# Inhibition of jasmonate-mediated plant defences by the fungal metabolite higginsianin B

Jean-Felix Dallery, Marlene Zimmer, Vivek Halder, Mohamed Suliman, Sandrine Pigné, Géraldine Le Goff, Despoina D Gianniou, Ioannis P Trougakos, Jamal Ouazzani, Debora Gasperini, et al.

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## To cite this version:

Jean-Felix Dallery, Marlene Zimmer, Vivek Halder, Mohamed Suliman, Sandrine Pigné, et al. Inhibition of jasmonate-mediated plant defences by the fungal metabolite higginsianin B. Journal of Experimental Botany, 2020, 71 (10), pp.2910-2921. 10.1093/jxb/eraa061. hal-02722418

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RESEARCH PAPER

# Inhibition of jasmonate-mediated plant defences by the fungal metabolite higginsianin B

Jean-Félix Dallery<fnref n="1" />,<fnref n="4" />, ID, Marlene Zimmer<fnref n="2" />, Vivek Halder<fnref n="3" />,*, ID, Mohamed Suliman<fnref n="3" />,†, Sandrine Pigné<fnref n="1" />, Géraldine Le Goff<fnref n="4" />, Despoina D. Gianniou<fnref n="5" />, Ioannis P. Trougakos<fnref n="5" />, ID, Jamal Ouazzani<fnref n="4" />, Debora Gasperini<fnref n="2" /> and Richard J. O'Connell<fnref n="1" />,‡, ID

Received 4 November 2019; Editorial decision 22 January 2020; Accepted 29 January 2020

Editor: Robert Hancock, The James Hutton Institute, UK

## Abstract

Infection of *Arabidopsis thaliana* by the ascomycete fungus *Colletotrichum higginsianum* is characterized by an early symptomless biotrophic phase followed by a destructive necrotrophic phase. The fungal genome contains 77 secondary metabolism-related biosynthetic gene clusters, whose expression during the infection process is tightly regulated. Deleting *CclA*, a chromatin regulator involved in the repression of some biosynthetic gene clusters through H3K4 trimethylation, allowed overproduction of three families of terpenoids and isolation of 12 different molecules. These natural products were tested in combination with methyl jasmonate, an elicitor of jasmonate responses, for their capacity to alter defence gene induction in *Arabidopsis*. Higginsianin B inhibited methyl jasmonate-triggered expression of the defence reporter *VSP1p:GUS*, suggesting it may block bioactive jasmonoyl isoleucine (JA-Ile) synthesis or signalling *in planta*. Using the JA-Ile sensor Jas9-VENUS, we found that higginsianin B, but not three other structurally related molecules, suppressed JA-Ile signalling by preventing the degradation of JAZ proteins, the repressors of jasmonate responses. Higginsianin B likely blocks the 26S proteasome-dependent degradation of JAZ proteins because it inhibited chymotrypsin- and caspase-like protease activities. The inhibition of target degradation by higginsianin B also extended to auxin signalling, as higginsianin B treatment reduced auxin-dependent expression of *DR5p:GUS*. Overall, our data indicate that specific fungal secondary metabolites can act similarly to protein effectors to subvert plant immune and developmental responses.

**Keywords:** *Colletotrichum*, fungal natural product, higginsianin, jasmonate signalling, JAZ protein, plant chemical biology, plant immunity, proteasome, secondary metabolite.

## Introduction

The perception of microbial plant aggressors is mediated by the recognition of pathogen-associated molecular patterns (PAMPs) by plant cell surface receptors, which in

turn activates a cascade of PAMP-triggered immune (PTI) responses (Dodds and Rathjen, 2010). Downstream of PTI activation, these immune responses are regulated by

<footnote n="1">¹ Université Paris-Saclay, INRAE, AgroParisTech, UMR BIOGER, Thiverval-Grignon, France</footnote>

<footnote n="2">² Department of Molecular Signal Processing, Leibniz Institute of Plant Biochemistry, Halle (Saale), Germany</footnote>

<footnote n="3">³ Chemical Biology Laboratory, Max Planck Institute for Plant Breeding Research, Cologne, Germany</footnote>

<footnote n="4">⁴ Centre National de la Recherche Scientifique, Institut de Chimie des Substances Naturelles ICSN, Gif-sur-Yvette, France</footnote>

<footnote n="5">⁵ Department of Cell Biology and Biophysics, Faculty of Biology, National and Kapodistrian University of Athens, Greece</footnote>

<footnote>* Current address: Rijk Zwaan, De Lier, 2678 ZG, Netherlands</footnote>

<footnote>† Current address: Desert Research Center, Cairo, Egypt</footnote>

<footnote>‡ Correspondence: richard.oconnell@inrae.fr or debora.gasperini@ipb-halle.de</footnote>
an interconnected network of phytohormone signalling pathways in which jasmonic acid (JA), ethylene, and salicylic acid (SA) play a central role (Pieterse et al., 2012). Antagonistic and synergistic interactions between these pathways provide an additional layer of regulation in which hormone cross-talk allows the plant to fine-tune its immune responses to particular pathogens (Pieterse et al., 2012; Bigeard et al., 2015). A broad range of microbes target these hormone signalling pathways, using secreted protein or small-molecule effectors in order to manipulate or circumvent plant immunity (Groll et al., 2008; Katsir et al., 2008; Plett et al., 2014; Patkar et al., 2015; Gimenez-Ibanez et al., 2016; Stringlis et al., 2018).

The ascomycete fungus *Colletotrichum higginsianum* causes anthracnose disease in numerous wild and cultivated members of the Brassicaceae, including *Arabidopsis thaliana*. The interaction of *C. higginsianum* with *A. thaliana* provides a model pathosystem in which both partners are amenable to genetic manipulation and rich genetic resources are available for the plant host. Infection of *A. thaliana* by *C. higginsianum* is characterized by an early symptomless biotrophic phase followed by a destructive necrotrophic phase (O'Connell et al., 2004). As with other hemibiotrophic pathogens, it is assumed that during the biotrophic phase the fungus manipulates living host cells to evade plant defences, while in the necrotrophic phase fungal toxins and degradative enzymes are secreted to kill host cells and mobilize nutrients (Collemare et al., 2019). We previously reported that *C. higginsianum* tightly regulates the expression of secondary metabolism biosynthetic gene clusters (BGCs) at different stages of the infection process (Dallery et al., 2017). Remarkably, no fewer than 14 BGCs are specifically induced early, during penetration and biotrophic colonization, whereas only five are preferentially activated during necrotrophy. Hence, not including possible biosynthetic intermediates, up to 14 different secondary metabolites are potentially delivered to the first infected host cell, where they may contribute to establishing a biotrophic interaction with *A. thaliana*. The transient production of these fungal metabolites exclusively in planta presents a major challenge to their structural characterization and functional analysis. In the past decade, deleting proteins involved in shaping the chromatin landscape has allowed the isolation of numerous novel metabolites from diverse axenically grown fungi (e.g. Bok et al., 2009; Studt et al., 2016; Wu et al., 2016; Fan et al., 2017). Recently, we reported a ΔcclA mutant of *C. higginsianum* affected in the trimethylation of histone proteins at H3K4 residues, which overproduces 12 different metabolites belonging to three terpenoid families, including five new molecules (Dallery et al., 2019a, b).

