# Is Eriophyes mali Nalepa present in Italy?

Valeria Malagnini, Mario Baldessari, Federico Pedrazzoli, Alessia Tatti, Carlo

Duso, Enrico de Lillo, Gino Angeli, Mariusz Lewandowski

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Valeria Malagnini, Mario Baldessari, Federico Pedrazzoli, Alessia Tatti, Carlo Duso, et al. Is Eriophyes mali Nalepa present in Italy?. Acarologia, 2023, Acarologia, 63 (Suppl), pp.39-44. 10.24349/fgml-gv5c. hal-04171281

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# Is *Eriophyes mali* Nalepa present in Italy?

Valeria Malagnini<sup>a</sup>, Mario Baldessari<sup>a</sup>, Federico Pedrazzoli<sup>a</sup>, Alessia Tatti<sup>b,c</sup>, Carlo Duso<sup>d</sup>, Enrico de Lillo<sup>e</sup>, Gino Angeli<sup>a</sup>, Mariusz Lewandowski<sup>f</sup>

Proceedings of the 9th Symposium of the EurAAc, Bari, July, 12th-15th 2022

## ABSTRACT

In the last few years, blistering symptoms were observed on apple plants in commercial orchards. Blisters are commonly found on apple leaves as well as on small fruits. This symptom is compatible with that described for apple blister mites belonging to the genus *Eriophyes* (Eriophyidae). To assess the identity of the etiological agent, leaf blisters and buds of symptomatic apple and, as a control, pear plants were examined under the dissection microscope and eriophyoid mites were collected. Specimens were examined using both molecular and morphological approaches. The analysis of sequences confirmed that eriophyoid mites collected from symptomatic apple and pear plants are genetically different. Our analyses highlight a complex scenario inside the genus *Eriophyes* that is worth to be studied in more detail.

**Keywords** Eriophyoidea; apple; pear; DNA barcoding

## Introduction

Eriophyoid mites (Acari: Prostigmata: Eriophyoidea) are obligate plant feeders, and most of them exhibit high levels of host specificity and adaptability (Lindquist 1996; Skoracka and Dabert 2010; de Lillo et al. 2018). They inhabit all plant parts except roots and can cause economically important damage to their hosts (Oldfield 1996; Westphal and Manson 1996). More than a dozen of eriophyoid species have been reported on apple plants (Malus domestica Borkh.) all over the world (Vidović et al. 2014; Amrine and de Lillo, unpublished database). Among them, the apple rust mite *Aculus schlechtendali* (Nalepa, 1890), a vagrant mite causing damages with high population densities (Duso et al. 2010), is the most widespread species in Italy.

In the last few years, leaf blistering was widely observed on apple leaves in commercial orchards of Northern Italy. These symptoms were reported for the first time in 2017 in Val di Non, which is one of the most important apple-growing areas of Northern Italy, and in several orchards in the Emilia-Romagna region. These damages were not compatible with those associated with the apple rust mite but looked like the blister galls described for the pear

<figure><img src="image_7.png" /></figure>

<footnote><sup>a</sup>Fondazione Edmund Mach - Centro Trasferimento Tecnologico via E. Mach, 1 - 38010 San Michele all'Adige (TN) Italy.</footnote>

<footnote><sup>b</sup>Scuola Universitaria Superiore IUSS Pavia, Palazzo del Broletto, Piazza Vittoria 15, 27100 Pavia, Italy.</footnote>

<footnote><sup>c</sup>Center Agriculture Food Environment C3A, University of Trento and Fondazione Edmund Mach, Trento, Italy.</footnote>

<footnote><sup>d</sup>Department of Agronomy, Food, Natural resources, Animals and Environment, University of Padova Via dell'Università 16 - 35020 Legnaro (PD), Italy.</footnote>

<footnote><sup>e</sup>Dipartimento di Scienze del Suolo, della Pianta e degli Alimenti, University of Bari Aldo Moro, via Amendola, 165/a - 70126 Bari, Italy.</footnote>

<footnote><sup>f</sup>Department of Plant Protection, Institute of Horticultural Sciences, Warsaw University of Life Sciences, ul. Nowoursynowska 159 - 02776 Warsaw, Poland.</footnote>
blister mite *Eriophyes pyri* (Pagenstecher, 1857) and the apple blister mite *Eriophyes mali*
Nalepa, 1926.

