# The rhizome of life: what about metazoa?

Hemalatha G Ramulu, Didier Raoult, Pierre Pontarotti

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Hemalatha G Ramulu, Didier Raoult, Pierre Pontarotti. The rhizome of life: what about metazoa?. Frontiers in Cellular and Infection Microbiology, 2012, 2, pp.50. 10.3389/fcimb.2012.00050. hal-00831099

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# The rhizome of life: what about metazoa?

Hemalatha G. Ramulu<fnref n="1" />,<fnref n="2" />, Didier Raoult<fnref n="2" /> and Pierre Pontarotti<fnref n="1" />*

**Edited by:**

Eugene V. Koonin, National
Institutes of Health, USA

**Reviewed by:**

Jose A. Bengoechea, Fundacion
Caubet-CIMERA Illes Balears, Spain
Andrey P. Anisimov, State Research
Center for Applied Microbiology
and Biotechnology, Russia

**\*Correspondence:**

Pierre Pontarotti, Evolution
Biologique et Modélisation, Aix
Marseille Université, UMR-CNRS
7353, case 19, 3, Place Victor Hugo,
Marseille cedex 3 13331 France.
e-mail: pierre.pontarotti@univ-
provence.fr

The increase in huge number of genomic sequences in recent years has contributed to various genetic events such as horizontal gene transfer (HGT), gene duplication and hybridization of species. Among them HGT has played an important role in the genome evolution and was believed to occur only in Bacterial and Archaeal genomes. As a result, genomes were found to be chimeric and the evolution of life was represented in different forms such as forests, networks and species evolution was described more like a rhizome, rather than a tree. However, in the last few years, HGT has also been evidenced in other group such as metazoa (for example in root-knot nematodes, bdelloid rotifers and mammals). In addition to HGT, other genetic events such as transfer by retrotransposons and hybridization between more closely related lineages are also well established. Therefore, in the light of such genetic events, whether the evolution of metazoa exists in the form of a tree, network or rhizome is highly questionable and needs to be determined. In the current review, we will focus on the role of HGT, retrotransposons and hybridization in the metazoan evolution.

**Keywords:** tree of life, horizontal gene transfer, retrotransposons, hybridization, metazoa

## PROPOSED CONCEPTS FOR TREE OF LIFE AND POSITION OF METAZOA

Understanding the relationships among all living organisms by phylogenetic tree reconstruction is one of the fundamental challenges in biology. For almost 200 years, Tree of Life (TOL) has been the most powerful metaphors for biologists in depicting the evolutionary history of organisms. One of the first and most explicit form of TOL was presented by German zoologist Ernst Haeckel (1866), but its exact shape has remained elusive. Indeed, several studies to deduce TOL using various methods were carried out (Fox et al., 1980; Doolittle, 1981; Fitz-Gibbon and House, 1999; Snel et al., 1999; Tekaia et al., 1999; Lin and Gerstein, 2000; Brown et al., 2001; Clarke et al., 2002; Korbel et al., 2002; Rokas et al., 2003; Kunin et al., 2005), but its principal existence is heavily debated.

The rapid increase in molecular and genomic data in recent years have contributed to genetic events such as horizontal gene transfer (HGT), that is often considered as a major constraint in the reconstruction of phylogenetic trees. HGT “the non-genealogical transmission of genetic material from one organism to another” (Goldenfeld and Woese, 2007) is an important driving force in genomic evolution. HGT has contributed to early evolution of life to a larger extent than is presently occurring in modern biota (Zillig et al., 1992; Kandler, 1994, 1998; Woese, 2002). Indeed, number of studies have been reported about the genes acquired by HGT in three domains of life, such as Bacteria (Saunders et al., 1999; Ochman et al., 2000), Archaea (Doolittle and Logsdon, 1998; Faguy and Doolittle, 1999) and Eukaryotes (Andersson, 2005) and also between domains, i.e., from Bacteria to Archaea (Gophna et al., 2004), from Archaea to Eukarya (Andersson et al., 2003), from Bacteria to Eukarya (Watkins and

Gray, 2006), from Eukarya to Bacteria (Guljamow et al., 2007) and even within Eukarya (Nedelcu et al., 2008).

