# Bulletin de veille du réseau d'écotoxicologie terrestre et aquatique N°51

Colette Bertrand, Christian Mougin, Annette Bérard, Céline Pelosi, Soizic

Morin, Olivier Crouzet, Pascale Karmasyn-Veyrines

► To cite this version:

Colette Bertrand, Christian Mougin, Annette Bérard, Céline Pelosi, Soizic Morin, et al.. Bulletin de veille du réseau d'écotoxicologie terrestre et aquatique N°51. 2021, 10.15454/1eeq-fz05. hal-03284368

HAL Id: hal-03284368

https://hal.inrae.fr/hal-03284368v1

Submitted on 12 Jul 2021

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# Bulletin de veille du réseau d'écotoxicologie terrestre et aquatique

<figure><img src="image_2.png" /></figure>

N°51, Juin 2021

Réalisé par l'équipe de veille sur la période du 1er mai au 30 juin 2021.

Colette Bertrand, Christian Mougin (UMR 1402 EcoSys), Annette Berard, Céline Pélosi (UMR 1114 EMMAH), Soizic Morin (UR 1454 EABX), Olivier Crouzet (UPFS - OFB) et Pascale Karmasyn-Veyrines (DipSO)

## Edito

Voici notre 51<sup>ème</sup> bulletin de veille, qui nous espérons toujours informatif !

Nous vous proposons dans ce bulletin une tribune concernant la publication du 1er outil national de sélection des couples espèce/biomarqueur pour le suivi de la qualité des milieux aquatiques : enjeux & perspectives pour la communauté Ecotox. Le texte est également disponible sous forme de fiche thématique en téléchargement sur notre site ECOTOX : https://www6.inrae.fr/ecotox/Productions/Fiches-thematiques/Fiche-thematique-N-33-juin-2021

Nous vous rappelons notre « Peer Community in Ecotoxicology & Environmental Chemistry », un nouveau média ouvert, transparent et gratuit pour la valorisation et la diffusion de vos recherches. Toutes les informations figurent dans la fiche thématique dont la parution est avancée : https://www6.inrae.fr/ecotox/Productions/Fiches-thematiques/Fiche-thematique-N-34-aout-2021 Vous pouvez soumettre vos preprints !

N'oubliez pas de nous transmettre les informations que vous souhaitez diffuser, notamment vos publications que nous pourrions avoir oubliées.

L'équipe vous souhaite une bonne lecture de ce bulletin !

Contact : veille-ecotox@inrae.fr
# SOMMAIRE

## TRIBUNE LIBRE

12

## ERA / PUBLICATIONS SCIENTIFIQUES / COMMUNAUTES MICROBIENNES AQUATIQUES. 18

<table><tr><td>Study of the Tagus River and Entrepenas reservoir ecosystem around the Trillo nuclear power plant using chemometric analysis: Influence on water, sediments, algae and fish</td><td>18</td></tr><tr><td>The link between pharmaceuticals and cyanobacteria: a review regarding ecotoxicological, ecological, and sanitary aspects</td><td>18</td></tr><tr><td>Insights into the microbial degradation and biochemical mechanisms of carbamates</td><td>18</td></tr><tr><td>A trophic transfer study: accumulation of multi-walled carbon nanotubes associated to green algae in water flea Daphnia magna</td><td>18</td></tr><tr><td>Thorium Exposure Drives Fatty Acid and Metal Transfer from Biofilms to the Grazer Lymnaea sp.</td><td>19</td></tr><tr><td>Cytotoxicity and genotoxicity of coated-gold nanoparticles on freshwater algae Pseudokirchneriella subcapitata</td><td>19</td></tr><tr><td>Exploring the role of water chemistry on metal accumulation in biofilms from streams in mining areas</td><td>19</td></tr><tr><td>Toxicity evaluation of nano-TiO2 in the presence of functionalized microplastics at two trophic levels: Algae and crustaceans</td><td>19</td></tr><tr><td>Metagenomic analysis of a mega-city river network reveals microbial compositional heterogeneity among urban and peri-urban river stretch</td><td>20</td></tr><tr><td>Characterizations of heavy metal contamination, microbial community, and resistance genes in a tailing of the largest copper mine in China</td><td>20</td></tr><tr><td>Effects of acid drainage from abandoned coal mines on the microbial community of Shandi River sediment, Shanxi Province</td><td>20</td></tr><tr><td>The impacts of metal-based engineered nanomaterial mixtures on microbial systems: A review</td><td>21</td></tr><tr><td>Effect of pyrethroid on growth and protein content of two unicellular cyanobacteria (blue green algae)</td><td>21</td></tr><tr><td>Dissolved organic matter modulates the impact of herbicides on a freshwater alga: A laboratory study of a three-way interaction</td><td>21</td></tr><tr><td>Environmental concentrations of sulfonamides can alter bacterial structure and induce diatom deformities in freshwater biofilm communities</td><td>22</td></tr><tr><td>The inhibitory effects of the antifouling compound Irgarol 1051 on the marine diatom Skeletonema sp. across a broad range of photosynthetically active radiation</td><td>22</td></tr><tr><td>The microbial community in a green turtle nesting beach in the Mediterranean: application of the Biolog EcoPlate approach for beach pollution</td><td>22</td></tr><tr><td>Combined effects of sulfamethoxazole and erythromycin on a freshwater microalga, Raphidocelis subcapitata: toxicity and oxidative stress</td><td>22</td></tr><tr><td>Ecological risk potentials of petroleum hydrocarbons and heavy metals shape the bacterial communities of marine hydrosphere at Atlantic Ocean, Atlas Cove, Nigeria</td><td>23</td></tr><tr><td>Biodiversity and functional trait effects on copper toxicity in a proof-of-concept multispecies microalgal assay</td><td>23</td></tr><tr><td>Formation of silver nanoparticles in aquatic environments facilitated by algal extracellular polymeric substances: Importance of chloride ions and light</td><td>23</td></tr><tr><td>Evaluation of Multivariate Biomarker Indexes Application in Ecotoxicity Tests with Marine Diatoms Exposed to Emerging Contaminants</td><td>24</td></tr><tr><td>Response of Benthic Diatom Assemblages to Contamination by Metals in a Marine Environment</td><td>24</td></tr><tr><td>Glyphosate-based herbicide exposure affects diatom community development in natural biofilms</td><td>24</td></tr><tr><td>Influence of silver nanoparticle-based coating on calcareous rock surfaces on microbial biofilm colonization in</td><td></td></tr></table>
<table><tr><td>intertidal environments in Campeche, Mexico</td><td>24</td></tr><tr><td>The effect of chromium on photosynthesis and lipid accumulation in two chlorophyte microalgae</td><td>25</td></tr><tr><td>Toxicity of copper on marine diatoms, Chaetoceros calcitrans and Nitzchia closterium from Cochin estuary, India</td><td>25</td></tr><tr><td>Bacteria compete with hematite nanoparticles during their uptake by the ciliate Tetrahymena thermophila</td><td>25</td></tr><tr><td>Pesticide mixture toxicity to algae in agricultural streams? Field observations and laboratory studies with in situ samples and reconstituted water</td><td>26</td></tr></table>

ERA / PUBLICATIONS SCIENTIFIQUES / ECOTOXICOLOGIE SPATIALE 26

<table><tr><td>Detecting landscape scale consequences of insecticide use on invertebrate communities</td><td>26</td></tr><tr><td>Neonicotinoid residues in honey from urban and rural environments</td><td>27</td></tr><tr><td>Occurrence, spatial distribution, Source, and ecological risk assessment of organochlorine pesticides in Dongting Lake, China</td><td>27</td></tr><tr><td>Landscape Composition and Fungicide Exposure Influence Host-Pathogen Dynamics in a Solitary Bee</td><td>27</td></tr><tr><td>Synergism between local- and landscape-level pesticides reduces wild bee floral visitation in pollinator-dependent crops</td><td>27</td></tr></table>

ERA / PUBLICATIONS SCIENTIFIQUES / MICROBIOLOGIE ET CONTAMINANTS 28

<table><tr><td>Metagenomic sequencing reveals detoxifying and tolerant functional genes in predominant bacteria assist Metaphire guillelmi adapt to soil vanadium exposure</td><td>28</td></tr><tr><td>Effects of increasing concentrations of fungicide Quadris(R) on bacterial functional profiling in loamy sand soil</td><td>28</td></tr><tr><td>Determination of Bactericidal Effects of Some Pesticides on Useful and Pathogenic Bacteria</td><td>28</td></tr><tr><td>Field-scale studies on the change of soil microbial community structure and functions after stabilization at a chromium-contaminated site</td><td>28</td></tr><tr><td>Effects of sulfonylurea herbicides chlorsulfuron and sulfosulfuron on enzymatic activities and microbial communities in two agricultural soils</td><td>29</td></tr><tr><td>Responses of soil microbial community to combination pollution of galaxolide and cadmium</td><td>29</td></tr><tr><td>Measurement and modeling of hormesis in soil bacteria and fungi under single and combined treatments of Cd and Pb</td><td>29</td></tr><tr><td>Characterizations of heavy metal contamination, microbial community, and resistance genes in a tailing of the largest copper mine in China</td><td>29</td></tr><tr><td>Field study on the soil bacterial associations to combined contamination with heavy metals and organic contaminants</td><td>30</td></tr><tr><td>Effects of long-term discharge of acid mine drainage from abandoned coal mines on soil microorganisms: microbial community structure, interaction patterns, and metabolic functions</td><td>30</td></tr><tr><td>Soil characteristics and microbial community response in rare earth mining areas in southern Jiangxi Province, China</td><td>30</td></tr><tr><td>Enzyme activities and microbial functional diversity in metal(loid) contaminated soils near to a copper smelter</td><td>30</td></tr><tr><td>Microbial Ecotoxicity of Biochars in Agricultural Soil and Interactions with Linear Alkylbenzene Sulfonates</td><td>31</td></tr><tr><td>Side effects of traditional pesticides on soil microbial respiration in orchards on the Russian Black Sea coast</td><td>31</td></tr><tr><td>Gut microbial community response to herbicide exposure in a ground beetle</td><td>31</td></tr><tr><td>Field study on the soil bacterial associations to combined contamination with heavy metals and organic contaminants</td><td>31</td></tr><tr><td>CuO, ZnO, and gamma-Fe2O3 nanoparticles modified the underground biomass and rhizosphere microbial community of Salvia miltiorrhiza (Bge.) after 165-day exposure</td><td>32</td></tr><tr><td>Microbial communities of the drinking water with gradient radon concentration are primarily contributed by radon</td><td></td></tr></table>
<table><tr><td>and heavy metal content</td><td>32</td></tr><tr><td>Salt stress-induced changes in microbial community structures and metabolic processes result in increased soil cadmium availability</td><td>32</td></tr><tr><td>Soil characteristics and microbial community response in rare earth mining areas in southern Jiangxi Province, China</td><td>32</td></tr><tr><td>Microbial ecotoxicity of biochars in agricultural soil and interactions with linear alkylbenzene sulfonates</td><td>33</td></tr><tr><td>Effects of heavy metals on bacterial community structure in the rhizosphere of Salsola collina and bulk soil in the Jinchuan mining area</td><td>33</td></tr><tr><td>Study on the regulatory mechanism of the earthworm microbial community in vitro and in vivo under cadmium stress</td><td>33</td></tr><tr><td>Bacterial response to soil property changes caused by wood ash from wildfire in forest soils around mining areas: Relevance of bacterial community composition, carbon and nitrogen cycling</td><td>33</td></tr><tr><td>Soil bacterial community functions and distribution after mining disturbance</td><td>34</td></tr><tr><td>Compositional and functional responses of bacterial community to titanium dioxide nanoparticles varied with soil heterogeneity and exposure duration</td><td>34</td></tr><tr><td>Spatial variation in microbial community in response to As and Pb contamination in paddy soils near a pb-zn mining site</td><td>34</td></tr><tr><td>Diversity patterns and drivers of soil microbial communities in urban and suburban park soils of Shanghai, China</td><td>34</td></tr><tr><td>High-throughput sequencing clarifies the spatial structures of microbial communities in cadmium-polluted rice soils</td><td>34</td></tr><tr><td>Study of pollutant accumulation characteristics and microbial community impact at three bioretention facilities</td><td>35</td></tr><tr><td>Microbiological indicators of heavy metals and carbon-containing preparations applied to agrosoddy-podzolic soils differing in humus content</td><td>35</td></tr><tr><td>Land use in urban areas impacts the composition of soil bacterial communities involved in nitrogen cycling. A case study from Lefkosia (Nicosia) Cyprus</td><td>35</td></tr><tr><td>Spatial distribution characteristics of the microbial community and multi-phase distribution of toxic metals in the geochemical gradients caused by acid mine drainage, South China</td><td>36</td></tr><tr><td>Insights on the effects of ZnO nanoparticle exposure on soil heterotrophic respiration as revealed by soil microbial communities and activities</td><td>36</td></tr><tr><td>The effects of vehicular emissions on the activity and diversity of the roadside soil microbial community</td><td>36</td></tr><tr><td>Synergistic effects of antimony and arsenic contaminations on bacterial, archaeal and fungal communities in the rhizosphere of Miscanthus sinensis: Insights for nitrification and carbon mineralization</td><td>36</td></tr><tr><td>Impact of pesticide/fertilizer mixtures on the rhizosphere microbial community of field-grown sugarcane</td><td>37</td></tr><tr><td>Uranium(VI) bioassociation by different fungi - a comparative study into molecular processes</td><td>37</td></tr><tr><td>Ecotoxicity of as-synthesised copper nanoparticles on soil bacteria</td><td>37</td></tr><tr><td>Metagenomic insights into taxonomic diversity and metabolic potential of bacterial communities associated with tannery waste-contaminated soils</td><td>37</td></tr><tr><td>Impacts of Redox Conditions on Arsenic and Antimony Transformation in Paddy Soil: Kinetics and Functional Bacteria</td><td>38</td></tr><tr><td>Changes in soil and rat gut microbial diversity after long-term exposure to the chiral fungicide epoxiconazole</td><td>38</td></tr><tr><td>Microbial Features Indicating the Recovery of Soil Ecosystem Strongly Affected by Mining and Ore Processing</td><td>38</td></tr></table>

ERA / PUBLICATIONS SCIENTIFIQUES / MICROBIOLOGIE ET CONTAMINANTS / ANTIBIOTIQUES ET ANTIBIORESISTANCES

Distribution of the microbial community and antibiotic resistance genes in farmland surrounding gold tailings: A metagenomics approach
<table><tr><td>Chicken Manure and Mushroom Residues Affect Soil Bacterial Community Structure but Not the Bacterial Resistome When Applied at the Same Rate of Nitrogen for 3 Years</td><td>39</td></tr><tr><td>Habitat determines the relationships among bacteria, resistance genes and mobile genetic elements in the soil-plant system</td><td>39</td></tr><tr><td>Bacterial community assembly and antibiotic resistance genes in the lettuce-soil system upon antibiotic exposure</td><td>39</td></tr><tr><td>Distinct resistomes and microbial communities of soils, wastewater treatment plants and households suggest development of antibiotic resistances due to distinct environmental conditions in each environment</td><td>39</td></tr><tr><td>Polystyrene microplastics alleviate the effects of sulfamethazine on soil microbial communities at different CO2 concentrations</td><td>40</td></tr><tr><td>Soil Enzymatic Activities and Microbial Community Structure in Soils Polluted with Tetracycline Antibiotics</td><td>40</td></tr><tr><td>Effects of Sulfamethoxazole on Growth and Antibiotic Resistance of A Natural Microbial Community</td><td>40</td></tr><tr><td>Tetracycline-resistant bacteria and ribosomal protection protein genes in soils from selected agricultural fields and livestock farms</td><td>40</td></tr><tr><td>Manure-Based Amendments Influence Surface-Associated Bacteria and Markers of Antibiotic Resistance on Radishes Grown in Soils with Different Textures</td><td>41</td></tr><tr><td>Effects of sulfamethoxazole on growth and antibiotic resistance of a natural microbial community</td><td>41</td></tr><tr><td>Application of manure from cattle administered antibiotics has sustained multi-year impacts on soil resistome and microbial community structure</td><td>41</td></tr><tr><td>Gain and loss of antibiotic resistant genes in multidrug resistant bacteria: One Health perspective</td><td>41</td></tr><tr><td>Antibiotrophy: key function for antibiotic-resistant bacteria to colonize soils-case of sulfamethazine-degrading microbacterium sp. C448</td><td>42</td></tr><tr><td>Can heavy metal pollution induce bacterial resistance to heavy metals and antibiotics in soils from an ancient land-mine?</td><td>42</td></tr><tr><td>Potential environmental and human health risks caused by antibiotic-resistant bacteria (arb), antibiotic resistance genes (args) and emerging contaminants (ecs) from municipal solid waste (msw) landfill</td><td>42</td></tr></table>

## ERA / PUBLICATIONS SCIENTIFIQUES / MICROBIOLOGIE ET CONTAMINANTS / BIOCONTROLE 43

<table><tr><td>Role of two plant growth-promoting bacteria in remediating cadmium-contaminated soil combined with <i>miscanthus floridulus</i> (lab.)</td><td>43</td></tr><tr><td>Physiological and metagenomic strategies uncover the rhizosphere bacterial microbiome succession underlying three common environmental stresses in cassava</td><td>43</td></tr><tr><td>Plant growth-promoting rhizobacteria effect on maize growth and microbial biomass in a chromium-contaminated soil</td><td>43</td></tr><tr><td>A pesticide biopurification system: a source of biosurfactant-producing bacteria with environmental biotechnology applications</td><td>43</td></tr></table>

## ERA / PUBLICATIONS SCIENTIFIQUES / MICROBIOLOGIE ET CONTAMINANTS / BIOREMEDIATION 44

<table><tr><td>Recruiting endophytic bacteria of wetland plants to phytoremediate organic pollutants</td><td>44</td></tr><tr><td>Degradation of chlorpyriphos and polyethylene by endosymbiotic bacteria from citrus mealybug</td><td>44</td></tr><tr><td>The microbial community from the early-plant colonizer (Baccharis linearis) is required for plant establishment on copper mine tailings</td><td>44</td></tr><tr><td>Effects of arbuscular mycorrhizal fungi on maize (<i>zea mays</i> I.) Under zinc deficient and toxic field conditions</td><td>45</td></tr><tr><td>Fungal biosorption of cadmium(II) onto <i>Fennelia nivea</i> from aqueous solution: equilibrium, thermodynamics, and</td><td></td></tr></table>
<table><tr><td>kinetics</td><td>45</td></tr><tr><td>Strategies in microbial degradation enhancement of chlorpyrifos - a review based on the primary approaches in soil bioremediation</td><td>45</td></tr><tr><td>Motor oil wastewater treatment in a packed bed bioreactor using immobilized native microbial consortium</td><td>45</td></tr><tr><td>Insight into Cr(VI) biosorption onto algal-bacterial granular sludge: Cr(VI) bioreduction and its intracellular accumulation in addition to the effects of environmental factors</td><td>46</td></tr><tr><td>Improved immobilization of soil cadmium by regulating soil characteristics and microbial community through reductive soil disinfestation</td><td>46</td></tr><tr><td>Effects of magnetic biochar-microbe composite on Cd remediation and microbial responses in paddy soil</td><td>46</td></tr><tr><td>Biodegradation pathway of polycyclic aromatic hydrocarbons by ligninolytic fungus podoscypha elegans strain ftg4 and phytotoxicity evaluation of their metabolites</td><td>46</td></tr><tr><td>Microbial and abiotic factors of flooded soil that affect redox biodegradation of lindane</td><td>47</td></tr><tr><td>Phytostabilization of acidic mine tailings with biochar, biosolids, lime, and locally-Sourced microbial inoculum: Do amendment mixtures influence plant growth, tailing chemistry, and microbial composition?</td><td>47</td></tr><tr><td>Current insights into the microbial degradation for butachlor: strains, metabolic pathways, and molecular mechanisms</td><td>47</td></tr><tr><td>The multi metal-resistant bacterium Cupriavidus metallidurans CH34 affects growth and metal mobilization in Arabidopsis thaliana plants exposed to copper</td><td>47</td></tr><tr><td>Role of plant growth promoting bacteria in driving speciation gradients across soil-rhizosphere-plant interfaces in zinc-contaminated soils</td><td>48</td></tr><tr><td>Bacterial isolates from Argentine Pampas and their ability to degrade glyphosate</td><td>48</td></tr><tr><td>Carboxylic acid reduction and sulfate-reducing bacteria stabilization combined remediation of Cr (VI)-contaminated soil</td><td>48</td></tr><tr><td>Biodegradation Pathway of Polycyclic Aromatic Hydrocarbons by Ligninolytic Fungus Podoscypha elegans Strain FTG4 and Phytotoxicity Evaluation of their Metabolites</td><td>49</td></tr><tr><td>Contribution of Nano-Zero-Valent Iron and Arbuscular Mycorrhizal Fungi to Phytoremediation of Heavy Metal-Contaminated Soil</td><td>49</td></tr><tr><td>Microbial and abiotic factors of flooded soil that affect redox biodegradation of lindane</td><td>49</td></tr><tr><td>Fungal biostimulant-driven phytoextraction of heavy metals from tannery solid waste contaminated soils</td><td>49</td></tr><tr><td>An overview of neonicotinoids: biotransformation and biodegradation by microbiological processes</td><td>50</td></tr><tr><td>Synergistic Effects of Zinc Oxide Nanoparticles and Bacteria Reduce Heavy Metals Toxicity in Rice (Oryza sativa L.) Plant</td><td>50</td></tr><tr><td>Effects of Soil Properties on Phytoextraction of Cd and the Associated Soil Bacterial Communities across Four Soil Types</td><td>50</td></tr><tr><td>Ureolytic bacteria from electronic waste area, their biological robustness against potentially toxic elements and underlying mechanisms</td><td>50</td></tr><tr><td>Metagenomics reveals functional profiling of microbial communities in OCP contaminated sites with rapeseed oil and tartaric acid biostimulation</td><td>51</td></tr><tr><td>Metagenomic analysis of microbial community and its role in bioelectrokinetic remediation of tannery contaminated soil</td><td>51</td></tr><tr><td>Effects of different dissolved organic matter on microbial communities and arsenic mobilization in aquifers</td><td>51</td></tr><tr><td>Heavy Metal-Resistant Filamentous Fungi as Potential Mercury Bioremediators</td><td>51</td></tr><tr><td>Microbial bioremediation of heavy metals</td><td>52</td></tr><tr><td>The participation of cyanobacteria in reducing the concentration of fusariotoxins and heavy metal ions in aqueous solutions (model experiments)</td><td>52</td></tr><tr><td>Heavy metals in soils and the remediation potential of bacteria associated with the plant microbiome</td><td>52</td></tr><tr><td>Plant growth-promoting rhizobacteria effect on maize growth and microbial biomass in a chromium-contaminated</td><td></td></tr></table>
<table><tr><td>soil</td><td>52</td></tr><tr><td>A review on mechanism of biomineralization using microbial-induced precipitation for immobilizing lead ions</td><td>53</td></tr><tr><td>Microbial community responses to land-use types and its ecological roles in mining area</td><td>53</td></tr><tr><td>The physiological response of Ectomycorrhizal fungus Lepista sordida to Cd and Cu stress</td><td>53</td></tr><tr><td>Microbial degradation of recalcitrant pesticides: a review</td><td>53</td></tr><tr><td>Effect of poultry manure compost and arbuscular mycorrhizal fungi on Cu immobilization and soil microbial communities in a Cu-contaminated soil using the metallophyte Oenothera picensis</td><td>54</td></tr><tr><td>Multiple heavy metals immobilization based on microbially induced carbonate precipitation by ureolytic bacteria and the precipitation patterns exploration</td><td>54</td></tr><tr><td>Removal of Iron from Aqueous Solution by Novel Bacteria Isolated from Marine Macro Alga</td><td>54</td></tr><tr><td>Bio-mercury remediation suitability index: a novel proposal that compiles the pgpr features of bacterial strains and its potential use in phytoremediation</td><td>54</td></tr><tr><td>Effects of microbial spatial distribution on organic biodegradation and immobilization of trace metals in co-contaminated soils</td><td>55</td></tr><tr><td>Characterization of soil bacteria with potential to degrade benzoate and antagonistic to fungal and bacterial phytopathogens</td><td>55</td></tr><tr><td>Review on Pesticides Application in Agriculture and its Effect on Environment with Brief on their Microbial Degradation</td><td>55</td></tr><tr><td>Frenemies: interactions between rhizospheric bacteria and fungi from metalliferous soils</td><td>55</td></tr><tr><td>Green remediation of toxic metals contaminated mining soil using bacterial consortium and Brassica juncea</td><td>56</td></tr><tr><td>The effects of vermicompost and shell powder addition on Cd bioavailability, enzyme activity and bacterial community in Cd-contaminated soil: A field study</td><td>56</td></tr><tr><td>Improvement of alfalfa resistance against Cd stress through rhizobia and arbuscular mycorrhiza fungi co-inoculation in Cd-contaminated soil</td><td>56</td></tr><tr><td>Biodegradation of 4-chloro-2-methylphenoxyacetic acid by endophytic fungus Phomopsis sp. In liquid medium and soil</td><td>56</td></tr><tr><td>Insight into metal immobilization and microbial community structure in soil from a steel disposal dump phytostabilized with composted, pyrolyzed or gasified wastes</td><td>57</td></tr></table>

