# Effects of Exercise Training on Anabolic and Catabolic Hormones with Advanced Age: A Systematic Review

Hassane Zouhal, Ayyappan Jayavel, Kamalanathan Parasuraman, Lawrence

D. Hayes, Claire Tourny, Fatma Rhibi, Ismail Laher, Abderraouf Ben

Abderrahman, Anthony C. Hackney

► To cite this version:

Hassane Zouhal, Ayyappan Jayavel, Kamalanathan Parasuraman, Lawrence D. Hayes, Claire Tourny, et al.. Effects of Exercise Training on Anabolic and Catabolic Hormones with Advanced Age: A Systematic Review. Sports Medicine, 2022, 52, pp.1353-1368. 10.1007/s40279-021-01612-9. hal-03512830

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# Effects of Exercise Training on Anabolic and Catabolic Hormones with Advanced Age: A Systematic Review

Hassane Zouhal¹,² ID · Ayyappan Jayavel³ · Kamalanathan Parasuraman³ · Lawrence D. Hayes⁴ · Claire Tourny⁵ · Fatma Rhibi¹ · Ismail Laher⁶ · Abderraouf Ben Abderrahman⁷ · Anthony C. Hackney⁸

Accepted: 21 November 2021

© Crown 2021

## Abstract

**Background** Ageing is accompanied by decreases in physical capacity and physiological regulatory mechanisms including altered hormonal regulation compared with age-matched sedentary people. The potential benefits of exercise in restoring such altered hormone production and secretion compared to age-matched physically inactive individuals who are ageing remains unclear.

**Objectives** The aim of this systematic review was to summarise the findings of exercise training in modulating levels of ostensibly anabolic and catabolic hormones in adults aged > 40 years.

**Methods** We searched the following electronic databases (to July 2021) without a period limit: Cochrane Library, PubMed, Science Direct, Scopus, SPORTDiscus and Web of Science. Additionally, a manual search for published studies in Google Scholar was conducted for analysis of the 'grey literature' (information produced outside of traditional commercial or academic publishing and distribution channels). The initial search used the terms 'ageing' OR 'advanced age' OR 'old people' OR 'older' OR elderly' AND 'anabolic hormones' OR 'catabolic hormones' OR 'steroid hormones' OR 'sex hormones' OR 'testosterone' OR 'cortisol' OR 'insulin' OR 'insulin-like growth factor-1' OR 'IGF-1' OR 'sex hormone-binding globulin' OR 'SHBG' OR 'growth hormone' OR 'hGH' OR 'dehydroepiandrosterone' OR 'DHEA' OR 'dehydroepiandrosterone sulfate (DHEA-S)' AND 'exercise training' OR 'endurance training' OR 'resistance training' OR 'strength training' OR 'weight-lifting' OR 'high-intensity interval training' OR 'high-intensity interval exercise' OR 'high-intensity intermittent training' OR 'high-intensity intermittent exercise' OR 'interval aerobic training' OR 'interval aerobic exercise' OR 'intermittent aerobic training' OR 'intermittent aerobic exercise' OR 'high-intensity training' OR 'high-intensity exercise' OR 'sprint interval training' OR 'sprint interval exercise' OR 'combined exercise training' OR 'anaerobic training'. Only eligible full texts in English or French were considered for analysis.

**Results** Our search identified 484 records, which led to 33 studies for inclusion in the analysis. Different exercise training programs were used with nine studies using endurance training programs, ten studies examining the effects of high-intensity interval training, and 14 studies investigating the effects of resistance training. Most training programs lasted ≥ 2 weeks. Studies, regardless of the design, duration or intensity of exercise training, reported increases in testosterone, sex hormone-binding globulin (SHBG), insulin-like growth factor-1 (IGF-1), human growth hormone (hGH) or dehydroepiandrosterone (DHEA) (effect size: 0.19 < *d* < 3.37, small to very large) in both older males and females. However, there was no consensus on the effects of exercise on changes in cortisol and insulin in older adults.

**Conclusion** In conclusion, findings from this systematic review suggest that exercise training increases basal levels of testosterone, IGF-1, SHBG, hGH and DHEA in both male and females over 40 years of age. The increases in blood levels of these hormones were independent of the mode, duration and intensity of the training programs. However, the effects of long-term exercise training on cortisol and insulin levels in elderly people are less clear.

## 1 Introduction

Abderraouf Ben Abderrahman and Anthony C. Hackney are equal last authors.

Extended author information available on the last page of the article

Ageing is characterized by decreases in physical capacity that are related to a loss of muscle mass and decreased muscle contraction velocity [1] and maximum strength, which
## Key Points

For basal concentrations of ostensibly anabolic hormones, exercise training produced trivial to very large increases in testosterone, small to moderate increases in insulin-like growth factor-1 (IGF-1), small to very large increases in human growth hormone (hGH), trivial to very large increases in insulin, and small to large increases in dehydroepiandrosterone sulphate (DHEA-S) in adults aged > 40 years.

Small to large increases were observed for basal sex hormone-binding globulin (SHBG).

For the ostensibly catabolic hormone cortisol, effects ranged from a small decrease to a very large increase. Moderate effects and large effects were observed for the other corticosteroids cortisone and corticosterone, respectively.

Observed alterations to the hormonal mileu were not readily related to participant age or acute training programme variables, or chronic training variables (e.g. intensity, volume, frequency).

accelerate in particular after 50 years of age [2, 3]. According to Korhonen et al. [4], strength and muscle volume peaks around ~30 years of age and decreases by 15% per decade from age 50 onwards, until 70 years of age, when strength is ~30% of that of a 30-year-old [5, 6]. This decrease in muscle strength is due in large part to decreases in the contractile properties of muscle [5, 7], especially in the number of fast-twitch type II muscle fibers [5, 8, 9].

Loss of physical function and hormonal status are important determinants of health and longevity in older adults [10–13]. Concentrations of anabolic hormones are altered with advancing age, as shown by yearly 1% decreases in testosterone in about 50% of men ≥ 30 years of age (from peak values at 20 years of age) [14], suggesting changes in anabolic hormones start from the fourth decade of life [15, 16]. The fall in blood testosterone levels during ageing diminishes its anabolic effects in skeletal muscle, and so negatively impacts neuromuscular performance [17], muscle mass and bone mineral density [18].

Ageing alters metabolism and degradation of hormones, especially in those individuals with decreased liver or kidney function [19]. In addition, ageing reduces target cell hormone receptor number, affinity and signal transduction [20, 21]. Moreover, anabolic hormones such as testosterone inhibit the secretion of cortisol, diminishing glucocorticoid-mediated catabolism of skeletal muscle, meaning

testosterone has both anabolic and anti-catabolic effects [22–24]. Nearly 98% of circulating testosterone is bound to sex hormone-binding globulin (SHBG) and albumin [25], both of which are also altered by the ageing process [26]. As the concentration of SHBG increases with age, the level of free (unbound) and bioavailable (bound loosely to albumin) testosterone decreases, so that there is less testosterone available for tissue uptake [27]. Interestingly, there are similar patterns of androgenic hormone decline (andropause) and somatotropic hormone decline (somatopause); the latter also have anabolic actions [14]. Specifically, human growth hormone (hGH) and its main downstream protein, insulin-like growth factor-1 (IGF-1), decrease with advanced age [14]. Secretion of hGH decreases by ~14% per decade after 20 years of age [14], and reaches, by the age of ~60 years, half of the hGH secretion of younger counterparts (20–30 years) [28]. The main stimulated protein downstream of hGH, IGF-1, as already noted, also decreases with age (~10% per decade) [28]. IGF-1 is also anabolic in nature, increases cell proliferation, cell differentiation and energy metabolism, and prevents apoptosis [29].

Although endocrine dysregulation is associated with advanced age, it is difficult to attribute this alteration to age exclusively, as physical activity and exercise also influence the hormonal mileu [19]. Age is associated with physical inactivity [30], which can influence the age-endocrine dysregulation relationship. While ageing per se may not cause endocrinological dysregulation, age-associated increases in sedentary behaviour could [31–34]. Exercise is a non-pharmacological strategy to counteract come of the physiological changes that occur with age, including endocrine changes [19, 35–40].