Despite the huge efforts made in recent years to characterize the natural products produced by plant-associated microorganisms, to date most studies have reported on only their antimicrobial activity or phytotoxicity, and have neglected their potential activity against components of the PTI response and hormone signalling (Collemare et al., 2019). Indeed, only 30 chemical screens relating to plant biology

have been reported in the literature, of which nine tested activity on plant immunity and only one concerned JA signalling (Meesters et al., 2014; Serrano et al., 2015). Using a forward chemical genetic screen, we here identify a fungal natural product that suppresses JA-mediated plant defences. Using different JA-reporter lines in Arabidopsis, we show that higginsianin B, a terpenoid metabolite produced by *C. higginsianum*, can prevent the methyl jasmonate (MeJA)-dependent degradation of JAZ repressor proteins. Three structural analogues of higginsianin B were found to lack this activity, providing clues to the structure-activity relationship and suggesting candidate functional groups that could help in identifying target binding sites. We also found that the active metabolite is able to inhibit the plant developmental signalling pathway mediated by auxin. Finally, we present evidence that higginsianin B is likely to exert its activity through inhibition of the 26S proteasome. Taken together, our work highlights the importance of fungal secondary metabolites in manipulating plant hormone signalling.

## Methods

### Biological materials

The *Colletotrichum higginsianum* wild-type strain (IMI 349063A) was maintained on Mathur's medium as previously described (O'Connell et al., 2004). *Arabidopsis thaliana* accession Columbia (Col-0) was used as the wild-type line and served as the genetic background for the previously described reporters used in this study: VSP1p:GUS (Zheng et al., 2006), PR1p:GUS (Shapiro and Zhang, 2001), CaMV35Sp:JAZ1-GUS (Thines et al., 2007), CaMV35Sp:Jas9-VENUS-NLS (Larrieu et al., 2015), JAZ10p:GUSPlus (Acosta et al., 2013), and DR5p:GUS (Ulmasov et al., 1997). Unless otherwise specified, Arabidopsis was grown axenically in half-strength Murashige and Skoog (MS) medium [0.5× MS, 0.5 g/l⁻¹ 2-(N-morpholino)ethanesulfonic acid hydrate, pH 5.7]. For solid medium, agar was added at 0.7% or 0.85% for horizontal or vertical growth, respectively.

### Chemicals

*Colletotrichum higginsianum* compound fractions were generated by purifying crude culture extracts using flash chromatography. The pure secondary metabolites used in this study, namely the diterpenoids higginsianin A, B, and C, and 13-epi-higginsianin C, were isolated and structurally identified as previously reported (Dallery et al., 2019b). All fractions and pure compounds were dissolved in dimethyl sulfoxide (DMSO) as stock solutions.

### Quantitative assay for inhibition of JA and SA responses

Hydroponically grown 12-day-old transgenic Arabidopsis seedlings of VSP1p:GUS and PR1p:GUS reporters were used to identify compounds interfering with JA- or SA-mediated defences, respectively. Seedlings were treated with the compounds for 1 h before induction of reporter gene expression with MeJA (100 µM) or SA (200 µM) dissolved in DMSO. After 24 h, the liquid medium was carefully removed from the wells with a vacuum pump. Seedlings were incubated with 150 µl lysis buffer containing 50 mM sodium phosphate, pH 7.0, 10 mM EDTA, 0.1% Triton X-100, and 1 mM 4-methylumbelliferyl-β-D-glucuronide (4-MUG; 69602, Sigma-Aldrich) at 37 °C for 90 min. The reaction was stopped by adding 50 µl of 1 M Na₂CO₃, and 4-MU fluorescence was measured in a microplate reader (excitation and emission wavelengths 365 and 455 nm, respectively). Activity was expressed as relative light units. Each treatment was performed on five independent seedlings.
### Histochemical GUS staining

Samples were fixed in 90% acetone on ice for 1 h, washed in 50 mM NaPO₄ buffer, pH 7.0, vacuum infiltrated with β-glucuronidase (GUS) substrate solution [50 mM NaPO₄ buffer, pH 7.0, 0.1% (v/v) Triton X-100, 3 mM K₃Fe(CN)₆, 1 mM 5-bromo-4-chloro-3-indolyl β-D-glucuronide], and incubated at 37 °C for 2 h. Staining was stopped with 70% ethanol and samples were mounted in 70% glycerol for observation with a binocular microscope.

### In vivo Jas9-VENUS degradation

Inhibition of JAZ protein degradation upon MeJA treatment was assayed using the *Arabidopsis* jasmonoyl isoleucine (JA-Ile) sensor CaMV35Sp:Jas9-VENUS-NLS (Larrieu *et al.*, 2015). After seed stratification for 2 days at 4 °C, seedlings were grown vertically for 5 days. Growth conditions were 21 °C with a photoperiod of 14 h light (100 µmol·m⁻²·s⁻¹). Seedlings were pre-treated with either mock solution (DMSO in 0.5× MS) or the compound under analysis (30 µM) in a sterile dish for 30 min. Then, samples were mounted in 60 µl of 30 µM MeJA in 0.5× MS on microscope slides and imaged immediately (0 min) and 30 min after MeJA treatment. The procedure enabled the evaluation of reporter expression in individual seedling roots before and after the treatments (n=10 for each condition). To ensure that the pre-treatments did not cause reporter degradation, a full sample set was also pre-treated directly on microscope slides and imaged at 0 min and after 30 min. VENUS fluorescence in living roots was imaged with a Zeiss LSM 700 confocal laser scanning microscope with 488 nm excitation and 490–555 nm emission wavelengths. All images shown within one experiment were taken with identical settings. Image processing was done with FIJI (http://fiji.sc/Fiji).

### Monitoring Jas9-VENUS degradation by immunoblot assay

Five-day-old seedlings were grown horizontally in axenic conditions on a nylon mesh (200 µm pore size) supported on MS solid medium. Growth conditions were 21 °C with a photoperiod of 14 h light (100 µmol·m⁻²·s⁻¹). Pre-treatment and treatment of seedlings was performed as described above for microscopy, except that treatments were performed in sterile dishes. E-64, a highly selective cysteine protease inhibitor (E3132, Sigma-Aldrich) and epoxomicin, a specific proteasome inhibitor (E3652, Sigma-Aldrich) were used as controls. Seedlings were snap-frozen in liquid nitrogen and kept frozen for disruption using 3 mm diameter tungsten beads in a Qiagen TissueLyser II operating at 30 Hz, 2×1 min. Total proteins from 120 seedlings were extracted with 150 µl of extraction buffer [50 mM Tris-HCl pH 7.4, 80 mM NaCl, 0.1% Tween 20, 10% glycerol, 10 mM dithiothreitol, 2× Protease inhibitor cocktail (11873580001, Roche), 5 mM PMSF]. Prior to protein quantification, debris was removed by centrifugation at 16 210 g for 10 min. Total proteins (40 µg) were separated using SDS-PAGE (10% acrylamide) and then blotted on to nitrocellulose membranes (1620112, Bio-Rad). Jas9-VENUS and ACTIN were detected using the mouse monoclonal antibodies anti-GFP 1:1000 (11814460001, Roche) or anti-actin 1:2000 (A0480, Sigma-Aldrich), respectively. The secondary antibody was an anti-mouse coupled to HRP 1:10 000 (W4021, Promega). Detection was performed with the Pico Plus system (34580, Thermo Scientific) and X-ray films (47410 19284, Fujifilm).

