<figure><img src="image_1.png" /></figure>

HAL
open science

# The Function and Molecular Mechanism of Cellular Senescence-Inhibited Gene

Liwei Ma, Feng Zhu, Nan Xie, Feng Tian, Ying Sun, Tanjun Tong

► To cite this version:

Liwei Ma, Feng Zhu, Nan Xie, Feng Tian, Ying Sun, et al.. The Function and Molecular Mechanism of Cellular Senescence-Inhibited Gene. Cellular & Molecular Medicine: Open access, 2016, The Function and Molecular Mechanism of Cellular Senescence-Inhibited Gene, 2 (2). hal-04036819

HAL Id: hal-04036819

https://hal.science/hal-04036819

Submitted on 20 Mar 2023

HAL is a multi-disciplinary open access
archive for the deposit and dissemination of sci-
entific research documents, whether they are pub-
lished or not. The documents may come from
teaching and research institutions in France or
abroad, or from public or private research centers.

L'archive ouverte pluridisciplinaire HAL, est
destinée au dépôt et à la diffusion de documents
scientifiques de niveau recherche, publiés ou non,
émanant des établissements d'enseignement et de
recherche français ou étrangers, des laboratoires
publics ou privés.

<figure><img src="image_2.png" /></figure>

Distributed under a Creative Commons Attribution 4.0 International License
# The Function and Molecular Mechanism of Cellular Senescence-Inhibited Gene

Liwei Ma, Feng Zhu, Nan Xie, Feng Tian, Ying Sun and Tanjun Tong*

Research Center on Aging, Department of Biochemistry and Molecular Biology, Peking University Health Science Center, Beijing, 100191, P.R. China

*Corresponding author: Tanjun Tong, Department of Biochemistry and Molecular Biology, Peking University Health Science Center, 38 Xueyuan Road, Beijing 100191, P.R. China, Tel: + 8610-82801454; Fax: + 8610-82802931; E-mail: tztong@bjmu.edu.cn

Rec date: May 06, 2016; Acc date: May 27, 2016; Pub date: May 29, 2016

Copyright: © 2016 Ma L, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Citation: Ma L, Zhu F, Xie N, et al. The Function and Molecular Mechanism of CSIG. Cell Mol Med. 2016, 2:2.

## Abstract

To explore the genetic mechanism of replicative senescence, we identified and cloned a Cellular Senescence-Inhibited Gene/ribosomal L1 domain containing 1 (CSIG/RSL1D1) through suppressive subtractive hybridization. CSIG expression is high in early-passaged human diploid fibroblast cells but decreased during senescence. Our results demonstrated that CSIG inhibited the senescence progression and mediated the apoptosis signaling pathway under ultraviolet (UV) irradiation. Moreover, emerging evidences have indicated that CSIG is involved in other processes including cell cycle regulation and tumor development. This is a brief overview to introduce the studies on the function and molecular mechanism of CSIG.

**Keywords:** CSIG/RSL1D1; Cellular senescence; Proliferation; Apoptosis

## Introduction

Replicative senescence, a cellular self-protection mechanism to prevent tumor development, contributes to the generation of age-related disorders [1-3]. As the expression alteration of important genes is the molecular basis of replicative senescence, it is necessary to explore the gene expression and signal transduction mechanism during senescence. By analyzing the gene expression profiles in early-passaged and senescent fibroblasts, Guo et al. cloned and characterized Cellular Senescence-Inhibited Gene [CSIG, GenBank accession No. AY154473, also named as ribosomal L1 domain containing 1 (RSL1D1) according to http://www.ncbi.nlm.nih.gov/ through suppression subtractive hybridization in human diploid fibroblasts [4]. CSIG expression is high in early-passaged human diploid fibroblast cells but decreased during senescence [4,5]. Tsai et al. reported that the decreased proliferation of aging tenocytes is associated with the down-regulation of CSIG [6]. The experiments *in vivo* and *in vitro* both indicated that CSIG is not only associated with cellular senescence but also participates in organismal aging [4]. And our previous work proved that CSIG delays cellular

senescence by inhibiting the phosphatase and tensin homolog (PTEN)/p27Kip1 pathway [5].

