# Identification of new binding partners of the chemosensory signaling protein Gγ13 expressed in taste and olfactory sensory cells.

Zhenhui Liu, Claire Fenech, Hervé Cadiou, Sylvie Grall, Esmerina Tili, Fabienne Laugerette, Anna Wiencis, Xavier Grosmaitre, Jean-Pierre Montmayeur

► To cite this version:

Zhenhui Liu, Claire Fenech, Hervé Cadiou, Sylvie Grall, Esmerina Tili, et al.. Identification of new binding partners of the chemosensory signaling protein Gγ13 expressed in taste and olfactory sensory cells.. Frontiers in Cellular Neuroscience, 2012, 6, pp.26. 10.3389/fncel.2012.00026. hal-00716694

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# Identification of new binding partners of the chemosensory signaling protein Gγ13 expressed in taste and olfactory sensory cells

Zhenhui Liu<fnref n="1" />†, Claire Fenech<fnref n="1" />‡, Hervé Cadiou<fnref n="2" />†, Sylvie Grall<fnref n="1" />, Esmerina Tili<fnref n="1" />†, Fabienne Laugerette<fnref n="3" />, Anna Wiencis<fnref n="3" />, Xavier Grosmaitre<fnref n="2" /> and Jean-Pierre Montmayeur<fnref n="1" />,<fnref n="3" />*

**Edited by:**

Dieter Wicher, Max Planck Institute for Chemical Ecology, Germany

**Reviewed by:**

Monika Stengl, Universität Kassel, Germany

Yuanquan Song, University of California San Francisco, USA

**\*Correspondence:**

Jean-Pierre Montmayeur,
Chemosensory Perception, Unités Mixtes de Recherche, Centre des Sciences du Goût, 9E Boulevard J. D'Arc, 21000 Dijon, France.

e-mail: jean-pierre.montmayeur@u-bourgogne.fr

**†Present address:**

Zhenhui Liu, Marine Life College, Ocean University of China, 5 Yushan Road, 266003 Qingdao, China.

Hervé Cadiou, Nociception and Pain Department, Institut des Neurosciences Cellulaires et Intégratives (INCII), CNRS UPR3212, 5 Rue Blaise Pascal, F-67084 Strasbourg, France.

Esmerina Tili, Ohio State University, Comprehensive Cancer Center, 460 West 12th Ave, BRT 1060, Columbus, OH 43201, USA.

Tastant detection in the oral cavity involves selective receptors localized at the apical extremity of a subset of specialized taste bud cells called taste receptor cells (TRCs). The identification of the genes coding for the taste receptors involved in this process have greatly improved our understanding of the molecular mechanisms underlying detection. However, how these receptors signal in TRCs, and whether the components of the signaling cascades interact with each other or are organized in complexes is mostly unexplored. Here we report on the identification of three new binding partners for the mouse G protein gamma 13 subunit (Gγ13), a component of the bitter taste receptors signaling cascade. For two of these Gγ13 associated proteins, namely GOPC and MPDZ, we describe the expression in taste bud cells for the first time. Furthermore, we demonstrate by means of a yeast two-hybrid interaction assay that the C terminal PDZ binding motif of Gγ13 interacts with selected PDZ domains in these proteins. In the case of the PDZ domain-containing protein zona occludens-1 (ZO-1), a major component of the tight junction defining the boundary between the apical and baso-lateral region of TRCs, we identified the first PDZ domain as the site of strong interaction with Gγ13. This association was further confirmed by co-immunoprecipitation experiments in HEK 293 cells. In addition, we present immunohistological data supporting partial co-localization of GOPC, MPDZ, or ZO-1, and Gγ13 in taste buds cells. Finally, we extend this observation to olfactory sensory neurons (OSNs), another type of chemosensory cells known to express both ZO-1 and Gγ13. Taken together our results implicate these new interaction partners in the sub-cellular distribution of Gγ13 in olfactory and gustatory primary sensory cells.

**Keywords:** taste bud, ZO-1, Gγ13, PDZ, olfactory sensory neurons, MPDZ, GOPC

## INTRODUCTION

In rodents the peripheral gustatory system contributes to the detection of rapid molecules present in the oral cavity. This task is accomplished through taste receptors present on the apical microvilli of specialized polarized neuroepithelial taste bud cells also called taste receptor cells (TRCs) or type II cells. TRCs are one of four cell types found in the taste buds of the tongue papillae along with supporting cells (type I), presynaptic cells (type III) and basal cells (type IV) (Finger, 2005). TRCs are elongated cells extending microvilli at their apical end. These extensions which protrude from the adjacent epithelium at the taste bud pore harbor taste receptors designed to recognize the rapid compounds dissolved in saliva. At the pore, tight junctions between the cells composing the taste bud bestow polarity on the cells and seal the paracellular space thus isolating taste receptors on the apical

membrane from ion channels found on the basolateral membrane. TRPM5 and voltage-gated Na⁺ channels are the main types of channels found on the baso-lateral membrane of TRCs (Gao et al., 2009) where they are thought to play an important role in the generation of action potentials coding the properties of the tastants (Vandenbeuch and Kinnamon, 2009). Claudins and occludins are two of the main transmembrane proteins composing the tight junction (Furuse et al., 1998; Tsukita and Furuse, 1998). The selectivity of the paracellular barrier formed by tight junctions between neighboring cells is defined by the specific nature of the claudins composing it (Tsukita et al., 2008). It was reported recently that claudin 6 and 7 are found in microvilli and on the basolateral membrane of a subset of taste bud cells (TBCs) respectively while claudin 4 and 8, which are associated with a reduced cationic conductance, are prevalent at the taste

<footnote n="1">¹ Chemosensory Perception, Centre des Sciences du Goût et de l'Alimentation, UMR-6265 CNRS, UMR-1324 INRA, Dijon, France</footnote>

<footnote n="2">² Functional Plasticity of Olfactory Neurons, Centre des Sciences du Goût et de l'Alimentation, UMR-6265 CNRS, UMR-1324 INRA, Dijon, France</footnote>

<footnote n="3">³ General Olfaction and Sensing Program on a European Level, Centre des Sciences du Goût et de l'Alimentation, UMR-6265 CNRS, UMR-1324 INRA, Dijon, France</footnote>

<footnote>‡These authors contributed equally to this work.</footnote>
bud pore (Michlig et al., 2007). These proteins interact with zona occludens-1 (ZO-1), a multimodular cytoplasmic protein (Mitic and Anderson, 1998). ZO-1 was the first protein (225 kDa) shown to be specifically associated with the tight junction (Anderson et al., 1988; Stevenson and Keon, 1998). Subsequent studies identified ZO-1 isoforms as well as ZO-2 and ZO-3 as binding partners of ZO-1 (Gumbiner et al., 1991). ZO proteins belong to the large family of membrane-associated guanylate kinases (MAGUKs). All three known ZO proteins are each composed of three PDZ domains, one Src homology 3 domain (SH3), one guanylate kinase-like homologue domain (GUK) and proline-rich domains. PDZ and GUK domains interact selectively with claudins and occludins respectively (Furuse et al., 1994; Itoh et al., 1999). In addition, ZO proteins can bind to actin thus acting as scaffolds linking tight-junction proteins to the cytoskeleton (Fanning et al., 1998).

PDZ domains are typically stretches of about 100 amino acids able to recognize selectively a short peptide motif. Their role in receptor clustering and the organization of supramolecular complexes is well documented (Sheng, 1996). MPDZ also known as MUPP1, is a 13 PDZ domains-containing protein interacting selectively with a great number of PDZ binding motif-containing proteins including claudin-1 (Hamazaki et al., 2002). Single or multiple PDZ domains-containing proteins are often involved in the trafficking and localization of receptors or cytosolic signaling proteins to specialized membrane regions. A well-studied such example is the Golgi-associated protein GOPC also known as PIST. GOPC contains a single PDZ domain and two coiled-coil domains, one of which includes a leucine zipper important for homodimerization. It is known to regulate the intracellular sorting and plasma membrane location of a number of proteins (Yao et al., 2001; Cheng et al., 2002; Gentzsch et al., 2003; Hassel et al., 2003; Wente et al., 2005; Ito et al., 2006) including the adherent junction protein cadherin 23 in the highly specialized sensory hair cells of the inner ear (Xu et al., 2010).

In TRCs, bitter tastants binding to the apical membrane or membrane depolarization both lead to the secretion of adenosine 5'-triphosphate (ATP) from gap junction hemichannels located on the baso-lateral membrane (Huang and Roper, 2010). The signaling cascade downstream of taste G protein-coupled receptors (GPCRs) involves a number of well-characterized components. One of these signaling molecules is a G protein alpha subunit called gustducin (Gαgust) which plays an important role in sweet, umami, and bitter taste transduction (Gilbertson et al., 2000; He et al., 2004). Gustducin is part of an heterotrimeric complex including G beta 1 (Gβ1) and Gγ13, consequently Gγ13 much like Gαgust is abundant in a subset of type II TRCs (Huang et al., 1999; Clapp et al., 2001; Ohtubo and Yoshii, 2011). Expression of Gγ13 has also been reported in three additional types of sensory cells including retinal bipolar cells, vomeronasal, and olfactory sensory neurons (VOSNs and OSNs) (Huang et al., 2003; Kulaga et al., 2004; Kerr et al., 2008). More recently nutrient-sensing neurons of the hypothalamus were found to express Gγ13 as well (Ren et al., 2009). In OSNs Gγ13 is very abundant in cilia along with GαOlf and the guanine nucleotide exchange factor Ric-8B to which it was revealed to bind *in vitro* (Kerr et al., 2008). In TRCs, Gγ13 was reported to interact directly with the

PDZ-containing scaffolding proteins PSD95, Veli-2, and SAP97 (Li et al., 2006).

Here, we report the identification of three new interaction partners for Gγ13 with various subcellular distributions in taste cells and OSNs. Through these previously unidentified interactions our results highlight partnerships between signal transduction components and multimodular proteins implicated in macromolecular complexes with possible consequences on sensory signaling.

# MATERIALS AND METHODS

## ANIMALS

Experiments were performed on C57BI/6J mice (P0—7 weeks old). The animals were fed a standard laboratory chow ad libitum (UAR A04, Usine d'Alimentation Rationnelle, France) and housed under constant temperature and humidity with a light-dark cycle of 12 h following French guidelines for the use and care of laboratory animals. All experimental protocols were approved by the animal ethics committee of the University of Burgundy.