Exercise exerts well-known health-promoting cardioprotective effects [41], with recent meta-analytical evidence demonstrating running activities were associated with a 30% reduction in cardiovascular mortality [42]. This emphasizes the importance of physical activity for health, supporting the recent narrative by the UK government that identified a curvilinear dose-response relationship between physical activity and health outcomes [43]. Moreover, several reports or opinion pieces suggest exercise may be a countermeasure to human biological ageing [44]. Thus, exercise and physical activity ameliorate many deleterious effects of chronological ageing on multiple physiological systems. There are some reports that lifelong exercisers are more phenotypically younger in terms of endocrinological profile than their sedentary counterparts, and exercise interventions result in a 'younger' hormonal profile than before undertaking exercise [45]. Therefore, it appears consistent physical exercise may be required to maintain endocrine function with ageing. However, before exercise can be proposed as a viable countermeasure to endocrinological dysregulation, it is important to consider the existing literature in terms of methodologies,
quality of research and heterogeneity, and conduct a systematic review of available literature. To the best of our knowledge, only one narrative review [19] and two book chapters [46, 47] have reviewed the effects of physical exercise on changes in anabolic and catabolic hormones in older adults. Therefore, it seemed prudent to conduct a systematic review of the effects of various exercise training protocols on ostensibly anabolic and catabolic hormones in people aged > 40 years with normal body mass.

## 2 Methods

### 2.1 Eligibility Criteria

Population, Intervention, Comparison, Outcome and Study design (PICOS) criteria were used for inclusion of studies in this review (see Table 1) [48]. This systematic review included original studies (randomized or non-randomized) for which the full texts were available and that performed interventions with exercise training, included 2 or more weeks of follow-up, and involved subjects who were aged between 40 and 85 years. We included studies that involved one or both sexes, and specifically evaluated blood levels of any of the following hormones: total testosterone, cortisol, insulin, IGF-1, SHBG, hGH, dehydroepiandrosterone (DHEA) and dehydroepiandrosterone sulfate (DHEA-S) before and after exercise.

Duplicate publications or sub-topics of included studies [e.g., studies involving co-morbidities or pathologies, and studies linking exercise to nutritional interventions (e.g., nutrition counselling, balanced or hypocaloric diets, and supplements)] or pharmacological agents were all excluded to reduce confounding factors [49]. Studies involving individuals with overweight or obese BMIs (BMI ≥ 25 kg/m²] were also excluded [49].

### 2.2 Literature Search Strategy

This systematic review is reported in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement and the Cochrane Handbook for Systematic Reviews of Interventions [50]. The study

protocol was registered (CRD42019138269) in the International Prospective Register of Systematic Reviews (PROSPERO) platform.

We searched the following electronic databases (to July 2021) without a period limit: Cochrane Library, PubMed, Science Direct, Scopus, SPORTDiscus and Web of Science. Additionally, a manual search for published studies in Google Scholar was conducted for analysis of the 'grey literature' (information produced outside of traditional commercial or academic publishing and distribution channels). The initial search used the terms 'ageing' OR 'advanced age' OR 'old people' OR 'older' OR elderly' AND 'anabolic hormones' OR 'catabolic hormones' OR 'steroid hormones' OR 'sex hormones' OR 'testosterone' OR 'cortisol' OR 'insulin' OR 'insulin-like growth factor-1' OR 'IGF-1' OR 'sex hormone-binding globulin' OR 'SHBG' OR 'growth hormone' OR 'hGH' OR 'dehydroepiandrosterone' OR 'DHEA' OR 'dehydroepiandrosterone sulfate (DHEA-S)' AND 'exercise training' OR 'endurance training' OR 'resistance training' OR 'strength training' OR 'weight-lifting' OR 'high-intensity interval training' OR 'high-intensity interval exercise' OR 'high-intensity intermittent training' OR 'high-intensity intermittent exercise' OR 'interval aerobic training' OR 'interval aerobic exercise' OR 'intermittent aerobic training' OR 'intermittent aerobic exercise' OR 'high-intensity training' OR 'high-intensity exercise' OR 'sprint interval training' OR 'sprint interval exercise' OR 'combined exercise training' OR 'anaerobic training'. Only eligible full texts in English or French were considered for analysis.

### 2.3 Study Selection and Data Extraction

Three authors independently performed searches in the electronic databases, and disagreements were resolved by consensus. The literature search strategies used for all databases are available in the supporting information.

The data-collection process is shown in Fig. 1 [51]. Titles and abstracts of selected articles were independently assessed by two researchers (HZ and AJ). The reviewers were not blinded to the authors, institutions or journals associated with the studies. Abstracts that provided insufficient information on inclusion and exclusion criteria were

<table><caption>**Table 1** PICOS (participants, interventions, comparisons, outcomes, study design)</caption><thead><tr><td>PICOS component</td><td>Details</td></tr></thead><tbody><tr><td>Participants</td><td>Healthy humans aged: > 40 and < 85 years</td></tr><tr><td>Interventions</td><td>Exercise training with two or more weeks of follow-up</td></tr><tr><td>Comparisons</td><td>Control group/Untrained participants</td></tr><tr><td>Outcomes</td><td>Physical performances, anabolic/catabolic hormone responses</td></tr><tr><td>Study designs</td><td>nRCTs, nRnCTs and RCTs</td></tr></tbody></table>

nRCT non-randomized controlled trial, nRnCT non-randomized non-controlled trial, RCT randomized controlled trial
retrieved for full-text analysis. Furthermore, the researchers independently analysed the full text and determined the eligibility of the studies, and disagreements were resolved by consensus. The agreement rate between the reviewers was 97% for the eligibility criteria of the study.

Corresponding authors of publications were contacted to avoid duplicate-counting of participants or to clarify questions about the methods where necessary. The corresponding authors were also contacted to provide data that may not have been included in the publications. Two researchers (HZ and AJ) independently performed the data extraction, and disagreements were resolved by consensus. Data were extracted for pre- and post-training hormone levels.

## 2.4 Assessment of Risk of Bias

The quality of studies was assessed using the Physiotherapy Evidence Database (PEDro) scale (http://www.pedro.

fhs.usyd.edu.au), which has good reliability and validity [52]. The PEDro scale has 11 possible points that examine external validity (criterion 1) and internal validity (criteria 2–9) of controlled trials, and also enable determination of whether there was sufficient statistical information for interpreting results (criteria 10–11). The items of the scale are: (1) eligibility criteria were specified; (2) subjects were randomly allocated to groups; (3) allocation was concealed; (4) groups were similar at baseline; (5) subjects were blinded; (6) therapists who administered the treatment were blinded; (7) assessors were blinded; (8) measures of key outcomes were obtained from more than 85% of subjects; (9) data were analysed by intention to treat; (10) statistical comparisons between groups were conducted; and (11) point measures and measures of variability were provided. The first criterion is not included in the final score. Moreover, because of the nature of the physical activity interventions, where patient and therapy blinding and allocation are unlikely, the

<figure><img src="image_2.png" /><figcaption>**Fig. 1** Selection process for research articles (n=33) included in this systematic review. Adapted version of the recommendations in the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) statement [43]</figcaption></figure>
total score a trial could receive was limited to eight points. A cut-off of six points on the PEDro scale signified high-quality studies, as this has been reported to be sufficient to determine high-quality versus low-quality studies [52]. The studies were evaluated by two experienced investigators (HZ and AJ), and in the event of disagreement a third reviewer (ACH) was invited to further review the findings.

## 2.5 Data Analysis

The percent change (Δ%) was calculated (if not available in the study) for each study to evaluate the magnitude of the effects using the following equation:

$$ \Delta\% = (Mpost - Mpre)/Mpre \times 100 $$

where Mpost represents the mean value after (acute exercise or long-term of training) and Mpre represents the baseline mean value.

