# Supplement 2003–2007 (No. 47) to the White-Kauffmann-Le Minor scheme

Martine Guibourdenche, Peter Roggentin, Matthew Mikoleit, Patricia Fields, Jochen Bockemühl, Patrick A. D. Grimont, François-Xavier Weill

► To cite this version:

Martine Guibourdenche, Peter Roggentin, Matthew Mikoleit, Patricia Fields, Jochen Bockemühl, et al. Supplement 2003–2007 (No. 47) to the White-Kauffmann-Le Minor scheme. Research in Microbiology, 2010, 161 (1), pp.26-29. 10.1016/j.resmic.2009.10.002. pasteur-02019334

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Supplement 2003-2007 (no. 47) to the White-Kauffmann-Le Minor scheme

Martine Guibourdencheª, Peter Roggentinᵇ, Matthew Mikoleitᶜ, Patricia I.

Fieldsᶜ, Jochen Bockemühlᵇ, Patrick A.D. Grimontaª, François-Xavier Weilla*

ª Institut Pasteur, WHO Collaborating Centre for Reference and Research on *Salmonella*,
Unité de Biodiversité des Bactéries Pathogènes Emergentes, Paris, France

ᵇ Salmonella-Zentrale, Institut für Hygiene und Umwelt, Hamburg, Germany

ᶜ Centers for Disease Control and Prevention, Atlanta, Georgia, USA

* Corresponding author

WHO Collaborating Centre for Reference and Research on *Salmonella*, Laboratoire des
Bactéries Pathogènes Entériques, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris cedex
15, France. Tel: 33-(0)1 45 68 83 45. Fax: 33-(0)1 45 68 88 37. Email: fxweill@pasteur.fr
1 Abstract

2 This supplement reports the characterization of 70 new *Salmonella* serovars recognized

3 between 2003 and 2007 by the WHO Collaborating Centre for Reference and Research on

4 *Salmonella*: 44 were assigned to *Salmonella enterica* subspecies *enterica*, 11 to subspecies

5 *salamae*, 5 to subspecies *arizonae*, 8 to subspecies *diarizonae*, one to subspecies *houtenae*

6 and one to *S. bongori*. One new serovar, Mygdal, displayed a new H factor, H:z<sub>91</sub>.

7

8 Key words: *Salmonella*; Serovars; Taxonomy; White-Kauffmann-Le Minor scheme

9

10
# 1. Introduction

The genus *Salmonella* consists of only two species, *Salmonella enterica* and *S. bongori*.

*S. enterica* is divided into six subspecies: *S. enterica* subsp. *enterica*, *S. enterica* subsp. *salamae*, *S. enterica* subsp. *arizonae*, *S. enterica* subsp. *diarizonae*, *S. enterica* subsp. *houtenae*, and *S. enterica* subsp. *indica*; and.

This nomenclature reflects present understanding of *Salmonella* taxonomy [1, 4]. Serovars belonging to *S. enterica* subsp. *enterica* are typically designated by a name usually related to the geographical place where the serovar was first isolated. The serovar name is written in non-italicized Roman letters and the first letter capitalized. Serovars belonging to other subspecies are designated by their antigenic formulae, following the subspecies name. The antigenic formulae of *Salmonella* serovars are listed in a document called the White-Kauffmann-Le Minor scheme [3]. Updating this scheme is the responsibility of the WHO Collaborating Centre for Reference and Research on *Salmonella* (WHO-Salm), Institut Pasteur, Paris, France. The current edition (9th) issued in 2007 comprises antigenic formulae validated as of January 1st 2007.

Supplement 47 reports the characterization of 70 new *Salmonella* serovars recognized between 2003 and 2007 by the WHO-Salm: 44 were assigned to *S. enterica* subsp. *enterica*, 11 to subspecies *salamae*, 5 to subsp. *arizonae*, 8 to subsp. *diarizonae*, one to subspecies *houtenae*, and one to *S. bongori* (Table 1). Variants of previously described serovars are provided in Table 2. The present number of serovars per species and subspecies is given in
1 Table 3.

