# Methane emissions in sheep infected with parasitic nematodes and supplemented with Acacia mearnsii

Paulo de Mello Tavares Lima, Pierre Crouzoulon, Geneviève Zabré, Adama Kaboré, Vincent Niderkorn, Herve Hoste, Adibe Luiz Abdalla, Helder Louvandin

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Paulo de Mello Tavares Lima, Pierre Crouzoulon, Geneviève Zabré, Adama Kaboré, Vincent Niderkorn, et al.. Methane emissions in sheep infected with parasitic nematodes and supplemented with Acacia mearnsii. 7. GGAA Greenhouse Gases & Animal Agriculture Conference (GGAA), 2019, Iguassu Falls. hal-02735207

HAL Id: hal-02735207

https://hal.inrae.fr/hal-02735207

Submitted on 2 Jun 2020

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# Methane emissions in sheep infected with parasitic nematodes and supplemented with Acacia mearnsii

Conference Paper · August 2019

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Paulo de Mello Tavares Lima

University of São Paulo

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Adama Kaboré

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Vincent Niderkorn

French National Institute for Agricultural Research

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Hervé Hoste

French National Institute for Agricultural Research

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Some of the authors of this publication are also working on these related projects:

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Productive aspects and methane emission in Santa Inês sheep supplemented with the legume Macrotyloma axillare View project

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index of quality of government institutions View project
7th GGAA – Greenhouse Gas and Animal Agriculture Conference
August 4th to 8th, Iguassu Falls/Brazil

Proceedings

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# Methane emissions in sheep infected with parasitic nematodes and supplemented with *Acacia mearnsii*

Paulo de Mello Tavares Lima<fnref n="1" />, Pierre Crouzoulon<fnref n="2" />, Geneviève Zabré<fnref n="3" />, Adama Kabore<fnref n="4" />, Vincent Niderkorn<fnref n="5" />, Hervé Hoste<fnref n="6" />, Adibe Luiz Abdalla<fnref n="7" /> and Helder Louvandini⁸

Our objective was to evaluate the effects of *Acacia mearnsii* bark extract (PAB) on methane (CH₄) production in sheep infected with Trichostrongylus colubriformis and Haemonchus contortus. Twenty 10 month-old Santa Inês lambs were used in a 50-day trial. Four treatment groups were formed: two control groups uninfected, one without PAB (C-) (n=4) and one with PAB (C+) (n=4); and two infected groups, one without PAB (I-) (n=6) and another receiving PAB (I+) (n= 6). Animals were kept in individual pens for 40 days, and received ad libitum chopped tifton 85 hay (Cynodon spp.) and 210 g/animal/day of concentrate (PAB supplementation: 15 g/animal/day – in concentrate). Animals were reared indoors to avoid helminthic infections (faecal egg count (FEC) was monthly controlled). At day 0, infected groups were artificially infected with L3 larvae of T. colubriformis (7500) and H. contortus (5000). After 28 days post-infection, establishment of infections was confirmed by FEC: I-: 1150 + 530.1 and I+: 567 + 544.6 eggs/gram of feces. After 40 days of trial (during which other parameters were evaluated - data not shown), CH₄ emissions in chambers were measured and variance analysis (proc GLM, SAS®) showed no PAB effects on methanogenesis (p > 0.05), however, both infected groups had higher CH₄ emissions than the control groups: C-: 16.7 + 10.91; C+:23.7 + 12.88; I-: 43.1 + 6.37; I+: 40.6 + 6.02 (g CH₄/kg dry matter intake) (p < 0.05). The methanogenic effect of parasitic infections should be explored in studies addressing sustainability of animal production.

Keywords: Black wattle, Haemonchus contortus, Small chamber, Trichostrongylus colubriformis, Tannins

<footnote n="1">¹Universidade de São Paulo, Centro de Energia Nuclear na Agricultura, Piracicaba, São Paulo, Brasil – PhD - Post-Doctoral Researcher</footnote>

<footnote n="2">²Universidade de São Paulo, Centro de Energia Nuclear na Agricultura, Piracicaba, São Paulo, Brasil – MSc Student</footnote>

<footnote n="3">³Laboratoire de Biologie et Santé Animales-DPA/INERA, Ouagadougou, Burkina Faso MSc - PhD Student</footnote>

<footnote n="4">⁴Laboratoire de Biologie et Santé Animales-DPA/INERA, Ouagadougou, Burkina Faso PhD - Professor</footnote>

<footnote n="5">⁵Université Clermont Auvergne, INRA, VetAgro Sup, UMR Herbivores, F-63122 Saint-Genès Champanelle, France – PhD – Scientific Researcher</footnote>

<footnote n="6">⁶INRA, UMR, Chemin des Capelles, Toulouse, France – PhD – Senior Researcher</footnote>

<footnote n="7">⁷,⁸Universidade de São Paulo, Centro de Energia Nuclear na Agricultura, Piracicaba, São Paulo, Brasil - PhD - Associate Professor</footnote>