## EXPRESSION CONSTRUCTS

Mice were euthanized with an overdose of sodium pentobarbital and decapitated. Various tissues were collected and immediately processed for total RNA isolation using the RNAeasy kit (Qiagen, Germany) following the manufacturer's instructions. The RNA was then treated with DNase I (Promega, USA) and cleaned before reverse transcription. First strand cDNA was synthesized using 1 µg of total RNA with Superscript II (Invitrogen, USA) according to the manufacturer's protocol.

The entire open reading frame of mouse Gγ13, PDZ domains of ZO-1, Veli-2, PSD95, SAP97, RGS12, SH3 domain of ZO-1, and c-terminal intracellular regions of the junctional adhesion molecule (JAM), claudin 1, claudin 4, or claudin 8 were PCR amplified from C57BI/6J mice brain, testis, or circumvallate papillae cDNA using specific primers (Operon, Germany) containing a Sal I (forward primer) or Not I (reverse primer) restriction site. For a complete list of primers including melting temperatures and size of the expected PCR products see Table A1.

PCR reactions (25 µl) contained 1× PFU turbo buffer (Stratagene, USA), 0.4 µM of each primer, 10 µM dNTPs (Qiagen, Germany) and 1/20th of the appropriate RT reaction (water for control). Cycling parameters were: 95°C for 2 min then 35 cycles of 95°C for 30 s; appropriate melting temperature (Table A1) for 40 s, 72°C for 60 s, and final elongation at 72°C for 10 min. Following amplification (Biometra, Germany) an aliquot of the PCR products was loaded onto 1.4% agarose Seakem TAE gels (Cambrex, USA) to verify the specificity of the reaction. Single products of the expected size were then subcloned into pSTBlue-1 according to the manufacturer's directions (Novagen, USA). Recombinant clones were analyzed for accuracy by sequencing before subsequent subcloning into the Sal I and Not I sites of either pDBLeu (bait) or pEXP (prey) vectors of the Proquest two-hybrid system (Invitrogen, USA) or pDisplay-FLAG or pDisplay-HA (Invitrogen, USA) vectors. All constructs were sequenced to ensure in frame subcloning.
## YEAST TWO-HYBRID INTERACTIONS

Yeast two-hybrid interactions were performed following the recommendations of the manufacturer of the Proquest two-hybrid system (Invitrogen, USA). Briefly, the appropriate combination of bait and prey plasmids (200 ng each) were co-transformed into competent MaV203 yeast cells (Invitrogen, USA) and plated onto minimal media plates without leucine and tryptophan. The plates were incubated for 48 h at 30°C before selection of two colonies, each dissolved into 500 ml of water. To test the strength of the interaction 10 µl of each slurry was spotted side by side onto plates lacking leucine, histidine, and tryptophan but containing either 0 (control plate), 12.5, 25, or 50 mM 3-Amino-1,2,4-triazole (3-AT) (Sigma, USA). After 24 h at 30°C, the plates were replica cleaned using a velour cloth and incubated an additional 48–72 h at 30°C prior to growth assessment.

## CO-IMMUNOPRECIPITATION AND WESTERN BLOTTING

For co-immunoprecipitation assays with full length ZO-1 and Gγ13, 4 µg of a pcDNA3-FLAG-Gγ13 construct (generous gift of B. Malnic) were co-transfected into HEK 293 cells (60 mm dish) using Lipofectamine LTX (Invitrogen, USA) together with 4 µg of either pcDNA3, full-length pCB6-MYC-ZO-1 or a truncated pCB6-MYC-ZO-1 lacking the PDZ1 domain (pCB6-MYC-ZO-1mut) (generous gift of A. Fanning). pcDNA3-FLAG-Gγ13 + pCB6-MYC-ZO-1 or pcDNA3-FLAG-Gγ13 + pCB6-MYC-ZO-1mut transfections were performed in parallel. Two days later the transfected cells were lysed on ice in 600 µl lysis buffer containing 20 mM Tris, pH 8.0, 150 mM NaCl, 2 mM EDTA, 1% Triton X-100, 0.05% SDS, 1 mg/ml bovine serum albumin, 1 mM DTT and Complete protease inhibitor cocktail (Roche, Switzerland). The lysates were incubated 20 min on ice, centrifuged at 14,000 rpm in a microcentrifuge for 20 min at 4°C and the supernatant incubated overnight at 4°C with 5 µg of mouse monoclonal anti-FLAG (Kodak, USA). Antibody/proteins complexes were recovered with 50 µl protein G-coupled dynabeads (Invitrogen, USA) according to manufacturer's instructions. After three consecutive washes in PBS buffer containing Ca²⁺ and Mg²⁺ the samples were eluted by heating to 80°C for 10 min in LDS sample buffer (Invitrogen, USA) and subjected to SDS-PAGE and Western blot analysis.

For co-immunoprecipitation assays with full length Gγ13 and truncated forms of ZO-1, 3.5 µg of a pDisplay-HA-Gγ13 construct was co-transfected into HEK 293 cells plated on 60 mm dishes using Lipofectamine LTX (Invitrogen, USA) together with 3.5 µg of either pDipslay or various truncated forms of ZO-1, Veli-2, or PSD95 into pDisplay-FLAG. Forty-eight hours later cells were lysed on ice in 600 µl lysis buffer containing 25 mM Hepes, pH 7.5, 5 mM MgCl₂, 4 mM EDTA, 1% Triton X-100, and Complete protease inhibitor cocktail (Roche, Switzerland). Protein extracts were treated essentially as described above except that 8 µg 12CA5 mouse monoclonal anti-HA antibody (Roche, Switzerland) were used for immunoprecipitation.

For Western blotting, IP products or total protein lysates (30 µg) were typically separated on a denaturing 4–12% Bis-Tris PAGE gel (Invitrogen, USA), transferred onto a hybond-P, PVDF membrane (GE Healthcare, USA) and incubated overnight at 4°C with the appropriate primary antibody. Mouse monoclonal

anti-HA (1/400; Roche, USA) or anti-FLAG (1/1000; Kodak, USA) or rabbit polyclonal anti-Ezrin H-276 (1/500; Santa Cruz, USA) or mouse monoclonal anti-myc tag 9B11 (1/1000; Cell Signaling Technology, USA). The membrane was subsequently processed using the SNAP id system (Millipore, USA) and signal was detected with an HRP-coupled secondary antibody and a chemiluminescent substrate (Supersignal West Pico, Pierce, USA) on a Chemidoc imager (Biorad, USA). Quantification and normalization was performed using ImageLab (Biorad, USA). When necessary membranes were stripped using a stripping solution (Uptima, USA) and reprobed with another primary antibody.

To analyze the expression of the PDZ domain-containing proteins and test the specificity of the antibodies used for immunohistochemistry circumvallate papillae and whole olfactory epithelia of fifteen 6–8 weeks old C57BI/6J mice were collected and pooled together. Tissue lysates were prepared in lysis buffer using a tissue lyser (Qiagen, Germany) during three cycles of 90 s each at 20 Hz. After centrifugation the soluble fraction was recovered and the protein content assessed. Seventy-five microgram of each lysate were separated on denaturing 4–12% Bis-Tris PAGE gel (Invitrogen, USA), transferred onto a hybond-P, PVDF membrane (GE Healthcare, USA) and incubated overnight at 4°C with the appropriate primary antibody. Mouse monoclonal anti-β-actin (1/1000; A5441; Sigma, USA), or rabbit polyclonal anti-GOPC (1/500; SAB3500332, Sigma, USA), or rabbit polyclonal anti-ZO-1 (1/600; 40–2200; Invitrogen, USA), or mouse monoclonal anti-MPDZ (1/250; 611558; BD Tranduction Laboratories, USA), or goat polyclonal anti-Gγ13 (1/200; sc-26781; Santa Cruz Biotechnology, USA). The membrane was subsequently processed as described above. Comparison of the expression levels of ZO-1 and Gγ13 in postnatal and adult mice was carried out by collecting olfactory epithelia from 6 P0, 3 P30, and 15 adult animals, pooling the samples from the animals of the same age and preparing tissue lysates as described above. 75, 100, and 130 µg of each extract were separated on a 4–12% Bis-Tris PAGE gel and transferred onto hypond-P. Each membranes which contained samples from either P0 and adult or P30 and adult animals were immunoblotted with a rabbit polyclonal anti ZO-1 mid (1/500; 40–2200; Invitrogen, USA) or a mix of goat polyclonal anti-Gγ13 (1/200 sc-26781 + sc-26782; Santa Cruz Biotechnology, USA) and immunoreactivity evaluated by densitometry (ImageLab; Biorad, USA). The signal intensity for each protein load was expressed as the percentage of the younger animal to the adult and the median value determined.

## IMMUNOHISTOCHEMISTRY

Immunostaining of taste tissue: C57BI/6J mice deeply anesthetized by intraperitoneal injections of sodium pentobarbital (60 mg/kg) were perfused with 4% paraformaldehyde (PFA). Following perfusion the tongue was removed and circumvalate papillae were excised and soaked 2 h in 4% PFA at 4°C before soaking overnight in 20% sucrose at 4°C. The next day the tissue was snap frozen in isopentane chilled with liquid nitrogen and embedded in OCT medium (Tissue-Tek, Japan) before performing sections (16 µm) on a Leica CM3050S cryostat (Leica Microsystems, Germany). Sections were air dried for 2 h at room temperature, and stored at –80°C. The day of
experiment sections were rehydrated in 0.1 M phosphate saline buffer (PBS, pH 7.4) for 10 min and blocked in 5% goat serum, 0.2% Triton X-100 in PBS for 30 min at room temperature before overnight incubation at 4°C with a 1/100 dilution of the appropriate primary antibodies. Commercial antibodies used were: an affinity purified goat polyclonal anti-Gy13 (sc-26781; Santa Cruz Biotechnology, USA). This antibody was raised against an N-terminal peptide of human Gy13 and has been validated previously on mouse taste tissue (Ohtubo and Yoshii, 2011). Immunoblotting shows that it recognizes Gy13 and does not cross-react with ZO-1 in HEK 293 cells co-expressing both proteins (not shown). A mouse monoclonal anti-β-actin (A5441; Sigma, USA), these ascites recognize a single protein of the expected molecular weight in immunoblotting applications (see Figure 2). This antibody has been previously used to stain taste buds in rodents (Hofer and Drenckhahn, 1999). An affinity purified rabbit polyclonal anti-GOPC (SAB3500332; Sigma, USA) raised against a 16 amino acid peptide from near the carboxy terminus of human PIST. The specificity of this antibody was tested by the manufacturer. The specificity of this antibody and its restricted staining pattern in mouse taste buds was previously reported (Michlig et al., 2007). A rat monoclonal anti-ZO-1 (MAB1520; Chemicon International, USA). Two rabbit polyclonal anti-ZO-1 (Invitrogen, USA) one raised against amino acids 463–1109 of a human recombinant ZO-1 fusion protein (Cat # 61–7300); the other raised against a synthetic peptide of the mid region of human ZO-1 (Cat # 40–2200). The latter two antibodies recognized a ZO-1 myc tagged protein over-expressed in HEK 293T cells by western blot. Furthermore these antibodies did not cross-react with Gy13 (not shown).

