# EORTC SPECTA-AYA: A unique molecular profiling platform for adolescents and young adults with cancer in Europe

Teresa de Rojas, Bernd Kasper, Winette van der Graaf, Stefan M Pfister, Franck Bielle, Teresa Ribalta, Patrick Shenjere, Matthias Preusser, Stefan Fröhling, Vassilis Golfinopoulos, et al.

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Teresa de Rojas, Bernd Kasper, Winette van der Graaf, Stefan M Pfister, Franck Bielle, et al. EORTC SPECTA-AYA: A unique molecular profiling platform for adolescents and young adults with cancer in Europe. International Journal of Cancer, 2019, 147, pp.1180 - 1184. 10.1002/ijc.32651. hal-04512748

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Short Report

EORTC SPECTA-AYA: A unique molecular profiling platform
for adolescents and young adults with cancer in Europe

Teresa de Rojas<sup>1</sup>, Bernd Kasper<sup>2</sup>, Winette Van der Graaf<sup>3,4</sup>, Stefan M. Pfister<sup>5</sup>, Franck Bielle<sup>6</sup>, Teresa Ribalta<sup>7</sup>, Patrick Shenjere<sup>8</sup>, Matthias Preusser<sup>9</sup>, Stefan Fröhling<sup>10</sup>, Vassilis Golfinopoulos<sup>1</sup>, Marie Morfouace<sup>1*</sup> and Martin G. McCabe<sup>1**</sup>

For most adolescent and young adult (AYA) cancers, age-specific molecular features are poorly understood. EORTC-SPECTA, an academic translational research infrastructure for biomaterial collection, will explicitly recruit AYA patients and will therefore collect empirical data to bridge the molecular gap between pediatric and adult oncology. The initial pilot study, activated in February 2019 across Europe, will recruit 100 AYA patients (aged 12–29 years) with newly diagnosed or relapsed high-grade gliomas and high-grade bone and soft tissue sarcomas. The primary objective of the pilot is to determine feasibility and recruitment rates. Formalin-fixed tumor tissue and whole blood from study participants will be prospectively collected with clinical data and stored centrally at the Integrated BioBank of Luxembourg. Whole exome sequencing of matched tumor and blood, and tumor RNA sequencing and DNA methylation profiling will be performed at the German Cancer Research Center, Heidelberg, Germany. Virtual central pathology review of scanned diagnostic slides will be undertaken by an international

<footnote n="1"><sup>1</sup>EORTC Headquarters, Brussels, Belgium</footnote>

<footnote n="2"><sup>2</sup>Sarcoma Unit, Mannheim University Medical Center, University of Heidelberg, Mannheim, Germany</footnote>

<footnote n="3"><sup>3</sup>Netherlands Cancer Institute van Leeuwenhoek, Amsterdam, The Netherlands</footnote>

<footnote n="4"><sup>4</sup>Department of Medical Oncology, Radboud University Medical Center, Nijmegen, The Netherlands</footnote>

<footnote n="5"><sup>5</sup>Hopp Children's Cancer Center Heidelberg (KiTZ), Division of Pediatric Neurooncology, German Cancer Consortium (DKTK), German Cancer Research Center (DKFZ) and Department of Pediatric Hematology and Oncology, Heidelberg University Hospital, Heidelberg, Germany</footnote>

<footnote n="6"><sup>6</sup>Assistance Publique-Hôpitaux de Paris, La Pitié-Salpêtrière Hospital, Department of Neuropathology, Paris, France</footnote>

<footnote n="7"><sup>7</sup>Department of Pathology, Hospital Sant Joan de Deu, Barcelona, Spain</footnote>

<footnote n="8"><sup>8</sup>Department of Histopathology, The Christie NHS Foundation Trust, Manchester, United Kingdom</footnote>

<footnote n="9"><sup>9</sup>Division of Oncology, Department of Medicine I, Medical University of Vienna, Vienna, Austria</footnote>

<footnote n="10"><sup>10</sup>Department of Translational Medical Oncology, National Center for Tumor Diseases (NCT) Heidelberg, German Cancer Research Center (DKFZ), Heidelberg, Germany</footnote>

<footnote n="11"><sup>11</sup>Division of Cancer Sciences, Manchester Academic Health Science Centre, University of Manchester, Manchester, United Kingdom</footnote>

<footnote>*M.M. and M.G.M. are co-senior authors.</footnote>

<footnote>**Key words:** adolescents and young adults, molecular profiling, personalized medicine, high grade glioma, sarcoma</footnote>

