# High-Performance Liquid Chromatography Assay for Moxifloxacin in Brain Tissue and Plasma: Validation in a Pharmacokinetic Study in a Murine Model of Cerebral Listeriosis

Renaud Respaud, Solene Grayo, Eric Singlas, Sophie Dubouch, Alban Le

Monnier, Marie-Catherine Lott

► To cite this version:

Renaud Respaud, Solene Grayo, Eric Singlas, Sophie Dubouch, Alban Le Monnier, et al. High-Performance Liquid Chromatography Assay for Moxifloxacin in Brain Tissue and Plasma: Validation in a Pharmacokinetic Study in a Murine Model of Cerebral Listeriosis. Journal of Analytical Methods in Chemistry, 2012, 2012, pp.1-7. 10.1155/2012/436349. hal-05463794

HAL Id: hal-05463794

https://hal.science/hal-05463794v1

Submitted on 20 Jan 2026

HAL is a multi-disciplinary open access
archive for the deposit and dissemination of sci-
entific research documents, whether they are pub-
lished or not. The documents may come from
teaching and research institutions in France or
abroad, or from public or private research centers.

L'archive ouverte pluridisciplinaire HAL, est
destinée au dépôt et à la diffusion de documents
scientifiques de niveau recherche, publiés ou non,
émanant des établissements d'enseignement et de
recherche français ou étrangers, des laboratoires
publics ou privés.

<figure><img src="image_1.png" /></figure>

Distributed under a Creative Commons CC BY 4.0 - Attribution - International License
<figure><img src="image_2.png" /></figure>

Research Article

# High-Performance Liquid Chromatography Assay for Moxifloxacin in Brain Tissue and Plasma: Validation in a Pharmacokinetic Study in a Murine Model of Cerebral Listeriosis

Renaud Respaud,<fnref n="1" /> Solene Grayo,<fnref n="2" /> Eric Singlas,<fnref n="1" /> Sophie Dubouch,<fnref n="1" /> Alban Le Monnier,<fnref n="2" /> and Marie-Catherine Lott<fnref n="1" />

Correspondence should be addressed to Renaud Respaud, respaudrenaud@yahoo.fr

Received 21 November 2011; Accepted 29 December 2011

Academic Editor: Adam Voelkel

Copyright © 2012 Renaud Respaud et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Moxifloxacin is a broad-spectrum antibacterial 8-methoxy-fluoroquinolone. In order to evaluate the pharmacokinetic properties of moxifloxacin in mouse plasma and brain tissue, we developed a high-performance liquid chromatography (HPLC) method. This study was based on single-drug delivery, intravenously dosed in a central listeriosis murine model. The method employed a reversed-phase Lichrospher RP-18 with a precolumn (250 × 4.6 mm) and a mobile phase composed of a mixture of acetonitrile, methanol, and citric buffer (pH = 3.5) with sodium dodecyl sulfate and tetrabutylammonium bromide. Fluorescence detection was performed at an excitation wavelength of 290 nm and an emission wavelength of 550 nm. The relative standard deviation of intra- and inter-day assays was <10%. This validated method led to a short retention time (8.0 min) for moxifloxacin. The standard curves were linear from 5–250 µg/L in plasma and from 0.1–2.5 µg/g of brain tissue. The limits of quantification were 5 µg/L in plasma and 0.1 µg/g in brain tissue. The method enabled the detection of systemic antimicrobial in plasma and in CNS in *Listeria*-infected mice. Injected moxifloxacin passed through the encephalic barrier within a 30 to 60 min after injection time frame. Moxifloxacin pharmacokinetics are modeled in an infected model compared to control mice.

## 1. Introduction

Moxifloxacin (1-cyclopropyl-6-fluoro-1,4-dihydro-8-methoxy-7-[(4aS.7aS)-octahydro-6H-pyrrolo-[3,4-b]pyridin-6-yl]-4-oxo-3-quinolinecarboxylic acid hydrochloride, BAY 12-8039) is a fluoroquinolone molecule with a broad antibacterial spectrum of activity encompassing gram-negative and gram-positive bacteria [1, 2].

