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<figure><img src="image_79.png" /><figcaption>Figure S5</figcaption></figure>

(A) Mouse infections are carried out as in the scheme in Figure 1A. NK cells were isolated from spleens of mice previously infected with *S. pneumoniae* (red bars, D21SPN NKs) or not (black bars, D21PBS NKs). Highly purified NK cells were fixed, and chromatin was extracted and sheared. ChIP for H3K4me1 were performed and resulting positive fractions of the chromatin were amplified using PCR for the indicated targets. Enrichment percentage for H3K4me1 pull-down on -34kb upstream the *gzmb* gene and intergenic regions (IG) in NK cells from D21PBS and D21SPN mice. Data are representative of at least three experiments with n ≥ 4 mice/group.

(B) Mouse infections and *in vitro* experiments are carried out as in the scheme in Figure 1A, with the exception that Prf1 KO mice were used. Purified D21PBS (black) or D21SPN (red) Prf1 KO NK cells were stimulated *in vitro* with cytokines (IL-15 at 2 ng/ml and IL-18 at 1,5 ng/ml) and formaldehyde inactivated *S. pneumoniae* (SPN, MOI 20) for 24 hours. Intensity of Granzyme B expression in NK cells (MFI, **left panel**) and percentage of Granzyme B⁺ NK cells (**right panel**) upon *in vitro* stimulation. Box plots where each dot represents a pool of mice from one experiment, line are median, error bar show min to max. Data are representative of one experiment with n = 4 pooled mice/group and n ≥ 2 experimental replicates/group.

(C-D) Mouse infections and *in vivo* transfers are carried out as in the scheme in Figure 3A, with the exception that Prf1 KO mice were used as both donor and recipient mice.

(C) Granzyme B ELISA experiment of lung supernatants from infected mice at 40h post-infection, having received either D21PBS (black) or D21SPN (red) Prf1 KO NK cells. Dots represent individual mice, bars are the mean and error bars are the standard error of the mean (SEM). Data are representative of one experiment with n ≥ 3 mice/group.

(D) Percentages of neutrophils, CD69⁺ neutrophils and NK cells in the lungs of Prf1 KO recipient mice having received either D21PBS (black) or D21SPN (red) Prf1 KO NK cells. Box plots with each dot representing individual mice, lines are the median, error bar show min to max. Data are representative of one experiment with n ≥ 3 mice/group.
ns, not significant. Mann-Whitney tests for statistical significance.