# Seven years of microbial community metagenomes from temperate soils affected by an ongoing coal seam fire

Samuel E Barnett, Ashley Shade

► To cite this version:

Samuel E Barnett, Ashley Shade. Seven years of microbial community metagenomes from temper-
ate soils affected by an ongoing coal seam fire. Microbiology Resource Announcements, In press,
10.1128/mra.00198-24. hal-04590347

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1 Seven years of microbial community metagenomes from temperate soils affected by an ongoing

2 coal seam fire

3

4 Samuel E. Barnett¹, Ashley Shade²#

5

6 ¹Department of Microbiology, Genetics, and Immunology, Michigan State University, East

7 Lansing, MI 48824, USA

8 ²Université Claude Bernard Lyon 1; CNRS, UMR 5557 Écologie Microbienne, 16 rue Dubois

9 69622 Villeurbanne Cedex, France

10

11 Running title: Seven-year metagenome timeseries from Centralia, PA soils

12

13 #Address correspondence to Ashley Shade: ashley.shade@cnrs.fr

14
1 Seven years of microbial community metagenomes from temperate soils affected by an ongoing

2 coal seam fire

3

4 Samuel E. Barnett¹, Ashley Shade²#

5

6 ¹Department of Microbiology, Genetics, and Immunology, Michigan State University, East

7 Lansing, MI 48824, USA

8 ²Université Claude Bernard Lyon 1; CNRS, UMR 5557 Écologie Microbienne, 16 rue Dubois

9 69622 Villeurbanne Cedex, France

10

11 Running title: Seven-year metagenome timeseries from Centralia, PA soils

12

13 #Address correspondence to Ashley Shade: ashley.shade@cnrs.fr

14
15 Abstract

We examined the dynamics of soil microbiomes under heat press disturbance from an
underground coal mine fire in Centralia, Pennsylvania. Here we present metagenomic
sequencing and assembly data from soil microbiomes across seven consecutive years at
repeatedly sampled fire affected sites along with unaffected reference sites.

20

21 Announcement

Anthropogenic pressures on the environment fundamentally change ecosystems and their
functions, in part by altering microbial communities responsible for important ecosystem
services. As many anthropogenic disturbances act long-term such "press" disturbances require
committed, interannual observations to observe and understand the resilience of microbial
communities and their functional consequences. The six-decade-old coal mine fire in Centralia,
Pennsylvania, USA, provides a unique case study to examine the resilience of soil microbial
communities to an intense, long-term thermal disturbance (1). We expanded upon previous
research at Centralia (2-4) by sequencing metagenomes annually collected from ten sites over
seven consecutive years.

Sampling sites in Centralia Pennsylvania USA have been previously discussed (4). For
metagenome sequencing, we utilized three reference sites (i.e., unheated soils) and seven fire
affected sites (i.e., actively heated soils). 20 cm deep soil cores were collected using a sterilized
PVC pipe corer or trowel the first or second week in October yearly between 2015 and 2021,
immediately sieved to 4 mm and flash frozen in liquid N₂ in the field and then stored in the lab at
-80°C as previously described (4). DNA was extracted from 0.25 g of each soil using phenol-
chloroform extraction (5) with BeadBug homogenizer tubes (#Z763764; Benchmark Scientific,
15 Abstract

We examined the dynamics of soil microbiomes under heat press disturbance from an
underground coal mine fire in Centralia, Pennsylvania. Here we present metagenomic
sequencing and assembly data from soil microbiomes across seven consecutive years at
repeatedly sampled fire affected sites along with unaffected reference sites.

20

21 Announcement

Anthropogenic pressures on the environment fundamentally change ecosystems and their
functions, in part by altering microbial communities responsible for important ecosystem
services. As many anthropogenic disturbances act long-term such "press" disturbances require
committed, interannual observations to observe and understand the resilience of microbial
communities and their functional consequences. The six-decade-old coal mine fire in Centralia,
Pennsylvania, USA, provides a unique case study to examine the resilience of soil microbial
communities to an intense, long-term thermal disturbance (1). We expanded upon previous
research at Centralia (2-4) by sequencing metagenomes annually collected from ten sites over
seven consecutive years.

Sampling sites in Centralia Pennsylvania USA have been previously discussed (4). For
metagenome sequencing, we utilized three reference sites (i.e., unheated soils) and seven fire
affected sites (i.e., actively heated soils). 20 cm deep soil cores were collected using a sterilized
PVC pipe corer or trowel the first or second week in October yearly between 2015 and 2021,
immediately sieved to 4 mm and flash frozen in liquid N₂ in the field and then stored in the lab at
-80°C as previously described (4). DNA was extracted from 0.25 g of each soil using phenol-
chloroform extraction (5) with BeadBug homogenizer tubes (#Z763764; Benchmark Scientific,
38 Sayreville USA). Bead beating was performed for 60 s on a Mini-Beadbeater 16 (BioSpec
39 Products Inc., Bartlesville, USA). Illumina shotgun metagenomic DNA libraries were prepared
40 and sequenced by the Research Technology Support Facility at Michigan State University (East
41 Lansing, MI, USA). Libraries were generated, barcoded with unique dual indexes, and pooled
42 using the ThruPLEX DNA-Seq Kit (Takara Bio Inc., Kusatsu, Japan) following standard
43 protocols. At this stage, one sample was removed due to failed library preparation and the
44 remaining 69 other samples were pooled. Metagenomic sequencing was performed in two lanes
45 of a NovaSeq S4 flow cell in 2x150bp paired-end format on a NovaSeq 6000 (Illumina, San
46 Diego, USA).

47 Metagenomic sequencing reads were processed using a custom workflow based on the
48 Joint Genome Institute SOP (6). Raw reads were first quality trimmed and filtered using bbduk
49 from BBMap version 38.18 (7). Filtered reads were then error corrected using bbcms from
50 BBMap version 38.18 (7). Quality controlled reads were then assembled within individual
51 samples using SPAdes version 3.15.5 in metaSPAdes mode (8) with assembler only. Contigs
52 under 1000 bp were removed and remaining contig taxonomy was determined using Kraken
53 version 2.1.3 (9).

54 Combined runs generated between 47 million and 172 million quality filtered reads
55 (Table 1). Assemblies produced between 18,000 and 192,000 contigs longer than 1000 bp with
56 N50 values ranging from 1458-11958 (Table 1). Between 58.8 and 79.9% of contigs were
57 classified by Kraken to the phylum level. Of these contigs, the top 10 phyla represented were
58 Pseudomonadota (50.4% on average), Actinomycetota (37.1%), Planctomycetota (3.1%),
59 Acidobacteriota (2.6%), Myxococcota (1.4%), Bacillota (1.2%), Bacteroidota (0.9%),
60 Chloroflexota (0.5%), Thermodesulfobacteriota (0.4%), and Cyanobacteriota (0.4%) (Fig. 1).
38 Sayreville USA). Bead beating was performed for 60 s on a Mini-Beadbeater 16 (BioSpec
39 Products Inc., Bartlesville, USA). Illumina shotgun metagenomic DNA libraries were prepared
40 and sequenced by the Research Technology Support Facility at Michigan State University (East
41 Lansing, MI, USA). Libraries were generated, barcoded with unique dual indexes, and pooled
42 using the ThruPLEX DNA-Seq Kit (Takara Bio Inc., Kusatsu, Japan) following standard
43 protocols. At this stage, one sample was removed due to failed library preparation and the
44 remaining 69 other samples were pooled. Metagenomic sequencing was performed in two lanes
45 of a NovaSeq S4 flow cell in 2x150bp paired-end format on a NovaSeq 6000 (Illumina, San
46 Diego, USA).