Effect sizes (ES) were computed to present standardized effects of acute and long-term training on the outcome variables (e.g., hormones and physical performance). The ES was calculated with Cohen's d [53] by dividing the raw ES (difference in means) by the pooled standard deviations:

$$ ES = (Cohen's d) = (M1 - M2)/SD pooled $$

Values for ES were defined as trivial (<0.2), small (0.2–0.6), moderate (0.6–1.2), large (1.2–2.0) and very large > 2 [54]. Results for each outcome variable are presented with number of observations (N), Δ% and ES. Data analysis was processed using SigmaStat 3.5 software (Systat, Inc, San Jose, CA, USA). The ES and Δ% were analysed in studies where sufficient data were available. A significant difference was indicated when the 95% confidence interval (CI) of the ES did not overlap zero.

# 3 Results

## 3.1 Study Selection

Our search identified 484 studies related to the effects of exercise on hormone levels in adults over 40 years of age (Fig. 1). After screening of titles, abstracts and full texts, 33 studies were selected for inclusion in our final analysis, and the characteristics of these long-term studies are summarised in Table 2. The 33 studies were carried out in different countries from five continents (Africa, North America, Europe, Asia and Australia). Fifteen studies investigated only male subjects, ten studied female subjects exclusively, while eight studies investigated both males and females.

A total of 2211 participants (age range = 40–85 years) underwent exercise-training programs and completed the

studies. The 33 studies used different exercise-training protocols, with nine studies using endurance-training programs, ten studies examining the effects of high-intensity interval training (HIIT) and 14 studies investigating the effects of resistance training. The training duration lasted at least 2 weeks [4], but several studies used ~12 weeks, and four studies used a 52-week exercise protocol [55, 56]. Studies were generally classified as 'high-quality' studies (mean 6.9 in the PEDro scale score) (Table 3).

## 3.2 Total Testosterone

Twelve studies investigated the effects of training on testosterone concentrations in older adults [16, 57–67] (Table 4). Irrespective of the exercise protocol (type of exercise, duration or intensity of exercise training), these studies all reported increases in basal total testosterone in both male and female participants (effect size: 0.19 < d < 3.37, small to very large).

## 3.3 Cortisol

Nine studies investigated the effects of exercise training on cortisol in older adults (Table 5). Six studies reported increases in basal cortisol concentrations [15, 16, 58, 64, 67, 68] (effect size: 0.27 < d < 2.69, small to very large), while three studies observed decreases [62, 63, 69] (effect size: 0.27 < d < 0.46, small).

## 3.4 Insulin

The effects of long-term training on insulin concentrations in older adults are summarised in Table 6. Nine studies [15, 64, 65, 70–74] reported decreased basal insulin (effect size: 0.04 < d < 2.30, small to very large) and four studies [4, 69, 75, 76] observed increased concentrations (effect size: 0.32 < d < 0.56, small).

## 3.5 Insulin-Like Growth Factor-1 (IGF-1)

Nine studies investigated the effects of training on IGF-1 concentrations in older adults (Table 7). Among these, eight studies reported significant increases in IGF-1 in both elderly males and females (effect size: 0.27 < d < 1.03, small to moderate) [65, 66, 69, 72, 77–80] and one study reported decreased IGF-1 [73] (effect size: 1.06).

## 3.6 Sex Hormone-Binding Globulin (SHBG)

Studies of the effects of training on SHBG concentrations in older adults are summarised in Table 8. Six studies [15, 55, 60–63] reported increases in basal SHBG concentrations in elderly women and men irrespective of the type, duration
<table><caption>**Table 2** Characteristics of studies that examined the effect of acute and chronic exercises on anabolic and catabolic hormones</caption><thead><tr><th>Study</th><th>Year</th><th>PEDro scale</th><th>Population/sex/ sample size</th><th>Sample size</th><th>Country</th><th>Age, years (mean ± SD) or age range</th><th>Characteristics of exercise training</th><th>Duration (weeks)</th></tr></thead><tbody><tr><td>Friedenreih et al. [69]</td><td>2019</td><td>8</td><td>Post-menopausal women</td><td>396</td><td>Canada</td><td>59.4 ± 4.9</td><td>Moderate and high-intensity training</td><td>52</td></tr><tr><td>Vaczi et al. [59]</td><td>2014</td><td>7</td><td>Older men</td><td>16</td><td>Hungary</td><td>65.7 ± 5.3</td><td>Stretch shortening cycle and eccentric training</td><td>10</td></tr><tr><td>Im et al. [10]</td><td>2019</td><td>7</td><td>Older women</td><td>25</td><td>Korea</td><td>69.4 ± 2.9</td><td>Yoga and Korean dance</td><td>12</td></tr><tr><td>Søgaard et al. [77]</td><td>2018</td><td>6</td><td>Older men and women</td><td>22</td><td>Denmark</td><td>63 ± 1</td><td>High-intensity interval training</td><td>6</td></tr><tr><td>Ahtiainen et al. [60]</td><td>2011</td><td>7</td><td>Older men</td><td>35</td><td>Finland</td><td>61 ± 5</td><td>Heavy resistance exercise</td><td>21</td></tr><tr><td>Ahtiainen et al. [61]</td><td>2015</td><td>7</td><td>Older men</td><td>13</td><td>Finland</td><td>70 ± 2</td><td>Heavy resistance exercise</td><td>52</td></tr><tr><td>Banitalebi et al. [70]</td><td>2018</td><td>9</td><td>Older women</td><td>48</td><td>Iran</td><td>67.4 ± 1.4</td><td>Resistance and endurance training</td><td>12</td></tr><tr><td>Consitt et al. [76]</td><td>2016</td><td>7</td><td>Young and older</td><td>20</td><td>USA</td><td>19–29 and 57–82</td><td>Endurance and strength training</td><td>12</td></tr><tr><td>DiPietro et al. [63]</td><td>2008</td><td>7</td><td>Older women</td><td>20</td><td>USA</td><td>77 ± 6</td><td>Aerobic training and strength training</td><td>36</td></tr><tr><td>Glintborg et al. [62]</td><td>2013</td><td>10</td><td>Older males</td><td>54</td><td>Denmark</td><td>68</td><td>Strength training</td><td>12</td></tr><tr><td>Ha et al. [72]</td><td>2018</td><td>7</td><td>Older women</td><td>20</td><td>North Korea</td><td>73 ± 2.8</td><td>Combined resistance training and aerobic training</td><td>12</td></tr><tr><td>Hayes et al. [63]</td><td>2015</td><td>7</td><td>Older men</td><td>48</td><td>Scotland</td><td>61 ± 5</td><td>Low- to medium- and high-intensity training</td><td>6</td></tr><tr><td>Hayes et al. [90]</td><td>2015</td><td>6</td><td>Sedentary aged men</td><td>22</td><td>UK</td><td>62 ± 2</td><td>High-intensity training</td><td>6</td></tr><tr><td>Kim et al. [56]</td><td>2017</td><td>7</td><td>Older men and women</td><td>555</td><td>USA</td><td>51</td><td>Moderate physical activity</td><td>52</td></tr><tr><td>Krishnan et al. [65]</td><td>2013</td><td>7</td><td>Premenopausal women</td><td>28</td><td>USA</td><td>46.7 ± 3.3</td><td>Aerobic and resistance training</td><td>24</td></tr><tr><td>Micielska et al. [73]</td><td>2019</td><td>6</td><td>Healthy inactive women</td><td>33</td><td>Poland</td><td>45 ± 13</td><td>High-intensity circuit training</td><td>5</td></tr><tr><td>Motiani et al. [55]</td><td>2017</td><td>7</td><td>Sedentary men and women</td><td>26</td><td>Finland</td><td>45–55</td><td>Moderate-intensity interval training</td><td>2</td></tr><tr><td>Nunes et al. [66]</td><td>2019</td><td>7</td><td>Post-menopausal women</td><td>34</td><td>Brazil</td><td>64.2</td><td>Resistance training</td><td>16</td></tr><tr><td>Ogawa et al. [74]</td><td>2010</td><td>6</td><td>Older women</td><td>21</td><td>Japan</td><td>85.0 ± 4.5</td><td>Resistance training</td><td>12</td></tr><tr><td>De Guia et al. [94]</td><td>2019</td><td>6</td><td>Older men</td><td>43</td><td>Denmark</td><td>46.5 ± 3.0</td><td>Aerobic and resistance training</td><td>12</td></tr><tr><td>Praksch et al. [78]</td><td>2019</td><td>7</td><td>Older women</td><td>60</td><td>Hungary</td><td>67.4 ± 5</td><td>Home-based walking and aerobic training</td><td>12</td></tr><tr><td>Ramos et al. [75]</td><td>2016</td><td>7</td><td>Elderly men and women</td><td>66</td><td>Australia</td><td>58 ± 7</td><td>MICT and high-intensity training</td><td>16</td></tr><tr><td>Sato et al. [67]</td><td>2014</td><td>6</td><td>Older men</td><td>19</td><td>Japan</td><td>67.2 ± 1.8</td><td>Resistance training</td><td>12</td></tr><tr><td>Sellami et al. [15]</td><td>2016</td><td>7</td><td>Moderately trained late adult men</td><td>36</td><td>Tunisia</td><td>40.7 ± 1.8</td><td>Combined sprint and resistance training</td><td>13</td></tr></tbody></table>
<table><caption>**Table 2** (continued)</caption><thead><tr><th>Study</th><th>Year</th><th>PEDro scale</th><th>Population/sex/ sample size</th><th>Sample size</th><th>Country</th><th>Age, years (mean ± SD) or age range</th><th>Characteristics of exercise training</th><th>Duration (weeks)</th></tr></thead><tbody><tr><td>Sellami et al. [16]</td><td>2018</td><td>7</td><td>Moderately trained late adult men</td><td>40</td><td>Tunisia</td><td>40 ± 2</td><td>Combined sprint and resistance training</td><td>13</td></tr><tr><td>Walker et al. [68]</td><td>2015</td><td>7</td><td>Older men</td><td>18</td><td>USA</td><td>63.7 ± 3</td><td>Resistance training</td><td>20</td></tr><tr><td>Yamada et al. [79]</td><td>2015</td><td>7</td><td>Community-dwelling older men and women</td><td>222</td><td>Japan</td><td>76.3 ± 5.9</td><td>Walking exercise and nutrition</td><td>24</td></tr><tr><td>Bermon et al. [80]</td><td>1999</td><td>6</td><td>Sedentary and trained older adults</td><td>32</td><td>France</td><td>70.1 ± 1</td><td>Strength training</td><td>8</td></tr><tr><td>Bennefoy et al. [82]</td><td>1999</td><td>6</td><td>Community-dwelling older adults</td><td>32</td><td>France</td><td>69.7 ± 2.2</td><td>Physical activity</td><td>2</td></tr><tr><td>Craig et al. [58]</td><td>1989</td><td>6</td><td>Older men</td><td>9</td><td>USA</td><td>62.8 ± 0.7</td><td>Progressive resistance exercise</td><td>12</td></tr></tbody></table>