<footnote>**Conflict of Interest:** M.P. has received honoraria for lectures, consultation or advisory board participation from the following for-profit companies: Bayer, Bristol-Myers Squibb, Novartis, Gerson Lehrman Group (GLG), CMC Contrast, GlaxoSmithKline, Mundipharma, Roche, Astra Zeneca, AbbVie, Lilly, Medahead, Daiichi Sankyo, Merck Sharp & Dome. F.B. has an immediate family member employed, owns stocks or received research funding from the following for-profit companies: Celgene, Crossject, Sanofi, Abbvie. S.F. has had a consulting or advisory role, received honoraria, research funding and/or travel/accommodation expenses funding from the following for-profit companies: Bayer, Roche, Amgen, Eli Lilly, PharmaMar, AstraZeneca and Pfizer. W.V.D.G. has received research funding from Novartis and advisory board honoraria from Bayer. The rest of the authors have no conflict of interest to disclose.</footnote>

<footnote>**Grant sponsor:** Fonds Cancer (FOCA); **Grant sponsor:** Unai Kest Memorial Run</footnote>

<footnote>This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.</footnote>

<footnote>**DOI:** 10.1002/ijc.32651</footnote>

<footnote>**History:** Received 27 May 2019; Accepted 12 Aug 2019; Online 29 Aug 2019</footnote>

<footnote>**Correspondence to:** Teresa de Rojas, EORTC Headquarters, Av. E. Mounier 83/11 – 1200 Brussels, Belgium, Tel.: +32-27741560, E-mail: teresa.derojas@eortc.org</footnote>
population of young patients who develop cancers typically
found in older adult populations.

The initial pilot study, activated in February 2019, will be composed of 100 AYA patients with high-grade gliomas and high-grade bone and soft tissue sarcomas, the two cancer groups responsible for most deaths in this age group. The primary objective of the pilot is to determine feasibility and recruitment rates of eligible AYA cancer patients who consent to genetic analysis of their tumor and constitutional DNA.

## Methods: Study Design and Workflow

SPECTA is a collaborative, pan-European translational research infrastructure that enables biomaterial collection for patients treated in clinical trials or per standard of care. In SPECTA-AYA, patients aged between 12 and 29 years with newly diagnosed, or any relapsed high-grade glioma, including cases with malignant progression from lower-grade tumors, and nongrade 1 bone or soft tissue sarcoma will be recruited. Formalin-fixed tumor tissue and whole blood from study participants will be prospectively collected with clinical data.

The SPECTA-AYA project is being carried out in accordance with The Code of Ethics of the World Medical Association (Declaration of Helsinki). All procedures are and will be performed in compliance with the laws and regulations of each participating country, and the appropriate institutional ethics committee(s) have approved them as required by the applicable national legislation. Informed consent and assent is being obtained for all patients or legal representatives according to the age and to the legal requirements of each country. Particular attention is given in the information sheets and informed consent forms to the implications of genetic testing, especially in regard to profiling of the germline and to the management of incidental findings.

Funds from the global EORTC-SPECTA were allocated to this initial pilot project. As for the expanded cohorts foreseen to be started in SPECTA-AYA after this pilot, we believe the prospect of securing funding to be fairly good, given the growing interest by multi-stakeholder platforms to invest in precision medicine initiatives, especially for rare cancers.

The study workflow is shown in Figure 1. The biological samples will be stored centrally and nucleic acids extracted at the Integrated BioBank of Luxembourg (IBBL). Whole exome sequencing (WES) of matched tumor and blood, and tumor RNA sequencing and DNA methylation profiling (EIPC array, Illumina) will be performed at the German Cancer Research Center (DKFZ), Heidelberg. The genomic data will be deposited on the European Genome-Phenome Archive.¹⁴ Virtual central pathology review of scanned diagnostic slides will be undertaken by an international expert panel, and diagnostic material returned to the participating centers. A multidisciplinary molecular tumor board, held monthly, will release a clinically validated report that we aim to be available to referring clinicians within 4 to 6 weeks after biopsy. Leftover material from the

collected formalin-fixed tumor tissue and blood will be stored for future research.

## Discussion: Innovative Aspects

SPECTA-AYA is an ambitious, European collaborative project that includes several innovative aspects, which ought to be highlighted.

While there are several ongoing molecular profiling initiatives across Europe for children and adults with cancer, SPECTA-AYA is the first comprehensive, international platform specifically targeted to AYA patients. AYAs with cancer constitute a unique age group with distinct features regarding epidemiology, tumor biology, inclusion rates into clinical trials, clinical outcomes and psychosocial setting.⁵,¹⁵⁻¹⁷ SPECTA-AYA will help decipher the tumor peculiarities of this group of patients and aims to improve their access to novel drugs and personalized medicine.