47 Metagenomic sequencing reads were processed using a custom workflow based on the
48 Joint Genome Institute SOP (6). Raw reads were first quality trimmed and filtered using bbduk
49 from BBMap version 38.18 (7). Filtered reads were then error corrected using bbcms from
50 BBMap version 38.18 (7). Quality controlled reads were then assembled within individual
51 samples using SPAdes version 3.15.5 in metaSPAdes mode (8) with assembler only. Contigs
52 under 1000 bp were removed and remaining contig taxonomy was determined using Kraken
53 version 2.1.3 (9).

54 Combined runs generated between 47 million and 172 million quality filtered reads
55 (Table 1). Assemblies produced between 18,000 and 192,000 contigs longer than 1000 bp with
56 N50 values ranging from 1458-11958 (Table 1). Between 58.8 and 79.9% of contigs were
57 classified by Kraken to the phylum level. Of these contigs, the top 10 phyla represented were
58 Pseudomonadota (50.4% on average), Actinomycetota (37.1%), Planctomycetota (3.1%),
59 Acidobacteriota (2.6%), Myxococcota (1.4%), Bacillota (1.2%), Bacteroidota (0.9%),
60 Chloroflexota (0.5%), Thermodesulfobacteriota (0.4%), and Cyanobacteriota (0.4%) (Fig. 1).
61

62 **Data availability**

63 Raw reads and assembled contigs at least 1000 bp long are available through NCBI in the SRA

64 and genome databases respectively, both under BioProject PRJNA974462. Raw read and
65 assembly accessions are indicated in Table 1. Code for read processing and assembly are
66 available on GitHub at

67 https://github.com/ShadeLab/Centralia_7year_metagenome_processing_Barnett_2024.

68

69 **Acknowledgements**

70 This work was supported by the U.S. National Science Foundation CAREER award #1749544 to
71 AS.

72

73 **References**

74 1. Shade A. 2018. Understanding Microbiome Stability in a Changing World. mSystems
75 3:e00157-17.

76 2. Lee S-H, Sorensen JW, Grady KL, Tobin TC, Shade A. 2017. Divergent extremes but
77 convergent recovery of bacterial and archaeal soil communities to an ongoing
78 subterranean coal mine fire. ISME J 11:1447-1459.

79 3. Kearns PJ, Shade A. 2018. Trait-based patterns of microbial dynamics in dormancy
80 potential and heterotrophic strategy: case studies of resource-based and post-press
81 succession. ISME J 12:2575-2581.

82 4. Barnett SE, Shade A. 2024. Arrive and wait: Inactive bacterial taxa contribute to
83 perceived soil microbiome resilience after a multidecadal press disturbance. Ecol Lett
61

62 **Data availability**

63 Raw reads and assembled contigs at least 1000 bp long are available through NCBI in the SRA

64 and genome databases respectively, both under BioProject PRJNA974462. Raw read and
65 assembly accessions are indicated in Table 1. Code for read processing and assembly are
66 available on GitHub at

67 https://github.com/ShadeLab/Centralia_7year_metagenome_processing_Barnett_2024.

68

69 **Acknowledgements**

70 This work was supported by the U.S. National Science Foundation CAREER award #1749544 to
71 AS.

72

73 **References**

74 1. Shade A. 2018. Understanding Microbiome Stability in a Changing World. mSystems
75 3:e00157-17.

76 2. Lee S-H, Sorensen JW, Grady KL, Tobin TC, Shade A. 2017. Divergent extremes but
77 convergent recovery of bacterial and archaeal soil communities to an ongoing
78 subterranean coal mine fire. ISME J 11:1447-1459.

79 3. Kearns PJ, Shade A. 2018. Trait-based patterns of microbial dynamics in dormancy
80 potential and heterotrophic strategy: case studies of resource-based and post-press
81 succession. ISME J 12:2575-2581.

82 4. Barnett SE, Shade A. 2024. Arrive and wait: Inactive bacterial taxa contribute to
83 perceived soil microbiome resilience after a multidecadal press disturbance. Ecol Lett
84 27:e14393.

85 5. Griffiths RI, Whiteley AS, O'Donnell AG, Bailey MJ. 2000. Rapid Method for

86 Coextraction of DNA and RNA from Natural Environments for Analysis of Ribosomal

87 DNA- and rRNA-Based Microbial Community Composition. Appl Environ Microbiol
88 66:5488-5491.

89 6. Clum A, Huntemann M, Bushnell B, Foster B, Foster B, Roux S, Hajek PP, Varghese N,
90 Mukherjee S, Reddy TBK, Daum C, Yoshinaga Y, O'Malley R, Seshadri R, Kyrpides NC,
91 Eloe-Fadrosh EA, Chen I-MA, Copeland A, Ivanova NN. 2021. DOE JGI Metagenome
92 Workflow. mSystems 6:e00804-20.

93 7. Bushnell B. 2019. BBMap short-read aligner, and other bioinformatics tools.

94 8. Nurk S, Meleshko D, Korobeynikov A, Pevzner PA. 2017. metaSPAdes: a new versatile
95 metagenomic assembler. Genome Res 27:824-834.

96 9. Wood DE, Lu J, Langmead B. 2019. Improved metagenomic analysis with Kraken 2.
97 Genome Biol 20:257.

98
84 27:e14393.

85 5. Griffiths RI, Whiteley AS, O'Donnell AG, Bailey MJ. 2000. Rapid Method for

86 Coextraction of DNA and RNA from Natural Environments for Analysis of Ribosomal

87 DNA- and rRNA-Based Microbial Community Composition. Appl Environ Microbiol
88 66:5488-5491.

89 6. Clum A, Huntemann M, Bushnell B, Foster B, Foster B, Roux S, Hajek PP, Varghese N,
90 Mukherjee S, Reddy TBK, Daum C, Yoshinaga Y, O'Malley R, Seshadri R, Kyrpides NC,
91 Eloe-Fadrosh EA, Chen I-MA, Copeland A, Ivanova NN. 2021. DOE JGI Metagenome
92 Workflow. mSystems 6:e00804-20.

93 7. Bushnell B. 2019. BBMap short-read aligner, and other bioinformatics tools.

94 8. Nurk S, Meleshko D, Korobeynikov A, Pevzner PA. 2017. metaSPAdes: a new versatile
95 metagenomic assembler. Genome Res 27:824-834.

96 9. Wood DE, Lu J, Langmead B. 2019. Improved metagenomic analysis with Kraken 2.
97 Genome Biol 20:257.