### Wounding assays

Horizontally grown 5-day-old *JAZ10p:GUSPlus* reporter seedlings were pre-treated with either 30 µM DMSO (mock) or 30 µM higginsianin B in water 30 min before mechanical wounding of one cotyledon as described by Acosta *et al.* (2013). Pre-treatment was performed by applying 0.5 µl of test solutions to both cotyledons of

all seedlings. Histochemical GUS staining was performed 2 h after wounding (n=60 per condition). Alternatively, 1 h after mechanical wounding of one cotyledon, the shoots and roots were collected separately for quantitative real-time (qRT)-PCR analysis of *JAZ10* expression as described previously (Acosta *et al.*, 2013). RNA and cDNA were prepared as described in Gfeller *et al.* (2011). qRT-PCR was performed as described in Chauvin *et al.* (2013) using the primers for *JAZ10* (At5g13220) and *UBC21* (At5g25760) previously reported in Gfeller *et al.* (2011).

### In vitro proteasome activity assays

To assess the direct inhibition of proteasomal subunits by higginsianin B, human newborn foreskin (BJ) normal fibroblast cells were lysed by using a lysis buffer containing 0.2% Nonidet P-40, 5 mM ATP, 10% glycerol, 20 mM KCl, 1 mM EDTA, 1 mM dithiothreitol, and 20 mM Tris, pH 7.6. Protein concentration was determined before treatment with increasing concentrations of higginsianin B or one of two known proteasome inhibitors (bortezomib or epoxomicin). Chymotrypsin-like (LLVY) and caspase-like (LLE) activities were determined by recording the hydrolysis of fluorogenic peptides Suc-Leu-Leu-Val-Tyr-AMC and Z-Leu-Leu-Glu-AMC, respectively (excitation and emission wavelengths 350 and 440 nm, respectively).

### Cell-based proteasome activity assays

Measurement of proteasome peptidase activities following exposure of cells to the compounds was performed as described previously (Sklirou *et al.*, 2015). Briefly, cells were plated in 60 mm dishes, left to adhere overnight, and then treated with the test compounds for 24 or 48 h. The cells were then lysed and proteasome activities were assayed as described above.

### Auxin treatment

Five-day-old *DR5p:GUS* auxin reporter seedlings were grown vertically as described above. Pre-treatment with mock (DMSO in 0.5× MS) or higginsianin B (30 µM in 0.5× MS) solution was performed in sterile dishes for 30 min, followed by 2 h treatment with either mock solution or naphthaleneacetic acid (NAA, 5 µM in 0.5× MS), a synthetic auxin analogue.

### Oxidative burst assay

Luminol-based oxidative burst measurement was performed with 10-day-old *A. thaliana* Col-0 seedlings in 96-well white microplates (Serrano *et al.*, 2007). Seedlings submerged in fresh 100 µl H₂O were treated with higginsianin B or DMSO 1 h before the application of 0.1 µM flg22. For a negative control, no flg22 was supplied. Luminescence was detected over a period of 60 min in a luminometer (Centro LB960, Berthold Technologies) using 100 µM luminol (L-012, Wako Chemicals) together with 10 µg·ml⁻¹ horseradish peroxidase (P6782, Sigma-Aldrich). Six biological replicates were used for each measurement.

### Statistical analyses

Statistical analyses were conducted using R software (version 3.4.2) and the packages *Rcmdr* (version 2.4-4) and *conover.test* (version 1.1.5), all available from The Comprehensive R Archive Network (CRAN; https://cran.r-project.org). The statistical significance of compound treatments on *VSP1p:GUS* and *PR1p:GUS* activation was performed using the Kruskal-Wallis test followed by multiple comparisons using the Conover-Iman test with Benjamini-Hochberg adjustment of *P*-values for false discovery rate. All proteasome activity tests were performed at least in duplicate and data were statistically analysed with a one-way ANOVA.
# Results

## Chemical genetics screens identify an inhibitor of JA signalling

Chemical genetics screens using transgenic Arabidopsis lines expressing suitable reporter genes are powerful tools to detect small molecules interfering with components of plant defence and hormone signalling (Meesters and Kombrink, 2014; Serrano et al., 2015). To search for such activities among C. higginsianum metabolites, we generated a small library of partially purified fractions (F1–F4) and one pure molecule, namely higginsianin B, isolated from liquid cultures of the C. higginsianum ΔcclA mutant (Dallery et al., 2019b). These were then screened for potential inhibitory activity against SA- and JA-induced defence responses using transgenic plants expressing the GUS reporter under the SA-responsive PATHOGENESIS RELATED 1 (PR1) promoter or the JA-responsive VEGETATIVE STORAGE PROTEIN 1 (VSP1) promoter (Shapiro and Zhang, 2001; Zheng et al., 2006). Seedlings grown hydroponically in 96-well plates were first treated with fungal metabolites before inducing the expression of the reporter genes with SA or MeJA, respectively. The use of 4-MUG as GUS substrate allowed the fluorimetric quantification of reporter gene expression in intact plants (Halder and Kombrink, 2015).

Under our experimental conditions, none of the tested compounds was able to inhibit or enhance the SA-mediated activation of *PR1p:GUS* (see Supplementary Fig. S1 at JXB online). Although seedlings pre-treated with fraction F4 and higginsianin B showed a higher activation of *PR1p:GUS* compared with the DMSO pre-treated control, these differences were not significant (adjusted *P*=0.25, Kruskal–Wallis with Conover–Iman test). In contrast, fraction F3 tended to reduce the MeJA-dependent inducibility of *VSP1p:GUS* expression by 14% (although this value was not statistically significant at the alpha level of 0.01; *P*=0.028), whereas fraction F4 robustly reduced reporter inducibility by 66% compared with mock pre-treated controls (Fig. 1A). Purification of compounds from fractions F3 and F4 identified higginsianin B as the only active metabolite at a concentration of 30 µM. In agreement with this result, comparison of HPLC chromatograms of fractions F1–F4 showed that higginsianin B was present only in fractions F3 and F4 (Supplementary Fig. S2). Control seedlings that were not treated with MeJA (i.e. uninduced) displayed only basal activation of *VSP1p:GUS* (8% of the level in induced seedlings; Fig. 1A). Using this assay, we also found that higginsianin B reduced *VSP1p:GUS* activation in a dose-dependent manner between 3 and 100 µM, with maximal inhibition of 56% at 100 µM (Fig. 1B). Given the pronounced inhibitory effect of higginsianin B on the JA pathway, we investigated this activity further.