Conversely, it has been assumed that the role of HGT is not prevalent in other multicellular eukaryotic organisms like in kingdom Animalia or metazoa. However, the possibility of gene transfers among them have increased in recent years and were reported in various groups such as Porifera (Rot et al., 2006), Cnidaria (Chapman et al., 2010), Nematoda (Danchin et al., 2010), Arthropoda (Fenn et al., 2006; Hotopp et al., 2007), Rotifera (Gladyshev et al., 2008), and Craniata (Graham et al., 2008; Pace et al., 2008). In addition to HGT, other genetic events such as transfer by retrotransposons and hybridization (Seehausen, 2004) were also reported, which are proposed to play an important role in the evolution of metazoa.

The studies discussed above support that HGT has played a significant role in modulating the metazoan evolution including prokaryotes and eukaryotes. Consequently, the increase in the prevalence of HGT events in Bacteria, Archaea, and Eukaryotes have resulted in the chimeric nature of genomes, where different parts of the genome can have different evolutionary histories and its difficult to identify a single common ancestor for the gene repertoire of any organism. All these results have conferred to undermine the TOL concept, thereby giving rise to a new paradigm. As a result, many proposals have emerged for the tree-like pattern replacing it with more complex models such as the “reticulate evolution” (Sneath, 1975), “synthesis of life” (Bapteste et al., 2004), “web of life” (Doolittle, 1999), “ring of life” (Rivera and Lake, 2004), “network of life” (Ragan et al., 2009), the “forest” of evolutionary trees (Puigbo et al., 2009; Schliep et al., 2010), the genetic network (Puigbo et al., 2010; Popa et al., 2011). Moreover, the evolution of species was described more like

<footnote n="1">¹ LATP UMR-CNRS 7353, Evolution Biologique et Modélisation, Aix-Marseille Université, Marseille, France</footnote>

<footnote n="2">² URMITE CNRS-IRD UMR6236-198, Marseille, France</footnote>
a rhizome (Deleuze and Guattari, 1976; Raoult, 2010), reflect-
ing various origins of genomic sequences in each species (Raoult,
2010).

The phylogenetic relationships among the major groups of
animals was represented in the form of a tree (Bergstrom, 1985;
Field et al., 1988; Valentine, 1997; Halanych, 2004; Giribet et al.,
2007; Dunn et al., 2008). However, the increase in the extent of
HGT, retrotransposons, and hybridization events in the metazoan
lineage precludes the reconstruction of animal TOL. In addition
to these, recent studies on whole genomic sequences from various
metazoan phyla suggest a chimeric origin for its major groups due
to the presence of HGT and hybridization (Syvanen and Ducore,
2010).

The results from these studies pose new questions about the
evolution of metazoa, as whether it exists in the form of network
or rhizome of life? Therefore, there is a need for a new pattern to
be determined. In the current review, we will discuss the role of
HGT, transfer by retrotransposons, hybridization and the chal-
lenges that it proposes for the current metazoan evolutionary
paradigm.

METAZOA AND ITS CLASSIFICATION

Metazoa (animals) represent a group of multicellular eukary-
otes (Haeckel, 1874) and constitute a monophyletic clade with
heterotrophic organisms. They are believed to have diversified
around the beginning of the Cambrian period (~543 mil-
lion years ago). According to the recent reports, the meta-
zoan group includes approximately 1.3 million described living
species distributed in 35-40 phyla (subjected to the classification)
(Edgecombe et al., 2011). This exponential growth in molecular
sequence data in the metazoan group has increased the amount
of phylogenetic information to study the animal relationships.

According to the Linnaeus (1758) system of classification,
kingdom Animalia was classified into six classes: Amphibia,
Aves, Mammalia, Pisces, Reptilia, Insecta, and Vermes, the lat-
ter divided into Intestina, Mollusca, Testacea, Lithophyta, and
Zoophyta. Later on this classification has been revised con-
stantly by biologists to study their evolutionary relationships.
The early metazoan tree on animal phylogeny was based on 18S
rRNA (Field et al., 1988) and Cnidaria, Ctenophora, Placozoa,
Porifera, and Bilateria constituted the basal metazoan groups
(Medina et al., 2001; Collins et al., 2005). However, the increase
in molecular data has given rise to so-called “new animal phy-
logeny” (Adoutte et al., 2000; Halanych, 2004; Giribet et al., 2007)
supporting the monophyly of Bilateria, which is again divided
into two major lineages, Protostomia, and Deuterostomia. These
two clades are well resolved by broad taxon sampling (Dunn
et al., 2008; Hejnol et al., 2009; Philippe et al., 2009). The
diversity within Protostomia led to division of two clades,
Ecdysozoa (Aguinaldo et al., 1997; Schmidt-Rhaesa et al., 1998;
Giribet, 2003; Telford et al., 2008), and Spiralia (Spiralia or
Lophotrochozoa; Halanych et al., 1995; Giribet et al., 2000,
2009; Halanych, 2004; Giribet, 2008). Deuterostomia encom-
pass two clades, Ambulacraria (Winchell et al., 2002; Brown
et al., 2008), and Chordata (Delsuc et al., 2006; Mallatt and
Winchell, 2007). The present consensus on metazoan phy-
logeny based on various hypotheses is represented in **Figure 1**.