In addition to the cellular senescence, CSIG is also involved in many other important biological processes. For example, under ultraviolet (UV) irradiation, CSIG mediates the apoptosis signaling pathway [7]. In the process of bone healing and growth, CSIG expression is increased [8], indicating the participation of CSIG in the bone tissue formation, reabsorption, regeneration and healing. Inflammations such as respiratory tract inflammation are accompanied with increased CSIG gene expression [9]. Cheng et al. reported that CSIG facilitated the proliferation of hepatocellular carcinoma [10]. By analysis of dog osteosarcoma samples, Selvarajah et al. found that CSIG was not only related to tumor cells survival but also associated with cell proliferation and cancer metastasis [11], and therefore CSIG may act as a new therapeutic target. Furthermore, Klein et al. found that CSIG expression in breast cancer brain metastases samples was significantly higher than in bone metastases [12]. Thus CSIG may be used as a predictive marker for specific tissue metastasis of breast cancer and offers the possibility of personalized treatment. In addition, Fujiyama-Nakamura et al. found that CSIG was involved in the ribosomal protein synthesis. They detected the interaction of CSIG with Parvulin, a peptidyl-prolyl cis-trans isomerase, in the process of ribosomal protein synthesis [13].

By microarray analysis, we recently discovered that the downstream genes modulated by CSIG are mainly implicated in the processes of cell cycle, apoptosis, transcriptional control, bone formation and cell adhesion [14], which further provides the molecular basis for CSIG to participate in these biological processes.

## Identification and characteristics of CSIG

By comparing the expression profiles of early-passaged human fibroblast cells with that of senescent cells, we previously identified CSIG/RSL1D1 through suppressive subtractive hybridization method [4]. CSIG is abundant in early-passaged fibroblast cells while declines during senescence [4,5]. By localizing on chromosome 16p13m, the human CSIG gene spans 5,137 bp, contains 9 exons and encodes 490 amino acids residues. According to bioinformatics
analysis, CSIG encodes a short half-life alkaline protein with the molecular weight of 54.7 kDa and the isoelectric point (PI) of 10.09. We observed that CSIG was widely expressed in human most tissues with higher expression in heart, skeletal muscle and placental tissues [5]. And the immunofluorescence analysis showed that CSIG protein was predominantly distributed in the nucleolus [5].

As a member of the ribosomal protein L1p family, CSIG is evolutionarily conserved according to the analysis of bioinformatics (http://www.expasy.org). There is a ribosomal protein L1-like domain (residues 31-264) in the CSIG protein N-terminus, indicating that CSIG may be involved in ribosome biosynthesis. As one of the largest ribosomal proteins, ribosomal L1P from the archaea binds directly to 23S rRNA. The L1 stalk is quite mobile in the ribosome, and it is involved in E site tRNA release. In bacteria and archaea, L1 acts as an rRNA-binding ribosomal protein and a translation repressor by binding its own mRNA [15]. The RNA-binding site of L1 is highly conserved, with both mRNA and rRNA binding the same binding site [16]. Like several other large ribosomal subunit proteins, L1 displays RNA chaperone activity [17]. Human CSIG protein C-terminus consists of a long lysine (Lys)-rich region (residues 282-485) similar to many ribosomal proteins including L14, L21, L22, L23, L24, L29 and L35. Our previous results proved that CSIG C-terminal Lys-rich region interacted with the inhibitor of growth family member 1 (ING1, also as p33ING1) [6]. Meng et al. reported that the C-terminal (residues 317-452) part of mouse homolog RSL1D1 without the L1 domain bound to nucleostemin [18]. These evidences suggested a role of protein binding activity for CSIG C-terminus.

