# Adventitious regeneration separates pear chimeras into their component types and induces unstable variegation

Elisabeth Chevreau, C. Leblay, Louis-Marie Raboin

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Elisabeth Chevreau, C. Leblay, Louis-Marie Raboin. Adventitious regeneration separates pear chimeras into their component types and induces unstable variegation. VII. International Congress on Plant Tissue and Cell Culture, Jun 1990, Amsterdam, Netherlands. hal-01601034

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https://hal.science/hal-01601034v1

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Amsterdam, June 24-29, 1990

Abstracts VIth International
Congress on Plant Tissue and
Cell Culture

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# ADVENTITIOUS REGENERATION SEPARATES PEAR CHIMERAS INTO THEIR COMPONENT TYPES AND INDUCES UNSTABLE VARIEGATION

E. CHEVREAU, C. LEELAY, L.M. RABOIN

INRA Station d'Amélioration des Espèces Fruitières et Ornementales Beaucouzé 49000 Angers FRANCE

A variegated mutant of the pear variety 'Louise Bonne' has a chimeral structure G/G/A (L1 green, L2 green, L3 albino). A few albino shoots appeared during the in vitro multiplication of this plant, indicating the relative instability of this periclinal chimera. Adventitious bud regeneration has been obtained on leaf pieces. Among the regenerants from the variegated plants, more than 99% were green, and only 2 plants were albino, whereas 96% of the regenerants from the albino plants were green and 4% albino. This indicates that the albino plants still contained an epidermis of the green type, undetectable because of the absence of chloroplasts. The results suggest that most of the regenerated buds originated from the epidermal layer, and that regeneration from the L2 and L3 layers occured at very low frequencies. So far, the phenotype of all the regenerated plants is stable, demonstrating that separation of the variegated chimera into pure types has been achieved. In another experiment on the non-variegated pear variety 'Conférence', adventitious regeneration from leaf pieces gave a chlorophyll deficient variant with an unstable variegation pattern. Regeneration has been obtained from leaves of this plant. Variegated leaves produced 45% green, 45% albino and 10% variegated plants, while green leaves produced 86% green and 14% albino plants. The stability of these regenerants is now being tested. The occurrence of a transposon-like phenomenon is postulated.