<figure><img src="image_3.png" /><figcaption>**FIGURE 1** | The current phylogenetic relationships in Metazoa. This tree was generated by consensus based on various phylogenetic studies.</figcaption></figure>

(Figure 1, Edgecombe et al., 2011). The relationships on deep
metazoan groups have been extensively reviewed elsewhere
(Edgecombe et al., 2011).

HORIZONTAL GENE TRANSFER IN METAZOA

HGT in animals has long been neglected and considered to be
rare. However, the increase in molecular data in recent years has
contributed to the possibility of gene transfers in various meta-
zoan phyla such as Porifera, Cnidaria, Nematoda, Arthropoda,
Rotifera, and Craniata, thereby creating the need to analyze more
transfer events in other unidentified metazoan groups. Therefore,
we will discuss the transfer events in each of them in the following
sections respectively.
## TRANSFER IN PHYLUM PORIFERA (SPONGE)

Poriferans represent the earliest diverging metazoans due to the presence of distinct cell types called choanocytes, which are similar to choanoflagellates, the closest unicellular relatives of metazoans (Medina et al., 2001; Nielsen, 2001; Muller, 2003). Other unique features include the lack of intestinal epithelium and digestive parenchyma (Ereskovsky and Dondua, 2006). The molecular analyses resolved sponges at the base of metazoa (Peterson and Butterfield, 2005).

The mitochondrial genome of Metazoa lacks introns, except in case of corals and sea anemones (Cnidaria), in which group I introns have been discovered in the *cox1* and *nad5* genes (Beagley et al., 1996; van Oppen et al., 2002). Infact, a recent cross-kingdom HGT of group I intron of *cox 1* gene in sponge *Tetilla* sp. (Spirophorida) mitochondrial genome from functional origin was reported, indicating the unexpected plasticity of the mitochondrial genomes of basal Metazoa (Rot et al., 2006).

A unique and first known case of HGT event of octocoral *mtMuTS* gene into animal mitochondrial genome has been reported (Bilewitch and Degnan, 2011), suggesting the need to reconsider the evolution of mitochondrial genome in metazoa.

## TRANSFER IN PHYLUM CNIDARIA

The phylum Cnidaria constitutes a diverse monophyletic group (Collins, 2002). Cnidarians have many different cell types, including gametes and nematocytes, which originate in the adult form from an interstitial cell lineage. Many of them are characterized by a complex, metagenetic life cycle including a sexually produced planula larva that metamorphoses into a sessile polyp stage, which may in turn asexually produce morphologically distinct, free-swimming, sexual medusae (Hyman, 1940).

Few instances of HGT were also seen in Cnidarians. For example, a subunit of bacterial poly-γ-glutamate (PGA) synthase was transferred to metazoan ancestor, suggesting its significant role on the evolution of stinging cells (nematocytes) in cnidarians (sea anemones, jellyfish, corals, etc.) (Denker et al., 2008). Hydra is simple freshwater animal which reproduce asexually by budding. The genome of *Hydra magnipapillata* contains 71 candidates for HGT, that show closer relationship to bacterial genes than metazoan genes and 70% of these are supported by ESTs (Chapman et al., 2010).

## PROTOSTOMIA

Protostomes are defined as a group of animals in which blastopore typically becomes the future mouth in most of the groups (Nielsen, 2001). It consists of two clades, Ecdysozoa and Spiralia (Spiralia or Lophotrochozoa). Ecdysozoa includes the following phyla: Nematoda, Nematomorpha, Tardigrada, Onychophora, Arthropoda, Priapulida, Loricifera, and Kinorhyncha (Figure 1) and Spiralia include two clades Platyzoa (Cavalier Smith, 1998) and Trochozoa (Roule, 1891).

