</td><td>P-79</td></tr><tr><td>Goh, Jorming</td><td>P-143</td></tr><tr><td>Goicolea, M., Aránzazu</td><td>P-242</td></tr><tr><td>Goldansaz, Seyed, Ali</td><td>P-400*</td></tr><tr><td>Golla, Jaya, Prakash</td><td>P-108</td></tr><tr><td>Gómez, Gerard, Baquer</td><td>P-519*</td></tr><tr><td>Gomez, Manuel</td><td>P-293</td></tr><tr><td>Gómez-Cadenas, Aurelio</td><td>P-408</td></tr><tr><td>Gong, Ryong</td><td>P-411</td></tr><tr><td>Goñi, Fernando</td><td>P-227</td></tr><tr><td>Gonsalves, Wilson</td><td>P-46*</td></tr><tr><td>Gontier, Eric</td><td>29B</td></tr><tr><td>Gonzales, Gerard</td><td>3E*</td></tr><tr><td>Gonzalez, Clicerio</td><td>5D</td></tr><tr><td>Gonzalez, Maria</td><td>5D</td></tr><tr><td>González, Oskar</td><td>P-243*</td></tr><tr><td>González, Oskar</td><td>P-244</td></tr><tr><td>González-Domínguez, Raul</td><td>P-351</td></tr><tr><td>González-Domínguez, Raúl</td><td>6C*</td></tr><tr><td>González-Domínguez, Raúl</td><td>9C</td></tr><tr><td>Gonzalez-Pena, Diana</td><td>18C</td></tr><tr><td>Gonzalez-Riaño, Carolina</td><td>P-82</td></tr><tr><td>González-Ruiz, Víctor</td><td>P-535</td></tr><tr><td>Goo, Hyeyoon</td><td>P-144</td></tr><tr><td>Goodacre, Roy</td><td>17B</td></tr><tr><td>Goodacre, Roy</td><td>P-6</td></tr><tr><td>Goodacre, Royston</td><td>P-243</td></tr><tr><td>Goodacre, Royston</td><td>P-244</td></tr><tr><td>Goodwin, Richard</td><td>P-59</td></tr><tr><td>Gooley, Andrew</td><td>P-579</td></tr><tr><td>Goossens, Alain</td><td>11B</td></tr><tr><td>Goossens, Gijs</td><td>5B</td></tr><tr><td>Goracci, Laura</td><td>P-547</td></tr><tr><td>Gorji, Sara, Ghorbani</td><td>P-359*</td></tr><tr><td>Gorji, Sara, Ghorbani</td><td>P-365</td></tr></tbody></table>

<table><thead><tr><th>Name Last, First</th><th>Abstracts</th></tr></thead><tbody><tr><td colspan="2">* = Presenting Author</td></tr><tr><td>Gormaz, María</td><td>P-203</td></tr><tr><td>Gormaz, María</td><td>P-347</td></tr><tr><td>Gorti, Santosh, Kapil, Kumar</td><td>P-587*</td></tr><tr><td>Gorti, Santosh, Kapil, Kumar</td><td>P-605</td></tr><tr><td>Goto, Tsuyoshi</td><td>P-356</td></tr><tr><td>Goto, Tsuyoshi</td><td>P-366</td></tr><tr><td>Götz, Lou</td><td>19A</td></tr><tr><td>Gouis, Jacques, Le</td><td>P-422</td></tr><tr><td>Goujon, Catherine</td><td>P-312</td></tr><tr><td>Gouveia, Goncalo</td><td>P-390*</td></tr><tr><td>Gouveia, Gonçalo</td><td>26A</td></tr><tr><td>Gouveia, Goncalo, J.</td><td>P-260</td></tr><tr><td>Govaerts, B.</td><td>P-4</td></tr><tr><td>Govaerts, Bernadette</td><td>P-15</td></tr><tr><td>Govind, Niranjan</td><td>P-297</td></tr><tr><td>Govind, Niranjan</td><td>P-557</td></tr><tr><td>Gowda, G.A., Nagana</td><td>P-531</td></tr><tr><td>Graca, Goncalo</td><td>19E</td></tr><tr><td>Graça, Gonçalo</td><td>9B</td></tr><tr><td>Graça, Gonçalo</td><td>P-559*</td></tr><tr><td>Graça, Gonçalo</td><td>P-248</td></tr><tr><td>Graça, Gonçalo</td><td>P-475</td></tr><tr><td>Grad, Yonatan</td><td>P-141</td></tr><tr><td>Granell, Antonio</td><td>2A*</td></tr><tr><td>Granell, Antonio</td><td>P-408</td></tr><tr><td>Grauslys, Arturas</td><td>P-486</td></tr><tr><td>Gray, Nicola</td><td>P-388*</td></tr><tr><td>Gregor, Rachel</td><td>P-384*</td></tr><tr><td>Grieser, Charles</td><td>P-600</td></tr><tr><td>Griffin, Julian</td><td>30C</td></tr><tr><td>Griffin, Julian</td><td>P-171*</td></tr><tr><td>Griffin, Timothy</td><td>P-499</td></tr><tr><td>Grigoryan, Hasmik</td><td>P-28</td></tr><tr><td>Grinde, Maria, T.