*Listeria monocytogenes* is a gram-positive bacterium that is widespread in the environment [3]. It is a facultative intracellular food-borne pathogen that causes severe and life-threatening infections, especially septicemia, abortions, and central nervous system (CNS) infections [3]. Listeriosis mainly occurs in high-risk groups, including individuals

with severe underlying diseases or with impaired immunity [4]. Importantly, moxifloxacin has rapid bactericidal activity against extracellular and intracellular *L. monocytogenes* [5]; consequently, this drug can be considered more effective than amoxicillin, the reference treatment, which is only bacteriostatic against *L. monocytogenes* [6]. Moreover, Grayo et al. determined minimal inhibitory concentrations (MICs) for a large collection of *L. monocytogenes* strains and did not detect any that were resistant, regardless of their origin [5]. Secondly, they confirmed that moxifloxacin has rapid *in vivo* activity against *L. monocytogenes* in BALB/c mice [7]. To assess the efficiency of moxifloxacin in this murine model of central listeriosis [7], a pharmacokinetic study of this fluoroquinolone in plasma and brain was necessary.

<footnote n="1">¹Laboratoire de Suivi Thérapeutique et de Contrôle des Médicaments, Service de Pharmacie, Assistance Publique-Hôpitaux de Paris (AP-HP), Hôpital Necker-Enfants Malades, 75015 Paris, France</footnote>

<footnote n="2">²Laboratoire de Listeria, Centre National de Référence des Listeria and World Health Organization Collaborating Centre for Foodborne Listeriosis, Institut Pasteur, 75015 Paris, France</footnote>
<figure><img src="image_3.png" /><figcaption>FIGURE 1: Chemical structures of the fluoroquinolones: (a) moxifloxacin (1-cyclopropyl-6-fluoro-1,4-dihydro-8-methoxy-7-[(4aS,7aS)-octahydro-6H-pyrrolo-[3,4-b]pyridin-6-yl]-4-oxo-3-quinolinecarboxylic acid hydrochloride, BAY 12-8039); (b) ciprofloxacin (internal standard).</figcaption></figure>

Indeed, some results can even be misleading: fosfomycin has recently been declared effective against *L. monocytogenes* in vivo, whereas it is not in vitro [8].

In plasma, moxifloxacin quantification methods have been performed to clarify its clinical efficacy [9–12]. Due to the intracellular development of *L. monocytogenes*, it seems more relevant to measure moxifloxacin concentrations in CNS tissue rather than in cerebrospinal fluid (CSF) [13, 14]. However, no bioanalytical assay has been performed to determine moxifloxacin concentrations in brain tissue (CNS). Thus, we developed a high-performance liquid chromatographic (HPLC) method for the determination of moxifloxacin in plasma and brain. Our method was then utilised to evaluate the pharmacokinetic parameters of moxifloxacin in plasma and brain in a validated murine model of CNS listeriosis.

## 2. Experimental

### 2.1. Chemicals and Reagents.
Moxifloxacin (Figure 1(a)) and ciprofloxacin (Figure 1(b)) were purchased from Bayer Pharma (Germany and France). Prolabo (Fontenay-sous-Bois/France) provided analytical-grade (Normapur) dipo-tassium hydrogenphosphate, potassium dihydrogenphosphate and orthophosphoric acid (85%). Sigma (Saint Quentin Fallavier, France) provided the tetrabutylammonium bromide (TBABr). Graded acetonitrile and methanol were purchased from Prolabo.

Stock solutions of moxifloxacin HCl and ciprofloxacin were in methanol (1 g/L): working standard solutions and working internal solutions were in methanol (3 mg/L).

Citric buffer consisted of 25 mM citric acid, 10 mM sodium dodecyl sulfate (SDS), and 10 mM TBABr in 500 mL water; the pH was adjusted to 3.5 with 0.1 M NaOH.

### 2.2. Instrumentation.
Chromatographic analysis was performed on a HPLC system consisting of a pump (LC 10A, Shimadzu, Touzart et Matignon, France), thermostat (maintained at 25°C) 717 Plus autosampler Waters, and a RF 551 fluorescence detector (Shimadzu, Touzart et Matignon, France). Separation was executed by a reversed-phase Lichrospher C18 precolumn and column (250 × 4 mm, 5 µm) (Merck, Darmstadt, Germany). All data were analyzed using

Chromleon (version 6.7). The mobile phase consisted of an acetonitrile-methanol-buffer mixture (pH 3.5) (40:3:57, v/v/v) at a flow rate of 1.0 mL/min. Fluorescence detection was performed at an excitation wavelength of 290 nm and an emission wavelength of 550 nm.