Ecdysozoa are called as moulting protostomes and two phyla that are included under Ecdysozoa with reported cases of HGT include Nematoda and Arthropoda (Aguinaldo et al., 1997; Schmidt-Rhaesa et al., 1998; Edgecombe et al., 2000; Garey, 2001; Peterson and Eernisse, 2001; Zrzavý, 2003, Figure 1).

## TRANSFER IN PHYLUM NEMATODA

Nematodes represent the largest animal phylum, with an estimated number in the range of one to ten million species (Lambshead, 1993) and are found in virtually all habitats on earth. Many of them are parasites of plants and animals, including humans. The recent increase in nematode genomes has made attributions in comparative genomics to study the impact of HGT on their adaptation to new ecological niches. Although inter-kingdom HGT was initially controversial, it has been established with evidence of such recent events (Richards et al., 2011). Danchin et al. (2010) have studied the whole-genome sequences of root-knot nematodes and cyst-nematodes for the genes encoding proteins involved in the plant cell wall degradation and showed the incorporation of at least six distinct types of bacterial genes encoding proteins that can modify the plant cell wall into their genomes. These have subsequently undergone extensive gene duplication in the nematode lineages.

Furthermore, the cases of HGT from a diverse set of microorganisms into various nematode genomes have also been identified. The genome of *Bursaphelenchus xylophilus* (the pine wilt nematode) has incorporated six glycoside hydrolase family 16 (GH16) proteins from gammaproteobacteria, two hydrolases from *Firmicutes* and four aspartic-type endopeptidases and 11 GH45 cellulases from the Ascomycota of fungal origin (Kikuchi et al., 2011). The genome of *Pristionchus pacificus* (a necronemic nematode) suggests that it contains substantial amount of genes of insect origin (Rödelsperger and Sommer, 2011). The *Meloidogyne incognita* (the root-knot nematode) contains genes similar to those of actinobacteria, proteobacteria, and fungi (Abad et al., 2008). The plant-parasitic nematode *Heterodera glycines* contains a biosynthetic pathway for vitamin B6 of bacterial origin (Craig et al., 2008).

## TRANSFER IN PHYLUM ARTHROPODA

The members of the phylum Arthropoda are characterized by exoskeleton, segmented bodies and jointed appendages (Valentine, 2004). The appendages form part of an exoskeleton, which is mainly made of α-chitin, a derivative of glucose (Cutler, 1980). They are important members of marine, freshwater, land, and air ecosystems and are one of only two major animal groups that have adapted to life in dry environments (Ruppert et al., 2004). They include insects, arachnids, crustaceans, others and account for over 80% of all known living animal species (Anna, 2008).

The reported cases of HGT in insects include the acquisition of P elements by *Drosophila melanogaster* from *Drosophila willistoni* (Daniels et al., 1990; Engels, 1997), transfer of entire genes for carotenoid biosynthetic pathway from fungi (Moran and Jarvik, 2010) and 12 genes from bacteria (IAGC, 2010; Nikoh et al., 2010) to *Acyrthosiphon pisum*. The largest HGT transfer such as the transfer of entire *Wolbachia* bacterial genome (~1.4 Mb) into *Drosophila ananassae* Hawaii 2L chromosome (Hotopp et al., 2007) and also of *Wolbachia* to a wider range of insects such as pea aphid (Hemiptera), mosquitoes (Diptera), beetle (Coleoptera), fruit flies (Diptera), and parasitoid wasps (Hymenoptera) (Fenn et al., 2006; Hotopp et al., 2007) has been described. Recently, transfers have also been reported in *Bombyx*
*mori* that has acquired 10 genes from plant and bacteria (Zhu et al., 2011) and 14 types of 22 transferred genes from entomopathogenic bacteria, of which 13 types shared homology with sequences of Lepidopteran insects (Li et al., 2011), thus providing novel insights on the biological significance of HGT in the evolution of metazoan recipients.

## TRANSFER IN PHYLUM ROTIFERA

The Rotifera (also called Rotatoria) is a group of aquatic micrometazoans that usually occur in freshwater and marine environments (Wallace, 1998; Wallace et al., 2006; Segers, 2007) and are classified under Gnathifera of Platyzoa (Figure 1). The word “rotifer” is derived from a Latin word meaning “wheel-bearer” (Pechenik, 2005) and commonly called wheel animals due to the presence of corona in the cephalic region that resembles a wheel, which is used for locomotion and food gathering. They form the largest metazoan asexual group where no sexual reproduction has ever been reported and represent an ancient origin of asexuality with great evolutionary success in the diversification of the species (Mark Welch and Meselson, 2000; Fontaneto et al., 2007).