2 The strain 1357K (strain 9744/07) (Table 1), originally described by Kauffmann as the
3 reference strain of S. enterica subsp. enterica serovar Hisingen (48:a:1,5,7), was recently
4 found to be S. enterica subsp. salamae serovar 48:z<sub>81</sub>:1,5,7. Consequently the serovar
5 Hisingen will be withdrawn in the next issue of the White-Kauffmann-Le Minor scheme.

6 Additionally, a new H factor, H:Z<sub>91</sub>, was identified in one new serovar, S. enterica serovar
7 Mygdal (strain 9398/03) (Table 1). A partial sequence (1250 bp) of the fliC gene encoding
8 H:Z<sub>91</sub> has been submitted to GenBank (accession number GQ280905). The fliC allele
9 encoding H:Z<sub>91</sub> was related to alleles encoding H:b. Antiserum to H:Z<sub>91</sub> was prepared
10 following WHO-Salm guidelines [2]. Briefly, rabbits were immunized using strain 9398/03.
11 The crude antiserum (diluted 1:5) was successively absorbed with a boiled culture of strain
12 9398/03 to remove O agglutinins. Absorption with formalin-treated cultures of serovar Wien
13 strain 320K (H:b) and serovar Ohio strain CDC 1115-874/64 (H:b) were then performed to
14 remove cross-reactions with the H:b antigen. This absorbed antiserum agglutinated only the
15 motile culture of strain 9398/03.

Acknowledgements

18 We thank C. Fayolle (Institut Pasteur), S. Plavsic (Institut für Hygiene and Umwelt), P.
19 Dunker (Institut für Hygiene and Umwelt), L. Gheesling (CDC), S. Simington (CDC), and
1 M.S. Van Duyne (CDC) for their technical assistance.

2

3 We thank all the National Reference Centres that have sent the new serovars or variants to the WHO-Salm:

5

6 Austria :

7 Institut fur Medizinische Mikrobiologie (IMED), Graz (C. Kornschober)

8 Belgium :

9 -Institut Scientifique de Santé Publique, Brussels (S. Bertrand and J.M. Collard).

10

11 Canada :

12 -National Microbiology Laboratory (NML), Winnipeg (D. Woodward and H. Tabor).

13

14 -Laboratory for Foodborne Zoonoses (LFZ), Guelph (A. Muckle).

15 Denmark :

16 -Statens Serum Institute (SSI), Copenhagen (J. Sonne-Hansen).

17 Finland :

18 -National Public Health Institute (NPHI), Helsinki (S. Lukinmaa and A. Siitonen).

19

20 France :

21 -Institut Pasteur (IP), Paris (F.-X. Weill and P.A.D. Grimont).

22 Germany :

23 -Federal Institute for Risk Assessment (BfR), Berlin (A. Schroeter and C. Dorn).

24

25 -Institut für Hygiene and Umwelt (IHU), Hamburg (J. Bockemühl and P. Roggentin).

26

27 -Robert Koch Institute (RKI), Wernigerode (W. Rabsch).

28 Ireland :
1 -National University of Ireland (NUI), Galway (N. De Lappe and M.

2 Cormican).

3 **Israel :**

4 -Government Central Laboratories (GCL), Jerusalem (A. Reisfeld)

5 **New Zealand :**

6 -Institute of Environmental Science and Research Limited (ESR), Porirua (D.

7 Duncan).

8 **Norway :**

9 -Norwegian Institute of Public Health (NIPH), Oslo (J. Lassen)

10

11 **Senegal:**

12 -Institut Pasteur de Dakar (IPD), Dakar (J.-D. Perrier-Gros-Claude and B.

13 Garin).

14 **Sweden :**

15 -Swedish Institute for Infectious Disease Control (SIIDC), Solna (R. Wollin).

16 **Switzerland :**

17 -Institut de Bactériologie Vétérinaire (IBV), Bern (H. Hächler)

18 **Thailand :**

19 -National Institute of Health (TNIH), Nonthaburi (P. Sawanpanyalert).