### 2.3. Sample Preparation.
To 100 µL of plasma or brain (0.5 g crushed in 0.5 mL of water), 20 µL of the working internal standard solution was added. The samples were deproteinized with acetonitrile (100 µL). After centrifugation (3000 rpm for 10 minutes), the supernatant (100 µL) was mixed with sterile water (400 µL); finally, 20 µL of this solution was injected into the chromatographic system.

For the brain, as described above, we added the ciprofloxacin solution (20 µL) to 100 µL of crushed brain. Then, repeating the plasma sample process, 20 µL of the solution was injected into the chromatographic system.

### 2.4. Method Validation.
Validation experiments were designed according to the “Guidance for Industry-bioanalytical Method Validation,” recommended by the US Food and Drug Administration (FDA) [15, 16]. The specificity of the method was evaluated by assaying mouse blank plasma (n = 48) and mouse brain tissue (n = 6) from separate animals. As validation of the method was performed in human plasma and experiments performed in mouse plasma, composition of control mice, and human plasma samples was previously compared.

The assay was considered satisfactory if precision, expressed as relative standard deviation (RSD) or coefficient of variation (%CV), was less than 15% for within- and between-run variation. Quality control samples were independently prepared from the calibration standards, according to Section 2.4. The samples contained three different concentrations of moxifloxacin: near the lower limit of quantification (LOQ), mid-point of the concentration range and near the upper LOQ. Two replicates of each control sample were processed and analyzed over three validation days. The assay precision has been calculated using the RSD and a one-way analysis of variance. The RSD for the lower LOQ was set at <20%.

Linearity was assessed by plotting calibration curves in human plasma and duplicated in three separate runs.
Calibration was done by spiking different moxifloxacin concentrations in plasma and CNS tissue with solutions: between 5 and 250 µg/L (5, 10, 50, 75, 100, 150, 175, 200, and 250 µg/L) and between 0.1 and 2.5 µg/g of brain (0.1, 0.5, 0.75, 1.0, 1.5, 1.75, 2.0, and 2.5 µg/g of brain). The curves were fitted by a linear regression method through measurement of the peak area ratio of moxifloxacin into the internal standard solution.

Recovery of moxifloxacin was determined by comparing the postextraction quality control samples mean peak area ratio to the mean of the methanol standards (n = 9). Recovery was determined in three different runs at three concentrations (quality controls).

Moxifloxacin stability was acquired by analyzing replicates (n = 3) at three different concentrations (quality control samples). The following stability conditions were evaluated for each method: long-term stability at -20°C, -80°C, three freeze-thaw cycles, and 18 h after sample treatment in the autosampler. An interval of ±15% on either side of the initial concentration was applied to assess analyte stability.

## 2.5. Pharmacokinetic Application

### 2.5.1. Experimental Models.
BALB/c female mice, 7-8 weeks old, purchased from Elevage Janvier (Le Genest-St- Isle, France) were used. The virulent strain *L. monocytogenes* EGDe [17] was provided by the bacteriology laboratory of the Pasteur Hospital (Paris, France) and used to induce listeriosis in these animals.

Mice were weighed (23 to 27 g), then injected intravenously via the lateral tail vein with $1 \times 10^5 L. monocytogenes$ in 0.5 mL of saline isotonic solution to induce listeriosis. At 36 hours after infection, mice were treated with an intraperitoneal (i.p.) injection of moxifloxacin (50 mg/kg, 0.1 mL). This protocol was approved by the Animal Welfare Committee of the Pasteur Institute.

### 2.5.2. Sample Collection.
The pharmacokinetic profiles of moxifloxacin in mouse plasma and brain tissue (infected or not) were analyzed immediately after a single dose of moxifloxacin. Moxifloxacin concentrations were measured from samples collected at 0, 5, 15, 30, 60, 120, 240, 360, and 480 minutes after a single-drug injection. At each sampling time, 6 animals per group were sacrificed.

Brains were washed in sterile saline to clear the circulating blood, then dried, weighed (0.5 g ± 0.01 g), crushed, and homogenized aseptically and centrifuged. Plasma was obtained from blood by centrifugation. Cerebral supernatants and plasma samples were frozen immediately and stored at -80°C until analysis.

