# Role of the SOS response in the conjugative transfer and genome maintenance of multidrug resistance Salmonella Genomic Island 1

Marine C Pons, Axel Cloeckaert, Benoît Doublet

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Marine C Pons, Axel Cloeckaert, Benoît Doublet. Role of the SOS response in the conjugative transfer and genome maintenance of multidrug resistance Salmonella Genomic Island 1. Plasmid around the globe 2021, The International Society for Plasmid Biology and other Mobile Genetic Elements (ISPB), May 2021, Toulouse, France. **hal-03356104**

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Submitted on 27 Sep 2021

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Role of the SOS response in the conjugative transfer and genome
maintenance of multidrug resistance Salmonella Genomic Island 1

Marine C. PONS<fnref n="1" />, Axel CLOECKAERT¹², Benoît DOUBLET¹²

*Salmonella* Genomic Island 1 (SGI1) is a multidrug resistance integrative mobilizable element (IME) specifically mobilized *in trans* by IncC and IncA conjugative plasmids. SGI1 hijacks the plasmid conjugation apparatus to be mobilized. However, these mobile elements are incompatible in bacterial population. This incompatibility is caused by SGI1 transient replication that involves besides IncC/IncA factors the SGI1 regulatory region *sgaCD*. This incompatibility is partially counteracted by a SGI1-encoded Toxin-Antitoxin system (*sgiAT*).

The SOS response has been previously shown to promote the transfer of few mobile
elements. We detected two putative binding sites of LexA, the transcriptional repressor
of the SOS response, in the promoter regions of *sgiAT* and *sgaCD*. We further
demonstrated experimentally that these LexA binding sites are functional and that the
expression of *sgiAT* and *sgaCD* is regulated by the SOS response.
Further work is ongoing to investigate the role of the SOS response in the molecular
crosstalk between SGI1 and IncC/IncA plasmids.

Location May/June 2021: Nouzilly, France.

<footnote n="1">¹ INRAE, Université de Tours, ISP, F-37380, Nouzilly, France</footnote>

<footnote n="2">² PhD supervisors</footnote>