# Cohort Study of Maternal Gestational Weight Gain, Gestational Diabetes, and Childhood Asthma

Orianne Dumas, Anna Chen Arroyo, Mohammad Kamal Faridi, Kaitlyn E. James, Sarah Hsu, Camille E. Powe, Carlos Arturo Camargo

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Orianne Dumas, Anna Chen Arroyo, Mohammad Kamal Faridi, Kaitlyn E. James, Sarah Hsu, et al. Cohort Study of Maternal Gestational Weight Gain, Gestational Diabetes, and Childhood Asthma. Nutrients, 2022, 14 (23), 10.3390/nu14235188. [DOI: 10.3914/nu.14235188](https://doi.org/10.3914/nu.14235188)

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Article

# Cohort Study of Maternal Gestational Weight Gain, Gestational Diabetes, and Childhood Asthma

Orianne Dumas <fnref n="1" />,* ID, Anna Chen Arroyo <fnref n="2" />, Mohammad Kamal Faridi <fnref n="3" />, Kaitlyn James <fnref n="4" />, Sarah Hsu <fnref n="5" />,<fnref n="6" />, Camille Powe <fnref n="4" />,<fnref n="5" />,<fnref n="7" />,<fnref n="8" /> and Carlos A. Camargo, Jr. <fnref n="3" /> ID

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**Citation:** Dumas, O.; Arroyo, A.C.; Faridi, M.K.; James, K.; Hsu, S.; Powe, C.; Camargo, C.A., Jr. Cohort Study of Maternal Gestational Weight Gain, Gestational Diabetes, and Childhood Asthma. *Nutrients* **2022**, *14*, 5188. https://doi.org/10.3390/nu14235188

Academic Editor: Annunziata Lapolla

Received: 3 November 2022

Accepted: 2 December 2022

Published: 6 December 2022

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**Copyright:** © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

**Abstract:** Data on the association of maternal gestational weight gain (GWG) and gestational diabetes mellitus (GDM) with childhood asthma are limited and inconsistent. We aimed to investigate these associations in a U.S. pre-birth cohort. Analyses included 16,351 mother–child pairs enrolled in the Massachusetts General Hospital Maternal-Child Cohort (1998–2010). Data were obtained by linking electronic health records for prenatal visits/delivery to determine BMI, GWG, and GDM (National Diabetes Data Group criteria) and to determine asthma incidence and allergies (atopic dermatitis or allergic rhinitis) for children. The associations of prenatal exposures with asthma were evaluated using logistic regression adjusted for maternal characteristics. A total of 2306 children (14%) developed asthma by age 5 years. Overall, no association was found between GWG and asthma. GDM was positively associated with offspring asthma (OR 1.46, 95% CI 1.14–1.88). Associations between GDM and asthma were observed only among mothers with early pregnancy BMI between 20 and 24.9 kg/m<fnref n="2" /> (OR 2.31, CI 1.46–3.65, p-interaction 0.02). We report novel findings on the impact of prenatal exposures on asthma, including increased risk among mothers with GDM, particularly those with a normal BMI. These findings support the strengthening of interventions targeted toward a healthier pregnancy, which may also be helpful for childhood asthma prevention.

**Keywords:** gestational diabetes; gestational weight gain; allergy; childhood asthma; developmental origins of health and disease

## 1. Introduction

Asthma is the most common chronic disease in children [1], with a prevalence of 10.5% in U.S. children [2]. Asthma is associated with a lifelong morbidity in many individuals [3] and generates a high social and economic burden [1]. Early life is increasingly emphasized as a crucial period in the development of chronic respiratory and allergic diseases, such as asthma [4]. Prenatal determinants of asthma are of particular interest as pregnancy is seen as a window of opportunity for primary prevention [4,5], especially considering maternal risk factors that can be partly modified through lifestyle and nutritional interventions, such as body mass index (BMI), gestational weight gain (GWG), and gestational diabetes mellitus (GDM) [6,7]. Although cohort studies have shown associations between a high maternal BMI before or during pregnancy and risk of childhood asthma [8,9], the independent roles of GWG and GDM remain uncertain [9,10].

<footnote n="1">¹ Université Paris-Saclay, UVSQ, Univ. Paris-Sud, Inserm, Équipe d'Épidémiologie Respiratoire Intégrative, CESP, 94807 Villejuif, France</footnote>

<footnote n="2">² Division of Pulmonary, Allergy & Critical Care Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA</footnote>

<footnote n="3">³ Department of Emergency Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA</footnote>

<footnote n="4">⁴ Department of Obstetrics and Gynecology, Massachusetts General Hospital, Boston, MA 02114, USA</footnote>

<footnote n="5">5 Diabetes Unit, Massachusetts General Hospital, Boston, MA 02114, USA</footnote>

<footnote n="6">⁶ Clinical and Translational Epidemiology Unit, Massachusetts General Hospital, Boston, MA 02114, USA</footnote>

<footnote n="7">7 Harvard Medical School, Boston, MA 02115, USA</footnote>

<footnote n="8">8 Broad Institute, Cambridge, MA 02142, USA</footnote>

<footnote>* Correspondence: orianne.dumas@inserm.fr</footnote>
Relatively few studies [11–15] have examined the association between GWG and child-hood asthma while simultaneously investigating maternal BMI. A recent meta-analysis reported associations with both a low GWG and a high GWG and increased risk of child-hood asthma or wheeze (U-shaped relationship) [9], but results partly differed in studies published since [16,17]. Although suggested associations are modest, the public health impact may be important, as a large proportion of women have inadequate weight gain during pregnancy [16,18,19]. Additional studies in large cohorts are needed to clarify the relationship between GWG, both in absolute terms and relative to recommendation (which depend on BMI), and risk of asthma in the offspring.

The prevalence of GDM has increased in the last decades in relation to a similar trend in the prevalence of maternal obesity, among other factors [20]. The association between maternal diabetes and offspring asthma have been scarcely studied, and inconsistent results have been reported [10], with either a positive association [21–24] or an absence of association [13,25,26]. Moreover, most studies were limited by the absence of distinction between maternal diabetes before or during pregnancy [21–25], the lack of adjustment for important confounders, such as maternal BMI [22–24,26], or the relatively low number of women with GDM [13,27]. In addition, although a few studies have included early pregnancy BMI as a confounder in the association between GDM and childhood asthma [13,27,28], none, to our knowledge, has examined its role as a potential effect modifier.

Finally, differences in the association between maternal BMI and asthma by sex and allergy status have been reported [13,29,30]. Asthma is a heterogeneous disease, and different asthma phenotypes (e.g., allergic and non-allergic asthma) have been shown to have different underlying mechanisms and etiologies [31]. However, these hypotheses have scarcely been examined for the association between GWG or GDM and childhood asthma [13].