The first evidence of HGT has been reported in bdelloid rotifers to contain many foreign genes from diverse bacterial and eukaryotic origin, which are strongly concentrated in the telomeric regions along with diverse transposable elements (TEs) (Gladyshev et al., 2008, Figure 2). The evidence of extensive HGT in these asexual animals might be due to repeated cycles of desiccation-induced membrane disruption and DNA breakage and repair that occur as part of their life style.

The HGT in the asexual organisms (i.e., Rotifera and Hydra), suggests that they might have accumulated mutations in an irreversible manner in the absence of recombination through sexual reproduction (Muller, 1932, 1964; Felsenstein, 1974).

<figure><img src="image_4.png" /><figcaption>**FIGURE 2** | Horizontal gene transfer in *Bdelloid Rotifer*. *Bdelloid* rotifer contain diverse array of foreign genes acquired by HGT from various origin (like bacteria, metazoa, retrovirus, etc.). They are strongly concentrated in the telomeric region. The names of the transferred genes are indicated above the arrows.</figcaption></figure>

## DEUTEROSTOMIA

Deuterostomes are a group of animals in which the blastopore becomes the anus in the adult, while the mouth develops as a new opening from the end of the archenteron (Nielsen, 2001). It includes two main clades, Ambulacraria and Chordata. Phylum Chordata is again classified into Cephalochordata, Urochordata, and Craniata (incl. Vertebrata, see Figure 1).

## CRANIATA

The term “Craniata” was coupled with “Vertebrata” by Linnaeus (1758) to include lampreys, jawed fishes, and terrestrial vertebrates (tetrapods). The Craniata or craniates, are characterized by a skull (or cranium, hence their name). They comprise of all fishes including jawless fishes as hagfishes and lampreys, amphibians, reptiles, birds, and mammals, including Man. Now, the majority of the craniate species are represented by one group of fish, the actinopterygians, and the tetrapods (four-legged vertebrates) (Philippe, 1997).

## TRANSFER IN CLASS TELEOSTEI (FISHES)

Teleostei (the ray-finned fishes) represent one of the three classes of actinopterygii and includes most of the living fishes (Miller and Harley, 2007).

Cases of HGT in fishes are very sparse which include the transfer of lectin-like antifreeze proteins between them (Graham et al., 2008). Although the transfer of retroposons from *Schistosoma japonicum* (blood fluke) to salmonoid fishes was identified (Matveev and Okada, 2009), it was refuted with no evidence of such transfer between the two clades based on cross-species and vector contamination and was declared as erroneous report of HGT (Grunau and Boissier, 2010).

## TRANSFER IN CLASS MAMMALIA

With the burgeoning database of eukaryotic genomic sequences, it is not surprising to see the increasing cases of HGT in mammals. For example, transfer of DNA SPIN trasposons in mammals and other tetrapods (Pace et al., 2008) and transfer in human germ cells (Hecht et al., 2010) were reported. Recent studies have shown that the human body contains more of bacterial cells than human cells (Gill et al., 2006; Lester et al., 2006; Hehemann et al., 2010; Robinson et al., 2010), and many of them are dominated by members of *Bacteroidetes* and *Firmicutes* (Eckburg et al., 2005; Xu et al., 2007). Most of these recent transfers were driven by ecology rather than geography or phylogeny (Smillie et al., 2011).

In summary, the currently reported cases of HGT in animal kingdom are relatively low. However, with the availability of many new whole genome sequences, we can expect more incidences of HGT that will enable to gain further knowledge in the metazoan evolution.

## FATE OF TRANSFERRED GENES IN METAZOA

The identification of several HGT cases in animals has given rise to many questions such as the function of transferred genes, their evolutionary pathways and the forces governing the transferred genes. Indeed, efforts have been made recently to answer these questions. For example, in nematodes, recent work using 454-sequencing on cellulase functioning genes has shown that they
have integrated into receptor genome by providing special func-
tions, which indicates that genes continue to evolve with several
gene duplications or deletions and DNA substitution rates after
the HGT event (Mayer et al., 2011). Most of them encode enzymes
for the cell wall degradation in plants and fungi and play vital
role in the biology of the nematodes (Abad et al., 2008; Kikuchi
et al., 2011; Mayer et al., 2011). The most recent progress has been
made about the functional role of cell wall degrading enzymes of
plant-parasitic nematode (Haegeman et al., 2012).