98
<table><thead><tr><td>Sample ID</td><td>Site</td><td>Collection date</td><td>Soil Temp. (°C)</td><td>Fire classification</td><td>Raw read accession</td><td>No. filtered reads</td><td>Assembly accession</td><td>No. contigs</td><td>Assembly length (bp)</td><td>N50 (bp)</td><td>Longest Contig (bp)</td></tr></thead><tbody><tr><td>Cen08_13102015</td><td>Cen08</td><td>13-Oct-2015</td><td>12.7</td><td>Reference</td><td>SRR24677618</td><td>124327406</td><td>JBAIFX000000000</td><td>160124</td><td>351137115</td><td>2279</td><td>658717</td></tr><tr><td>Cen08_12102016</td><td>Cen08</td><td>12-Oct-2016</td><td>11.1</td><td>Reference</td><td>SRR24677617</td><td>86715826</td><td>JBAIFY000000000</td><td>101237</td><td>243866855</td><td>2683</td><td>1053386</td></tr><tr><td>Cen08_21102017</td><td>Cen08</td><td>21-Oct-2017</td><td>13.3</td><td>Reference</td><td>SRR24677606</td><td>75877554</td><td>JBAIFZ000000000</td><td>74881</td><td>186130345</td><td>2816</td><td>155567</td></tr><tr><td>Cen08_04102018</td><td>Cen08</td><td>4-Oct-2018</td><td>16.1</td><td>Reference</td><td>SRR24677595</td><td>87148206</td><td>JBAIGA000000000</td><td>69059</td><td>177362065</td><td>3067</td><td>303328</td></tr><tr><td>Cen08_15102020</td><td>Cen08</td><td>15-Oct-2020</td><td>11.6</td><td>Reference</td><td>SRR24677584</td><td>80638542</td><td>JBAIGB000000000</td><td>72853</td><td>195537628</td><td>3389</td><td>325882</td></tr><tr><td>Cen08_06102021</td><td>Cen08</td><td>6-Oct-2021</td><td>15.8</td><td>Reference</td><td>SRR24677573</td><td>105866020</td><td>JBAIGC000000000</td><td>82965</td><td>234730043</td><td>3720</td><td>631307</td></tr><tr><td>Cen11_12102015</td><td>Cen11</td><td>12-Oct-2015</td><td>27.4</td><td>FireAffected</td><td>SRR24677562</td><td>111788990</td><td>JBAIGD000000000</td><td>138367</td><td>288073727</td><td>2024</td><td>278524</td></tr><tr><td>Cen11_11102016</td><td>Cen11</td><td>11-Oct-2016</td><td>25.1</td><td>FireAffected</td><td>SRR24677552</td><td>98625612</td><td>JBAIGE000000000</td><td>129881</td><td>281691484</td><td>2221</td><td>647046</td></tr><tr><td>Cen11_20102017</td><td>Cen11</td><td>20-Oct-2017</td><td>23.2</td><td>FireAffected</td><td>SRR24677551</td><td>114203656</td><td>JBAIGF000000000</td><td>163879</td><td>374766051</td><td>2358</td><td>895580</td></tr><tr><td>Cen11_03102018</td><td>Cen11</td><td>3-Oct-2018</td><td>21.8</td><td>FireAffected</td><td>SRR24677550</td><td>119434650</td><td>JBAIGG000000000</td><td>144335</td><td>345423017</td><td>2626</td><td>987027</td></tr><tr><td>Cen11_06102019</td><td>Cen11</td><td>6-Oct-2019</td><td>19.3</td><td>FireAffected</td><td>SRR24677616</td><td>68277302</td><td>JBAIGH000000000</td><td>82766</td><td>201039944</td><td>2701</td><td>855103</td></tr><tr><td>Cen11_13102020</td><td>Cen11</td><td>13-Oct-2020</td><td>19.5</td><td>FireAffected</td><td>SRR24677615</td><td>108943344</td><td>JBAIGI000000000</td><td>118956</td><td>238107306</td><td>2047</td><td>71568</td></tr><tr><td>Cen11_06102021</td><td>Cen11</td><td>6-Oct-2021</td><td>20</td><td>FireAffected</td><td>SRR24677614</td><td>93275172</td><td>JBAIGJ000000000</td><td>71500</td><td>151498571</td><td>2182</td><td>303453</td></tr><tr><td>Cen14_12102015</td><td>Cen14</td><td>12-Oct-2015</td><td>28.9</td><td>FireAffected</td><td>SRR24677613</td><td>123668420</td><td>JBAIGK000000000</td><td>117428</td><td>374847644</td><td>4394</td><td>901286</td></tr><tr><td>Cen14_11102016</td><td>Cen14</td><td>11-Oct-2016</td><td>29.4</td><td>FireAffected</td><td>SRR24677612</td><td>126966694</td><td>JBAIGL000000000</td><td>110577</td><td>403592710</td><td>6604</td><td>937175</td></tr><tr><td>Cen14_19102017</td><td>Cen14</td><td>19-Oct-2017</td><td>28.5</td><td>FireAffected</td><td>SRR24677611</td><td>122282866</td><td>JBAIGM000000000</td><td>121111</td><td>318405198</td><td>3155</td><td>717853</td></tr><tr><td>Cen14_02102018</td><td>Cen14</td><td>2-Oct-2018</td><td>30</td><td>FireAffected</td><td>SRR24677610</td><td>105414742</td><td>JBAIGN000000000</td><td>118460</td><td>387227442</td><td>4877</td><td>901280</td></tr><tr><td>Cen14_06102019</td><td>Cen14</td><td>6-Oct-2019</td><td>26.9</td><td>FireAffected</td><td>SRR24677609</td><td>101355352</td><td>JBAIGO000000000</td><td>123103</td><td>358796064</td><td>3771</td><td>883014</td></tr><tr><td>Cen14_13102020</td><td>Cen14</td><td>13-Oct-2020</td><td>25.4</td><td>FireAffected</td><td>SRR24677608</td><td>92846146</td><td>JBAIGP000000000</td><td>106684</td><td>262291366</td><td>2670</td><td>1293213</td></tr><tr><td>Cen14_05102021</td><td>Cen14</td><td>5-Oct-2021</td><td>22.1</td><td>FireAffected</td><td>SRR24677607</td><td>134497346</td><td>JBAIGQ000000000</td><td>97150</td><td>239260764</td><td>2798</td><td>1265966</td></tr><tr><td>Cen15_12102015</td><td>Cen15</td><td>12-Oct-2015</td><td>35.3</td><td>FireAffected</td><td>SRR24677605</td><td>125281422</td><td>JBAIGR000000000</td><td>128596</td><td>440403073</td><td>5157</td><td>1037399</td></tr><tr><td>Cen15_11102016</td><td>Cen15</td><td>11-Oct-2016</td><td>31.8</td><td>FireAffected</td><td>SRR24677604</td><td>153739606</td><td>JBAIGS000000000</td><td>120958</td><td>459008620</td><td>7193</td><td>960662</td></tr><tr><td>Cen15_19102017</td><td>Cen15</td><td>19-Oct-2017</td><td>27.2</td><td>FireAffected</td><td>SRR24677603</td><td>123055626</td><td>JBAIGT000000000</td><td>145031</td><td>425783382</td><td>3797</td><td>813692</td></tr><tr><td>Cen15_02102018</td><td>Cen15</td><td>2-Oct-2018</td><td>28.6</td><td>FireAffected</td><td>SRR24677602</td><td>137128144</td><td>JBAIGU000000000</td><td>144158</td><td>397881104</td><td>3459</td><td>643680</td></tr></tbody></table>