## CSIG and senescence

As described above, CSIG is abundant in early-passaged fibroblast cells, but declines in senescent cells. Consistently, in human peripheral blood leukocytes, we observed the same expression pattern of CSIG with age (CSIG level is significantly higher in the samples from the newborns while lower in that from old peoples) [4], which suggested that CSIG might be implicated in aging *in vitro* and *in vivo*. Coincidentally, Chiocchetti et al. reported that yeast protein RPL10, which shares a similar domain with CSIG, could affect the life span of yeast [19].

The human diploid fibroblast cells are generally served as the established model of replicative senescence. Just using this model, we firstly explore the function of CSIG during senescence and the possible mechanism. Through functional studies we found that CSIG could significantly affect the cell cycle and inhibit the cellular senescence progression [5]. CSIG overexpression increased the percentage of S phase cells, promoted cell proliferation, reduced the senescence-associated β-galactosidase (SA-β-gal) activity, and prolonged the cell lifespan by more than 10 population doublings (PDs) [5]. Conversely, CSIG silencing increased the percentage of G1 phase cells, inhibited cell growth, increased SA-β-gal activity, and shortened the cell lifespan by accelerating cell entry into the stable G1 phase arrest status (named as senescence) [5].

The results suggested that the high level of CSIG contributed to maintain the active state of early-passage cells, while the decline of CSIG expression during senescence progression might be well causally implicated in generating senescence phenotype.

Our further study showed that CSIG delayed cellular senescence through down-regulating PTEN/p27Kip1 pathway [5]. During senescence, CSIG expression declines and its inhibition on tumor suppressor gene PTEN and cyclin-dependent kinase inhibitor p27Kip1 diminishes accordingly. Then the accumulated PTEN in turn leads to the cell cycle G1 phase arrest through increasing the level of p27Kip1. Our results also indicated that CSIG could inhibit the synthesis of PTEN nascent protein and PTEN was a necessary factor for CSIG to modulate cellular senescence [5]. Totally, our findings suggest that CSIG plays an inhibitory role during cellular senescence and PTEN acts as an essential mediator for CSIG to regulate senescence [5]. The cell aging-inhibited effect of CSIG is coincided well with the experiment results from other system. Mouse CSIG/RSL1D1 was reported to regulate the activity of nucleostemin through modulating its subcellular distribution (in the nucleolus or nucleoplasm) [18]. And nucleostemin was proved to delay the senescence progression in the mice fibroblasts [20]. Thus in the mice fibroblasts, CSIG/RSL1D1 probably controls the cell aging progression by regulating the activity of nucleostemin (Figure 1).

To study the domain importance of CSIG to regulate cell proliferation and senescence, we performed the domain analysis. The results showed that the ribosomal L1 domain and Lys-rich region were both essential for CSIG to modulate cell proliferation and cellular senescence [21]. We further explored the regulatory mechanism of CSIG expression during senescence. We found that the E2F transcription factor 1 (E2F1) up-regulated CSIG expression by binding and activating CSIG promoter in early-passage cells [22]. While E2F1 expression level and its transcriptional activation effect declined during senescence, which in turn led to the decrease of CSIG expression [22].

## CSIG and apoptosis

Mediating the apoptosis pathway under stress is another previously unknown non-ribosomal function for CSIG. Following cell exposure to UV irradiation, p33ING1, a member of inhibitor of growth family member 1 (ING1) family, was accumulated and transferred from nucleus to nucleolus where CSIG bound to and interacted with p33ING1 [6]. CSIG was also induced under the DNA damage stress and influenced the retention time of p33ING1 in nucleolus [6]. By sequestered in nucleolus by CSIG, the nucleolus p33ING1 further triggered apoptosis through activating downstream key molecules such as BCL2 associated X protein (Bax) [6]. Therefore, CSIG is indispensable for p33ING1 to induce apoptosis in response to UV irradiation.