## Higgsinsianin B inhibits JAZ1 degradation

To validate the results of the primary screen, we tested the effect of higgsinsianin B on a different marker of the JA pathway, using a transgenic *A. thaliana* line constitutively expressing the JASMONATE ZIM DOMAIN PROTEIN 1

<figure><img src="image_3.png" /></figure>

<figure><img src="image_4.png" /><figcaption>**Fig. 1.** Primary screening identified higgsinsianin B as a potential inhibitor of JA-mediated plant defence signalling. (A) Arabidopsis seedlings expressing GUS under the *VSP1* promoter, a marker of JA-mediated plant defences, were pre-treated with metabolite fractions (100 µg·ml⁻¹) or pure compounds (30 µM) for 1 h before MeJA treatment (100 µM for 24 h). Bars represent mean ±SD *VSP1p:GUS* activity of five independent seedlings from one representative experiment performed twice. (B) Dose-dependent inhibition of *VSP1p:GUS* activity by higgsinsianin B pre-treatment. Bars represent mean ±SD *VSP1p:GUS* activity of 12 independent seedlings from one representative experiment performed twice. RLU, Relative light unit. **P**<0.01, ***P**<0.001 (adjusted *P*-values; Kruskal–Wallis with Conover–Iman test).</figcaption></figure>

(JAZ1) fused to GUS (*CaMV35Sp:JAZ1-GUS*) (Thines et al., 2007). JAZ proteins repress JA-responsive genes by binding to and inhibiting transcriptional activators such as MYC2 (Pauwels and Goossens, 2011). The bioactive JA-Ile conjugate mediates the binding of JAZ proteins to the F-box protein CORONATINE INSENSITIVE1 (COI1), a member of the Skp1/Cullin1/F-box protein COI1 (SCF<sup>COI1</sup>) complex (Fonseca et al., 2009). JAZ proteins are then polyubiquitinated prior to degradation by the 26S proteasome (Chini et al., 2007; Thines et al., 2007; Fonseca et al., 2009). We therefore monitored JAZ1-GUS protein degradation in roots pre-treated with test compounds and then treated with MeJA as described previously (Meesters et al., 2014). While MeJA treatment triggered JAZ1-GUS degradation in mock pre-treated roots, higgsinsianin B pre-treatment at concentrations as low as 0.3 µM prevented the MeJA-induced degradation of JAZ1-GUS protein,
<figure><img src="image_5.png" /><figcaption>**Fig. 2.** Inhibition of JA-mediated degradation of JAZ1-GUS protein by higginsianin B. The constitutively expressed JAZ1-GUS chimeric protein was not degraded by mock pre-treatment (60 min) followed by mock treatment (30 min), as shown in seedling roots (upper row) whereas MeJA treatment triggered JAZ1-GUS degradation in mock pre-treated roots (first column, middle row). Pre-treatments with increasing concentrations of higginsianin B prevented MeJA-mediated degradation of chimeric proteins in a dose-dependent manner. The use of 10 µM JA-Ile as an inducer instead of 10 µM MeJA gave similar results, indicating that higginsianin B does not inhibit the conversion of inactive MeJA into the active JA-Ile (lower row). The proteasome inhibitor MG132 was used as a known inhibitor of JAZ1-GUS degradation. Each treatment was performed on at least five seedlings and one representative image is presented for each treatment.</figcaption></figure>

in a manner similar to the proteasome inhibitor MG132 (Fig. 2), which is known to prevent JAZ1-GUS degradation (Meesters et al., 2014). Higginsianin B may therefore either inhibit proteasome-mediated degradation of JAZ1 or block the conversion of inactive MeJA into active JA-Ile. In Arabidopsis, this conversion is a two-step process involving a methyljasmonate esterase, which produces JA from MeJA, and a jasmonoyl-L-amino acid synthetase called JAR1, which converts JA to JA-Ile (Staswick and Tiryaki, 2004). When we used active JA-Ile instead of MeJA, higginsianin B was still able to inhibit JAZ1-GUS degradation, suggesting that the molecule acts downstream of JA-Ile biosynthesis (Fig. 2).

Inhibition of JAZ degradation is specific to higginsianin B

To verify whether higginsianin B could inhibit JAZ protein degradation in vivo, we monitored its effect on the roots of reporter seedlings constitutively expressing the JA sensor Jas9-VENUS (J9V), consisting of the JAZ9 degron domain (Jas) fused to the VENUS yellow fluorescent protein and a nuclear localization signal (Larrieu et al., 2015). Seedling roots were pre-treated with either mock solution or compounds under analysis for 30 min, before being treated with MeJA for another 30 min. As expected, MeJA treatment following mock pre-treatment induced J9V reporter degradation, as indicated by the low fluorescence intensity visible in root cell nuclei following the 30 min treatment (Fig. 3A). In contrast, root pre-treatment with higginsianin B (30 µM) strongly inhibited MeJA-induced J9V degradation (Fig. 3A). To assess structure-activity relationships, we also tested three other molecules that are structurally related to higginsianin B, namely higginsianin A, higginsianin C, and 13-epi-higginsianin C (Dallery et al., 2019b). Pre-treatment with each of these compounds failed to prevent MeJA-induced J9V degradation (Fig. 3A), indicating that the inhibitory effect is specific to higginsianin B. Comparison of the structures of these molecules (Fig. 3B) suggested that the functional groups most likely to be required for inhibitory activity are the hydroxyl and/or the 4-isoheptenyl moieties of the bicyclic core.

To further validate the results obtained from live-cell imaging, we monitored J9V reporter degradation in planta by immunoblot assay. Arabidopsis seedlings were pre-treated with either mock solution or one of the four higginsianins for 30 min and subsequently treated with mock or MeJA for 30 min. While MeJA triggered J9V degradation in mock pre-treated seedlings, pre-treatment with higginsianin B at 30 µM prevented J9V degradation (Fig. 3C). The three other members of this compound family were again inactive at the same concentration (Supplementary Fig. S3). A dose-dependency test showed that higginsianin B was active at a concentration of 10 µM (Fig. 3D). As controls in this assay, E-64, a highly selective cysteine protease inhibitor, was used as an inhibitor of non-proteasomal proteases, and epoxomicin was used as a specific inhibitor of the proteasome. Similar to higginsianin B, epoxomicin prevented J9V degradation, whereas E-64 did not (Supplementary Fig. S3).
<figure><img src="image_6.png" /></figure>

<figure><img src="image_7.png" /></figure>

<figure><img src="image_8.png" /><figcaption>**Fig. 3.** Effect of higginsianin B on Jas9-VENUS (J9V) degradation and structure-activity relationship with other molecules of the higginsianin family. (A) Primary roots expressing the JA sensor J9V before and after pre-treatment with the indicated compounds (30 µM) followed by treatment with MeJA (30 µM). In the control experiment, mock pre-treatment did not induce reporter degradation, whereas MeJA treatment for 30 min was sufficient to induce J9V degradation, as indicated by the absence of reporter fluorescence. In contrast, when plants were pre-treated for 30 min with higginsianin B, MeJA treatment was no longer able to promote J9V degradation. Other members of the higginsianin family were unable to prevent MeJA-induced J9V degradation at the tested concentration (30 µM). (B) Chemical structures of higginsianin B, C, and A, and 13-epi-higginsianin C. The differing parts of the molecules are highlighted in blue. (C, D) Immunoblot analysis of MeJA-induced degradation of J9V (assayed with anti-GFP antibodies). Each lane was loaded with 40 µg of total protein extracts from 60 seedlings. ACTIN (assayed with anti-actin antibodies) and Ponceau S represent loading controls. Protein molecular mass is shown on the right. (C) Pre-treatment with higginsianin B (30 µM) reduced MeJA-induced J9V degradation. (D) Dose-dependent inhibition of MeJA-induced J9V degradation by higginsianin B.</figcaption></figure>
## *Higginsianin B inhibits wound-induced JAZ10 activation in roots*