PEDro scale physiotherapy evidence database scale, F female, M male, M/F male and female, x times, W weeks, H hour, min minutes, Others other methods of intervention beyond the physical activity, BMI body mass index, SD standard deviation

and intensity of exercise training (effect size: 0.25 < d < 1.68, small to large).

## 3.7 Growth Hormone (hGH)

Only four studies investigated the effects of long-term training on basal hGH concentrations in older adults [67, 69, 73, 81] (Table 9). These studies reported increases in hGH in response to physical training (effect size: 0.29 < d < 2.58, small to very large).

## 3.8 Dehydroepiandrosterone (DHEA) and Dehydroepiandrosterone Sulfate (DHEA-S)

Studies examining the effects of long-term training on basal DHEA concentrations in older adults are summarised in Table 10. Six studies [10, 55, 64, 66, 78, 82] reported increases in DHEA in older males and females (effect size: 0.37 < d < 1.71, small to large). Only one study [65] reported a decrease (effect size: 0.28, small) in DHEA in post-menopausal women in response to 16 weeks of resistance training.

## 4 Discussion

This systematic review indicates that exercise training increases basal total testosterone, IGF-1, SHBG, hGH, DHEA and DHEA-S in males and females ≥ 40 years of age. Effects of exercise on blood hormones occurred regardless of the type, duration and intensity of training programs, with the exception of a lack of consensus on the effects of

long-term exercise training on cortisol and insulin responses in older adults (Fig. 2).

## 4.1 Effect of Exercise on Testosterone Levels

Exercise tended to produce small to large increases in total testosterone, supporting the supposition that exercise is the most convenient non-pharmacological means of increasing testosterone production and concomitantly preventing muscle loss in the elderly [60]. Different forms of exercise training can increase testosterone [60], although this finding is not ubiquitous [32, 83]. For example, 6 weeks of progressive resistance exercise increased muscle testosterone levels in the elderly, due to increased muscle steroidogenesis [66]. This corresponded to increased blood free testosterone, although total testosterone was not reported as commonly measured by other investigations, which may explain the divergency of results. Herbert et al. [33] previously reported that exercise increased free testosterone but not total testosterone levels [33]. Preconditioning exercise (10% increase) and HIIT (7% increase) combined to increase total testosterone by 17% in previously sedentary males, but only increased free testosterone by 5% [63]. Thus, the fraction of testosterone measured may result in different findings between studies, as supported by a recent meta-analysis of exercise-induced testosterone changes that concluded that free testosterone, rather than total testosterone, was more likely to change following resistance exercise [83].