20 **USA**

21 -Centers for Disease Control and Prevention (CDC), Atlanta (P.I. Fields)

22

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26
References

2 [1] Judicial Commission, The type species of the genus *salmonella* Lignieres 1900 is
3 *Salmonella enterica* (ex Kauffmann and Edwards 1952) Le Minor and Popoff 1987, with
4 the type strain LT2T, and conservation of the epithet *enterica* in *Salmonella enterica*
5 over all earlier epithets that may be applied to this species. Opinion 80, Int. J. Syst. Evol.
6 Microbiol. 55 (2005) 519-520.

7 [2] Popoff M.Y., Guidelines for the preparation of *Salmonella* antisera, 6th revision, WHO
8 Collaborating Centre for Reference and Research on *Salmonella*, Institut Pasteur, Paris,
9 2001.

10 [3] Grimont P.A.D, Weill F.-X., Antigenic formulae of the *Salmonella* serovars, 9th edition,
11 WHO Collaborating Centre for Reference and Research on *Salmonella*, Institut Pasteur,
12 Paris, 2007. Available at :
13 http://www.pasteur.fr/sante/clre/cadrecnr/salmoms/WKLM_En.pdf

14 [4] Tindall B.J., Grimont P.A.D., Garrity G.M., Euzeby J.P. Nomenclature and taxonomy of
15 the genus *Salmonella*, Int. J. Syst. Evol. Microbiol. 55 (2005) 521-524.

16
17
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1 **Table 1.** New *Salmonella* serovars recognized by the WHO Collaborating Centre for