<table><thead><tr><td>Sample ID</td><td>Site</td><td>Collection date</td><td>Soil Temp. (°C)</td><td>Fire classification</td><td>Raw read accession</td><td>No. filtered reads</td><td>Assembly accession</td><td>No. contigs</td><td>Assembly length (bp)</td><td>N50 (bp)</td><td>Longest Contig (bp)</td></tr></thead><tbody><tr><td>Cen08_13102015</td><td>Cen08</td><td>13-Oct-2015</td><td>12.7</td><td>Reference</td><td>SRR24677618</td><td>124327406</td><td>JBAIFX000000000</td><td>160124</td><td>351137115</td><td>2279</td><td>658717</td></tr><tr><td>Cen08_12102016</td><td>Cen08</td><td>12-Oct-2016</td><td>11.1</td><td>Reference</td><td>SRR24677617</td><td>86715826</td><td>JBAIFY000000000</td><td>101237</td><td>243866855</td><td>2683</td><td>1053386</td></tr><tr><td>Cen08_21102017</td><td>Cen08</td><td>21-Oct-2017</td><td>13.3</td><td>Reference</td><td>SRR24677606</td><td>75877554</td><td>JBAIFZ000000000</td><td>74881</td><td>186130345</td><td>2816</td><td>155567</td></tr><tr><td>Cen08_04102018</td><td>Cen08</td><td>4-Oct-2018</td><td>16.1</td><td>Reference</td><td>SRR24677595</td><td>87148206</td><td>JBAIGA000000000</td><td>69059</td><td>177362065</td><td>3067</td><td>303328</td></tr><tr><td>Cen08_15102020</td><td>Cen08</td><td>15-Oct-2020</td><td>11.6</td><td>Reference</td><td>SRR24677584</td><td>80638542</td><td>JBAIGB000000000</td><td>72853</td><td>195537628</td><td>3389</td><td>325882</td></tr><tr><td>Cen08_06102021</td><td>Cen08</td><td>6-Oct-2021</td><td>15.8</td><td>Reference</td><td>SRR24677573</td><td>105866020</td><td>JBAIGC000000000</td><td>82965</td><td>234730043</td><td>3720</td><td>631307</td></tr><tr><td>Cen11_12102015</td><td>Cen11</td><td>12-Oct-2015</td><td>27.4</td><td>FireAffected</td><td>SRR24677562</td><td>111788990</td><td>JBAIGD000000000</td><td>138367</td><td>288073727</td><td>2024</td><td>278524</td></tr><tr><td>Cen11_11102016</td><td>Cen11</td><td>11-Oct-2016</td><td>25.1</td><td>FireAffected</td><td>SRR24677552</td><td>98625612</td><td>JBAIGE000000000</td><td>129881</td><td>281691484</td><td>2221</td><td>647046</td></tr><tr><td>Cen11_20102017</td><td>Cen11</td><td>20-Oct-2017</td><td>23.2</td><td>FireAffected</td><td>SRR24677551</td><td>114203656</td><td>JBAIGF000000000</td><td>163879</td><td>374766051</td><td>2358</td><td>895580</td></tr><tr><td>Cen11_03102018</td><td>Cen11</td><td>3-Oct-2018</td><td>21.8</td><td>FireAffected</td><td>SRR24677550</td><td>119434650</td><td>JBAIGG000000000</td><td>144335</td><td>345423017</td><td>2626</td><td>987027</td></tr><tr><td>Cen11_06102019</td><td>Cen11</td><td>6-Oct-2019</td><td>19.3</td><td>FireAffected</td><td>SRR24677616</td><td>68277302</td><td>JBAIGH000000000</td><td>82766</td><td>201039944</td><td>2701</td><td>855103</td></tr><tr><td>Cen11_13102020</td><td>Cen11</td><td>13-Oct-2020</td><td>19.5</td><td>FireAffected</td><td>SRR24677615</td><td>108943344</td><td>JBAIGI000000000</td><td>118956</td><td>238107306</td><td>2047</td><td>71568</td></tr><tr><td>Cen11_06102021</td><td>Cen11</td><td>6-Oct-2021</td><td>20</td><td>FireAffected</td><td>SRR24677614</td><td>93275172</td><td>JBAIGJ000000000</td><td>71500</td><td>151498571</td><td>2182</td><td>303453</td></tr><tr><td>Cen14_12102015</td><td>Cen14</td><td>12-Oct-2015</td><td>28.9</td><td>FireAffected</td><td>SRR24677613</td><td>123668420</td><td>JBAIGK000000000</td><td>117428</td><td>374847644</td><td>4394</td><td>901286</td></tr><tr><td>Cen14_11102016</td><td>Cen14</td><td>11-Oct-2016</td><td>29.4</td><td>FireAffected</td><td>SRR24677612</td><td>126966694</td><td>JBAIGL000000000</td><td>110577</td><td>403592710</td><td>6604</td><td>937175</td></tr><tr><td>Cen14_19102017</td><td>Cen14</td><td>19-Oct-2017</td><td>28.5</td><td>FireAffected</td><td>SRR24677611</td><td>122282866</td><td>JBAIGM000000000</td><td>121111</td><td>318405198</td><td>3155</td><td>717853</td></tr><tr><td>Cen14_02102018</td><td>Cen14</td><td>2-Oct-2018</td><td>30</td><td>FireAffected</td><td>SRR24677610</td><td>105414742</td><td>JBAIGN000000000</td><td>118460</td><td>387227442</td><td>4877</td><td>901280</td></tr><tr><td>Cen14_06102019</td><td>Cen14</td><td>6-Oct-2019</td><td>26.9</td><td>FireAffected</td><td>SRR24677609</td><td>101355352</td><td>JBAIGO000000000</td><td>123103</td><td>358796064</td><td>3771</td><td>883014</td></tr><tr><td>Cen14_13102020</td><td>Cen14</td><td>13-Oct-2020</td><td>25.4</td><td>FireAffected</td><td>SRR24677608</td><td>92846146</td><td>JBAIGP000000000</td><td>106684</td><td>262291366</td><td>2670</td><td>1293213</td></tr><tr><td>Cen14_05102021</td><td>Cen14</td><td>5-Oct-2021</td><td>22.1</td><td>FireAffected</td><td>SRR24677607</td><td>134497346</td><td>JBAIGQ000000000</td><td>97150</td><td>239260764</td><td>2798</td><td>1265966</td></tr><tr><td>Cen15_12102015</td><td>Cen15</td><td>12-Oct-2015</td><td>35.3</td><td>FireAffected</td><td>SRR24677605</td><td>125281422</td><td>JBAIGR000000000</td><td>128596</td><td>440403073</td><td>5157</td><td>1037399</td></tr><tr><td>Cen15_11102016</td><td>Cen15</td><td>11-Oct-2016</td><td>31.8</td><td>FireAffected</td><td>SRR24677604</td><td>153739606</td><td>JBAIGS000000000</td><td>120958</td><td>459008620</td><td>7193</td><td>960662</td></tr><tr><td>Cen15_19102017</td><td>Cen15</td><td>19-Oct-2017</td><td>27.2</td><td>FireAffected</td><td>SRR24677603</td><td>123055626</td><td>JBAIGT000000000</td><td>145031</td><td>425783382</td><td>3797</td><td>813692</td></tr><tr><td>Cen15_02102018</td><td>Cen15</td><td>2-Oct-2018</td><td>28.6</td><td>FireAffected</td><td>SRR24677602</td><td>137128144</td><td>JBAIGU000000000</td><td>144158</td><td>397881104</td><td>3459</td><td>643680</td></tr></tbody></table>