In a large cohort of mothers and their children in the greater Boston (USA) area, we aimed to investigate the association of GWG and GDM with incidence of asthma in the offspring. We examined these associations according to early pregnancy maternal BMI, offspring sex, and allergy status.

## 2. Materials and Methods

### 2.1. Study Population

The Massachusetts General Hospital (MGH) Maternal-Child Cohort (MMCC) includes 48,114 mothers and their children born at MGH between 1998 and 2015, and who received care at an affiliated hospital in the child's first years of life. The MMCC was created by linking the MGH Maternal Health Cohort and the MGH Birth Cohort. The data were obtained by linking MGH electronic health records (EHR) for prenatal visits and delivery with data from Partners HealthCare (now Mass General Brigham (MGB)) EHR for clinical data in children. The MGB Human Research Committee approved the study and waived the requirement for informed consent.

The current analysis includes mothers who received care from 1998–2010, whose children were at least 5 years old before the 4th quarter of 2015 and had at least one health care encounter (e.g., healthy child visit) between ages 3 and 5 years. For internal validity, we excluded data beginning in the 4th quarter of 2015 due to the transition from the International Classification of Diseases (ICD)-9 to ICD-10 codes in EHR; accordingly, all outcomes were defined using ICD-9 codes.

### 2.2. Asthma and Allergies

The main outcome was the incidence of asthma by age 5 years. Asthma was defined as in prior work [32] by a primary billing diagnosis (ICD-9 493.xx) on or after age 3 years but before age 5 years, or ≥2 asthma medication "events" within a 12-month period beginning on or after age 3 years but before age 5 years. "Events" are defined as coded medication entries in either inpatient or outpatient locations. Asthma medications include short-acting bronchodilators, inhaled corticosteroids, inhaled corticosteroids plus long-acting
bronchodilators, and oral leukotriene modifiers. In a validation study performed on a subset of children with this asthma definition, a board-certified allergist/immunologist confirmed 92% of asthma cases on the basis of chart review [32].

Allergies were defined by the presence of atopic dermatitis or allergic rhinitis. We did not include food allergy because it cannot be accurately evaluated based on ICD-9 codes. Atopic dermatitis was defined by ≥2 billing codes for atopic dermatitis (ICD-9 691.8) or dermatitis due to food taken internally by age 3 years (ICD-9 693.1) [32]. Allergic rhinitis was defined by ≥2 billing codes for ICD-9 code 477.x. Among children with asthma, “allergic asthma” and “non-allergic asthma” were defined on the basis of the presence or absence of atopic dermatitis or allergic rhinitis.

## 2.3. Prenatal Exposures

The primary exposures of interest were GWG and GDM. We also studied a closely related factor, maternal early pregnancy BMI, to check the consistency of the findings from the MMCC cohort with the literature. Information on maternal height and early pregnancy weight, used to evaluate early pregnancy BMI (kg/m²) as well as GWG and GDM, were obtained from EHR at prenatal visits and delivery.

Early pregnancy weight was evaluated, on average, at 12 weeks of gestation. Early pregnancy BMI was classified according to the following categories: <20, 20–22.4 (reference category), 22.5–24.9, 25–29.9, and ≥30 kg/m² [11,13], in order to explore potential non-linear effects of maternal BMI on childhood asthma. Maternal GWG (lb) was studied first in absolute values, classified according to the following categories: <15, 15–24, 25–34 (reference category), 35–44, and ≥45 pounds gained, and then relative to early pregnancy BMI, according to the U.S. Institute of Medicine (IOM) recommendations (under recommended weight gain, meets recommended weight gain, or over recommended weight gain). The current IOM GWG guidelines (2009) recommend 28–40 lb of weight gain for underweight (<18.5 kg/m²), 25–35 lb for normal weight (18.5–24.9 kg/m²), 15–25 lb for overweight (25.0–29.9 kg/m²), and 11–20 lb for obese (≥30.0 kg/m²) pregnant women [33].

Women underwent a GDM screening test using a non-fasting 50-gram glucose loading test (GLT) between weeks 24 and 28 gestation, followed by a diagnostic fasting 100-gram 3 h oral glucose tolerance test (OGTT) if the GLT result was ≥140 mg/dl. Pregnancies were classified as having GDM if ≥2 OGTT values met or exceeded thresholds. In our analyses, GDM was defined according to (1) the Carpenter–Coustan criteria [34] and (2) the National Diabetes Data Group (NDDG, stricter) criteria. The latter was in clinical use at MGH during the study period. Pregnancies without complete GDM testing data according to the clinical protocol (e.g., missing an OGTT if GLT was abnormal, incomplete OGTT screening) were excluded from the analysis. Women known to have pre-existing diabetes were not screened for GDM and are, thus, excluded from the GDM analyses.

## 2.4. Covariates

We considered the following maternal characteristics in multivariable models: age at delivery, history of asthma, race/ethnicity, smoking status (3 months prior to pregnancy or during pregnancy), mode of delivery (cesarean versus vaginal), and insurance status at birth (surrogate for socio-economic condition). Adjustment for self-reported maternal education was also considered, and results were similar (data not shown). Analyses of GWG and GDM were further adjusted for maternal early pregnancy BMI (kg/m²). As induction of labor at 38–39 weeks of gestation is indicated in women with GDM and may affect asthma risk, we adjusted for gestational age at delivery (continuous) in a sensitivity analysis.

## 2.5. Statistical Analyses

The associations of prenatal exposures with asthma in the offspring, overall and by allergy subtypes, were evaluated using logistic regression, adjusted for maternal characteristics, with generalized estimating equations (GEE) to take into account familial dependence among children of the same mother. Children with non-allergic asthma and with allergic
asthma were compared with children without asthma. We performed analyses stratified by sex for all prenatal exposures and stratified by maternal early pregnancy BMI (20–24.9 vs. ≥25 kg/m²) for GWG and GDM, with test for interactions in multivariable models. A two-sided *p* < 0.05 was considered statistically significant. All analyses were run using SAS V9.4 (SAS Institute, Cary, NC, USA).

## 3. Results

A total of 16,772 mother–child pairs were enrolled in the MMCC cohort in 1998–2010 and received care at a MGB hospital between ages 3 years and 5 years. After exclusions due to missing values for early pregnancy BMI (*n* = 384), GWG (*n* = 30), or covariates (*n* = 7), the analytic cohort for BMI and GWG analyses included 16,351 children. GDM status could not be ascertained in 1180 pregnancies; thus, the analytic cohort for GDM analyses included 15,171 children. Participants without complete GDM screening data differed on several characteristics, including early pregnancy BMI and GWG (Table S1).