ERA / PUBLICATIONS SCIENTIFIQUES / PESTICIDES/CLIMAT ET VDT/ENCHY 57

<table><tr><td>Measurement of multixenobiotic resistance activity in enchytraeids as a tool in soil ecotoxicology</td><td>57</td></tr><tr><td>Ecotoxicological effects of the pyrethroid insecticide tefluthrin to the earthworm Eisenia fetida: a chiral view</td><td>57</td></tr><tr><td>Biomarker responses and metabolism in Lumbricus terrestris exposed to drugs of environmental concern, an in vivo and in vitro approach</td><td>58</td></tr><tr><td>Pesticides and soil invertebrates: a hazard assessment</td><td>58</td></tr><tr><td>Soil biodiversity: state-of-the-art and possible implementation in chemical risk assessment</td><td>58</td></tr><tr><td>Effects of glyphosate-based herbicides and their active ingredients on earthworms, water infiltration and glyphosate leaching are influenced by soil properties</td><td>58</td></tr><tr><td>The pursuit of Alachlor herbicide toxicity on Eisenia fetida and its biochemical responses</td><td>59</td></tr></table>

ERA / PUBLICATIONS SCIENTIFIQUES / PLASTIQUES 59

<table><tr><td>Attached and planktonic bacterial communities on bio-based plastic granules and micro-debris in seawater and freshwater</td><td>59</td></tr><tr><td>Comparison of the effects of continuous and accumulative exposure to nanoplastics on microalga Chlorella pyrenoidosa during chronic toxicity</td><td>59</td></tr></table>
<table><tbody><tr><td>Effect of microplastics on growth and biochemical composition of microalga <i>Acutodesmus obliquus</i></td><td>59</td></tr><tr><td>Plastic degradation by extremophilic bacteria</td><td>60</td></tr><tr><td>Effects of Plastic Debris on the Biofilm Bacterial Communities in Lake Water</td><td>60</td></tr><tr><td>Mechanism of the inhibition and detoxification effects of the interaction between nanoplastics and microalgae <i>Chlorella pyrenoidosa</i></td><td>60</td></tr><tr><td>Microplastics contamination in the protected agricultural soils and its effects on bacterial community diversity</td><td>60</td></tr><tr><td>Soil type driven change in microbial community affects poly(butylene adipate-co-terephthalate) degradation potential</td><td>61</td></tr><tr><td>Aged microplastics decrease the bioavailability of coexisting heavy metals to microalga <i>Chlorella vulgaris</i></td><td>61</td></tr><tr><td>Effect thresholds for the earthworm <i>Eisenia fetida</i>: Toxicity comparison between conventional and biodegradable microplastics</td><td>61</td></tr><tr><td>The rise of artificial soil carbon inputs: Reviewing microplastic pollution effects in the soil environment</td><td>61</td></tr><tr><td>Microplastics contamination in the protected agricultural soils and its effects on bacterial community diversity</td><td>62</td></tr><tr><td>Soil microbial community and network changes after long-term use of plastic mulch and nitrogen fertilization on semiarid farmland</td><td>62</td></tr><tr><td>Effects of biofilm colonization on the sinking of microplastics in three freshwater environments</td><td>62</td></tr><tr><td>Bacterial and fungal assemblages and functions associated with biofilms differ between diverse types of plastic debris in a freshwater system</td><td>63</td></tr><tr><td>Genomic and proteomic profiles of biofilms on microplastics are decoupled from artificial surface properties</td><td>63</td></tr><tr><td>Combined effects of microplastics and biochar on the removal of polycyclic aromatic hydrocarbons and phthalate esters and its potential microbial ecological mechanism</td><td>63</td></tr><tr><td>Effect of plastic film mulching on the distribution of plastic residues in agricultural fields</td><td>64</td></tr><tr><td>The microplastisphere: Biodegradable microplastics addition alters soil microbial community structure and function</td><td>64</td></tr><tr><td>Trading tree growth for soil degradation: Effects at 10 years of black plastic mulch on fine roots, earthworms, organic matter and nitrate in a multi-species riparian buffer</td><td>64</td></tr><tr><td>Diversity and structure of microbial biofilms on microplastics in riverine waters of the Pearl River Delta, China</td><td>65</td></tr></tbody></table>

## DROIT ET POLITIQUE DE L'ENVIRONNEMENT .................................................................................. 65

<table><tbody><tr><td>Protection des abeilles et autres insectes pollinisateurs et préservation des services de pollinisation lors de l'utilisation des produits phytopharmaceutiques - Consultations publiques</td><td>65</td></tr><tr><td>Identification des alternatives au glyphosate pour les usages non agricoles</td><td>65</td></tr><tr><td>EPA releases updated occupational pesticide handler and post-application exposure calculators</td><td>66</td></tr><tr><td>Substance active terbuthylazine : conditions d'approbation</td><td>66</td></tr><tr><td>Approbation renouvelée de la substance active cyazofamid</td><td>66</td></tr><tr><td>Approbation renouvelée de la substance active Streptomyces souche K61</td><td>66</td></tr><tr><td>La commission européenne présente son plan d'actions "Zero pollution" à l'horizon 2050</td><td>67</td></tr></tbody></table>

## REGLEMENTATION / DROIT ................................................................................................................ 67

<table><tbody><tr><td>Produits phytopharmaceutiques : AMM d'une durée maximale de 120 jours délivrées par le ministère dans des situations d'urgence phytosanitaire</td><td>67</td></tr><tr><td>Alternatives aux phytosanitaires : un arrêté facilite le recours aux substances naturelles</td><td>67</td></tr><tr><td>Périodes d'approbation des substances actives «Adoxophyes orana granulovirus» et «flutriafol»</td><td>67</td></tr></tbody></table>
AVIS / EXPERTISES / NORMES.................................................................................................................. 68

Peer review of the pesticide risk assessment of the active substance carbon dioxide ..................................................68

Des solutions alternatives aux néonicotinoïdes pour lutter contre la jaunisse dans les cultures de betteraves ............68

A systems-based approach to the environmental risk assessment of multiple stressors in honey bees......................68

Pesticide risk assessment of the active substance *Bacillus amyloliquefaciens* strain IT-45 : peer review ......................68

Incendie Lubrizol et NL Logistique : bilan des mesures de polluants et d'odeurs dans l'air ambiant et retombées atmosphériques................................................................................................................................................69

Review of the existing MRL for valifenalate according to Article 12 of Regulation (EC) No 396/2005 ......................69

PUBLICATIONS DU RESEAU ECOTOX .................................................................................................................. 69

Impact of repeated irrigation of lettuce cultures with municipal wastewater on soil bacterial community diversity and composition................................................................................................................................................69

Assessment of pesticides volatilization potential based on their molecular properties using the TyPol tool ..............69

Evaluation of trace metal accumulation in six vegetable crops intercropped with phytostabilizing plant species, in a French urban wasteland ................................................................................................................................................70

Molecular bases of P450-mediated resistance to the neonicotinoid insecticide imidacloprid in the mosquito Ae. Aegypti................................................................................................................................................70

Global data on earthworm abundance, biomass, diversity and corresponding environmental properties ..............71

Quantifying pesticide emission fractions for tropical conditions ................................................................................71

The Isotopic Signature of Lead Emanations during the Fire at Notre Dame Cathedral in Paris, France......................71

New perspectives on the calculation of bioaccumulation metrics for active substances in living organisms..............71

Impact of cadmium on forage kale (*Brassica oleracea* var. viridis cv "Prover") after 3-, 10- and 56-day exposure to a Cd-spiked field soil................................................................................................................................................72

Origin, fate and ecotoxicity of manganese from legacy metallurgical wastes ................................................................72

Phase Transfer and Biodegradation of Pesticides in Water-Sediment Systems Explored by Compound-Specific Isotope Analysis and Conceptual Modeling ................................................................................................................72

Simple models efficiently predict free cadmium Cd2+ in the solutions of low-contaminated agricultural soils ........73

New challenges of marine ecotoxicology in a global change context ................................................................................73

Individual quality and phenology mediate the effect of radioactive contamination on body temperature in Chernobyl barn swallows................................................................................................................................................74

Individual performances and biochemical pathways as altered by field-realistic exposures of current-use fungicides and their mixtures in a non-target species, Gammarus fossarum ................................................................................................................74

Pesticide risk assessment in honeybees: Toward the use of behavioral and reproductive performances as assessment endpoints ................................................................................................................................................74

Molecular impacts of dietary exposure to nanoplastics combined with arsenic in Canadian oysters (*Crassostrea virginica*) and bioaccumulation comparison with Caribbean oysters (*Isognomon alatus*)................................................75

Molecular Impacts of Dietary Exposure to Nanoplastics Combined or Not with Arsenic in the Caribbean Mangrove Oysters (*Isognomon alatus*)................................................................................................................................................75

Characterization of chlordecone distribution and elimination in ewes during daily exposure and depuration ........75

Response from the Authors of the article "Critical review of the role of personal protective Equipment (PPE) in the prevention of risks related to agricultural pesticide use" to the letter to the editor from the European crop protection association (ECPA) Occupational and bystander exposure expert group (OBEEG) ........................................76

Ecotoxicological risk assessment of contaminants of emerging concern identified by "suspect screening" from urban
<table><tr><td>wastewater treatment plant effluents at a territorial scale</td><td>76</td></tr><tr><td>Evaluation of the OECD QSAR toolbox automatic workflow for the prediction of the acute toxicity of organic chemicals to fathead minnow</td><td>77</td></tr><tr><td>Dissolved organic matter modulates the impact of herbicides on a freshwater alga: A laboratory study of a three-way interaction</td><td>77</td></tr><tr><td>Adsorption of a triazole antifungal agent, difenoconazole, on soils from a cereal farm: Protective effect of hemp felt</td><td>77</td></tr><tr><td>Using textile industrial sludge, sewage wastewater, and sewage sludge as inoculum to degrade recalcitrant textile dyes in a co-composting process an assessment of biodegradation efficiency and compost phytotoxicity</td><td>78</td></tr><tr><td>From environmental bioavailability of metal(loid)s to their ecogenotoxicological effects in land snails</td><td>78</td></tr><tr><td>Biodiversity monitoring using environmental DNA</td><td>78</td></tr><tr><td>Greening effect of slag cement-based concrete: Environmental and ecotoxicological impact</td><td>79</td></tr><tr><td>Impact of ancient iron smelting wastes on current soils: Legacy contamination, environmental availability and fractionation of metals</td><td>79</td></tr><tr><td>Uptake and effects of graphene oxide nanomaterials alone and in combination with polycyclic aromatic hydrocarbons in zebrafish</td><td>79</td></tr><tr><td>Phytoavailability of cd, cr, cu, hg, mn, mo, ni, pb, tl and zn in arable crop systems amended for 13 to 15 years with organic waste products</td><td>80</td></tr><tr><td>The role of ponds in pesticide dissipation at the agricultural catchment scale: a critical review</td><td>80</td></tr><tr><td>Record of trace organic contaminants in a river sediment core: From historical wastewater management to historical use</td><td>80</td></tr><tr><td>Modeling acetylcholine esterase inhibition resulting from exposure to a mixture of atrazine and chlorpyrifos using a physiologically-based kinetic model in fish</td><td>81</td></tr><tr><td>Thermal plasticity and sensitivity to insecticides in populations of an invasive beetle: Cyfluthrin increases vulnerability to extreme temperature</td><td>81</td></tr><tr><td>The added value of Bayesian inference for estimating biotransformation rates of organic contaminants in aquatic invertebrates</td><td>81</td></tr><tr><td>Occurrence and removal of emerging pollutants in urban sewage treatment plants using LC-QToF-MS suspect screening and quantification</td><td>82</td></tr><tr><td>Pollutant Dissipation at the Sediment-Water Interface: A Robust Discrete Continuum Numerical Model and Recirculating Laboratory Experiments</td><td>82</td></tr><tr><td>A pilot experiment to assess the efficiency of pharmaceutical plant wastewater treatment and the decreasing effluent toxicity to periphytic biofilms</td><td>83</td></tr><tr><td>Antibiotrophy: Key Function for Antibiotic-Resistant Bacteria to Colonize Soils-Case of Sulfamethazine-Degrading Microbacterium sp. C448</td><td>83</td></tr><tr><td>The utility of Dreissena polymorpha for assessing the viral contamination of rivers by measuring the accumulation of f-specific rna bacteriophages</td><td>83</td></tr><tr><td>Fluorescence excitation/emission matrices as a tool to monitor the removal of organic micropollutants from wastewater effluents by adsorption onto activated carbon</td><td>84</td></tr><tr><td>Does copper contamination affect soil CO2 emissions ? A literature review</td><td>84</td></tr><tr><td>Graphene-based nanomaterials modulate internal biofilm interactions and microbial diversity</td><td>85</td></tr><tr><td>Effects of silver nanoparticles on performance of anaerobic digestion of sewage sludge and associated microbial communities</td><td>85</td></tr></table>

<table><tr><td>OUVRAGES / RAPPORTS / ACTES DE CONGRES</td><td>85</td></tr><tr><td>Pesticides in the Natural Environment</td><td>86</td></tr></table>
<table><tr><td>Reproductive and Developmental Toxicology</td><td>86</td></tr><tr><td>Exploring the benefits of biocontrol for sustainable agriculture</td><td>86</td></tr><tr><td>Biopesticides in Organic Farming: Recent Advances</td><td>86</td></tr></table>

REVUE DE PRESSE / ALTERNATIVES / BIOPESTICIDES 87

<table><tr><td>Nicotinamide can 'immunize' plants to protect from fungal disease</td><td>87</td></tr><tr><td>Pollen-sized technology protects bees from deadly insecticides</td><td>87</td></tr><tr><td>Study points to potential treatment for citrus disease</td><td>87</td></tr></table>

REVUE DE PRESSE / ASSOCIATIONS 87

<table><tr><td>Victoire du CRIIGEN : Interdiction du Roundup Pro 360 !</td><td>88</td></tr><tr><td>Vineyard Pesticides Linked to Parkinson's</td><td>88</td></tr><tr><td>Pesticide contamination in waterways raises new alarm for aquatic life, citing poor regulation</td><td>88</td></tr><tr><td>Produits phytos et santé : le lien se précise pour six maladies graves</td><td>88</td></tr><tr><td>Prosulfocarbe : l'herbicide volatile qui contamine les productions bios</td><td>89</td></tr><tr><td>Past use of lead arsenate pesticides continue to contaminate residential areas 70 years later</td><td>89</td></tr><tr><td>Ominous first step in EU renewal process of glyphosate: 4 member states suggest no risk for human health</td><td>89</td></tr><tr><td>Propazine Cancelled by EPA - Advocates Urge Agency to Finish the Job by Banning Atrazine and SimazineBeyond Pesticides, June 15, 2021</td><td>89</td></tr><tr><td>Gil Rivière-Wekstein : le débat sur le glyphosate a été détourné</td><td>90</td></tr><tr><td>Les Suisses disent non à une agriculture sans pesticides à court terme</td><td>90</td></tr><tr><td>A study analyzes the risk of karst groundwater contamination to human consumption</td><td>90</td></tr><tr><td>Plan pollinisateur : encore un raté du gouvernement sur le sujet des pesticides et des abeilles</td><td>90</td></tr><tr><td>Scandale : des pesticides autorisés par l'UE en dépit de leur dangerosité et d'évaluations sujettes à caution !</td><td>91</td></tr><tr><td>Court Blocks Trump-era, Toxic Citrus Pesticide, Defended by Biden EPA</td><td>91</td></tr><tr><td>Biodiversité Le Parlement européen demande des objectifs contraignants pour 2030</td><td>91</td></tr><tr><td>Un mouvement visionnaire pour une alimentation durable : comment transformer nos systèmes alimentaires d'ici 2045</td><td>91</td></tr><tr><td>Exposure to certain pesticides increase the risk of thyroid cancer</td><td>92</td></tr><tr><td>Coffee Leaf Rust Hits Hawaii - EPA Approves Emergency Fungicide While Hyperparasite Biocontrol Shows Promise</td><td>92</td></tr><tr><td>More evidence documents glyphosate's link to adverse birth outcomes</td><td>92</td></tr><tr><td>New commercial pesticide toxicity analysis highlights need to shift to organic products</td><td>92</td></tr><tr><td>Manche : jusqu'à 100 000 euros requis contre l'usage de produits phytos interdits</td><td>93</td></tr><tr><td>Le crédit d'impôt glyphosate de 2 500 € est opérationnel</td><td>93</td></tr><tr><td>Conventional Meats Contaminated with Multi-Drug Resistant Bacteria, at Significantly Higher Rates than Organic Meats</td><td>93</td></tr><tr><td>Ecological mystery unravels, with toxic pesticide use at the center</td><td>93</td></tr><tr><td>Hormone disrupting chemicals may also harm children's brains - scientists call for action</td><td>93</td></tr><tr><td>EU zero pollution action plan lacks action, NGOs say</td><td>94</td></tr><tr><td>EPA to review Roundup weedkiller to assess potential 'ecological risks'</td><td>94</td></tr><tr><td>Pourquoi les scientifiques cherchent-ils à s'affranchir du terme « métaux lourds » ?</td><td>94</td></tr><tr><td>Pesticides are becoming increasingly toxic for the world's most important insects</td><td>95</td></tr><tr><td>Glyphosate doesn't just kill plants. It harms beetles, too</td><td>95</td></tr><tr><td>Néonicotinoïdes : le Conseil d'Etat confirme leur utilisation temporaire pour les betteraves sucrières</td><td>95</td></tr></table>
<table><tr><td>29 ONG interpellent l'Anses sur des pesticides en vente libre</td><td>95</td></tr><tr><td>Générations Futures réagit à la publication ce jour du PNSE 4</td><td>96</td></tr><tr><td>Victoire juridique dans le dossier des néonicotinoïdes</td><td>96</td></tr><tr><td>L'UFC-Que Choisir opte pour la schizophrénie</td><td>96</td></tr><tr><td>Breakdown products (metabolites) from pesticides may be more toxic than parent compound, study finds</td><td>96</td></tr><tr><td>Federal Court Gives EPA 60-Day Deadline to Decide the Fate of Chlorpyrifos</td><td>97</td></tr><tr><td>Research shows adverse impacts of glyphosate on the human gut microbiome</td><td>97</td></tr><tr><td>Plastics and toxic chemicals are killing fish — and poisoning us</td><td>97</td></tr></table>

REVUE DE PRESSE / RECHERCHE ET MEDIAS 97

<table><tr><td>Deuxième stratégie nationale sur les perturbateurs endocriniens : quel bilan à mi-parcours ?</td><td>97</td></tr><tr><td>Antibiotic-resistant bacteria found in cattle</td><td>98</td></tr><tr><td>Arrêté abeilles : le texte « préservant les pollinisateurs et les conditions de travail » des agriculteurs soumis à consultation publique</td><td>98</td></tr><tr><td>Publication de l'expertise collective Inserm « Pesticides et effets sur la santé : nouvelles données »</td><td>98</td></tr><tr><td>Les 27 ouvrent la voie à de nouveaux efforts de protection</td><td>98</td></tr><tr><td>Étude : L'agroécologie pas incompatible avec lasouveraineté alimentaire</td><td>99</td></tr><tr><td>Seabird eggs contaminated with cocktail of plastic additives</td><td>99</td></tr><tr><td>Public concern on human health impact of plastic pollution</td><td>99</td></tr><tr><td>Several persistent chemicals were found in fetal organs</td><td>99</td></tr><tr><td>Pollinisateurs : l'arrêté abeilles nouvelle version inquiète les apiculteurs</td><td>100</td></tr><tr><td>Le glyphosate n'est pas jugé cancérogène par les régulateurs</td><td>100</td></tr><tr><td>L'État lance une consultation publique sur la réglementation relative aux pulvérisateurs de pesticides</td><td>100</td></tr><tr><td>Les apiculteurs et les arboriculteurs dénoncent la révision de l'arrêté « abeilles »</td><td>100</td></tr><tr><td>Research establishes safe water thresholds for antimicrobials, to help mitigate resistance</td><td>101</td></tr><tr><td>MISP : un outil de calcul d'impact sur les eaux souterraines</td><td>101</td></tr><tr><td>Research: Journey of PFAS in wastewater facilities highlights regulation challenges</td><td>101</td></tr><tr><td>Effects of nanoplastics on Canadian and Guadeloupean oysters</td><td>102</td></tr><tr><td>Produits phytosanitaires Projet de stockage près de Rouen : l'interdiction confirmée en appel</td><td>102</td></tr><tr><td>Honeybees are accumulating airborne microplastics on their bodies</td><td>102</td></tr><tr><td>Breast cancer risk in association with atmospheric pollution exposure: a meta-analysis of effect estimates followed by a health impact assessment</td><td>102</td></tr><tr><td>Pollution aux pesticides dans le port de Rouen : la caractérisation des impacts environnementaux à affiner</td><td>103</td></tr><tr><td>Les arbitrages de la France pour la future PAC suscitent de vives crispations</td><td>103</td></tr><tr><td>Study reveals high levels of contaminants in killer whales</td><td>103</td></tr><tr><td>In California's farm country, climate change is likely to trigger more pesticide use, fouling waterways</td><td>103</td></tr><tr><td>Vital soil organisms being harmed by pesticides, study shows</td><td>104</td></tr><tr><td>Interdiction des néonicotinoïdes : la justice de l'UE rejette un pourvoi de Bayer</td><td>104</td></tr></table>
Publication du 1er outil national de sélection des couples espèce/biomarqueur pour le suivi de la qualité des milieux aquatiques : enjeux & perspectives pour la communauté Ecotox

<figure><img src="image_3.png" /></figure>

<figure><img src="image_4.png" /></figure>

FONDATION
ROVALTAIN
SCIENCE & EQUITY

<figure><img src="image_5.png" /></figure>

GIP Seine-Aval

<figure><img src="image_6.png" /></figure>

OFB
DEPARTEMENT
DU MOODISTO

Le projet Biomarqueurs & Biodiversité : contexte et bilan

Réalisé entre 2017 et 2020 dans le cadre de l'appel à manifestations d'intérêt « Besoins de développements en appui à la surveillance et à l'évaluation de l'état des eaux et des milieux aquatiques » de l'OFB, le projet Biomarqueurs et Biodiversité (B&B) a eu pour objet :

1- De répertorier les couples espèce/biomarqueur disponibles sur le territoire national ;

2- D'évaluer la pertinence de l'utilisation des biomarqueurs pour la surveillance opérationnelle des masses d'eau continentales, côtières et de transition dans le contexte de la Directive Cadre sur l'Eau (DCE).

