# Wild pea Pisum fulvum and Pisum elatius chromosome segment substitution lines in cultivated P. sativum genetic background

Lenka Svabova, Gregoire Aubert, P. Smykal

► To cite this version:

Lenka Svabova, Gregoire Aubert, P. Smykal. Wild pea Pisum fulvum and Pisum elatius chromosome segment substitution lines in cultivated P. sativum genetic background. 2. International Legume Society Conference - Legumes for a Sustainable World, Legume Society., Oct 2016, Lisbonne, Portugal. 358 p. hal-01605501

HAL Id: hal-01605501

https://hal.science/hal-01605501v1

Submitted on 3 Jun 2020

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# Legumes for a Sustainable World

## ILS2 | Second International Legume Society Conference

11th - 14th OCT 2016

TRÓIA RESORT | PORTUGAL

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UNIVERSIDADE
NOVA
DE LISBOA

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Wild pea *Pisum fulvum* and *Pisum elatius* chromosome
segment substitution lines in cultivated *P. sativum* genetic
background

Lenka S.¹, Gregoire A.², Smykal P.¹

1. Dep.of Botany, Palack University in Olomouc, lechtitel 27, 783 71 Olomouc, Czech Republic.

2 - INRA, Dijon, France

Plant evolution under domestication has altered numerous traits,
introducing domestication bottleneck resulting in high degree of
relatedness, leading to narrower genetic base of cultivated germplasm,
prone to pests and diseases. The study of genetic diversity showed that
although wide diversity is captured among cultivated pea, wild material
provides yet broader diversity (Smýkal et al. 2013, 2015). The
chromosome segment substitution lines (CSSL) containing genomic
segments of wild pea (Pisum fulvum WL2140 or Pisum elatius L100) in
the cultivated pea (P. sativum subsp. sativum cv. Terno or cv. Cameor)
genetic background were developed. These lines have been molecularly
analyzed using microsatellite and gene-specific markers at 2 to 82 cM
spacing at early generations (BC2-3F2-4). There were 5 to 14 segments
per line, with mean of 9.6. Higher density genotyping of 50 selected
BC3F6 P. fulvum/Terno CSSL lines using pea 13.2k Pea SNP (Tayeh et al.
2015) and further 100 lines using DARTseq approach is in progress.
Establishment of such permanent introgression library will allow
phenotypic characterization of unlimited number of target traits, which,
coupled together with higher density markers, will provide means for
QTL and gene identification and subsequent incorporation in desired
genotypes. Field testing of agronomical performance of 50 lines of P.
fulvum/Terno CSSL lines is under way.

This work received funding from the European Community's Seventh
Framework Programme (FP).