### 2.5.3. Pharmacokinetic Analysis.
Concentration time data were analyzed using a noncompartmental model with zero-order absorption and first-order elimination via a nonlinear least squares technique. Pharmacokinetic parameters were estimated through standard methods. Maximal plasma and brain concentrations ($C_{max}$) were determined directly by

<figure><img src="image_4.png" /><figcaption>FIGURE 2: Chromatogram of moxifloxacin (retention time = 8.0 min) and the internal standard ciprofloxacin (retention time = 6.0 min) in plasma (concentration = 250 µg/L).</figcaption></figure>

inspection of the experimental concentration time curves, the values for the area under the concentration time curve (AUC_t) were calculated by using the trapezoidal rule until the last concentration was measured. Penetration rate (AUC brain/AUC plasma) and the ratio sizing pharmacokinetic parameter variations between control and infected mice were also calculated. Results are expressed as means ± standard deviation.

## 3. Results

### 3.1. Validation of the Method.
Following the assay conditions described above, moxifloxacin was separated from the internal standard, ciprofloxacin; retention times, were 6.0 and 8.0 minutes, respectively (Figure 2). The chromatograms obtained from the analysis of two blank matrices show no interfering peaks with the same retention times and no interfering peak was observed for the retention times of the two fluoroquinolones from human plasma, mouse plasma, and mouse brain. The resolution between ciprofloxacin and moxifloxacin was up to 2.

The calibration curve obtained by plotting peak: area ratio (moxifloxacin/internal standard) versus concentration was linear over the range 5 to 250 µg/L in plasma and 0.1 to 2.5 µg/g in cerebral tissue. Linearity was observed in our moxifloxacin analyses from plasma and brain tissue ($r^2$ = 0.999 for both) over the evaluated concentration ranges. The plasma quantification lower limit was 5.0 µg/L and 0.1 µg/g for brain, matching the calibration curves first point. Excellent results were obtained for precision (CV < 10%; CV < 5%) and accuracy (99.2–111.5%; 94.8–106.2%) for plasma and brain tissue, respectively (n = 6 for each control; Tables 1(a) and 1(b)).

The mean moxifloxacin extraction recovery from plasma and cerebral tissue was above 75% (data not shown). The stability experiment was carried out under four conditions. Triplicates of each quality control sample were analyzed. Specimens stored at -20°C and at -80°C were stable for at least 4 months (data not shown). Moxifloxacin remained stable over three freeze-thaw cycles (between-run variation
<table><caption>TABLE 1: Reproducibility of moxifloxacin plasma concentration (a) and brain tissue concentration (b) measurements: assay performance data (n = 6).</caption><thead><tr><th colspan="8" style="text-align: center;">(a)</th></tr><tr><th rowspan="2">Nominal concentration (µg/L)</th><th rowspan="2">Measured concentration (µg/L)</th><th colspan="2">Coefficient of variation (%)</th><th rowspan="2">Measured concentration (µg/L)</th><th colspan="2">Coefficient of variation (%)</th></tr><tr><th>Within-run</th><th>Accuracy (%)</th><th>Between-run</th><th>Accuracy (%)</th></tr></thead><tbody><tr><td>20</td><td>22.3 ± 0.3</td><td>1.5</td><td>111.5</td><td>19.9 ± 1.9</td><td>9.5</td><td>99.0</td></tr><tr><td>125</td><td>125.5 ± 1.3</td><td>1.0</td><td>100.4</td><td>125.6 ± 2.8</td><td>2.2</td><td>100.5</td></tr><tr><td>225</td><td>240.4 ± 3.6</td><td>1.5</td><td>106.8</td><td>230.9 ± 8.1</td><td>3.5</td><td>102.6</td></tr></tbody></table><table><thead><tr><th colspan="8" style="text-align: center;">(b)</th></tr><tr><th rowspan="2">Nominal concentration (µg/g)</th><th rowspan="2">Measured concentration (µg/g)</th><th colspan="2">Coefficient of variation (%)</th><th rowspan="2">Measured concentration (µg/g)</th><th colspan="2">Coefficient of variation (%)</th></tr><tr><th>Within-run</th><th>Accuracy (%)</th><th>Between-run</th><th>Accuracy (%)</th></tr></thead><tbody><tr><td>0.2</td><td>0.19 ± 0.01</td><td>3.6</td><td>94.8</td><td>0.21 ± 0.01</td><td>5.0</td><td>105.5</td></tr><tr><td>1.25</td><td>1.33 ± 0.03</td><td>2.5</td><td>106.2</td><td>1.30 ± 0.04</td><td>3.0</td><td>104.3</td></tr><tr><td>2.25</td><td>2.17 ± 0.03</td><td>1.4</td><td>96.6</td><td>2.26 ± 0.11</td><td>5.0</td><td>100.4</td></tr></tbody></table>