The next day sections were washed repeatedly and incubated for 2 h at room temperature with the appropriate combination of labeled secondary antibodies (1/500 dilution of Alexa 564-conjugated donkey anti-goat IgG (Molecular Probes, USA), 1/500 dilution of Alexa-488-conjugated donkey anti-rabbit IgG (Molecular Probes, USA). Staining specificity was assessed by treating slices in the absence of primary antibodies. After washing and counterstaining with Hoechst 33342 reagent (Sigma, USA), slides were mounted in gel/mount (Biomeda Corp., USA) and analyzed under a TCS-SP2 confocal microscope (Leica, Germany).

Immunostaining of the olfactory epithelium (OE): C57BI/6J mice were deeply anesthetized by injection of ketamine HCl and xylazine (150 mg/kg and 15 mg/kg body weight, respectively) and then decapitated. The nasal septum was dissected out, the OE removed and subsequently immersed in cold oxygenated ACSF containing in mM: NaCl, 124; CaCl₂ 2; NaH₂PO₄ 1.25; MgSO₄ 1.3; glucose 15, and NaHCO₃ 26, respectively. Mice olfactory epithelia were then fixed using PFA 4% in PBS containing 0.2% glutaraldehyde. Epithelia were further dipped in 95% ethanol for 1 min, washed three times with PBS, blocked with 2% donkey serum, 2% BSA, and 0.25% Triton X-100 in PBS for 1 h and incubated overnight at 4°C with the primary antibodies diluted in blocking solution. Primary rabbit anti-ZO-1 and goat anti-Gy13 (Santa-Cruz SC-26781) were used at 1:100. Sections were washed three times in PBS and incubated with the secondary antibodies (1:500, goat Alexa488-anti-rabbit, donkey

Alexa488 anti-goat or donkey Alexa555 anti-rabbit, Molecular Probes, Invitrogen) in blocking solution for 2 h at room temperature. Epithelia were mounted between slide and coverslip using MOWIOL 4-88 (Merck, Germany). All Chemicals were from Sigma unless stated.

Samples were visualized using a Leica SP2 laser scanning confocal microscope and a 63× oil immersion objective. Ciliary length was used as an indicator of neuron maturity (see Schwarzenbacher et al., 2005) and for each knob the lengths of all the cilia were averaged. Results are given as mean ± SEM, *n* = number of olfactory knobs.

### RT-PCR

To analyze the expression of the PDZ domain-containing genes in various tissues 6-weeks-old C57BI/6J mice were killed with an overdose of sodium pentobarbital, and circumvallate papillae, whole olfactory epithelia (including nasal respiratory epithelium), tongue epithelium devoid of taste buds, liver, and whole brain were dissected out of the carcass on ice. Total RNA extraction, cDNA synthesis and PCR amplification were performed as previously described (Fenech et al., 2009). Primer pairs sequences and annealing temperature are listed in Table A1.

## RESULTS

### Gy13 PHYSICALLY INTERACTS WITH ZO-1, GOPC, AND MPDZ

Earlier work reported an interaction between the C-terminus PDZ binding CTIL motif of Gy13 and the third PDZ domains (PDZ3) of PSD95 or SAP97, or the single PDZ domain of Veli-2 (Li et al., 2006).

To identify additional PDZ-domain containing proteins interacting with Gy13 which might be relevant to taste biology, we conducted a yeast two-hybrid assay using Gy13 as a bait against a selection of five PDZ-domains (Kalyoncu et al., 2010). Some PDZ domains were chosen on the basis of their relative homology to the PDZ3 of PSD95, such as for the multiple PDZ domain protein (MPDZ) PDZ10 and PDZ11 (Figure 1A). We also selected the PDZ domains of GOPC (golgi-associated PDZ- and coiled-coil motif-containing protein) and MPDZ PDZ12 which are related to that of Veli-2. In addition, to broadly screen for a novel interaction we also included more divergent PDZ domains such as those of RGS12 (regulator of G protein signaling 12), MPDZ PDZ13, and PDLIM2 (PDZ and Lim domain protein 2). Finally, the three PDZ domains of ZO-1, a tight junction protein belonging to the MAGUK protein family, were also incorporated. MAGUK proteins typically contain multiple PDZ domains and a GUK domain; PSD95 and SAP97 belong to that family.

Plasmids containing either the entire coding sequence of the mouse Gy13 (pBait) or each of the PDZ domain sequences listed above (pPrey) were co-transformed into competent yeast cells and plated out on selective growth media. During an initial screen we uncovered robust interactions with the PDZ1 of ZO-1, the PDZ domain of GOPC and the PDZ12-13 of MPDZ. In contrast, the PDZ domains of RGS12, PDLIM2, PDZ2, and 3 of ZO-1 as well as PDZ10-11 of MPDZ showed weak or no interaction under those conditions (Figure 1B and Table A2). Note that the PDZ3 of PSD95 which we used as a positive control displayed a relatively weak interaction under these conditions.
<figure><img src="image_3.png" /></figure>

**FIGURE 1** | Gy13 interacts with the PDZ domains of GOPC, MPDZ and ZO-1. (A) Phylogenetic tree of a selection of PDZ domains. Sequences encompassing the PDZ domain region of several proteins were analyzed with clustalW 2.1. using the PAM weight matrix. The PDZ domains presenting the highest homology are closer together on the tree. (*) PDZ domains interacting with Gy13. (B) Individual constructs encompassing each of the ZO-1 PDZ domains (PDZ1, PDZ2, PDZ3), PDZ10-11, and 12–13 of MPDZ, PDZ3 of PSD95 or the unique PDZ domains of PDLIM2, GOPC, and RGS12 (see key) were co-transformed together with Gy13 into MaV203 competent yeast cells and assayed for growth on medium lacking His, Leu, and Trp supplemented with 0 (control plate) or 25 mM 3-AT. ZO-1 (PDZ1), GOPC, and MPDZ (PDZ12-13) are clearly interacting with Gy13. C1 and C2 are

weak- and moderate-strength interaction controls respectively provided by the manufacturer. The results shown are representative of three independent experiments each performed in duplicate. (C) Yeast two-hybrid interaction assay testing the interaction of ZO-1, GOPC, and MPDZ with a mutant Gy13 (T56A) (γ13*). MaV203 competent yeast cells were co-transfected with either the ZO-1 (PDZ1) or GOPC or MPDZ (PDZ12-13) constructs and γ13* and assayed for growth on medium lacking His, Leu, and Trp supplemented with 0 (control plate) or 12.5 mM 3-AT. The T65A mutation clearly abrogates the interaction with these PDZ domains indicating that the c-terminal CTAL motif is critical for this interaction. The results shown are representative of three independent experiments each performed in duplicate.

It was previously reported that the PDZ binding domain of Gy13 is selective for some but not all PDZ domains within the multi-PDZ domain proteins PSD95 and SAP97 (Li et al., 2006). Our results extend this observation to two additional multi-PDZ domain proteins, namely ZO-1 and MPDZ as well as to the mono-PDZ domain protein GOPC. In the case of ZO-1, the first PDZ domain showed the strongest interaction with Gy13, the second PDZ domain interacted very weakly while the third did not interact at all under our experimental conditions. The interaction with MPDZ was also selective for certain PDZ domains since Gy13 appeared more tightly bound to PDZ12-13 than to PDZ10-11 (Figure 1B). When relating these results to the sequence conservation between these PDZ domains (Figure 1A) it appears that the PDZ

domains most similar to Veli-2 such as GOPC and MPDZ (PDZ12) show a strong affinity for Gy13 whereas the divergent RGS12, PDLIM2, and ZO-1 (PDZ2) are very weak interactors.

## Gy13 INTERACTS WITH ZO-1 PDZ1 THROUGH A CLASSIC PDZ BINDING MOTIF—PDZ DOMAIN INTERACTION

It is well known that the residue in position -2 in the canonical X(S/T)XA PDZ binding motif, where X is any amino acid and A any hydrophobic amino acid, is critical for the interaction with type I PDZ domains (Bezprozvanny and Maximov, 2001). To confirm the importance of the CTIL motif of Gy13 in the interaction with ZO-1 PDZ1, GOPC, and MPDZ PDZ12-13 we substituted the threonine in position -2 with
an alanine and subsequently tested the ability of the result-
ing Gγ13T65A mutant to interact with these PDZ domains in
a yeast two-hybrid assay. As shown in **Figure 1C** and as pre-
dicted, the T65A substitution led to a dramatic reduction in the
ability of these proteins to interact together. This result sup-
ports the notion that Gγ13 interacts with these PDZ domains
through a classic PDZ binding motif—PDZ domain type inter-
action (**Table A2**) as previously shown for PSD95 and Veli-2
(Li et al., 2006).

Taken together these results establish for the first time to our
knowledge that Gγ13 binds selectively to MDPZ PDZ12, GOPC,
and ZO-1 PDZ1 via its c-terminal PDZ binding motif.

EXPRESSION OF Gγ13 BINDING PARTNERS

To address whether these newly identified PDZ-containing Gγ13
binding partners were expressed in taste tissue and therefore likely
to be biologically relevant, we carried out a series of related
analyses to look for gene expression and protein content in cir-
cumvallate papillae (CV), a site where both Gγ13 and bitter taste
receptors are abundant (Huang et al., 1999; Matsunami et al.,
2000). First we carried out an RT-PCR experiment to look for
the expression of the genes coding for GOPC, MPDZ, and ZO-1
in CV, surrounding non-sensory tongue tissue, whole OE, whole
brain and liver. Since many splice variants of MPDZ have been
reported previously, for this gene we designed primers flanking
the 12–13 PDZ domains pair to specifically confirm their expres-
sion in CV. In addition, to monitor the presence of OSNs in
our OE sample we used specific primers against Gγ13 while spe-
cific primers against Gøgust, a G-protein alpha subunit selectively
expressed in a subset of TRCs, allowed us to probe their pres-
ence in our CV sample. Glutaraldehyde phosphate dehydrogenase
(GAPDH) amplification and a reaction that does not contain
reverse transcriptase were carried out as controls to validate the
quality of the cDNA reaction and specificity of primer pairs used.
Our results show (Figure 2A) that ZO-1, GOPC, and MDPZ are
broadly expressed and therefore detected in all tissues tested. In
contrast Gγ13 and Gøgust's expression appear restricted to CV
and OE samples despite reports of their expression in certain
brain cells. We believe that too great of a dilution of the mRNAs
for these genes in our whole brain extracts is the reason for
the absence of detection in this tissue under our amplification
conditions (25 PCR cycles).