2 Reference and Research on *Salmonella*, 2003-2007.

3

<table><thead><tr><th>Serovar name</th><th>Antigenic formula</th><th>Other characters<sup>c</sup></th><th>Source</th><th>Area of contamination or isolation</th><th>Year</th><th>IP strain number</th><th>Received from<sup>d</sup></th></tr></thead><tbody><tr><td colspan="8"><strong>Salmonella enterica subsp. enterica</strong></td></tr><tr><td>Korkeasaari</td><td>28:e,h:1,5</td><td></td><td>Lizard</td><td>Africa</td><td>2003</td><td>9370/03</td><td>NPHI</td></tr><tr><td>Moabit</td><td>16:e,h:l,w</td><td></td><td>Melon kernel Meal</td><td>Germany</td><td>2003</td><td>9374/03</td><td>BfR</td></tr><tr><td>Kaevlinge</td><td>16:z<sub>4</sub>,z<sub>24</sub>:-</td><td></td><td>Environment</td><td>Sweden</td><td>2002</td><td>9375/03</td><td>SIIDC</td></tr><tr><td>Mygdal</td><td>4,12:z<sub>91</sub>:-</td><td></td><td>Swine faeces</td><td>Denmark</td><td>2003</td><td>9398/03</td><td>SSI</td></tr><tr><td>Epalinges</td><td>43:l,w:-</td><td>01-</td><td>Environment</td><td>Switzerland</td><td>2003</td><td>9428/03</td><td>IBV</td></tr><tr><td>Farmingdale</td><td>43:z<sub>4</sub>,z<sub>23</sub>:-</td><td></td><td>Lizard</td><td>USA</td><td>2003</td><td>9447/03</td><td>CDC</td></tr><tr><td>Amberg</td><td>6,14,24:l,v:1,7</td><td></td><td>Caraway seed</td><td>Germany</td><td>2003</td><td>9455/03</td><td>BfR</td></tr><tr><td>Sally</td><td>41:z:1,6</td><td></td><td>Beef meat</td><td>Senegal</td><td>2003</td><td>9460/03</td><td>IP/IPD</td></tr><tr><td>Evry</td><td>35:i:z<sub>6</sub></td><td>Tar-</td><td>Human stools</td><td>France</td><td>2003</td><td>9462/03</td><td>IP</td></tr><tr><td>Albertbanjul</td><td>44:r:1,5</td><td>01-</td><td>Meat</td><td>Senegal</td><td>2003</td><td>9475/04</td><td>IP/IPD</td></tr><tr><td>Martonos</td><td>6,14,24:d:1,5</td><td></td><td>Parsley</td><td>Serbia</td><td>2004</td><td>9485/04</td><td>IHU</td></tr><tr><td>Gueuletapee</td><td>9,12:g,m,s:-</td><td></td><td>Chicken</td><td>Senegal</td><td>2004</td><td>9489/04</td><td>IP</td></tr><tr><td>Lamphun</td><td>6,8:y:1,2</td><td></td><td>Animal feed</td><td>Thailand</td><td>2003</td><td>9491/04</td><td>TNIH</td></tr><tr><td>Parakou</td><td>1,42:l,w:z<sub>35</sub></td><td></td><td>Water</td><td>Africa</td><td>2004</td><td>9495/04</td><td>IHU</td></tr><tr><td>Hohentwiel</td><td>30:z:e,n,x,z<sub>15</sub></td><td></td><td>Paprika powder</td><td>Germany</td><td>2004</td><td>9497/04</td><td>IHU</td></tr><tr><td>Carpentras</td><td>38:z<sub>35</sub>:e,n,z<sub>15</sub></td><td></td><td>Seed</td><td>France</td><td>2004</td><td>9500/04</td><td>IP</td></tr><tr><td>Lund</td><td>6,8:l,v:z<sub>6</sub></td><td></td><td>Human stools</td><td>Sweden</td><td>2003</td><td>9502/04</td><td>SIIDC</td></tr><tr><td>Willamette</td><td>38:d:1,5</td><td>01-</td><td>Human stools</td><td>USA</td><td>2004</td><td>9508/04</td><td>CDC</td></tr><tr><td>Yellowknife</td><td>9,12:r:e,n,x</td><td></td><td>Human</td><td>Canada</td><td>2004</td><td>9523/04</td><td>NML</td></tr><tr><td>Marsabit</td><td>52:l,w:1,5</td><td>01-</td><td>Camel</td><td>Kenya</td><td>2004</td><td>9527/04</td><td>BfR</td></tr><tr><td>Dingiri</td><td>17:z:1,6</td><td></td><td>Human blood</td><td>Gambia</td><td>2004</td><td>9529/04</td><td>SSI</td></tr><tr><td>Myrria</td><td>13,23:i:1,7</td><td></td><td>Human stools</td><td>Nigeria</td><td>2004</td><td>9544/05</td><td>NIPH</td></tr><tr><td>Eaubonne</td><td>18:g,s,t:-</td><td></td><td>Human