In early pregnancy, 29% of the mothers had overweight BMI and 21% had obesity (Table 1). During pregnancy, 36% of the mothers met recommended weight gain, 40% gained more, and 24% gained less weight than recommended. GDM was observed in 710 (4.7%) pregnancies according to Carpenter–Coustan criteria and 441 (2.9%) according to the NDDG criteria. A total of 2306 children (14%) developed asthma by age 5 years, and, among them, 1525 (66%) had non-allergic asthma and 781 (34%) had allergic asthma.

<table><caption>**Table 1.** Maternal and child's characteristics according to the child's asthma status.</caption><thead><tr><th></th><th>All (n = 16,351)</th><th>Child<br>without Asthma (n = 14,045)</th><th>Child with Asthma<br>(n = 2306)</th><th>p</th></tr></thead><tbody><tr><td><strong>Maternal characteristics</strong></td><td></td><td></td><td></td><td></td></tr><tr><td>Early pregnancy BMI, kg/m², %</td><td></td><td></td><td></td><td>&lt;0.0001</td></tr><tr><td>&lt;20.0</td><td>8.2</td><td>8.3</td><td>7.4</td><td></td></tr><tr><td>20.0–22.4</td><td>19.6</td><td>20.2</td><td>16.3</td><td></td></tr><tr><td>22.5–24.9</td><td>22.6</td><td>22.7</td><td>21.8</td><td></td></tr><tr><td>25–29.9</td><td>28.8</td><td>28.6</td><td>30.1</td><td></td></tr><tr><td>≥30</td><td>20.8</td><td>20.2</td><td>24.4</td><td></td></tr><tr><td>GWG, lb, %</td><td></td><td></td><td></td><td>0.02</td></tr><tr><td>&lt;15</td><td>13.8</td><td>13.5</td><td>16.0</td><td></td></tr><tr><td>15–24</td><td>25.3</td><td>25.4</td><td>24.8</td><td></td></tr><tr><td>25–34</td><td>34.0</td><td>34.1</td><td>33.0</td><td></td></tr><tr><td>35–44</td><td>19.3</td><td>19.5</td><td>18.3</td><td></td></tr><tr><td>≥45</td><td>7.6</td><td>7.5</td><td>7.9</td><td></td></tr><tr><td>GWG relative to recommendations, %</td><td></td><td></td><td></td><td>0.29</td></tr><tr><td>Under recommended weight gain</td><td>23.8</td><td>23.8</td><td>24.3</td><td></td></tr><tr><td>Meets recommended weight gain</td><td>36.4</td><td>36.6</td><td>34.9</td><td></td></tr><tr><td>Over recommended weight gain</td><td>39.8</td><td>39.6</td><td>40.8</td><td></td></tr><tr><td>GDM, Carpenter–Coustan criteria<br>(missing for 1180, 7%), %</td><td>4.7</td><td>4.5</td><td>5.8</td><td>0.01</td></tr><tr><td>GDM, National Diabetes Data Group<br>criteria (missing for 1180, 7%), %</td><td>2.9</td><td>2.7</td><td>4.1</td><td>0.0002</td></tr><tr><td>Cesarean delivery, %</td><td>28.3</td><td>27.7</td><td>32.2</td><td>&lt;0.0001</td></tr><tr><td>Nulliparous, %</td><td></td><td></td><td></td><td>0.24</td></tr><tr><td>Yes</td><td>47.3</td><td>47.4</td><td>46.7</td><td></td></tr><tr><td>No</td><td>49.6</td><td>49.6</td><td>49.7</td><td></td></tr><tr><td>Missing</td><td>3.1</td><td>3.0</td><td>3.6</td><td></td></tr><tr><td>Age at delivery, mean (SD)</td><td>30.3 (6.4)</td><td>30.4 (6.4)</td><td>30.0 (6.4)</td><td>0.01</td></tr><tr><td>Maternal race/ethnicity, %</td><td></td><td></td><td></td><td>&lt;0.0001</td></tr><tr><td>White</td><td>51.2</td><td>52.1</td><td>45.8</td><td></td></tr><tr><td>Black</td><td>7.6</td><td>7.4</td><td>8.9</td><td></td></tr><tr><td>Hispanic</td><td>20.2</td><td>19.9</td><td>22.2</td><td></td></tr><tr><td>Asian</td><td>7.6</td><td>7.6</td><td>7.3</td><td></td></tr><tr><td>None of the above</td><td>13.4</td><td>13.0</td><td>15.8</td><td></td></tr></tbody></table>
<table><caption>**Table 1.** *Cont.*</caption><thead><tr><th></th><th>All (n = 16,351)</th><th>Child<br>without Asthma (n = 14,045)</th><th>Child with Asthma<br>(n = 2306)</th><th>p</th></tr></thead><tbody><tr><td>Maternal asthma, %</td><td>8.0</td><td>7.2</td><td>12.8</td><td>&lt;0.0001</td></tr><tr><td>Smoking status (smoker 3 months prior<br>to pregnancy or during pregnancy), %</td><td>7.8</td><td>7.7</td><td>8.3</td><td>0.30</td></tr><tr><td>Insurance status at birth, %</td><td></td><td></td><td></td><td>&lt;0.0001</td></tr><tr><td>Private</td><td>55.0</td><td>56.0</td><td>49.2</td><td></td></tr><tr><td>Public</td><td>34.8</td><td>33.7</td><td>41.4</td><td></td></tr><tr><td>Limited</td><td>6.6</td><td>6.6</td><td>6.3</td><td></td></tr><tr><td>Other</td><td>3.6</td><td>3.7</td><td>3.0</td><td></td></tr><tr><td><strong>Child's characteristics</strong></td><td></td><td></td><td></td><td></td></tr><tr><td>Female, %</td><td>47.9</td><td>49.2</td><td>39.9</td><td>&lt;0.0001</td></tr><tr><td>Birth weight, lb, %</td><td></td><td></td><td></td><td>&lt;0.0001</td></tr><tr><td>&lt;5.5</td><td>6.9</td><td>6.5</td><td>9.7</td><td></td></tr><tr><td>5.5-6.9</td><td>26.7</td><td>26.8</td><td>25.6</td><td></td></tr><tr><td>7.0-8.4</td><td>48.8</td><td>49.1</td><td>47.1</td><td></td></tr><tr><td>8.5-9.9</td><td>16.1</td><td>16.2</td><td>15.6</td><td></td></tr><tr><td>≥10</td><td>1.5</td><td>1.4</td><td>2.0</td><td></td></tr><tr><td>Gestational age at birth, weeks, %</td><td></td><td></td><td></td><td>&lt;0.0001</td></tr><tr><td>&lt;32</td><td>1.1</td><td>0.8</td><td>2.7</td><td></td></tr><tr><td>32-36</td><td>6.0</td><td>5.7</td><td>7.7</td><td></td></tr><tr><td>≥37</td><td>92.9</td><td>93.5</td><td>89.6</td><td></td></tr><tr><td>Atopic dermatitis, %</td><td>9.3</td><td>8.0</td><td>17.7</td><td>&lt;0.0001</td></tr><tr><td>Allergic rhinitis, %</td><td>10.4</td><td>8.2</td><td>24.3</td><td>&lt;0.0001</td></tr></tbody></table>

BMI—body mass index; GDM—gestational diabetes mellitus; GWG—gestational weight gain; % missing values is displayed for variables with >3% missing values.