So far, our findings revealed that higginsianin B can inhibit JAZ degradation and JA-induced gene expression resulting from exogenous MeJA treatment. To test whether the effect of higginsianin B also extends to suppressing endogenous JA-mediated responses, we assayed JA marker gene expression following mechanical wounding of seedlings pre-treated with higginsianin B. Mechanical wounding of seedling cotyledons is a strong elicitor of JA-dependent gene expression in both shoots and roots, including the activation of the JA-dependent reporter *JAZ10p:GUSPlus* (Acosta et al., 2013). Pre-treatment of seedling cotyledons with either mock solution or higginsianin B did not cause reporter activation, while mechanical wounding effectively induced *JAZ10p:GUSPlus* expression in wounded shoots in both pre-treatments (Fig. 4A). Interestingly, mock pre-treated samples also showed increased *JAZ10p:GUSPlus* expression in their roots, whereas higginsianin B pre-treatment reduced the wound-induced reporter activation in this organ (Fig. 4A). Quantification of *JAZ10* transcripts further confirmed that higginsianin B pre-treatment reduced wound-induced *JAZ10* accumulation in both shoots and roots, compared with mock treatments (Fig. 4B). Furthermore, higginsianin B pre-treatment strongly reduced MeJA-induced *JAZ10p:GUSPlus* activation in seedling roots (Fig. 5A). Taken together, these results indicate that higginsianin B can suppress endogenous JA-mediated responses.

## *Higginsianin B affects auxin-mediated signalling*

The degradation of JAZ proteins is executed by the 26S proteasome upon polyubiquitination by the SCF<sup>COI1</sup> complex (Chini *et al.*, 2007; Thines *et al.*, 2007). Likewise, the 26S proteasome is also involved in auxin perception by co-receptors, the SCF<sup>TIR1/AFB</sup> ubiquitin ligases, and their targets, the AUX/IAA family of auxin response inhibitors (Gray *et al.*, 2001; Tiwari *et al.*, 2001). If higginsianin B blocks JAZ degradation by inhibiting proteasome activity, we reasoned that it may also impact other proteasome-dependent plant responses such as auxin signalling. Treatment of seedling roots with the synthetic auxin NAA induces the expression of the auxin reporter *DR5p:GUS* in the elongation zone (Liu *et al.*, 2017) (Fig. 5B). Although higginsianin B pre-treatment alone had no visible effects on the *DR5p:GUS* expression pattern, this pre-treatment not only abolished NAA-mediated reporter induction in the root elongation zone but also reduced *DR5p:GUS* expression in the quiescent centre and root columella (Fig. 5B). This finding supports the hypothesis that higginsianin B could affect other proteasome-dependent processes, such as the activation of auxin signalling.

### *The 26S proteasome is a target of higginsianin B*

The impact of higginsianin B on JA- and auxin-mediated signalling pathways suggested the ubiquitin-proteasome system as a possible target. Therefore, to investigate whether higginsianin B can directly inhibit proteolytic activities of the

<figure><img src="image_9.png" /><figcaption>**Fig. 4.** Effect of higginsianin B on wound-induced *JAZ10p:GUSPlus* activation. (A) Horizontally grown 5-day-old *JAZ10p:GUSPlus* reporter seedlings were pre-treated with 30 µM DMSO (mock) or 30 µM higginsianin B by applying 0.5 µl of the pre-treatment solution to their cotyledons for 30 min, after which one cotyledon was mechanically wounded (as indicated by orange asterisks). GUS staining was performed 2 h after wounding. Bars=0.5 mm. (B) qRT-PCR of *JAZ10* expression following 30 min pre-treatments with mock solution or higginsianin B (higB) combined with mechanical wounding. Shoots and roots were collected independently 1 h after wounding of the aerial organs. *JAZ10* transcript levels were normalized to those of *UBC21* and are displayed relative to the expression of mock controls. Bars represent the means ±SD of three biological replicates, each containing a pool of organs from ~60 seedlings. ns, Not significant (*P*=0.08); \* *P*<0.05 (t-test).</figcaption></figure>
<figure><img src="image_10.png" /><figcaption>**Fig. 5.** Higginsianin B reduces JA- and auxin-triggered gene expression. (A) Higginsianin B pre-treatment abolished the MeJA-mediated induction of *JAZ10p:GUSPlus* in Arabidopsis roots. (B) Similarly, higginsianin B also inhibited naphthaleneacetic acid (NAA)-mediated induction of the auxin reporter *DR5p:GUS*. Note the absence of *DR5p:GUS* staining in the elongation zone of higginsianin B pre-treated/NAA-treated roots (orange asterisks) and reduced reporter expression in the meristem (pink asterisks). Pre-treatments consisted of 30 min incubation with DMSO or 30 µM higginsianin B; treatments consisted of 2 h incubation with MeJA or NAA. Bars=50 µm.</figcaption></figure>

26S proteasome *in vitro*, human cell lysates containing intact proteasomes were treated with increasing concentrations of the molecule and proteasome activity was measured. Two highly specific proteasome inhibitors, bortezomib and epoxomicin, were used as positive controls. We found that higginsianin B inhibited the chymotrypsin-like activity of the proteasome in a dose-dependent manner, with a maximal inhibition of 40% reached at 5 µM; both bortezomib and epoxomicin were more active in this assay (Fig. 6A). Higginsianin B also inhibited the caspase-like proteasomal activity at concentrations of 1 and 5 µM, similar to the level of inhibition achieved with epoxomicin and bortezomib (Fig. 6B). To measure the effect of higginsianin B on proteasome activities in cell-based assays, we used normal human diploid fibroblasts (BJ cells). In cells treated for 24 h or 48 h with higginsianin B, the compound reduced both chymotrypsin-like and caspase-like activities in a dose-dependent manner. In cells treated with 100 µM higginsianin B, the chymotrypsin-like activity was reduced to ~60% at 24 h and ~50% at 48 h relative to the control (Fig. 6C). Caspase-like activity was strongly reduced to 35% of the control at 24 h, but only to 70% of the control at 48 h (Fig. 6D). Overall, these results suggest that higginsianin B is a potent inhibitor of proteasome proteolytic activities.

### Higginsianin B inhibits PAMP-triggered production of reactive oxygen species

Protein turnover by the ubiquitin-proteasome system is involved in numerous aspects of plant immunity, from pathogen recognition to downstream defence signalling (Marino et al., 2012). For example, the analysis of proteasomal mutants has shown that early PTI responses such as the rapid production of reactive oxygen species (ROS) (“oxidative burst”) partially depends on the plant proteasome (Üstün et al., 2016). To test whether higginsianin B can also inhibit this early PTI response, we measured the flg22-induced oxidative burst in Arabidopsis seedlings based on the H₂O₂-dependent luminescence of luminol (Keppler et al., 1989). Higginsianin B inhibited the flg22-induced oxidative burst in a dose-dependent manner (Fig. 7).

