# Transient overconsumption induced by a Western diet in rats is essential to the control of metabolic endotoxemia via regulation of LPS intestinal translocation

Mathilde Guerville, Annaëlle Sinquin, Fabienne Laugerette, Marie-Caroline

Michalski, Gaëlle Boudry

► To cite this version:

Mathilde Guerville, Annaëlle Sinquin, Fabienne Laugerette, Marie-Caroline Michalski, Gaëlle Boudry. Transient overconsumption induced by a Western diet in rats is essential to the control of metabolic endotoxemia via regulation of LPS intestinal translocation. 13. International congress on obesity, May 2016, Vancouver, Canada. Wiley Online Library, Obesity Reviews, 17 (Suppl. 2), 2016, Obesity Reviews. hal-01594521

HAL Id: hal-01594521

https://hal.science/hal-01594521

Submitted on 3 Jun 2020

HAL is a multi-disciplinary open access
archive for the deposit and dissemination of sci-
entific research documents, whether they are pub-
lished or not. The documents may come from
teaching and research institutions in France or
abroad, or from public or private research centers.

L'archive ouverte pluridisciplinaire HAL, est
destinée au dépôt et à la diffusion de documents
scientifiques de niveau recherche, publiés ou non,
émanant des établissements d'enseignement et de
recherche français ou étrangers, des laboratoires
publics ou privés.

<figure><img src="image_1.png" /></figure>
T2:S14:02

Transient overconsumption induced by a western
diet in rats is essential to the control of metabolic
endotoxemia via regulation of LPS intestinal
translocation

Guerville, M.¹*, Sinquin, A.¹, Laugerette, F.²,
Michalski, M.C.² and Boudry, G.¹

¹UR1341 ADNC, INRA Saint-Gilles; ²CarMeN, Lyon

Increased plasma level of gut bacteria-derived lipopolysaccharides (metabolic endotoxemia) due to alteration of intestinal homeostasis plays an important role in obesity-related disorders. We hypothesized that initial calorie overconsumption during Western diet feeding in rats alters intestinal homeostasis, leading to increased lipopolysaccharides (LPS) translocation.

Rats were fed for 1 or 6 weeks either a control diet (C), a Western Diet (45% fat) *ad libitum* (WDal) or at the same caloric level than C rats (WDpf).

Adiposity and weight gain were similarly increased in both groups of WD rats, whereas endotoxemia was increased only in rats fed WD without calorie overconsumption. WD feeding increased intestinal LPS detoxification mechanisms (alkaline phosphate activity) and transiently decreased caecal bile acids receptors mRNA, irrespective of the energy intake level. However, sustained increased in intestinal LPS permeability and decreased caecal pxr, md2 and myd88 mRNA level were observed in WDpf rats as opposed to transient effects at week 1 in WDal rats.

A Western diet has both profound and transient effects on the mech-
anisms preventing LPS intestinal translocation. The transient effects
seem to be driven by initial calorie intake and involved in the con-
trol of metabolic endotoxemia.

T2:S14:03

The effect of a multi-strain probiotic on metabolic
bio-markers in adults with prediabetes and recently
diagnosed type 2 diabetes mellitus (T2DM)

Palacios, T.¹*, Vitetta, L.², Coulson, S.², Butt, H.³ and Caterson, I.¹

¹The Boden Institute of Obesity, Nutrition, Exercise & Eating Disorders, The University of Sydney; ²The University of Sydney and Medlab Clinical Sydney; ³Bioscreen, Bio21, Molecular Science & Biotechnology Institute, University of Melbourne

T2DM is a metabolic condition characterised by a persistent low-
grade inflammatory response associated with the development of
insulin resistance1. Variations in the type, diversity and metabolic
capacity of gastrointestinal (GI) microbial communities have shown
to alter metabolic and inflammatory pathways within the host by
shifting energy balance and storage and promoting metabolic
endotoxaemia2-5. An evidence-based multi-strain probiotic has
been developed to restore the GI bacterial composition from a
disease-prone to a balanced state and to improve metabolic markers
associated with T2DM. The aim of this study is to assess the thera-
peutic effect of this novel probiotic on glucose metabolism in adults
diagnosed with prediabetes and early T2DM.