In multivariable models (Table 2), early pregnancy BMI was positively associated with higher risk of offspring asthma, with an increasing trend across BMI categories (BMI 22.5–24.9: odds ratio (OR) 1.17, 95% confidence interval (CI) 1.01–1.35; BMI 25–29.9: OR 1.21, CI 1.05–1.39; BMI ≥ 30: OR 1.29, CI 1.12–1.50; *p*-trend < 0.001). A similar trend was observed for non-allergic asthma, although associations were attenuated. More pronounced associations were observed for allergic asthma, with ORs > 1.35 for all BMI categories ≥22.5. Lower early pregnancy BMI (<20) was also associated with increased risk of allergic asthma (OR 1.52, CI 1.12–2.06) compared with the reference category (BMI 20.0–22.4). Associations between maternal BMI and asthma were similar in boys and girls (Table S2).

Overall, no association was found between GWG, in absolute values or relative to recommendations, and offspring asthma. For allergic asthma, we observed a positive association with the highest GWG category (>45 lb: OR 1.31, 95% CI 1.00–1.71; Table 2). Results were similar in boys and girls (Table S2) and in analyses stratified according to early pregnancy BMI (Table S3).

GDM was positively associated with offspring asthma, regardless of the definition used (Carpenter-Coustan criteria: OR 1.24, 95% CI 1.01–1.53; NDDG criteria: OR 1.46, CI 1.14–1.88; Table 3). Using the NDDG criteria, associations were more pronounced for non-allergic asthma (OR 1.57, CI 1.17–2.11) than for allergic asthma (OR 1.23, CI 0.81–1.85), although this difference was not statistically significant (*p* = 0.35). In sensitivity analyses with further adjustment for gestational age, associations were attenuated but remained significant for the NDDG criteria (Table S4). Analyses stratified by child's sex did not show any significant differences (*p*-interaction > 0.50) (Table S5). In analyses stratified by early pregnancy BMI (Figure 1), associations between GDM and offspring asthma were observed among those with early pregnancy BMI between 20 and 24.9 kg/m² (Carpenter-Coustan criteria: OR 1.64, 95% CI 1.09–2.45; NDDG criteria: OR 2.31, CI 1.46–3.65), while ORs were close to null among women with early pregnancy BMI ≥ 25 kg/m² (*p*-interaction Carpenter-Coustan criteria: 0.09; NDDG criteria: 0.02). This difference was observed for non-allergic asthma (*p*-interaction < 0.05) but not for allergic asthma (*p*-interaction > 0.80).
<table><caption>**Table 2.** Associations of maternal BMI and GWG with incidence of allergic and non-allergic asthma in childhood.</caption><thead><tr><th rowspan="2"></th><th colspan="2">All Asthma</th><th colspan="2">Non-Allergic Asthma</th><th colspan="2">Allergic Asthma</th></tr><tr><th>No. of Cases</th><th>OR (95% CI)</th><th>No. of Cases</th><th>OR (95% CI)</th><th>No. of Cases</th><th>OR (95% CI)</th></tr></thead><tbody><tr><td>Maternal early pregnancy BMI, kg/m<sup>2</sup></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>&lt;20.0</td><td>170</td><td>1.08 (0.89–1.32)</td><td>97</td><td>0.89 (0.69–1.13)</td><td>73</td><td><strong>1.52 (1.12–2.06)</strong></td></tr><tr><td>20.0–22.4 (ref.)</td><td>375</td><td>1</td><td>261</td><td>1</td><td>114</td><td>1</td></tr><tr><td>22.5–24.9</td><td>502</td><td><strong>1.17 (1.01–1.35)</strong></td><td>328</td><td>1.08 (0.91–1.29)</td><td>174</td><td><strong>1.35 (1.06–1.72)</strong></td></tr><tr><td>25–29.9</td><td>695</td><td><strong>1.21 (1.05–1.39)</strong></td><td>451</td><td>1.11 (0.94–1.31)</td><td>244</td><td><strong>1.44 (1.14–1.81)</strong></td></tr><tr><td>≥30</td><td>564</td><td><strong>1.29 (1.12–1.50)</strong></td><td>388</td><td><strong>1.24 (1.04–1.48)</strong></td><td>176</td><td><strong>1.39 (1.09–1.79)</strong></td></tr><tr><td><em>p-trend</em></td><td></td><td><strong>0.001</strong></td><td></td><td><strong>0.002</strong></td><td></td><td>0.16</td></tr><tr><td>Maternal GWG (lb) †</td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>&lt;15</td><td>369</td><td>1.04 (0.90–1.21)</td><td>269</td><td>1.12 (0.94–1.32)</td><td>100</td><td>0.88 (0.68–1.13)</td></tr><tr><td>15–24</td><td>573</td><td>0.90 (0.84–1.06)</td><td>363</td><td>0.89 (0.77–1.02)</td><td>210</td><td>1.05 (0.87–1.28)</td></tr><tr><td>25–34 (ref.)</td><td>760</td><td>1</td><td>507</td><td>1</td><td>253</td><td>1</td></tr><tr><td>35–44</td><td>422</td><td>0.97 (0.85–1.10)</td><td>281</td><td>0.95 (0.82–1.12)</td><td>141</td><td>0.97 (0.78–1.20)</td></tr><tr><td>≥45</td><td>182</td><td>1.01 (0.85–1.21)</td><td>105</td><td>0.86 (0.69–1.08)</td><td>77</td><td><strong>1.31 (1.00–1.71)</strong></td></tr><tr><td><em>p-trend</em></td><td></td><td>0.84</td><td></td><td>0.20</td><td></td><td>0.14</td></tr><tr><td>GWG relative to recommendations †</td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>Under recommendations</td><td>561</td><td>1.04 (0.92–1.17)</td><td>380</td><td>1.04 (0.91–1.20)</td><td>181</td><td>1.03 (0.84–1.25)</td></tr><tr><td>Meets recommendations</td><td>805</td><td>1</td><td>540</td><td>1</td><td>265</td><td>1</td></tr><tr><td>Over recommendations</td><td>940</td><td>1.01 (0.91–1.13)</td><td>605</td><td>0.96 (0.85–1.09)</td><td>335</td><td>1.11 (0.94–1.32)</td></tr></tbody></table>

BMI—body mass index; GWG—gestational weight gain; OR—odds ratio; CI—confidence interval. Analyses were adjusted for maternal age at delivery, asthma, maternal race/ethnicity, smoking status, insurance status at birth, and mode of delivery (C-section). † Analyses were further adjusted for maternal early pregnancy BMI. Results in boldface are statistically significant.