La Fondation ROVALTAIN a coordonné ce projet, en s'appuyant sur des partenaires et experts issus des organismes suivants : l'INRAE, l'UMR-I 2-SEBIO, le LIENS, l'EA MMS, l'UMR LEMAR, et le GIP Seine-Aval. Un travail d'enquête approfondi auprès de 15 laboratoires de recherche français a ainsi permis de référencer 331 couples espèce/biomarqueur, de les évaluer selon leur maturité opérationnelle et de caractériser leurs limites, leur spécificité et leur pertinence écologique dans un objectif de surveillance réglementaire des milieux aquatiques.

A émergé de ce travail une base de données (BMK) recensant les couples espèce/biomarqueur utilisables dans 4 contextes d'application différents : surveillance générale, qualité du rejet, impact du rejet sur le milieu, diagnostic control enquête ; et pour 3 types de masses d'eau : continentale, de transition, côtière. Un aperçu de l'utilisation de cette base de données est présenté en Annexe 1. Cet outil opérationnel est accompagné d'un guide pratique destiné aux utilisateurs.

Basée sur l'analyse de l'état chimique (45 molécules) et de l'état écologique des masses d'eau, la DCE prend peu en compte l'état biologique et physiologique des espèces animales et végétales présentes au sein de l'écosystème aquatique : des paramètres qui pourraient pourtant améliorer la description de l'état des milieux. Face à ce constat, de nombreux écotoxicologues œuvrent en faveur de l'intégration des
biomarqueurs et des bio-essais dans ce cadre réglementaire, à l'image de ce qui est déjà réalisé dans la Directive Cadre Stratégie pour le Milieu Marin (DCSMM). Afin d'évaluer les manques essentiels de la DCE, un consortium européen s'est également constitué et a mis en avant l'intérêt d'utiliser des méthodes innovantes de surveillance biologique, complémentaires à l'analyse chimique, appelées « Effect-based Methods » (EBMs), ou méthodes basées sur les effets (Wernersson et al., 2015). Ces EBMs, qui regroupent les biomarqueurs et les bio-essais, sont proposées en complément des analyses chimiques et écologiques.

Dans ce contexte, les biomarqueurs sont donc de véritables outils de détermination de l'état de santé des organismes aquatiques, à la fois pour la surveillance générale, mais aussi lors d'un incident isolé de pollution. Une réflexion autour de l'utilisation de ces outils dans le cadre réglementaire de la DCE a ainsi fait l'objet d'un premier article (Milinkovitch et al., 2019).

La pertinence et l'opérationnalité des couples espèce/biomarqueur au sein des quatre cadres d'études mentionnés précédemment ont fait l'objet d'un classement en fonction de 10 critères d'évaluation, comme l'existence d'une relation dose/réponse, l'existence de valeurs de base et de valeurs seuil ou encore des considérations technico-économiques.

Le projet B&B a permis de mettre en avant l'expertise française sur les biomarqueurs en écotoxicologie aquatique et de faire émerger la base de données BMK recensant les couples espèce/biomarqueur disponibles pour la biosurveillance aquatique. C'est le premier outil d'aide à la décision de cette envergure disponible en France.

## Une base de données des couples espèce/biomarqueur disponibles en fonction du cadre d'étude : pour qui ? Pour quoi faire ?

La base de données BMK a permis dans un premier temps de faire un état des lieux des biomarqueurs actuellement disponibles en France, et de rassembler la communauté autour de ce sujet. Cette base de données est la plus exhaustive réalisée à ce jour en France et a pour fonction (i) d'éclairer les gestionnaires de l'environnement sur le potentiel de ces outils dans un contexte opérationnel et (ii) d'accompagner et nourrir les thématiques de recherche en cours. Ainsi, les étudiants, chercheurs ou gestionnaires de l'eau souhaitant en savoir plus sur la possible utilisation d'un couple espèce/biomarqueur et son degré de maturité opérationnelle peuvent trouver les informations nécessaires (contacts, chercheurs...) sur le Guide Pratique associé à la BMK. Ce guide accompagne les utilisateurs dans la prise en main de cet outil et apporte des précisions sur les critères de sélection des couples espèce/biomarqueur ainsi que sur leur pertinence en fonction du contexte de l'étude, e.g. méthode active ou passive.

## Quelle stratégie pour l'intégration des couples espèce/biomarqueur dans un cadre réglementaire ? Au niveau national ? Au niveau européen ?

En juin 2020, après un examen approfondi, la commission européenne a finalement décidé de ne pas procéder à la révision de La DCE et de ne pas modifier les critères et méthodes d'évaluation en place. Le point positif de cette décision est que le texte ne sera pas affaibli. En revanche, l'intégration d'outils de biosurveillance aquatique tels que les biomarqueurs n'a pas été validée et la décision remise à la prochaine date de révision.

Ce délai pourra permettre d'affiner l'utilisation de ces biomarqueurs et de relever les défis qu'elle représente afin de s'insérer dans un cadre réglementaire, notamment :

- Définir de façon précise, une batterie de marqueurs capables de couvrir toutes les classes de produits chimiques qui peuvent affecter l'homéostasie des espèces aquatiques ;
- Mettre en place des indicateurs (de type Effect-Based Threshold « EBT ») permettant de définir des valeurs de base et des valeurs seuils, afin de différencier une qualité d'eau acceptable d'une qualité d'eau médiocre.

En termes de stratégies de déploiement, l'inventaire des couples espèce/biomarqueur réalisé grâce à ce projet pourrait ainsi être un premier pas vers la mise en place de protocoles de surveillance cadrés impliquant les différents acteurs de l'eau à un niveau national, au premier rang desquels les agences et gestionnaires de l'eau. Pour cela, il est nécessaire d'identifier des sites pilotes et travailler de concert avec les acteurs de l'eau, afin de lever les interrogations sur le mode d'application, la pertinence des biomarqueurs (valeurs de base, valeurs seuils ...) et leurs apports pour la définition d'actions. En France, on dénombre déjà plusieurs exemples de déploiement de ces couples espèce/biomarqueur pour la biosurveillance des masses d'eau. Parmi eux, le projet BIOSURVEILLANCE<fnref n="1" /> (2019 - 2022), un projet pilote coordonné par le GIP Seine-Aval et basé sur l'utilisation de biomarqueurs pour un appui à la surveillance de la qualité de différents types de masses d'eau du district Seine-Normandie, en concertation avec l'agence de l'eau Seine-Normandie. Plus précisément, des biomarqueurs de génotoxicité et d'immunotoxicité sont mesurés sur organismes encagés, représentatifs de différents milieux (eaux douce, de transition, et marin côtier) et de différents groupes taxonomiques de sensibilités différentes aux contaminants chimiques (crustacés, bivalves et poissons).

Il apparaît donc à la fois nécessaire de présenter des exemples de suivi fonctionnels et de mettre en place des groupes de travail, pilotés par les gestionnaires et accompagnés par les chercheurs, afin de définir des « protocoles types » concrets, adaptés et transférables, à l'image du projet SASHIMI<fnref n="2" />, en cours de réalisation dans le bassin Seine-Normandie.

Enfin, en parallèle du projet B&B, un autre groupe de travail a été réuni par l'OFB et l'INERIS dans le cadre du partenariat avec le consortium AQUAREF<fnref n="3" /> afin de lister les bio-essais disponibles sur le territoire national. Les résultats de ce groupe de travail, complémentaires à ceux du projet B&B, seront publiés courant 2021, et la mise en commun de ces deux études constituera une base de travail solide. Déjà amorcée, la réflexion autour du déploiement de méthodes intégratives permettant de combiner plusieurs bio-essais ou plusieurs biomarqueurs pour la biosurveillance des milieux aquatiques est plus que jamais d'actualité. Le concept des EBMs (Wernersson et al., 2015) a permis d'esquisser des réflexions et des recommandations par des groupes de travail au niveau européen sur leur intégration au niveau de la DCE ou dans des contextes d'études locales ou de monitoring spécifique. Parmi ces groupes de travail, le projet collaboratif SOLUTIONS<fnref n="4" /> recommande l'utilisation d'EBMs intégratives pour le diagnostic et la biosurveillance de la qualité du milieu aquatique, avec le déploiement d'une batterie minimum de bio-essais *in vitro* et *in vivo* (Brack et al., 2019).

Concernant plus spécifiquement les biomarqueurs, une réflexion autour des nouvelles méthodes intégratives permettant d'avoir en sortie une réponse biologique lisible par les gestionnaires et les décisionnaires est

<footnote n="1">¹ https://www.seine-aval.fr/projet/biosurveillance/</footnote>

<footnote n="2">² https://www.seine-aval.fr/projet/sashimi/ Projet de Surveillance Active de l'impact de la preSSion cHIMIque par des biomarqueurs (SASHIMI)</footnote>

<footnote n="3">³ AQUAREF, laboratoire national de référence pour la surveillance des milieux aquatiques, est né de la nécessité de renforcer l'expertise française dans le domaine de la surveillance des milieux aquatiques à partir de la mise en réseau des compétences et des capacités de recherche des cinq établissements publics directement concernés: BRGM, IFREMER, INERIS, INRAE, LNE.</footnote>

<footnote n="4">⁴ https://www.solutions-project.eu/ SOLUTIONS recherche des outils, méthodes et modèles nouveaux et performants pour soutenir les décisions en matière de politiques environnementales et de l'eau</footnote>
nécessaire. A ce sujet, un deuxième article ayant pour objet la comparaison et l'analyse critique de ces différentes méthodes est en cours de rédaction par les partenaires du projet B&B et la Fondation Rovaltain.

## Quelle évolution pour la base de données des couples espèce/biomarqueur ? Faire vivre/évoluer la base de données ?

La base de données Biomarqueurs & Biodiversité recense les couples espèce/biomarqueur disponibles pour contribuer à évaluer la qualité des masses d'eau à un instant précis. Bien que cette base de données soit le 1er outil de cette envergure créé pour la biosurveillance des écosystèmes aquatiques en France, il n'est pas exhaustif. En effet, de nouveaux couples espèce/biomarqueur ont pu être développés depuis la publication de cette base de données. Par ailleurs, parmi les couples espèce/biomarqueur sélectionnés, la maturité de certains a pu évoluer entre la publication de la base de données et la rédaction de cette tribune.

Il apparaît donc nécessaire de faire évoluer cet outil, dans le but d'intégrer de nouveaux couples espèce/biomarqueur, mais également afin de prendre en compte la modulation de certains paramètres, comme le degré de maturité opérationnelle, les valeurs de base/seuils ou encore un changement de protocole permettant de faciliter l'utilisation d'un biomarqueur.

Plusieurs solutions sont à l'étude :

- La mise à jour annuelle sur le site web du projet par la Fondation Rovaltain, avec l'appui des partenaires ayant contribués à la création de la base de données, après validation par un comité scientifique ;

- Une journée annuelle organisée à la Fondation Rovaltain dédiée aux biomarqueurs et permettant de faire remonter les informations principales sur les nouveaux biomarqueurs.

## Comment fédérer la communauté Ecotox autour de l'outil de sélection des couples Espèce/Biomarqueur au niveau national ?

Le projet B&B et la création de la base de données BMK et du Guide Pratique associé constituent une opportunité de fédérer la communauté Ecotox autour du sujet transverse des biomarqueurs. Pour faire suite à ce projet, et en coordination avec les résultats du groupe de travail bio-essais, des formations sur l'utilisation des biomarqueurs et des bio-essais en fonction des différents cadre d'étude pourraient être mises en place/organisées par la Fondation Rovaltain. Il pourrait ainsi être envisagé une école d'été/d'hiver dédiée à l'apprentissage de l'utilisation des biomarqueurs et des bio-essais.

## Contacts

Damien Baudiffier, Fondation Rovaltain
[d.baudiffier@fcsrovaltain.org](mailto:d.baudiffier@fcsrovaltain.org)

Delphine Delaunay, Fondation Rovaltain
[d.delaunay@fcsrovaltain.org](mailto:d.delaunay@fcsrovaltain.org)

<figure><img src="image_7.png" /></figure>

FONDATION
ROVALTAIN
SCIENCE & ÉQUILIBRE

Remerciements : la Fondation Rovaltain remercie Olivier Perceval (OFB), Cédric Fisson (GIP Seine-Aval) et Wilfried Sanchez (IFREMER) pour la relecture de ce document.
# Pour en savoir plus et obtenir la Base de Données BMK & le Guide Pratique

https://sites.google.com/view/biomarqueur-et-biodiversite/accueil

## Bibliographie citée

Brack, W., Aissa, S.A., Backhaus, T. et al., 2019. Effect-based methods are key. The European Collaborative Project SOLUTIONS recommends integrating effect-based methods for diagnosis and monitoring of water quality. Environ. Sci. Eur. 31, 10.

Milinkovitch T, Geffard O, Geffard A, Mouneyrac C, Chaumot A, Xuereb B, Fisson C, Minier C, Auffret M, Perceval O, Egea E, Sanchez W., 2019. Biomarkers as tools for monitoring within the Water Framework Directive context: concept, opinions and advancement of expertise. Environ Sci. Pollut. Res. Int. 26(32), 32759-32763.

Wernersson, A.-S., Carere, M., Maggi, C., Tusil, P., Soldan, P., James, A., Sanchez, W., Dulio, V., Broeg, K., Reifferscheid, G., Buchinger, S., Maas, H., Van Der Grinten, E., O'Toole, S., Ausili, A., Manfra, L., Marziali, L., Polesello, S., Lacchetti, I., ... Kase, R., 2015. The European technical report on aquatic effect-based monitoring tools under the water framework directive. Environ. Sci. Eur. 27(1), 7.

## Annexe 1. Exemple d'utilisation de la Base de données BMK : Rang de maturité opérationnelle des couple espèce/biomarqueur en fonction du Cadre d'étude (entouré en rouge, dans l'exemple, la surveillance générale) et du milieu (entouré en vert, dans l'exemple, tout milieu confondu)

<figure><img src="image_8.png" /></figure>
ERA / PUBLICATIONS

SCIENTIFIQUES /

COMMUNAUTES

MICROBIENNES

AQUATIQUES

Study of the Tagus River and Entrepenas reservoir ecosystem around the Trillo nuclear power plant using chemometric analysis: Influence on water, sediments, algae and fish

Authors: Arguelles R, Toledo M, Martin MA

Source: CHEMOSPHERE 279:130532, 2021, DOI: 10.1016/j.chemosphere.2021.130532

**Abstract:** The fluvial and aquatic ecosystem of the Tagus River and Entrepenas reservoir located in the surrounding of the Trillo nuclear power plant (NPP) was evaluated from 1992 to 2008, considering the physicalchemical characteristics, metal content and radiological activity of the water, sediments, algae and fish...

The link between pharmaceuticals and cyanobacteria: a review regarding ecotoxicological, ecological, and sanitary aspects

Authors: Costa RD, Quadra GR, Souza HD et al.

Source: ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH Early Access, 2021, DOI: 10.1007/s11356-021-14698-5

**Abstract:** Cyanobacteria are important for ecosystem functioning, but eutrophication may affect the surrounding biome by losing ecosystem services and/or through affecting the cyanotoxins production that threatens ecological and human health. Pollution is an environmental issue that affects aquatic ecosystems worldwide, and the knowledge of the role of synthetic chemicals such as pharmaceuticals is still scarce. [...] An overview of ecotoxicological tests performed in the literature exposing cyanobacteria to pharmaceuticals and the possible consequences regarding ecological and sanitary aspects was conducted...

Insights into the microbial
degradation and biochemical
mechanisms of carbamates

Authors: Mishra S, Pang SM, Zhang WP et al.

Source: CHEMOSPHERE 279:130500, 2021, DOI: 10.1016/j.chemosphere.2021.130500

**Abstract:** [...] This review discusses the deep understanding of carbamate degradation mechanisms with microbial strains, metabolic pathways, molecular mechanisms, and their genetic basis in degradation.

A trophic transfer study:
accumulation of multi-walled
carbon nanotubes associated
to green algae in water flea
*Daphnia magna*

Authors: Politowski I, Wittmers F, Hennig MP et al.
**Source:** NANOIMPACT 22:100303, 2021 DOI: 10.1016/j.impact.2021.100303

**Abstract:** Carbon nanotubes (CNT) are promising nanomaterials in modern nanotechnology and their use in many different applications leads to an inevitable release into the aquatic environment. In this study, we quantified trophic transfer of weathered multi-walled carbon nanotubes (wMWCNT) from green algae to primary consumer Daphnia magna in a concentration of 100 µg L-1 using radioactive labeling of the carbon backbone (C-14-wMWCNT). Trophic transfer of wMWCNT was compared to the uptake by daphnids exposed to nanomaterials in the water phase without algae...

## Thorium Exposure Drives Fatty Acid and Metal Transfer from Biofilms to the Grazer *Lymnaea* sp.

**Authors:** Doose C, Fadhlaoui M, Morin S, Fortin C

**Source:** ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY Early Access, DOI:10.1002/etc.5067

**Abstract:** Aquatic ecotoxicological risks associated with tetravalent metallic elements such as thorium (Th) are still poorly understood. Periphytic biofilm represents an important food Source: in aquatic environments; thus, such risks could severely affect nutrient and energy cycling in these ecosystems. The present study investigated the potential for Th to change the fatty acid composition of biofilm communities. Bioaccumulation of Th and fatty acids were measured after 4 wk to 2 exposure conditions: a control (C0) and Th exposure (C10)...

## Cytotoxicity and genotoxicity of coated-gold nanoparticles on freshwater algae *Pseudokirchneriella* *subcapitata*

**Authors:** Mahaye N, Leareng SK, Musee N

**Source:** AQUATIC TOXICOLOGY 236:105865, 2021, DOI: 10.1016/j.aquatox.2021.105865

**Abstract:** Gold engineered nanoparticles (nAu) are increasingly detected in ecosystems, and this raises the need to establish their potential effects on aquatic organisms. Herein, cytotoxic and genotoxic effects of branched polyethylenimine- and citrate-coated nAu (5, 20, and 40 nm) on algae *Pseudokirchneriella subcapitata* were evaluated. The apical biological endpoints: growth inhibition and chlorophyll a content were investigated at 62.5-1000 µg/L over 168 h. In addition, the apurinic/apyrimidinic sites, randomly amplified polymorphic deoxyribonucleic acid profiles, and genomic template stability were assessed to determine the genotoxic effects of nAu...

## Exploring the role of water chemistry on metal accumulation in biofilms from streams in mining areas

**Authors:** Laderriere V, Le Faucheur S, Fortin C

**Source:** SCIENCE OF THE TOTAL ENVIRONMENT 784:146986, 2021. DOI: 10.1016/j.scitotenv.2021.146986

**Abstract:** Biofilms play a key role in aquatic ecosystems. They are ubiquitous, even in the most contaminated ecosystems, and have great potential as biomonitors of exposure to contaminants such as metals. Freshwater biofilms and surface waters were sampled in two active mining areas of Canada: in the northern part of Nunavik (Quebec) and in the Greater Sudbury area (Ontario)...

## Toxicity evaluation of nano-TiO2 in the presence of
functionalized microplastics at two trophic levels: Algae and crustaceans

**Authors:** Thiagarajan V, Alex SA, Seenivasan R et al.

**Source:** SCIENCE OF THE TOTAL ENVIRONMENT
784:147262, 2021, DOI: 10.1016/j.scitotenv.2021.147262

**Abstract:** The rising use of contaminants such as nanoparticles and microplastics has taken a heavy toll on the marine environment. However, their combined toxic effects on the species across various trophic levels remain quite unexplored. The aim of this study was to explore the effects of three surface-functionalized (carboxylated, plain, and aminated) polystyrene microplastics on nano-TiO2 toxicity across two trophic levels containing Chlorella sp. as the prey and Artemia salina as the predator. The experiments carried out on Chlorella sp. include the toxicity assessment, oxidative stress determination, and uptake of nano-TiO2 (both in the presence and absence of microplastics)...

Metagenomic analysis of a mega-city river network reveals microbial compositional heterogeneity among urban and peri-urban river stretch

**Authors:** Yadav R, Rajput V, Dharne M

**Source:** SCIENCE OF THE TOTAL ENVIRONMENT
783:146960, 2021, DOI:10.1016/j.scitotenv.2021.146960

**Abstract:** The rivers in the megacities face a constant inflow of extremely polluted wastewaters from various Source:s, and their influence on the connected peri-urban river is still poorly understood. The riverine system in Pune consists of Rivers Mula, Ramnadi, Pawana, Mutha,

and Mula-Mutha, traversing through the urban settlements of Pune before joining River Bhima in the peri-urban region. We used MinION-based metagenomic sequencing to generate a comprehensive understanding of the microbial diversity differences between the urban and peri-urban zones, which has not been explored at the meta scale until date...

Characterizations of heavy metal contamination, microbial community, and resistance genes in a tailing of the largest copper mine in China

**Authors:** Jiang XW, Liu WH, Xu H et al.

**Source:** ENVIRONMENTAL POLLUTION 280:116947, 2021, DOI: 10.1016/j.envpol.2021.116947

**Abstract:** Copper mine tailings are causing great environmental concern nowadays due to their high contents of heavy metals. These hazards may release to air, water, and soil, posing great threat to the living organisms in the surroundings. In the present work, we profiled the heavy metal contents, microbiome and resistome of a mine tailing in Dexing Copper Mine, which is the largest open-pit copper mine in China...

Effects of acid drainage from abandoned coal mines on the microbial community of Shandi River sediment, Shanxi Province

**Authors:** Chen D, Feng QY, Li WB and more...

**Source:** INTERNATIONAL JOURNAL OF COAL SCIENCE & TECHNOLOGY2021, DOI: 10.1007/s40789-021-00433-5
**Abstract:** The discharge of acid mine drainage from abandoned high sulfur (S) coal mines has caused serious pollution in the Shandi River, Yangquan, Shanxi Province. To determine the impact of long-term acid mine drainage on the microorganisms in the river, we collected river sediments from a polluted tributary (Group P) and the mainstream of Shandi River (Group R) to study the bacterial diversity and community composition...

# The impacts of metal-based engineered nanomaterial mixtures on microbial systems: A review

**Authors:** Wu SS, Gaillard JF, Gray KA

**Source:** SCIENCE OF THE TOTAL ENVIRONMENT
780:146496, 2021, DOI: 10.1016/j.scitotenv.2021.146496

**Abstract:** The last decade has witnessed tremendous growth in the commercial use of metal-based engineered nanomaterials (ENMs) for a wide range of products and processes. Consequently, direct and indirect release into environmental systems may no longer be considered negligible or insignificant. Previous research has focused primarily on the acute effects of individual ENMs using pure cultures under controlled laboratory environments, which may not accurately reveal the ecological impacts of ENMs under real environmental conditions. The goal of this review is to assess our current understanding of ENM effects as we move from exposure of single to multiple ENMs or microbial species. For instance, are ENMs' impacts on microbial communities predicted by their intrinsic physical or chemical characteristics or their effects on single microbial populations; how do chronic ENM interactions compare to acute toxicity; does behavior under simplified laboratory conditions reflect that in environmental media; finally, is biological stress modified by interactions in ENM mixtures relative to that of individual ENM? ...