It is worth mentioning that ribosomal RNA processing 1 homolog B (RRP1B), an interactive protein with CSIG, is reported to regulate E2F1-induced apoptosis [23,24]. Following DNA damage, E2F1 expression increased and firstly
activated the transcription of RRP1B, and then RRP1B assisted E2F1 to transcriptionally activate more key molecules to induce apoptosis [24]. As E2F1 regulates other target genes through RRP1B assistance, RRP1B is necessary for E2F1 to induce apoptosis. As the transcriptional targets of E2F1, both CSIG and RRP1B participate in apoptosis process (Figure 1). Although we have known that CSIG and RRP1B promote

apoptosis through different pathways, it is still possible that CSIG might interact and cooperate with RRP1B during the process of apoptosis induction through an unknown mechanism so far. And whether CSIG really involves in the E2F1-induced apoptosis needs to be further confirmed (Figure 1).

<figure><img src="image_3.png" /></figure>

## Perspective

CSIG plays a critical role in not only cellular senescent, but also other processes including cell apoptosis, cell cycle regulation and tumor development (Figure 1). However, many aspects about the function of CSIG and the underlying mechanism still need to be further investigated. Given that cellular senescence is a complicated physiological process regulated by multiple genes and pathways, whether other cell cycle-related proteins and pathways besides PTEN/p27Kip1 are also involved in mediating the influence of CSIG on senescence is worth exploring. Our recent results indicated that cell

division cycle 14B (Cdc14B), one of CSIG-regulated genes, is really involved in senescence regulation (unpublished). The analysis of samples from human and mouse suggested that CSIG might well participate in the progression of cell aging in vivo. Therefore, we are constructing a CSIG/RSL1D1 knock-out mouse model to further explore the function and molecular mechanism of CSIG in tissue aging. By confirming the results in vitro, a more solid and comprehensive view on CSIG will be provided for better understanding its roles and the molecular mechanisms which drive its action. On the other hand, further investigation into the fundamental roles of CSIG such as ribosome-associated function will help us to understand the
post-transcriptional or translational regulation mechanism of CSIG. Totally, all these studies on CSIG function and the mechanism will contribute to better understanding of the molecular mechanism of cell aging, apoptosis and tumor development, which will in turn provide the experimental basis for the treatment of aging-related disorders and cancer.

## Acknowledgments

This work was supported by the National Basic Research Programs of China (Grant No. 2013CB530801).

## References

1. Laberge RM, Sun Y, Orjalo AV, Patil CK, Freund A, et al. (2015) MTOR regulates the pro-tumorigenic senescence-associated secretory phenotype by promoting IL1A translation, NAT CELL BIOL 17: 1049-1061.

2. Campisi J, Robert L (2014) Cell senescence: role in aging and age-related diseases, Interdiscip Top Gerontol 39: 45-61.

3. Campisi J (2013) Aging, cellular senescence, and cancer, Annu Rev Physiol 75: 685-705.

4. Guo SZ, Zhang ZY, Tong TJ (2004) Cloning and characterization of cellular senescence-associated genes in human fibroblasts by suppression subtractive hybridization., Exp Cell Res 298: 465-472.

5. Ma LW, Chang N, Guo SZ Li Q, Zhang Z, et al. (2008) CSIG Inhibits PTEN Translation in Replicative Senescence. Mol Cell Biol. 28: 6290-6301.

6. Tsai WC, Chang HN, Yu TY, Chien CH, Fu LF, et al. (2011) Decreased proliferation of aging tenocytes is associated with down-regulation of cellular senescence-inhibited gene and up-regulation of p27, J Orthop Res 29: 1598-1603.

7. Li N, Zhao G, Chen T, Xue L, Ma L, et al. (2012) Nucleolar protein CSIG is required for p33ING1 function in UV-induced apoptosis, CELL DEATH DIS 3 (2012)e283.

8. Li N, Zhao G, Chen T, Xue L, Ma L, et al. (2011) Transcriptome analysis of beta-TCP implanted in dog mandible. Bone 48: 864-877.