</td><td>P-270*</td></tr><tr><td>Groff, Nelli</td><td>P-350</td></tr><tr><td>Grönbladh, Alfhild</td><td>P-187</td></tr><tr><td>Gronwald, Wolfram</td><td>P-228</td></tr><tr><td>Groop, Leif</td><td>P-176</td></tr><tr><td>Grosjean, Jérémy</td><td>11B</td></tr><tr><td>Gross, Steven</td><td>1B*</td></tr><tr><td>Gross, Steven, S.</td><td>P-64</td></tr><tr><td>Gruber, Margaret, Y.</td><td>P-369</td></tr><tr><td>Gsur, Andrea</td><td>P-66</td></tr><tr><td>Guan, Leluo</td><td>P-469</td></tr><tr><td>Guardi, Manuela</td><td>P-226</td></tr><tr><td>Guasch, Marta</td><td>P-480</td></tr></tbody></table>

<table><thead><tr><th>Name Last, First</th><th>Abstracts</th></tr></thead><tbody><tr><td colspan="2">* = Presenting Author</td></tr><tr><td>Gucciardi, Antonella</td><td>P-247</td></tr><tr><td>Guercio, Brendan, J.</td><td>P-22</td></tr><tr><td>Guest, Miriam</td><td>10C</td></tr><tr><td>Guillon, Blanche</td><td>P-464</td></tr><tr><td>Guillou, Hervé</td><td>P-160</td></tr><tr><td>Guinta, Allison</td><td>P-201</td></tr><tr><td>Guitton, Yann</td><td>P-571*</td></tr><tr><td>Guitton, Yann</td><td>P-549</td></tr><tr><td>Gulde, Paul</td><td>P-277</td></tr><tr><td>Guled, Faisa</td><td>P-569</td></tr><tr><td>Gulersonmez, Can</td><td>17C</td></tr><tr><td>Gulston, Melanie, K.</td><td>P-171</td></tr><tr><td>Gumpenberger, Tanja</td><td>P-66</td></tr><tr><td>Gunter, Marc</td><td>P-341</td></tr><tr><td>Gunter, Marc, J.</td><td>P-109</td></tr><tr><td>Gunter, Marc, J.</td><td>P-123</td></tr><tr><td>Guoyun, Xu</td><td>P-416</td></tr><tr><td>Gürdeniz, Gözde</td><td>P-332</td></tr><tr><td>Gürtl, Bettina</td><td>21D</td></tr><tr><td>Gürtl, Bettina</td><td>P-231*</td></tr><tr><td>Gurusinghe, Saliya</td><td>P-458</td></tr><tr><td>Guy, P.A.</td><td>P-529</td></tr><tr><td>Guy, P.A.</td><td>P-565</td></tr><tr><td>Guzylack, Laurence</td><td>P-160</td></tr><tr><td>Gwon, Mi-Ri</td><td>P-101*</td></tr><tr><td>H, Laurikainen</td><td>P-95</td></tr><tr><td>H, Treutler</td><td>P-286</td></tr><tr><td>Haag, Alexander</td><td>P-168</td></tr><tr><td>Haag, Alexander</td><td>P-181</td></tr><tr><td>Haag, Mathias</td><td>P-276</td></tr><tr><td>Haake, Volker</td><td>P-327</td></tr><tr><td>Habermann, Nina</td><td>P-66</td></tr><tr><td>Hachi, Siham</td><td>17C</td></tr><tr><td>Hachi, Siham</td><td>P-199</td></tr><tr><td>Hackbusch, Sven</td><td>P-588</td></tr><tr><td>Hackbusch, Sven</td><td>P-589</td></tr><tr><td>Hageman, Jos</td><td>2D</td></tr><tr><td>Hageman, Jos</td><td>P-451</td></tr><tr><td>Haid, Mark</td><td>P-111*</td></tr><tr><td>Haiduc, A.</td><td>P-565</td></tr><tr><td>Haiduc, Adrian</td><td>P-529*</td></tr><tr><td>Haiman, Christopher</td><td>P-39</td></tr><tr><td>Hakonen, Anna</td><td>24B</td></tr><tr><td>Halabalaki, Maria</td><td>11B</td></tr><tr><td>Halabalaki, Maria</td><td>P-394*</td></tr><tr><td>Halabalaki, Maria</td><td>P-281</td></tr><tr><td>Halama, Anna</td><td>P-64*</td></tr><tr><td>Halde, Rashi</td><td>27B</td></tr></tbody></table>