# Effect of pyrethroid on growth and protein content of two unicellular cyanobacteria (blue green algae)

**Authors:** Bano S, Shafique S, Burhan ZUN, Rasheed M

**Source:** PAKISTAN JOURNAL OF BOTANY 53(4): 1531-1535, 2021, DOI: 10.30848/PJB2021-4(24)

**Abstract:** Two unicellular strains of cyanobacteria Chroococcidiopsis sp. and Microcystis sp. isolated from marine environment were mass cultured and their interaction with pyrethroid pesticides was studied...

# Dissolved organic matter modulates the impact of herbicides on a freshwater alga: A laboratory study of a three-way interaction

**Authors:** Morin S, Coquille N, Eon M et al.

**Source:** SCIENCE OF THE TOTAL ENVIRONMENT
782:146881, 2021, DOI: 10.1016/j.scitotenv.2021.146881

**Abstract:** In freshwater environments, microorganisms such as microalgae are influenced by the concentrations of dissolved chemicals but can modify the fate of these substances by biosorption, accumulation and even metabolization. In this laboratory study, we assessed the growth and physiology of non-axenic cultures of the chlorophyte Sphaerellopsis sp. exposed to environmental concentrations of diuron, irgarol and S-metolachlor (0.5, 0.5 and 5 µg.L-1, respectively) singly and in mixture, in the presence or absence of natural dissolved organic matter. The growth, photosynthetic efficiency and relative intracellular lipid content of Sphaerellopsis sp., as were measured after 14
days of exposure, as were the concentrations of bacteria in the cultures...

## Environmental concentrations of sulfonamides can alter bacterial structure and induce diatom deformities in freshwater biofilm communities

**Authors:** Kergoat L, Besse-Hoggan P, Leremboure M et al.

**Source:** FRONTIERS IN MICROBIOLOGY 12:643719, 2021, DOI: 10.3389/fmicb.2021.643719

**Abstract:** The antibiotics commonly found in surface waters include sulfamethoxazole (SMX) and sulfamethazine (SMZ), which belong to the class of sulfonamides, the oldest antibiotic class still in use. [...] Sulfonamides are known to inhibit bacterial growth by altering microbial production of folic acid, but sub-lethal doses may trigger antimicrobial resistance, with unknown consequences for exposed microbial communities. We investigated the effects of two environmentally relevant concentrations (500 and 5,000 ng L-1) of SMZ and SMX on microbial activity and structure of periphytic biofilms in stream mesocosms for 28 days...

## The inhibitory effects of the antifouling compound Irgarol 1051 on the marine diatom Skeletonema sp. across a broad range of photosynthetically active radiation

**Authors:** Tang Y, Chen C, Sheng YJ et al.

**Source:** ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH Early Access, 2021, DOI: 10.1007/s11356-021-14135-7

**Abstract:** The release of anthropogenic organic pollutants has resulted in extensive environmental risks to coastal waters. Among pollutants released, the most common antifoulant, Irgarol 1051, is an effective inhibitor of photosystem II of photoautotrophs; thus, the continuous release of this compound into surrounding seawater would potentially threaten marine algae. To investigate this, we grew the model marine diatom Skeletonema sp. at different concentrations of Irgarol 1051 and levels of photosynthetically active radiation (PAR)...

## The microbial community in a green turtle nesting beach in the Mediterranean: application of the Biolog EcoPlate approach for beach pollution

**Authors:** Candan ED, Idil N, Candan O

**Source:** ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH Early Access, 2021, DOI: 10.1007/s11356-021-14196-8

**Abstract:** This study aims to characterize the microbial community and its relationship with heavy metal pollution in the beaches of Sugozu, an important nesting site for the green turtle. Heavy metal concentrations of sand samples from subregions of Sugozu were determined using ICP-MS. The microbial community was analyzed using the Biolog (R) EcoPlate. The relationship between microbial catalytic activity and heavy metal levels were analyzed using canonical correspondence analysis...

## Combined effects of sulfamethoxazole and erythromycin on a freshwater
microalga, *Raphidocelis subcapitata*: toxicity and oxidative stress

**Authors:** Zhang YB, He D, Chang F et al.

**Source:** ANTIBIOTICS-BASEL 10:576, 2021, DOI: 10.3390/antibiotics10050576

**Abstract:** This study investigated the environmental effects of two familiar emerging contaminants, sulfamethoxazole and erythromycin, and their mixture (10:1 w/w) using a green microalga, *R. subcapitata*. The cell density, pigment content, and the activities of superoxide dismutase, catalase, glutathione, glutathione peroxidase, and glutathione S-transferase were analysed...

Ecological risk potentials of petroleum hydrocarbons and heavy metals shape the bacterial communities of marine hydrosphere at Atlantic Ocean, Atlas Cove, Nigeria

**Authors:** Oyetibo GO, Ige OO, Obinani PK, Amund OO

**Source:** JOURNAL OF ENVIRONMENTAL MANAGEMENT 289:112563, 2021, DOI: 10.1016/j.jenvman.2021.112563

**Abstract:** Trans-Atlantic voyage of petroleum often leads to marine pollution with petroleum hydrocarbons and heavy metals that defines structures of autochthonous bacteria in the hydrosphere. Bacterial taxa of marine sediments exposed to petroleum transport activities were profiled using 16S rDNA metagenomics and correlated with the geochemistry to establish their impact on the microbiome...

Biodiversity and functional trait effects on copper toxicity in a proof-of-concept multispecies microalgal assay

**Authors:** Joonas E, Olli K, Kahru A, Aruoja V

**Source:** ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 55:102204, 2021, DOI: 10.1016/j.algal.2021.102204

**Abstract:** This study aimed to elucidate biodiversity effects on algal biomass production under toxicant stress and to increase environmental realism in ecotoxicological testing by exposing artificial algal communities to Cu2+. All 15 possible combinations of four functionally distinct microalgal species (green algae Chlamydomonas reinhardtii and *Raphidocelis subcapitata*, diatom *Fistulifera pelliculosa* and cyanobacterium Synechocystis sp.) were exposed to 0.01 and 0.02 mg Cu2+/L in a modified algal growth inhibition assay (OECD 201)...

Formation of silver nanoparticles in aquatic environments facilitated by algal extracellular polymeric substances: Importance of chloride ions and light

**Authors:** Xiong SC, Cao XS, Fang H et al.

**Source:** SCIENCE OF THE TOTAL ENVIRONMENT 775:145867, 2021, DOI: 10.1016/j.scitotenv.2021.145867

**Abstract:** Natural conversion of metal species is an important Source: for nanoscale metal particles in the aquatic environment, and it could affect their fate and toxicity. Extracellular polymeric substances (EPSs) are ubiquitous and abundant in the aquatic environment, thus likely can reduce metal ions to nanoscale particles. However, the effect of natural inorganic ligand and light on this process has not been well
investigated. In this work, Ag+ was readily reduced to silver nanoparticles (AgNPs, around 15 nm in size) by the EPS collected from *Chlorella pyrenoidosa...*

## Evaluation of Multivariate Biomarker Indexes Application in Ecotoxicity Tests with Marine Diatoms Exposed to Emerging Contaminants

**Authors:** Pires VL, Novais SC, Lemos MFL et al.

**Source:** APPLIED SCIENCES-BASEL 11:3878, 2021, DOI: 10.3390/app11093878

**Abstract:** Worldwide anthropogenic activities result in the production and release of potentially damaging toxic pollutants into ecosystems, thereby jeopardizing their health and continuity. Research studies and biomonitoring programs attend to this emerging problematic by applying and developing statistically relevant indexes that integrate complex biomarker response data to provide a holistic approach, reflecting toxically induced alterations at the organism or population level. Ultimately, indexes allow simple result communications, enhancing policy makers understanding, and contributing to better reSource: and environmental managing policies. In this study three indexes, the integrated biomarker response index (IBR), the bioeffects assessment index (BAI) and principal components analysis (PCA), were evaluated for their sensitivity in revealing toxically induced stress patterns in cells of the diatom *Phaeodactylum tricornutum* under contaminant exposure...

## Response of Benthic Diatom Assemblages to

## Contamination by Metals in a Marine Environment

**Authors:** Martinez YJ, Siqueiros-Beltrones DA, Marmolejo-Rodriguez AJ

**Source:** JOURNAL OF MARINE SCIENCE AND ENGINEERING 9:443, 2021, DOI: 10.3390/jmse9040443

**Abstract:** Studies on marine benthic diatoms in environments contaminated by metals are scarce. The typical structure of benthic diatom assemblages (species richness, diversity, dominance, dominant taxa) from undisturbed environments may be used as reference for contrasting with contaminated environments in order to observe how said assemblages respond to such disturbance. [...] To do this, concentrations of 24 metals were surveyed in a coastal zone impacted by mining residues, and the structure of benthic local diatom assemblages was described...

## Glyphosate-based herbicide exposure affects diatom community development in natural biofilms

**Authors:** Corrales N, Meerhoff M, Antoniades D

**Source:** ENVIRONMENTAL POLLUTION 284:117354, 2021, DOI: 10.1016/j.envpol.2021.117354

**Abstract:** Glyphosate herbicide is ubiquitously used in agriculture and weed control. It has now been identified in aquatic ecosystems worldwide, where numerous studies have suggested that it may have both suppressive and stimulatory effects on diverse non-target organisms. We cultured natural biofilms from a hypereutrophic environment to test the effects on periphytic diatoms of exposure to a glyphosate-based herbicide formulation at concentrations from 0 to 10 mg L-1 of active ingredient...

## Influence of silver nanoparticle-based coating
# on calcareous rock surfaces on microbial biofilm colonization in intertidal environments in Campeche, Mexico

**Authors:** Camacho-Chab JC, Ortega-Morales BO, Gaylarde C et al.

**Source:** WATER AIR AND SOIL POLLUTION 232:172, 2021, DOI: 10.1007/s11270-021-05123-1

**Abstract:** The influence of silver nanoparticles (AgNPs) on microbial biofilm colonization on natural calcareous rocks was studied in the Campeche marine intertidal environment in southeast Mexico. The biofilm formation was assessed by color spectrophotometer measurements represented in the CIELAB color space, digital image analysis, confocal laser scanning microscopy, biochemical biomarkers, and enumeration of morphologically characterized cells by FlowCAM (R). Coupons were exposed with or without polydimethylsiloxane (PDMS) coatings and without or with various concentrations of AgNPs...

# The effect of chromium on photosynthesis and lipid accumulation in two chlorophyte microalgae

**Authors:** Bashir KMI, Lee HJ, Mansoor S et al.

**Source:** ENERGIES 14:2260, 2021, DOI: 10.3390/en14082260

**Abstract:** Heavy metals have adverse effects on microalgae metabolism and growth. Photosynthesis and lipid profile are quite sensitive to heavy metal toxicity. The impact of hexavalent chromium-Cr(VI) on photosynthesis and lipid accumulation in Mucidosphaerium pulchellum and Micractinium pusillum exposed to different concentrations (0-500 µg L-1) was investigated for 11 days...

# Toxicity of copper on marine diatoms, *Chaetoceros calcitrans* and *Nitzchia closterium* from Cochin estuary, India

**Authors:** Neethu KV, Saranya KS, Krishna NGA et al.

**Source:** ECOTOXICOLOGY Early Access, 2021, DOI: 10.1007/s10646-021-02410-9

**Abstract:** The effects of copper (Cu) toxicity on the growth, pigments, protein, carbohydrate, lipid and antioxidant enzyme activities of two endemic microalgae, Chaetoceros calcitrans and Nitzchia closterium from Cochin estuary were studied and compared...

# Bacteria compete with hematite nanoparticles during their uptake by the ciliate *Tetrahymena thermophila*

**Authors:** Guo WB, Yang LY, Miao AJ

**Source:** JOURNAL OF HAZARDOUS MATERIALS 411:125098, 2021, DOI: 10.1016/j.jhazmat.2021.125098

**Abstract:** Bacterial accumulation of engineered nanoparticles (NPs) result in their transfer along the food chain. However, there are a lot of NPs not associated with bacteria. Whether bacteria, as representative biotic particles, influence the biological uptake of these non-associated NPs in aquatic ecosystems is unclear. In the present study, we examined the effects of four bacterial species on the uptake kinetics of polyacrylate-coated hematite nano particles (HemNPs) by the ciliate Tetrahymena thermophila...
# Pesticide mixture toxicity to algae in agricultural streams? Field observations and laboratory studies with in situ samples and reconstituted water

**Authors:** Stenstrom JR, Kreuger J, Goedkoop W

**Source:** ECOTOXICOLOGY AND ENVIRONMENTAL

SAFETY 215:112153, 2021, DOI:

10.1016/j.ecoenv.2021.112153

**Abstract:** Long-term pesticide water concentrations were investigated in four agricultural streams and their mixture toxicity on algae was assessed, based on realistic (i.e. observed) concentrations in laboratory tests using (i) natural weekly water samples and (ii) reconstituted pesticide-spiked water samples representing mixtures with predicted high mixture. This approach both covered the full complexity of natural water samples and the controlled approach of reconstituted water samples...

ERA / PUBLICATIONS

SCIENTIFIQUES /

ECOTOXICOLOGIE

SPATIALE

# Detecting landscape scale consequences of insecticide use on invertebrate communities

**Authors:** Mancini F, Woodcock BA, Redhead J, Spurgeon DJ et al.

**Source:** *The Future of Agricultural Landscapes, Part I.* Advances in Ecological Research 63:93-126, 2020, DOI: 10.1016/bs.aecr.2020.07.001

**Abstract:** Insecticides represent an important management tool in agriculture. They provide a low cost and efficacious approach to pest control, where they may be employed both reactively and pre-emptively. It is likely insecticides will continue to play a role in meeting the challenge of feeding a growing global population. The widespread use of insecticides creates potential for negative impacts on non-target invertebrate populations. Many countries have regulatory processes in place to manage these. However, there regulatory processes have potential limitations when it comes to detecting the long-term and large-scale consequences of insecticide use. For example, long-term sub-lethal effects resulting from low dose exposure durations are rarely considered in toxicity tests, while predictable additive effects of mixtures of active ingredient are often ignored. In order to address this data gap, there is substantial scope for using existing spatially and temporally explicit biological records of species occurrence. This could help in monitoring and assessment to support our responsibilities to maintain biodiversity while continuing to grow enough food to feed the human population. How this is achieved poses several problems, both practical and analytical. Here, we describe how national-scale data on the spatial and temporal distribution of native invertebrates can be combined with maps of large-scale insecticide application and exposure risk to explore the actual consequences for non-target native biodiversity. Understanding these impacts on native invertebrates will provide a vital evidence base to inform policy decisions that could complement existing regulatory processes.
## Neonicotinoid residues in honey from urban and rural environments

**Authors:** Kavanagh S, Henry M, Stout JC, White B

**Source:** ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH, Early Access, 2021, DOI: 10.1007/s11356-021-12564-y

**Abstract:** Pesticide residues in honey can negatively affect bee health. Although recent studies have detected neonicotinoid residues in honeys from around the world, little is known about how residues relate to land use and vegetation composition. To investigate potential relationships, we sampled multi-floral honey from 30 Apis mellifera hives from urban, agricultural and semi-natural habitats (SNH), identified and quantified three neonicotinoids present (clothianidin, imidacloprid and thiacloprid) using UHPLC-MS, and classified surrounding land use up to 5 km around hive sites.

## Occurrence, spatial distribution, Source, and ecological risk assessment of organochlorine pesticides in Dongting Lake, China

**Authors:** Cao, F., Li, Z., He, Q. et al.

**Source:** ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH, Early Access, 2021, DOI: 10.1007/s11356-021-12743-x

**Abstract:** The occurrence, distribution, Source:s, and ecological risks of organochlorine pesticides in Dongting Lake of China were investigated.

## Landscape Composition and Fungicide Exposure Influence Host-Pathogen Dynamics in a Solitary Bee

**Authors:** Krichilsky E, Centrella M, Eitzer B. et al.

**Source:** ENVIRONMENTAL ENTOMOLOGY 50(1):107-116, 2021, DOI: 10.1093/ee/nvaa138

**Abstract:** Both ecosystem function and agricultural productivity depend on services provided by bees; these services are at risk from bee declines which have been linked to land use change, pesticide exposure, and pathogens. Although these stressors often co-occur in agroecosystems, a majority of pollinator health studies have focused on these factors in isolation, therefore limiting our ability to make informed policy and management decisions. Here, we investigate the combined impact of altered landscape composition and fungicide exposure on the prevalence of chalkbrood disease, caused by fungi in the genus Ascosphaera Olive and Spiltoir 1955 (Ascosphaeraceae: Onygenales), in the introduced solitary bee, Osmia cornifrons (Radoszkowski 1887) (Megachilidae: Hymenoptera). We used both field studies and laboratory assays to evaluate the potential for interactions between altered landscape composition, fungicide exposure, and Ascosphaera on *O. cornifrons* mortality.

## Synergism between local- and landscape-level pesticides reduces wild bee floral visitation in pollinator-dependent crops

**Authors:** Bloom EH, Wood TJ, Hung KLJ, Ternest JJ et al.

**Source:** JOURNAL OF APPLIED ECOLOGY, Early Access, 2021, DOI: 10.1111/1365-2664.13871

**Abstract:** The hazard pesticides pose to pollinators are well-understood from laboratory studies. However, the field-level response of pollinators to pesticide use in agroecosystems is not well-established, nor is it clear if synergisms between pesticides affect pollinator visitation to crops...
Here, we evaluated if fungicide and insecticide use posed a hazard to wild and honeybees at 87 cucurbit-pumpkin, cucumber, watermelon-farms in the Midwestern United States. We also evaluated if synergisms between local- (i.e. focal cucurbit field) and landscape-level (i.e. surrounding crops) pesticide use influence wild and honeybee visitation to crop flowers.

ERA / PUBLICATIONS

SCIENTIFIQUES /

MICROBIOLOGIE ET

CONTAMINANTS

Metagenomic sequencing reveals detoxifying and tolerant functional genes in predominant bacteria assist *Metaphire guillelmi* adapt to soil vanadium exposure

Authors: Wang XW, Xia R, Sun MM, Hu F

Source: JOURNAL OF HAZARDOUS MATERIALS 415, 2021, DOI: 10.1016/j.jhazmat.2021.125666

**Abstract:** Due to extensive vanadium (V) mining and processing, an increasing amount of V has accumulated in soil, which poses a threat to public health. Consequently, we used earthworm (*Metaphire guillelmi*) incubation trials in Vcontaminated soil (0 & ndash;300 mg kg-1) to explore the response of soil indigenous bacteria and earthworm intestinal bacteria to V stress ...

Effects of increasing concentrations of fungicide Quadris(R) on bacterial

functional profiling in loamy sand soil

Authors: Aleksova M, Kenarova A, Boteva S and more...

Source: ARCHIVES OF MICROBIOLOGY, 2021, DOI: 10.1007/s00203-021-02423-2

**Abstract:** A mesocosm experiment was conducted to assess the side effects of the fungicide Quadris(R) on soil bacterial functioning. Quadris(R) was applied to a loamy sand soil at increasing concentrations (0.0-35.0 mg kg (-1) dry soil) calculated according to its active ingredient azoxystrobin (Az)...

Determination of Bactericidal Effects of Some Pesticides on Useful and Pathogenic Bacteria

Authors: Kotan R, Tozlu E

Source: JOURNAL OF TEKIRDAG AGRICULTURE FACULTY-TEKIRDAG ZIRAAT FAKULTESİ DERGİSİ 18(2): 197-212, 2021, DOI: 10.33462/jotaf.737039

**Abstract:** The damage of chemicals that are used for plant protection and plant nutrition products used in agriculture to human health and the environment is understood better day by day. However, a significant part of this damage occurs on the beneficial microbial fauna on the plant and in the soil described as a living system...

Field-scale studies on the change of soil microbial community structure and functions after stabilization at a chromium-contaminated site

Authors: Li DN, Li GH, Zhang DY
**Source:** JOURNAL OF HAZARDOUS MATERIALS 415, 2021, DOI: 10.1016/j.jhazmat.2021.125727

**Abstract:** Various remediation strategies have been developed to eliminate soil chromium (Cr) contamination which challenges the ecosystem and human health, and chemical stabilization is the most popular one. Limited work focuses on the change of soil microbial community and functions after chemical stabilization...

## Effects of sulfonylurea herbicides chlorsulfuron and sulfosulfuron on enzymatic activities and microbial communities in two agricultural soils

**Authors:** Medo J, Hricakova N, Makova J and more...

**Source:** ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH 27, 33: 41265-41278, 2021, DOI: 10.1007/s11356-020-10063-0

**Abstract:** Sulfonylurea herbicides are widely used for weed control in agriculture, and they are suspected to alter microbial communities and activities in the soil. This study investigates the impact of two sulfonylurea herbicides chlorsulfuron and sulfosulfuron on microbial community and activity in two different soils taken from two sites in west part of the Slovak Republic...

## Responses of soil microbial community to combination pollution of galaxolide and cadmium

**Authors:** Lv Z, Li XG, Wang YJ and more...

**Source:** ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH 2021, DOI: 10.1007/s11356-021-14520-2

**Abstract:** The goal of this work was to assess the effect of combined pollution of galaxolide (HHCB) and cadmium (Cd) on soil microbial community as measured by phospholipid fatty acid (PLFA)...

## Measurement and modeling of hormesis in soil bacteria and fungi under single and combined treatments of Cd and Pb

**Authors:** Fan DW, Sun J, Liu C, and more...

**Source:** SCIENCE OF THE TOTAL ENVIRONMENT 783, 2021, DOI: 10.1016/j.scitotenv.2021.147494

**Abstract:** Heavy metals are considered major environmental pollutants. Soil microorganisms represent a predominant component of soils ecosystems, yet there is little information regarding hormetic responses of soil microorganisms to single and combined exposures to heavy metals...

## Characterizations of heavy metal contamination, microbial community, and resistance genes in a tailing of the largest copper mine in China

**Authors:** Jiang XW, Liu WH, Xu H and more...

**Source:** ENVIRONMENTAL POLLUTION 280, 2021, DOI: 10.1016/j.envpol.2021.116947

**Abstract:** Copper mine tailings are causing great environmental concern nowadays due to their high contents of heavy metals. These hazards may release to air, water, and soil, posing great threat to the living organisms in the surroundings. In the present work, we profiled the heavy metal contents, microbiome and resistome of a mine
tailing in Dexing Copper Mine, which is the largest open-pit copper mine in China...

## Field study on the soil bacterial associations to combined contamination with heavy metals and organic contaminants

**Authors:** Wu YX, Son, QM, Wu JH and more...

**Source:** SCIENCE OF THE TOTAL ENVIRONMENT 778, 2021, DOI: 10.1016/j.scitotenv.2021.146282

**Abstract:** The understanding of soil microbial associations to combined contamination would substantially benefit the restoration of damaged ecosystems, which is currently limited at the field scale. In this study, we investigated the soil bacterial associations to combined contamination with metals (Cd, Cu, Hg, Pb, and Zn), polyaromatic hydrocarbons (PAHs), and polybrominated diphenyl ethers (PBDEs)...

## Effects of long-term discharge of acid mine drainage from abandoned coal mines on soil microorganisms: microbial community structure, interaction patterns, and metabolic functions

**Authors:** Chen Di, Feng QY, Liang HQ

**Source:** ENVIRONMENTAL AND POLLUTION RESEARCH 2021, DOI: 10.1007/s11356-021-14566-2

**Abstract:** More than twenty abandoned coal mines in the Yudong River Basin of Guizhou Province have discharged acid mine drainage (AMD) for a long time. The revelation of microbial community composition, interaction patterns, and metabolic functions can contribute to a better

understanding of such ecosystems, which in its turn can be helpful in the development of strategies aiming at the ecological remediation of AMD pollution. In this study, reference and contaminated soil samples were collected along the AMD flow path for high-throughput sequencing...