9. Misior AM, Deshpande DA, Loza MJ, Pascual RM, Hipp JD, et al. (2009) Glucocorticoid- and protein kinase A-dependent transcriptome regulation in airway smooth muscle. Am J Resp Cell Mol 41: 24-39.

10. Cheng Q, Yuan F, Lu F, Zhang B, Chen T, et al. (2015) CSIG promotes hepatocellular carcinoma proliferation by activating c-MYC expression, Oncotarget 6: 4733-4744.

11. Selvarajah GT, Kirpensteijn J, van Wolferen ME, Rao NA, Fieten H, et al. (2009) Gene expression profiling of canine osteosarcoma reveals genes associated with short and long survival times, Mol Cancer 8: 72.

12. Klein A, Olendrowitz C, Schmutzler R, Hampl J, Schlag PM, et al. (2009) Identification of brain- and bone-specific breast cancer metastasis genes. Cancer Lett 276: 212-220.

13. Fujiyama-Nakamura S, Yoshikawa H, Homma K, Hayano T, Tsujimura-Takahashi T, et al. (2009) a peptidyl-prolyl cis-trans isomerase, is a novel rRNA processing factor that evolved in the metazoan lineage. Mol Cell Proteomics 8: 1552-1565.

14. Ma L, Zhao W, Zhu F, Yuan F, Xie N, et al. (2015) Global characteristics of CSIG-associated gene expression changes in human HEK293 Cells and the implications for CSIG regulating cell proliferation and senescence. Front Endocrinol (Lausanne) 6: 69.

15. Nevskaya N, Tishchenko S, Volchkov S, Kljashtorny V, Nikonova E, et al. (2006) New insights into the interaction of ribosomal protein L1 with RNA. J Mol Biol 355: 747-759.

16. Nevskaya N, Tishchenko S, Gabdoulkhakov A, Nikonova E, Nikonov O, et al. (2005) Ribosomal protein L1 recognizes the same specific structural motif in its target sites on the autoregulatory mRNA and 23S rRNA, Nucleic Acids Res 33: 478-485.

17. Ameres SL, Shcherbakov D, Nikonova E, Piendl W, Schroeder R, et al. (2007) RNA chaperone activity of L1 ribosomal proteins: phylogenetic conservation and splicing inhibition. Nucleic Acids Res 35: 3752-3763.

18. Meng L, Yasumoto H, Tsai RY (2006) Multiple controls regulate nucleostemin partitioning between nucleolus and nucleoplasm. J Cell Sci 119: 5124-5136.

19. Chiocchetti A, Zhou J, Zhu H, Karl T, Haubenreisser O, et al. (2007) Ribosomal proteins Rpl10 and Rps6 are potent regulators of yeast replicative life span. Exp Gerontol 42: 275-286.

20. Zhu Q, Yasumoto H, Tsai RY (2006) Nucleostemin delays cellular senescence and negatively regulates TRF1 protein stability. Mol Cell Biol 26: 9279-9290.

21. Ma L, Zhao W, Zheng Q, Chen T, Qi J, et al. (2016) Ribosomal L1 domain and lysine-rich region are essential for CSIG/ RSL1D1 to regulate proliferation and senescence. Biochem Biophys Res Commun 469: 593-598.

22. Ma L, Zhao W, Zheng Q, Chen T, Qi J, et al. (2012) The retinoblastoma protein selectively represses E2F1 targets via a TAAC DNA element during cellular senescence. J Biol Chem 287: 37540-3751.

23. Crawford NP, Yang H, Mattaini KR, Hunter KW (2009) The metastasis efficiency modifier ribosomal RNA processing 1 homolog B (RRP1B) is a chromatin-associated factor. J Biol Chem 284: 28660-2873.

24. Paik JC, Wang B, Liu K, Lue JK, Lin WC, et al. (2010) Regulation of E2F1-induced apoptosis by the nucleolar protein RRP1B. J Biol Chem 285: 6348-6363.