# Metagenome-scale metabolic modelling for the characterization of cross-feeding interactions in *Microcystis*-associated microbial communities, in the context of freshwater cyanobacterial blooms

Juliette AUDEMARD¹,², Sébastien HALARY³, Gabriel MARKOV⁴, Jeanne GOT⁵, Anne SIEGEL⁵, Marie LEFEBVRE¹, Julie LELOUP⁶
Benjamin MARIE³, Nicolas CREUSOT⁷, Binta DIEME¹, Clémence FRIOUX²

¹UMR 1019 INRAE-UCA MetaboHub, Clermont-Ferrand, France ²Inria, Univ. Bordeaux, INRAE, Talence, France ³UMR 7245 CNRS-MNHN, Paris, France ⁴UMR 8227, Sorbonne Univ., Station Biologique de Roscoff, France
⁵Université de Rennes, Inria, CNRS, IRISA, Rennes, France ⁶UMR 7618 CNRS-INRAE-IRD-UPEC Sorbonne Univ., Paris, France ⁷UMR1454 INRAE EABX MetaboHub, Univ. Bordeaux, Gazinet Cestas, France

<figure><img src="image_1.png" /></figure>

Inria
INRAE

UNIVERSITÉ
Clermont
Auvergne

<figure><img src="image_2.png" /></figure>

CNRS • SORBONNE UNIVERSITÉ
Station Biologique
de Roscoff

MUSÉUM
NAL HIST
NATURELLE

<figure><img src="image_3.png" /></figure>

Université
Paris Cité

[1] Ibelings et al. 'Cyanobacterial blooms. Ecology, prevention, mitigation and control: Editorial to a CYANOCOST Special Issue', Aquat Ecol, 2016.
[2] Louati, et al. 'The phyto-bacterioplankton couple in a shallow freshwater ecosystem: Who leads the dance?', Harmful Algae, 2023.
[3] Karp, et al. 'The Pathway Tools software', Bioinformatics, 2022.
[4] Belcour, et al. 'Metage2Metabo, microbiota-scale metabolic complementarity for the identification of key species', eLife, 2020.
[5] Caspi et al., 'The MetaCyc database of metabolic pathways and enzymes and the BioCyc collection of Pathway/Genome Databases', Nucleic Acids Research, 2014.
[6] Frioux, et al. 'Scalable and exhaustive screening of metabolic functions carried out by microbial consortia', Bioinformatics, 2018.
[7] Wang, et al. 'The inositol monophosphatase all2917 (IMPA1) is involved in osmotic adaptation in anabaena sp. PCC7120' Environmental Microbiology Reports, 2012.
[8] Zhang, et al. 'Characterization of typical taste and odor compounds formed by Microcystis aeruginosa', Journal of Environmental Sciences, 2013.
[9] Giordano et al. 'Nitrogen and sulfur assimilation in plants and algae', Aquatic Botany, 2014.