To investigate further the localization of the Gγ13 interact-
ing proteins in taste bud cells we prepared sections of CV taste
buds which were incubated with antibodies raised against MPDZ,
GOPC, or ZO-1. Prior to immunohistochemical staining the
specificity of the antibodies was verified using immunoblots con-
taining protein extracts from murine CV and OE as well as from
HEK 293 cells untransfected or co-transfected with ZO-1 and
Gγ13 expression constructs. Antibodies raised against MPDZ,
GOPC, ZO-1, and Gγ13 revealed bands of the expected molec-
ular weight in CV, OE, untransfected and ZO-1/Gγ13 transfected
HEK 293 cells (Figure 2B) thus corroborating the gene expression
data obtained by RT-PCR (Figure 2A). The presence of additional
bands detected by the anti-ZO-1 (in CV, OE, and HEK 293) and
anti-MPDZ antibodies in HEK 293 cells is likely linked to the
presence of splice variants of these proteins in these cells/tissues.

We noted that the Gγ13 protein was of higher molecular weight
in CV as compared to OE. Alternative splicing is unlikely to be
the reason behind this higher molecular weight since the RT-PCR
product generated with primers encompassing the entire coding
region of Gγ13 is of the expected size in CV and OE (Figure 2A).
Additional investigations using another antibody directed against
an epitope in the middle of the Gγ13 coding sequence points
toward a post-translational modification preventing binding of
the antibody at this site as the higher molecular weight band was
not revealed in CV (Figure A1). Although, GOPC was detected
both in CV and OE it was ~4 fold more abundant in the latter
(Figure 2B).

Next, we sought to establish whether these proteins were con-
fined to taste bud cells as it is the case for Gγ13. Immunostaining
of CV sections with the anti-MPDZ antibody revealed the pres-
ence of immunopositive taste bud cells (Figure 2C). MPDZ was
detected mainly in the cytoplasm with a small fraction near the
pore.

Gγ13 was confined to a subset (~20%) of taste bud cells,
presumably type II cells, and although distributed throughout
these cells it was most abundant in the cytoplasm as previously
reported. Similarly GOPC was confined to a subset of taste bud
cells and its subcellular distribution appeared restricted to
the cytoplasm and somewhat near the peripheral plasma membrane
(Figure 2C).

In contrast, immunostaining with the antibody raised against
ZO-1 pointed to a different sub-cellular distribution with most
of the protein localized at the taste pore (Figure 2C). This distri-
bution is consistent with the location of tight junctions in these
cells.

Because of the proximal location of ZO-1 to the microvilli
where Gγ13 is thought to operate downstream of T2Rs and its
role in paracellular permeability paramount to taste cell function,
we decided to focus subsequent experiments on the study of the
interaction between Gγ13 and ZO-1.

SELECTIVITY AND STRENGTH OF THE INTERACTION BETWEEN
Gy13 AND ZO-1

In the next set of experiments, we sought to examine the strength
of the interaction between Gγ13 with ZO-1 in a more quanti-
tative way. To this end we took advantage of the fact that with
the ProQuest yeast two-hybrid system the level of expression of
the HIS3 reporter gene is directly proportional to the strength
of the interaction between the two assayed proteins. To grade
the strength of the interaction between the proteins tested, yeast
clones were plated on selection plates lacking histidine and con-
taining increasing concentrations of 3-AT, an HIS3 inhibitor.
Yeast clones containing Gγ13 and ZO-1 (PDZ1-2) grew on selec-
tion plates containing up to 50 mM of 3-AT (Figure 3A). This
clearly demonstrates a strong interaction between these proteins.
The strength of this interaction is only slightly less robust than
that observed with claudin-8 a four-transmembrane domain pro-
tein integral to taste bud tight junctions previously reported to
interact with the PDZ1 of ZO-1 via its c-terminal PDZ binding
domain (Itoh et al., 1999; Michlig et al., 2007). No interac-
tion was observed between claudin-8 and ZO-1 (PDZ2-3) as
expected; however, Gγ13 associated weakly with ZO-1 (PDZ2-3)
<figure><img src="image_4.png" /></figure>

**FIGURE 2** | Expression of GOPC, MPDZ, ZO-1 and Gγ13 in circumvallate papillae. (A) RT-PCR experiment demonstrating expression of ZO-1, GOPC, and MPDZ in all tissues tested. In contrast, the presence of Gγ13 and Gαgust (GUST) mRNAs appear to be restricted to taste and olfactory sensory tissues. See Section "RT-PCR" and **Table A1** for details about amplification conditions and expected sizes of PCR products. GAPDH primers were used as a control of the quality of the RNA. (+) and (−) indicate the presence or absence of reverse transcriptase in the reaction respectively. L: 100 bp ladder. NT, tongue epithelium deprived of taste buds; CV, circumvallate papillae; OE, olfactory epithelium; Br, whole brain; Liv, liver. The results presented are representative of three independent experiments. (B) Immunodetection of ZO-1, MPDZ, GOPC, and Gγ13 proteins in circumvallate (CV) and whole olfactory epithelium (OE) protein extracts. Sample preparation and immunodetection conditions following western blotting are as described in detail under Section "Co-immunoprecipitation and Western blotting." Protein extracts from HEK 293 cells (HEK) or HEK 293 cells stably expressing HA-Gγ13 transiently transfected with a full length Myc-ZO-1 construct (HEK ZO-1 γ13) were used as controls. As expected ZO-1 and Gγ13 are detected in CV and OE. Note that Gγ13 displays a higher apparent molecular

weight in CV than in OE. Predicted molecular masses for ZO-1, MPDZ, GOPC, Gγ13, and β-actin are 220, 220, 51, 8, and 42 kDa respectively. β-actin was used as loading control. The results presented are representative of three independent experiments. (C) Localization of MPDZ, GOPC, Gγ13, and ZO-1 proteins in circumvallate taste buds sections. Indirect immunofluorescence on longitudinal cryosections of circumvallate papillae was performed as described under Section "Immunohistochemistry." MPDZ, GOPC, and Gγ13 are mainly distributed in the cytoplasm of a subset of taste bud cells while ZO-1 localizes mostly at the taste pore. On the Nomarski image a white dashed line highlights the size and location of one taste bud. Gγ13, MPDZ, and GOPC images are strict confocal optical sections (pinhole 82 µm, airy disk 1) while a wider pinhole was used for the ZO-1 image (pinhole 124 µm, airy disk 1.7), Scale bar = 50 µm. Staining patterns are representative of two independent experiments performed on multiple sections from at least two mice. (D) Drawing representing a longitudinal section of the circumvallate papillae as in (C), showing the location of the taste buds along the walls of the trench under the surface of the tongue. Taste bud cells protrude into the trench through the taste pore.
<figure><img src="image_5.png" /></figure>

**FIGURE 3** | **Physical interaction between heterologously expressed Gγ13 and ZO-1.** (A) To test the strength of the interaction between the proteins assayed Mav203 yeast cells were co-transformed with different combinations of bait and prey plasmids (see key). Interaction was scored on minimal medium plates lacking His, Leu, and Trp but containing increasing concentrations of 3-AT (0–50 mM) a competitive inhibitor of the enzyme involved in histidine biosynthesis. Titration of the strength of the interaction is established by growth potential and compared to weak (C1), moderate (C2) and strong (C3) interaction controls provided by the manufacturer. The construct encompassing the first 2 PDZ domains of ZO-1 [ZO (1–2)] interacts with Gγ13 (γ13) to a similar extend as with the c-terminal tail of claudin 8

(Cla 8) a transmembrane cell–cell interaction protein integral to tight junctions. Weaker interactions between Gγ13 and the PDZ2-3 of ZO-1 [ZO (2–3)], the PDZ3 of PSD95 (PSD95), or the unique PDZ domain of Veli-2 (Veli-2) were also observed. Note that no interaction between claudin 8 and ZO (2–3) was visible as expected. The results presented are representative of three independent experiments performed in duplicate. (B) Schematic drawing recapitulating the different domains of ZO-1 tested for their interaction with Gγ13 by two-hybrid interaction assay. At the top simplified representation of the organization of protein domains in ZO-1 showing the PDZ1, PDZ2, PDZ3, SH3, GUK, actin-binding and proline-rich

(Continued)
**FIGURE 3 | Continued**

domains. The span of the constructs tested by two-hybrid are shown
underneath (black line). The ability of the ZO-1 constructs to interact with
Gy13 in presence of 25 mM 3-AT were scored with a (+) when growth was
observed or (-) when there was no growth. An interaction with Gy13 was
detected whenever the construct contained the first PDZ domain of ZO-1.
(C) To ensure that Gy13 could interact with the native ZO-1 protein,
expression constructs encoding tagged full length ZO-1, or Gy13 proteins
were transiently transfected into HEK 293 cells. Protein extracts were
prepared from cells expressing full length MYC-ZO-1 (ZO-1FL, lane 3),
MYC-ZO-1 missing the PDZ1 domain (ZO-1mut, lane 4), FLAG-Gy13 (lane 5)
or co-expressing FLAG-Gy13 and MYC-ZO-1FL (lane 2), or FLAG-Gy13 and
MYC-ZO-1mut (lane 1) as indicated. Examination of the expression of
MYC-ZO-1 and MYC-ZO-1mut expression by western blot with anti-MYC (WB
myc, second to last panel) revealed that both proteins are produced (~230
and ~208 kDa respectively). Erzin was used as a loading control (WB erzin).
Protein extracts were used to immunoprecipitate the FLAG-Gy13 protein
with an anti-FLAG antibody (IP FG, WB FG). Analysis of the content of the
immunoprecipitated complex (IP FG) using an anti-myc antibody (WB myc)
confirms the interaction of the ZO-1FL or ZO-1mut proteins with Gy13 in the

samples co-expressing ZO-1FL or ZO-1mut and FLAG-Gy13 (lane 1 and 2).
Two additional experiments yielded the same results. (D) To validate the
interactions uncovered using the yeast two-hybrid interaction assay and in
particular the protein domains of ZO-1 important for the interaction with
Gy13, co-immunoprecipitation experiments were performed in HEK 293 cells
following heterologous co-expression of HA-Gy13 with various FLAG-ZO-1
deletion constructs. Cells were left untransfected (lane 1) or transiently
transfected with HA-Gy13 alone (lane 6) or in combination with
FLAG-ZO-1(PDZ2-3) (lane 2), FLAG-ZO-1(PDZ1-2) (lane 3), FLAG-Veli-2(PDZ)
(lane 4), or FLAG-PSD95(PDZ3) (lane 5) as indicated. Protein extracts from
transfected cells were first analyzed for expression of the FLAG-tagged
deletion constructs by western blot using an anti-FLAG antibody (WB FG,
bottom panel). Then anti-HA immunoprecipitation was carried out and
complexes were analyzed by western blot using an anti-FLAG antibody (IP
HA, WB FG, top panel). FLAG-PSD95 and FLAG-ZO-1(PDZ1-2) are detected
(arrowheads) indicating that these domains interact with Gy13 under these
conditions. Anti-HA western analysis of the samples confirms correct
immunoprecipitation of HA-Gy13 (IP HA, WB HA, middle panel). (*) IgG light
chains. The experiment shown is representative of 3 independent
experiments.

presumably through a direct interaction with the second PDZ
domain of ZO-1 (see **Figure 1B**).