<table><caption>TABLE 2: Plasma and cerebral pharmacokinetic parameters after intraperitoneal administration of moxifloxacin (50 mg/kg) in infected and control mice (n = 6).</caption><thead><tr><th></th><th>T<sub>max</sub> (h)</th><th>C<sub>max</sub><br>(mg/L)</th><th>T<sub>1/2</sub> (h)</th><th>Cl (L/h)</th><th>Vd (L/kg)</th><th>AUC<sub>0-24h</sub><br>(mg·h/L)</th></tr></thead><tbody><tr><td>Plasma</td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>Infected</td><td>0.75</td><td>17.3 ± 6.6</td><td>1.9</td><td>0.02</td><td>3.5</td><td>27.1</td></tr><tr><td>Control</td><td>1</td><td>6.7 ± 1.2</td><td>1.1</td><td>0.08</td><td>6.5</td><td>9.5</td></tr><tr><td>Brain tissue</td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>Infected</td><td>0.75</td><td>2.0 ± 0.2</td><td>21.7</td><td></td><td></td><td>3.3</td></tr><tr><td>Control</td><td>1</td><td>0.8 ± 0.3</td><td>10.7</td><td></td><td></td><td>1.0</td></tr></tbody></table>

rate <11% for plasma and <10% for brain, and recovery ranged from 99% to 103% for plasma and from 100% to 105% for brain). Moxifloxacin also remained stable 18 h after sample treatment (CV < 5%, recovery between 98% to 105% for plasma and 99% to 106% for brain).

### 3.2. Pharmacokinetic Study.
The methods described above were utilised in the determination of mean moxifloxacin concentrations from plasma and the CNS after a single i.p. injection (50 mg/kg) in *L. monocytogenes* infected and control mice. The pharmacokinetic profiles are represented in Figures 3(a) and 3(b), and the parameters determined for moxifloxacin are shown in Table 2.

In plasma, for infected mice, the moxifloxacin $C_{max}$ was reached at 0.75 h ($C_{max}$ 17.3 ± 6.6 mg/L) versus 1 h ($C_{max}$ 6.7 ± 1.2 mg/L) for the control group. Nevertheless, after 1 hour, the concentration of moxifloxacin rapidly dropped and became undetectable after 8 hours (versus 6 hours for the control group). The plasma $AUC_{24}$ was 27.1 mg·h/L and 9.5 mg·h/L in infected and control mice, respectively. The $AUC_{24}$/MIC ratio (AUIC) for moxifloxacin was 54, and the $C_{max}$/MIC ratio was 34 in infected mice.

In cerebral tissue, moxifloxacin was quickly detected (5 minutes) and its concentration peaked at 2.0 ± 0.2 µg/g

(compared with 0.8 ± 0.3 µg/g in the control group) within 1 hour of i.p. administration. After 0.75 h, the cerebral concentration of moxifloxacin decreased, but was still detectable 8 hours after administration (versus 6 hours in controls).

The cerebral $AUC_{24}$ was 3.3 µg·h/g and 1.0 µg·h/g in infected and control mice, respectively. The $AUC_{brain}$/ $AUC_{plasma}$ ratio was always greater than 0.1.

## 4. Discussion

In this study, we described a HPLC method to quantify moxifloxacin penetration in plasma and brain. Optimization of the method of Ba et al. was performed by modifying several preanalytical and analytical steps [9]. In particular, we assessed and fine-tuned the pH and composition of the mobile phase, SDS concentration, addition of TBABr, and detection mode. Mouse plasma and brain samples were processed by deproteinization with acetonitrile due to the low-protein binding of moxifloxacin [1]. Due to the extraction efficiency, we decided to validate the method using ciprofloxacin as an internal standard.