<table><thead><tr><th>Name Last, First</th><th>Abstracts</th></tr></thead><tbody><tr><td colspan="2">* = Presenting Author</td></tr><tr><td>Halford, S.</td><td>P-25</td></tr><tr><td>Hall, G., Nisson</td><td>12E</td></tr><tr><td>Hall, Robert</td><td>14A</td></tr><tr><td>Hall, Robert</td><td>2D*</td></tr><tr><td>Hall, Robert</td><td>P-399</td></tr><tr><td>Hall, Robert</td><td>P-457</td></tr><tr><td>Hall, Robert</td><td>P-357</td></tr><tr><td>Hallberg, Mathias</td><td>P-187</td></tr><tr><td>Halldórsson, R., S., Sigurðardóttir, S.</td><td>P-222</td></tr><tr><td>Halle, Martin</td><td>P-111</td></tr><tr><td>Haller, Dirk</td><td>18E</td></tr><tr><td>Hallet, Jason</td><td>P-398</td></tr><tr><td>Hallmans, Göran</td><td>9D</td></tr><tr><td>Hallmans, Göran</td><td>P-124</td></tr><tr><td>Hällqvist, Jenny</td><td>P-475</td></tr><tr><td>Ham, Hyeonmi</td><td>P-299*</td></tr><tr><td>Ham, Hyeonmi</td><td>P-342</td></tr><tr><td>Hamano, Fumie</td><td>P-212</td></tr><tr><td>Han, Dae, Hee</td><td>P-204</td></tr><tr><td>Han, Jun</td><td>P-537</td></tr><tr><td>Han, Seung, Seok</td><td>P-224</td></tr><tr><td>Han, Ting</td><td>P-239*</td></tr><tr><td>Hanazaki, Kazuhiro</td><td>P-229</td></tr><tr><td>Hancock, Sarah</td><td>1E*</td></tr><tr><td>Hanhineva, Kati</td><td>18D*</td></tr><tr><td>Hanhineva, Kati</td><td>P-115</td></tr><tr><td>Hanhineva, Kati</td><td>P-116</td></tr><tr><td>Hanhineva, Kati</td><td>P-129</td></tr><tr><td>Hanhineva, Kati</td><td>P-159</td></tr><tr><td>Hanhineva, Kati</td><td>P-382</td></tr><tr><td>Hanhineva, Kati</td><td>P-536</td></tr><tr><td>Hankemeier, Thomas</td><td>12C</td></tr><tr><td>Hankemeier, Thomas</td><td>16C</td></tr><tr><td>Hankemeier, Thomas</td><td>17C</td></tr><tr><td>Hankemeier, Thomas</td><td>18B</td></tr><tr><td>Hankemeier, Thomas</td><td>23D</td></tr><tr><td>Hankemeier, Thomas</td><td>27B</td></tr><tr><td>Hankemeier, Thomas</td><td>4A*</td></tr><tr><td>Hankemeier, Thomas</td><td>5B</td></tr><tr><td>Hankemeier, Thomas</td><td>P-33</td></tr><tr><td>Hankemeier, Thomas</td><td>P-97</td></tr><tr><td>Hankemeier, Thomas</td><td>P-151</td></tr><tr><td>Hankemeier, Thomas</td><td>P-199</td></tr><tr><td>Hankemeier, Thomas</td><td>P-268</td></tr><tr><td>Hankemeier, Thomas</td><td>P-289</td></tr><tr><td>Hankemeier, Thomas</td><td>P-566</td></tr><tr><td>Hankemeier, Thomas</td><td>P-569</td></tr></tbody></table>

<table><thead><tr><th>Name Last, First</th><th>Abstracts</th></tr></thead><tbody><tr><td colspan="2">* = Presenting Author</td></tr><tr><td>Hankemeier, Thomas</td><td>P-570</td></tr><tr><td>Hankemeier, Thomas</td><td>P-584</td></tr><tr><td>Hankemeier, Thomas</td><td>P-84</td></tr><tr><td>Hansen, Ailin, F.</td><td>P-20</td></tr><tr><td>Hansen, S.</td><td>P-4</td></tr><tr><td>Hansen, Torben</td><td>P-174</td></tr><tr><td>Hanson, Maureen, R.