<table><tr><td>Cen15_06102019</td><td>Cen15</td><td>6-Oct-2019</td><td>25</td><td>FireAffected</td><td>SRR24677601</td><td>65267684</td><td>JBAIGV000000000</td><td>45047</td><td>112422555</td><td>2918</td><td>470205</td></tr><tr><td>Cen15_13102020</td><td>Cen15</td><td>13-Oct-2020</td><td>22.3</td><td>FireAffected</td><td>SRR24677600</td><td>115695690</td><td>JBAIGW000000000</td><td>113636</td><td>312812197</td><td>3463</td><td>2060246</td></tr><tr><td>Cen15_05102021</td><td>Cen15</td><td>5-Oct-2021</td><td>21.8</td><td>FireAffected</td><td>SRR24677599</td><td>108315540</td><td>JBAIGX000000000</td><td>99775</td><td>258547356</td><td>2858</td><td>915079</td></tr><tr><td>Cen16_12102015</td><td>Cen16</td><td>12-Oct-2015</td><td>22.4</td><td>FireAffected</td><td>SRR24677598</td><td>107303616</td><td>JBAIGY000000000</td><td>142748</td><td>347970221</td><td>2696</td><td>828568</td></tr><tr><td>Cen16_11102016</td><td>Cen16</td><td>11-Oct-2016</td><td>20.9</td><td>FireAffected</td><td>SRR24677597</td><td>113594820</td><td>JBAIGZ000000000</td><td>143271</td><td>351210329</td><td>2784</td><td>2423317</td></tr><tr><td>Cen16_19102017</td><td>Cen16</td><td>19-Oct-2017</td><td>18.9</td><td>FireAffected</td><td>SRR24677596</td><td>89626840</td><td>JBAIHA000000000</td><td>71367</td><td>203652565</td><td>3771</td><td>3243675</td></tr><tr><td>Cen16_02102018</td><td>Cen16</td><td>2-Oct-2018</td><td>22.2</td><td>FireAffected</td><td>SRR24677594</td><td>103256354</td><td>JBAIHB000000000</td><td>115981</td><td>279324043</td><td>2708</td><td>832243</td></tr><tr><td>Cen16_06102019</td><td>Cen16</td><td>6-Oct-2019</td><td>17.1</td><td>FireAffected</td><td>SRR24677593</td><td>98261786</td><td>JBAIHC000000000</td><td>147813</td><td>377442901</td><td>2928</td><td>2264022</td></tr><tr><td>Cen16_15102020</td><td>Cen16</td><td>15-Oct-2020</td><td>14.4</td><td>FireAffected</td><td>SRR24677592</td><td>99322676</td><td>JBAIHD000000000</td><td>107954</td><td>282993089</td><td>3109</td><td>998537</td></tr><tr><td>Cen16_05102021</td><td>Cen16</td><td>5-Oct-2021</td><td>19.5</td><td>FireAffected</td><td>SRR24677591</td><td>124001218</td><td>JBAIHE000000000</td><td>142040</td><td>365981807</td><td>2963</td><td>2177591</td></tr><tr><td>Cen17_12102015</td><td>Cen17</td><td>12-Oct-2015</td><td>14.4</td><td>Reference</td><td>SRR24677590</td><td>75573920</td><td>JBAIHF000000000</td><td>32434</td><td>49409001</td><td>1458</td><td>43291</td></tr><tr><td>Cen17_12102016</td><td>Cen17</td><td>12-Oct-2016</td><td>13.2</td><td>Reference</td><td>SRR24677589</td><td>98667852</td><td>JBAIHG000000000</td><td>110909</td><td>208504520</td><td>1821</td><td>312323</td></tr><tr><td>Cen17_21102017</td><td>Cen17</td><td>21-Oct-2017</td><td>13.8</td><td>Reference</td><td>SRR24677588</td><td>86602822</td><td>JBAIHH000000000</td><td>31726</td><td>51312983</td><td>1478</td><td>243625</td></tr><tr><td>Cen17_03102018</td><td>Cen17</td><td>3-Oct-2018</td><td>18</td><td>Reference</td><td>SRR24677587</td><td>96476092</td><td>JBAIHI000000000</td><td>25206</td><td>42231953</td><td>1526</td><td>134148</td></tr><tr><td>Cen17_06102019</td><td>Cen17</td><td>6-Oct-2019</td><td>15.1</td><td>Reference</td><td>SRR24677586</td><td>96267612</td><td>JBAIHJ000000000</td><td>52161</td><td>82649230</td><td>1517</td><td>53302</td></tr><tr><td>Cen17_14102020</td><td>Cen17</td><td>14-Oct-2020</td><td>12.5</td><td>Reference</td><td>SRR24677585</td><td>88243710</td><td>JBAIHK000000000</td><td>38196</td><td>63932648</td><td>1575</td><td>121107</td></tr><tr><td>Cen17_05102021</td><td>Cen17</td><td>5-Oct-2021</td><td>16.8</td><td>Reference</td><td>SRR24677583</td><td>50766062</td><td>JBAIHL000000000</td><td>18915</td><td>30147416</td><td>1502</td><td>66439</td></tr><tr><td>Cen19_12102015</td><td>Cen19</td><td>12-Oct-2015</td><td>31</td><td>FireAffected</td><td>SRR24677582</td><td>131714862</td><td>JBAIHM000000000</td><td>183930</td><td>558956168</td><td>4188</td><td>1562083</td></tr><tr><td>Cen19_11102016</td><td>Cen19</td><td>11-Oct-2016</td><td>46.3</td><td>FireAffected</td><td>SRR24677581</td><td>139131824</td><td>JBAIHN000000000</td><td>98344</td><td>387090322</td><td>7537</td><td>2346858</td></tr><tr><td>Cen19_19102017</td><td>Cen19</td><td>19-Oct-2017</td><td>41.3</td><td>FireAffected</td><td>SRR24677580</td><td>118895810</td><td>JBAIHO000000000</td><td>89093</td><td>324744972</td><td>6338</td><td>1496731</td></tr><tr><td>Cen19_02102018</td><td>Cen19</td><td>2-Oct-2018</td><td>27.3</td><td>FireAffected</td><td>SRR24677579</td><td>112603898</td><td>JBAIHP000000000</td><td>117000</td><td>378578881</td><td>5051</td><td>1690910</td></tr><tr><td>Cen19_06102019</td><td>Cen19</td><td>6-Oct-2019</td><td>23.7</td><td>FireAffected</td><td>SRR24677578</td><td>118770438</td><td>JBAIHQ000000000</td><td>118285</td><td>301729243</td><td>2814</td><td>1263664</td></tr><tr><td>Cen19_13102020</td><td>Cen19</td><td>13-Oct-2020</td><td>15.9</td><td>FireAffected</td><td>SRR24677577</td><td>92357358</td><td>JBAIHR000000000</td><td>102505</td><td>291470536</td><td>3685</td><td>1174055</td></tr><tr><td>Cen19_05102021</td><td>Cen19</td><td>5-Oct-2021</td><td>20.1</td><td>FireAffected</td><td>SRR24677