# DNA Metabarcoding to quantify the ecological impact of climate-induced forest diebacks in the Pyrenees

Paul Schmidt Yanez, Rodolphe Rougerie, Annie Bézier, Béatrice Courtial, Christophe Bouget, Laurent Larrieu, Douglas W. Yu, Michael T. Monaghan, Elisabeth A. Herniou, Carlos Lopez-Vaamonde

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## To cite this version:

Paul Schmidt Yanez, Rodolphe Rougerie, Annie Bézier, Béatrice Courtial, Christophe Bouget, et al. DNA Metabarcoding to quantify the ecological impact of climate-induced forest diebacks in the Pyrenees. 8. International Barcode of Life Conference (iBOL), Jun 2019, Trondheim, Norway. 16 p. hal-02787654

HAL Id: hal-02787654

https://hal.inrae.fr/hal-02787654

Submitted on 5 Jun 2020

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Distributed under a Creative Commons Attribution 4.0 International License
# DNA Metabarcoding to quantify the ecological impact of climate induced forest diebacks in the Pyrenees

## SIRE Lucas

Lucas.sire@univ-tours.fr

SCHMIDT YÁÑEZ Paul, ROUGERIE Rodolphe, BEZIER Annie, COURTIAL Béatrice, BOUGET Christophe, LARRIEU Laurent, YU Douglas W., MONAGHAN Michael T., HERNIOU Elisabeth A., LOPEZ-VAAMONDE Carlos
# Global changes and forestry management

## Global changes

"Climate change, insect infestations, drought..."

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Allen et al., 2015

## Salvage logging

"To harvest damaged trees in order to recover economic value otherwise lost."

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# Droughts induce silver fir (Abies alba) die-off in Pyrenees

Proportion of dominant trees with more than 25% of dead branches

More than 35%

From 20 to 35%

From 10 to 20%

Less than 10%

No tree in that case

Number of living and dominant trees

● More than 100

● From 50 to 100

● From 25 to 50
# Studied areas

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$$N_{\text{total}} = \textbf{56 plots}$$

## 2 valleys

Vallée d'Aure
Pays de Sault

~190km distance

## 3 dieback level conditions

- **Healthy** (20 plots)

- **Low dieback level** (12 plots)

- **High dieback level** (24 plots)

## 2 salvage logging conditions
within **high dieback level**

- **Not harvested** (12 plots)

- **Harvested** (12 plots)
# Characteristics of each plot

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**1 Malaise trap** in the center of each plot

Sampled **4 times** (each month) from May to September

## Micro Dendro-habitats

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Larrieu et al., 2018

## Canopy openness

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$$N_{\text{total}} = \textcolor{red}{224} \text{ samples}$$

$$n_{\text{ecological variables}} = \textcolor{red}{84+}$$
Sample preparation for metabarcoding

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* Processed sample randomization

Bálint et al., 2018

* Filtration & Rincing

* Drying overnight

* Homogeneization

Elbrecht et al., 2017

* Grinding
# Metabarcoding pipeline

* **COI** marker (313bp)

Leray et al., 2013

Geller et al., 2013

* PCR triplicates

* Illumina MiSeq V3 paired-end

* OTU clustering 97% (sumaclust)

* 3 PCRs & 3 reads minimum

* Taxonomic assignment using **BOLD**

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High general temporal turnover

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3041 OTUs

11.9% shared by all

August

1263 OTUS

Jaccard Index : $J_{May / August}$ = 0.23

Jaccard Index : $J_{June / July}$ = 0.43
Incomplete reference libraries
& taxonomic assignment

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**2977 OTUs**
when taxonomic
redundancies removed
Insect composition
varies with dieback level

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* **Strong difference** in insect composition between both valleys

* **Highly significant difference (***)** between **healthy** & **high level of dieback**

* **Low level of dieback** shows **no difference** in insect composition

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# Insect composition does not change with salvage logging

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* **Strong regional effect**

* **No significant difference** between **not harvested** and **salvage logged**

* **No direct impact of salvage logging** on total insect composition detected

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# Species richness does not vary with dieback level

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* No valley difference

* No significant difference between **Healthy**, **Low** and **High** dieback levels in terms of species richness detected

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# Species associated with dieback levels

## Potential candidates for bio-indication

### Healthy

12 specific OTUs

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CBG Photography Group - CC

### Fannia abrupta

Native from Nearctic
Apparently introduced in China (2016)
No information about France

### Low dieback level

21 specific OTUs

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### Pungeleria capreolaria

Feed on Picea abies and Abies alba
Fly from mid June to September

### High dieback level

10 specific OTUs

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Marko Mutanen - CC

### Botanophila brunneilinea

Larval stage on Centaurea sp. plants

Leuchtmann et al., 2015

### Package R Indicspecies

OTUs found at least in 10 plots
To sum up before flying away!

* Usefulness of **metabarcoding on Malaise** trap samples for **bio-monitoring**

* Strong **temporal** and **regional** effects

* Need to complete reference libraries for European **Diptera** and **Hymenoptera** in BOLD

* Direct impact of **high dieback level** on insect composition

* No direct impact of salvage logging

* Potential **bio-indicator candidates** for **dieback levels**

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Thank you for your attention !

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AGENCE NATIONALE DE LA RECHERCHE
ANR

IGB

Leibniz-Institut für
Gewässerökologie und Binnenfischerei

Thank you to:

* Kévin Speder

* Joël Meunier

* Cai Wang

* Yang Chunyan

* Susan Mbedi

* Laurent Burnel

* Jérôme Molina

* Carl Moliard

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BELMONT
F O R U M

ECOLE DOCTORALE SSBCV

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BeGenDiv

Berlin Center
for Genomics
in Biodiversity
Research

DAAD

Deutscher Akademischer Austauschdienst
German Academic Exchange Service

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CLIMTREE
Twin-tagging – Dual Indexing PCRs

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