stools</td><td>France</td><td>2004</td><td>9552/05</td><td>IP</td></tr><tr><td>Namur</td><td>39:z<sub>4</sub>,z<sub>23</sub>:-</td><td>01-</td><td>Human stools</td><td>Belgium</td><td>2005</td><td>9585/05</td><td>IP/IPD</td></tr><tr><td>Lonestar</td><td>41:c:-</td><td>01-</td><td>Human stools</td><td>USA</td><td>2005</td><td>9592/05</td><td>CDC</td></tr><tr><td>Winslow</td><td>13,22:z:1,5</td><td>Gal+</td><td>Wild pig</td><td>USA</td><td>2005</td><td>9593/05</td><td>CDC</td></tr><tr><td>Salinas</td><td>40:a:1,7</td><td></td><td>Human stools</td><td>USA</td><td>2005</td><td>9596/05</td><td>CDC</td></tr><tr><td>Picpus</td><td>13,23:z<sub>35</sub>:1,6</td><td></td><td>Human stools</td><td>France</td><td>2005</td><td>9597/05</td><td>IP</td></tr><tr><td>Ivrysurseine</td><td>13,23:z:z<sub>6</sub></td><td></td><td>Human stools</td><td>France</td><td>2005</td><td>9598/05</td><td>IP</td></tr><tr><td>Chennai</td><td>4,12:d:z<sub>35</sub>:-</td><td></td><td>Prawns</td><td>New Zealand</td><td>2005</td><td>9627/06</td><td>ESR</td></tr><tr><td>Sandaga</td><td>3,10:z<sub>38</sub>:1,2</td><td></td><td>Human stools</td><td>Senegal</td><td>2006</td><td>9647/06</td><td>IP/IPD</td></tr><tr><td>Umbadah</td><td>1,3,19:d:1,2</td><td></td><td>Bovine</td><td>Sudan</td><td>2006</td><td>9663/06</td><td>LFZ</td></tr><tr><td>Heistopdenberg</td><td>8,20:b:l,w</td><td></td><td>Human</td><td>Belgium</td><td>2006</td><td>9665/06</td><td>IP/IPD</td></tr><tr><td>Etobicoke<sup>a</sup></td><td>28:d:z<sub>6</sub></td><td>01-</td><td>Human</td><td>Canada</td><td>2006</td><td>9677/07</td><td>NML</td></tr><tr><td>Penzing<sup>a</sup></td><td>35:k:e,n,z<sub>15</sub></td><td>01-</td><td>Human</td><td>Austria</td><td>2005</td><td>9697/07</td><td>IMED</td></tr><tr><td>Portedeslilas<sup>a</sup></td><td>1,42:l,v:1,6,7</td><td>Dul-</td><td>Human blood</td><td>France</td><td>2007</td><td>9700/07</td><td>IP</td></tr><tr><td>Neuland<sup>a</sup></td><td>16:z<sub>35</sub>:1,5</td><td></td><td>Plant environment</td><td>Germany</td><td>2007</td><td>9713/07</td><td>IHU</td></tr><tr><td>Boston<sup>a</sup></td><td>13,23:c:e,n,z<sub>15</sub></td><td></td><td>Human stools</td><td>USA</td><td>2007</td><td>9716/07</td><td>CDC</td></tr><tr><td>Leinster<sup>a</sup></td><td>45:b:1,6</td><td></td><td>Human</td><td>Nigeria</td><td>2000</td><td>9721/07</td><td>NUI</td></tr><tr><td>Galway<sup>a</sup></td><td>13,23:k:e,n,z<sub>15</sub></td><td></td><td>Human</td><td>Nigeria</td><td>2007</td><td>9722/07</td><td>NUI</td></tr><tr><td>Ulm<sup>a</sup></td><td>1,6,14,25:z<sub>10</sub>:z<sub>6</sub></td><td></td><td>Sesame seed</td><td>Germany</td><td>2007</td><td>9743/07</td><td>IHU</td></tr></tbody></table>
<table><tbody><tr><td>Darfur<sup>a</sup></td><td>43:z<sub>41</sub>:1,2</td><td>Human</td><td>Tchad</td><td>2007</td><td>9748/07</td><td>RKI</td></tr><tr><td>Miromesnil<sup>a</sup></td><td>1,3,19:z<sub>4</sub>,z<sub>23</sub>:-</td><td>Human stools</td><td>France</td><td>2007</td><td>9758/07</td><td>IP</td></tr><tr><td>Serenli<sup>a</sup></td><td>1,13,23:z:1,5</td><td>Human stools</td><td>Africa</td><td>2006</td><td>9759/07</td><td>NIPH</td></tr><tr><td colspan="7"><strong>subsp. salamae</strong></td></tr><tr><td>48:z<sub>39</sub>:z<sub>81</sub></td><td>Gel-, Sal+</td><td>Human