Scarcity of available tumor material and a preponderance of formalin-fixed over frozen tissue are recognized to be major challenges of translational research¹⁸ in rare populations such as this study population. Collection and storage of frozen tissue is increasing with time for childhood cancers and is viewed as standard of care in some countries, but it is not the norm for AYA patients in general or for the histologies included in SPECTA-AYA specifically. To understand better the biology of AYA cancers, in this study, we aim to utilize nucleic acids from blood and from the paraffin-embedded tissue almost universally available from diagnostic biopsies to generate a complete molecular profile for each patient. RNA sequencing, where quality permits, will be used to identify gene fusions and gene expression, WES will identify numerical and sequence variants, tumor mutation burden and microsatellite instability calculation and, finally, methylation array will be used to validate the tumor classification¹⁹⁻²¹ and inform copy number variations. Germline DNA will be used as control for WES and to assess predisposition syndromes, as described in Reference 22. Finally, circulating tumor DNA will also be extracted from blood samples and stored for future research.

Another hurdle in this rare patient population is the need for central pathology review. In SPECTA-AYA, a virtual pathology review platform has been developed. After patient registration, diagnostic slides (H&E and selected stainings) will be sent to IBBL, scanned at 40× magnification and uploaded onto the telpathology platform AGOKO²³ with associated clinical data. Immunostained slides will be returned to the recruiting site. An international panel of expert pathologists will review each case and make a consensus pathological diagnosis according to an agreed minimal histopathological and immunohistochemical marker set. Additional stains, if required, will be performed at a central laboratory. A comparison with the methylation classifier²⁰ will also be performed and will contribute to the agreed final diagnosis. A certain degree of overlap in the molecular analyses performed locally as part of initial diagnosis can be expected and may be instructive, as for instance, some molecular features tested by immunohistochemistry in the local pathology
<figure><img src="image_2.png" /><figcaption>Figure 1. Study workflow.</figcaption></figure>

laboratory can be corroborated by the techniques applied in SPECTA-AYA centrally, enhancing the power of the central pathology review. This can prove particularly relevant in this setting of rare tumors, in which the pathology diagnosis is frequently challenging.

The molecular tumor board will include expert pathologists, molecular biologists and clinicians, including the recruiting clinician, and will take place monthly, an approach that has been shown to be feasible in cancer networks.8,10,24 The final report released to the recruiting center will include an agreed clinical, pathological and molecular diagnosis, a rationale for molecularly targeted treatments where appropriate, and suggestions for clinical trial inclusion if available. Germline mutations, if any, and relevant incidental findings are included as well. Additionally, the clinician (on behalf of the patient and family) can request the comprehensive sequencing raw data.

The SPECTA infrastructure also allows for long-term follow-up and collection of additional clinical data including patient-reported and neurocognitive outcomes. Such variables have not been selected for the AYA pilot project, but could be easily added in a follow-up cohort.

The pan-European nature of the project, the comprehensive molecular analysis, and the virtual central pathology review process combine into a complex workflow (Fig. 1) with subsequent operational challenges. The collaboration between three major academic cancer research organizations in Europe—the UK National Cancer Research Institute, EORTC and DKFZ—has been a major facilitator in mastering these challenges. Furthermore, while decentralized storage greatly reduces the chances of prompt and reliable translational research, the involvement of IBBL as a centralized solution with shared standard operating procedures to ensure best practice in biomaterial and clinical
data collection is a key strength of SPECTA. The collaboration with DKFZ ensures that the latest genomic analysis and bioinformatic assessment will be performed during the course of the project. Finally, data and sample sharing policies are already in place at EORTC to promote academic collaboration and research, especially needed in the field of rare cancers. It is therefore EORTC's policy to consider for sharing all data generated from its research while safeguarding intellectual property, the privacy of patients and confidentiality.²⁵ This is done upon request from qualified scientific and medical researchers, for reanalysis and other research purposes. EORTC complies with the OECD Principles and Guidelines for Access to Research Data from Public Funding and with the National Institutes of Health Final Statement on Sharing Research Data released on February 26, 2003; it is committed to do so within the limits needed to guarantee the protection of personal data in accordance with the EU data protection rules and other applicable European legislation.

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## Conclusions/Output

SPECTA-AYA constitutes a major opportunity to gain knowledge about the tumor biology of this unique age group. It incorporates notable innovative aspects: AYA specificity, pan-European academic collaboration, centralized biobanking, comprehensive molecular profiling and virtual central pathology review, among others. We believe that the expected challenges can be overcome with strong collaborative efforts, notably between adult and pediatric oncology, such as the DKFZ-EORTC partnership. SPECTA-AYA will help untangle the tumor particularities of AYAs with cancer and, in the longer term, aims to improve their access to novel drugs and personalized medicine.

## Acknowledgements

This publication was partially supported by Unai Kest Memorial Run. The work of T.d.R. as Fellow at EORTC Headquarters was supported by a grant from Fonds Cancer (FOCA) from Belgium. We would like to thank Laurence Decroix and Frederic Rince, both members of the EORTC Communication Team, for producing the workflow figure.