INTERACTION OF Gγ13 AND ZO-1 IN HEK 293T CELLS

To validate our yeast two-hybrid assay interaction results between ZO-1 and Gγ13 we next tested whether these proteins would co-immunoprecipitate when co-expressed in HEK 293 cells. In order to rule out the possibility that folding of the native protein would prevent this interaction, full-length ZO-1 and Gγ13 constructs were used for this experiment. HEK 293 cell lines stably expressing a MYC-ZO-1 or a MYC-ZO-1 mutant lacking the PDZ1 domain (generous gift of A. Fanning) (Fanning et al., 1998) were transiently transfected with a FLAG-Gγ13 (generous gift of B. Malnic) (Kerr et al., 2008) construct. Forty-eight hours later protein extracts from these cells were prepared and used for immunoprecipitation using an anti-FLAG antibody. Western blot analysis of simple protein extracts from transfected cells using anti-MYC and anti-FLAG antibodies confirms that all full length and mutant proteins are produced in these cells (Figure 3B). Immunoprecipitation of Gγ13 using an anti-FLAG antibody pulled down both intact MYC-ZO-1 and mutant constructs thus supporting further our contention that Gγ13 and ZO-1 physically interact. The interaction of the MYC-ZO-1 mutant construct with Gγ13 despite the absence of the PDZ1 domain can potentially be explained by the fact that as shown in Figures 1B and 3A Gγ13 interacts weakly with the PDZ2 of ZO-1 in yeast cells. Alternatively, it is possible that the transfected MYC-ZO-1 mutant binds the endogenous ZO-1 (see Figure 2B) through an already documented PDZ2 mediated interaction (Utepbergenov et al., 2006). This homodimer would allow Gγ13 to be pulled down along with the MYC-ZO-1 mutant through an interaction with the ZO-1 PDZ1 of the endogenous ZO-1.

In order to further investigate these two possibilities we gener-
ated two truncated FLAG-tagged ZO-1 constructs encompassing
either the first and second (PDZ1-2) or the second and third
(PDZ2-3) PDZ domains of ZO-1 as well as a Gγ13 construct

harboring an HA tag at the N-terminal. We also made FLAG-PSD95 (PDZ3), and FLAG-Veli-2 (PDZ) control constructs. The HA-Gγ13, along with each FLAG-tagged construct were transfected in HEK 293 cells. Forty-eight hours after transfection the cell lysates were subjected to immunoprecipitation with an anti-HA antibody. Lysates from untransfected cells and cells transfected with the HA-Gγ13 construct alone were used as controls. Analysis of the immunoprecipitates by immunoblotting using an anti-FLAG antibody showed that Gγ13 co-precipitated with ZO-1 (PDZ1-2) and PSD95 (PDZ3) but not with ZO-1 (PDZ2-3) or Veli-2 (PDZ) (Figure 3C). Analysis of the HEK 293 cell lysates by immunoblot using an anti-FLAG antibody indicates that all the FLAG-tagged constructs including ZO-1 (PDZ2-3) and Veli-2 (PDZ) were produced and therefore available for co-immunoprecipitation. These results corroborate our yeast two-hybrid assay results (Figures 1B and 3A) and effectively rule out the possibility that binding of Gγ13 to the second PDZ domain of ZO-1 is strong enough to withstand the harsh conditions of this assay. We also note that under these conditions the weak interaction between Gγ13 and Veli-2 is not recapitulated.

Yeast two-hybrid and co-immunoprecipitation assay data strongly supporting a direct interaction between the c-terminal 4 amino-acids of Gγ13 and the first PDZ domain of ZO-1 are recapitulated in **Figure 3D**.

PARTIAL CO-LOCALIZATION OF MPDZ, GOPC, OR ZO-1 WITH
Gy13 IN MOUSE TASTE BUD CELLS

In circumvallate taste buds Gγ13's expression is restricted to type
II cells where it is thought to play a role in bitter taste signal
transduction (Huang et al., 1999; Clapp et al., 2001). In addi-
tion, immunohistochemical analysis of circumvallate, fungiform
or soft palate papillae indicates that Gγ13 is particularly abun-
dant in the cytoplasm of these cells (Clapp et al., 2001; Ohtubo
and Yoshii, 2011). To test whether MPDZ, GOPC, and ZO-1
are co-localized with Gγ13 in mouse taste bud cells, circumval-
late papillae were dissected out and double- immunofluorescent
labeling experiments on sagittal cryosections were performed.
Optical sections of tissue were acquired under a confocal micro-
scope focusing on the region of interest and overlayed with the
software.

Analysis of tissue sections co-stained with Gγ13 (Figure 4A) and MPDZ (Figure 4C) focusing on confocal optical sections near the pore shows that a small fraction of the Gγ13 staining overlaps with that of MPDZ in that area (Figure 4B). On tissue sections double labeled with GOPC (Figure 4D) and Gγ13 (Figure 4F) analysis of single optical sections through the cytoplasm of taste bud cells where Gγ13 is abundant, revealed an extensive co-localization with GOPC at that location (Figure 4E). In addition, a similar partial co-localization pattern between ZO-1 (Figure 4G) and Gγ13 (Figure 4I) was observed on single optical sections through the taste pore (Figure 4H). This pattern was further confirmed using two additional antibodies raised in a different host and targeting different epitopes in ZO-1 (data not shown). Partial co-localization between MPDZ, GOPC, or ZO-1, and Gγ13 in taste bud cells indicates that these proteins might be involved in a dynamic process within the cell and supports the claim that they are likely biological partners.

These experiments also revealed that all TRCs expressing
Gγ13 are immunopositive for GOPC, further emphasizing a tight
collaboration between these two proteins. GOPC immunoreac-
tivity was observed as well in cells that did not express Gγ13
(Figure 4E), presumably in type I or III cells. Unfortunately the
rather weak immunostaining with the MPDZ specific antibody
and the very restricted location of ZO-1 around the tight junc-
tions prevented an in depth study of the cell types expressing these
proteins.

CO-LOCALIZATION OF ZO-1 AND Gγ13 IN OLFACTORY SENSORY NEURONS

Both ZO-1 and Gγ13 have been independently reported to be expressed in OSNs (Miragall et al., 1994; Kulaga et al., 2004). In order to investigate whether Gγ13 and ZO-1 co-localize in olfactory neurons, we set-up a flat-mount (or << en face >>) preparation of OE allowing us to image individual olfactory neuron dendritic knobs. First, in P30 mice no co-localization between Gγ13 and ZO-1 was ever seen in Gγ13 immunopositive knobs (n = 220, **Figure 5A**). Next, we analyzed newborn mice (P0). At this stage dendritic knobs could be split into two groups (Schwarzenbacher et al., 2005). A first group did not display any cilia and was recognizable by its round smooth aspect (**Figure 5B**). In this group co-localization was found in 66.6% of the dendritic knobs (n = 9 knobs). In a second more important group encompassing dendritic knobs bearing small ciliary compartments (**Figure 5C**) co-localization between Gγ13 and ZO-1 was seen in 73% of the ciliated dendritic knobs (n = 27 knobs). Overall co-localization could be observed in 72.2% of the Gγ13 immunopositive dendritic knobs (n = 36) at P0. Finally and in line with these observations, dendritic knobs where co-localization between the two proteins was seen had shorter cilia (average length per knobs 2.8 ± 0.2 mm, n = 20) compared to the ones where no co-localization was observed (n = 5.5 ± 1.0 mm, n = 7, p < 0.01 Mann-Whitney). We, therefore, infer that co-localization between Gγ13 and ZO-1 depends upon the developmental stage of olfactory neurons. Note that

the secondary antibody alone did not produce any background
staining (Figure 5D).

Next ZO-1 and Gγ13's protein expression levels in olfactory mucosa were evaluated during development by western blot. The signal intensity expressed as the percentage of the younger animal to the adult indicates that there is a slight decrease of ZO-1 expression from 84% at P0 to 63% at P30 while Gγ13's expression increased from 15% at P0 to 33% at P30. Given these results we cannot completely rule out the possibility that the lack of co-localization observed at P30 might be linked with the slight decrease of ZO-1 expression at this stage.

DISCUSSION

A NETWORK OF PROTEINS POTENTIALLY REGULATING THE
INTRACELLULAR TRAFFIC OF Gγ13 IN TASTE RECEPTOR CELLS

Following up on an earlier report demonstrating an interaction
between Gγ13 and the PDZ domain containing proteins Veli-2
and SAP97, our data identified GOPC, MPDZ, and ZO-1 as bind-
ing partners of Gγ13. We also report for the first time to our
knowledge the expression of GOPC and MPDZ in taste bud cells.

All three PDZ-containing proteins identified in this study are
known members of macromolecular complexes or participate in
protein trafficking suggesting that they are likely to determine
Gγ13's transport and/or subcellular location in taste cells.