</td><td>P-215</td></tr><tr><td>Harada, Sei</td><td>P-100*</td></tr><tr><td>Harada, Sei</td><td>P-183</td></tr><tr><td>Harada, Sei</td><td>P-509</td></tr><tr><td>Haraldsdóttir, Hulda</td><td>17C</td></tr><tr><td>Haraldsson, G.G.</td><td>P-222</td></tr><tr><td>Hardy, Nigel, W</td><td>P-259</td></tr><tr><td>Harflett, Claudia</td><td>29C</td></tr><tr><td>Harms, Amy</td><td>17C</td></tr><tr><td>Harms, Amy</td><td>18B</td></tr><tr><td>Harms, Amy</td><td>27B</td></tr><tr><td>Harms, Amy</td><td>P-151</td></tr><tr><td>Harms, Amy</td><td>P-289</td></tr><tr><td>Harms, Amy</td><td>P-33</td></tr><tr><td>Harms, Amy</td><td>P-84</td></tr><tr><td>Harms, Amy</td><td>P-268</td></tr><tr><td>Harms, Amy</td><td>P-566</td></tr><tr><td>Harnett, William</td><td>P-134</td></tr><tr><td>Harnphadungkit, Kamontip</td><td>P-456</td></tr><tr><td>Harrington, Michael, G.</td><td>P-554</td></tr><tr><td>Harris, Linda</td><td>P-461</td></tr><tr><td>Harrison, Mark</td><td>P-398</td></tr><tr><td>Hartmann, Mikael</td><td>P-143</td></tr><tr><td>Hartstra, Annick</td><td>6B</td></tr><tr><td>Hartung, Thomas</td><td>P-600</td></tr><tr><td>Hartvigsson, Olle</td><td>P-235*</td></tr><tr><td>Harvey, David</td><td>P-406</td></tr><tr><td>Harwood-Stamper, Annie</td><td>P-603*</td></tr><tr><td>Harynuk, James</td><td>P-582*</td></tr><tr><td>Harynuk, James</td><td>P-537</td></tr><tr><td>Harynuk, James</td><td>P-558</td></tr><tr><td>Harynuk, James</td><td>P-284</td></tr><tr><td>Haryono, Mindia, A.S.</td><td>10D</td></tr><tr><td>Hashimoto, Kei</td><td>P-521</td></tr><tr><td>Haslauer, Kristina</td><td>P-597*</td></tr><tr><td>Hassanpour, Neda</td><td>P-548</td></tr><tr><td>Hassoun, Soha</td><td>P-548*</td></tr><tr><td>Hasunuma, Tomohisa</td><td>10B*</td></tr><tr><td>Hatch, Marguerite</td><td>P-211</td></tr></tbody></table>

<table><thead><tr><th>Name Last, First</th><th>Abstracts</th></tr></thead><tbody><tr><td colspan="2">* = Presenting Author</td></tr><tr><td>Hattori, Ayuna</td><td>P-264</td></tr><tr><td>Hattori, Takanari</td><td>P-591*</td></tr><tr><td>Hattori, Takanari</td><td>P-200</td></tr><tr><td>Hattwell, Jake, P.N.</td><td>26B</td></tr><tr><td>Haug, Kenneth</td><td>3B</td></tr><tr><td>Haug, Kenneth</td><td>P-292</td></tr><tr><td>Haug, Kenneth</td><td>P-596</td></tr><tr><td>Haukaas, Tonje</td><td>P-221</td></tr><tr><td>Hauner, Hans</td><td>P-112</td></tr><tr><td>Häußler, M.</td><td>P-409</td></tr><tr><td>Hauton, David</td><td>P-611</td></tr><tr><td>Havulinna, Aki, S</td><td>P-120</td></tr><tr><td>Hawkins, Nathan</td><td>P-564*</td></tr><tr><td>Hayakawa, Yoshihiro</td><td>P-200</td></tr><tr><td>Hayasaka, Ryosuke</td><td>P-61</td></tr><tr><td>Hayashi, Kuniyoshi</td><td>P-178</td></tr><tr><td>Hayati, Sri</td><td>P-361</td></tr><tr><td>Hazen, Stanley, L.