## Discussion

To date, few chemical genetic screens have been used to systematically search for molecules interfering with components of plant immunity (Serrano et al., 2015; Dejonghe and Russinova,
<figure><img src="image_11.png" /><figcaption>B</figcaption></figure>

<figure><img src="image_12.png" /><figcaption>C</figcaption></figure>

<figure><img src="image_13.png" /><figcaption>D</figcaption></figure>

<figure><img src="image_14.png" /><figcaption>**Fig. 6.** Inhibition of 26S proteasome activities by higginsianin B. (A, B) *In vitro* direct inhibition of chymotrypsin-like (A) and caspase-like (B) activities in a dose-dependent manner by higginsianin B and two known proteasome inhibitors, epoxomicin and bortezomib. (C, D) Cell-based assays showing dose-dependent inhibition of chymotrypsin-like (C) and caspase-like (D) proteasomal activities in BJ cells exposed to higginsianin B for 24 h and 48 h. Data points correspond to the mean ±SD of two independent experiments. FLU, Fluorescence unit. \*P<0.05, \*\*P<0.01 (ANOVA).</figcaption></figure>

2017). The first small molecule found to inhibit JA-mediated responses in a chemical screen was Jarin-1, a plant-derived alkaloid that was subsequently shown to specifically inhibit the activity of the JA-Ile synthetase JAR1, thereby blocking the conversion of JA into bioactive JA-Ile (Meesters et al., 2014). Adopting a similar approach combined with bioassay-guided purification to screen secondary metabolites produced by the *C. higginsianum* ΔcclA mutant, we here identified higginsianin B as a novel inhibitor of jasmonate-induced plant defence gene expression. We showed that this diterpenoid can prevent both the activation of jasmonate signalling by exogenous MeJA (Fig. 2) and the wound-induced activation of this pathway (Fig. 4). More precisely, we showed that higginsianin B acts downstream of the enzymatic conversion of MeJA into JA-Ile by inhibiting the degradation of JAZ proteins, the key repressors of JA signalling in plants (Fig. 2). The degradation of JAZ proteins by the ubiquitin-proteasome system is essential for de-repressing plant defence genes regulated by jasmonate signalling (Chini et al., 2007; Thines et al., 2007). We present evidence that higginsianin B directly inhibits two catalytic activities of the 26S proteasome, suggesting that the molecule most likely blocks the activation of JA-mediated plant defences by inhibiting the proteasomal degradation of JAZ proteins (Fig. 6). In agreement with this proposed mode of action, we show that higginsianin B also inhibits another

proteasome-dependent process, namely the activation of auxin signalling (Fig. 5) (Gray et al., 2001).

To gain insight into the structural features of higginsianin B that are required for its activity, we tested the three other known members of this compound family, namely higginsianin A and C, and 13-epi-higginsianin C. Higginsianin B has a bicyclic core substituted by hydroxyl and 4-isoheptenyl groups. In contrast, the three other molecules have a tricyclic core structure, with the third ring being a tetrahydrofuran substituted by either an isobutenyl group in the case of higginsianin A or an isopropanol group in the case of higginsianin C and 13-epi-higginsianin C (Fig. 3B). Remarkably, higginsianin B was the only molecule to show activity in JAZ degradation assays (Fig. 3A), suggesting that either the hydroxyl or the 4-isoheptenyl substituents of the bicyclic core (or both) contribute to its activity. On the other hand, a second hydroxyl group located on the pyrone ring in all higginsianins is unlikely to contribute to this activity, and is therefore a good candidate for tagging higginsianin B with a fluorescent probe for direct visualization of the active metabolite by live-cell imaging. This group could also be exploited for the covalent immobilization of higginsianin B on to a solid support to search for potential protein targets by affinity purification.

While many natural proteasome inhibitors have been discovered from actinobacteria, few have been identified from fungi.
<figure><img src="image_15.png" /><figcaption>**Fig. 7.** Higginsianin B inhibits PAMP-triggered accumulation of reactive oxygen species in a dose-dependent manner. Arabidopsis seedlings were pre-treated with DMSO (mock) or higginsianin B (higB; final concentration 3–100 µM) for 60 min before the addition of 0.1 µM flg22 together with 100 µM luminol and 10 µg·ml⁻¹ horseradish peroxidase. Luminescence was continuously monitored over 60 min. Representative data from one independent experiment are shown. Each data point represents the mean of six replicates. Error bars represent the standard error of the mean.</figcaption></figure>

Those that have been identified include the peptide aldehyde fellutamide B produced by the marine fungus *Penicillium fellutalum* (Hines *et al.*, 2008) and the TMC-95 family of cyclic peptides from the soil saprophyte *Apiospora montagnei* (Momose and Watanabe, 2017). Proteasome inhibitors are currently the subject of intense interest as therapeutic agents for the control of cancer and other diseases in humans (Tsakiri and Trougakos, 2015; Wang *et al.*, 2018). In this regard, it is interesting to note that higginsianin B was recently shown to have antiproliferative activity against glioma, carcinoma, and melanoma cell lines (Cimmino *et al.*, 2016). As a novel proteasome inhibitor, higginsianin B therefore merits further investigation as a lead compound for the development of potential therapeutic applications.

The ubiquitin-proteasome system plays a critical role in multiple components of plant immunity (Marino *et al.*, 2012), and is targeted by both protein and chemical effectors of a broad range of plant pathogens to promote virulence (Üstün *et al.*, 2016). For example, *Pseudomonas syringae* pv. *syringae* secretes the nonribosomal peptide syringolin A, which binds valently to catalytic subunits of the 26S proteasome to inhibit their activity and suppress plant defences (Groll *et al.*, 2008; Misas-Villamil *et al.*, 2013). Two related bacterial Type III secreted effector proteins, XopJ from *Xanthomonas campestris* pv. *vesicatoria* and HopZ4 from *P. syringae* pv. *lachrymans*, both attenuate SA-mediated defence by inhibiting proteasome activity through their interaction with RPT6, the ATPase subunit of the 19S regulatory particle of the 26S proteasome (Üstün *et al.*, 2016). Although we have shown here that higginsianin B can directly inhibit two catalytic activities of the mammalian

proteasome (Fig. 6), further studies are now needed to determine which components of the plant proteasome are the targets of this fungal metabolite, and the nature of their interaction.

In the context of JA-mediated defence, the proteasomal degradation of JAZ repressors is manipulated by numerous effectors from both pathogenic and mutualistic microbes. For example, the *P. syringae* Type III effectors HopZ1a and HopX1 both activate JA signalling by targeting JAZ proteins for destruction in the proteasome (Jiang *et al.*, 2013; Gimenez-Ibanez *et al.*, 2016). In contrast, the symbiotic ectomycorrhizal fungus *Laccaria bicolor* suppresses JA-mediated defences by secreting the MiSSP7 effector protein, which directly interacts with JAZ proteins to protect them from degradation in the plant proteasome (Plett *et al.*, 2014). The rice blast fungus *Magnaporthe oryzae* weakens JA-mediated plant defences by secreting the inactive hydroxylated JA (12OH-JA) and a monooxygenase enzyme called Abm that hydroxylates JA and depletes endogenous rice JA levels (Patkar *et al.*, 2015). However, to our knowledge, higginsianin B is the first example of a small molecule produced by any plant-associated fungus that suppresses plant jasmonate signalling by blocking the degradation of JAZ proteins. Our finding that higginsianin B inhibits flg22-triggered ROS production (Fig. 7) indicates that this molecule has the ability to suppress not only JA-induced defence but also early PTI responses. The dampened ROS production may be a direct consequence of proteasome inhibition by higginsianin B, but we cannot exclude the possibility that higginsianin B has more than one plant target or mode of action.

