# Contribution of multispectral autofluorescence imaging to histochemistry in understanding sorghum internode hydrolysis pattern?

Cécile Barron, Juliette Auverlot, David Pot, Marie-Françoise Devaux, Sylvie Jaffuel

► To cite this version:

Cécile Barron, Juliette Auverlot, David Pot, Marie-Françoise Devaux, Sylvie Jaffuel. Contribution of multispectral autofluorescence imaging to histochemistry in understanding sorghum internode hydrolysis pattern?. Sorghum in the 21st Century, Global Sorghum Conference, CIRAD; Kansas State University; Collaborative Research on Sorghum and Millet; SorghumID; IRD; CERAAS, Jun 2023, Montpellier, France. pp.317. [hal-05181925](https://hal.science/hal-05181925v1)

HAL Id: hal-05181925

https://hal.science/hal-05181925v1

Submitted on 23 Jul 2025

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# Sorghum in the 21st Century
## Global Sorghum Conference

Resiliency and Sustainability in the Face of Climate Change

June 5-9 2023

The Corum Event Center, Montpellier, France

Lead Sponsor

KANSAS STATE
UNIVERSITY.

College of Agriculture

Supporting the

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INTERNATIONAL YEAR OF
MILLETS
2023

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## BOOK OF ABSTRACTS

PROGRAM - ABSTRACTS

List of posters - List of participants

List of exhibitors - Exhibition plan

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cirad

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Sorghum
Institut d'Études
Raisons et Développements
FRANCE

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CERAMS
Centre d'Étude régional
de rédaction à la sauveté
# Contribution of multispectral autofluorescence imaging to histochemistry in understanding sorghum internode hydrolysis pattern ?

Barron Cecile<fnref n="1" /> (cecile.barron@inrae.fr), Juliette Auverlot², David Pot³, Marie France Devaux⁴, Sylvie Jaffuel²

Digestibility of plant feedstock is one of the key properties and is largely dependent on the overall lignification of the plant which is monitored at the tissue scale by the relative amounts and composition of plant organ. To obtain details on anatomy and spatial distribution of main components high-throughput histochemical methods have been developed on maize and sorghum internodes [1,2]. FASGA staining procedure is based on the competition between 2 dyes and reveals regions with differentiated in terms of tissue lignification [3]. In addition to staining procedures, the specific spectral properties of phenolic compounds could also be exploited to map their distributions using spectral imaging technics. Multispectral autofluorescence imaging was successfully applied to maize stem internode to reveal differences in cell wall phenolic compounds distribution (lignin and hydroxycinnamic acids) [4]. The objective of this work was to evaluate the relevance of multispectral autofluorescence imaging to better understand contrasted hydrolysis patterns observed inside the sorghum stem internode.

## References:

1. Perrier et al. Front. Plant Sci, 2017, 1516.

2. Tolivia et Tolivia, J. Microscopy Oxford. 1987, 113-117.

3. Berger et al. Front. Plant Science, 2021, 12.

<footnote n="1">¹ UMR 1208 IATE, INRAE, Montpellier, France ; ² Phenomen/AGAP/BIOS, Cirad, Montpellier, France ; ³ GIV/AGAP/BIOS, Cirad, Montpellier, France ; ⁴ BIA, INRAE UR 1268, Nantes, France</footnote>