The acquisition of two enzymes for vitamin B6 biosynthe-
sis in plant pathogenic nematode *Heterodera glycines* suggests
host-parasite interactions (Craig et al., 2008). Incorporation of
bacterial genes by specialized animal rumen parasites, i.e., *Giardia
lamblia* (Morrison et al., 2007), *Trichomonas vaginalis* (Carlton
et al., 2007) and *Entamoeba histolytica* (Loftus et al., 2005), that
exist in anaerobic environments suggests that adaptation to par-
asitism might also favor the acquisition of new genes by HGT.
Transfer of lectin-like antifreeze proteins in arctic fish might
have favored them to survive in cold-conditions (Graham et al.,
2008). The presence of PGA synthase genes in cnidarians might
have contributed to the evolution of nematocytes, that help in
prey capture (Denker et al., 2008). Recent studies on transfer in
bdelloid rotifers, which has acquired genes from bacterial and
eukaryotic origin, some of them are expressed suggests that they
may possibly provide novel metabolic functions to these asex-
ual animals (Gladyshev et al., 2008). The transfer of bacteria to
human (Robinson et al., 2010) is interesting because it may be
important to human health, have the potential to provide novel
functions, there by affecting the evolution.

In summary, these results support that the transferred genes
having a functional role are retained, while useless genes are elimi-
nated. The studies outlined on the function of transferred genes in
animals are still rudimentary. Hence, there is need to understand
the function of transferred genes in new genomes by involving
robust phylogenetic investigations and biological disciplines.

Besides HGT, other genetic events such as transfer by retro-
transposons and hybridization (Seehausen, 2004) were also
reported in metazoa, which may form additional limitations in
the reconstruction of animal TOL.

RETROTRANSPOSONS AND THEIR CLASSIFICATION

Retrotransposons (retroelements) belong to group of TEs. TEs
(also known as “jumping genes”) include a diverse array of DNA
sequences and possess the inherent capacity to self-reproduce and
move within and between genomes. Ever since their discovery in
maize DNA (McClintock, 1956), TEs have been found in genomes
of almost all organisms. They constitute more than 50% of maize
(Zea mays) genome (Kidwell and Lisch, 1997; Wessler, 1998), 22%
of *Drosophila* genome (Kapitonov and Jurka, 2003) and half of
our human genome with just 1.5% coding for protein region
(Lander et al., 2001).

TEs are divided into two groups based on their transpo-
sition mechanism and sequence organization (Finnegan, 1989;
Capy, 1998): 1. DNA transposons (move predominantly via a
DNA-mediated mechanism of excision and insertion and con-
stitute appx. 3% of human genome (Craig et al., 2002) and
2. Retroelements (move by reverse transcription of an RNA

intermediate (Rogers, 1985) and include the retrotransposons, eukaryotic TEs, group II mitochondrial introns, bacterial retroelements is usually encoded by the element itself. They are subdivided into two major groups based on the presence or the absence of long terminal repeats, which flank the body of the element: long terminal repeat (LTR)-containing elements (LTRs) and non-LTR retrotransposons (non-LTR). Again the non-LTRs are subdivided into two classes: LINEs or L1-element (long interspersed elements) and SINEs (short interspersed elements) (Weiner et al., 1986). LINEs, the autonomous elements are widely distributed in eukaryotes. For example, they occur in >500, 000 copies (~17%) in human genome (Lander et al., 2001), out of which only ~80–100 were found to be active (Brouha et al., 2003). They are also found in mouse genome (~3000, Goodier et al., 2001) and *Drosophila* genome (Priimagi et al., 1988; Levis et al., 1993; Udomkit et al., 1995). Unlike LINEs, SINEs are non-autonomous and occupy about 12% of the human genome, out of which majority of them belong to Alu elements (Lander et al., 2001). The classification of TEs is shown in **Figure 3**.