## Soil characteristics and microbial community response in rare earth mining areas in southern Jiangxi Province, China

**Authors:** Liang ZT, Zhang WJ, Yang YS and more...

**Source:** ENVIRONMENTAL AND POLLUTION RESEARCH 2021, DOI: 10.1007/s11356-021-14337-z

**Abstract:** The microbial community and functional flora in rare earth mining areas are correlated, but the characteristics and metabolic pathways of pollutant in such mining areas are still poorly known. The heavy metals, rare earth elements, and microorganisms present after mining of rare earth mine sites were analyzed...

## Enzyme activities and microbial functional diversity in metal(loid) contaminated soils near to a copper smelter

**Authors:** Aponte H, Mondaca P, Santander C and more...

**Source:** SCIENCE OF THE TOTAL ENVIRONMENT 779, 2021, DOI: 10.1016/j.scitotenv.2021.146423

**Abstract:** The monitoring of soil metal(loid) contamination is of global significance due to deleterious effects that metal(loid)s have on living organisms. Soil biological properties such as enzyme activities (EAs) are good indicators of metal(loid) contamination due to their high sensitivity, fast response, and low-cost. Here, the
effect of metal(loid) contamination on physicochemical properties and microbial functionality in soils sampled from within 10 km of a Cu smelter is investigated...

## Microbial Ecotoxicity of Biochars in Agricultural Soil and Interactions with Linear Alkylbenzene Sulfonates

**Authors:** Nissen R, Khanal G, Elsgaard L

**Source:** AGRONOMY-BASEL 11, 5, 2021, DOI: 10.3390/agronomy11050828

**Abstract:** Large-scale application of biochar on agricultural land offers the prospect of soil improvement and carbon sequestration for climate-change mitigation. However, negative side-effects on the soil microbial ecosystem are poorly understood, notably in relation to the functions of native microbiomes under realistic routes of biochar exposure. Due to divergent properties, different biochars might interact with soil in complex ways. This might result in decreased or increased ecotoxicity from biochar contaminants, such as heavy metals and polycyclic aromatic hydrocarbons (PAHs)...

## Side effects of traditional pesticides on soil microbial respiration in orchards on the Russian Black Sea coast

**Authors:** Karpun NN, Yanushevskaya EB, Mikhailova YV and more...

**Source:** CHEMOSPHERE 275, 2021, DOI: 10.1016/j.chemosphere.2021.130040

**Abstract:** Agricultural use of pesticides has greatly increased worldwide over the last several decades, affecting soil microorganisms. Microbial basal respiration and substrate-induced respiration rates are commonly used to assess the detrimental effects of pesticides on soil quality.

The goal of the present study was (1) to compare the impact of different pesticides on soil microbial respiration under field conditions, and (2) to characterize the recovery time of soil microbial respiration after pesticide application. The following pesticides were used in the present study: chlorpyrifos, phosalone, dimethoate (organophosphorus insecticides), lambda-cyhalothrin (pyrethroid insecticide), and kresoxim-methyl (fungicide)...

## Gut microbial community response to herbicide exposure in a ground beetle

**Authors:** Giglio A, Vommaro ML, Gionechetti F, Pallavicini A

**Source:** JOURNAL OF APPLIED ENTOMOLOGY 2021, DOI: 10.1111/jen.12919

**Abstract:** Gut microbiota plays a key role in physiological processes of insects, including nutritional metabolism, development, immunity and detoxification. Environmental stressors such as herbicides, used to optimize and improve crop yields, may interfere with the mutualistic relationships causing negative consequences for the host health. Dinitroaniline herbicides, for example pendimethalin, are used worldwide in pre-emergence application to control grass and some broadleaf weeds...

## Field study on the soil bacterial associations to combined contamination with heavy metals and organic contaminants

**Authors:** Wu YX, Song QM, Wu JH and more...

**Source:** SCIENCE OF THE TOTAL ENVIRONMENT 778, 2021, DOI: 10.1016/j.scitotenv.2021.146282

**Abstract:** The understanding of soil microbial associations to combined contamination would
substantially benefit the restoration of damaged ecosystems, which is currently limited at the field scale. In this study, we investigated the soil bacterial associations to combined contamination with metals (Cd, Cu, Hg, Pb, and Zn), polyaromatic hydrocarbons (PAHs), and polybrominated diphenyl ethers (PBDEs)...

CuO, ZnO, and gamma-Fe2O3 nanoparticles modified the underground biomass and rhizosphere microbial community of *Salvia miltiorrhiza* (Bge.) after 165-day exposure

Authors: Wei XM, Cao P, Wang G, and more...

Source: ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 217, 2021, DOI: 10.1016/j.ecoenv.2021.112232

**Abstract:** To investigate whether metal oxide nanoparticles exhibit toxicity or positive effects on medicinal plants, CuO, ZnO, and gamma-Fe2O3 nanoparticles (NPs), at concentrations of 100 and 700 mg kg-1, were introduced into the cultivation of *Salvia miltiorrhiza* (Bge.). Metal elemental contents, chemical constituents, biomass and the structure of the rhizosphere microbial community was used to estimate this effect...

Microbial communities of the drinking water with gradient radon concentration are primarily contributed by radon and heavy metal content

Authors: Nayak T, De D, Karmakar P and more...

Source: FRONTIERS IN ENVIRONMENTAL SCIENCE 9, 2021, DOI: 10.3389/fenvs.2021.576400

**Abstract:** Radon and heavy metal (HM) contamination in drinking water and their impact on health have been reported earlier. However, relatively little is known about the microbial community in drinking water with gradients of radon and the drivers of microbial community patterns in such water...

Salt stress-induced changes in microbial community structures and metabolic processes result in increased soil cadmium availability

Authors: Wang, M, Zhao, SW, Wang, LF, and more...

Source: SCIENCE OF THE TOTAL ENVIRONMENT 782, 2021, DOI: 10.1016/j.scitotenv.2021.147125

**Abstract:** Salt stress can cause significant changes to soil microbial community structure, metabolic processes, and the surrounding micro-environments. However, how these processes affect the availability of cadmium (Cd) remains poorly understood...

Soil characteristics and microbial community response in rare earth mining areas in southern Jiangxi Province, China

Authors: Liang ZT, Zhang WJ, Yang YS and more...

Source: ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH2021, DOI: 10.1007/s11356-021-14337-z

**Abstract:** The microbial community and functional flora in rare earth mining areas are correlated, but the characteristics and metabolic pathways of pollutant in such mining areas are still poorly known. The heavy metals, rare earth
elements, and microorganisms present after
mining of rare earth mine sites were analyzed...

Microbial ecotoxicity of
biochars in agricultural soil
and interactions with linear
alkylbenzene sulfonates

Authors: Nissen R, Khanal G, Elsgaard L

Source: AGRONOMY-BASEL 11, 5, 2021, DOI: 10.3390/agronomy11050828

**Abstract:** Large-scale application of biochar on agricultural land offers the prospect of soil improvement and carbon sequestration for climate-change mitigation. However, negative side-effects on the soil microbial ecosystem are poorly understood, notably in relation to the functions of native microbiomes under realistic routes of biochar exposure...

Effects of heavy metals on
bacterial community
structure in the rhizosphere
of *Salsola collina* and bulk soil
in the Jinchuan mining area

Authors: Gao TP, Wan ZD, Liu XX and more...

Source: GEOMICROBIOLOGY JOURNAL, 2021, DOI: 10.1080/01490451.2021.1914784

**Abstract:** It is important to understand the changes in microbial properties of plant rhizospheres to elucidate the interaction between heavy metals and plant communities. In this study, high throughput sequencing method was used to analyze the effects of heavy metals on rhizosphere microbial community structure of the autochthonous plant *Salsola collina* in the Jinchuan mining area...

Study on the regulatory
mechanism of the earthworm
microbial community in vitro
and in vivo under cadmium
stress

Authors: Zhou DX, Liang XY, Wang JH and more...

Source: ENVIRONMENTAL POLLUTION 279, 2021, DOI: 10.1016/j.envpol.2021.116891

**Abstract:** In this paper, cadmium (Cd) stress tests were performed on *Eisenia fetida* in sterile artificial soil, and its regulatory mechanism between microbial communities in vivo and in vitro after Cd stress was explored...

Bacterial response to soil
property changes caused by
wood ash from wildfire in
forest soils around mining
areas: Relevance of bacterial
community composition,
carbon and nitrogen cycling

Authors: Zhang YY, Yan CC, Liu HJ and more...

Source: JOURNAL OF HAZARDOUS MATERIALS 412, 2021, DOI: 10.1016/j.jhazmat.2021.125264

**Abstract:** The different physical-chemical properties of the black ash (200?500 ?C) and white ash (?&#62;510 ?C) generated by wildfire may result in varied impacts on soil biological and abiotic indicators. Many studies have highlighted the environmental impacts of wood ash application due to its complex mixture of beneficial and detrimental compounds. However, few studies have compared the effect of black ash and white ash on soil, especially for the heavy metal polluted soil...
## Soil bacterial community functions and distribution after mining disturbance

**Authors:** Xiao EZ, Ning ZP, Xiao TF and more...

**Source:** SOIL BIOLOGY & BIOCHEMISTRY 157, 2021, DOI: 10.1016/j.soilbio.2021.108232

**Abstract:** Mining disturbances alter soil edaphic factors, modifying soil biogeochemical processes and thus impacting the soil microbiome. The objectives of this study were (1) to identify the dominant edaphic factor influencing the soil bacterial functions after mining disturbance and (2) to investigate how the soil microbiome was distributed, relative to the dominant edaphic factor...

## Compositional and functional responses of bacterial community to titanium dioxide nanoparticles varied with soil heterogeneity and exposure duration

**Authors:** Zhai YJ, Chen LH, Liu G and more...

**Source:** SCIENCE OF THE TOTAL ENVIRONMENT 773, 2021, DOI: 10.1016/j.scitotenv.2020.144895

**Abstract:** Titanium dioxide nanoparticles (TiO2 NPs) are widely used as nano-agrochemicals. In this study we investigated the influence of soil heterogeneity on bacterial communities exposed to TiO2 NPs over time...

## Spatial variation in microbial community in response to As and Pb contamination in paddy soils near a pb-zn mining site

**Authors:** Zou LN, Lu YH, Dai YX and more...

**Source:** FRONTIERS IN ENVIRONMENTAL SCIENCE 9, 2021, DOI: 10.3389/fenvs.2021.630668

**Abstract:** Mining activity is a growing environmental concern as it contributes to heavy metals (HMs) pollution in agricultural soils. Microbial communities play an important role in the biogeochemical cycling of HMs and have the potential to be used as bioindicators. Arsenic (As) and lead (Pb) are the most hazardous HMs and are mainly originated from mining activities. However, spatial variation in microbial community in response to As and Pb contamination in paddy soils remains overlooked...

## Diversity patterns and drivers of soil microbial communities in urban and suburban park soils of Shanghai, China

**Authors:** Zhang WW, Han JG, Wu HB and more...

**Source:** PEERJ 9, 2021, DOI: 10.7717/peerj.11231

**Abstract:** Background: The rapid expansion of urbanization leads to significant losses of soil ecological functions. Microbes directly participate in key soil processes and play crucial roles in maintaining soil functions. However, we still have a limited understanding of underlying mechanisms shaping microbial communities and the interactions among microbial taxa in park soils...

## High-throughput sequencing clarifies the spatial structures of microbial communities in cadmium-polluted rice soils

**Authors:** Song L, Pan ZZ, Dai Y and more...

**Source:** ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH 2021, DOI: 10.1007/s11356-021-13993-5
**Abstract:** Soil microbial communities are affected by environmental factors. Contamination with heavy metals such as cadmium (Cd) can decrease soil microbial species richness and substantially alter soil microbial species composition. Investigations of the microbial communities in Cd-contaminated soils are necessary to obtain data for soil bioremediation efforts. However, depth-associated variations in microbial community composition and structure in Cd-contaminated paddy soils are not well understood...

## Study of pollutant accumulation characteristics and microbial community impact at three bioretention facilities

**Authors:** Li YJ, Fu H, Zhang JY and more...

**Source:** ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH 2021, DOI: 10.1007/s11356-021-13801-0

**Abstract:** In this paper, three bioretention facilities (BT, RG1-A, and RG1-B) were selected for on-site testing and experimental analysis. Of which, BT is a roadside bioretention tank with layered filler, while RG1-A and RG1-B are rain gardens with conventional filler (Bioretention soil media, BSM) and modified filler (BSM+10% Water treatment residuals, WTR), respectively. The effect of pollutant accumulation on the soil microbial community structure in the facilities, and the risk of heavy metal contamination over several years of bioretention facility operation were studied...

## Microbiological indicators of heavy metals and carbon-containing preparations applied to agrosoddy-podzolic

## soils differing in humus content

**Authors:** Terekhova VA, Prudnikova EV, Kulachkova SA and more...

**Source:** EURASIAN SOIL SCIENCE 54, 3: 448-458, 2021, DOI: 10.1134/S1064229321030157

**Abstract:** The response of the microbial community (microbial biomass carbon (C-mic), basal respiration (BR), and functional diversity (FD)) of agrosoddy-podzolic soil (Albic Glossic Retisols (Loamic, Aric Cutanic, Ochric)) to pollution by heavy metals (HMs: Cu 660, Zn 1100, Pb 650 mg/kg) and carbon-containing preparations (5% of biochar and 0.25% of lignohumate) was studied in model experiment (30 days)...

## Land use in urban areas impacts the composition of soil bacterial communities involved in nitrogen cycling. A case study from Lefkosia (Nicosia) Cyprus

**Authors:** Stephanou C, Omirou M, Philippot L and more...

**Source:** SCIENTIFIC REPORTS 11 1, 2021, DOI: 10.1038/s41598-021-87623-y

**Abstract:** The different types of land-use and soil lithology in urban and peri-urban areas of modern cities compose a complex mosaic of soil ecosystems. It is largely unknown how these differences result in changes in bacterial community composition and structure as well as in functional guilds involved in N cycling. To investigate the bacterial composition and the proportion of denitrifiers in agricultural, forested, schoolyard and industrial areas...
Spatial distribution characteristics of the microbial community and multi-phase distribution of toxic metals in the geochemical gradients caused by acid mine drainage, South China

**Authors:** Pan Y, Ye H, Li XF and more...

**Source:** SCIENCE OF THE TOTAL ENVIRONMENT 774, 2021, DOI: 10.1016/j.scitotenv.2021.145660

**Abstract:** Acid mine drainage (AMD) poses a potential environmental risk due to the low pH combined with high concentrations of toxic metals and sulfate. The Hengshi River in China is continually contaminated by the AMD from upstream mining areas and this river was selected as a study site to investigate the spatial distribution of Cu, Cd, Pb, Zn and the microbial communities in the paddy soil in vicinity of the river...

Insights on the effects of ZnO nanoparticle exposure on soil heterotrophic respiration as revealed by soil microbial communities and activities

**Authors:** Jiang H, Yang BS, Wang H and more

**Source:** JOURNAL OF SOILS AND SEDIMENTS 2021, DOI: 10.1007/s11368-021-02947-6

**Abstract:** Purpose The information on the effects of ZnONPs on soil biological processes and the resulting changes of ecosystem function is limited in wheat straw return agricultural soil. This study investigated the effects of ZnONPs on soil heterotrophic respiration and biochemical properties in wheat straw return farmland soil...

The effects of vehicular emissions on the activity and diversity of the roadside soil microbial community

**Authors:** De Silva S, Ball AS, Shahsavari E and more...

**Source:** ENVIRONMENTAL POLLUTION 277, 2021, DOI: 10.1016/j.envpol.2021.116744

**Abstract:** Motor vehicles emit a variety of pollutants including metals, petroleum hydrocarbons and polycyclic aromatic hydrocarbons (PAHs). The relationships between metals, petroleum hydrocarbons and PAHs, soil respiration and microbial diversity (fungi and bacteria) were studied using control (n = 3) and roadside soils (n = 27) with different exposure periods to vehicle emissions (2e-63 years)...

Synergistic effects of antimony and arsenic contaminations on bacterial, archaeal and fungal communities in the rhizosphere of *Miscanthus sinensis*: Insights for nitrification and carbon mineralization

**Authors:** Yu H, Zheng XF, Weng WL and more

**Source:** JOURNAL OF HAZARDOUS MATERIALS 411, 202, DOI: 10.1016/j.jhazmat.2021.125094

**Abstract:** The impacts of metal(loids) on soil microbial communities are research focuses to understand nutrient cycling in heavy metal-contaminated environments. However, how antimony (Sb) and arsenic (As) contaminations synergistically affect microbially-driven ecological processes in the rhizosphere of plants is poorly understood. Here we examined the
synergistic effects of Sb and As contaminations on bacterial, archaeal and fungal communities in the rhizosphere...

## Impact of pesticide/fertilizer mixtures on the rhizosphere microbial community of field-grown sugarcane

**Authors:** Huang WJ, Lu YL, Chen LJ and more...

**Source:** 3 BIOTECH 11, 5, 2021, DOI: 10.1007/s13205-021-02770-3

**Abstract:** The rhizosphere microbial community is important for plant health and is shaped by numerous environmental factors. This study aimed to unravel the effects of a pesticide/fertilizer mixture on the soil rhizosphere microbiome of field-grown sugarcane. A field trial on sugarcane was conducted...

## Uranium(VI) bioassociation by different fungi - a comparative study into molecular processes

**Authors:** Wollenberg A, Kretzschmar J, Drobot B and more...

**Source:** JOURNAL OF HAZARDOUS MATERIALS 411, 2021, DOI: 10.1016/j.jhazmat.2021.125068

**Abstract:** After the Chernobyl and Fukushima incidents it has become clear that fungi can take up and accumulate large quantities of radionuclides and heavy metals, but the underlying processes are not well understood yet. For this study, the molecular interactions of uranium(VI) with the white-rot fungi, Schizophyllum commune and Pleurotus ostreatus, and the soil-living fungus, Leucoagaricus naucinus, were investigated...

## Ecotoxicity of as-synthesised copper nanoparticles on soil bacteria

**Authors:** Sharma P, Goyal D, Chudasama B

**Source:** IET NANOBIOTECHNOLOGY 15, 2: 236-245, 2021, DOI: 10.1049/nbt2.12039

**Abstract:** Release of metallic nanoparticles in soil poses a serious threat to the ecosystem as they can affect the soil properties and impose toxicity on soil microbes that are involved in the biogeochemical cycling. In this work, in vitro ecotoxicity of as-synthesised copper nanoparticles (CuNPs) on *Bacillus subtilis* (MTCC No. 441) and *Pseudomonas fluorescens* (MTCC No. 1749)...

## Metagenomic insights into taxonomic diversity and metabolic potential of bacterial communities associated with tannery waste-contaminated soils

**Authors:** Lukhele T, Ogola HJO, Selvarajan R and more...

**Source:** INTERNATIONAL JOURNAL OF ENVIRONMENTAL SCIENCE AND TECHNOLOGY; 2021, DOI: 10.1007/s13762-021-03298-y

**Abstract:** Long-term exposure to anthropogenic stressors can lead to perturbations in microbiomes, either selecting for novel and biotechnologically important biotypes, or reductions in the microbial diversity and disequilibrium of ecosystem services. Consequently, analysis of variations in microbial structure and function in stressful environments remains a critical issue in microbial ecology ...
# Impacts of Redox Conditions on Arsenic and Antimony Transformation in Paddy Soil: Kinetics and Functional Bacteria

**Authors:** Xia BQ, Yang Y, Wu YD and more...

**Source:** BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY, 2021, DOI: 10.1007/s00128-021-03242-3

**Abstract:** Arsenic (As) and antimony (Sb) are known carcinogens and are present as contaminants in paddy soils. However, the complicated dynamics of the mobility of these metalloids have not been well understood due to changing redox conditions in paddy soils...

# Changes in soil and rat gut microbial diversity after long-term exposure to the chiral fungicide epoxiconazole

**Authors:** Kaziem AE, He ZZ, Li LS and more...

**Source:** CHEMOSPHERE 272, 2021, DOI: 10.1016/j.chemosphere.2021.129618

**Abstract:** In previous articles, it was found that epoxiconazole enantiomers can persist for a long time in the environment, causing severe environmental damage. Herein, we investigated alterations in the soil microbial community and rat gut microbiota after six weeks of treatment with rac-epoxiconazole or one of its enantiomers...

# Microbial Features Indicating the Recovery of Soil Ecosystem Strongly Affected by Mining and Ore Processing

**Authors:** Feketeova Z, Hrabovsky A, Simkovic I

**Source:** INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH 18, 6, 2021, DOI: 10.3390/ijerph18063240

**Abstract:** Tailings-derived soils formed from waste materials produced during mineral processing often exhibit extremes of pH, low content of organic matter and limited nutrient availability. The success of site revitalization depends mostly on the ability to maintain natural soil functions. We analyzed technogenic sediments from four selected localities in Slovakia defined as environmental burdens...

ERA / PUBLICATIONS

SCIENTIFIQUES /

MICROBIOLOGIE ET

CONTAMINANTS /

Antibiotiques et

antibiorésistances

# Distribution of the microbial community and antibiotic resistance genes in farmland surrounding gold tailings: A metagenomics approach

**Authors:** Qiao LK, Liu XX, Zhang S and more...

**Source:** SCIENCE OF THE TOTAL ENVIRONMENT 779, 2021, DOI: 10.1016/j.scitotenv.2021.146502

**Abstract:** Metal mining has caused the accumulation of waste mine tailing dumps from abandoned mines. The pollution of farmlands surrounding metal tailings by heavy metals has been a long-recognized problem. However, the distribution of antibiotic resistance genes (ARGs) in tailings and the main factors influencing this distribution have rarely been reported. In this study, a metagenomics approach was used to
investigate the microbial community and ARGs present in farmland surrounding gold tailings in northern China...

## Chicken Manure and Mushroom Residues Affect Soil Bacterial Community Structure but Not the Bacterial Resistome When Applied at the Same Rate of Nitrogen for 3 Years

**Authors:** Peng S, Wang YM, Chen RR, Lin XG

**Source:** FRONTIERS IN MICROBIOLOGY 12, 2021, DOI: 10.3389/fmicb.2021.618693

**Abstract:** Animal manure is a reservoir of antibiotic resistance genes (ARGs), and direct application of the manure will lead to spread of ARGs in farmland. Here, we explored the impacts of chicken manure and heat-treated chicken manure on the patterns of soil resistome after 3 years' application, with mushroom residues set as the plant-derived organic manure treatment...

## Habitat determines the relationships among bacteria, resistance genes and mobile genetic elements in the soil-plant system

**Authors:** Huang RL, Ding JX, Guo YW and more...

**Source:** EUROPEAN JOURNAL OF SOIL SCIENCE 2021, DOI: 10.1111/ejss.13132

**Abstract:** The soil antibiotic resistome is considered to be primarily determined by bacterial community composition. However, the antibiotic resistance of plant microbiota and its association with the soil microbiome in soil-plant systems remain largely unknown. Here, we studied the connections between bacteria and

resistance genes (RGs) (mainly antibiotic resistance genes, ARGs) and mobile genetic elements (MGEs) in different cropping systems...

## Bacterial community assembly and antibiotic resistance genes in the lettuce-soil system upon antibiotic exposure

**Authors:** Shen YK, Ryser ELT, Li H, Zhang W

**Source:** SCIENCE OF THE TOTAL ENVIRONMENT 778, 2021, DOI: 10.1016/j.scitotenv.2021.146255

**Abstract:** Bacteria and antibiotic resistance genes (ARGs) in vegetables may influence human gut microbiome and ulti-mately human health. However, little is known about how vegetable microbiomes and ARGs respond to exposure of anthropogenic antibiotics from crop irrigation water. This study investigated bacterial community assembly and ARG profiles in lettuce (Lactuca sativa) shoots and roots, rhizosphere soil, and bulk soil irrigated with antibiotics-containing water, using 16S rRNA amplicon sequencing and high throughput real-time qPCR, respec-tively...