The findings of this systematic review suggest exercise increases blood testosterone in both males and females, although several studies suggest men were more likely to benefit from exercise training in terms of increased total
<table><caption>**Table 3** Physiotherapy evidence database (PEDro) score of the included longitudinal studies</caption><thead><tr><th>Study</th><th>Year</th><th>Eligibility criteria</th><th>Randomized allocation</th><th>Blinded allocation</th><th>Group Homogeneity</th><th>Blinded subjects</th><th>Blinded therapists</th><th>Blinded assessor</th><th>Drop out ≥ 15%</th><th>Intention-to-treat analysis</th><th>Between-group comparison</th><th>Point estimates and variability</th><th>PEDro sum</th></tr></thead><tbody><tr><td><strong>Aerobic—endurance training</strong></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>Bennefoy et al. [82]</td><td>1999</td><td>●</td><td>○</td><td>○</td><td>●</td><td>○</td><td>○</td><td>●</td><td>●</td><td>○</td><td>●</td><td>●</td><td>6</td></tr><tr><td>Consitt et al. [76]</td><td>2016</td><td>●</td><td>●</td><td>○</td><td>●</td><td>○</td><td>○</td><td>●</td><td>●</td><td>○</td><td>●</td><td>●</td><td>7</td></tr><tr><td>DiPietro et al. [71]</td><td>2008</td><td>●</td><td>●</td><td>○</td><td>●</td><td>○</td><td>○</td><td>●</td><td>●</td><td>○</td><td>●</td><td>●</td><td>7</td></tr><tr><td>Kim et al. [56]</td><td>2017</td><td>●</td><td>●</td><td>○</td><td>●</td><td>○</td><td>○</td><td>●</td><td>●</td><td>○</td><td>●</td><td>●</td><td>7</td></tr><tr><td>Krishnan et al. [65]</td><td>2014</td><td>●</td><td>●</td><td>○</td><td>●</td><td>○</td><td>○</td><td>●</td><td>●</td><td>○</td><td>●</td><td>●</td><td>7</td></tr><tr><td>Praksch et al. [78]</td><td>2019</td><td>●</td><td>●</td><td>○</td><td>●</td><td>○</td><td>○</td><td>●</td><td>●</td><td>○</td><td>●</td><td>●</td><td>7</td></tr><tr><td>Yamada et al. [79]</td><td>2015</td><td>●</td><td>●</td><td>○</td><td>●</td><td>○</td><td>○</td><td>●</td><td>●</td><td>○</td><td>●</td><td>●</td><td>7</td></tr><tr><td>Im et al. [10]</td><td>2019</td><td>●</td><td>●</td><td>○</td><td>●</td><td>○</td><td>○</td><td>●</td><td>●</td><td>○</td><td>●</td><td>●</td><td>7</td></tr><tr><td><strong>High-intensity interval training</strong></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>Friedenreich et al. [69]</td><td>2019</td><td>●</td><td>●</td><td>○</td><td>●</td><td>○</td><td>○</td><td>●</td><td>●</td><td>●</td><td>●</td><td>●</td><td>8</td></tr><tr><td>Vaczi et al. [59]</td><td>2014</td><td>●</td><td>●</td><td>○</td><td>●</td><td>○</td><td>○</td><td>●</td><td>●</td><td>○</td><td>●</td><td>●</td><td>7</td></tr><tr><td>Søgaard et al. [77]</td><td>2019</td><td>●</td><td>○</td><td>○</td><td>●</td><td>○</td><td>○</td><td>●</td><td>●</td><td>○</td><td>●</td><td>●</td><td>6</td></tr><tr><td>Hayes et al. [63]</td><td>2015</td><td>●</td><td>●</td><td>○</td><td>●</td><td>○</td><td>○</td><td>●</td><td>●</td><td>○</td><td>●</td><td>●</td><td>7</td></tr><tr><td>Hayes et al. [90]</td><td>2017</td><td>●</td><td>○</td><td>○</td><td>●</td><td>○</td><td>○</td><td>●</td><td>●</td><td>○</td><td>●</td><td>●</td><td>6</td></tr><tr><td>Micielska et al. [73]</td><td>2019</td><td>●</td><td>○</td><td>○</td><td>●</td><td>○</td><td>○</td><td>●</td><td>●</td><td>○</td><td>●</td><td>●</td><td>6</td></tr><tr><td>Motiani et al. [55]</td><td>2017</td><td>●</td><td>●</td><td>○</td><td>●</td><td>○</td><td>○</td><td>●</td><td>●</td><td>○</td><td>●</td><td>●</td><td>7</td></tr><tr><td>Ramos et al. [75]</td><td>2016</td><td>●</td><td>●</td><td>○</td><td>●</td><td>○</td><td>○</td><td>●</td><td>●</td><td>○</td><td>●</td><td>●</td><td>7</td></tr><tr><td>Sellami et al. [15]</td><td>2016</td><td>●</td><td>●</td><td>○</td><td>●</td><td>○</td><td>○</td><td>●</td><td>●</td><td>○</td><td>●</td><td>●</td><td>7</td></tr><tr><td><strong>Resistance training</strong></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>Ahtiainen et al. [60]</td><td>2011</td><td>●</td><td>●</td><td>○</td><td>●</td><td>○</td><td>○</td><td>●</td><td>●</td><td>○</td><td>●</td><td>●</td><td>7</td></tr><tr><td>Ahtiainen et al. [61]</td><td>2015</td><td>●</td><td>●</td><td>○</td><td>●</td><td>○</td><td>○</td><td>●</td><td>●</td><td>○</td><td>●</td><td>●</td><td>7</td></tr><tr><td>Banitalebi et al. [70]</td><td>2018</td><td>●</td><td>●</td><td>●</td><td>●</td><td>●</td><td>●</td><td>●</td><td>●</td><td>○</td><td>●</td><td>●</td><td>9</td></tr><tr><td>Bermon et al. [80]</td><td>1999</td><td>●</td><td>○</td><td>○</td><td>●</td><td>○</td><td>○</td><td>●</td><td>●</td><td>○</td><td>●</td><td>●</td><td>6</td></tr><tr><td>Craig et al. [58]</td><td>1989</td><td>●</td><td>○</td><td>○</td><td>●</td><td>○</td><td>○</td><td>●</td><td>●</td><td>○</td><td>●</td><td>●</td><td>6</td></tr><tr><td>Glintborg et al. [62]</td><td>2013</td><td>●</td><td>●</td><td>●</td><td>●</td><td>●</td><td>●</td><td>●</td><td>●</td><td>○</td><td>●</td><td>●</td><td>10</td></tr><tr><td>Ha et al. [72]</td><td>2018</td><td>●</td><td>●</td><td>○</td><td>●</td><td>○</td><td>○</td><td>●</td><td>●</td><td>○</td><td>●</td><td>●</td><td>7</td></tr><tr><td>Nunes et al. [66]</td><td>2019</td><td>●</td><td>●</td><td>○</td><td>●</td><td>○</td><td>○</td><td>●</td><td>●</td><td>○</td><td>●</td><td>●</td><td>7</td></tr><tr><td>Ogawa et al. [74]</td><td>2010</td><td>●</td><td>○</td><td>○</td><td>●</td><td>○</td><td>○</td><td>●</td><td>●</td><td>○</td><td>●</td><td>●</td><td>6</td></tr><tr><td>Sato et al. [67]</td><td>2014</td><td>●</td><td>○</td><td>○</td><td>●</td><td>○</td><td>○</td><td>●</td><td>●</td><td>○</td><td>●</td><td>●</td><td>6</td></tr><tr><td>Sellami et al. [16]</td><td>2018</td><td>●</td><td>●</td><td>○</td><td>●</td><td>○</td><td>○</td><td>●</td><td>●</td><td>○</td><td>●</td><td>●</td><td>7</td></tr><tr><td>Walker et al. [68]</td><td>2015</td><td>●</td><td>●</td><td>○</td><td>●</td><td>○</td><td>○</td><td>●</td><td>●</td><td>○</td><td>●</td><td>●</td><td>7</td></tr></tbody></table>
<table><caption>**Table 4** Effects of training on total testosterone concentrations in elderly people</caption><thead><tr><th rowspan="2">Reference(s)</th><th rowspan="2">Year</th><th rowspan="2">Intervention</th><th colspan="2">Population</th><th rowspan="2">Outcomes</th><th rowspan="2">Effect size</th></tr><tr><th>Age</th><th>Sex</th></tr></thead><tbody><tr><td>Ahtiainen et al. [60]</td><td>2011</td><td>Heavy resistance exercise</td><td>61 ± 5</td><td>Male</td><td>Testosterone ↑</td><td>0.38</td></tr><tr><td>Ahtiainen et al. [61]</td><td>2015</td><td>Heavy resistance exercise</td><td>70 ± 2</td><td>Male</td><td>Testosterone ↑</td><td>1.99</td></tr><tr><td>Craig et al. [58]</td><td>1989</td><td>Progressive resistance training</td><td>62.8 ± 0.7</td><td>Male</td><td>Testosterone ↑</td><td>0.40</td></tr><tr><td>Glintborg et al. [62]</td><td>2013</td><td>Strength training</td><td>68–78</td><td>Male</td><td>Testosterone ↑</td><td>0.90</td></tr><tr><td>Hayes et al. [63]</td><td>2015</td><td>Low to medium and high intensity training</td><td>61 ± 5</td><td>Male</td><td>Testosterone ↑</td><td>0.22</td></tr><tr><td>Hayes et al. [90]</td><td>2015</td><td>High-intensity interval training</td><td>62 ± 2</td><td>Male</td><td>Testosterone ↑</td><td>0.24</td></tr><tr><td>Krishnan et al. [65]</td><td>2014</td><td>Aerobic and resistance training</td><td>46.7 ± 3.3</td><td>Female</td><td>Testosterone ↑</td><td>0.19</td></tr><tr><td>Nunes et al. [66]</td><td>2019</td><td>Resistance training</td><td>64.2</td><td>Female</td><td>Testosterone ↑</td><td>0.29</td></tr><tr><td>Sato et al. [67]</td><td>2014</td><td>Resistance training</td><td>67.2 ± 1.8</td><td>Male</td><td>Testosterone ↑</td><td>3.37</td></tr><tr><td>Sellami et al. [16]</td><td>2018</td><td>Combined sprint and resistance training</td><td>40 ± 2</td><td>Male</td><td>Testosterone ↑</td><td>1.60</td></tr><tr><td>Vaczi et al. [59]</td><td>2014</td><td>Stretch shortening cycle and eccentric training</td><td>65.7 ± 5.3</td><td>Male</td><td>Testosterone ↑</td><td>0.32</td></tr><tr><td>Walker et al. [68]</td><td>2015</td><td>Resistance training</td><td>63.7 ± 3</td><td>Male</td><td>Testosterone ↑</td><td>0.39</td></tr></tbody></table>

