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GDR 3545
G Protein-coupled Receptors
From physiology to drugs
(RCPG-Physio-Med)

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4th Annual Meeting
November 4-6, 2015
Toulouse, France

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# Stimulation of olfactory receptors expressed in cancer cells promotes their invasiveness and proloferation, leading to increased metastasis emergence in vivo

Guenhaël Sanz, Isabelle Leray, Denise Grebert, Sharmilee Antoine, Adrien Acquistapace, Abdelhak Boukadiri, Lluis M. Mir

► To cite this version:

Guenhaël Sanz, Isabelle Leray, Denise Grebert, Sharmilee Antoine, Adrien Acquistapace, et al. Stimulation of olfactory receptors expressed in cancer cells promotes their invasiveness and proloferation, leading to increased metastasis emergence in vivo. 4. Annual Meeting GDR 3545 G Protein-coupled Receptors From physiology to drugs (RCPG-Physio-Med), Groupement de Recherche (GDR3545). FRA., Nov 2015, Toulouse, France. pp.138. hal-02738961

HAL Id: hal-02738961

https://hal.inrae.fr/hal-02738961

Submitted on 2 Jun 2020

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Distributed under a Creative Commons Attribution 4.0 International License
Lecture 12

Stimulation of olfactory receptors expressed in cancer cells promotes their invasiveness and proliferation, leading to increased metastasis emergence in vivo

Sanz Guenhaël¹, Leray Isabelle², Grébert Denise¹, Antoine Sharmilee¹, Acquistapace Adrien¹, Boukadiri Abdelhak³, Mir Lluis M.²

¹INRA UR1197 Unité de Neurobiologie de l'olfaction Jouy-en-Josas France, ²CNRS Gustave Roussy UMR8203 Laboratoire de Vectorologie et thérapeutiques anti-cancéreuses Villejuif France, ³INRA UMR 1313 Génétique Animale et Biologie Intégrative Jouy-en-Josas France

Olfactory receptors (ORs) are G protein-coupled receptors mainly expressed in the olfactory epithelium where they allow the detection and discrimination of a myriad of odorant molecules. Moreover, ORs are expressed in other tissues such as in some cancer cells, with additional functions. For instance, the OR51E2 olfactory receptor, also named prostate specific G protein-coupled receptor (PSGR), is overexpressed in prostate cancer cells and is considered a tumor marker of prostate cancer. We demonstrated its involvement in tumor progression: we showed that the PSGR is able to promote LNCaP prostate cancer cell invasiveness in vitro and in vivo upon stimulation with its odorant agonist β-ionone (G. Sanz et al, 9(1): e85110. 2014). We report now that a relatively short exposure to β-ionone (two weeks) is already able to promote metastasis emergence while a longer treatment exacerbates this committed effect. We also report that α-ionone, usually considered as an OR51E2 antagonist, promotes LNCaP tumor growth in vivo. Moreover, the combination of α-ionone with β-ionone displays a higher effect on tumor growth than each molecule alone. In vitro assessment of LNCaP cell growth upon exposure to α-ionone further demonstrates that α-ionone is not an OR51E2 antagonist as previously reported and supports the in vivo results. Altogether, our results show that α-ionone and β-ionone induce different cellular effects, each leading to increased tumor aggressiveness.
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## Preliminary Program

### Wednesday november 4

- 12:30 Registration

- 14:00 Welcome

#### Session 1 - GPCR regulation and signaling (Chairperson Lionel Moulédous)

- 14:15 **Ulla Petaja-Repo, University of Oulu (Finland)**
  GPCR folding, maturation and trafficking

- 15:00 **Andrew Tobin, University of Leicester (UK)**
  Cellular and physiological impact of GPCR phosphorylation

- 15:45 Coffee break

- 16:15 **Franck Vandermoere, IGF Université de Montpellier (France)**
  Quantitative phosphoproteomics unravels biased phosphorylation of serotonin 5-HT₂A receptor at Ser<sup>280</sup> by hallucinogenic versus non-hallucinogenic agonists

- 16:45 **Young Scientists selected from posters** (5)

- 18:30-21:00 **Poster Session 1**

- 19:00-22:00 Buffet

### Thursday november 5

#### Session 2 - Nanoscale organization and dynamics of GPCR signaling (Chairperson Laurence Salomé)

- 9:00 **Jörg Standfuss, Paul Scherrer Institute (Switzerland)**
  Molecular mechanisms of phosphorylation-dependent arrestin activation

- 9:45 **Julie Perroy, IGF Université de Montpellier (France)**
  Organization and dynamics of the glutamatergic receptosome at the synapse

- 10:30 Coffee break

- 11:00 **Céline Galès, I2MC Université de Toulouse (France)**
  AFM/adrenergic receptors

- 11:30 **Young Scientists selected from posters** (2)
12:00 Buffet

## Session 3 - GPCR targeting in pathophysiology

### Special Session: GPCR targeting in atherothrombosis and bleeding (Chairperson Jean-Michel Sénard)

13:30 Stuart Mundell, University of Bristol (UK)
Platelet dysfunction associated with GPCR variants in human

14:15 Kenneth Jacobson, NIH Bethesda (USA)
Structure and pharmacochemistry of purinergic receptors

15:00 Thibault Lhermusier, CHU Toulouse (France)
Benefits and risks of new antiplatelet agents

15:30 Coffee break

(Chairperson Daniel Fourmy)

16:00 Bernard Lagane, Institut Pasteur Paris (France)
Mechanisms of HIV-1 escape/resistance to inhibition by CCR5 ligands

16:45 Jean-Claude Reubi, University of Berne (Switzerland)
Regulatory peptide receptors as targets for tumor diagnosis and therapy

17:15 Guenhael Sanz, INRA Jouy en Josas (France)
Stimulation of olfactory receptors expressed in cancer cells promotes their invasiveness and proliferation, leading to increased metastasis emergence in vivo

17:45 Young Scientists selected from posters (3)

18:30 General Assembly of GDR members

19:30-22:00 Poster Session 2

20:00-22:00 Dinner

## Friday november 6

## Session 4 - Drug-discovery and genetic approaches to address GPCR function (Chairperson Alain Milon)

9:00 MatthiasTschöp, University of Munich (Germany)
A rationally designed monomeric peptide triagonist corrects obesity and diabetes in rodents

9:45 Gregg Siegal, ZoBio Leidein (The Netherlands)
Fragment screening of GPCRs using biophysical methods

10:30 Coffee break

11:00 Frédéric Simonin, ESBS Université de Strasbourg (France)
Genetic models for the study of RFamide receptors

11:30 Young Scientists selected from posters (2)

12:00 Ralf Jockers, Institut Cochin Université Descartes Paris (France)
Therapeutic potential of orphan GPCR

12:45-13:30 Awards and Conclusion