The apple blister mite was described for the first time by Nalepa (1926) as *Eriophyes pyri*
var. *mali* and was considered for a long time as a variety of *E. pyri* because of the morphological
similarities and the production of identical symptoms on apple leaves (blister galls) (Vidović *et al.* 2014).
Later, Liro and Roivainen (1951) changed the mite’s taxonomic status and raised it
to the rank of species [*E. mali* (Nal.) Liro (nov. comb.)]. In the late 50s, Burts (1970) described
a new eriophyid species, *E. mali* Burts, 1970, which can be considered as a junior synonym
of *E. mali* Nal. (Vidović *et al.* 2014), on apple samples collected in Washington (Pacific
Northwestern USA). So far, *E. mali* has not been recorded in Italy, while the presence of *E. pyri*
is quite common and well-documented for a long time (Canestrini 1890; Vidano *et al.* 1978).

In the present work, we carried out a molecular analysis to assess the identity of eriophyoid
mites causing blisters on apple leaves and recently found in Northern Italy. As diagnostic
structures for morphological analysis are not always enough for species discrimination and this
often causes the misidentification of species (de Lillo et al. 2010), DNA-based approaches are
often adopted to overcome this problem (Navajas and Navia 2010), like in the current case of
eriophyoid mites collected on apple vs pear plants.

**Material and methods**

Eriophyoid mite specimens were collected from leaf blisters and buds of symptomatic apple and pear plants in the Trentino-Alto Adige region. Apple plants in three different orchards located in Trentino - Val di Non (Rumo, 46.433975°N and 11.028116°E, and Coredo, 46.356663°N and 11.083217°E) and Alto Adige (Vadena, 46.379604°N and 11.287893°E) and some pear plants in Alto Adige (Salorno, 46.241207°N and 11.205038°E) were sampled in 2020 and 2021. Symptomatic leaves and buds were observed under a dissecting microscope, and single individuals were collected using a brush with only one bristle and placed in a 1.5 ml Eppendorf tube.

The DNA from single individuals was isolated using 5% Chelex® 100 (Bio-Rad) (Walsh *et al.* 2013). Each sample, after adding 10 µl of a solution of Chelex and proteinase K (95: 5), was incubated at 50 °C for 1h and then at 95 °C for 10 min. Five individuals were analyzed for each population.

The cytochrome oxidase subunit I (COX1) gene was amplified with the primer pair LCO1490 and HCO2198 (Folmer et al. 1994). Polymerase chain reaction (PCR) conditions were as reported in EPPO (2021). PCR products, after purification with illustra ExoProStar1-Step (GE Healthcare, Little Chalfont, UK), were sequenced with the BigDye Terminator v3.1 cycle sequencing kit (Applied Biosystems, Foster City, CA, USA) on an Applied Biosystems 3130 xl Genetic Analyzer (Carlsbad, CA, USA). Sequences were manually checked, and BIOEDIT software (Hall 1999) was used for corrections and alignments. Sequences obtained were deposited in the GenBank (NCBI) (accession numbers from OP593091 to OP593101 for samples from apple; OP593102 and OP593103 for samples from pear), and a search was conducted using BLAST (Basic Local Alignment Search Tool). Then, we first built a dataset with a customed Python script retrieving 2,977 Eriophyidae COX1 sequences from Genbank. After aligning the sequences using MAFFT version 7.475 (Katoh and Standley 2013) with the “adjust direction accurately” option and cleaning the alignment with a customed Python script, an alignment with 662 positions was obtained. We analyzed a Maximum Likelihood (ML) exploratory tree with all 2,977 sequences and selected the clade that contained our newly sequenced species, removing the sequences that could cause a long branch attraction problem. The reduced dataset for the Eriophyidae family contained 304 sequences. We further trimmed the alignment obtaining a new alignment with 657 positions, and built the final ML phylogenetic tree, using IQ-TREE 1.6.12 (Nguyen et al. 2015) with the “model selection” option, 10,000 bootstrap replicates, and *A. schlechtendali* used as outgroup.
<figure><img src="image_8.png" /><figcaption>**Figure 1** Blisters in the undersurface of an apple leaf. The arrows indicate the entry circular opening of the galls.</figcaption></figure>