Separation was achieved by ion-pairing reversed-phase chromatography with a 10 mM concentration of SDS [11]. Liang et al. showed that above this concentration,
<figure><img src="image_5.png" /><figcaption>FIGURE 3: (a) Plasma concentration time course (mg/L) of moxifloxacin in mice (infected ■ or not ▲) after a single i.v. administration of 50 mg per kg of bodyweight (bolus injection) (n = 6); (b) brain concentration time course (µg/g of brain) of moxifloxacin in mice (infected ■ or not ▲) after a single i.v. administration of 50 mg per kg of body weight (bolus injection) (n = 6).</figcaption></figure>

fluoroquinolone resolution was not improved and column equilibration time was optimum. Competing-base agent (TBABr) was added at the concentration of 10 mM to increase resolution between moxifloxacin and ciprofloxacin, and to improve the peak shape [9, 11]. The pH of the mobile phase was fixed at 3.5 with citric acid buffer at a concentration of 25 mM [11]. Finally, the optimized mobile phase consisted of 10 mM TBABr, 10 mM SDS, 25 mM citric acid, 40% acetonitrile, and 3% methanol at pH 3.5. Due to the properties of moxifloxacin and ciprofloxacin, fluorescence detection was used because of its better sensitivity and specificity than UV detection in complex matrix for pharmacokinetic studies.

The HPLC method based on standard chromatographic conditions, rapid sample preparation and analysis (less than 10 min) has been developed for a dual-matrix moxifloxacin determination (plasma and cerebral tissue). The lower LOQ was found to be 5 µg/L in plasma and 0.1 µg/g in brain. The LOQ in plasma is similar to that reported in recent studies [10, 18]. Moreover, we did not need derivatization with a fluorescent molecule [19]. In plasma and brain tissue, it is low enough to enable pharmacokinetic studies in mice, other animals, or humans. This HPLC method is helpful and essential to study the pharmacokinetics and cerebral penetration of moxifloxacin. Several methods have been described for moxifloxacin quantification in different matrices (plasma, urine, cerebrospinal fluid, peritoneum, lung, etc.) [11, 14, 20]. However, most of these methods are described for only one matrix and some of them have very high LOQ (90 µg/L for the method described by Hemanth Kumar and Ramachandran) [18] which are not suitable for pharmacokinetic studies in mice.

To our knowledge, this study is the first to evaluate moxifloxacin brain penetration by measuring concentrations in a murine model of cerebral listeriosis after i.p. treatment. Rodriguez-Cerrato et al. determined moxifloxacin CSF penetration in a rabbit model of *Escherichia coli* meningitis [14]. Kanellakopoulou et al. showed the pharmacokinetics of moxifloxacin in noninflamed CSF of humans [13, 14]. These two studies investigated moxifloxacin pharmacokinetics in

CSF. In contrast, we have investigated moxifloxacin pharmacokinetics in plasma and brain tissue due to the intracellular development of *L. monocytogenes*. Subsequently, we have shown modification of moxifloxacin pharmacokinetics between infected or control mice (plasma and brain data).

Moxifloxacin brain penetration was shown according to its presence in CSF, as highlighted by Rise et al. [21]. Indeed, plasma and CNS tissue kinetics increased by 2.9 and 3.3 times, respectively, between control and infected mice ($C_{max}$, half-life, $AUC_{0-24h}$). Inflammation of the brain-blood barrier could explain the increase of these parameters in brain but not in plasma. The increase in plasma could be due to a diminution of hepatic metabolism or renal elimination due to infection by *L. monocytogenes*.

Our study is not without limitations including the omission of weighing animals before and after infection with *L. monocytogenes* to determine potential dehydration, which could account for the increase in plasma concentrations of moxifloxacin in infected compared with control mice. Also, we did not quantitate the concentration of moxifloxacin in the CSF of mice to distinguish between diffusion in the blood-brain barrier and diffusion in brain tissue. Finally, we did not determine the concentration of moxifloxacin in urine; a reduction in renal elimination of moxifloxacin in infected mice might explain the increased serum moxifloxacin concentration in infected animals.