GOPC is a Golgi-associated protein reportedly interacting
with a number of transmembrane proteins including chan-
nels and GPCRs for which it is thought to modulate vesicular
transport from the Golgi apparatus to the plasma membrane. In
addition it is known to associate with the Rho effector Rhotekin
at adherent junctions where it is thought to regulate cell-polarity
development (Ito et al., 2006). These features might explain in
part both the punctate staining pattern as well as the staining
observed at the periphery of the taste bud cells (Figure 2C).
Although, this is the first report of GOPC's expression in TRCs,
this new finding is not totally surprising considering that TRCs
are polarized neuroepithelial sensory cells much like inner ear
sensory hair cells of the cochlea where GOPC regulates membrane
trafficking of cadherin 23 (Xu et al., 2010), a cell-cell adhesion
protein also found in retinal cells where its loss is associated with
retinitis pigmentosa (Bolz et al., 2001). In hair cells GOPC retains
cadherin 23 in trans-golgi networks (TGNs). Co-expression of
MAGI-I and harmonin, two PDZ domain-containing proteins,
competes with GOPC to cause the release of cadherin 23 from
the TGN. It is plausible that in TRCs MPDZ, which we find dis-
tributed in the cytoplasm and to a small extent near the tight
junctions, fulfills the same function as MAGI-I. Under this sce-
nario we would assume that MPDZ is able to compete with
GOPC for Gγ13 binding and once unloaded onto MPDZ, Gγ13
is transported to the taste bud pore. Coincidentally, MPDZ has
been reported to interact with the tight junction complex, par-
ticularly with claudin-1 in polarized epithelial cells; therefore, its
localization at the pore is not completely unexpected (Hamazaki
et al., 2002; Liew et al., 2009). Our own experiments corrob-
orate these findings by showing that although MPDZ seems
most abundant in the cytoplasm of taste bud cells, a fraction
of it is detected at the pore where it is partly co-localized with
ZO-1 (Figure A2).
<figure><img src="image_6.png" /><figcaption>**FIGURE 4** | Partial co-localization of Gγ13 with its interaction partners in mouse taste bud cells. Laser scanning confocal microscope analysis of sagittal sections of circumvallate papillae incubated simultaneously with specific antibodies raised against Gγ13 and either ZO-1, MPDZ, or GOPC and revealed with the appropriate fluorescent secondary antibodies. Each image shows one entire taste bud (apical: up, basal: down). Partial co-localization between Gγ-13 and MPDZ (A–C) is observed in the cytoplasm and to a small extend the pore (white arrows). GOPC and Gγ-13 staining (D–F) shows an</figcaption></figure>

extensive overlap in the cytoplasmic region (yellow arrows) but not near the pore (purple arrow). Partial co-localization of ZO-1 and Gγ-13 (G–I) is evident at the pore where tight junctions are located. The images presented are single optical sections (not stacks) collected under strict confocal conditions (airy disk 1, GOPC/Gγ-13 Pinhole 82 µm, GOPC or ZO-1/Gγ-13 Pinhole 115 µm). Confocal images where merged electronically using Photoshop. Scale bar 15 µm. Images are representative of staining patterns obtained in >6 taste buds from three mice.

Alternatively Veli-2, another cytosolic Gγ13 binding protein might be able to fulfill the same function (Li et al., 2006). It is interesting to note that both MAGI-I and MDPZ have several (>5) PDZ domains suggesting that in addition to Gγ13 they might concomitantly bind additional proteins such as receptors and channels. GABAB receptors which have been detected in TBCs and shown to interact with MPDZ represent such an example (Balasubramanian et al., 2007; Cao et al., 2009). Once at the tight junction, ZO-1 would allow docking of Gγ13 and perhaps regulate its entry into the microvilli. In this regard, it is worth noting that detection of Gγ13 in microvilli of TRCs appears weak compared to what is observed in olfactory cilia suggesting that

entry of Gγ13 in microvilli is tightly regulated. Alternatively, this interaction might affect paracellular permeability as discussed below.

It is conceivable that within the microvilli Gγ13 could travel to the apical tip through an interaction with the PDZ domain containing protein SAP97 as previously suggested (Li et al., 2006). There Gγ13 would become anchored to the plasma membrane following prenylation of its c-terminal cystein residue. This event would signal the end of the road for Gγ13 as prenylation is preceded by the removal of the residues downstream of the cystein thus eliminating the PDZ binding site as previously noted by Li et al. (2006). At its final destination Gγ13 would presumably
<figure><img src="image_7.png" /></figure>

**FIGURE 5** | **Co-localization of ZO-1 and Gγ13 in mouse olfactory sensory neurons is age-dependent.** Series of confocal images showing age-dependent co-localization between Gγ-13 (red) and ZO-1 (green) in mouse olfactory dendritic knobs. (A) In P30 mice the immunostaining for

ZO-1 (blue arrow) does not co-localize with the Gγ-13 immunostaining. (B) In P0 mice a strong co-localization within olfactory dendritic knobs devoid of cilia as well as neurons bearing small-sized cilia (C) is observed. (D) Control experiment performed by omitting the primary antibody. Scale bar 5 µm.

assemble with Gβ1 and Gαgust to participate in signaling downstream of T2R receptors (Huang et al., 1999). Although the exact sequence of events remains to be confirmed we note that the short sequence between the βB and βC regions of the PDZ domains of PSD95 and Veli-2 thought to accommodate the prenyl group of Gγ13 (Li et al., 2006) is absent from ZO-1 (PDZ1) and MPDZ (PDZ12) (Figure A3) perhaps indicating that prenylation occurs later in this sequence.

## Gγ13 AT THE TIGHT JUNCTION

The tight junction of polarized epithelial cells plays a fundamental role in the regulation of the paracellular permeability barrier as well as the maintenance of apical and basolateral compartments. Interestingly, heterotrimeric G protein signaling has been implicated in tight junction biogenesis and permeability regulation. Consistent with this a number of modulators of G protein activity (Alf4, cholera, and pertussis toxins) affect tight junction assembly (Balda et al., 1991) and several G protein α subunits including Gαi2, Gαo, Gα12, and Gαs have been located at the tight

junction (Saha et al., 2001). In fact, it was recently shown that activation of Gα12, which interacts directly with ZO-1 through its SH3 domain, disrupts the tight junction through a c-Src mediated pathway thereby increasing paracellular permeability (Meyer et al., 2002; Sabath et al., 2008). Heterotrimeric G proteins mediate GPCR signaling through Gα and Gβγ subunits and as expected one GPCR has been reported to regulate tight junction permeability in a pertussis-sensitive manner. This is the case of the somatostatin 3 receptor (SSTR3) which is targeted to the tight junction through a direct interaction between a PDZ binding motif in its c-terminal tail and MPDZ PDZ10 (Liew et al., 2009). Finally, another component of the G protein cascade, namely regulator of G protein signaling 5 (RGS5) has also been reported to interact with ZO-1 (Bal et al., 2012).

Although there are no prior reports of Gβγ subunits at the tight junction, our finding that Gγ13 interacts directly with ZO-1 and MPDZ is not totally unexpected. However the role it might play on TJ assembly, maintenance of polarity, or paracellular permeability in taste bud cells remains to be established.
## Gγ13 IN OLFACTORY SENSORY NEURONS

In stark contrast to what is observed in microvilli, Gγ13 is readily detected in cilia of OSNs where it is thought to be involved in sensory signaling. Our observation that Gγ13 and ZO-1 co-localize in the OE of neonates but not in that of adult animals suggests that this interaction might be important during the maturation of the epithelium in mice. In adult rat OE, ZO-1 is localized at apical tight junctions connecting the dendrites of OSNs and surrounding supporting cells (Miragall et al., 1994). Claudins 1, 3, 4, and 5 are part of the apical tight junction complex forming a selective barrier necessary for proper signaling in OSNs (Steinke et al., 2008). Despite the fact that tight junctions in TRCs and OSNs share a number of components including claudin 1, claudin 4, and ZO-1, the absence of co-localization between Gγ13 and ZO-1 in the adult OE clearly points to important organizational dissimilarities in these tissues.

Another notable difference between these tissues includes the fact that in OSNs MPDZ is mainly restricted to the cilia where it is thought to regulate odorant evoked signal duration through a direct interaction with odorant receptors (Dooley et al., 2009). As a result, MPDZ has been deemed a major component of the signalosome downstream of odorant receptors also known as “olfactosome.” Our findings extend this concept by showing that another component of the olfactory signaling cascade abundant in cilia, namely Gγ13, also interacts with MPDZ.

Although, there are no current reports of GOPC in OSNs, here we present data indicating that GOPC is detected in the OE. While its precise location and sub-cellular distribution in the OE remains to be investigated, we suspect that it is involved in retention of Gγ13 in the TGN.

## Gγ13 AND SENSORY SIGNALING

GPCRs couple selectively to Gα subunits which themselves associate selectively with Gβγ subunits. Upon stimulation of the receptor, both Gα- and Gβγ-mediated processes are activated. Determinants effectively governing downstream events include the repertoire of Gα, Gβ, Gγ and cellular effectors present in the cells expressing the receptor in question as well as the selectivity of the interactions between receptor and Gα subunits and that between Gγ/Gβ subunits and cellular effectors.

If we apply this reasoning to TRCs we note that both Gøgust and Gøi2 are present (McLaughlin et al., 1992; Kusakabe et al., 2000), and that functional and biochemical studies indicate that T2Rs are able to couple to and activate both Gøi/o and Gøgust subunits (Ozeck et al., 2004; Sainz et al., 2007). Experiments with gustducin knock-out (KO) animals implicate both Gøgust and additional Gα subunits in bitter transduction as the KO mice retained sensitivity to bitter substances (Wong et al., 1996). Regarding the beta and gamma subunits, both Gβ1 and Gβ3 have been detected in gustducin expressing cells together with Gγ3 and Gγ13 (Huang et al., 1999; Rossler et al., 2000).

Based on these accounts many possible Gα, Gβ, Gγ combinations may mediate bitter detection in mammals. Nevertheless, it is thought that the heterotrimer composed of Gøgust/Gβ3/Gγ13 is the main player. Under this scenario the Gβ3-Gγ13 complex activates phospholipase C-β2 (PLC-β2) or PLC-β3 (Hacker et al., 2008) while Gøgust acts in parallel on local phosphodiesterases

to modulate intracellular cAMP levels. A recent report puts forward an alternative role for Gøgust in taste cells by demonstrating that its constitutive activity maintains low resting cAMP levels thereby regulating the responsiveness of bitter receptor cells (Clapp et al., 2008). This new hypothesis does not take away from the demonstrated central role of PLC-β2 in bitter transduction (Zhang et al., 2003) and the possible involvement of Gγ13 in this process. Nevertheless, a tissue-specific KO model validating the role of Gγ13 in bitter taste transduction *in vivo* is still missing.

