# Identification and analyses of DNA-binding with One zinc Finger (DOF) transcription factor family in Medicago truncatula

Rana Muhammad Atif, Richard Thompson, Sergio Ochatt

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Rana Muhammad Atif, Richard Thompson, Sergio Ochatt. Identification and analyses of DNA-binding with One zinc Finger (DOF) transcription factor family in Medicago truncatula. 3. Annual Computational Science Conference, May 2015, Islamabad, Pakistan. hal-01603780

HAL Id: hal-01603780

https://hal.science/hal-01603780

Submitted on 5 Jun 2020

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# Identification and analyses of DNA-binding with One zinc Finger (DOF) transcription factor family in Medicago truncatula

Rana Muhammad Atif<fnref n="1" />,<fnref n="2" />,<fnref n="3" />*, Richard Thompson<fnref n="1" />, Sergio Ochatt<fnref n="1" />

## ABSTRACT

DOF proteins are plant-specific transcription factors which have been implicated in diverse plant processes such as seed germination, seed development, carbohydrate and secondary metabolism, light mediated gene regulation, vascular tissue development, disease response, cell cycle regulation and hormone homeostasis. Owing to their importance in plant genomes, a systematic in-silico analysis was carried out to identify non-redundant genes encoding C2-C2 DOF Zn-finger domain containing transcription factor family in the model legume *M. truncatula* genome. A total of 22 *Dof* genes encoding complete DOF domain were predicted and renamed as *MtDof* (*Medicago truncatula Dof*). Gene localization studies demonstrated that putative *MtDof* genes were localized over seven out of eight *M. truncatula* chromosomes. Exon/intron structure analysis has shown some intron-less *Dof* genes which might have resulted from intron loss during evolution. Evolutionary studies suggested that tandem duplications were predominantly responsible for the expansion of the DOF TF family during evolution in *M. truncatula*. The comparison of DOF DNA-binding domain in all the MtDOF proteins revealed a high level of residue conservation with 31 out of 52 amino acids being 100% conserved in all 22 proteins. A comparative phylogenetic tree of 69 DOF protein (from Arabidopsis, pea and *M. truncatula*) revealed six distinct groups of DOF proteins. A total of 26 different conserved domains has been identified in 69 DOF proteins, indicating the gene duplication events during the evolutionary process. Some of the groups in phylogenetic tree contain single or double conserved domains, while others contain multiple conserved domains. These computational studies paved the way for better understanding the molecular evolution and functional diversity of DOF TF family in *M. truncatula*.

**Keywords:** Model legume, Zinc finger, Gene structure, Phylogenetic tree, Genome evolution,

<footnote n="1">¹INRA, UMR Agroécologie, pôle GEAPSI, 17 rue Sully, 21000 Dijon, France.</footnote>

<footnote n="2">²Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad, Pakistan.</footnote>

<footnote n="3">³Center for Advanced Studies in Food Security-Agriculture, University of Agriculture, Faisalabad, Pakistan.</footnote>