TRANSFER BY RETROTRANSPOSONS

With the widespread distribution of intergenomic TEs in eukary-
otic genomes, it is not surprising to envisage the intriguing feature
of HGT among them, a process by which they cross-species
boundaries to enter new genomes. In the past decade, substantial
evidence of HGT has been reported for all types of TEs in inver-
tebrates as well as vertebrates. Some of the reported cases of HGT
involving TEs are summarized in **Table 1** as shown below.

In addition to these, the vertebrate genomes also contain
numerous copies of retroviral sequences that were acquired over
the course of evolution. The majority of them belong to endoge-
nous viral elements, which integrate into the nuclear genome of
the host germ line (Tristem, 2000; Lander et al., 2001). As it is
exhaustive to provide all the reported cases of retroviral elements,
we present few of them. They include the human endogenous
retrovirus element HERV-L, that is related distantly by homol-
ogy to foamy viruses (Cordonnier et al., 1995), and recently
reported cases such as the presence of endogenous viral elements
in animal genomes (Katzourakis and Gifford, 2010), integration
of ancient bornavirus and ebolavirus/marburgvirus sequences in

<figure><img src="image_5.png" /><figcaption>**FIGURE 3** | Classification of transposable elements. (A) Long terminal repeats, (B) Non-long terminal repeats, (C) Long interspersed elements, (D) Short interspersed elements.</figcaption></figure>
<table><caption>Table 1 | Table showing some of the cases of horizontal gene transfer involving Transposable Elements (TEs) among Metazoa.</caption><thead><tr><th colspan="3">Transposable elements</th></tr><tr><th>Type</th><th>Metazoan group/genome</th><th>Reference</th></tr></thead><tbody><tr><td>LINE/jockey</td><td>Arthropoda/Drosophila</td><td>Mizrokhi and Mazo, 1990</td></tr><tr><td>LINE/Bov-B</td><td>Mammals</td><td>Kordis and Gubensek, 1995</td></tr><tr><td>SINE/Smal-cor</td><td>Teleostei (coregonid fish)</td><td>Hamada et al., 1997</td></tr><tr><td>LTR/copia</td><td>Arthropoda/Drosophila</td><td>Jordan et al., 1999</td></tr><tr><td>SURL elements</td><td>Echinodermata</td><td>Gonzalez and Lessios, 1999.</td></tr><tr><td>LTR/gypsy</td><td>Arthropoda/Drosophila</td><td>Vazquez-Manrique et al., 2000</td></tr><tr><td>LTR/gypsy</td><td>Arthropoda/Drosophila</td><td>Terzian et al., 2000</td></tr><tr><td>LINE/Rex1</td><td>Teleostei (fish genomes)</td><td>Volff et al., 2000</td></tr><tr><td>LINE/Bov-B</td><td>Reptiles and Mammals</td><td>Zupunski et al., 2001</td></tr><tr><td>P elements</td><td>Arthropoda/Drosophila</td><td>Daniels et al., 1990</td></tr><tr><td>P elements</td><td>Arthropoda/Drosophila</td><td>Hagemann et al., 1992</td></tr><tr><td>mariner elements</td><td>Arthropoda/ Drosophila mauritiana and Zaprionus tuberculatus</td><td>Maruyama and Hartl, 1991</td></tr><tr><td>mariner elements</td><td>Arthropoda</td><td>Robertson and MacLeod, 1993</td></tr><tr><td>mariner elements</td><td>Arthropoda/Drosophila</td><td>Brunet et al., 1994</td></tr><tr><td>mariner elements</td><td>Arthropoda/Drosophila</td><td>Lohe et al., 1995</td></tr><tr><td>mariner elements</td><td>Arthropoda</td><td>Robertson and Lampe, 1995</td></tr><tr><td>mariner elements</td><td>Mammals/Homo sapiens</td><td>Smit and Riggs, 1996</td></tr><tr><td>mariner elements</td><td>Amphibians</td><td>Lam et al., 1996</td></tr><tr><td>mariner elements</td><td>Platyzoa and Cnidaria</td><td>Robertson, 1997</td></tr><tr><td>mariner elements</td><td>Mammals</td><td>Robertson et al., 2002</td></tr><tr><td>DNA SPIN transposons</td><td>Mammals and other tetrapods</td><td>Pace et al., 2008</td></tr><tr><td>Helitrons (rolling circle DNA transposons)</td><td>Arthropoda, Reptiles, Teleostei (fish) and Mammals</td><td>Thomas et al., 2010</td></tr></tbody></table>

vertebrate genomes (Belyi et al., 2010), endogenous lentivirus in basal primates (Gifford et al., 2008; Gilbert et al., 2009) and endogenous foamy viruses in the sloth genome (Katzourakis et al., 2009).