stools</td><td>USA</td><td>2003</td><td>9505/04</td><td>CDC</td></tr><tr><td>6,7:z<sub>4</sub>,z<sub>23</sub>:-</td><td>01-,ONPG+</td><td>Human</td><td>Canada</td><td>2004</td><td>9521/04</td><td>NML</td></tr><tr><td>40:m,t:e,n,x</td><td>01-</td><td>Beef</td><td>Namibia</td><td>2002</td><td>9539/05</td><td>NIPH</td></tr><tr><td>40:l,z<sub>28</sub>:e,n,x</td><td></td><td>Beef</td><td>Namibia</td><td>2002</td><td>9540/05</td><td>NIPH</td></tr><tr><td>28:m,t:z<sub>39</sub></td><td></td><td>Meat</td><td>Namibia</td><td>2003</td><td>9543/05</td><td>NIPH</td></tr><tr><td>40:z<sub>10</sub>:e,n,x</td><td>ONPG+</td><td>Chameleon</td><td>Germany</td><td>2005</td><td>9583/05</td><td>BfR</td></tr><tr><td>17:d :-<sup>a</sup></td><td></td><td>Beef</td><td>Africa</td><td>2006</td><td>9669/06</td><td>NIPH</td></tr><tr><td>60:z<sub>10</sub>:z<sub>39</sub><sup>a</sup></td><td>Gel-</td><td>Human stools</td><td>USA</td><td>2007</td><td>9714/07</td><td>CDC</td></tr><tr><td>6,7:z<sub>38</sub>:e,n,x<sup>a</sup></td><td></td><td>Human stools</td><td>Mozambique</td><td>2007</td><td>9733/07</td><td>NPHI</td></tr><tr><td>48:z<sub>81</sub>:1,5,7<sup>a,b</sup></td><td>Gel-</td><td>Fish meal</td><td>Angola</td><td>1961</td><td>9744/07</td><td>IP/IHU</td></tr><tr><td>9,46:l,w:e,n,x<sup>a</sup></td><td></td><td>Beef</td><td>Namibia</td><td>2006</td><td>9760/07</td><td>NIPH</td></tr><tr><td colspan="7"><strong>subsp. arizonae</strong></td></tr><tr><td>48:z<sub>29</sub>:-</td><td></td><td>Human stools</td><td>USA</td><td>2002</td><td>9379/03</td><td>CDC</td></tr><tr><td>45:z<sub>36</sub>:-</td><td></td><td>Snake</td><td>USA</td><td>2003</td><td>9464/03</td><td>CDC</td></tr><tr><td>21:z<sub>36</sub>:-</td><td></td><td>Human stools</td><td>USA</td><td>2005</td><td>9595/05</td><td>CDC</td></tr><tr><td>50:g,z<sub>51</sub>:-</td><td>GGT+</td><td>Human stools</td><td>USA</td><td>2005</td><td>9628/06</td><td>CDC</td></tr><tr><td>18:z<sub>36</sub>:-<sup>a</sup></td><td></td><td>Human blood</td><td>USA</td><td>2006</td><td>9715/07</td><td>CDC</td></tr><tr><td colspan="7"><strong>subsp. diarizonae</strong></td></tr><tr><td>48:z<sub>4</sub>,z<sub>24</sub>:-</td><td>Muc+, Glu-</td><td>Snake</td><td>Switzerland</td><td>2002</td><td>9407/03</td><td>UVB</td></tr><tr><td>8:k:z<sub>35</sub></td><td>GGT-, Mal-</td><td>Avian</td><td>USA</td><td>2003</td><td>9446/03</td><td>CDC</td></tr><tr><td>52:k:e,n,x,z<sub>15</sub></td><td></td><td>Water</td><td>France</td><td>2003</td><td>9453/03</td><td>IP</td></tr><tr><td>63:(k):z</td><td></td><td>Snake</td><td>USA</td><td>2003</td><td>9488/04</td><td>CDC</td></tr><tr><td>43:g,t:-</td><td>Muc+, Glu-</td><td>Human stools</td><td>Sweden</td><td>2004</td><td>9504/04</td><td>SIIDC</td></tr><tr><td>8:r:z</td><td>Glu-, Gel-</td><td>Human stools</td><td>USA</td><td>2003</td><td>9507/04</td><td>CDC</td></tr><tr><td>50:i:z<sub>53</sub></td><td>Muc+, Tar+</td><td>Chameleon</td><td>Germany</td><td>2005</td><td>9604/05</td><td>BfR</td></tr><tr><td>65:i:z</td><td>Muc+</td><td>Reptile</td><td>Germany</td><td>2006</td><td>9648/06</td><td>BfR</td></tr><tr><td colspan="7"><strong>subsp. houtenae</strong></td></tr><tr><td>18:g,z<sub>51</sub>:-</td><td></td><td>Human</td><td>USA</td><td>2006</td><td>9654/06</td><td>CDC</td></tr><tr><td colspan="7"><strong>Salmonella bongori</strong></td></tr><tr><td>1,9,12:d:e,n,x<sup>a</sup></td><td></td><td>Cattle</td><td>Finland</td><td></td><td>9736/07</td><td>NPHI</td></tr></tbody></table>