<figure><img src="image_5.png" /><figcaption>**Figure 1.** Associations of GDM with incidence of allergic and non-allergic asthma in childhood according to maternal early pregnancy BMI. BMI—body mass index; GDM—gestational diabetes mellitus; OR—odds ratio; CI—confidence interval. Analyses were adjusted for age at delivery, asthma, maternal race/ethnicity, smoking status, insurance status at birth, and mode of delivery (C-section). *p*-values for interaction are displayed on the upper part of the figure.</figcaption></figure>
<table><caption>**Table 3.** Associations of GDM with incidence of allergic and non-allergic asthma in childhood.</caption><thead><tr><th rowspan="2"></th><th colspan="2">All Asthma</th><th colspan="2">Non-Allergic Asthma</th><th colspan="2">Allergic Asthma</th></tr><tr><th>No. of Cases</th><th>OR (95% CI)</th><th>No. of Cases</th><th>OR (95% CI)</th><th>No. of Cases</th><th>OR (95% CI)</th></tr></thead><tbody><tr><td>GDM, Carpenter-Coustan criteria</td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>No</td><td>1980</td><td>1</td><td>1301</td><td>1</td><td>679</td><td>1</td></tr><tr><td>Yes</td><td>122</td><td><strong>1.24 (1.01–1.53)</strong></td><td>78</td><td>1.21 (0.93–1.56)</td><td>44</td><td>1.28 (0.93–1.76)</td></tr><tr><td>GDM, National Diabetes Data Group criteria</td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>No</td><td>2015</td><td>1</td><td>1318</td><td>1</td><td>697</td><td>1</td></tr><tr><td>Yes</td><td>87</td><td><strong>1.46 (1.14–1.88)</strong></td><td>61</td><td><strong>1.57 (1.17–2.11)</strong></td><td>26</td><td>1.23 (0.81–1.85)</td></tr></tbody></table>

BMI—body mass index; GDM—gestational diabetes mellitus; OR—odds ratio; CI—confidence interval. Analyses were adjusted for maternal early pregnancy BMI, age at delivery, asthma, maternal race/ethnicity, smoking status, insurance status at birth, mode of delivery (C-section). Results in boldface are statistically significant.

**4. Discussion**

In a cohort of >16,000 mother–child pairs, we examined the impact of several prenatal exposures, including maternal early pregnancy BMI, GWG, and GDM, on the risk of asthma in the offspring.

Our findings confirmed a positive association between early pregnancy overweight or obesity and higher risk of asthma in the offspring, and we found an additional association between low (<20 kg/m²) early pregnancy BMI and allergic asthma. While an association between high maternal BMI and increased risk of asthma in the offspring is well-established, the latest meta-analysis reported an absence of association between low maternal BMI (underweight) and asthma risk [9]. However, allergic and non-allergic asthma subtypes were not distinguished. In the current study, low maternal BMI was associated only with allergic asthma in the offspring. Few studies have examined allergic phenotypes in relation to maternal BMI. In a previous U.S. study, we found no association between low maternal pre-pregnancy BMI and either allergic nor non-allergic asthma in the child [13]. A study of Danish women with asthma found that maternal underweight was associated with increased risk of wheeze in the offspring [35]. This study [35] and a few others [17,18,36] also found an association with increased risk of atopic dermatitis. It is, thus, possible that an association of low maternal BMI with atopic diseases drives the results currently observed for allergic asthma.

Regarding GWG, the current study does not support any clear association with offspring asthma. Indeed, we found no association between GWG and asthma, except for very high weight gain (≥45 lb) and allergic asthma. Inconsistent findings have been reported in the literature on this question: while the risk of childhood asthma or wheeze has been reported to modestly increase with both low and high GWG values in a meta-analysis of six studies [9], different findings have since been reported. In a Chinese study of 15,145 mother–child pairs, a low GWG, including GWG below IOM recommendations, was associated with a lower risk of asthma [16]. In a Canadian study using linkage of birth registry and health administrative databases to form a cohort of 214,017 children, no association was found between GWG and asthma [17]. The authors from the latter study noted that a null or modest association between GWG and offspring asthma, in contrast with the well-acknowledged pre-pregnancy BMI–offspring asthma association, is consistent with recent findings of a stronger impact of pre-pregnancy BMI than GWG on inflammatory markers generally thought to influence asthma risk [17].

In the current study, we found that GDM was associated with increased risk of asthma in the offspring. Few studies have examined the association between maternal diabetes and the risk of childhood asthma. A meta-analysis published in 2021 [10], including only four studies on maternal diabetes, found a modest but significantly increased risk of asthma in the offspring (meta-RR: 1.13, 95% CI 1.01–1.27). However, most previous studies, including those in the meta-analysis, examined all types of maternal diabetes together and not specif-
ically GDM [10,23,24,26]. Distinguishing the type of diabetes is important, as they vary in terms of etiology, severity, management, and health consequences [37]. Moreover, they have been suggested to be differentially associated with offspring wheezing/asthma [28,38]. Another limitation from previous studies was the absence of adjustment for maternal BMI, an important confounder [22–24,26]. A few more recent studies with stronger designs and methodology inform about a potential role of GDM in childhood asthma. In a cohort of 97,554 children aged 5 years or older in Southern California using EHR data, a 17% increased risk of asthma in children was reported for mothers with GDM requiring medication; however, the association was attenuated (HR: 1.10,96% CI: 0.99–1.21, *p* = 0.07) after adjustment for maternal pre-pregnancy BMI and GWG [28]. Similarly, a very large Danish registry-based cohort examining early-life wheezing phenotypes reported associations between GDM and early onset wheeze, which were no longer significant after adjustment for pre-pregnancy BMI [38]. In the latter study, relatively sensitive definitions for wheezing phenotypes were used (wheezing treatment at least once during 0–3 years and/or 4–6 years), which are likely to capture both early life transient wheezing and childhood asthma. In a smaller U.S. cohort (n = 1107, of which 62 women had GDM), GDM was associated with a significant, two-fold increased risk of asthma at age 4 years, defined by questionnaire [27]. Our findings, thus, make an important addition to the limited amount of evidence on the relationship between GDM and child asthma.