In conclusion, our findings raise the possibility that higginsianin B could function during infection as a chemical effector to suppress JA-mediated defences, which are induced during the necrotrophic phase of *C. higginsianum* infection of *Brassica* spp. and Arabidopsis (Narusaka *et al.*, 2004, 2006). Work is now ongoing to determine at what stage higginsianin B is produced during infection and to genetically test its contribution to fungal virulence and plant defence suppression.

## Supplementary data

Supplementary data are available at JXB online.

Fig. S1. Screening assay for modulation of the salicylic acid signalling pathway using a *PR1p:GUS* transgenic line.

Fig. S2. HPLC-ELSD comparison of four fractions of an active crude extract of *Colletotrichum higginsianum*.

Fig. S3. Pre-treatments with compounds structurally related to higginsianin B do not influence the MeJA-induced degradation of the JA sensor J9V.

## Acknowledgements

The authors are sincerely grateful to Erich Kombrink for valuable discussions. This work was supported by a "Chaire d'Excellence" FUNAPP grant (ANR-12-CHEX-0008-01) from the Agence Nationale de la Recherche to RJO, and a Deutsche Forschungsgemeinschaft grant (GA 2419/2-1) to DG. The RJO laboratory benefits from the support of Saclay Plant Sciences-SPS (ANR-17-EUR-0007). The funders had no role in the study design, data collection, analysis, and interpretation, or writing of the manuscript.
## References

**Acosta IF, Gasperini D, Chételat A, Stolz S, Santuari L, Farmer EE.** 2013. Role of NINJA in root jasmonate signaling. *Proceedings of the National Academy of Sciences, USA* **110**, 15473–15478.

**Bigeard J, Colcombet J, Hirt H.** 2015. Signaling mechanisms in pattern-triggered immunity (PTI). *Molecular Plant* **8**, 521–539.

**Bok JW, Chiang YM, Szewczyk E, et al.** 2009. Chromatin-level regulation of biosynthetic gene clusters. *Nature Chemical Biology* **5**, 462–464.

**Chauvin A, Caldelari D, Wolfender JL, Farmer EE.** 2013. Four 13-lipoxygenases contribute to rapid jasmonate synthesis in wounded *Arabidopsis thaliana* leaves: a role for lipoxygenase 6 in responses to long-distance wound signals. *New Phytologist* **197**, 566–575.

**Chini A, Fonseca S, Fernández G, et al.** 2007. The JAZ family of repressors is the missing link in jasmonate signalling. *Nature* **448**, 666–671.

**Cimmino A, Mathieu V, Masi M, et al.** 2016. Higginsianins A and B, two diterpenoid α-pyrones produced by *Colletotrichum higginsianum*, with *in vitro* cytostatic activity. *Journal of Natural Products* **79**, 116–125.

**Collemare J, O'Connell R, Lebrun MH.** 2019. Nonproteinaceous effectors: the *terra incognita* of plant-fungal interactions. *New Phytologist* **223**, 590–596.

**Dallery JF, Adelin É, Le Goff G, Pigné S, Auger A, Ouazzani J, O'Connell RJ.** 2019a. H3K4 trimethylation by CclA regulates pathogenicity and the production of three families of terpenoid secondary metabolites in *Colletotrichum higginsianum*. *Molecular Plant Pathology* **20**, 831–842.

**Dallery JF, Lapalu N, Zampounis A, et al.** 2017. Gapless genome assembly of *Colletotrichum higginsianum* reveals chromosome structure and association of transposable elements with secondary metabolite gene clusters. *BMC Genomics* **18**, 667.

**Dallery JF, Le Goff G, Adelin E, Iorga BI, Pigné S, O'Connell RJ, Ouazzani J.** 2019b. Deleting a chromatin remodeling gene increases the diversity of secondary metabolites produced by *Colletotrichum higginsianum*. *Journal of Natural Products* **82**, 813–822.

**Dejonghe W, Russinova E.** 2017. Plant chemical genetics: from phenotype-based screens to synthetic biology. *Plant Physiology* **174**, 5–20.

**Dodps PN, Rathjen JP.** 2010. Plant immunity: towards an integrated view of plant-pathogen interactions. *Nature Reviews Genetics* **11**, 539–548.

**Fan A, Mi W, Liu Z, Zeng G, Zhang P, Hu Y, Fang W, Yin WB.** 2017. Deletion of a histone acetyltransferase leads to the pleiotropic activation of natural products in *Metarhizium robertsii*. *Organic Letters* **19**, 1686–1689.

**Fonseca S, Chini A, Hamberg M, Adie B, Porzel A, Kramell R, Miersch O, Wasternack C, Solano R.** 2009. (+)-7-*iso*-Jasmonoyl-L-isoleucine is the endogenous bioactive jasmonate. *Nature Chemical Biology* **5**, 344–350.

**Gfeller A, Baerenfaller K, Loscos J, Chételat A, Baginsky S, Farmer EE.** 2011. Jasmonate controls polypeptide patterning in undamaged tissue in wounded Arabidopsis leaves. *Plant Physiology* **156**, 1797–1807.

**Gimenez-Ibanez S, Chini A, Solano R.** 2016. How microbes twist jasmonate signaling around their little fingers. *Plants* **5**, 9.

**Gray WM, Kepinski S, Rouse D, Leyser O, Estelle M.** 2001. Auxin regulates SCFTR1-dependent degradation of AUX/IAA proteins. *Nature* **414**, 271–276.

**Groll M, Schellenberg B, Bachmann AS, Archer CR, Huber R, Powell TK, Lindow S, Kaiser M, Dudler R.** 2008. A plant pathogen virulence factor inhibits the eukaryotic proteasome by a novel mechanism. *Nature* **452**, 755–758.

**Halder V, Kombrink E.** 2015. Facile high-throughput forward chemical genetic screening by *in situ* monitoring of glucuronidase-based reporter gene expression in *Arabidopsis thaliana*. *Frontiers in Plant Science* **6**, 13.

**Hines J, Groll M, Fahnestock M, Crews CM.** 2008. Proteasome inhibition by fellutamide B induces nerve growth factor synthesis. *Chemistry & Biology* **15**, 501–512.

**Jiang S, Yao J, Ma KW, Zhou H, Song J, He SY, Ma W.** 2013. Bacterial effector activates jasmonate signaling by directly targeting JAZ transcriptional repressors. *PLoS Pathogens* **9**, e1003715.

**Katsir L, Schilmiller AL, Staswick PE, He SY, Howe GA.** 2008. COI1 is a critical component of a receptor for jasmonate and the bacterial virulence factor coronatine. *Proceedings of the National Academy of Sciences, USA* **105**, 7100–7105.

**Keppler LD, Baker CJ, Atkinson MM.** 1989. Active oxygen production during a bacteria-induced hypersensitive reaction in tobacco suspension cells. *Phytopathology* **79**, 974–978.