576</td><td>103309696</td><td>JBAIHS000000000</td><td>120270</td><td>306633097</td><td>2938</td><td>797129</td></tr><tr><td>Cen21_12102015</td><td>Cen21</td><td>12-Oct-2015</td><td>33.8</td><td>FireAffected</td><td>SRR24677575</td><td>138522504</td><td>JBAIHT000000000</td><td>107426</td><td>419858096</td><td>7997</td><td>3014071</td></tr><tr><td>Cen21_11102016</td><td>Cen21</td><td>11-Oct-2016</td><td>37.3</td><td>FireAffected</td><td>SRR24677574</td><td>144438616</td><td>JBAIHU000000000</td><td>138350</td><td>531085627</td><td>7301</td><td>1234492</td></tr><tr><td>Cen21_19102017</td><td>Cen21</td><td>19-Oct-2017</td><td>31.5</td><td>FireAffected</td><td>SRR24677572</td><td>107447060</td><td>JBAIHV000000000</td><td>107230</td><td>383560699</td><td>6302</td><td>992864</td></tr><tr><td>Cen21_02102018</td><td>Cen21</td><td>2-Oct-2018</td><td>31.7</td><td>FireAffected</td><td>SRR24677571</td><td>123745008</td><td>JBAIHW000000000</td><td>131392</td><td>281481763</td><td>2161</td><td>1013129</td></tr></table>
<table><tr><td>Cen15_06102019</td><td>Cen15</td><td>6-Oct-2019</td><td>25</td><td>FireAffected</td><td>SRR24677601</td><td>65267684</td><td>JBAIGV000000000</td><td>45047</td><td>112422555</td><td>2918</td><td>470205</td></tr><tr><td>Cen15_13102020</td><td>Cen15</td><td>13-Oct-2020</td><td>22.3</td><td>FireAffected</td><td>SRR24677600</td><td>115695690</td><td>JBAIGW000000000</td><td>113636</td><td>312812197</td><td>3463</td><td>2060246</td></tr><tr><td>Cen15_05102021</td><td>Cen15</td><td>5-Oct-2021</td><td>21.8</td><td>FireAffected</td><td>SRR24677599</td><td>108315540</td><td>JBAIGX000000000</td><td>99775</td><td>258547356</td><td>2858</td><td>915079</td></tr><tr><td>Cen16_12102015</td><td>Cen16</td><td>12-Oct-2015</td><td>22.4</td><td>FireAffected</td><td>SRR24677598</td><td>107303616</td><td>JBAIGY000000000</td><td>142748</td><td>347970221</td><td>2696</td><td>828568</td></tr><tr><td>Cen16_11102016</td><td>Cen16</td><td>11-Oct-2016</td><td>20.9</td><td>FireAffected</td><td>SRR24677597</td><td>113594820</td><td>JBAIGZ000000000</td><td>143271</td><td>351210329</td><td>2784</td><td>2423317</td></tr><tr><td>Cen16_19102017</td><td>Cen16</td><td>19-Oct-2017</td><td>18.9</td><td>FireAffected</td><td>SRR24677596</td><td>89626840</td><td>JBAIHA000000000</td><td>71367</td><td>203652565</td><td>3771</td><td>3243675</td></tr><tr><td>Cen16_02102018</td><td>Cen16</td><td>2-Oct-2018</td><td>22.2</td><td>FireAffected</td><td>SRR24677594</td><td>103256354</td><td>JBAIHB000000000</td><td>115981</td><td>279324043</td><td>2708</td><td>832243</td></tr><tr><td>Cen16_06102019</td><td>Cen16</td><td>6-Oct-2019</td><td>17.1</td><td>FireAffected</td><td>SRR24677593</td><td>98261786</td><td>JBAIHC000000000</td><td>147813</td><td>377442901</td><td>2928</td><td>2264022</td></tr><tr><td>Cen16_15102020</td><td>Cen16</td><td>15-Oct-2020</td><td>14.4</td><td>FireAffected</td><td>SRR24677592</td><td>99322676</td><td>JBAIHD000000000</td><td>107954</td><td>282993089</td><td>3109</td><td>998537</td></tr><tr><td>Cen16_05102021</td><td>Cen16</td><td>5-Oct-2021</td><td>19.5</td><td>FireAffected</td><td>SRR24677591</td><td>124001218</td><td>JBAIHE000000000</td><td>142040</td><td>365981807</td><td>2963</td><td>2177591</td></tr><tr><td>Cen17_12102015</td><td>Cen17</td><td>12-Oct-2015</td><td>14.4</td><td>Reference</td><td>SRR24677590</td><td>75573920</td><td>JBAIHF000000000</td><td>32434</td><td>49409001</td><td>1458</td><td>43291</td></tr><tr><td>Cen17_12102016</td><td>Cen17</td><td>12-Oct-2016</td><td>13.2</td><td>Reference</td><td>SRR24677589</td><td>98667852</td><td>JBAIHG000000000</td><td>110909</td><td>208504520</td><td>1821</td><td>312323</td></tr><tr><td>Cen17_21102017</td><td>Cen17</td><td>21-Oct-2017</td><td>13.8</td><td>Reference</td><td>SRR24677588</td><td>86602822</td><td>JBAIHH000000000</td><td>31726</td><td>51312983</td><td>1478</td><td>243625</td></tr><tr><td>Cen17_03102018</td><td>Cen17</td><td>3-Oct-2018</td><td>18</td><td>Reference</td><td>SRR24677587</td><td>96476092</td><td>JBAIHI000000000</td><td>25206</td><td>42231953</td><td>1526</td><td>134148</td></tr><tr><td>Cen17_06102019</td><td>Cen17</td><td>6-Oct-2019</td><td>15.1</td><td>Reference</td><td>SRR24677586</td><td>96267612</td><td>JBAIHJ000000000</td><td>52161</td><td>82649230</td><td>1517</td><td>53302</td></tr><tr><td>Cen17_14102020</td><td>Cen17</td><td>14-Oct-2020</td><td>12.5</td><td>Reference</td><td>SRR24677585</td><td>88243710</td><td>JBAIHK000000000</td><td>38196</td><td>63932648</td><td>1575</td><td>121107</td></tr><tr><td>Cen17_05102021</td><td>Cen17</td><td>5-Oct-2021</td><td>16.8</td><td>Reference</td><td>SRR24677583</td><td>50766062</td><td>JBAIHL000000000</td><td>18915</td><td>30147416</td><td>1502</td><td>66439</td></tr><tr><td>Cen19_12102015</td><td>Cen19</td><td>12-Oct-2015</td><td>31</td><td>FireAffected</td><td>SRR24677582</td><td>131714862</td><td>JBAIHM000000000</td><td>183930</td><td>558956168</td><td>4188</td><td>1562083</td></tr><tr><td>Cen19_11102016</td><td>Cen19</td><td>11-Oct-2016</td><td>46.3</td><td>FireAffected</td><td>SRR24677581</td><td>139131824</td><td>JBAIHN000000000</td><td>98344</td><td>387090322</td><td>7537</td><td>2346858</td></tr><tr><td>Cen19_19102017</td><td>Cen19</td><td>19-Oct-2017</td><td>41.3</td><td>FireAffected</td><td>SRR24677580</td><td>118895810</td><td>JBAIHO000000000</td><td>89093</td><td>324744972</td><td>6338</td><td>1496731</td></tr><tr><td>Cen19_02102018</td><td>Cen19</td><td>2-Oct-2018</td><td