</td><td>P-79</td></tr><tr><td>Haznadar, Majda</td><td>P-600</td></tr><tr><td>He, Yupeng</td><td>23D</td></tr><tr><td>He, Zhaoyue</td><td>P-560</td></tr><tr><td>Hechinger, Ryan</td><td>P-441</td></tr><tr><td>Heland, Mikael</td><td>P-71</td></tr><tr><td>Heland, Mikael</td><td>P-187</td></tr><tr><td>Hedemann, Mette, Sko</td><td>P-191</td></tr><tr><td>Hedenbro, Jan</td><td>P-176</td></tr><tr><td>Heeren, Ron</td><td>P-67</td></tr><tr><td>Hefner, Ying</td><td>P-386</td></tr><tr><td>Hegeman, Adrian</td><td>P-499</td></tr><tr><td>Heike, Yuji</td><td>P-178</td></tr><tr><td>Heiner-Fokkema, Rebecca</td><td>P-210</td></tr><tr><td>Heinonen, Jari</td><td>6E</td></tr><tr><td>Heinzmann, Silke</td><td>18E</td></tr><tr><td>Heinzmann, Silke</td><td>P-550*</td></tr><tr><td>Heinzmann, Silke</td><td>P-597</td></tr><tr><td>Heirendt, Laurent</td><td>17C</td></tr><tr><td>Heischmann, Svenja</td><td>P-206</td></tr><tr><td>Heischmann, Svenja</td><td>P-573</td></tr><tr><td>Hejblum, Boris</td><td>9C</td></tr><tr><td>Helf, Maximilian</td><td>11C*</td></tr><tr><td>Helmer, Catherine</td><td>9C</td></tr><tr><td>Helmus, Rick</td><td>P-511*</td></tr><tr><td>HELMY, Nada</td><td>P-193</td></tr><tr><td>Hemeryck, Lieselot, Y.</td><td>P-126</td></tr><tr><td>Hemmler, Daniel</td><td>18E</td></tr><tr><td>Hemmler, Daniel</td><td>P-597</td></tr></tbody></table>

<table><thead><tr><th>Name Last, First</th><th>Abstracts</th></tr></thead><tbody><tr><td colspan="2">* = Presenting Author</td></tr><tr><td>Hendricks, Audrey, E.</td><td>P-122</td></tr><tr><td>Hendriks, Margriet</td><td>P-371</td></tr><tr><td>Hendriks, Margriet</td><td>P-516</td></tr><tr><td>Hengerer, Bastian</td><td>17C</td></tr><tr><td>Henriksen, Hanne</td><td>13D</td></tr><tr><td>Henriksen, Hanne, Hee</td><td>P-285*</td></tr><tr><td>Henrique, Rui</td><td>P-23</td></tr><tr><td>Henriques, Luís, F.</td><td>P-543</td></tr><tr><td>Henry, Hugues</td><td>P-14</td></tr><tr><td>Henry, Ronald</td><td>5B</td></tr><tr><td>Herberg, Jethro</td><td>P-149</td></tr><tr><td>Herberth, Gunda</td><td>P-317</td></tr><tr><td>Herendi, Lili</td><td>1C*</td></tr><tr><td>Hergenröder, Roland</td><td>P-57</td></tr><tr><td>Hergenröder, Roland</td><td>P-269</td></tr><tr><td>Herman, Stephanie</td><td>11B</td></tr><tr><td>Herman, Stephanie</td><td>P-93*</td></tr><tr><td>Herman, Stephanie</td><td>P-197</td></tr><tr><td>Hermans, Anne</td><td>P-461</td></tr><tr><td>Hernandes, Vinicius, Veri</td><td>P-166*</td></tr><tr><td>Hernandes, Vinícius, Veri</td><td>P-417</td></tr><tr><td>Herpell, Johannes</td><td>P-448</td></tr><tr><td>Herr, Deron</td><td>22C</td></tr><tr><td>Herrington, David</td><td>19E</td></tr><tr><td>Herrington, David</td><td>9B</td></tr><tr><td>Herrington, David</td><td>P-475</td></tr><tr><td>Herrington, David</td><td>P-559</td></tr><tr><td>Herrington, David</td><td>P-600</td></tr><tr><td>Herve, Guillou</td><td>P-325</td></tr><tr><td>Herzog, Katharina</td><td>P-176*</td></tr><tr><td>Heux, Stéphanie</td><td>P-38</td></tr><tr><td>Heywood, David</td><td>P-583*</td></tr><tr><td>Hicks, Andrew</td><td>P-166</td></tr><tr><td>Hietala, Ari</td><td>P-434</td></tr><tr><td>Hietala, Jarmo</td><td>P-94</td></tr><tr><td>Higashi, Rick</td><td>P-600</td></tr><tr><td>Higashi, Yasuhiro</td><td>15B</td></tr><tr><td>Hill, Bradford, G.</td><td>P-505</td></tr><tr><td>Hill, Camilla, B.</td><td>P-258</td></tr><tr><td>Hiller, Karsten</td><td>P-338</td></tr><tr><td>Hillis, Graham</td><td>13B</td></tr><tr><td>Hinz, Christine</td><td>30C</td></tr><tr><td>Hinz, Christine</td><td>P-388</td></tr><tr><td>Hirakawa, Hideki</td><td>19B</td></tr><tr><td>Hirayama, Akiyoshi</td><td>P-61*</td></tr></tbody></table>