As Grayo et al. showed, AUIC ($AUC_{plasma}$/MIC) is the most relevant parameter to demonstrate *in vivo* efficiency. For fluoroquinolones, an AUIC value that appears sufficient *in vitro* and in animal and clinical trials, and for other infections, is between 30 [22] and 125 [23]; in our study it was equal to 54 and could explain the efficacy of moxifloxacin in CNS listeriosis [7]. In contrast to Alffenaar et al., we assessed cerebral penetration by determination of the $AUC_{brain}/AUC_{plasma}$ ratio, and not the $AUC_{CSF}/AUC_{plasma}$ ratio, which is the more relevant quantitative marker of antibacterial diffusion in tissue [24]. These ratios were always greater than 0.1, varying from plasma to brain. Even if the $AUC_{brain}/AUC_{plasma}$ ratio is not high, the AUIC in brain is enough to explain the *in vivo* efficacy of moxifloxacin [7].
Subsequently, we showed diffusion of moxifloxacin (5 minutes after injection) in cerebral tissue in infected mice unlike amoxicillin, considered in [6]. This rapid diffusion is probably related to increased blood-brain barrier permeability due to the inflammation of the meninges resulting in prolonged detection of the drug in the brain tissue [7, 21, 25]. Moreover, moxifloxacin can be detected 8 hours after administration in infected mice and 6 hours in control mice. This confirms the good level of brain penetration of moxifloxacin as demonstrated by Wise et al. [21] and it could explain the *in vivo* efficiency [7].

## 5. Conclusion

We have developed and validated a HPLC moxifloxacin determination method in plasma and brain tissue in a mouse model. Based on this sensitive method, we demonstrated moxifloxacin penetration in the CNS for the first time in this listeriosis model. As expected, we suggest that infection can modulate the pharmacokinetic profile of moxifloxacin. Indeed, the higher antimicrobial concentrations can be correlated with improved diffusion into infected tissue (plasma and cerebral) in listeriosis than in a healthy model. This could explain the systemic antimicrobial *in vivo* efficiency of moxifloxacin against *L. monocytogenes*. Further, clinical data are required to confirm these findings.

Due to an excellent separation efficiency, sensitivity by fluorescent detection and its simplicity, this HPLC method could potentially meet the analysis requirements for any other complex tissues (such as liver or lung), or for therapeutic drug monitoring of moxifloxacin in other human diseases (such as cholecystitis or tuberculosis).

## Conflict of Interests

The authors declare that they have no conflict of interests.

## Acknowledgments

The authors thank Nila Bhana of inScience Communications, a Wolters Kluwer business, who provided assistance with English language editing. This assistance was funded by Astellas, France.

## References

[1] J. A. B. Balfour and L. R. Wiseman, "Moxifloxacin," *Drugs*, vol. 57, no. 3, pp. 363-374, 1999.

[2] "The Merck index," p. 1125, 2001.

[3] J. M. Farber and P. I. Peterkin, "Listeria monocytogenes, a food-borne pathogen," *Microbiological Reviews*, vol. 55, no. 3, pp. 476-511, 1991.

[4] V. Goulet and P. Marchetti, "Listeriosis in 225 non-pregnant patients in 1992: clinical aspects and outcome in relation to predisposing conditions," *Scandinavian Journal of Infectious Diseases*, vol. 28, no. 4, pp. 367-374, 1996.

[5] S. Grayo, O. Join-Lambert, M. C. Desroches, and A. Le Monnier, "Comparison of the in vitro efficacies of moxifloxacin and amoxicillin against Listeria monocytogenes,"

*Antimicrobial Agents and Chemotherapy*, vol. 52, no. 5, pp. 1697-1702, 2008.

[6] H. Hof, "An update on the medical management of listeriosis," *Expert Opinion on Pharmacotherapy*, vol. 5, no. 8, pp. 1727-1735, 2004.

[7] S. Grayo, M. C. Lott-Desroches, O. Dussurget et al., "Rapid eradication of Listeria monocytogenes by moxifloxacin in a murine model of central nervous system listeriosis," *Antimicrobial Agents and Chemotherapy*, vol. 52, no. 9, pp. 3210-3215, 2008.

[8] M. Scortti, L. Lacharme-Lora, M. Wagner, I. Chico-Calero, P. Losito, and J. A. Vázquez-Boland, "Coexpression of virulence and fosfomycin susceptibility in Listeria: molecular basis of an antimicrobial in vitro-in vivo paradox," *Nature Medicine*, vol. 12, no. 5, pp. 515-517, 2006.

[9] B. B. Ba, R. Etienne, D. Ducint, C. Quentin, and M. C. Saux, "Determination of moxifloxacin in growth media by high-performance liquid chromatography," *Journal of Chromatography B*, vol. 754, no. 1, pp. 107-112, 2001.

[10] A. Laban-Djurdjević, M. Jelikić-Stankov, and P. Djurdjević, "Optimization and validation of the direct HPLC method for the determination of moxifloxacin in plasma," *Journal of Chromatography B*, vol. 844, no. 1, pp. 104-111, 2006.

