# In vitro neoformation of woody plants (apple, pear, weigela)

Elisabeth Chevreau, M. Dufour, Michel Duron

► To cite this version:

Elisabeth Chevreau, M. Dufour, Michel Duron. In vitro neoformation of woody plants (apple, pear, weigela). The impact of biotechnology in agriculture. The meeting point between fundamental and applied in vitro culture research, Université de Picardie Jules Verne (UPJV). FRA., Jul 1989, Amiens, France. pp.1. hal-01601221

HAL Id: hal-01601221

https://hal.science/hal-01601221

Submitted on 7 Jun 2020

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UNIVERSITE DE PICARDIE

International Conference

THE IMPACT OF BIOTECHNOLOGY
IN AGRICULTURE

THE MEETING POINT BETWEEN FUNDAMENTAL
AND APPLIED IN VITRO CULTURE RESEARCH

ABSTRACTS: POSTERS

10 - 11 - 12 JULY 1989

Palais des Congrès

AMIENS, FRANCE

WITH COLLABORATION OF:

IAPTC

SOCIETE BOTANIQUE DE FRANCE

FINANCIAL SUPPORT BY:

CONSEIL REGIONAL DE PICARDIE

CONSEIL GENERAL DE LA SOMME

MAIRIE D'AMIENS

D.A.G.I.C.

CREDIT AGRICOLE

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IN VITRO NEOFORMATION OF WOODY PLANTS
(APPLE, PEAR, WEIGELA)

E.CHEVREAU, M.DUFOUR, M.DURON

INRA, Station d'amélioration des espèces fruitières
et ornementales, Beaucouzé, 49000 ANGERS, FRANCE

Adventitious bud neoformation requires the organization of a new meristem from a few undifferentiated cells of an explant. This technique may induce variability:

- somaclonal variation if the explant has undergone undifferentiated growth.

- induced mutations if a physical or chemical mutagenic treatment has been applied to the explant.

### Apple:

Leaves from in vitro plants of several scion varieties ('Gala', 'Golden Delicious', 'Granny Smith') and rootstocks ('Mac 9', 'Lancep', 'Cepiland', 'Novole') were excised in order to produce neoformations either through a callus intermediary or directly from the lamina. Scion varieties exhibited the highest rate of regeneration (100 %); 2000 plants from 'Gala' were successfully acclimatized and are now entering field trials to evaluate possible somaclonal variation. Regeneration was also obtained from roots and internodes.

### Pear:

Regeneration was obtained from in vitro leaves of 4 pear varieties at the rate of 50 %, using Lepoivre basal medium supplemented with thidiazuron. Neoformation ability exists among the species *Pyrus communis* (varieties 'Seckel', 'Louise Bonne', 'Comice') and *Pyrus brettschneideri* (variety 'Crystal Pear').

### Weigela:

Adventitious buds were obtained on internodes from in vitro plants (varieties 'Eva Rathke', 'Bristol Ruby'). Mutants were observed following the treatment of the explants with a chemical mutagen (EMS).