To the best of our knowledge, we are the first to examine the association between GDM and offspring asthma according to maternal pre-pregnancy BMI and child allergic status. A stronger association between GDM and offspring asthma was observed among women with normal early pregnancy BMI, in particular, for non-allergic asthma. Asthma is a heterogeneous disease, with multiple determinants and associated mechanisms. The current findings suggest a role of predominantly nonallergic pathways in the association between maternal GDM and child asthma, but this needs to be confirmed. The absence of association among women who are overweight or obese may be due to the already increased risk of offspring asthma conferred by maternal overweight or obesity, which may not be further increased by GDM. The different associations across the weight spectrum may also be related to distinct mechanisms of GDM in those with different BMI. Recent data also emphasize GDM heterogeneity [37]. Among identified GDM physiologic subtypes, only insulin-resistant GDM (approximately half of the cases) was associated with higher BMI. [37] Women with insulin-deficient GDM had BMIs similar to those of women with normal glucose tolerance. While mechanisms linking different GDM subtypes to adverse maternal and child outcomes still need to be elucidated, considering the heterogeneity of GDM when studying its causes and consequences is encouraged [37]. Interestingly, a nutritional intervention promoting a Mediterranean diet in pregnant women, first shown to reduce the risk of GDM in a randomized control trial, has been subsequently associated with a lower risk of hospitalization for asthma or bronchiolitis by age 2 years in children [7]. This finding was observed only in women with pre-pregnancy BMI <25 kg/m². Whether dietary changes included in prevention and management of GDM may also be relevant for the prevention of childhood asthma merits further investigation [6].

The strengths of the current study include the large population size and the comprehensive assessment of prenatal exposures. Maternal BMI and GWG were based on objective measurements performed during prenatal visits and, thus, do not rely on mother's recall, which may be subject to bias [39]. Maternal weight was first measured at the first prenatal visit, on average, at 12 weeks of pregnancy. Thus, an objective assessment of pre-pregnancy BMI was not available. We acknowledge that this limitation makes it more difficult to distinguish the respective role of pre-pregnancy BMI and GWG in childhood asthma. However, weight gain is generally minimal in the first weeks of pregnancy [33], and early pregnancy BMI can be considered as a good surrogate for pre-pregnancy BMI. Standardized procedures were used for the screening and diagnosis of GDM, and gold-standard laboratory data were available. Our results were robust when using different OGTT thresholds (Carpenter–Coustan and NDDG criteria), and associations were stronger when using the
threshold that captures more severe hyperglycemia (NDDG criteria). With the latter thresh-
old, in clinical use during the study period, associations remained significant after further
adjustment for gestational age, controlling for a potential impact of early labor induction
indicated in women with diagnosed GDM. Asthma definition was also based on medical
records rather than parental report. We acknowledge that asthma is difficult to diagnose
before age 5 years [31]. However, in this cohort of young children, we used a specific and
validated [32] asthma definition, based on billing diagnosis and repeated prescription of
asthma medication over a 12-month period between ages 3 and 5 years, in order to avoid
inclusion of children with transient wheezing in the asthma group. While our results need
to be confirmed with longer follow-up data, previous studies that examined age at asthma
onset found that associations with maternal BMI tended to be stronger for early onset
asthma [13,40], suggesting that early childhood is a relevant window to examine asthma
onset in relation to prenatal exposures. We relied on ICD-9-coded billing diagnoses to
define allergy status, which likely resulted in a relatively specific definition of “allergic
asthma”. Our results regarding allergic status need to be confirmed in studies with more
accurate data on allergic sensitization (e.g., IgE levels). Finally, information regarding some
risk factors for childhood asthma, such as the exact number of siblings, was not available
in this cohort. However, parity status (nulliparous yes/no) was not associated with child
asthma, suggesting that the number of siblings is unlikely to be a strong confounder in
this population.

**5. Conclusions**

In summary, in addition to the well-known role of maternal adiposity during preg-
nancy as a risk factor for asthma in the offspring, we report an additional association
between low (<20 kg/m²) early pregnancy BMI and allergic asthma. We found no consis-
tent association between GWG and asthma. Finally, we found that GDM was associated
with increased risk of asthma in the offspring. The latter association was particularly strong
among women with normal early pregnancy BMI (20–24.9 kg/m²). Possible differences
according to allergy status were suggested in these relationships, which deserve further
investigation, as they provide clues to potential underlying mechanisms. Our findings
support the strengthening of interventions targeted toward a healthier pregnancy and re-
duction of adverse perinatal outcomes, as they may also be helpful for primary prevention
of childhood asthma.

**Supplementary Materials:** The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/nu14235188/s1, Table S1: Comparison of maternal and child's characteristics in mother–child pairs with or without missing data for GDM; Table S2: Associations of maternal BMI and GWG with incidence of asthma in childhood according to the child's sex; Table S3: Associations of GWG with incidence of asthma in childhood according to maternal early pregnancy BMI; Table S4: Associations of GDM with incidence of allergic and non-allergic asthma in childhood, with further adjustment for gestational age; Table S5: Associations of GDM with incidence of asthma in childhood according to the child's sex.

**Author Contributions:** Conceptualization, O.D. and C.A.C.J.; data curation, K.J., S.H., C.P. and C.A.C.J.; formal analysis, M.K.F.; investigation, O.D., A.C.A., K.J., S.H., C.P., and C.A.C.J.; methodology, O.D., A.C.A., M.K.F., C.P. and C.A.C.J.; resources, C.P. and C.A.C.J.; supervision, C.A.C.J.; writing—original draft, O.D.; writing—review and editing, A.C.A., M.K.F., K.J., S.H., C.P. and C.A.C.J. All authors have read and agreed to the published version of the manuscript.

**Funding:** A.C. Arroyo was supported by the National Institutes of Health (grant no. R25AI147369). The content of this manuscript is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

**Institutional Review Board Statement:** The Mass General Brigham Human Research Committee approved the study.

**Informed Consent Statement:** The Mass General Brigham Human Research Committee waived the requirement for informed consent as data were solely obtained by linking electronic health records.
**Data Availability Statement:** The data that support the findings of this study are available on reasonable request from the corresponding author. The data are not publicly available due to privacy or ethical restrictions.