<sup>a</sup>Serovar name or antigenic formula not recorded in the current (9th) edition of the White-Kauffmann-Le

Minor scheme.

<sup>b</sup>Corresponds to Kauffmann's strain 1357K, originally described as the reference strain of *S. enterica*

subsp. *enterica* serovar Hisingen (48:a:1,5,7).

<sup>c</sup>The differential phenotypical characters of *Salmonella* species and subspecies are indicated in

reference 3. Only the atypical results are mentioned. Tests used : β-galactosidase, ONPG ; β-

glucuronidase, GLU ; gelatinase, Gel ; galacturonate, Gal ; malonate, Mal ; salicin, Sal ; dulcitol,

Dul ; mucate, Muc ; L(+)-tartrate (= d-tartrate), Tar ; γ-glutamyltransferase, GGT ; lyse by phage O1,

O1. +, positive reaction ; -, negative reaction.

<sup>d</sup>The National Reference Centres having sent the isolates are indicated in the acknowledgments section.
1 **Table 2.** New variants of previously described *Salmonella* serovars recognized by the WHO Collaborating Centre for Reference and Research on

2 *Salmonella*, 2003-2007.

3

<table><thead><tr><th>IP strain number</th><th>Antigenic formula</th><th>Other characters<sup>a</sup></th><th>Source</th><th>Area of contamination or isolation</th><th>Year</th><th>Received from<sup>b</sup></th><th>Serovar name</th><th>Updated antigenic formula</th></tr></thead><tbody><tr><td colspan="9"><strong>S. enterica subsp. enterica</strong></td></tr><tr><td>9429/03</td><td>43:l,w:Z<sub>44</sub></td><td>01-</td><td>Human</td><td>Switzerland</td><td>2003</td><td>UVB</td><td>Epalinges</td><td>43:l,w:[Z<sub>44</sub>]</td></tr><tr><td>9445/03</td><td>4,12:g,m,s:1,2</td><td>Tar-</td><td>Turkey</td><td>USA</td><td>2003</td><td>CDC</td><td>Hato</td><td><u>1,4,[5],12:g,m,s:[1,2]</u></td></tr><tr><td>9450/03</td><td>{6,7,14}<br>{54}:g,m,s:-</td><td></td><td>Calf meat</td><td>Germany</td><td>2003</td><td>IHU</td><td>Montevideo</td><td>{6,7,<u>14</u>} {54}:g,m,[p],s:[1,2,7]</td></tr><tr><td>9467/03</td><td>1,4,12:i:e,n,Z<sub>15</sub></td><td></td><td>Human stools</td><td>France</td><td>2003</td><td>IP</td><td>Tsevie</td><td><u>1,4,12:i:e,n,Z<sub>15</sub></u></td></tr><tr><td>9549/05</td><td>43:Z<sub>4</sub>,Z<sub>23</sub>:1,2</td><td></td><td>Human stools</td><td>Norway</td><td>2004</td><td>NIPH</td><td>Farmingdale</td><td>43:Z<sub>4</sub>,Z<sub>23</sub>:[1,2]</td></tr><tr><td>9554/05</td><td>1,4,12:e,h:e,n,Z<sub>15</sub></td><td></td><td>Sunflower kernels</td><td>Germany</td><td>2004</td><td>IHU</td><td>Sandiego</td><td><u>1,4,[5],12:e,h:e,n,Z<sub>15</sub></u></td></tr><tr><td>9645/06</td><td>1,4,12:r:e,n,Z<sub>15</sub></td><td></td><td>Cattle</td><td>Sudan</td><td>2006</td><td>LFZ</td><td>Drogana</td><td><u>1,4,12,[27]:r,[i]:e,n,Z<sub>15</sub></u></td></tr><tr><td>9658/06</td><td>1,4,12:r:l,w</td><td></td><td>Human stools</td><td>France</td><td>2006</td><td>IP</td><td>Bochum</td><td><u>1,4,[5],12:r:l,w</u></td></tr><tr><td>9717/07</td><td>4,5,12:b:1,5</td><td>Muc-</td><td>Human stools</td><td>USA</td><td>2006</td><td>CDC</td><td>Limete</td><td><u>1,4,[5],12:b:1,5<sup>c</sup></u></td></tr><tr><td>9740/07</td><td>42:l,v:1,6,7</td><td>Dul-</td><td>Human stools</td><td>Finland</td><td>2007</td><td>NPHI</td><td>Portedeslilas</td><td><u>1,4:21,v:1,6,7<sup>c</sup></u></td></tr><tr><td>9742/07</td><td>1,13,23:d,l,</td><td></td><td>Human stools</td><td>France</td><td>2007</td><td>IP</td><td>Putten</td><td><u>1,13,23:d,l,w<sup>c</sup></u></td></tr><tr><td>9752/07</td><td>1,4,12,27:l,v:1,5<sup>a</sup></td><td></td><td>Human stools</td><td>France</td><td>2007</td><td>IP</td><td>Azteca</td><td><u>1,4,[5],12,[27]:l,v:1,5<sup>c</sup></u></td></tr><tr><td colspan="9"><strong>S. enterica subsp. salamae</strong></td></tr><tr><td>9383/03</td><td>4,5,12:b:-</td><td></td><td>Turtle faeces</td><td>Germany</td><td>2003</td><td>BfR</td><td></td><td>1,4,[5],12,[27]:b:[e,n,x]</td></tr><tr><td colspan="9"><strong>S. enterica subsp. diarizonae</strong></td></tr><tr><td>9469/03</td><td>1,40:l,v:Z<sub>53</sub></td><td></td><td>Human</td><td>Israel</td><td>2003</td><td>GCL</td><td></td><td><u>1,40:l,v:Z<sub>53</sub></u></td></tr><tr><td colspan="9"><strong>S. enterica subsp. indica</strong></td></tr><tr><td>9437/03</td><td>6,14:a:e,n,x</td><td>01-</td><td>Environment</td><td>Switzerland</td><td>2003</td><td>UVB</td><td></td><td>[1],6,14,[25]:a:e,n,x</td></tr></tbody></table>