Unlike in the taste cells where PLC signaling is paramount to GPCR-mediated tastant detection, in OSNs disruption of the cAMP pathway leads to anosmia (Brunet et al., 1996; Belluscio et al., 1998; Wong et al., 2000). In olfactory cilia Gγ13 co-localizes and is thought to interact with Gβ1 and Gøolf (Kerr et al., 2008). Although, the recombinant Gβ1γ13 dimer appears to be the second most potent activator of PLC-β isoforms after Gβ1γ7 (Poon et al., 2009), the absence of a convincing demonstration of PLC-β expression in OSNs suggests that in these cells Gγ13 might play another role. Kerr et al. reported that Gγ13 interacts with Ric-8B, a guanine nucleotide exchange factor for Gøolf, and hypothesized that by retaining Ric-8B in proximity of Gøolf-GTP, Gγ13 would facilitate re-association of Ric-8B and Gøolf-GDP which ultimately would maximize the efficiency of that pathway.

Our immunostaining experiments suggest that Gγ13 interacts with ZO-1 temporarily during the maturation of the OSN. The impact this interaction might have on sensory signaling or OSN maturation remains to be investigated. Functional maturation is known to occur in OSNs (Lee et al., 2011). This maturation could be correlated with signaling protein trafficking and involve ZO-1 as it was previously implicated in maturation and regeneration in other cell types (Castillon et al., 2002; Kim et al., 2009). Under this scenario it is conceivable that the interaction between ZO-1 and Gγ13 during OSN maturation might induce some functional changes. In this case a tissue-specific Gγ13 KO mouse model will be a valuable tool to help unravel the role of this protein in OSN function *in vivo*.

Finally, in mouse cone and rod bipolar cells Gγ13 appears to be distributed throughout the cells while Gøo is concentrated in dendrites. The co-expression of Gγ13 with Gβ3, Gβ4, and Gøo in ON cone bipolar cells which do not contain PLC-β suggests that it might be involved in yet another signaling pathway in these cells (Huang et al., 2003). In this tissue where ZO-1 expression has been reported as well (Ciolofan et al., 2006), it would be interesting to investigate whether these proteins are partly co-localized.

## CONCLUSION

In the present study, we report the identification of three novel binding partners for Gγ13. In addition, we provide the first evidence of the expression of two of these proteins (GOPC and MPDZ) in taste bud cells. We anticipate that future work addressing the sequence of these interactions with Gγ13 and their temporality will help shed more light on the precise role these proteins play in efficiently targeting Gγ13 to selective subcellular locations.

By comparing the subcellular location of some of these proteins in OSNs and neuroepithelial taste cells, our study points out possible discrepancies in the mechanisms guiding protein traffic
and subcellular localization in these two cell types. These differences might not be surprising given the differences in the origin (neuronal vs. epithelial) and the architecture of neuroepithelial taste cells and OSNs. In particular, we believe that the differential location of MPDZ and Gy13 in OSNs and TRCs reflects different mechanisms at play in both types of sensory cells and provides some clues as to what their function in these cells might be (transport vs. signalosome). Interestingly, MPDZ is thought to act as a scaffolding protein in the spermatozoa, a polarized cell capable of chemotaxis through taste and odorant receptors (Zitranski et al., 2010).

The presence of MPDZ, ZO-1, and Gy13 at the tight junction in TRCs is intriguing and remains to be investigated further. In this context it is interesting to mention that ZO-1 has been demonstrated to associate with F-actin through an actin-binding region located in the C-terminal half of the molecule (Fanning et al., 2002) and that F-actin filaments are major structural components of taste cells microvilli (Takeda et al., 1989).

Finally, we would like to mention that given the expected importance of Gy13 in taste cells signaling, disruption of any of the interactions reported here could have important consequences on taste reception. There is such a precedent in the OE where polymorphisms in CEP290, a protein which cargoes Gy13,

Gøs, and Gβ1 from the base of the cilia toward the tip, have been linked with anosmia (McEwen et al., 2007).

## ACKNOWLEDGMENTS

We would like to thank Dr. A. Fanning (University of North Carolina at Chapel Hill, USA) for providing the Myc-tagged ZO-1 constructs and kindly sharing protocols with us, Dr. O. Keskin (Koç University, Turkey) for help with the classification of PDZ domains. Dr. E. Assémat (IBDML, Marseille) and C. Neophytou (Emergo, Cyprus) for advice on MPDZ and ZO-1 antibodies respectively, Dr. C. Arnould (INRA, Dijon) for help with confocal microscopy and A. Lefranc for help with animal husbandry. We are grateful to Dr. B. Malnic (University of Sao Paolo, Brazil) for the FLAG-Gy13 construct, insightful comments and suggestions throughout, Dr. V. Dionne (Boston University, USA) for critical reading of the manuscript, and Dr. G. Strichartz (Brigham and Women's Hospital, USA) for support. This work was supported by Action Thematique Incitative sur Programme (CNRS) grants to Jean-Pierre Montmayeur and Xavier Grosmaître, Région de Bourgogne and CNRS poste rouge post-doctoral fellowships to Zhenhui Liu and Esmerina Tili respectively, and funds from GOSPEL (IST-2002-507610) to Fabienne Laugerette and Anna Wiencis.

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**Conflict of Interest Statement:** The authors declare that the research was conducted in the absence of any commercial or financial relationships

that could be construed as a potential conflict of interest.

Received: 03 April 2012; paper pending published: 13 April 2012; accepted: 31 May 2012; published online: 21 June 2012.

**Citation:** Liu Z, Fenech C, Cadiou H, Grall S, Tili E, Laugerette F, Wiencis A, Grosmaitre X and Montmayeur J-P (2012) Identification of new binding partners of the chemosensory signaling protein Gy13 expressed in taste and olfactory sensory cells. *Front. Cell. Neurosci.* 6:26. doi: 10.3389/fncel.2012.00026

Copyright © 2012 Liu, Fenech, Cadiou, Grall, Tili, Laugerette, Wiencis, Grosmaitre and Montmayeur. This is an open-access article distributed under the terms of the Creative Commons Attribution Non Commercial License, which permits non-commercial use, distribution, and reproduction in other forums, provided the original authors and source are credited.
APPENDIX

<figure><img src="image_8.png" /></figure>
<figure><img src="image_9.png" /></figure>

**FIGURE A2 |** Partial co-localization between ZO-1 and MPDZ in CV taste bud cells. Immunolocalization of MPDZ (red) and ZO-1 (green) in circumvallate taste buds. Indirect immunofluorescence on sagittal cryosections of circumvallate papillae was performed as described under Section "Immunohistochemistry." Each image shows one entire taste

bud (apical: up, basal: down). Nuclei (blue) were visualized using nuclear stain. MPDZ and ZO-1 staining overlaps partly in the cytoplasm (yellow arrowhead) and at the taste pore (white arrow). The Nomarski image shows the location of the taste bud. Scale bar = 15 µm. Images are representative of staining patterns from at least 5 taste buds.
<figure><img src="image_10.png" /></figure>
<table><caption>Table A1 | List of primer sequences and corresponding annealing temperatures used for PCR experiments.</caption><thead><tr><th>Gene</th><th>Forward primer sequence</th><th>Reverse primer sequence</th><th>Template Acc#</th><th>Ampl. size (bp)</th><th>Tm (°C)</th></tr></thead><tbody><tr><td>Gng13</td><td>CGAGGTCGACTGAGGAGTGGGATGTGCC</td><td>CTTAGCGGCCGCTCATAGGATGGTGCACTT</td><td>AY029485</td><td>200</td><td>56</td></tr><tr><td>Gng13TA</td><td>CGAGGTCGACTGAGGAGTGGGATGTGCC</td><td>CTTAGCGGCCGCTCATAGGATGGCGCACTT</td><td>AY029485</td><td>200</td><td>54</td></tr><tr><td>Tjp1 (PDZ1)</td><td>CGAGGTCGACTGCAATGGAGGAAACAGCTA</td><td>CTTAGCGGCCGCTTAACTTACAGGGATCTGAACTTTC</td><td>BC138028</td><td>317</td><td>58</td></tr><tr><td>Tjp1 (PDZ2)</td><td>CGAGGTCGACTTCCGTGGCCTCCAGTCAG</td><td>CTTAGCGGCCGCTTAAATTTCTGAAATGTCATCTCTTTCC</td><td>BC138028</td><td>353</td><td>58</td></tr><tr><td>Tjp1 (PDZ3)</td><td>CGAGGTCGACTCCGAAACCTGTGTATGCT</td><td>CTTAGCGGCCGCTTAAACGTCCTTCTTCTCTG</td><td>BC138028</td><td>365</td><td>55</td></tr><tr><td>*Tjp1 (PDZ1-2)</td><td>CGAGGTCGACTGCAATGGAGGAAACAGCTA</td><td>CTTAGCGGCCGCTTAAATTTCTGAAATGTCATCTCTTTCC</td><td>BC138028</td><td>842</td><td>58</td></tr><tr><td>Tjp1 (PDZ2-3)</td><td>CGAGGTCGACTTCCGTGGCCTCCAGTCAG</td><td>CTTAGCGGCCGCTTAAACGTCCTTCTTCTCTG</td><td>BC138028</td><td>995</td><td>55</td></tr><tr><td>Tjp1 (PDZ3-SH3)</td><td>CGAGGTCGACTCCGAAACCTGTGTATGCT</td><td>CTTAGCGGCCGCTTAAGCTGGGAACTTAGTTTGAA</td><td>BC138028</td><td>743</td><td>56</td></tr><tr><td>Tjp1 (SH3)</td><td>CGAGGTCGACTGTGACCATACTGGCTCAG</td><td>CTTAGCGGCCGCTTAAGCTGGGAACTTAGTTTGAA</td><td>BC138028</td><td>410</td><td>56</td></tr><tr><td>Tjp1 (PDZ1-SH3)</td><td>CGAGGTCGACTGCAATGGAGGAAACAGCTA</td><td>CTTAGCGGCCGCTTAAGCTGGGAACTTAGTTTGAA</td><td>BC138028</td><td>1862</td><td>56</td></tr><tr><td>Tjp1 (PDZ1-PDZ3)</td><td>CGAGGTCGACTGCAATGGAGGAAACAGCTA</td><td>CTTAGCGGCCGCTTAAACGTCCTTCTTCTCTG</td><td>BC138028</td><td>1484</td><td>55</td></tr><tr><td>Cldn1</td><td>CGAGGTCGACTCGGAAAACAACCTCTTAC</td><td>CTTAGCGGCCGCTCACACATAGTCTTTCCC</td><td>EU076705</td><td>74</td><td>55</td></tr><tr><td>Cldn4</td><td>CGAGGTCGACTCCTCGTAGCAACGACAAG</td><td>CTTAGCGGCCGCTTACACATAGTTGCTGGCGG</td><td>AF087822</td><td>74</td><td>60</td></tr><tr><td>Cldn8</td><td>CGAGGTCGACTACTGAAAGGAGCAACAGTTA</td><td>CTTAGCGGCCGCCTACACATACTGACTTTTG</td><td>AF087826</td><td>113</td><td>56</td></tr><tr><td>F11r</td><td>CGAGGTCGACCAGCCGTGGATACTTTGAAAGAAC</td><td>CTTAGCGGCCGCTCACACCAGGAACGACGA</td><td>BC021876</td><td>119</td><td>60</td></tr><tr><td>Lin7b</td><td>CGAGGTCGACTCCCAGGGTCGTGGAACTA</td><td>CTTAGCGGCCGCTCAAGTGTAGCGCACCACCAG</td><td>AF087694</td><td>251</td><td>62</td></tr><tr><td>Dlg1 (PDZ3)</td><td>CGAGGTCGACTATCACTAGGGAACCTAGA</td><td>CTTAGCGGCCGCTTAACGACTGTACTCTTCGGG</td><td>AY159380</td><td>279</td><td>55</td></tr><tr><td>Dlg4 (PDZ3)</td><td>CGAGGTCGACTAGGCGGATCGTGATCCAT</td><td>CTTAGCGGCCGCTTACTCTTCTGGTTTATACTGAGC</td><td>BC014807</td><td>256</td><td>58</td></tr><tr><td>Mpdz (PDZ10-11)</td><td>CGAGGTCGACTTCCTCCACACCAGCAGTC</td><td>CTTAGCGGCCGCTTAGGGGGAGTGAAAGATGACAG</td><td>BC145117</td><td>682</td><td>62</td></tr><tr><td>*Mpdz (PDZ12-13)</td><td>CGAGGTCGACTGGAATAAATACATCAGAGTCA</td><td>CTTAGCGGCCGCTCAAGAGAGAACCATGAG</td><td>BC145117</td><td>704</td><td>55</td></tr><tr><td>Pdlim2</td><td>CGAGGTCGACTATGGCGTTGACTGTGGATG</td><td>CTTAGCGGCCGCTTATGTTTGGGACCGGTCCAG</td><td>BC024556</td><td>254</td><td>60</td></tr><tr><td>*Gopc</td><td>CGAGGTCGACTATTAGAAAAGTTCTCCTCCTT</td><td>CTTAGCGGCCGCTTAGACTACTTCAAACTCAATC</td><td>AF287894</td><td>250</td><td>54</td></tr><tr><td>Rgs12</td><td>CGAGGTCGACTGTGCGGAGCGTCGAAGTG</td><td>CTTAGCGGCCGCTCACTCAGCAATCACCATGTGCAG</td><td>BC40396</td><td>233</td><td>62</td></tr><tr><td>*Gng13</td><td>ATGGAGGAGTGGGATGT</td><td>TCATAGGATGGTGCACTTG</td><td>AY029485</td><td>203</td><td>54</td></tr><tr><td>*Gnat3</td><td>TCAGGAGGATGCTGAGCG</td><td>ATGCCCTTTCAAAGCAGGC</td><td>BC147841</td><td>361</td><td>56</td></tr><tr><td>*Gapdh</td><td>TGGTCACCAGGGCTGC</td><td>GTGGCAGTGATGGCATG</td><td>GU214026</td><td>501</td><td>59</td></tr></tbody></table>