↑ Indicates increase, ↓ indicates decrease

<table><caption>**Table 5** Effects of training on cortisol concentrations in elderly people</caption><thead><tr><th rowspan="2">Reference(s)</th><th rowspan="2">Year</th><th rowspan="2">Intervention</th><th colspan="2">Population</th><th rowspan="2">Outcomes</th><th rowspan="2">Effect size</th></tr><tr><th>Age</th><th>Sex</th></tr></thead><tbody><tr><td>Banitalebi et al. [70]</td><td>2018</td><td>Resistance and endurance training</td><td>67.3 ± 1.4</td><td>Female</td><td>Cortisol ↓</td><td>0.27</td></tr><tr><td>Friedenreich et al. [69]</td><td>2019</td><td>Moderate- and high-intensity training</td><td>59.4 ± 4.9</td><td>Female</td><td>Cortisol ↑<br>Cortisone ↑<br>Corticosterone ↑</td><td>2.69<br>0.61<br>1.12</td></tr><tr><td>Hayes et al. [63]</td><td>2015</td><td>Low- to medium- and high-intensity training</td><td>61 ± 5</td><td>Male</td><td>Cortisol ↓</td><td>0.39</td></tr><tr><td>Hayes et al. [90]</td><td>2015</td><td>High-intensity training</td><td>62 ± 2</td><td>Male</td><td>Cortisol ↓</td><td>0.46</td></tr><tr><td>Nunes et al. [66]</td><td>2019</td><td>Resistance training</td><td>64.2 ± 1.2</td><td>Female</td><td>Cortisol ↑</td><td>0.31</td></tr><tr><td>Sellami et al. [15]</td><td>2016</td><td>High-intensity sprint training and strength training</td><td>40.7 ± 1.8</td><td>Male</td><td>Cortisol ↑</td><td>0.98</td></tr><tr><td>Sellami et al. [16]</td><td>2018</td><td>Combined sprint and resistance training</td><td>40 ± 2</td><td>Male</td><td>Cortisol ↑</td><td>0.27</td></tr><tr><td>Vaczi et al. [59]</td><td>2014</td><td>Stretch shortening cycle and eccentric training</td><td>65.7 ± 5.3</td><td>Male</td><td>Cortisol ↑</td><td>0.37</td></tr><tr><td>Walker et al. [68]</td><td>2015</td><td>Resistance training</td><td>63.7 ± 3</td><td>Male</td><td>Cortisol ↑</td><td>0.38</td></tr></tbody></table>

↑ Indicates increase, ↓ indicates decrease

testosterone [59–61, 63]. Yet, benefits of exercise training in both sexes stretch beyond steroidogenesis, and include effects such as muscle mass regeneration, weight loss, disability prevention and prevention of sarcopenia [64, 65]. Differences in exercise training, such as type, intensity, frequency and duration, can potentially affect testosterone levels and muscle mass, with exercise volume being a critical component [84]. Results from this review demonstrate different forms of exercise (such as resistance training, HIIT, aerobic training) aid in maintenance of blood testosterone levels and muscle mass of the elderly. Levels of free testosterone, representing the fraction available for tissue uptake, are increased by exercise when total testosterone levels increase with no changes in SHBG levels [16]. There is much interest in regulation of testosterone levels in the aged as low testosterone is associated with many

non-communicable diseases such as diabetes [85], cardiovascular disease [86], Alzheimer's disease [87], dementia [88], obesity [89] and ultimately mortality [86]. Evidence of increases in circulating testosterone (particularly the free fraction) by non-pharmacological means (e.g., exercise) has important implications for patients and clinicians [66, 67, 90]. Nevertheless, it remains unclear if increases in blood testosterone through exercise: (a) exceed inherent analytical and biological variability [91], and (b) exert benefits on aging physiology in addition to the other effects of exercise.

## 4.2 Effects of Exercise on Cortisol

The results of our analysis indicate that effect sizes have qualitative differences [i.e., the direction of the effect (increase or decrease)] in the various studies, ranging from
<table><caption>**Table 6** Effects of training on insulin concentrations in elderly people</caption><thead><tr><th rowspan="2">Reference(s)</th><th rowspan="2">Year</th><th rowspan="2">Intervention</th><th colspan="2">Population</th><th rowspan="2">Outcomes</th><th rowspan="2">Effect size</th></tr><tr><th>Age</th><th>Sex</th></tr></thead><tbody><tr><td>Banitalebi et al. [70]</td><td>2018</td><td>Resistance and endurance training</td><td>67.3±1.4</td><td>Female</td><td>Insulin ↑</td><td>0.56</td></tr><tr><td>Consitt et al. [76]</td><td>2016</td><td>Endurance and strength training</td><td>67±3.3</td><td>Male and female</td><td>Insulin ↑</td><td>0.32</td></tr><tr><td>DiPietro et al. [71]</td><td>2008</td><td>Aerobic training and strength training</td><td>77±6</td><td>Female</td><td>Insulin ↓</td><td>0.08</td></tr><tr><td>Guia et al. [94]</td><td>2019</td><td>High intensity interval training</td><td>62.3±4.1</td><td>Male</td><td>Insulin ↓</td><td>1.90</td></tr><tr><td>Ha et al. [72]</td><td>2018</td><td>Combined resistance training and aerobic training</td><td>73.0±2.8</td><td>Female</td><td>Insulin ↓</td><td>0.22</td></tr><tr><td>Krishnan et al. [65]</td><td>2014</td><td>Aerobic and resistance training</td><td>46.7±3.3</td><td>Female</td><td>Insulin ↓</td><td>0.79</td></tr><tr><td>Micielska et al. [73]</td><td>2019</td><td>High-intensity circuit training</td><td>45±13</td><td>Female</td><td>Insulin ↓</td><td>0.34</td></tr><tr><td>Motiani et al. [55]</td><td>2017</td><td>Moderate-intensity continuous training and high-intensity training</td><td>50.0±3.6</td><td>Male</td><td>Insulin ↑</td><td>0.22</td></tr><tr><td>Nunes et al. [66]</td><td>2019</td><td>Resistance training</td><td>64.2±1</td><td>Female</td><td>Insulin ↓</td><td>0.29</td></tr><tr><td>Ogawa et al. [74]</td><td>2010</td><td>Resistance training</td><td>85.0±4.5</td><td>Female</td><td>Insulin ↓</td><td>2.30</td></tr><tr><td>Ramos et al. [75]</td><td>2016</td><td>Moderate-intensity continuous training and high-intensity training</td><td>58±7</td><td>Male and female</td><td>Insulin ↓</td><td>0.04</td></tr><tr><td>Sellami et al. [15]</td><td>2016</td><td>High-intensity sprint training and strength training</td><td>40.7±1.8</td><td>Male</td><td>Insulin ↓</td><td>0.60</td></tr><tr><td>Søgaard et al. [77]</td><td>2019</td><td>High-intensity interval training</td><td>63±1</td><td>Male and female</td><td>Insulin ↑</td><td>0.32</td></tr></tbody></table>