## Distinct resistomes and microbial communities of soils, wastewater treatment plants and households suggest development of antibiotic resistances due to distinct environmental conditions in each environment

**Authors:** Schages L, Wichern F, Geisen S and more...

**Source:** ANTIBIOTICS-BASEL 10, 5, 2021, DOI: 10.3390/antibiotics10050514
**Abstract:** The use of antibiotics in humans and animals results in a release of excess antibiotic residues into the environment through wastewaters and insufficient removal in wastewater treatment plants (WWTP), leading to increasing numbers of bacteria enriched in antibiotic resistance genes (ARG). However, the potential transfer of ARG and their host bacteria between different environments remains largely unexplored...

## Polystyrene microplastics alleviate the effects of sulfamethazine on soil microbial communities at different CO2 concentrations

**Authors:** Xu ML, Du WC, Ai FX and more...

**Source:** JOURNAL OF HAZARDOUS MATERIALS 413, 2021, DOI: 10.1016/j.jhazmat.2021.125286

**Abstract:** Microplastics were reported to adsorb antibiotics and may modify their effects on soil systems. But there has been little research investigating how microplastics may affect the toxicities of antibiotics to microbes under future climate conditions. Here, we used a free-air CO2 enrichment system to investigate the responses of soil microbes to sulfamethazine (SMZ, 1 mg kg(-1)) in the presence of polystyrene microplastics (PS, 5 mg kg(-1)) at different CO2 concentrations...

## Soil Enzymatic Activities and Microbial Community Structure in Soils Polluted with Tetracycline Antibiotics

**Authors:** Santas-Miguel V, Diaz-Ravina M, Martin A and more...

**Source:** AGRONOMY-BASEL 11, 5, 2021, DOI: 10.3390/agronomy11050906

**Abstract:** A laboratory experiment was performed to examine the medium-term influence of three tetracycline antibiotics (chlortetracycline, CTC; tetracycline, TC and oxytetracycline, OTC) at different concentrations in four agricultural soils with similar pH and different soil organic content. After a 42-days incubation period, three different soil enzymes (beta-glucosidase, urease, and phosphomonoesterase) were estimated, as well as the phospholipid fatty acids (PLFAs)...

## Effects of Sulfamethoxazole on Growth and Antibiotic Resistance of A Natural Microbial Community

**Authors:** Rauseo J, Caracciolo AB, Spataro F and more...

**Source:** WATER 13, 9, 2021, DOI: 10.3390/w13091262

**Abstract:** Diffuse environmental antibiotic and antibiotic resistance gene contamination is increasing human and animal exposure to these emerging compounds with a consequent risk of reduction in antibiotic effectiveness. The present work investigated the effect of the antibiotic sulfamethoxazole (SMX) on growth and antibiotic resistance genes of a microbial community collected from an anaerobic digestion plant fed with cattle manure...

## Tetracycline-resistant bacteria and ribosomal protection protein genes in soils from selected agricultural fields and livestock farms

**Authors:** Nogrado K, Unno T, Hur HG, Lee JH

**Source:** APPLIED BIOLOGICAL CHEMISTRY 64, 1, 2021, DOI: 10.1186/s13765-021-00613-6
**Abstract:** Antibiotic resistance in soil environment has eminently been compared and studied between agricultural and pristine soils, and the role of concentrated animal feeding operations has markedly been recognized as one of the major Source:s of antibiotic resistance. This study described the tetracycline resistance in small-scale farms in pursuit of presenting its possible role and contribution to the persistence of antibiotic resistance in the environment...

## Manure-Based Amendments Influence Surface-Associated Bacteria and Markers of Antibiotic Resistance on Radishes Grown in Soils with Different Textures

**Authors:** Guron GKP, Chen CQ, Du P and more...

**Source:** APPLIED AND ENVIRONMENTAL MICROBIOLOGY 87, 10, 2021, DOI: 10.1128/AEM.02753-20

**Abstract:** A controlled greenhouse study was performed to determine the effect of manure or compost amendments, derived during or in the absence of antibiotic treatment of beef and dairy cattle, on radish taproot-associated microbiota and indicators of antibiotic resistance when grown in different soil textures...

## Effects of sulfamethoxazole on growth and antibiotic resistance of a natural microbial community

**Authors:** Rauseo J, Barra Caracciolo A, Spataro F and more...

**Source:** WATER 13, 9, 2021, DOI: 10.3390/w13091262

**Abstract:** Diffuse environmental antibiotic and antibiotic resistance gene contamination is

increasing human and animal exposure to these emerging compounds with a consequent risk of reduction in antibiotic effectiveness. The present work investigated the effect of the antibiotic sulfamethoxazole (SMX) on growth and antibiotic resistance genes of a microbial community collected from an anaerobic digestion plant fed with cattle manure...

## Application of manure from cattle administered antibiotics has sustained multi-year impacts on soil resistome and microbial community structure

**Authors:** Shawver S, Wepking C, Ishii S and more...

**Source:** SOIL BIOLOGY & BIOCHEMISTRY 157, 2021, DOI: 10.1016/j.soilbio.2021.108252

**Abstract:** In agroecosystems, application of manure from livestock treated with antibiotics has the potential to spread antibiotic compounds, resistant bacteria, and antibiotic resistance genes (ARGs) to soil. Although environmental transmission of antibiotic resistance is a major human health concern, few studies have looked at long-term effects on soil microbial communities from applying manure from livestock administered antibiotics...

## Gain and loss of antibiotic resistant genes in multidrug resistant bacteria: One Health perspective

**Authors:** Kim M, Park J, Kang M, Yang J, Park W

**Source:** JOURNAL OF MICROBIOLOGY, 2021, DOI: 10.1007/s12275-021-1085-9

**Abstract:** The emergence of multidrug resistance (MDR) has become a global health threat due to
the increasing unnecessary use of antibiotics. Multidrug resistant bacteria occur mainly by accumulating resistance genes on mobile genetic elements (MGEs), made possible by horizontal gene transfer (HGT). Humans and animal guts along with natural and engineered environments such as wastewater treatment plants and manured soils have proven to be the major reservoirs and hot-spots of spreading antibiotic resistance genes (ARGs)...

## Antibiotrophy: key function for antibiotic-resistant bacteria to colonize soils-case of sulfamethazine-degrading microbacterium sp. C448

**Authors:** Billet L, Pesce S, Rouard N and more...

**Source:** FRONTIERS IN MICROBIOLOGY 12, 2021,

DOI: 10.3389/fmicb.2021.643087

**Abstract:** Chronic and repeated exposure of environmental bacterial communities to anthropogenic antibiotics have recently driven some antibiotic-resistant bacteria to acquire catabolic functions, enabling them to use antibiotics as nutritive Source:s (antibiotrophy). Antibiotrophy might confer a selective advantage facilitating the implantation and dispersion of antibiotrophs in contaminated environments. A microcosm experiment was conducted to test this hypothesis in an agroecosystem context...

## Can heavy metal pollution induce bacterial resistance to heavy metals and antibiotics in soils from an ancient landmine?

**Authors:** Zhong QM, Cruz-Paredes C, Zhang SR
Rousk J

**Source:** JOURNAL OF HAZARDOUS MATERIALS 411, 2021, DOI: 10.1016/j.jhazmat.2020.124962

**Abstract:** Microbial resistance to antibiotics is a growing challenge to human health. Recent evidence has indicated that antibiotic resistance can be co-selected for by exposure to heavy metals in agricultural soils. It remains unknown if this is a concern in other environments contaminated by metals...

## Potential environmental and human health risks caused by antibiotic-resistant bacteria (arb), antibiotic resistance genes (args) and emerging contaminants (ecs) from municipal solid waste (msw) landfill

**Authors:** Anand U, Reddy B, Singh VK and more...

**Source:** ANTIBIOTICS-BASEL 10, 4, 2021, DOI: 10.3390/antibiotics10040374

**Abstract:** The disposal of municipal solid waste (MSW) directly at landfills or open dump areas, without segregation and treatment, is a significant concern due to its hazardous contents of antibiotic-resistant bacteria (ARB), antibiotic resistance genes (ARGs), and metal resistance genes (MGEs). The released leachate from landfills greatly affects the soil physicochemical, biological, and groundwater properties associated with agricultural activity and human health ...
ERA / PUBLICATIONS

SCIENTIFIQUES /

MICROBIOLOGIE ET

CONTAMINANTS /

Biocontrôle

Role of two plant growth-promoting bacteria in remediating cadmium-contaminated soil combined with miscanthus floridulus (lab.)

Authors: Liu SM, Liu HM, Chen R and more...

Source: PLANTS-BASEL 10, 5, 2021, DOI: 10.3390/plants10050912

**Abstract:** Miscanthus spp. are energy plants and excellent candidates for phytoremediation approaches of metal(loid)s-contaminated soils, especially when combined with plant growth-promoting bacteria ...

Physiological and metagenomic strategies uncover the rhizosphere bacterial microbiome succession underlying three common environmental stresses in cassava

Authors: Zeng HQ, Xu HR, Liu GY and more...

Source: JOURNAL OF HAZARDOUS MATERIALS 411, 2021, DOI: 10.1016/j.jhazmat.2021.125143

**Abstract:** The most common environmental pollutants such as cadmium (Cd), glyphosate and

tetracycline have led to profoundly adverse impacts on plant productivity. However, how tropical crops such as cassava sense these pollutants via roots and how rhizosphere microbiome interacts with the host and pollutants remain largely unknown...

Plant growth-promoting rhizobacteria effect on maize growth and microbial biomass in a chromium-contaminated soil

Authors: Silva RS, Antunes JEL, de Aquino JPA and more...

Source: BRAGANTIA 80, 2021, DOI: 10.1590/1678-4499.20200492

**Abstract:** Chromium contamination in soils affects plant growth and this metal can accumulate in plants tissues. In addition, Cr can affect soil microbial biomass and activity. On the other hand, plant growth-promoting rhizobacteria (PGPR) can protect plants against metals and, at the same time, promote plant growth and could alleviate adverse effects on microbial biomass. This study evaluated five PGPR on maize growth, Cr accumulation and soil microbial biomass in a Cr-contaminated soil...

A pesticide biopurification system: a source of biosurfactant-producing bacteria with environmental biotechnology applications

Authors: Lamilla C, Schalchli H, Briceno G and more...

Source: AGRONOMY-BASEL 11, 4, 2021, DOI: 10.3390/agronomy11040624

**Abstract:** Biosurfactants, a wide group of compounds produced by different
microorganisms, generally have less toxicity and are more biodegradable than synthetic surfactants. Biosurfactant-producing bacteria can be found in contaminated environments, such as soils receiving pesticide applications constantly, or in pesticides treatment systems where microorganisms are adapted to biodegrading pesticides. Five pesticide-tolerant bacteria previously isolated from a pesticide biopurification system were evaluated as biosurfactant-producers...

ERA / PUBLICATIONS

SCIENTIFIQUES /

MICROBIOLOGIE ET

CONTAMINANTS /

Bioremédiation

Recruiting endophytic bacteria of wetland plants to phytoremediate organic pollutants

Authors: Singh T, Awasthi G, Tiwari Y

Source: INTERNATIONAL JOURNAL OF ENVIRONMENTAL SCIENCE AND TECHNOLOGY
2021, DOI: 10.1007/s13762-021-03476-y

**Abstract:** The release of organic compounds in the aquatic environment, resulting from different human activities, is a serious hazard due to the toxic, hydrophobic and persistent nature of these compounds. Removing organic contaminants from aquatic systems is vital and is one of the major topics of research in the arena of environmental science. Pesticides, plasticizers, pharmaceuticals, petroleum hydrocarbons are the organic pollutants which are considered to be the emerging contaminants of concern. Phytoremediation is a process of contaminant removal using plants, to clean up surface waters,

groundwaters, sediments and soils. Phytoremediation of organic contaminants can occur through plant uptake, plant enzymes or exudates, or by the contribution of rhizosphere and endophytic organisms...

Degradation of chlorpyriphos and polyethylene by endosymbiotic bacteria from citrus mealybug

Authors: Ibrahim SS, Gupta RK, War AR and more...

Source: SAUDI JOURNAL OF BIOLOGICAL SCIENCES
28, 6: 3214-3224, 2021, DOI: 10.1016/j.sjbs.2021.03.058

**Abstract:** Chlorpyriphos is one of the major organophosphorus pesticides used widely to control a range of insect pests across several crops. This insecticide is hazardous to the environment and toxic to mammals, thus, it is essential to remove the same from the environment. Similarly, use of polythene is also increasing day by day. Therefore, it is highly important to identify ways to degrade chlorpyriphos and other pesticides from the environment...

The microbial community from the early-plant colonizer (Baccharis linearis) is required for plant establishment on copper mine tailings

Authors: Gazitua MC, Morgante V, Poupin MJ and more...

Source: SCIENTIFIC REPORTS 11, 1, 2021, DOI: 10.1038/s41598-021-89769-1

**Abstract:** Plants must deal with harsh environmental conditions when colonizing
abandoned copper mine tailings. We hypothesized that the presence of a native microbial community can improve the colonization of the pioneer plant, Baccharis linearis, in soils from copper mining tailings. Plant growth and microbial community compositions and dynamics were determined in cultivation pots containing material from two abandoned copper mining tailings (Huana and Tambillos) and compared with pots containing fresh tailings or surrounding agricultural soil...

## Effects of arbuscular mycorrhizal fungi on maize (zea mays l.) Under zinc deficient and toxic field conditions

**Authors:** Saboor A., Ali MA

**Source:** APPLIED ECOLOGY AND ENVIRONMENTAL RESEARCH 19, 3: 2151-2169, 2021, DOI: 10.15666/aeer/1903_21512169

**Abstract:** Arbuscular mycorrhizal fungi (AMF) have potential to cope with nutrient stress environment when the soil is zinc (Zn) deficient or toxic...

## Fungal biosorption of cadmium(II) onto *Fennelia nivea* from aqueous solution: equilibrium, thermodynamics, and kinetics

**Authors:** Aracagok YD, Ogun E, Torun M and more...

**Source:** DESALINATION AND WATER TREATMENT 22: 386-393, 2021, DOI: 10.5004/dwt.2021.27091

**Abstract:** Cadmium(II) is an important ecotoxic pollutant and may cause health problems if accumulated in the human body. This study focused on the removal of cadmium(II) from

aqueous solutions using newly isolated fungus dry mass as the adsorber...

## Strategies in microbial degradation enhancement of chlorpyrifos - a review based on the primary approaches in soil bioremediation

**Authors:** Varghese EM, Aswani P, Jisha MS

**Source:** BIOCATALYSIS AND BIOTRANSFORMATION 2021, DOI: 10.1080/10242422.2021.1939693

**Abstract:** The worldwide demand for potential agrochemicals is escalating day-by-day. Unsystematic and widespread pesticide use has resulted in constant expansion of pesticide industry making them the leaders of the agrochemical industry. The consumption of organophosphate pesticides is rapidly rising leading to its persistence in the environment thereby resulting in environmental contamination. Chlorpyrifos (O,O-diethyl O-(3,5,6-trichloro-2-pyridyl) phosphorothioate) is the most broadly used wide-spectrum chlorinated organophosphate pesticide...

## Motor oil wastewater treatment in a packed bed bioreactor using immobilized native microbial consortium

**Authors:** Houbron E, Cruz-Carmona E, Ponciano-Rosas A and more...

**Source:** REVISTA MEXICANA DE INGENIERIA QUIMICA 20(2): 761-773, 2021, DOI: 10.24275/rmiq/IA2271

**Abstract:** The global increase in population has led to a higher demand for motor oil from a raising number of automobiles. The inappropriate disposal of waste motor oil generates water contamination, therefore, removing motor oil from contaminated water can prevent damage to
the environment and human health. In this study, a new native microbial consortium from contaminated soil was tested and exposed to different concentrations of motor oil...

## Insight into Cr(VI) biosorption onto algal-bacterial granular sludge: Cr(VI) bioreduction and its intracellular accumulation in addition to the effects of environmental factors

**Authors:** Yang XJ, Zhao ZW, Zhang GH and more...

**Source:** JOURNAL OF HAZARDOUS MATERIALS 414, 2021, DOI: 10.1016/j.jhazmat.2021.125479

**Abstract:** Hexavalent chromium (Cr(VI)) is one of the typical heavy metals that pose a great threat to the environment. As a novel biotechnology, algal-bacterial aerobic granular sludge (AGS) possesses the merits of both bacterial AGS and algae. This study firstly evaluated Cr(VI) removal via biosorption by algal-bacterial AGS under different operation conditions and then some environmental factors...

## Improved immobilization of soil cadmium by regulating soil characteristics and microbial community through reductive soil disinfestation

**Authors:** Li X, Li XF, Li YY and more...

**Source:** SCIENCE OF THE TOTAL ENVIRONMENT 778, 2021, DOI: 10.1016/j.scitotenv.2021.146222

**Abstract:** Cadmium (Cd) contamination arising from industrialization has attracted increasing attention in recent years. Reductive soil disinfestation (RSD) as an effective agricultural practice has been widely applied for soil

sterilization. However, there is little research regarding RSD affecting Cd immobilization...

## Effects of magnetic biochar-microbe composite on Cd remediation and microbial responses in paddy soil

**Authors:** Wang L, Chen HR, Wu JZ and more...

**Source:** JOURNAL OF HAZARDOUS MATERIALS 414, 2021, DOI: 10.1016/j.jhazmat.2021.125494

**Abstract:** There is growing global interest in the bioremediation of cadmium (Cd) using combinations of biochar and microorganisms. However, the interactions among biochar, introduced and indigenous microorganisms remain unclear. Accordingly, a 90 day microcosm experiment was conducted to investigate this by adding Bacillus sp. K1 strain inoculated rice straw biochar (SBB) and magnetic straw biochar (MBB) into a Cd contaminated paddy soil from Hunan, China...

## Biodegradation pathway of polycyclic aromatic hydrocarbons by ligninolytic fungus podoscypha elegans strain ftg4 and phytotoxicity evaluation of their metabolites

**Authors:** Agrawal N, Barapatre A, Shahi MP, Shahi SK

**Source:** ENVIRONMENTAL PROCESSES-AN INTERNATIONAL JOURNAL 2021, DOI: 10.1007/s40710-021-00525-z

**Abstract:** Polycyclic aromatic hydrocarbons (PAHs) are a type of persistent toxic organic pollutants with potential carcinogenicity and mutagenicity, therefore, dangerous for the safety
of human and animal health. The present study investigated the biodegradation of phenanthrene (PHE) and pyrene (PYR) in in-vitro and in-vivo conditions, by the white rot fungus Podoscypha elegans strain FTG4, isolated from an old rotten wood log through the lignin enrichment method...

## Microbial and abiotic factors of flooded soil that affect redox biodegradation of lindane

**Authors:** Yuan J, Jue ST, Ma B and more...

**Source:** SCIENCE OF THE TOTAL ENVIRONMENT 780, 2021, DOI: 10.1016/j.scitotenv.2021.146606

**Abstract:** Pollution induces pressure to soil microorganism; and conversely, the degradation of pollutants is reported largely regulated by the soil microbiome assembly in situ. However, the specific-dependent core taxa of degraders were barely confirmed, which is not conducive to improving the soil remediation strategy. Taking pollution of a typical organochlorine pesticide (OCP), lindane, as an example, we explored the microbial community assembly in flooded soils and simultaneously quantified the corresponding dynamics of typical soil redox processes...

## Phytostabilization of acidic mine tailings with biochar, biosolids, lime, and locally-Sourced microbial inoculum: Do amendment mixtures influence plant growth, tailing chemistry, and microbial composition?

**Authors:** Trippe KM, Manning VA, Reardon C and more...

**Source:** APPLIED SOIL ECOLOGY 165, 2021, DOI: 10.1016/j.apsoil.2021.103962

**Abstract:** Abandoned mine lands present persistent environmental challenges to ecosystems and economies; reclamation is an important step for overcoming these challenges. Phytostabilization is an elegant and cost-effective reclamation strategy, however, establishing plants on severely degraded soils is problematic, often requiring soil amendments. We evaluated whether amendment mixtures composed of lime, biochar, biosolids, and locallySource:d microbial inoculum (LSM) could alleviate the constraints that hinder phytostabilization success...

## Current insights into the microbial degradation for butachlor: strains, metabolic pathways, and molecular mechanisms

**Authors:** Lin ZQ, Pang SM, Zhou Z and more...

**Source:** APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2021, DOI: 10.1007/s00253-021-11346-3

**Abstract:** The herbicide butachlor has been used in huge quantities worldwide, affecting various environmental systems. Butachlor residues have been detected in soil, water, and organisms, and have been shown to be toxic to these non-target organisms...

## The multi metal-resistant bacterium Cupriavidus
metallidurans CH34 affects
growth and metal
mobilization in *Arabidopsis
thaliana* plants exposed to
copper

**Authors:** Clavero-Leon C, Ruiz D, Cillero J and more...

**Source:** PEERJ 9, 2021, DOI: 10.7717/peerj.11373

**Abstract:** Copper (Cu) is important for plant growth, but high concentrations can lead to detrimental effects such as primary root length inhibition, vegetative tissue chlorosis, and even plant death. The interaction between plant-soil microbiota and roots can potentially affect metal mobility and availability, and, therefore, overall plant metal concentration...

Role of plant growth
promoting bacteria in driving
speciation gradients across
soil-rhizosphere-plant
inter interfaces in zinc-
contaminated soils

**Authors:** Adele NC, Ngwenya BT, Heal KV, Mosselmans JFW

**Source:** ENVIRONMENTAL POLLUTION 279, 2021, DOI: 10.1016/j.envpol.2021.116909

**Abstract:** Inoculation of soil or seeds with plant growth promoting bacteria ameliorates metal toxicity to plants by changing metal speciation in plant tissues but the exact location of these changes remains unknown. Knowing where the changes occur is a critical first step to establish whether metal speciation changes are driven by microbial metabolism or by plant responses...

Bacterial isolates from
Argentine Pampas and their
ability to degrade glyphosate

**Authors:** Masotti F, Garavaglia BS, Piazza A and more...

**Source:** SCIENCE OF THE TOTAL ENVIRONMENT 774, 2021, DOI: 10.1016/j.scitotenv.2021.145761

**Abstract:** Glyphosate is a synthetic phosphonate compound characterized by a carbon-phosphorus bond. Glyphosate based herbicides (GBH) are widely distributed in most of the economically productive lands in which crop production is mainly based on glyphosate-resistant genetically modified plants. Naturally, glyphosate is remediated by soil microorganisms, which accelerate its degradation...

Carboxylic acid reduction and
sulfate-reducing bacteria
stabilization combined
remediation of Cr (VI)-
contaminated soil

**Authors:** Gu WZ, Cui MY, Tian C and more...

**Source:** ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 218, 2021, DOI: 10.1016/j.ecoenv.2021.112263

**Abstract:** For controlling heavy metal pollution, the utilization of carboxylic acids (CAs) combined with sulfate-reducing bacteria (SRB) for continuous and stable remediation of Cr (VI)-contaminated soil was comprehensively investigated...
# Biodegradation Pathway of Polycyclic Aromatic Hydrocarbons by Ligninolytic Fungus *Podoscypha elegans* Strain FTG4 and Phytotoxicity Evaluation of their Metabolites

**Authors:** Agrawal, N, Barapatre, A, Shahi, MP, Shahi, SK

**Source:** ENVIRONMENTAL PROCESSES-AN INTERNATIONAL JOURNAL 2021, DOI: 10.1007/s40710-021-00525-z

**Abstract:** Polycyclic aromatic hydrocarbons (PAHs) are a type of persistent toxic organic pollutants with potential carcinogenicity and mutagenicity, therefore, dangerous for the safety of human and animal health. The present study investigated the biodegradation of phenanthrene (PHE) and pyrene (PYR) in in-vitro and in-vivo conditions, by the white rot fungus *Podoscypha elegans* strain FTG4, isolated from an old rotten wood log through the lignin enrichment method...

