# Pepsin localization in digested meat by ToF-SIMS analysis

Thierry Astruc, Annie Venien, Olivier Loison, Delphine Pavon

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Thierry Astruc, Annie Venien, Olivier Loison, Delphine Pavon. Pepsin localization in digested meat by ToF-SIMS analysis. International Symposium on Food Nutrition and Health, May 2017, Dalian, China. 2017. [hal-01594929](https://hal.science/hal-01594929)

HAL Id: hal-01594929

https://hal.science/hal-01594929

Submitted on 26 Sep 2017

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# Pepsin localization in digested meat :
a ToF-SIMS analysis

Thierry Astruc<fnref n="1" />, Annie Vénien<fnref n="1" />, Olivier Loison<fnref n="1" />, Michiyo Motoyama<fnref n="1" />,<fnref n="2" />, Delphine Pavon<fnref n="3" />

<figure><img src="image_2.png" /></figure>

## Context and objectives

<figure><img src="image_3.png" /></figure>

<figure><img src="image_4.png" /></figure>

**Aim : localize pepsin in a digested meat sample**

## Conclusions

* Analysis in ToF SIMS allows the mapping of characteristic nitrogen chemical species of pepsin.

* The pepsin diffuses mainly on a homogeneous front with several transfers through perimysium sheaths.

* Chlorine, mostly coming from HCl of digestive juice, shows a similar diffusion profile than pepsin.

## Material and Methods

<figure><img src="image_5.png" /></figure>

Bovine muscle
15X15X20 mm

* 37°C

* 2 hours

* solution of pepsin 125U/mg of protein.

In vitro pepsin digestion

<figure><img src="image_6.png" /></figure>

10 µm cryosections

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Pepsin powder

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Mass spectroscopy
mapping

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ToF-SIMS V (IONT-TOF GmbH, Munster, Germany)

Bismuth gun - 25 kV (source Bi₂)

<figure><img src="image_10.png" /></figure>

Science et Surface laboratory, Ecully, France

## Results

<figure><img src="image_11.png" /><figcaption>### Nitrogen species CxHyN characteristics of pepsin</figcaption></figure>

<figure><img src="image_12.png" /></figure>

<figure><img src="image_13.png" /><figcaption>### Chlorine</figcaption></figure>

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<figure><img src="image_15.png" /></figure>

Histology,
Yellow: muscle cells
Red: connective tissue (perimysium)

<figure><img src="image_16.png" /></figure>

<footnote n="1">¹INRA - UR370 Qualité des Produits Animaux, Saint-Genès Champanelle, France</footnote>

<footnote n="2">²Institute of Livestock and Grassland Science, National Agriculture and Food Research Organization (NARO), Japan</footnote>

<footnote n="3">³Science et Surface, Ecully, France</footnote>