<table><thead><tr><th>Name Last, First</th><th>Abstracts</th></tr></thead><tbody><tr><td colspan="2">* = Presenting Author</td></tr><tr><td>Hirayama, Akiyoshi</td><td>P-100</td></tr><tr><td>Hirayama, Akiyoshi</td><td>P-340</td></tr><tr><td>Hirayama, Akiyoshi</td><td>P-509</td></tr><tr><td>Hirschberg, S.</td><td>P-25</td></tr><tr><td>Hirschhorn, Joel</td><td>5D</td></tr><tr><td>Hishinuma, Eiji</td><td>P-58*</td></tr><tr><td>Hishinuma, Eiji</td><td>P-105</td></tr><tr><td>Ho, Hui-Yu</td><td>P-59</td></tr><tr><td>Hoch, Jeffrey, C.</td><td>P-494</td></tr><tr><td>Hoeng, Julia</td><td>P-312</td></tr><tr><td>Hoevenaars, Femke</td><td>6B</td></tr><tr><td>Hofer, Rene</td><td>P-424</td></tr><tr><td>Hoffmann, Christian</td><td>P-181</td></tr><tr><td>Hoffmann, Martin, A.</td><td>P-539</td></tr><tr><td>Hofmann, Ute</td><td>P-276</td></tr><tr><td>Høiem, Therese, S.</td><td>P-20*</td></tr><tr><td>Høiem, Therese, S.</td><td>P-67</td></tr><tr><td>Holcapek, Michal</td><td>13A*</td></tr><tr><td>Holmes, Elaine</td><td>13C</td></tr><tr><td>Holmes, Elaine</td><td>P-329</td></tr><tr><td>Holmes, Elaine</td><td>P-248</td></tr><tr><td>Holmes, Elaine</td><td>P-343</td></tr><tr><td>Holowatyj, Andreana, N.</td><td>P-66</td></tr><tr><td>Holubčíková, Simona</td><td>P-65</td></tr><tr><td>Hon, Jason</td><td>P-579</td></tr><tr><td>Hong, Young-Shick</td><td>P-16</td></tr><tr><td>Hongisto, Sanna-Maria</td><td>18D</td></tr><tr><td>Hongisto, Sanna-Maria</td><td>P-352</td></tr><tr><td>Hooft, Justin</td><td>11B</td></tr><tr><td>Hooiveld, Guido</td><td>P-210</td></tr><tr><td>Hooshmand, Kourosh</td><td>P-443*</td></tr><tr><td>Horibe, Yamato</td><td>P-405</td></tr><tr><td>Horn, Heike</td><td>P-276</td></tr><tr><td>Houpt, Eric</td><td>P-139</td></tr><tr><td>Houthuijs, Kas</td><td>11A</td></tr><tr><td>Houthuijs, Kas</td><td>P-576*</td></tr><tr><td>Houtkooper, Riekelt, H.</td><td>P-180</td></tr><tr><td>Hovinen, Topi</td><td>24B</td></tr><tr><td>Howard, Timothy</td><td>9B</td></tr><tr><td>Howes, Oliver</td><td>P-94</td></tr><tr><td>Hrdinová, Františka</td><td>P-320</td></tr><tr><td>Hrstka, Roman</td><td>13A</td></tr><tr><td>Hsiao, Jordy, J.</td><td>P-574</td></tr><tr><td>Hsieh, Hsieh</td><td>P-595</td></tr><tr><td>Hsu, Ping-Ching</td><td>P-600</td></tr></tbody></table>

<table><thead><tr><th>Name Last, First</th><th>Abstracts</th></tr></thead><tbody><tr><td colspan="2">* = Presenting Author</td></tr><tr><td>Hsu, Wei-Hsiang</td><td>P-88*</td></tr><tr><td>Hsu, Yu-Han</td><td>5D*</td></tr><tr><td>Hu, Chunxiu</td><td>24D</td></tr><tr><td>Hu, Junmin</td><td>P-280</td></tr><tr><td>Hu, Min</td><td>P-319</td></tr><tr><td>Hu, Xin</td><td>P-2</td></tr><tr><td>Hu, Zeping</td><td>21A*</td></tr><tr><td>Hua, Zhang</td><td>P-239</td></tr><tr><td>Huang, Jialing</td><td>P-158*</td></tr><tr><td>Huang, Jiaqi</td><td>P-39</td></tr><tr><td>Huang, Mengna</td><td>25A</td></tr><tr><td>Huang, Mengna</td><td>25C*</td></tr><tr><td>Huang, Mengna</td><td>P-506</td></tr><tr><td>Huber, Caroline</td><td>P-490</td></tr><tr><td>Huber, Florian</td><td>P-538*</td></tr><tr><td>Huerta, José, María</td><td>P-227</td></tr><tr><td>Huerta, Rangel</td><td>P-232</td></tr><tr><td>Hughes, Chris</td><td>P-583</td></tr><tr><td>Hughes, David</td><td>P-31</td></tr><tr><td>Hughes, Juliette</td><td>5C</td></tr><tr><td>Huhmer, Andreas</td><td>P-277</td></tr><tr><td>Huhmer, Andreas</td><td>P-410</td></tr><tr><td>Huhmer, Andreas</td><td>P-554</td></tr><tr><td>Hui, Zhang</td><td>P-416</td></tr><tr><td>Huigen, Marleen, CDG</td><td>P-265</td></tr><tr><td>Huina, Zhou</td><td>P-416</td></tr><tr><td>Hummer, Joanna</td><td>P-185</td></tr><tr><td>Hung, Cheng-Yu</td><td>P-595*</td></tr><tr><td>Hunter, Charles</td><td>P-438</td></tr><tr><td>Hunter, Christie</td><td>P-587</td></tr><tr><td>Husain, Deeba</td><td>P-7*</td></tr><tr><td>Husson, Jwanro</td><td>2D</td></tr><tr><td>Huth, Cornelia</td><td>P-158</td></tr><tr><td>Huttenhower, Curtis</td><td>28A</td></tr><tr><td>Huybrechts, Inge</td><td>P-123</td></tr><tr><td>Huynh, Kevin</td><td>13B</td></tr><tr><td>Huyser, Johan</td><td>14C</td></tr><tr><td>Huysman, Steve</td><td>P-307*</td></tr><tr><td>Huysman, Steve</td><td>P-98</td></tr><tr><td>Hvinden, Ingvild, Comfort</td><td>P-41*</td></tr><tr><td>Hwang, Geum-Sook</td><td>P-113</td></tr><tr><td>Hwang, Geum-Sook</td><td>P-186</td></tr><tr><td>Hwang, Geum-Sook</td><td>P-192</td></tr><tr><td>Hyeon, Hyejin</td><td>P-333*</td></tr><tr><td>Hyeon, Hyejin</td><td>P-363</td></tr><tr><td>Hyka-Nouspikel, Nevila</td><td>19A</td></tr></tbody></table>

<table><thead><tr><th>Name Last, First</th><th>Abstracts</th></tr></thead><tbody><tr><td colspan="2">* = Presenting Author</td></tr><tr><td>Hyöty, Heikki</td><td>P-150</td></tr><tr><td>Hyötyläinen, Tuulia</td><td>27C</td></tr><tr><td>Hyötyläinen, Tuulia</td><td>P-301*</td></tr><tr><td>Hyötyläinen, Tuulia</td><td>P-94</td></tr><tr><td>Hyötyläinen, Tuulia</td><td>P-150</td></tr><tr><td>Hyötyläinen, Tuulia</td><td>P-157</td></tr><tr><td>Hyötyläinen, Tuulia</td><td>P-278</td></tr><tr><td>Hyötyläinen, Tuulia</td><td>P-314</td></tr><tr><td>Iannuccelli, Nathalie</td><td>P-238</td></tr><tr><td>Iau, Philip</td><td>P-143</td></tr><tr><td>Ibañez, Alfredo, J.</td><td>P-362</td></tr><tr><td>Idaghdour, Youssef</td><td>P-146</td></tr><tr><td>Igel, Christian</td><td>P-528</td></tr><tr><td>Iida, Junko</td><td>P-200</td></tr><tr><td>Iida, Miho</td><td>P-100</td></tr><tr><td>Iida, Miho</td><td>P-183</td></tr><tr><td>Iijima, Yoko</td><td>P-331*</td></tr><tr><td>Ikeda, Kaori</td><td>P-356</td></tr><tr><td>Ikeda, Kaori</td><td>P-366</td></tr><tr><td>Ikeda-Ohtsubo, Wakako</td><td>P-381</td></tr><tr><td>Ikonen, Elina</td><td>P-120</td></tr><tr><td>Ikram, Arfan</td><td>9B</td></tr><tr><td>Ikram, Mohammad, Arfan</td><td>P-475</td></tr><tr><td>Ilonen, Jorma</td><td>P-150</td></tr><tr><td>Ilonen, Jorma</td><td>P-301</td></tr><tr><td>Im, Jueng, Eun</td><td>P-144</td></tr><tr><td>Imai, Aina</td><td>P-340</td></tr><tr><td>Imhof, Axel</td><td>28B</td></tr><tr><td>Inagaki, Hikaru</td><td>P-331</td></tr><tr><td>Inagaki, Nobuya</td><td>P-356</