**Results and discussion**

**Biological observations**

Early symptoms of the eriophyid mite presence, not ascribable to *A. schlechtendali*, were observed at the beginning of the spring. In this period, the upper surface of the leaves shows yellowish spots, while blisters, as well as the entry circular opening, are more visible in the undersurface (Fig. 1). Later in the season, blisters become larger and turn brownish, and the large under-epidermic cavities contain eriophyoids at different stages. These symptoms are similar to those described for apple blister mites (Vidović et al. 2014). Galls can also occur on the superficial layers of small fruits (Fig. 2). During the winter, eriophyoid mites can be found under the bud scales (Fig. 3).

**Genetic data**

The phylogenetic analysis indicates that eriophyoid mites collected from apple plants in different orchards of Trentino-Alto Adige belong to the same species and that mites collected from apple are different from those collected from pear plants. The identity score obtained blasting the COX1 sequences of apple mites against sequences of pear mites suggests even the possible presence of different genera (e.g., the GenBank identity score for OP593091 vs OP593102 is 79.97%).

This seems in contradiction with the preliminary morphological analyses (in verbis de Lillo) of samples collected from apple and pear plants. In fact, the typical diagnostic characteristics of the genus *Eriophyes* were observed in both mites, suggesting that they belong to the same genus.

These contradictory data highlight a very complex scenario inside the genus *Eriophyes*. For this reason, further molecular analyses, involving other molecular markers combined with morphological and ecological observations, are needed to better understand the identity of these samples.

The maximum likelihood tree calculated with IQ-TREE is shown in Fig. 4, where clades phylogenetically distant from the sequences of our samples are collapsed. The sequences submitted to GenBank as *Eriophyes* spp. are scattered across the phylogenetic tree and do
<figure><img src="image_9.png" /><figcaption>**Figure 2** Blisters on the surface of apple fruits in May. Arrows indicate some of the blisters present in the small fruits.</figcaption></figure>

not form a monophyletic group. According to this phylogenetic tree, the sister species of *E. pyri* from pear, with 100% bootstrap, is *Eriophyes calycobius* (Nalepa 1891) from *Crataegus monogyna* Jacq. (Rosaceae) (the GenBank identity score between OP593102 and MW691980 is 84.02%). Regarding apple eriophyoids, the sister species is *E. pyri* (accession number EU254715.1), with 100% bootstrap. This sequence is 444 bp long, and the low GenBank identity score with apple eriophyoids suggests different species (e.g. the identity score between EU254715.1 and OP593091.1 is 84.20%).

<figure><img src="image_10.png" /><figcaption>**Figure 3** Eriophyoid mites (average length ± s.e.: 182.4±55 µm) under the bud scales during the winter.</figcaption></figure>
<figure><img src="image_11.png" /><figcaption>**Figure 4** Maximum likelihood tree for the Eriophyidae family calculated with IQ-TREE. The best fit model chosen according to BIC is TIM+F+I+G4. The bootstrap values are shown at each node. Sequences of the samples collected from pear and apple trees are highlighted in red, while the sequences reported in GenBank as belonging to species in the genus *Eriophyes* are in green. The outgroup, *A. schlechtendali*, is in blue.</figcaption></figure>

The species *E. mali* has been recorded in North America, New Zealand, the European part of Russia, and many European countries (Vidović *et al.* 2014), but so far not in Italy. If the eriophyoid samples collected on apple plants in the Trentino district were confirmed to be *E. mali*, this would be the first record for Italy.

**Acknowledgements**

The authors thank Manfred Wolf (Laimburg Research Centre) for collecting and providing mite samples collected on apple plants from Alto Adige.

**ORCID**

Valeria Malagnini ID https://orcid.org/0000-0001-9686-0299

Mario Baldessari ID https://orcid.org/0000-0003-4687-9404

Federico Pedrazzoli ID https://orcid.org/0000-0002-3841-4650

Alessia Tatti ID https://orcid.org/0000-0001-9628-528X

Carlo Duso ID https://orcid.org/0000-0002-2600-2536

Enrico de Lillo ID https://orcid.org/0000-0003-0364-4963

Gino Angeli ID https://orcid.org/0000-0002-4924-0481

Mariusz Lewandowski ID https://orcid.org/0000-0001-5360-0382
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