↑ Indicates increase, ↓ indicates decrease

<table><caption>**Table 7** Effects of training on insulin-like growth factor-1 (IGF-1) concentrations in elderly people</caption><thead><tr><th rowspan="2">Reference(s)</th><th rowspan="2">Year</th><th rowspan="2">Intervention</th><th colspan="2">Population</th><th rowspan="2">Outcomes</th><th rowspan="2">Effect size</th></tr><tr><th>Age</th><th>Sex</th></tr></thead><tbody><tr><td>Banitalebi et al. [70]</td><td>2018</td><td>Resistance and endurance training</td><td>67.3±1.4</td><td>Female</td><td>IGF-1 ↑</td><td>0.27</td></tr><tr><td>Bennefoy et al. [82]</td><td>1999</td><td>Physical activity</td><td>69.7±2.2</td><td>Male and female</td><td>IGF-1 ↑</td><td>0.46</td></tr><tr><td>Bermon et al. [80]</td><td>1999</td><td>Resistance training</td><td>70.1±1.0</td><td>Male and female</td><td>IGF-1 ↑</td><td>0.97</td></tr><tr><td>Micielska et al. [73]</td><td>2019</td><td>High-intensity circuit training</td><td>45±13</td><td>Female</td><td>IGF-1 ↑</td><td>0.32</td></tr><tr><td>Nunes et al. [66]</td><td>2019</td><td>Resistance training</td><td>64.2</td><td>Female</td><td>IGF-1 ↑</td><td>0.26</td></tr><tr><td>Ogawa et al. [74]</td><td>2010</td><td>Resistance training</td><td>85.0±4.5</td><td>Female</td><td>IGF-1 ↓</td><td>1.06</td></tr><tr><td>Praksch et al. [78]</td><td>2019</td><td>Home-based walking and aerobic training</td><td>67.4±5</td><td>Female</td><td>IGF-1 ↑</td><td>0.28</td></tr><tr><td>Sato et al. [67]</td><td>2014</td><td>Resistance training</td><td>67.2±1.8</td><td>Male</td><td>IGF-1 ↑</td><td>1.03</td></tr><tr><td>Yamada et al. [79]</td><td>2015</td><td>Walking exercise and nutrition</td><td>76.3±5.9</td><td>Male and female</td><td>IGF-1 ↑</td><td>0.51</td></tr></tbody></table>

↑ Indicates increase, ↓ indicates decrease

<table><caption>**Table 8** Effects of training on sex hormone-binding globulin (SHBG) concentrations in elderly people</caption><thead><tr><th rowspan="2">Reference(s)</th><th rowspan="2">Year</th><th rowspan="2">Intervention</th><th colspan="2">Population</th><th rowspan="2">Outcomes</th><th rowspan="2">Effect size</th></tr><tr><th>Age</th><th>Sex</th></tr></thead><tbody><tr><td>Ahtiainen et al. [61]</td><td>2015</td><td>Heavy resistance exercise</td><td>70±2</td><td>Male</td><td>SHBG ↑</td><td>0.25</td></tr><tr><td>Glintborg et al. [62]</td><td>2013</td><td>Strength training</td><td>68</td><td>Male</td><td>SHBG ↑</td><td>0.32</td></tr><tr><td>Hayes et al. [63]</td><td>2015</td><td>Low- to medium- and high-intensity training</td><td>61±5</td><td>Male</td><td>SHBG ↑</td><td>0.38</td></tr><tr><td>Hayes et al. [90]</td><td>2017</td><td>High-intensity training</td><td>62±2</td><td>Male</td><td>SHBG ↑</td><td>0.43</td></tr><tr><td>Kim et al. [56]</td><td>2017</td><td>Moderate physical activity</td><td>51</td><td>Male and female</td><td>SHBG ↑</td><td>0.32</td></tr><tr><td>Sellami et al. [16]</td><td>2018</td><td>Combined sprint and resistance training</td><td>40±2</td><td>Male</td><td>SHBG ↑</td><td>1.68</td></tr></tbody></table>

↑ Indicates increase, ↓ indicates decrease
<table><caption>**Table 9** Effects of training on human growth hormone (hGH) concentrations in elderly people</caption><thead><tr><th rowspan="2">Reference(s)</th><th rowspan="2">Intervention</th><th colspan="2">Population</th><th rowspan="2">Outcomes</th><th rowspan="2">Effect size</th></tr><tr><th>Age</th><th>Sex</th></tr></thead><tbody><tr><td>Banitaleb et al. [70]</td><td>Resistance and endurance training</td><td>67.3±1.4</td><td>Female</td><td>GH ↑</td><td>2.58</td></tr><tr><td>Craig et al. [58]</td><td>Progressive resistance training</td><td>62.8±0.7</td><td>Male</td><td>GH ↑</td><td>0.34</td></tr><tr><td>Im et al. [10]</td><td>Yoga and Korean dance</td><td>69.3±2.9</td><td>Female</td><td>GH ↑</td><td>0.74</td></tr><tr><td>Walker et al. [68]</td><td>Resistance training</td><td>63.7±3</td><td>Male</td><td>GH ↑</td><td>0.29</td></tr></tbody></table>

↑ Indicates increase, ↓ indicates decrease

<table><caption>**Table 10** Effects of training on dehydroepiandrosterone (DHEA) and dehydroepiandrosterone sulfate (DHEA-S) concentrations in elderly people</caption><thead><tr><th rowspan="2">Reference(s)</th><th rowspan="2">Intervention</th><th colspan="2">Population</th><th rowspan="2">Outcomes</th><th rowspan="2">Effect size</th></tr><tr><th>Age</th><th>Sex</th></tr></thead><tbody><tr><td>Boxer et al. [83]</td><td>DHEA supplements, aerobics and yoga</td><td>76.4±6.2</td><td>Female</td><td>DHEA-S ↑</td><td>1.37</td></tr><tr><td>Im et al. [10]</td><td>Yoga and Korean dance</td><td>69.3±2.9</td><td>Female</td><td>DHEA-S ↑</td><td>0.98</td></tr><tr><td>Kim et al. [56]</td><td>Moderate physical activity</td><td>51</td><td>Male and female</td><td>DHEA-S ↑</td><td>0.37</td></tr><tr><td>Krishnan et al. [65]</td><td>Aerobic and resistance training</td><td>46.7±3.3</td><td>Female</td><td>DHEA-S ↑</td><td>0.41</td></tr><tr><td>Nunes et al. [66]</td><td>Resistance training</td><td>64</td><td>Female</td><td>DHEA-S ↓</td><td>0.28</td></tr><tr><td>Sato et al. [67]</td><td>Resistance training</td><td>67.2±1.8</td><td>Male</td><td>DHEA-S ↑</td><td>1.71</td></tr><tr><td>Yamada et al. [79]</td><td>Walking exercise and nutrition</td><td>76.3±5.9</td><td>Male and female</td><td>DHEA-S ↑</td><td>0.55</td></tr></tbody></table>

↑ Indicates increase, ↓ indicates decrease

<figure><img src="image_3.png" /><figcaption>**Fig. 2** Top: Summary of the main effects of exercise on hormones discussed in this review. Bottom: Summary of the main effects of ageing on hormones discussed in this review. IGF-1 insulin-like</figcaption></figure>

growth factor-1, hGH human growth hormone, DHEA-S dehydroepiandrosterone sulfate, SHBG sex hormone-binding globulin. ↗ indicates increase; ↘ indicates decrease
a small decrease in cortisol to a very large increase, indicat-
ing an inconsistency in findings. Cortisol, corticosterone,
cortisone and 11-deoxycortisol are key biomarkers of stress,
particularly of acute stress [68]. Exercise training reduces
stress in the elderly and often decreases basal cortisol and
raises testosterone levels [58]. Recent studies report that
post-training basal blood cortisol levels are decreased rela-
tive to non-exercise populations, and that exercise-induced
changes in cortisol are unrelated to the volume, type and
duration of exercise [68, 69]. A study by Hayes et al. [63]
reported no improvements in blood cortisol levels after
12 weeks of training (of which 6 weeks was HIIT), although
other reports indicated that increases in blood cortisol levels
are not related to the type of exercise. It is possible that a
single exercise bout may not be sufficient to cause persistent
changes in adrenal function; however, benefits are likely if
the exercise intensity is sufficient (~60% maximal oxygen
uptake), which is common in younger adults [15]. Another
possibility is that age-related changes in the hypothalamus-
pituitary axis may alter responses to exercise [14].

4.3 Effect of Exercise on DHEA and DHEA-S

Small to very large increases of DHEA and DHEA-S post-
training were generally observed in our analysis, although
the number of studies was relatively small. Men's DHEA-S
responses were also greater than those of women [55].
DHEA levels decreased around 65 years of age and were
associated with reduced muscle strength in males but not in
females [81]. However, a significant decline in the circulat-
ing androgen DHEA-S between the ages 20 and 50 years was
associated with the normal ageing process for women [64].
We observed that exercise tends to maintain blood levels
of DHEA in both men and women. Even a single bout of
exercise has demonstrated immediate increases in circulat-
ing androgen levels (testosterone and DHEA-S) [64]. DHEA
hormonal levels are in general positively correlated with
normal physical activity, involvement in sport and aerobic
ability [93]. The effects of exercise training on DHEA-S
levels differ in males and females [55]. Several studies have
shown that serum DHEA levels in older adults are increased
significantly by exercise training [66, 78]. Recent evidence
suggests that increased levels of DHEA-S during the meno-
pausal transition can double cardiovascular disease risk and
diabetes mellitus [10].