>27.3</td><td>FireAffected</td><td>SRR24677579</td><td>112603898</td><td>JBAIHP000000000</td><td>117000</td><td>378578881</td><td>5051</td><td>1690910</td></tr><tr><td>Cen19_06102019</td><td>Cen19</td><td>6-Oct-2019</td><td>23.7</td><td>FireAffected</td><td>SRR24677578</td><td>118770438</td><td>JBAIHQ000000000</td><td>118285</td><td>301729243</td><td>2814</td><td>1263664</td></tr><tr><td>Cen19_13102020</td><td>Cen19</td><td>13-Oct-2020</td><td>15.9</td><td>FireAffected</td><td>SRR24677577</td><td>92357358</td><td>JBAIHR000000000</td><td>102505</td><td>291470536</td><td>3685</td><td>1174055</td></tr><tr><td>Cen19_05102021</td><td>Cen19</td><td>5-Oct-2021</td><td>20.1</td><td>FireAffected</td><td>SRR24677576</td><td>103309696</td><td>JBAIHS000000000</td><td>120270</td><td>306633097</td><td>2938</td><td>797129</td></tr><tr><td>Cen21_12102015</td><td>Cen21</td><td>12-Oct-2015</td><td>33.8</td><td>FireAffected</td><td>SRR24677575</td><td>138522504</td><td>JBAIHT000000000</td><td>107426</td><td>419858096</td><td>7997</td><td>3014071</td></tr><tr><td>Cen21_11102016</td><td>Cen21</td><td>11-Oct-2016</td><td>37.3</td><td>FireAffected</td><td>SRR24677574</td><td>144438616</td><td>JBAIHU000000000</td><td>138350</td><td>531085627</td><td>7301</td><td>1234492</td></tr><tr><td>Cen21_19102017</td><td>Cen21</td><td>19-Oct-2017</td><td>31.5</td><td>FireAffected</td><td>SRR24677572</td><td>107447060</td><td>JBAIHV000000000</td><td>107230</td><td>383560699</td><td>6302</td><td>992864</td></tr><tr><td>Cen21_02102018</td><td>Cen21</td><td>2-Oct-2018</td><td>31.7</td><td>FireAffected</td><td>SRR24677571</td><td>123745008</td><td>JBAIHW000000000</td><td>131392</td><td>281481763</td><td>2161</td><td>1013129</td></tr></table>
<table><tr><td>Cen21_06102019</td><td>Cen21</td><td>6-Oct-2019</td><td>30</td><td>FireAffected</td><td>SRR24677570</td><td>95991016</td><td>JBAIHX000000000</td><td>136882</td><td>358586810</td><td>2982</td><td>1789082</td></tr><tr><td>Cen21_13102020</td><td>Cen21</td><td>13-Oct-2020</td><td>24.5</td><td>FireAffected</td><td>SRR24677569</td><td>95674248</td><td>JBAIHY000000000</td><td>117940</td><td>299851161</td><td>2876</td><td>525405</td></tr><tr><td>Cen21_05102021</td><td>Cen21</td><td>5-Oct-2021</td><td>23.5</td><td>FireAffected</td><td>SRR24677568</td><td>149012682</td><td>JBAIHZ000000000</td><td>163041</td><td>414815233</td><td>2882</td><td>521355</td></tr><tr><td>Cen22_12102015</td><td>Cen22</td><td>12-Oct-2015</td><td>37.4</td><td>FireAffected</td><td>SRR24677567</td><td>172424074</td><td>JBAIIA000000000</td><td>85782</td><td>404428333</td><td>11958</td><td>1907411</td></tr><tr><td>Cen22_11102016</td><td>Cen22</td><td>11-Oct-2016</td><td>36.6</td><td>FireAffected</td><td>SRR24677566</td><td>134402064</td><td>JBAIIB000000000</td><td>108876</td><td>432565722</td><td>7259</td><td>2072426</td></tr><tr><td>Cen22_20102017</td><td>Cen22</td><td>20-Oct-2017</td><td>32.3</td><td>FireAffected</td><td>SRR24677565</td><td>143726066</td><td>JBAIIC000000000</td><td>163716</td><td>606439961</td><td>6325</td><td>2160330</td></tr><tr><td>Cen22_03102018</td><td>Cen22</td><td>3-Oct-2018</td><td>33.9</td><td>FireAffected</td><td>SRR24677564</td><td>127906794</td><td>JBAIID000000000</td><td>191865</td><td>585602205</td><td>4031</td><td>1469064</td></tr><tr><td>Cen22_06102019</td><td>Cen22</td><td>6-Oct-2019</td><td>30.8</td><td>FireAffected</td><td>SRR24677563</td><td>127426364</td><td>JBAIIE000000000</td><td>142582</td><td>488030302</td><td>5126</td><td>1690897</td></tr><tr><td>Cen22_13102020</td><td>Cen22</td><td>13-Oct-2020</td><td>32.5</td><td>FireAffected</td><td>SRR24677561</td><td>112687806</td><td>JBAIIF000000000</td><td>152990</td><td>503652415</td><td>5174</td><td>2160791</td></tr><tr><td>Cen22_07102021</td><td>Cen22</td><td>7-Oct-2021</td><td>26.4</td><td>FireAffected</td><td>SRR24677560</td><td>109576490</td><td>JBAIIG000000000</td><td>152720</td><td>424686625</td><td>3493</td><td>1577924</td></tr><tr><td>Cen23_13102015</td><td>Cen23</td><td>13-Oct-2015</td><td>13.9</td><td>Reference</td><td>SRR24677559</td><td>97457822</td><td>JBAIIH000000000</td><td>71816</td><td>141042993</td><td>1858</td><td>352202</td></tr><tr><td>Cen23_12102016</td><td>Cen23</td><td>12-Oct-2016</td><td>13.1</td><td>Reference</td><td>SRR24677558</td><td>93880664</td><td>JBAIII000000000</td><td>116985</td><td>219169519</td><td>1842</td><td>1138925</td></tr><tr><td>Cen23_21102017</td><td>Cen23</td><td>21-Oct-2017</td><td>12.5</td><td>Reference</td><td>SRR24677557</td><td>94190168</td><td>JBAIJJ000000000</td><td>83044</td><td>156188699</td><td>1837</td><td>103517</td></tr><tr><td>Cen23_04102018</td><td>Cen23</td><td>4-Oct-2018</td><td>16.7</td><td>Reference</td><td>SRR24677556</td><td>47821322</td><td>JBAIIK000000000</td><td>19974</td><td>36773187</td><td>1825</td><td>44173</td></tr><tr><td>Cen23_07102019</td><td>Cen23</td><td>7-Oct-2019</td><td>15</td><td>Reference</td><td>SRR24677555</td><td>102127352</td><td>JBAIIL000000000</td><td>83503</td><td>153966893</td><td>1774</td><td>97317</td></tr><tr><td>Cen23_15102020</td><td>Cen23</td><td>15-Oct-2020</td><td>12.1</td><td>Reference</td><td>SRR24677554</td><td>74528594</td><td>JBAIIM000000000</td><td>52468</td><td>105979068</td><td>2003</td><td>89100</td></tr><tr><td>Cen23_06102021</td><td>Cen23</td><td>6-Oct-2021</td><td>16</td><td>Reference</td><td>SRR24677553</td><td>70046314</td><td>JBAIIN000000000</td><td>48122</td><td>88671983</td><td>1795</td><td>57462</td></tr></table>