# Contribution of Nano-Zero-Valent Iron and Arbuscular Mycorrhizal Fungi to Phytoremediation of Heavy Metal-Contaminated Soil

**Authors:** Cheng P, Zhang SQ, Wang QL and more...

**Source:** NANOMATERIALS 11, 5, 2021, DOI: 10.3390/nano11051264

**Abstract:** Soil pollution with heavy metals has attracted increasing concern, which calls for the development of new remediation strategies. The combination of physical, chemical, and biological techniques can achieve more efficient remediation. However, few studies have focused on whether nanomaterials and beneficial microbes can be jointly used to facilitate

phytoremediation. Therefore, we studied the role of nano-zero-valent iron (nZVI) and arbuscular mycorrhizal (AM) fungi in the phytoremediation of an acidic soil polluted with Cd, Pb and Zn, using sweet sorghum. X-ray diffraction (XRD), energy dispersive X-ray spectroscopy (EDS), and mapping analyses were conducted to explore the mechanisms of metal immobilization by nZVI...

# Microbial and abiotic factors of flooded soil that affect redox biodegradation of lindane

**Authors:** YuanJ, Jue ST, Ma B, Lu ZJ and more...

**Source:** SCIENCE OF THE TOTAL ENVIRONMENT 780, 2021, DOI: 10.1016/j.scitotenv.2021.146606

**Abstract:** Pollution induces pressure to soil microorganism; and conversely, the degradation of pollutants is reported largely regulated by the soil microbiome assembly in situ. However, the specific-dependent core taxa of degraders were barely confirmed, which is not conducive to improving the soil remediation strategy. Taking pollution of a typical organochlorine pesticide (OCP), lindane, as an example, we explored the microbial community assembly in flooded soils and simultaneously quantified the corresponding dynamics of typical soil redox processes...

# Fungal biostimulant-driven phytoextraction of heavy metals from tannery solid waste contaminated soils

**Authors:** Nazir A, Shafiq M, Bareen FE

**Source:** INTERNATIONAL JOURNAL OF PHYTOREMEDIATION 2021, DOI: 10.1080/15226514.2021.1924115

**Abstract:** Two of the multiple limitations of phytoextraction efficiency (PE %) of TSW polluted soils are: (i) low growth of plant performance, (ii)
poor bioavailability of excessive essential and heavy metals (ascribed as Category-I and II metals respectively) The current study reports biostimulant role of allochthonous Trichoderma harzianum (F1) and autochthonous Trichoderma pseudokoningii (F2) in growth of Tagetes patula L. and uptake of Category-I & II metals from TSW-soil (0, 5 et 10%)...

## An overview of neonicotinoids: biotransformation and biodegradation by microbiological processes

**Authors:** Anjos CS, Lima RN, Porto ALM

**Source:** ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH2021, DOI: 10.1007/s11356-021-13531-3

**Abstract:** ...This review discusses the most recent microbial biodegradation and bioremediation processes for neonicotinoids, which employ isolated microorganisms (bacteria and fungi), consortiums of microorganisms, and different types of soils, biobeds, and biomixtures.

## Synergistic Effects of Zinc Oxide Nanoparticles and Bacteria Reduce Heavy Metals Toxicity in Rice (Oryza sativa L.) Plant

**Authors:** Akhtar N, Khan S, Rehman SU and more...

**Source:** TOXICS 9, 5, 2021, DOI: 10.3390/toxics9050113

**Abstract:** Heavy metals (HMs) are toxic elements which contaminate the water bodies in developing countries because of their excessive discharge from industrial zones. Rice (Oryza sativa L) crops are submerged for a longer period of time in

water, so irrigation with HMs polluted water possesses toxic effects on plant growth. This study was initiated to observe the synergistic effect of bacteria (Bacillus cereus and Lysinibacillus macroides) and zinc oxide nanoparticles (ZnO NPs) (5, 10, 15, 20 and 25 mg/L) on the rice that were grown in HMs contaminated water...

## Effects of Soil Properties on Phytoextraction of Cd and the Associated Soil Bacterial Communities across Four Soil Types

**Authors:** Hou F, Du JJ

**Source:** SOIL & SEDIMENT CONTAMINATION, 2021, DOI: 10.1080/15320383.2021.1926420

**Abstract:** Soils are one of the repositories for heavy metal. Phytoextraction with hyperaccumulator *Tagetes patula* (T.patula) has great promise for the cadmium (Cd) removal from contaminated soils. However, there is lack of information about the effects of soil types with different properties on the phytoextraction efficiency and the associated bacterial communities. A pot experiment...

## Ureolytic bacteria from electronic waste area, their biological robustness against potentially toxic elements and underlying mechanisms

**Authors:** Li WL, Fishman A, Achal V

**Source:** JOURNAL OF ENVIRONMENTAL MANAGEMENT 289, 2021, DOI: 10.1016/j.jenvman.2021.112517

**Abstract:** Ureolytic bacteria can be a promising mediator used for the immobilization of potentially toxic elements via microbially-induced carbonate precipitation (MICP) process from
biodegradable ions to carbonate form. Electronic waste (E-waste) environment is very complex compared to general metal contaminated soil, however, MICP has not been studied under such an environment. In this study, three bacterial strains were successfully isolated from an E-waste area...

## Metagenomics reveals functional profiling of microbial communities in OCP contaminated sites with rapeseed oil and tartaric acid biostimulation

**Authors:** Zhang ZY, Wan JZ, Liu L and more...

**Source:** JOURNAL OF ENVIRONMENTAL MANAGEMENT 289, 2021, DOI: 10.1016/j.jenvman.2021.112515

**Abstract:** Organochlorine pesticides (OCPs) contaminated sites pose great threats to both human health and environmental safety. Targeted bioremediation in these regions largely depends on microbial diversity and activity. This study applied metagenomics to characterize the microbial communities and functional groups composition features during independent or simultaneous rapeseed oil and tartaric acid applications, as well as the degradation kinetics of OCPs...

## Metagenomic analysis of microbial community and its role in bioelectrokinetic remediation of tannery contaminated soil

**Authors:** Prakash AA, Rajasekar A, Sarankumar RK and more...

**Source:** JOURNAL OF HAZARDOUS MATERIALS 412, 2021, DOI: 10.1016/j.jhazmat.2021.125133

**Abstract:** Tanneries create a serious threat to the environment by generating a significant amount of toxic metal-containing solid waste. This study deals with the application of bio-electrokinetic remediation (Bio-EK) of tannery effluent contaminated soil (TECS). Metagenomes representing the TECS sample were sequenced using the Illumina HiSeq platform...

## Effects of different dissolved organic matter on microbial communities and arsenic mobilization in aquifers

**Authors:** Wang YH, Zhang GL, Wang HL and more...

**Source:** JOURNAL OF HAZARDOUS MATERIALS 411, 2021, DOI: 10.1016/j.jhazmat.2021.125146

**Abstract:** Dissolved organic matter (DOM) play key roles in the biotransformation of arsenic in groundwater systems. However, the effects of different types of DOM on arsenic biogeochemistry remain poorly understood. In this study, four typical DOM compounds (acetate, lactate, AQS and humic acid) were amended to high As aquifer sediments to investigate their effects on arsenic/iron biotransformation and microbial community response...

## Heavy Metal-Resistant Filamentous Fungi as Potential Mercury Bioremediators

**Authors:** Vacar CL, Covaci E, Chakraborty S and more...

**Source:** JOURNAL OF FUNGI 7, 5, 2021, DOI: 10.3390/jof7050386

**Abstract:** Filamentous fungi native to heavy metals (HMs) contaminated sites have great potential for bioremediation, yet are still often underexploited. This research aimed to assess the HMs resistance and Hg remediation capacity of
fungi isolated from the rhizosphere of plants resident on highly Hg-contaminated substrate...

## Microbial bioremediation of heavy metals

**Authors:** Volaric A, Svircev Z, Tamindzija D, Radnovic D

**Source:** HEMIJSKA INDUSTRIJA 75, 2: 103-115, 2021, DOI: 10.2298/HEMIND200915010V

**Abstract:** Heavy metal pollution is one of the most serious environmental problems, due to metal ions persistence, bioavailability, and toxicity. There are many conventional physical and chemical techniques traditionally used for environmental clean-up. Due to several drawbacks regarding these methods, the use of living organisms, or bioremediation, is becoming more prevalent...

## The participation of cyanobacteria in reducing the concentration of fusariotoxins and heavy metal ions in aqueous solutions (model experiments)

**Authors:** Domracheva LI, Skugoreva SG, Fokina AJ and more...

**Source:** SOUTH OF RUSSIA-ECOLOGY DEVELOPMENT 16, 1: 53-60, 2021, DOI: 10.18470/1992-1098-2021-1-53-60

**Abstract:** Aim. The goal was to establish the effect of soil cyanobacteria (CB) Fischerella muscicola, Nostoc paludosum and Nostoc linckia on change in the concentration of heavy metal ions (HM) and fusariotoxins in aqueous media...

## Heavy metals in soils and the remediation potential of bacteria associated with the plant microbiome

**Authors:** Henao SG, Ghneim-Herrera T

**Source:** FRONTIERS IN ENVIRONMENTAL SCIENCE 9, 2021, DOI: 10.3389/fenvs.2021.604216

**Abstract:** High concentrations of non-essential heavy metals/metalloids (arsenic, cadmium, and lead) in soils and irrigation water represent a threat to the environment, food safety, and human and animal health. Microbial bioremediation has emerged as a promising strategy to reduce the concentration of heavy metals in the environment due to the demonstrated ability of microorganisms, especially bacteria, to sequester and transform these compounds...

## Plant growth-promoting rhizobacteria effect on maize growth and microbial biomass in a chromium-contaminated soil

**Authors:** Silva RS, Antunes JEL, de Aquino JPA and more...

**Source:** BRAGANTIA 80, 2021, DOI: 10.1590/1678-4499.20200492

**Abstract:** Chromium contamination in soils affects plant growth and this metal can accumulate in plants tissues. In addition, Cr can affect soil microbial biomass and activity. On the other hand, plant growth-promoting rhizobacteria (PGPR) can protect plants against metals and, at the same time, promote plant growth and could alleviate adverse effects on microbial biomass...
# A review on mechanism of biomineralization using microbial-induced precipitation for immobilizing lead ions

**Authors:** Shan B, Hao RX, Xu H and more...

**Source:** ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH 2021, DOI: 10.1007/s11356-021-14045-8

**Abstract:** Lead (Pb) is a toxic metal originating from natural processes and anthropogenic activities such as coal power plants, mining, waste gas fuel, leather whipping, paint, and battery factories, which has adverse effects on the ecosystem and the health of human beings. Hence, the studies about investigating the remediation of Pb pollution have aroused extensive attention. Microbial remediation has the advantages of lower cost, higher efficiency, and less impact on the environment...

# Microbial community responses to land-use types and its ecological roles in mining area

**Authors:** Xiao EZ, Wang YQ, Xiao TF and more...

**Source:** SCIENCE OF THE TOTAL ENVIRONMENT 775, 2021, DOI: 10.1016/j.scitotenv.2021.145753

**Abstract:** Mining activities result in adverse impacts on soil ecosystems, leading to environmental damage and ecological degradation. Soil microorganisms are considered critical for soil ecosystem restoration. However, questions about how soil microbiomes respond to land-use types and their beneficial roles in soil restoration have received little attention ...

# The physiological response of Ectomycorrhizal fungus *Lepista sordida* to Cd and Cu stress

**Authors:** Yin DC, Qi JY

**Source:** PEERJ 9, 2021, DOI: 10.7717/peerj.11115

**Abstract:** Ectomycorrhizal fungi (ECMF) can develop the resistance of host plants to heavy metal stress. However, little is known about the response of ECMF to heavy metal exposure. In this study, the growth and physiological indices of *Lepista sordida* under Cd and Cu stress were studied...

# Microbial degradation of recalcitrant pesticides: a review

**Authors:** Bose S, Kumar PS, Vo DVN and more...

**Source:** ENVIRONMENTAL CHEMISTRY LETTERS 2021, DOI: 10.1007/s10311-021-01236-5

**Abstract:** Some pesticides such as organochlorines are of critical environmental concern because they are highly persistent due to their stable chemical nature. As a consequence, even after banning, dichlorodiphenyltrichloroethane and endosulfan can be detected at concentrations above permissible limits. Moreover, classical pesticide degradation of these compounds using physiochemical processes is limited. Alternatively, biodegradation using microorganisms isolated in contaminated sites appears promising...
# Effect of poultry manure compost and arbuscular mycorrhizal fungi on Cu immobilization and soil microbial communities in a Cu-contaminated soil using the metallophyte *Oenothera picensis*

**Authors:** Meier S, Moore F, Khan N and more...

**Source:** JOURNAL OF SOIL SCIENCE AND PLANT NUTRITION 2021, DOI: 10.1007/s42729-021-00493-1

**Abstract:** Soil metal contamination has emerged as a global environmental issue due to toxicity to living organisms. Traditional remediation techniques applied to metal-contaminated soils are intrusive and expensive, by which more environmental-friendly methods, such as phytoremediation, are necessary to remediate metal-contaminated soils. This study aimed to evaluate the combined effect of poultry manure compost (PMC) and indigenous arbuscular mycorrhizal (IM) fungi inoculation over copper (Cu) immobilization and the effectiveness of these elements promoting the plant growth and the soil microbial communities in a Cu-contaminated soil...

# Multiple heavy metals immobilization based on microbially induced carbonate precipitation by ureolytic bacteria and the precipitation patterns exploration

**Authors:** Qiao SY, Zeng GQ, Wang XT and more...

**Source:** CHEMOSPHERE 274, 2021, DOI: 10.1016/j.chemosphere.2021.129661

**Abstract:** Biomineralization to immobilize the toxic metal has great potential for the bioremediation of multiple heavy metal contamination. In this study, the efficiency of Microbially Carbonate Induced Precipitation (MICP) for several common heavy metals (Cu, Zn, Ni, Cd) in mining areas as well as their precipitation patterns were researched...

# Removal of Iron from Aqueous Solution by Novel Bacteria Isolated from Marine Macro Alga

**Authors:** Mishra A, Christian V, Kouame LM, Saraf M

**Source:** BIOSCIENCE BIOTECHNOLOGY RESEARCH COMMUNICATIONS 13, 1: 194-198, 2021, DOI: 10.21786/bbrc/13.1specialissue/31

**Abstract:** Iron is the most abundant metal element on earth, and iron-bearing minerals are everywhere and that are reactive element of water, soils, and sediments. Iron is a beneficial when it is in permissible limit when excess amount of iron is present it becomes toxic. Current technologies for removal and recovery of toxic elements are the industrial interest and metals usually produce wastes with high concentrations...

# Bio-mercury remediation suitability index: a novel proposal that compiles the pgpr features of bacterial strains and its potential use in phytoremediation

**Authors:** Robas M, Jimenez PA, Gonzalez D, Probanza A
**Source:** INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH 18, 8, 2021, DOI: 10.3390/ijerph18084213

**Abstract:** Soil pollution from heavy metals, especially mercury, is an environmental problem for human health. Biological approaches offer interesting tools, which necessarily involve the selection of organisms capable of transforming the environment via bioremediation. To evaluate the potential use of microorganisms in phytorhizoremediation, bacterial strains were isolated from rhizospheric and bulk soil...

## Effects of microbial spatial distribution on organic biodegradation and immobilization of trace metals in co-contaminated soils

**Authors:** Masum SA, Thomas HR

**Source:** COMPUTERS AND GEOTECHNICS 133, 2021, DOI: 10.1016/j.compgeo.2021.104063

**Abstract:** Predicting the transport and fate of organic and inorganic contaminants in co-contaminated soils is challenging, since various complex, inter-related processes are involved. In the presence of soil microbes that metabolise organic compounds (e.g., phenol), the chemical and the physical properties of the soil may alter and consequently affect the transport of inorganic trace metals e.g., Cu, Cd, Ni, Pb...

## Characterization of soil bacteria with potential to degrade benzoate and antagonistic to fungal and bacterial phytopathogens

**Authors:** Esikov, TZ, Anokhina TO, Abashina TN and more...

**Source:** MICROORGANISMS 9, 4, 2021, DOI: 10.3390/microorganisms9040755

**Abstract:** The intensive development of agriculture leads to the depletion of land and a decrease in crop yields and in plant resistances to diseases. A large number of fertilizers and pesticides are currently used to solve these problems. Chemicals can enter the soil and penetrate into the groundwater and agricultural plants. Therefore, the primary task is to intensify agricultural production without causing additional damage to the environment. This problem can be partially solved using microorganisms with target properties...

## Review on Pesticides Application in Agriculture and its Effect on Environment with Brief on their Microbial Degradation

**Authors:** Chouhan S, Mulani R, Goswami D

**Source:** BIOSCIENCE BIOTECHNOLOGY RESEARCH COMMUNICATIONS 13, 1: 239-250, 2020, DOI: 10.21786/bbrc/13.1specialissue/38

**Abstract:** Pesticides utilization for preventing insect pests, weeds in crops and improving production has led to application of pesticides...

## Frenemies: interactions between rhizospheric bacteria and fungi from metalliferous soils

**Authors:** Rosatto S, Cecchi G, Roccotiello E and more...

**Source:** LIFE-BASEL 11, 4, 273, 2021, DOI: 10.3390/life11040273

**Abstract:** Is it possible to improve the efficiency of bioremediation technologies? The use of mixed cultures of bacteria and fungi inoculated at the
rhizosphere level could promote the growth of the associated hyperaccumulating plant species and increase the absorption of metals in polluted soils, broadening new horizons on bioremediation purposes...

## Green remediation of toxic metals contaminated mining soil using bacterial consortium and Brassica juncea

**Authors:** Jeyasundar PGSA, Ali A, Azeem M and more...

**Source:** ENVIRONMENTAL POLLUTION 277, 2012, DOI: 10.1016/j.envpol.2021.116789

**Abstract:** Microorganism-assisted phytoremediation is being developed as an efficient green approach for management of toxic metals contaminated soils and mitigating the potential human health risk. The capability of plant growth promoting Actinobacteria (Streptomyces pactum Act12 - ACT) and Firmicutes (Bacillus subtilis and Bacillus licheniformis - BC) in mono-and co-applications (consortium) to improve soil properties and enhance phytoextraction of Cd, Cu, Pb, and Zn by Brassica juncea (L.) Czern. was studied...

## The effects of vermicompost and shell powder addition on Cd bioavailability, enzyme activity and bacterial community in Cd-contaminated soil: A field study

**Authors:** Wang F, Zhang WW, Miao LJ and more...

**Source:** ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 215, 2021, DOI: 10.1016/j.ecoenv.2021.112163

**Abstract:** Cadmium (Cd) contamination has become serious in soil and in situ stabilization technology has been widely used for heavy metal remediation. A field study was conducted to determine the effect of amendments with the doses of 3 kg/m2, including single vermicompost (A1), a 95% vermicompost mixed with 5% shell powder composite (A2) and a 95% vermicompost mixed with 5% modified shell powder composite (A3), on the Cd bioavailability, enzyme activity and bacterial community in soil...

## Improvement of alfalfa resistance against Cd stress through rhizobia and arbuscular mycorrhiza fungi co-inoculation in Cd-contaminated soil

**Authors:** Wang X, Fang LC, Beiyuan JZ and more...

**Source:** ENVIRONMENTAL POLLUTION 277, 2021, DOI: 10.1016/j.envpol.2021.116758

**Abstract:** Rhizobia and arbuscular mycorrhiza fungi (AMF) are important symbiotic microbes that are advantageous to plants growing in metal-contaminated soil. However, it remains unclear how inoculated microbes affect rhizosphere microbial communities or whether subsequent changes in rhizosphere microbiomes contribute to improving plant resistance under metal stress...

## Biodegradation of 4-chloro-2-methylphenoxyacetic acid by endophytic fungus Phomopsis sp. In liquid medium and soil

**Authors:** Wang Y, Feng G, Li H., Du L, Zeng D
**Source:** APPLIED ECOLOGY AND ENVIRONMENTAL RESEARCH 19, 2: 1295-1307, 2021, DOI: 10.15666/aeer/1902_12951307

**Abstract:** An endophytic fungus strain, newly isolated from Psidium guajava (common guava), could effectively degrade 4-chloro-2-methylphenoxyacetic acid (MCPA). According to the analysis of its phenotypic feature and ITS rDNA gene sequence, the isolated strain was identified as Phomopsis sp. and named E41. The influencing factors including medium pH, temperature, and initial concentration of MCPA affecting the degradation of MCPA by this strain were optimized in this study...

Insight into metal immobilization and microbial community structure in soil from a steel disposal dump phytostabilized with composted, pyrolyzed or gasified wastes

**Authors:** Radziemska M, Gusiatin ZM, Cydzik-Kwiatkowska A and more...

**Source:** CHEMOSPHERE 272, 2021, DOI: 10.1016/j.chemosphere.2021.129576

**Abstract:** The soil system is a key component of the environment that can serve as a sink of pollutants. Using processed waste for aided phytostabilization of metals (HMs) in contaminated soils is an attractive phytoremediation technique that integrates waste utilization and recycling. In this study, we evaluated the effect of biologically and thermally processed wastes...

ERA / PUBLICATIONS
SCIENTIFIQUES /
PESTICIDES/CLIMAT
et VdT/ENCHY

Measurement of
multixenobiotic resistance
activity in enchytraeids as a
tool in soil ecotoxicology

**Authors:** Kovacevic M, Hackenberger DK, Loncaric Z, Hackenberger BK

**Source:** CHEMOSPHERE 279: 130549, 2021, DOI: 10.1016/j.chemosphere.2021.130549

**Abstract:** The multixenobiotic resistance (MXR) mechanism is the first defense line against xenobiotics. Enchytraeids, a model organism in soil ecotoxicology, are often exposed to various xenobiotics, some of which may influence MXR activity. Since MXR activity has not been studied in these organisms, the aim of this paper was to establish a methodology for the implementation of the dye assay in enchytraeids...

Ecotoxicological effects of the
pyrethroid insecticide
tefluthrin to the earthworm
Eisenia fetida: a chiral view

**Authors:** Wen Y, Zhou LL, Li D, La Q, Shi HY, Wang MH

**Source:** ENVIRONMENTAL RESEARCH 190: 109991, 2021, DOI: 10.1016/j.envres.2020.109991

**Abstract:** Tefluthrin was the first pyrethroid developed for soil treatment. There was no report about the toxicity to terrestrial invertebrates at the enantiomer level. The main objective of the present study was to investigate the enantiomer-
specific acute toxicity to the earthworm *Eisenia fetida* and potential mechanism via multilevel response. (...)

## Biomarker responses and metabolism in *Lumbricus terrestris* exposed to drugs of environmental concern, an in vivo and in vitro approach

**Authors:** Sole M, Montemurro N, Perez S

**Source:** CHEMOSPHERE 277: 130283, 2021, DOI: 10.1016/j.chemosphere.2021.130283

**Abstract:** The earthworm *Lumbricus terrestris* is an anecic species living in natural soils but it is also a sentinel in pollution monitoring. Specimens of L. terrestris were exposed for 48 h though the filter paper contact test at 1 mg/mL of the chemicals: Lamotrigine (LMG), Cocaine (COC), Fipronil (FIP) and the pesticide bis-4-nitrophenyl phosphate (BNPP). (...)