**Acknowledgments:** The authors thank Janice A. Espinola and Sijia Li for data management and statistical analysis and Ashley F. Sullivan for project coordination.

**Conflicts of Interest:** The authors declare no conflict of interest.

## References

1. Ferrante, G.; La Grutta, S. The Burden of Pediatric Asthma. *Front. Pediatr.* **2018**, *6*, 186. [CrossRef] [PubMed]

2. Centers for Disease Control and Prevention. 2019 National Health Interview Survey (NHIS) Data. Available online: https://www.cdc.gov/asthma/nhis/2019/data.htm (accessed on 23 February 2022).

3. Tai, A.; Tran, H.; Roberts, M.; Clarke, N.; Gibson, A.-M.; Vidmar, S.; Wilson, J.; Robertson, C.F. Outcomes of childhood asthma to the age of 50 years. *J. Allergy Clin. Immunol.* **2014**, *133*, 1572–1578.e3. [CrossRef] [PubMed]

4. Bobolea, I.; Arismendi, E.; Valero, A.; Agustí, A. Early Life Origins of Asthma: A Review of Potential Effectors. *J. Investig. Allergy Clin. Immunol.* **2019**, *29*, 168–179. [CrossRef]

5. Camargo, C.A. Gestational weight gain and offspring asthma: A novel opportunity for primary prevention research. *Clin. Exp. Allergy* **2015**, *45*, 544–546. [CrossRef] [PubMed]

6. Bédard, A.; Li, Z.; Ait-Hadad, W.; Camargo, C.A.; Leynaert, B.; Pison, C.; Dumas, O.; Varraso, R. The Role of Nutritional Factors in Asthma: Challenges and Opportunities for Epidemiological Research. *Int. J. Environ. Res. Public Health* **2021**, *18*, 3013. [CrossRef]

7. Melero, V.; Assaf-Balut, C.; De La Torre, N.G.; Jiménez, I.; Bordiú, E.; Del Valle, L.; Valerio, J.; Familiar, C.; Durán, A.; Runkle, I.; et al. Benefits of Adhering to a Mediterranean Diet Supplemented with Extra Virgin Olive Oil and Pistachios in Pregnancy on the Health of Offspring at 2 Years of Age. Results of the San Carlos Gestational Diabetes Mellitus Prevention Study. *J. Clin. Med.* **2020**, *9*, 1454. [CrossRef]

8. Forno, E.; Young, O.M.; Kumar, R.; Simhan, H.; Celedón, J.C. Maternal Obesity in Pregnancy, Gestational Weight Gain, and Risk of Childhood Asthma. *Pediatrics* **2014**, *134*, e535–e546. [CrossRef]

9. Liu, S.; Zhou, B.; Wang, Y.; Wang, K.; Zhang, Z.; Niu, W. Pre-pregnancy Maternal Weight and Gestational Weight Gain Increase the Risk for Childhood Asthma and Wheeze: An Updated Meta-Analysis. *Front. Pediatr.* **2020**, *8*, 134. [CrossRef]

10. Li, Z.; Yu, M.; Wang, P.; Qian, H.; Fan, Y.; Li, X.; Xu, Q.; Wang, X.; Wang, X.; Lu, C. Association between maternal diabetes mellitus and allergic diseases in children—A systematic review and meta-analysis. *Pediatr. Allergy Immunol.* **2021**, *32*, 880–891. [CrossRef] [PubMed]

11. Leermakers, E.T.M.; Der Voort, A.M.S.-V.; Gaillard, R.; Hofman, A.; De Jongste, J.C.; Jaddoe, V.W.; Duijts, L. Maternal weight, gestational weight gain and preschool wheezing: The Generation R Study. *Eur. Respir. J.* **2013**, *42*, 1234–1243. [CrossRef]

12. Harpsøe, M.C.; Basit, S.; Bager, P.; Wohlfahrt, J.; Benn, C.S.; Nøhr, E.A.; Linneberg, A.; Jess, T. Maternal obesity, gestational weight gain, and risk of asthma and atopic disease in offspring: A study within the Danish National Birth Cohort. *J. Allergy Clin. Immunol.* **2012**, *131*, 1033–1040. [CrossRef] [PubMed]

13. Dumas, O.; Varraso, R.; Gillman, M.W.; Field, A.E.; Camargo, C.A. Longitudinal study of maternal body mass index, gestational weight gain, and offspring asthma. *Allergy* **2016**, *71*, 1295–1304. [CrossRef] [PubMed]

14. Halonen, M.; Lohman, I.C.; Stern, D.A.; Ellis, W.L.; Rothers, J.; Wright, A.L. Perinatal Tumor Necrosis Factor-α Production, Influenced by Maternal Pregnancy Weight Gain, Predicts Childhood Asthma. *Am. J. Respir. Crit. Care Med.* **2013**, *188*, 35–41. [CrossRef] [PubMed]

15. Polinski, K.J.; Liu, J.; Boghossian, N.S.; McLain, A.C. Maternal Obesity, Gestational Weight Gain, and Asthma in Offspring. *Prev. Chronic Dis.* **2017**, *14*, 199–204.e3. [CrossRef]

16. Chen, Y.; Zhu, J.; Lyu, J.; Xia, Y.; Ying, Y.; Hu, Y.; Qu, J.; Tong, S.; Li, S. Association of Maternal Prepregnancy Weight and Gestational Weight Gain With Children's Allergic Diseases. *JAMA Netw. Open* **2020**, *3*, e2015643. [CrossRef]

17. Srugo, S.A.; Fell, D.B.; Corsi, D.J.; Fakhraei, R.; Guo, Y.; Gaudet, L.M. Examining the role of pre-pregnancy weight and gestational weight gain in allergic disease development among offspring: A population-based cohort study in Ontario, Canada. *Paediatr. Périnat. Epidemiol.* **2021**, *36*, 144–155. [CrossRef]

18. Drucker, A.M.; Pope, E.I.; Field, A.E.; Qureshi, A.A.; Dumas, O.; Camargo, C.A. Association Between Maternal Pre-Pregnancy Body Mass Index, Gestational Weight Gain, and Offspring Atopic Dermatitis: A Prospective Cohort Study. *J. Allergy Clin. Immunol. Pract.* **2018**, *7*, 96–102.e2. [CrossRef] [PubMed]

19. Deputy, N.; Sharma, A.; Kim, S.; Hinkle, S. Prevalence and Characteristics Associated With Gestational Weight Gain Adequacy. *Obstet. Gynecol.* **2015**, *125*, 773–781. [CrossRef] [PubMed]