**Larrieu A, Champion A, Legrand J, et al.** 2015. A fluorescent hormone biosensor reveals the dynamics of jasmonate signalling in plants. *Nature Communications* **6**, 6043.

**Liu Y, Sun L, Zhang P, Wan J, Wang R, Xu J.** 2017. Lanthanum inhibits primary root growth by repressing auxin carrier abundances in *Arabidopsis*. *Frontiers in Plant Science* **8**, 1661.

**Marino D, Peeters N, Rivas S.** 2012. Ubiquitination during plant immune signaling. *Plant Physiology* **160**, 15–27.

**Meesters C, Kombrink E.** 2014. Screening for bioactive small molecules by in vivo monitoring of luciferase-based reporter gene expression in *Arabidopsis thaliana*. *Methods in Molecular Biology* **1056**, 19–31.

**Meesters C, Mönig T, Oeljeklaus J, Krahn D, Westfall CS, Hause B, Jez JM, Kaiser M, Kombrink E.** 2014. A chemical inhibitor of jasmonate signaling targets JAR1 in *Arabidopsis thaliana*. *Nature Chemical Biology* **10**, 830–836.

**Misas-Villamil JC, Kolodziejek I, Crabill E, Kaschani F, Niessen S, Shindo T, Kaiser M, Alfano JR, van der Hoorn RA.** 2013. *Pseudomonas syringae* pv. *syringae* uses proteasome inhibitor syringolin A to colonize from wound infection sites. *PLoS Pathogens* **9**, e1003281.

**Momose I, Watanabe T.** 2017. Tyropeptins, proteasome inhibitors produced by *Kitasatospora* sp. MK993-dF2. *Journal of Antibiotics* **70**, 542–550.

**Narusaka M, Abe H, Kobayashi M, Kubo Y, Narusaka Y.** 2006. Comparative analysis of expression profiles of counterpart gene sets between *Brassica rapa* and *Arabidopsis thaliana* during fungal pathogen *Colletotrichum higginsianum* infection. *Plant Biotechnology* **23**, 503–508.

**Narusaka Y, Narusaka M, Park P, et al.** 2004. *RCH1*, a locus in *Arabidopsis* that confers resistance to the hemibiotrophic fungal pathogen *Colletotrichum higginsianum*. *Molecular Plant-Microbe Interactions* **17**, 749–762.

**O'Connell R, Herbert C, Sreenivasaprasad S, Khatib M, Esquerré-Tugayé MT, Dumas B.** 2004. A novel *Arabidopsis-Colletotrichum* pathosystem for the molecular dissection of plant-fungal interactions. *Molecular Plant-Microbe Interactions* **17**, 272–282.

**Patkar RN, Benke PI, Qu Z, Chen YY, Yang F, Swarup S, Naqvi NI.** 2015. A fungal monooxygenase-derived jasmonate attenuates host innate immunity. *Nature Chemical Biology* **11**, 733–740.

**Pauwels L, Goossens A.** 2011. The JAZ proteins: a crucial interface in the jasmonate signaling cascade. *The Plant Cell* **23**, 3089–3100.

**Pieterse CM, Van der Does D, Zamioudis C, Leon-Reyes A, Van Wees SC.** 2012. Hormonal modulation of plant immunity. *Annual Review of Cell and Developmental Biology* **28**, 489–521.

**Plett JM, Daguerre Y, Wittulsky S, et al.** 2014. Effector MiSSP7 of the mutualistic fungus *Laccaria bicolor* stabilizes the *Populus* JAZ6 protein and represses jasmonic acid (JA) responsive genes. *Proceedings of the National Academy of Sciences, USA* **111**, 8299–8304.

**Serrano M, Kombrink E, Meesters C.** 2015. Considerations for designing chemical screening strategies in plant biology. *Frontiers in Plant Science* **6**, 131.

**Serrano M, Robatzek S, Torres M, Kombrink E, Somssich IE, Robinson M, Schulze-Lefert P.** 2007. Chemical interference of pathogen-associated molecular pattern-triggered immune responses in *Arabidopsis* reveals a potential role for fatty-acid synthase type II complex-derived lipid signals. *Journal of Biological Chemistry* **282**, 6803–6811.

**Shapiro AD, Zhang C.** 2001. The role of *NDR1* in avirulence gene-directed signaling and control of programmed cell death in *Arabidopsis*. *Plant Physiology* **127**, 1089–1101.

**Sklirou AD, Ralli M, Dominguez M, Papassideri I, Skaltsounis AL, Trougakos IP.** 2015. Hexapeptide-11 is a novel modulator of the proteostasis network in human diploid fibroblasts. *Redox Biology* **5**, 205–215.

**Staswick PE, Tiryaki I.** 2004. The oxylipin signal jasmonic acid is activated by an enzyme that conjugates it to isoleucine in *Arabidopsis*. *The Plant Cell* **16**, 2117–2127.

**Stringlis IA, Zhang H, Pieterse CMJ, Bolton MD, de Jonge R.** 2018. Microbial small molecules – weapons of plant subversion. *Natural Product Reports* **35**, 410–433.
Studt L, Rösler SM, Burkhardt I, Arndt B, Freitag M, Humpf HU, Dickschat JS, Tudzynski B. 2016. Knock-down of the methyltransferase Kmt6 relieves H3K27me3 and results in induction of cryptic and otherwise silent secondary metabolite gene clusters in *Fusarium fujikuroi*. *Environmental Microbiology* **18**, 4037–4054.

Thines B, Katsir L, Melotto M, Niu Y, Mandaokar A, Liu G, Nomura K, He SY, Howe GA, Browse J. 2007. JAZ repressor proteins are targets of the SCF<sup>CO1</sup> complex during jasmonate signalling. *Nature* **448**, 661–665.

Tiwari SB, Wang XJ, Hagen G, Guilfoyle TJ. 2001. AUX/IAA proteins are active repressors, and their stability and activity are modulated by auxin. The Plant Cell **13**, 2809–2822.

Tsakiri EN, Trougakos IP. 2015. The amazing ubiquitin-proteasome system: structural components and implication in aging. *International Review of Cell and Molecular Biology* **314**, 171–237.

Ulmasov T, Murfett J, Hagen G, Guilfoyle TJ. 1997. Aux/IAA proteins repress expression of reporter genes containing natural and highly active synthetic auxin response elements. The Plant Cell **9**, 1963–1971.

Üstün S, Sheikh A, Gimenez-Ibanez S, Jones A, Ntoukakis V, Börnke F. 2016. The proteasome acts as a hub for plant immunity and is targeted by *Pseudomonas* type III effectors. *Plant Physiology* **172**, 1941–1958.

Wang H, Yang Q, Dou QP, Yang H. 2018. Discovery of natural proteasome inhibitors as novel anticancer therapeutics: current status and perspectives. *Current Protein & Peptide Science* **19**, 358–367.

Wu G, Zhou H, Zhang P, et al. 2016. Polyketide production of pestaloficiols and macrodiolide ficiolides revealed by manipulations of epigenetic regulators in an endophytic fungus. *Organic Letters* **18**, 1832–1835.

Zheng W, Zhai Q, Sun J, et al. 2006. Bestatin, an inhibitor of aminopeptidases, provides a chemical genetics approach to dissect jasmonate signaling in Arabidopsis. *Plant Physiology* **141**, 1400–1413.