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Sep 25, 2019

Version 2

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# Preparation of LB Media V.2

DOI

https://dx.doi.org/10.17504/protocols.io.7pdhmi6

NUS iGEM¹

¹National University of Singapore

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NUS iGEM

National University of Singapore

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DOI: https://dx.doi.org/10.17504/protocols.io.7pdhmi6

**Protocol Citation:** NUS iGEM 2019. Preparation of LB Media. protocols.io https://dx.doi.org/10.17504/protocols.io.7pdhmi6

**License:** This is an open access protocol distributed under the terms of the **Creative Commons Attribution License**, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

**Protocol status:** Working

We use this protocol and it's working

Created: September 25, 2019
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Last Modified: September 12, 2023

Protocol Integer ID: 28101

**Keywords:** preparation

# Materials

## MATERIALS

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Water refers to sterilized deionized water

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Luria Broth Base (Miller's LB Broth Base)&trade;, powder Thermo Fisher Catalog #12795027

## STEP MATERIALS

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Luria Broth Base (Miller's LB Broth Base)&trade;, powder Thermo Fisher Catalog #12795027

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Water refers to sterilized deionized water

# Protocol materials

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Water refers to sterilized deionized water

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Luria Broth Base (Miller's LB Broth Base)&trade;, powder Thermo Fisher Catalog #12795027

# Troubleshooting
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1. Weigh **25 g** of Luria Broth Base powder.

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Luria Broth Base (Miller's LB Broth Base)&trade;, powder **Thermo Fisher Catalog #12795027**

2. Add the powder into **1 L** of water.

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Water refers to sterilized deionized water

3. Autoclave entire bottle of LB media.