100

<caption>**Table 1:** Metagenome info for all samples including read and assembly summaries and accessions. Number of reads passing quality filtering combines both forward and reversed reads. Contigs only include those at least 1000 bp long.</caption>
<table><tr><td>Cen21_06102019</td><td>Cen21</td><td>6-Oct-2019</td><td>30</td><td>FireAffected</td><td>SRR24677570</td><td>95991016</td><td>JBAIHX000000000</td><td>136882</td><td>358586810</td><td>2982</td><td>1789082</td></tr><tr><td>Cen21_13102020</td><td>Cen21</td><td>13-Oct-2020</td><td>24.5</td><td>FireAffected</td><td>SRR24677569</td><td>95674248</td><td>JBAIHY000000000</td><td>117940</td><td>299851161</td><td>2876</td><td>525405</td></tr><tr><td>Cen21_05102021</td><td>Cen21</td><td>5-Oct-2021</td><td>23.5</td><td>FireAffected</td><td>SRR24677568</td><td>149012682</td><td>JBAIHZ000000000</td><td>163041</td><td>414815233</td><td>2882</td><td>521355</td></tr><tr><td>Cen22_12102015</td><td>Cen22</td><td>12-Oct-2015</td><td>37.4</td><td>FireAffected</td><td>SRR24677567</td><td>172424074</td><td>JBAIIA000000000</td><td>85782</td><td>404428333</td><td>11958</td><td>1907411</td></tr><tr><td>Cen22_11102016</td><td>Cen22</td><td>11-Oct-2016</td><td>36.6</td><td>FireAffected</td><td>SRR24677566</td><td>134402064</td><td>JBAIIB000000000</td><td>108876</td><td>432565722</td><td>7259</td><td>2072426</td></tr><tr><td>Cen22_20102017</td><td>Cen22</td><td>20-Oct-2017</td><td>32.3</td><td>FireAffected</td><td>SRR24677565</td><td>143726066</td><td>JBAIIC000000000</td><td>163716</td><td>606439961</td><td>6325</td><td>2160330</td></tr><tr><td>Cen22_03102018</td><td>Cen22</td><td>3-Oct-2018</td><td>33.9</td><td>FireAffected</td><td>SRR24677564</td><td>127906794</td><td>JBAIID000000000</td><td>191865</td><td>585602205</td><td>4031</td><td>1469064</td></tr><tr><td>Cen22_06102019</td><td>Cen22</td><td>6-Oct-2019</td><td>30.8</td><td>FireAffected</td><td>SRR24677563</td><td>127426364</td><td>JBAIIE000000000</td><td>142582</td><td>488030302</td><td>5126</td><td>1690897</td></tr><tr><td>Cen22_13102020</td><td>Cen22</td><td>13-Oct-2020</td><td>32.5</td><td>FireAffected</td><td>SRR24677561</td><td>112687806</td><td>JBAIIF000000000</td><td>152990</td><td>503652415</td><td>5174</td><td>2160791</td></tr><tr><td>Cen22_07102021</td><td>Cen22</td><td>7-Oct-2021</td><td>26.4</td><td>FireAffected</td><td>SRR24677560</td><td>109576490</td><td>JBAIIG000000000</td><td>152720</td><td>424686625</td><td>3493</td><td>1577924</td></tr><tr><td>Cen23_13102015</td><td>Cen23</td><td>13-Oct-2015</td><td>13.9</td><td>Reference</td><td>SRR24677559</td><td>97457822</td><td>JBAIIH000000000</td><td>71816</td><td>141042993</td><td>1858</td><td>352202</td></tr><tr><td>Cen23_12102016</td><td>Cen23</td><td>12-Oct-2016</td><td>13.1</td><td>Reference</td><td>SRR24677558</td><td>93880664</td><td>JBAIII000000000</td><td>116985</td><td>219169519</td><td>1842</td><td>1138925</td></tr><tr><td>Cen23_21102017</td><td>Cen23</td><td>21-Oct-2017</td><td>12.5</td><td>Reference</td><td>SRR24677557</td><td>94190168</td><td>JBAIJJ000000000</td><td>83044</td><td>156188699</td><td>1837</td><td>103517</td></tr><tr><td>Cen23_04102018</td><td>Cen23</td><td>4-Oct-2018</td><td>16.7</td><td>Reference</td><td>SRR24677556</td><td>47821322</td><td>JBAIIK000000000</td><td>19974</td><td>36773187</td><td>1825</td><td>44173</td></tr><tr><td>Cen23_07102019</td><td>Cen23</td><td>7-Oct-2019</td><td>15</td><td>Reference</td><td>SRR24677555</td><td>102127352</td><td>JBAIIL000000000</td><td>83503</td><td>153966893</td><td>1774</td><td>97317</td></tr><tr><td>Cen23_15102020</td><td>Cen23</td><td>15-Oct-2020</td><td>12.1</td><td>Reference</td><td>SRR24677554</td><td>74528594</td><td>JBAIIM000000000</td><td>52468</td><td>105979068</td><td>2003</td><td>89100</td></tr><tr><td>Cen23_06102021</td><td>Cen23</td><td>6-Oct-2021</td><td>16</td><td>Reference</td><td>SRR24677553</td><td>70046314</td><td>JBAIIN000000000</td><td>48122</td><td>88671983</td><td>1795</td><td>57462</td></tr></table>

100

<caption>**Table 1:** Metagenome info for all samples including read and assembly summaries and accessions. Number of reads passing quality filtering combines both forward and reversed reads. Contigs only include those at least 1000 bp long.</caption>
<figure><img src="image_1.png" /><figcaption>**Figure 1:** Contributions of the topmost represented phyla to the pool of contigs classified to the phylum level. Contribution is defined as the percent of the classified contigs. Phyla not making up at least 1% of the contigs in any sample are combined as "less than 1%". White lines indicate soil temperature at sampling (secondary y axis).</figcaption></figure>
<figure><img src="image_2.png" /><figcaption>**Figure 1:** Contributions of the topmost represented phyla to the pool of contigs classified to the phylum level. Contribution is defined as the percent of the classified contigs. Phyla not making up at least 1% of the contigs in any sample are combined as "less than 1%". White lines indicate soil temperature at sampling (secondary y axis).</figcaption></figure>