4.4 Effect of Exercise on Insulin

We found that exercise training reduces blood insulin levels
in older adults in most studies, with four studies showing
small to moderate increases [55, 70, 76, 77]. Effect sizes
ranged from small to very large, with some variability in the
findings. These divergent effects did not seem to be related

to study duration, as those studies with the largest positive
effects [73, 91] were only 12 weeks in duration. This cor-
roborates a recent study [94] in which no changes were noted
to insulin levels measured after 12 weeks of training in life-
long exercisers or lifelong sedentary older adults. Small to
moderate effects were observed in the previously sedentary
group only, suggesting that participant selection may have
contributed to the variability we found. Insulin sensitivity
decreases with age and can lead to insulin resistance and
type 2 diabetes mellitus [70, 75]. Skeletal muscle dysfunc-
tion and disuse associated with ageing is a primary cause
of impaired glucose absorption and reduced mitochondrial
oxidation [75]. Skeletal muscles are responsible for > 60%
of glucose metabolism [72], and thus exercise in older adults
is an important regulator of glucose metabaolism. HIIT
improves insulin sensitivity, body composition and cardio-
vascular health [76], and several studies suggest the increase
in insulin sensitivity due to exercise training is directly pro-
portional to exercise intensity and volume [70, 71], although
there is also evidence that combined training may be more
efficacious than one form of training alone [64, 69–71].

4.5 Effect of Exercise on IGF-1

Levels of IGF-1 were ubiquitously increased in the all the
studies we analysed, with small to large effect sizes being
evident. IGF-1 plays an important role in muscle develop-
ment and insulin sensitivity [65], and changes in IGF-1 are
correlated with cardiovascular disease risk and mortality
[77]. IGF-1 levels decrease during ageing (i.e., the somato-
pause), but several exercise types can affect the synthesis of
IGF-1 [65, 66, 72, 77]. Increased IGF-1 levels cause down-
stream improvements in insulin activity [65, 66, 77]. Resist-
ance training reportedly causes greater increases in IGF-1
relative to endurance training [65, 69]. Combined training
with different exercise sequences did not affect IGF-1 [69].
When comparing concurrent training with interval training,
concurrent training produced greater increases in IGF-1 lev-
els [69]. Yet, high-intensity circuit training also produced
significant elevations of IGF-1 that were accompanied by
improvements in the homeostatic model assessment index
of insulin resistance (HOMA1-IR) [72]. One important issue
to consider from these data is the interpretation of increased
IGF-1 levels. For example, Herbert et al. [33] reported
increased IGF-1 following HIIT in previously sedentary
older masters athletes, who had higher levels of IGF-1 than
sedentary individuals, causing these authors to propose
increased IGF-1 in older men was a positive physiological
adaptation. Conversely, Hayes et al. [95] concluded IGF-1
decreases were a positive physiological adaptation following
resistance training. These authors hypothesized that IGF-1
entered muscle tissue to exert downstream hypertrophic
effects during periods of muscle building (i.e., anabolism).
Muscle hypertrophy is not the only response to perturbations in IGF-1, with other effects of IGF-1 possible in participants who exercise.

## 4.6 Effect of Exercise on SHBG

Our analysis indicates that levels of SHBG in older adults were universally increased by exercise. This glycoprotein binds to androgens and oestrogens and its levels increase with age whilst testosterone decreases, resulting in lower levels of bioavailable and free testosterone available for biological effects following tissue uptake. Therefore, it is imperative to measure both SHBG and testosterone levels to obtain a better understanding of androgenic status. In this context, Hayes et al. [63] previously reported increases in total testosterone and SHBG levels, with no alterations in free testosterone levels [62]. There were larger increases in total testosterone SHBG when HIIT was used as the exercise protocol, suggesting increases in free testosterone [63]. Therefore, it is important to distinguish which hormone fraction is being measured in such studies. Low SHBG suggests a high androgenic nature in women, which is inversely related with adiposity and SHBG levels [55].

## 4.7 Effect of Exercise on Human Growth Hormone

We found only four studies that measured hGH, all which reported increases in hGH having effect sizes from small to very large [10, 58, 68, 70]. One possible reason for the limited number of studies measuring hGH may be its short biological half-life, making it more pragmatic to measure IGF-1, the main downstream protein of hGH secretion [68]. When comparing other forms of exercise, resistance training causes the largest increases in hGH levels in older adults [67]. Ageing accelerates the reduction of hGH secretion in both sexes, with females experiencing steeper declines than males [67, 69, 77]. Combined exercise programs such as resistance and endurance increase hGH levels [69].

## 5 Conclusion

In conclusion, exercise increases the levels of anabolic hormones in older adults, although the clinical significance of these alterations remains uncertain. It is apparent that exercise exerts anti-ageing effects on several physiological systems, but whether these effects are mediated by the endocrine system is unclear at this time. Nonetheless, we recommend that exercise should be considered as a first-line treatment for endocrine dysfunction as it improves several changes of the hormonal regulation that occur with ageing. Future investigations may wish to address the effects of exercise on hormonal concentrations in middle-aged

individuals as this is typically where age-associated hormonal milieu alterations may begin to manifest. In the present review, regrettably we found that too few original articles were conducted in participants aged 40–60 years, so additional articles in this age category would have allowed us to examine age differences and would have permitted practical applications for each age category.

**Data Availability Statement** All data supporting the findings of this study are available in this published article.

### Declarations

**Funding** No sources of funding were used to assist in the preparation of this article.

**Conflicts of Interest** Hassane Zouhal, Ayyappan Jayavel, Kamalanathan Parasuraman, Lawrence D Hayes, Claire Tourny, Fatma Rhibi, Ismail Laher, Abderraouf Ben Abderrahman and Anthony C. Hackney declare that they have no conflicts of interest relevant to the content of this review.

**Author Contributions** Hassane Zouhal was involved in the conceptualization of the study, data analysis and writing the manuscript. Ayyappan Jayavel, Kamalanathan Parasuraman, Lawrence D. Hayes, Claire Tourny, Fatma Rhibi, Ismail Laher, Abderraouf Ben Abderrahman and Anthony C. Hackney were involved in the data assessment, data analysis and writing the manuscript. All authors approved the final version of the manuscript.

**Open Access** This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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## Authors and Affiliations

Hassane Zouhal¹,² ID • Ayyappan Jayavel³ • Kamalanathan Parasuraman³ • Lawrence D. Hayes⁴ • Claire Tourny⁵ • Fatma Rhibi¹ • Ismail Laher⁶ • Abderraouf Ben Abderrahman⁷ • Anthony C. Hackney⁸

☑ Hassane Zouhal
hassane.zouhal@univ-rennes2.fr

☑ Anthony C. Hackney
ach@email.unc.edu

¹ M2S, Laboratoire Mouvement, Sport, Santé, EA 1274, Université Rennes, 35000 Rennes, France

² Institut International des Sciences du Sport (212S), 35850 Irodouer, France

³ SRM College of Physiotherapy, SRM Institute of Science and Technology, SRM Nagar, Kattankulathur, Kanchipuram, Chennai, TN 603203, India

⁴ Institute of Clinical Exercise and Health Science, School of Health and Life Sciences, University of the West of Scotland, Lanarkshire Campus, Glasgow G72 0LH, UK

⁵ EA 3832 CETAPS, University of Rouen, Rouen, France

⁶ Faculty of Medicine, Department of Anesthesiology, Pharmacology and Therapeutics, The University of British Columbia, Vancouver, Canada

⁷ ISSEP Ksar-Said, University of La Manouba, Tunis, Tunisia

⁸ Department of Exercise and Sport Science, Department of Nutrition, University of North Carolina, Chapel Hill, NC, USA