20. Lavery, J.A.; Friedman, A.M.; Keyes, K.M.; Wright, J.D.; Ananth, C.V. Gestational diabetes in the United States: Temporal changes in prevalence rates between 1979 and 2010. *BJOG Int. J. Obstet. Gynaecol.* **2017**, *124*, 804–813. [CrossRef]

21. Rusconi, F.; Galassi, C.; Forastiere, F.; Bellasio, M.; De Sario, M.; Ciccone, G.; Brunetti, L.; Chellini, E.; Corbo, G.; La Grutta, S.; et al. Maternal Complications and Procedures in Pregnancy and at Birth and Wheezing Phenotypes in Children. *Am. J. Respir. Crit. Care Med.* **2007**, *175*, 16–21. [CrossRef]
22. Nasreen, S.; Wilk, P.; Mullowney, T.; Karp, I. The effect of gestational diabetes mellitus on the risk of asthma in offspring. *Ann. Epidemiol.* **2021**, *57*, 7–13. [CrossRef] [PubMed]

23. Azad, M.B.; Becker, A.B.; Kozyrskyj, A.L. Association of maternal diabetes and child asthma. *Pediatr. Pulmonol.* **2012**, *48*, 545–552. [CrossRef]

24. Haataja, P.; Korhonen, P.; Ojala, R.; Hirvonen, M.; Paassilta, M.; Gissler, M.; Luukkaala, T.; Tammerla, O. Asthma and Atopic Dermatitis in Children Born Moderatly and Late Preterm. *Eur. J. Pediatr.* **2016**, *175*, 799–808. [CrossRef]

25. Zugna, D.; Galassi, C.; Annesi-Maesano, I.; Baiz, N.; Barros, H.; Basterrechea, M.; Correia, S.; Duijts, L.; Esplugues, A.; Fantini, M.P.; et al. Maternal complications in pregnancy and wheezing in early childhood: A pooled analysis of 14 birth cohorts. *Int. J. Epidemiol.* **2015**, *44*, 199–208. [CrossRef] [PubMed]

26. Zamstein, O.; Sheiner, E.; Wainstock, T.; Landau, D.; Walfisch, A. Maternal gestational diabetes and long-term respiratory related hospitalizations of the offspring. *Diabetes Res. Clin. Pract.* **2018**, *140*, 200–207. [CrossRef] [PubMed]

27. Adgent, M.A.; Gebretsadik, T.; Reedus, J.; Graves, C.; Garrison, E.; Bush, N.; Davis, R.; LeWinn, K.Z.; Tylavsky, F.; Carroll, K.N. Gestational diabetes and childhood asthma in a racially diverse US pregnancy cohort. *Pediatr. Allergy Immunol.* **2021**, *32*, 1190–1196. [CrossRef]

28. Martinez, M.P.; Lin, J.; Chow, T.; Chung, J.; Wang, X.; Xiang, A.H. Maternal Gestational Diabetes and Type 2 Diabetes During Pregnancy and Risk of Childhood Asthma in Offspring. *J. Pediatr.* **2020**, *219*, 173–179.e1. [CrossRef]

29. Westberg, A.P.; Salonen, M.K.; von Bonsdorff, M.; Osmond, C.; Kajantie, E.; Eriksson, J.G.; Von Bondsdorff, M. Maternal adiposity in pregnancy and offspring asthma in adulthood. *Eur. Respir. J.* **2018**, *52*, 1801152. [CrossRef]

30. Lowe, A.J.; Ekéus, C.; Braback, L.; Rajaleid, K.; Forsberg, B.; Hjern, A. Impact of Maternal Obesity on Inhaled Corticosteroid Use in Childhood: A Registry Based Analysis of First Born Children and a Sibling Pair Analysis. *PLoS ONE* **2013**, *8*, e67368. [CrossRef]

31. Conrad, L.A.; Cabana, M.D.; Rastogi, D. Defining pediatric asthma: Phenotypes to endotypes and beyond. *Pediatr. Res.* **2020**, *90*, 45–51. [CrossRef]

32. Balekian, D.S.; Linnemann, R.W.; Hasegawa, K.; Thadhani, R.; Camargo, C.A. Cohort Study of Severe Bronchiolitis during Infancy and Risk of Asthma by Age 5 Years. *J. Allergy Clin. Immunol. Pract.* **2016**, *5*, 92–96. [CrossRef] [PubMed]

33. Rasmussen, K.M.; Yaktine, A.L. Weight Gain during Pregnancy: Reexamining the Guidelines; Institute of Medicine (US) and National Research Council (US) Committee to Reexamine IOM Pregnancy Weight Guidelines; National Academies Press (US): Washington, DC, USA, 2009.

34. Berggren, E.K.; Boggess, K.A.; Stuebe, A.M.; Funk, M.J. National Diabetes Data Group vs Carpenter-Coustan criteria to diagnose gestational diabetes. *Am. J. Obstet. Gynecol.* **2011**, *205*, 253.e1–253.e7. [CrossRef] [PubMed]

35. Ali, Z.; Jemec, G.B.E.; Ulrik, C.S. Associations between maternal and environmental exposures on atopic disease in the offspring of mothers with asthma. *Immun. Inflamm. Dis.* **2021**, *9*, 862–870. [CrossRef] [PubMed]

36. Chen, W.; Wang, L.; Yao, H.; Dai, H.; Zheng, R.; Zhang, W. Prepregnancy BMI, gestational weight gain and risk of childhood atopic dermatitis: A systematic review and meta-analysis. *Pediatr. Allergy Immunol.* **2021**, *32*, 892–904. [CrossRef] [PubMed]

37. Powe, C.E.; Hivert, M.-F.; Udler, M.S. Defining Heterogeneity Among Women With Gestational Diabetes Mellitus. *Diabetes* **2020**, *69*, 2064–2074. [CrossRef]

38. Liu, X.; Agerbo, E.; Li, J.; Dharmage, S.C.; Thomsen, R.W.; Olsen, J.; Munk-Olsen, T. Maternal pregestational or gestational diabetes and childhood wheezing: A population-based cohort study. *Allergy* **2018**, *73*, 2247–2250. [CrossRef]

39. Headen, I.; Cohen, A.K.; Mujahid, M.; Abrams, B. The accuracy of self-reported pregnancy-related weight: A systematic review. *Obes. Rev.* **2017**, *18*, 350–369. [CrossRef] [PubMed]

40. Ekström, S.; Magnusson, J.; Kull, I.; Lind, T.; Almqvist, C.; Melén, E.; Bergström, A. Maternal BMI in Early Pregnancy and Offspring Asthma, Rhinitis and Eczema up to 16 Years of Age. *Clin. Exp. Allergy* **2015**, *45*, 283–291. [CrossRef] [PubMed]