4

5 <sup>a</sup>The differential phenotypical characters of *Salmonella* species and subspecies are indicated in reference 3. Only the atypical results are mentioned. Tests

6 used : dulcitol, Dul ; mucate, Muc ; L(+) tartrate (= d-tartrate), Tar; lyse by phage O1, O1. +, positive reaction ; -, negative reaction.

7 <sup>b</sup>The National Reference Centres having sent the isolates are indicated in the acknowledgments section.

8 <sup>c</sup>Antigenic formula not recorded or not updated in the current (9th) edition of the White-Kauffmann-Le Minor scheme.
<table><caption>Table 3. Present number of serovars in each species and subspecies of *Salmonella*.</caption><thead><tr><th></th><th></th><th></th></tr></thead><tbody><tr><td>1</td><td></td><td></td></tr><tr><td>2</td><td></td><td></td></tr><tr><td>3</td><td></td><td></td></tr><tr><td>4</td><td>S. enterica</td><td></td></tr><tr><td>5</td><td>subsp. enterica</td><td>1547</td></tr><tr><td>6</td><td>subsp. salamae</td><td>513</td></tr><tr><td>7</td><td>subsp. arizonae</td><td>100</td></tr><tr><td>8</td><td>subsp. diarizonae</td><td>341</td></tr><tr><td>9</td><td>subsp. houtenae</td><td>73</td></tr><tr><td>10</td><td>subsp. indica</td><td>13</td></tr><tr><td>11</td><td></td><td></td></tr><tr><td>12</td><td>S. bongori</td><td>23</td></tr><tr><td>13</td><td></td><td></td></tr><tr><td>14</td><td>Total</td><td>2 610</td></tr><tr><td>15</td><td></td><td></td></tr><tr><td>16</td><td></td><td></td></tr><tr><td>17</td><td></td><td></td></tr></tbody></table>