## Pesticides and soil invertebrates: a hazard assessment

**Authors:** Gunstone T, Cornelisse T, Klein K, Dubey A, Donley N

**Source:** FRONTIERS IN ENVIRONMENTAL SCIENCE 9: 643847, 2021, DOI: 10.3389/fenvs.2021.643847

**Abstract:** (...) Many terrestrial invertebrates have declined in recent decades. Habitat loss and agrichemical pollution due to agricultural intensification have been identified as major driving factors. Here, we review nearly 400 studies on the effects of pesticides on non-target invertebrates that have egg, larval, or immature development in the soil. (...)

## Soil biodiversity: state-of-the-art and possible implementation in chemical risk assessment

**Authors:** van Gestel CAM, Mommer L, Montanarella L, Pieper S et al...

**Source:** INTEGRATED ENVIRONMENTAL ASSESSMENT AND MANAGEMENT 17: 541-551, 2021, DOI: 10.1002/ieam.4371

**Abstract:** (...) The central aim of the SESSS14 was to provide information on how to include soil biodiversity and soil functions as protection goal options in the risk assessment and quantification of the effects of chemicals and other stressors (including their respective regulations). (...)

## Effects of glyphosate-based herbicides and their active ingredients on earthworms, water infiltration and glyphosate leaching are influenced by soil properties

**Authors:** Zaller JG, Weber M, Maderthaner M, Gruber E, Takacs E, Mortl M, Klatyik S et al.

**Source:** ENVIRONMENTAL SCIENCES EUROPE 33: 51, 2021, DOI: 10.1186/s12302-021-00492-0

**Abstract:** (...) In a greenhouse experiment, we established a weed population with common amaranth (*Amaranthus retroflexus*) to examine the effects of three GBHs (Roundup LB Plus, Roundup PowerFlex, Touchdown Quattro) and their corresponding Als (salts of glyphosate isopropylammonium, potassium, diammonium) on the activity and physiological biomarkers (glutathione S-transferase, GST; acetylcholine esterase, AChE) of an ecologically relevant earthworm species (*Lumbricus terrestris*). (...)
# The pursuit of Alachlor herbicide toxicity on *Eisenia fetida* and its biochemical responses

**Authors:** Gangadhar D, Babu PV, Pamanji R, Srikanth K

**Source:** WATER AIR AND SOIL POLLUTION 232: 149, 2021, DOI: 10.1007/s11270-021-05109-z

**Abstract:** Alachlor is a chloroacetanilide herbicide, which is extensively used in agriculture globally. (...) Earthworms are largely involved in increasing the soil fertility, and excess use of this herbicide may result in toxicity to burrowing organisms like earthworms. In this context, the current intent of the proposed work is to assess the toxicity of alachlor on *Eisenia fetida*. (...)

ERA / PUBLICATIONS

SCIENTIFIQUES / PLASTIQUES

# Attached and planktonic bacterial communities on bio-based plastic granules and micro-debris in seawater and freshwater

**Authors:** Nguyen NHA, El-Temsah YS, Cambier S et al.

**Source:** SCIENCE OF THE TOTAL ENVIRONMENT 785:147413, 2021, DOI: 10.1016/j.scitotenv.2021.147413

**Abstract:** Bio-based plastics, produced from renewable biomass Sources, may contribute to lowering greenhouse gases and the demand for fossil reSource:s. However, their environmental fate is not well understood. Here, we compared the impacts of industrially produced granules (G

and micro-debris (MD) from three pristine bio-based plastics: high-density polyethylene (HDPE), polylactic acid (PLA) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) on natural bacterial communities in seawater and freshwater using metagenomics...

# Comparison of the effects of continuous and accumulative exposure to nanoplastics on microalga *Chlorella pyrenoidosa* during chronic toxicity

**Authors:** Yang WF, Gao P, Nie Y et al.

**Source:** SCIENCE OF THE TOTAL ENVIRONMENT 788:147934, 2021, DOI: 10.1016/j.scitotenv.2021.147934

**Abstract:** Most previous studies have focused on the continuous exposure of aquatic organisms to nanoplastics. However, persistent pollutants in natural aquatic surroundings are a threat, and their concentrations are continuously increasing. The discussion and research into the effects of accumulative exposure to these materials are limited. Therefore, this study aimed to compare the effects of continuous and accumulative exposure to polystyrene (PS) nanoplastics (80 nm) on Chlorella pyrenoidosa during chronic toxicity...

# Effect of microplastics on growth and biochemical composition of microalga *Acutodesmus obliquus*

**Authors:** Ansari FA, Ratha SK, Renuka N et al.

**Source:** ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 56:102296, 2021, DOI: 10.1016/j.algal.2021.102296

**Abstract:** Microplastics have been widely detected in different aquatic ecosystems. The
toxicity of microplastics has been extensively evaluated on aquatic animals; however, there is limited information on their influence on microalgal growth and metabolism. This study investigates the effect of different concentrations (0, 5, 10, 15, 25, 100, 125, 200, and 250 mg L-1), of microplastics namely high-density polyethylene, polypropylene, and polyvinyl chloride exposure on growth, photosynthesis, and biochemical composition of *Acutodesmus obliquus...*

## Plastic degradation by extremophilic bacteria

**Authors:** Atanasova N, Stoitsova S, Paunova-Krasteva T, Kambourova M

**Source:** INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 22:5610, 2021, DOI: 10.3390/ijms22115610

**Abstract:** [...] This review represents a first attempt to summarize the extraordinarily limited information on biodegradation of conventional synthetic plastics by thermophilic, alkaliphilic, halophilic, and psychrophilic bacteria in natural environments and laboratory conditions...

## Effects of Plastic Debris on the Biofilm Bacterial Communities in Lake Water

**Authors:** Shen C, Huang LY, Xie, GW et al.

**Source:** WATER 13:1465, 2021, DOI:10.3390/w13111465

**Abstract:** Increasing discharge of plastic debris into aquatic ecosystems and the worsening ecological risks have received growing attention. Once released, plastic debris could serve as a new substrate for microbes in waters. The complex relationship between plastics and biofilms has aroused great interest. To confirm the hypothesis that the presence of plastic in water affects the composition of biofilm in natural state, in situ biofilm culture experiments were conducted in a

lake for 40 days. The diversity of biofilm attached on natural (cobble stones and wood) and plastic substrates (Polyethylene terephthalate and Polymethyl methacrylate) were compared, and the community structure and composition were also analyzed...

## Mechanism of the inhibition and detoxification effects of the interaction between nanoplastics and microalgae *Chlorella pyrenoidosa*

**Authors:** Yang WF, Gao P, Li HX et al.

**Source:** SCIENCE OF THE TOTAL ENVIRONMENT 783:146919, 2021, DOI: 10.1016/j.scitotenv.2021.146919

**Abstract:** Most previous studies have focused on the toxicity of microplastics on aquatic organisms. However, research on nanoplastics is still limited and poses significant threat to aquatic organisms than microplastics. Therefore, this study investigated the effects of nanoplastics (80 nm) on the microalgae *Chlorella pyrenoidosa*. One unanticipated finding was that inhibition and detoxification effects existed in the interaction between nanoplastics and *C. pyrenoidosa...*

## Microplastics contamination in the protected agricultural soils and its effects on bacterial community diversity

**Authors:** Fei YF, Huang SY, Wang JQ and more...

**Source:** CHINESE SCIENCE BULLETIN-CHINESE 66(13): 1592-1601, 2021, DOI: 10.1360/TB-2020-0685

**Abstract:** Mircroplastics (MPs) are defined as plastic particles or fibers of size &#60;5 mm and have been of concern in terrestrial ecosystems for several years. Protected agricultural soils have been assumed to be potentially contaminated
with MPs because of the use of large amounts of plastic films and annual compost inputs. However, there is little published information on MP contamination of protected agricultural soils and its ecological effects on soil biota remain unknown...

## Soil type driven change in microbial community affects poly(butylene adipate-co-terephthalate) degradation potential

**Authors:** Han YJ, Teng Y, Wang X and more...

**Source:** ENVIRONMENTAL SCIENCE & TECHNOLOGY 55(8): 4648-4657, 2021, DOI: 10.1021/acs.est.0c04850

**Abstract:** Biodegradable mulch films have been developed as a suitable alternative to conventional nondegradable polyethylene films. However, the key factors controlling the degradation speed of biodegradable mulch films in soils remain unclear. Here, we linked changes in the soil microbiome with the degradation rate of a promising biodegradable material poly (butylene adipate-co-terephthalate) (PBAT) in four soil types...

## Aged microplastics decrease the bioavailability of coexisting heavy metals to microalga *Chlorella vulgaris*

**Authors:** Wang ZZ, Fu DD, Gao L et al.

**Source:** ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 217:112199, 2021, DOI: 10.1016/j.ecoenv.2021.112199

**Abstract:** Environmental aging of ubiquitous microplastics (MP) occurs through the action of biotic and abiotic factors, and aged MP exhibit different physicochemical properties and environmental behavior from virgin MP. This study

aimed to investigate the aged micro-sized polystyrene (mPS) and polyvinyl chloride (mPVC), and the heavy metals copper (Cu) and cadmium (Cd), and examine the effects of their combined toxicities on microalga *Chlorella vulgaris...*

## Effect thresholds for the earthworm *Eisenia fetida*: Toxicity comparison between conventional and biodegradable microplastics

**Authors:** Ding WL, Li Z, Qi RM, Jones DL, Liu QY et al.

**Source:** SCIENCE OF THE TOTAL ENVIRONMENT 781: 146884, 2021, DOI: 10.1016/j.scitotenv.2021.146884

**Abstract:** Biodegradable plastics have been developed to eliminate the progressive accumulation and ever-growing threat posed by conventional fossil fuel-derived plastics. The impact of these bioplastics, particularly in an agricultural context (e.g. biopolymer mulch films), however, remains poorly understood. In this study, we compared the biotoxicity of biodegradable (polylactic acid, PLA; polypropylene carbonate, PPC) and non-degradable (polyethylene, PE) microplastics using a series of standardized bioassays using the earthworm *Eisenia fetida*. The responses studied included: avoidance behavior, mortality, biomass, and reproduction responses. We incubated earthworms in artificial soils amended with different concentrations of microplastic (0, 0.125, 1.25, 12.5, 125, 250, and 500 g kg(-1)) under laboratory conditions...

## The rise of artificial soil carbon inputs: Reviewing
## microplastic pollution effects in the soil environment

**Authors:** Mbachu O, Jenkins G, Kaparaju P, Pratt C

**Source:** CIENCE OF THE TOTAL ENVIRONMENT 780: 146569, 2021, DOI: 10.1016/j.scitotenv.2021.146569

**Abstract:** The surge in the use of plastic materials, its poor handling and disposal have led to an increase in microplastic pollution in terrestrial environments. Microplastic pollution in soils is of concern due to potential influences on soil properties which play a critical role in plant growth and soil fertility. Moreover, the soil environment is a key nexus linking the atmosphere, hydrosphere, biosphere and lithosphere, and thus represents a crucial conduit for pollutant migration from the anthroposphere. In this review we evaluate the effects of microplastics in the soil environment with a specific focus on physical properties and biological function in the rhizosphere...

## Microplastics contamination in the protected agricultural soils and its effects on bacterial community diversity

**Authors:** Fei YF, Huang SY, Wang JQ, Luo YM, Zhang HB

**Source:** CHINESE SCIENCE BULLETIN-CHINESE 66(13): 1592-1601, 2021, DOI: 10.1360/TB-2020-0685

**Abstract:** Mircroplastics (MPs) are defined as plastic particles or fibers of size < 5 mm and have been of concern in terrestrial ecosystems for several years. Protected agricultural soils have been assumed to be potentially contaminated with MPs because of the use of large amounts of plastic films and annual compost inputs. However, there is little published information on MP contamination of protected agricultural soils and its ecological effects on soil biota remain

unknown. Here, a total of 54 surface (0-10 cm depth) soils were sampled from intensively managed protected agricultural soils around Hangzhou Bay. east China. MPs were extracted from the soil samples using continuous air flotation followed by density separation. Stereomicroscopy and micro-Fourier transform infrared spectroscopy (mu-FTIR) were used for identification after the extracted MPs were pre-sorted based on their shapes. In addition, a 29-d soil incubation experiment was conducted to observe the changes in microbial community diversity

## Soil microbial community and network changes after long-term use of plastic mulch and nitrogen fertilization on semiarid farmland

**Authors:** Liu JL, Li SQ, Yue SC, Tian JQ, Chen H et al.

**Source:** GEODERMA 396: 115086, 2021, DOI: 10.1016/j.geoderma.2021.115086

**Abstract:** Soil microbes are crucial for improving soil quality and productivity. Plastic film mulch (FM), in conjunction with fertilization, has significantly improved crop yields over vast areas of dryland production. However, how these practices affect soil microbial communities, especially as regards co-occurrence patterns within microbial taxa, is unclear. The objective of this study was to determine the effects of 10 years of FM and four nitrogen (N) fertilization rates [0 (NO), 100 (N100), 250 (N250), and 400 (N400) kg N ha⁻¹ (-1)] on soil bacterial and fungal diversity, community structure, composition, and the co-occurrence network in a rainfed maize (Zea mays L.) field on the Loess Plateau of China....

## Effects of biofilm colonization on the sinking of
## microplastics in three
freshwater environments

**Authors:** Miao LZ, Gao YX, Adyel TM et al.

**Source:** JOURNAL OF HAZARDOUS MATERIALS 413:125370, 2021, DOI:10.1016/j.jhazmat.2021.125370

**Abstract:** Microplastics (MPs) have frequently been detected in freshwater environments, and there is growing concern about their ecological effects, especially the influence of the "plastisphere" on the freshwater ecosystems. The colonization of microbes on MPs would significantly alter their transport behavior, i.e., buoyancy, in fresh water. In this research, we studied the effects of biofilm colonization on the sinking and floating of three MPs, i.e., polyethylene terephthalate, polypropylene, and polyvinyl chloride, after 44 days of incubation in three freshwater systems (the Niushoushan River, the Qinhuai River, and East Lake) in China...

## Bacterial and fungal
assemblages and functions
associated with biofilms differ
between diverse types of
plastic debris in a freshwater
system

**Authors:** Wang LF, Tong JX, Li Y et al.

**Source:** ENVIRONMENTAL RESEARCH 196:110371, 2021, DOI: 10.1016/j.envres.2020.110371

**Abstract:** Once in aquatic ecosystems, plastics can be easily colonized by diverse microbes, and these microbial communities on plastics -the 'plastisphere'- often differ from the communities in the surrounding water and other substrates. However, our knowledge of plastic-associated bacterial and fungal communities on diverse plastics in freshwater is poor, especially for fungal communities. Furthermore, intraspecies interactions among bacterial and fungal communities colonized on diverse plastics are

poorly known. Here, we characterized the taxonomic composition and diversity of bacteria and fungi on three types of plastics in a lab-scale incubator with freshwater from an urban river...

## Genomic and proteomic
profiles of biofilms on
microplastics are decoupled
from artificial surface
properties

**Authors:** Oberbeckmann S, Bartosik D, Huang SX et al.

**Source:** ENVIRONMENTAL MICROBIOLOGY Early Access, 2021, DOI: 10.1111/1462-2920.15531

**Abstract:** Microplastics in marine ecosystems are colonized by diverse prokaryotic and eukaryotic communities. How these communities and their functional profiles are shaped by the artificial surfaces remains broadly unknown. In order to close this knowledge gap, we set up an in situ experiment with pellets of the polyolefin polymer polyethylene, the aromatic hydrocarbon polymer polystyrene, and wooden beads along a coastal to estuarine gradient in the Baltic Sea, Germany. We used an integrated metagenomics/metaproteomics approach to evaluate the genomic potential as well as protein expression levels of aquatic plastic biofilms...

## Combined effects of
microplastics and biochar on
## the removal of polycyclic aromatic hydrocarbons and phthalate esters and its potential microbial ecological mechanism

**Authors:** Ren XW, Tang JC, Wang L, Sun HW

**Source:** FRONTIERS IN MICROBIOLOGY 12, 2021, DOI: 10.3389/fmicb.2021.647766

**Abstract:** Microplastics (MPs) have been attracting wide attention. Biochar (BC) application could improve the soil quality in the contaminated soil. Currently, most studies focused on the effect of MPs or BC on the soil properties and microbial community, while they neglected the combined effects...

## Effect of plastic film mulching on the distribution of plastic residues in agricultural fields

**Authors:** Kumar MV, Sheela AM

**Source:** CHEMOSPHERE 273:28590, 2021, DOI: 10.1016/j.chemosphere.2020.128590

**Abstract:** In the present study, plastic residues were identified in the following four regions of Tamil Nadu, India: Sulur, Nagondapalli, Royakottah, and Krishnagiri. Among these regions, the percent distribution of plastic residues was found to be higher in Sulur of Coimbatore District where plastic film mulching has been practiced for 10 years for cultivating tomato (Lycopersicum esculentus L.) crop. At different depths, namely 0-10, 11-20, and 21-30 cm, the distribution of plastic residues at Sulur was 37.97%, 35.07%, and 36.99%, respectively, compared with other regions. The distribution of plastic residues was found to decrease with increasing depth...

## The microplastisphere: Biodegradable microplastics addition alters soil microbial

## community structure and function

**Authors:** Zhou J, Gui H, Banfield CC and more...

**Source:** SOIL BIOLOGY & BIOCHEMISTRY 156, 2021, DOI: 10.1016/j.soilbio.2021.108211

**Abstract:** Plastics accumulating in the environment, especially microplastics (defined as particles &#60;5 mm), can lead to a range of problems and potential loss of ecosystem services. Polyhydroxyalkanoates (PHAs) are biodegradable plastics used in mulch films, and in packaging material to minimize plastic waste and to reduce soil pollution. Little is known, however, about the effect of microbioplastics on soil-plant interactions, especially soil microbial community structure and functioning in agroecosystems. For the first time, we combined zymography (to localize enzyme activity hotspots) with substrate-induced growth respiration to investigate the effect of PHAs addition on soil microbial community structure, growth, and exoenzyme kinetics in the microplastisphere (i.e. interface between soil and microplastic particles) compared to the rhizosphere and bulk soil...

## Trading tree growth for soil degradation: Effects at 10 years of black plastic mulch on fine roots, earthworms, organic matter and nitrate in a multi-species riparian buffer

**Authors:** Jones A, Fortier J, Gagnon D, Truax B

**Source:** TREES FORESTS AND PEOPLE 2: 100032, 2020, DOI: 10.1016/j.tfp.2020.100032

**Abstract:** This study assessed the effects of two vegetation management treatments (black polyethylene mulch and a control treatment) on tree growth, fine root biomass of trees and of competing vegetation, earthworms, and
physiochemi-cal soil properties in a 10 year-old agricultural riparian buffer planted in southern Québec (Canada) with five trees species: Populus ×canadensis Moench (hybrid poplar), Fraxinus pennsylvanica Marsh. (red or green ash), Quercus macrocarpa Michx. (bur oak), Quercus rubra L. (red oak), and Pinus strobus L. (eastern white pine). Vegetation management treatments had a significant effect on tree growth, fine root biomass, earthworms and on many soil properties. Overall, the use of plastic mulch increased tree growth in all species (not to the same extent), but locally decreased soil organic matter, total soil carbon, total soil nitrogen, earthworm biomass and abundance, competing vegetation fine root biomass and the biomass of the total pool of fine roots...

# Diversity and structure of microbial biofilms on microplastics in riverine waters of the Pearl River Delta, China

**Authors:** Yang GQ, Gong MT, Mai L et al.

**Source:** CHEMOSPHERE 272:129870, 2021, DOI: 10.1016/j.chemosphere.2021.129870

**Abstract:** Riverine runoff is a significant transport pathway for microplastics (MPs) discharged from land-based Source:s to marine environments where MPs accumulate. Knowledge of riverine MP-associated biofilms will improve the understanding of the fate and potential effects of MPs in marine environments. This study aimed to characterize the microbial biofilms colonizing MPs in the riverine water of the Pearl River Delta, China, and identify the seasonal, geographical and environmental influences on MP-associated communities...

# DROIT ET POLITIQUE DE L'ENVIRONNEMENT

## Protection des abeilles et autres insectes pollinisateurs et préservation des services de pollinisation lors de l'utilisation des produits phytopharmaceutiques - Consultations publiques

**Ministère de la Transition Ecologique**

Ce projet d'arrêté interministériel vise à renforcer les dispositions réglementaires de protection des pollinisateurs vis-à-vis de l'exposition aux produits phytopharmaceutiques. Il constitue une action du projet de plan national en faveur des insectes pollinisateurs et de la pollinisation 2021-2026.

La consultation, effectuée en application de l'article L. 123-19-1 du code de l'environnement, aura lieu jusqu'au 20 juillet 2021. [...]

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## Identification des alternatives au glyphosate pour les usages non agricoles

**Ministère Agriculture 28/05/21**

Une mission interministérielle, composée du CGAER et du CGEDD, a été chargée d'identifier les alternatives non chimiques au glyphosate pour les usages non agricoles et non forestiers (zones non agricoles), en particulier pour la SNCF et les sites industriels, dans le cadre du plan de sortie de cette substance active.

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# EPA releases updated occupational pesticide handler and post-application exposure calculators

EPA May 26, 2021

EPA has updated the [Occupational Pesticide Handler Exposure Calculator](https://www.inrao.fr/en/epa/occupational-pesticide Handler-Exposure-Calculateur) and [Occupational Pesticide Post-application Exposure Calculator](https://www.inrao.fr/en/epa/post-application-exposure-calculateur) with the latest available data and Source:s to provide the agency with more efficiency in completing risk assessments and ensuring transparency to the public and affected stakeholders.

EPA uses these tools to carry out risk assessments to make informed decisions when approving new pesticides, new uses of registered pesticides, and during regular reviews of existing pesticides. The updated exposure values are more reflective of actual exposures to occupational pesticide handlers and post-application scenarios, which reduces uncertainty in decisions and serves as the basis for labeling decisions. [...]

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## Substance active terbuthylazine : conditions d'approbation

RÈGLEMENT D'EXÉCUTION (UE) 2021/824 DE LA COMMISSION du 21 mai 2021 modifiant les règlements d'exécution (UE) n°540/2011 et (UE) n°820/2011 en ce qui concerne les conditions d'approbation de la substance active terbuthylazine

Numéro officiel : UE/2021/824
Date de signature : 21/05/2021
Liens juridiques : Modification Règlement d'exécution UE/540/2011 25/05/2011

Modification Règlement d'exécution UE/820/2011 16/08/2011

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## Approbation renouvelée de la substance active cyazofamid

RÈGLEMENT D'EXÉCUTION (UE) 2021/843 DE LA COMMISSION du 26 mai 2021 renouvelant l'approbation de la substance active cyazofamid conformément au règlement (CE) no 1107/2009 du Parlement européen et du Conseil concernant la mise sur le marché des produits phytopharmaceutiques, et modifiant l'annexe du règlement d'exécution (UE) no 540/2011 de la Commission

Numéro officiel : UE/2021/843
Date de signature : 26/05/2021
Liens juridiques : Modification Règlement d'exécution UE/540/2011 25/05/2011

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## Approbation renouvelée de la substance active Streptomyces souche K61

RÈGLEMENT D'EXÉCUTION (UE) 2021/853 DE LA COMMISSION du 27 mai 2021 renouvelant l'approbation de la substance active Streptomyces souche K61, conformément au règlement (CE) no 1107/2009 du Parlement européen et du Conseil concernant la mise sur le marché des produits phytopharmaceutiques, et modifiant l'annexe du règlement d'exécution (UE) no 540/2011 de la Commission

Numéro officiel : UE/2021/853
Date de signature : 27/05/2021
Liens juridiques : Modification Règlement d'exécution UE/540/2011 25/05/2011

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