All these results suggest that retrotransposons continue to play an active role in shaping the dynamics of metazoan evolution by forming new genes and thus contributing to the chimeric evolution of genomes. The representation of metazoan chimeric evolution of genomes is shown in **Figure 4**.

## HYBRIDIZATION

Hybridization, the exchange of genes between closely related species by sexual reproduction is a natural evolutionary process. The frequency of hybridization among species is common although it is rare between species on a per-individual basis. About 10–30% of multicellular animal and plant species hybridize regularly (Mallet, 2005). Closely related species tend to hybridize more. Therefore, species-rich groups in rapidly diversifying adaptive radiations tend to be those that hybridize most (Price and Bouvier, 2002; Seehausen, 2004; Gourbière and Mallet, 2010). However, the success of large number of hybridizations that occur among closely related species depends on genetic and environmental factors (Arnold et al., 2012).

Although recent studies have highlighted the events of HGT, information about hybridization is limited and highly unexplored, especially in the case of animals. However, studies have reported the cases of hybridization between more closely related lineages such as fungi, plants, and even vertebrate lineages such as

amphibians, fish, and birds (Arnold, 2006). Recently, studies on primates have detected hybridization in not only between species and subspecies but also between genera, including human lineage (Zinner et al., 2011).

Hybridization among species can thus act as a catalyst for the formation of new lineages.

<figure><img src="image_6.png" /><figcaption>**FIGURE 4 | Metazoan chimeric evolution.** The figure represents chimeric nature of metazoan genomes with horizontal gene transfer, retrotransposons, and hybridization events. Horizontal gene transfer from (A) Bacteria, (B) Retrotransposons, (C) Fungi, (D) Plants and (E) Hybridization between closely related species (indicated by blue arrows).</figcaption></figure>
## CONCLUDING REMARKS

The relative amount of HGT events detected in metazoa merely represent just tip of an iceberg. Currently, the reported rate of HGT in animal kingdom is relatively low and still in the state of infancy. There are still many outstanding questions that need to be addressed like: What is the rate of gene transfer within the group? How important is gene transfer in other animal genomes, especially vertebrates? There are many lineages that need to be considered for HGT, such as Rotifers, Aves (birds), Reptiles and Mammals. Why are eukaryotic genes rare in prokaryotic genomes? What is the function of transferred genes in the new genome?

Although, there is substantial evidence on transfer of TEs (especially group I introns) in the mitochondrial genomes of metazoa (ex.sponges), we are unaware on the extent of these transfers in other groups (especially fishes, rotifers, and other vertebrates), which might serve as useful indicators, raising intriguing biological questions related to HGT. The recent evidence of octocoral *mtMuTS* gene into animal mitochondrial genome also support these findings,

thus challenging the evolution of mitochondrial genome in metazoa.

Therefore, there is an emergent need for future HGT studies in Metazoa. Hopefully, with the recent improvements in new sequencing technologies and increased number of diverse vertebrate genomes, we anticipate to gain novel insights into the role played by HGT that will shed light in understanding the metazoan evolution.

Given the extent of gene transfer from different origins (bacteria, plants, fungi, and eukaryotes), transfer by retrotransposons and the fusion of lineages to from new lines of descent (inter species hybridization) in animals, we believe that majority of the animal genomes exhibit mosaic structure and chimerism, thus replacing the concept of animal TOL with a new paradigm. Therefore, the question still remains unanswered about the evolution of metazoa, as whether it can be represented in the form of a network or rhizome and needs to be determined.

## ACKNOWLEDGMENTS

We would like to thank Infectiopole Sud for funding.

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**Conflict of Interest Statement:** The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Received: 13 February 2012; accepted: 23 March 2012; published online: 11 April 2012.

Citation: Ramulu HG, Raoult D and Pontarotti P (2012) *The rhizome of life: what about metazoa?*. *Front. Cell. Inf. Microbio.* 2:50. doi: 10.3389/fcimb.2012.00050

Copyright © 2012 Ramulu, Raoult and Pontarotti. This is an open-access article distributed under the terms of the Creative Commons Attribution Non Commercial License, which permits non-commercial use, distribution, and reproduction in other forums, provided the original authors and source are credited.