[11] H. Liang, M. B. Kays, and K. M. Sowinski, "Separation of levofloxacin, ciprofloxacin, gatifloxacin, moxifloxacin, trovafloxacin and cinoxacin by high-performance liquid chromatography: application to levofloxacin determination in human plasma," *Journal of Chromatography B*, vol. 772, no. 1, pp. 53-63, 2002.

[12] H. M. Siefert, A. Domdey-Bette, K. Henninger, F. Hucke, C. Kohlsdorfer, and H. H. Stass, "Pharmacokinetics of the 8-methoxyquinolone, moxifloxacin: a comparison in humans and other mammalian species," *Journal of Antimicrobial Chemotherapy*, vol. 43, supplement B, pp. 69-76, 1999.

[13] K. Kanellakopoulou, A. Pagoulatou, K. Stroumpoulis et al., "Pharmacokinetics of moxifloxacin in non-inflamed cerebrospinal fluid of humans: implication for a bactericidal effect," *Journal of Antimicrobial Chemotherapy*, vol. 61, no. 6, pp. 1328-1331, 2008.

[14] V. Rodriguez-Cerrato, C. C. McCoig, I. C. Michelow et al., "Pharmacodynamics and bactericidal activity of moxifloxacin in experimental Escherichia coli meningitis," *Antimicrobial Agents and Chemotherapy*, vol. 45, no. 11, pp. 3092-3097, 2001.

[15] ICH, "Validation of analytical procedures: methodology. Guideline 2QB," *Federal Register*, vol. 62, no. 96, pp. 27463-27467, 1997.

[16] ICH, "Note for guidance on validation of analytical methods: definitions and terminology (Q2A)," in *Proceedings of the International Conference of Harmonisation*, 1994.

[17] P. Glaser, L. Frangeul, C. Buchrieser et al., "Comparative genomics of Listeria species," *Science*, vol. 294, no. 5543, pp. 849-852, 2001.

[18] A. K. Hemanth Kumar and G. Ramachandran, "Simple and rapid liquid chromatography method for determination of moxifloxacin in plasma," *Journal of Chromatography B*, vol. 877, no. 11-12, pp. 1205-1208, 2009.

[19] S. Tatar Ulu, "High-performance liquid chromatography assay for moxifloxacin: pharmacokinetics in human plasma," *Journal of Pharmaceutical and Biomedical Analysis*, vol. 43, no. 1, pp. 320-324, 2007.

[20] C. Østergaard, T. K. Sørensen, J. D. Knudsen, and N. Frimodt-Møller, "Evaluation of moxifloxacin, a new 8-methoxyquinolone, for treatment of meningitis caused by a
penicillin-resistant pneumococcus in rabbits,” *Antimicrobial Agents and Chemotherapy*, vol. 42, no. 7, pp. 1706–1712, 1998.

[21] R. Wise, J. M. Andrews, G. Marshall, and G. Hartman, "Pharmacokinetics and inflammatory-fluid penetration of moxifloxacin following oral or intravenous administration," *Antimicrobial Agents and Chemotherapy*, vol. 43, no. 6, pp. 1508–1510, 1999.

[22] M. B. Kays and G. A. Denys, "Fluoroquinolone susceptibility, resistance, and pharmacodynamics versus clinical isolates of Streptococcus pneumoniae from Indiana," *Diagnostic Microbiology and Infectious Disease*, vol. 40, no. 4, pp. 193–198, 2001.

[23] M. J. Montgomery, P. M. Beringer, A. Aminimanizani et al., "Population pharmacokinetics and use of Monte Carlo simulation to evaluate currently recommended dosing regimens of ciprofloxacin in adult patients with cystic fibrosis," *Antimicrobial Agents and Chemotherapy*, vol. 45, no. 12, pp. 3468–3473, 2001.

[24] J. W. C. Alffenaar, R. Van Altena, H. J. Bökkerink et al., "Pharmacokinetics of moxifloxacin in cerebrospinal fluid and plasma in patients with tuberculous meningitis," *Clinical Infectious Diseases*, vol. 49, no. 7, pp. 1080–1082, 2009.

[25] J. D. Chaudhuri, "Blood brain barrier and infection," *Medical Science Monitor*, vol. 6, no. 6, pp. 1213–1222, 2000.