Sixty adults with a BMI ≥25 kg/m2 and diagnosed with pre-
diabetes or T2DM (within the previous 12 months) are being en-
rolled in a double-blind controlled clinical trial and randomised to
a multi-strain probiotic or placebo for 12 weeks. Both groups re-
ceive lifestyle advice. Outcome measures include fasting plasma glu-
cose, 2-hour glucose tolerance, insulin, lipids, inflammatory
markers, GI permeability, and faecal microbial and metabolomics

profiles. Measurements and samples are collected at baseline and
12 weeks after treatment. The initial results of this study will be
reported.

Intentional manipulation of GI microbial profiles may be useful for regulating T2DM-associated metabolic disorders.

T2:S14:04

Bifidobacterium animalis ssp. lactis 420 with or
without Litesse®Ultra controls body fat mass and
waist circumference in overweight and obesity—
randomized, double-blind, multicenter clinical study

Stenman, L.K.¹*, Lehtinen, M.J.¹, Meland, N.²,
Kloster Smerud, H.², Rissanen, A.³ and Lahtinen, S.¹

¹DuPont Nutrition & Health, Active Nutrition; ²Smerud Medical Research; ³Obesity Research Unit, Biomedicum, University of Helsinki

The composition of gut microbiota is interlinked with energy bal-
ance, but causal evidence between its modulation and body fat mass
is still very scarce. We investigated the effects of *Bifidobacterium
animalis* ssp. *lactis* 420 (B420) and a dietary fiber, Litesse®Ultra
polydextrose (LU), on body fat mass and other parameters related
to obesity.

A total of 225 healthy participants were randomized into four
groups for 6 mo of treatment: 1) Placebo; 2) LU, 12 g/d; 3) B420,
1010 CFU/d; 4) LU+B420, 12 g + 1010 CFU/d. Participants main-
tained their habitual diet and exercise routines. Body composition
(DEXA) and anthropometric measurements were taken at 0, 2, 4
and 6 months, as well as one month after end of treatment.

The Per Protocol analysis (n=134) included participants who com-
pleted the treatment without major protocol violations, as was
pre-defined. For change in body fat mass, LU+B420 showed a -
1.4 kg difference to Placebo (P=0.02), whereas LU alone had no ef-
fect, and the overall ANCOVA was non-significant (P=0.095). The
factorial analysis for total fat mass was significant for B420 (P =
0.002 vs. Placebo). The effects on body fat mass were most pro-
nounced in the abdominal region, reflected by a reduction in waist
circumference in the LU+B420 group (-2.5 cm, P = 0.047 vs. Pla-
cebo, ANCOVA P = 0.10).

B420 and LU+B420 showed benefits for controlling body fat mass and waist circumference, whereas LU had no effect. (Clinicaltrials.gov NCT01978691)

Sponsored by DuPont Nutrition & Health.

T2:S14:05

Abdominal obesity is inversely associated with small
intestinal bacterial overgrowth in irritable bowel
syndrome

Kim, K.N., Joo, N.S., Jung, S.E., Kim, S.H. and Han, K.S.*
Ajou University School of Medicine

There are several studies considering obesity and small intestinal bacterial overgrowth (SIBO) as the risk factors for irritable bowel syndrome (IBS). But the relationship between obesity and SIBO is not studied yet in IBS. The aim of this study was to investigate the association between obesity by body mass index (BMI) or waist circumference (WC) and SIBO in IBS patients.

We reviewed the charts of patients who showed IBS symptoms with documented results of lactulose breath test (LBT) for SIBO. Univariate and multivariate models were used to assess the association between obesity and SIBO. Obesity was defined as a BMI equal to or above 25. Abdominal obesity was defined as a WC equal to or above 85 cm in women and 90 cm in men.