(*) Primers and annealing temperatures used for RT-PCR only.

(#) Primers and annealing temperatures used for both cloning and RT-PCR experiments.

(Acc#) GenBank accession number.
<table><caption>Table A2 | Table summarizing the results (from at least three independent experiments) of the yeast two-hybrid interaction assays performed with different combinations of baits and preys.</caption><thead><tr><th>Bait</th><th>Prey</th><th>3-AT (12.5 mM)</th><th>3-AT (25 mM)</th><th>3-AT (50 mM)</th></tr></thead><tbody><tr><td>Gy13</td><td>ZO-1 PDZ1</td><td>+</td><td>+</td><td>—</td></tr><tr><td>Gy13</td><td>ZO-1 PDZ2</td><td>+</td><td>—</td><td>—</td></tr><tr><td>Gy13</td><td>ZO-1 PDZ3</td><td>—</td><td>—</td><td>—</td></tr><tr><td>Gy13</td><td>ZO-1 SH3</td><td>+</td><td>—</td><td>—</td></tr><tr><td>Gy13</td><td>ZO-1 PDZ1-2</td><td>+</td><td>+</td><td>+</td></tr><tr><td>Gy13</td><td>ZO-1 PDZ2-3</td><td>+</td><td>—</td><td>—</td></tr><tr><td>Gy13</td><td>ZO-1 PDZ3-SH3</td><td>+</td><td>—</td><td>—</td></tr><tr><td>Gy13</td><td>ZO-1 PDZ1-2-3-SH3</td><td>+</td><td>+</td><td>—</td></tr><tr><td>Gy13</td><td>Veli-2</td><td>+</td><td>—</td><td>—</td></tr><tr><td>Gy13</td><td>PSD95 PDZ3</td><td>+</td><td>—</td><td>—</td></tr><tr><td>Gy13</td><td>SAP97 PDZ3</td><td>+</td><td>—</td><td>—</td></tr><tr><td>Gy13</td><td>PDLIM2</td><td>—</td><td>—</td><td>—</td></tr><tr><td>Gy13</td><td>GOPC</td><td>+</td><td>+</td><td>—</td></tr><tr><td>Gy13</td><td>RGS12</td><td>+</td><td>—</td><td>—</td></tr><tr><td>Gy13</td><td>MPDZ PDZ10-11</td><td>+</td><td>—</td><td>—</td></tr><tr><td>Gy13</td><td>MPDZ PDZ12-13</td><td>+</td><td>+</td><td>—</td></tr><tr><td>Gy13*</td><td>ZO-1 PDZ1</td><td>—</td><td>—</td><td>—</td></tr><tr><td>Gy13*</td><td>ZO-1 PDZ1-2</td><td>—</td><td>—</td><td>—</td></tr><tr><td>Gy13*</td><td>GOPC</td><td>—</td><td>—</td><td>—</td></tr><tr><td>Gy13*</td><td>MPDZ PDZ12-13</td><td>—</td><td>—</td><td>—</td></tr><tr><td>Claudin 1</td><td>ZO-1 PDZ1</td><td>—</td><td>—</td><td>—</td></tr><tr><td>Claudin 1</td><td>ZO-1 PDZ2</td><td>—</td><td>—</td><td>—</td></tr><tr><td>Claudin 1</td><td>ZO-1 PDZ3</td><td>—</td><td>—</td><td>—</td></tr><tr><td>Claudin 1</td><td>ZO-1 PDZ1-2</td><td>+</td><td>—</td><td>—</td></tr><tr><td>Claudin 1</td><td>ZO-1 PDZ3-SH3</td><td>—</td><td>—</td><td>—</td></tr><tr><td>Claudin 1</td><td>MPDZ PDZ8-9</td><td>+</td><td>—</td><td>—</td></tr><tr><td>Claudin 1</td><td>MPDZ PDZ10-11</td><td>+</td><td>—</td><td>—</td></tr><tr><td>Claudin 1</td><td>MPDZ PDZ12-13</td><td>—</td><td>—</td><td>—</td></tr><tr><td>Claudin 4</td><td>ZO-1 PDZ1</td><td>—</td><td>—</td><td>—</td></tr><tr><td>Claudin 4</td><td>ZO-1 PDZ2</td><td>—</td><td>—</td><td>—</td></tr><tr><td>Claudin 4</td><td>ZO-1 PDZ3</td><td>—</td><td>—</td><td>—</td></tr><tr><td>Claudin 4</td><td>ZO-1 PDZ1-2</td><td>+</td><td>+</td><td>—</td></tr><tr><td>Claudin 4</td><td>ZO-1 PDZ3-SH3</td><td>—</td><td>—</td><td>—</td></tr><tr><td>Claudin 4</td><td>MPDZ PDZ8-9</td><td>+</td><td>—</td><td>—</td></tr><tr><td>Claudin 4</td><td>MPDZ PDZ10-11</td><td>+</td><td>—</td><td>—</td></tr><tr><td>Claudin 4</td><td>MPDZ PDZ12-13</td><td>—</td><td>—</td><td>—</td></tr><tr><td>Claudin 8</td><td>ZO-1 PDZ1</td><td>+</td><td>+</td><td>+</td></tr><tr><td>Claudin 8</td><td>ZO-1 PDZ2</td><td>—</td><td>—</td><td>—</td></tr><tr><td>Claudin 8</td><td>ZO-1 PDZ3</td><td>—</td><td>—</td><td>—</td></tr><tr><td>Claudin 8</td><td>ZO-1 PDZ1-2</td><td>+</td><td>+</td><td>+</td></tr><tr><td>Claudin 8</td><td>ZO-1 PDZ3-SH3</td><td>—</td><td>—</td><td>—</td></tr><tr><td>Claudin 8</td><td>MPDZ PDZ8-9</td><td>+</td><td>—</td><td>—</td></tr><tr><td>Claudin 8</td><td>MPDZ PDZ10-11</td><td>+</td><td>—</td><td>—</td></tr><tr><td>Claudin 8</td><td>MPDZ PDZ12-13</td><td>—</td><td>—</td><td>—</td></tr><tr><td>JAM</td><td>ZO-1 PDZ3</td><td>—</td><td>—</td><td>—</td></tr><tr><td>JAM</td><td>ZO-1 PDZ1-2</td><td>—</td><td>—</td><td>—</td></tr><tr><td>JAM</td><td>ZO-1 PDZ2-3</td><td>—</td><td>—</td><td>—</td></tr><tr><td>JAM</td><td>ZO-1 PDZ3-SH3</td><td>—</td><td>—</td><td>—</td></tr></tbody></table>

The strength of the interaction was assessed by analyzing growth at 30°C in the presence of increasing concentrations of 3-AT. (+) indicates growth (—) indicates absence of growth. Cells were left empty when the experiment was not performed. Gy13* is for the T65A mutant. JAM: Junctional adhesion molecule 1.