td></tr><tr><td>Inagaki, Nobuya</td><td>P-366</td></tr><tr><td>Inglese, Paolo</td><td>P-59</td></tr><tr><td>Inoue, Jin</td><td>P-105</td></tr><tr><td>Inoue, Manami</td><td>P-509</td></tr><tr><td>Ioannidi, Maria-Konstantina</td><td>P-177*</td></tr><tr><td>Ipsen, Yuguang</td><td>10D</td></tr><tr><td>Irifune, Tomoya</td><td>P-364</td></tr><tr><td>Isaac, Giorgis</td><td>P-254*</td></tr><tr><td>Ishibashi, Megumi</td><td>P-212</td></tr><tr><td>Ishihara, Junko</td><td>P-509</td></tr><tr><td>Iskandar, Anita</td><td>P-312</td></tr><tr><td>Iso, Hiroyasu</td><td>P-509</td></tr><tr><td>Isomura, Nozomi</td><td>P-366*</td></tr><tr><td>Isomura, Nozomi</td><td>P-356</td></tr><tr><td>István, Gyertyán</td><td>P-91</td></tr></tbody></table>

<table><thead><tr><th>Name Last, First</th><th>Abstracts</th></tr></thead><tbody><tr><td colspan="2">* = Presenting Author</td></tr><tr><td>Itkin, Maxim</td><td>P-546</td></tr><tr><td>Ito, Satoshi</td><td>P-295</td></tr><tr><td>Ito, Takahiro</td><td>P-264</td></tr><tr><td>Ito, Yusuke</td><td>P-331</td></tr><tr><td>Ivanisevic, Julijana</td><td>P-14*</td></tr><tr><td>Ivanov, N.V.</td><td>P-529</td></tr><tr><td>Ivanov, N.V.</td><td>P-565</td></tr><tr><td>Ivanova, Lada</td><td>P-232*</td></tr><tr><td>Iwanowa, Xenia</td><td>P-206</td></tr><tr><td>Iwanowa, Xenia</td><td>P-573</td></tr><tr><td>Iwasaki, Motoki</td><td>P-509</td></tr><tr><td>Iwata, Takeshi</td><td>P-494</td></tr><tr><td>Iyer, Gayatri</td><td>15D</td></tr><tr><td>Iyer, Gayatri</td><td>P-495</td></tr><tr><td>Izumi, Takashi</td><td>P-251</td></tr><tr><td>Izumi, Yoshihiro</td><td>P-578</td></tr><tr><td>J, Bierau</td><td>P-131</td></tr><tr><td>J, Griffin</td><td>P-179</td></tr><tr><td>J, Habets, D, D</td><td>P-131</td></tr><tr><td>J, Hietala</td><td>P-95</td></tr><tr><td>J, Suvisaari</td><td>P-95</td></tr><tr><td>J, Kokot, Zenon</td><td>P-225</td></tr><tr><td>Jabłońska, Patrycja</td><td>P-175</td></tr><tr><td>Jacobs, Denise</td><td>P-516</td></tr><tr><td>Jacobs, Doris</td><td>18B*</td></tr><tr><td>Jacobs, Doris</td><td>P-338</td></tr><tr><td>Jacobsen, Suganya</td><td>P-174</td></tr><tr><td>Jáčová, Jaroslava</td><td>P-164</td></tr><tr><td>Jacyna, Julia</td><td>P-500*</td></tr><tr><td>Jacyna, Julia</td><td>P-44</td></tr><tr><td>Jaddoe, Vincent, W.V.</td><td>P-249</td></tr><tr><td>Jahn, Petr</td><td>P-320</td></tr><tr><td>Jain, Abhishek</td><td>4B</td></tr><tr><td>Jain, ShreyansK.</td><td>P-220</td></tr><tr><td>Jan, Matysiak</td><td>P-225</td></tr><tr><td>Janáky, Tamás</td><td>P-90</td></tr><tr><td>Janečková, Hana</td><td>P-320</td></tr><tr><td>Jang, Hwan-hee</td><td>P-341*</td></tr><tr><td>Jang, In-Cheol</td><td>P-426*</td></tr><tr><td>Jang, In-Jin</td><td>P-216</td></tr><tr><td>Jang, Ki-Chang</td><td>P-299</td></tr><tr><td>Jang, Ki-Chang</td><td>P-342</td></tr><tr><td>Jang, Kyoung-Soon</td><td>P-319*</td></tr><tr><td>Jang, Kyoung-Soon</td><td>P-380</td></tr><tr><td>Jang, Seoyoung</td><td>P-113*</td></tr><tr><td>Jang, Won-Jun</td><td>P-70</td></tr><tr><td>Jankevics, Andris</td><td>P-107</td></tr><tr><td>Jankevics, Andris</td><td>P-581</td></tr></tbody></table>