# Chemical Treatment of Black Tiger nut Tubers (Cyperusesculentus) Variety to Obtain a Bio-based Product

Djomdi Djomdi, Richard Ejoh, Guillaume Pierre, Cedric Delattre, Philippe Michaud, Robert Ndjouenkeu

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## To cite this version:

Djomdi Djomdi, Richard Ejoh, Guillaume Pierre, Cedric Delattre, Philippe Michaud, et al.. Chemical Treatment of Black Tiger nut Tubers (Cyperusesculentus) Variety to Obtain a Bio-based Product. Journal of Nutrition and Food Processing, 2021, 4, pp.01 - 08. 10.31579/2637-8914/069. hal-04935835

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# Chemical Treatment of Black Tiger nut Tubers
*(Cyperusesculentus) Variety to Obtain a Bio-based Product*

DJOMDI<sup>1,2*</sup>, Richard EJOH<sup>1</sup>, Guillaume PIERRE<sup>3</sup>, Cedric DELATTRE<sup>3,4</sup>, Philippe MICHAUD<sup>3</sup> and Robert NDJOUENKEU<sup>1</sup>

**\*Corresponding Author:** DJOMDI, Department of Food Science and Nutrition, University of Ngaoundéré, P.O. Box 455 Ngaoundéré, Cameroon.

**Received date:** August 17, 2021; **Accepted date:** September 13, 2021; **Published date:** September 20, 2021

**Citation:** DJOMDI, Richard EJOH, Guillaume PIERRE, Cedric DELATTRE, Philippe MICHAUD et al. (2021). Chemical Treatment of Black Tiger nut Tubers (Cyperusesculentus) Variety to Obtain a Bio-based Product. *J. Nutrition and Food Processing*, 4(6); DOI:10.31579/2637-8914/069

**Copyright:** © 2020 DJOMDI, This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

## Abstract

The purpose of this study is to improve microbiological qualities of black tiger nut tubers. Thereby, black variety of tiger nut tubers (*Cyperus esculentus*) were differently processed in ascorbic acid and alkaline (Ca(OH)₂ and Kanwa (alkaline calcium carbonate locally used to soften vegetable)) solutions at different concentrations and temperatures. The effect of these treatments on the water content (swelling), soluble loss, dehulling efficiency and microbial load was investigated. Water content or swelling of the tiger nut soaked in acid and alkaline solutions can be predicted by Peleg model like with brown variety of C. esculentus tubers. This swelling lead to enhance dehulling efficiency of tuber but caused soluble solute loss overall at the highest temperature of soaking in vitamin C solution. Like soaking in water, the constant of Peleg (k₁) decrease with the increase of the temperature of soaking, whereas the constant of capacity of Peleg (k₂) is approximately 0.018% for all the conditions. Dehulling efficiency also increased with increasing temperatures (65% at 20°C to 70% at 60°C) for tiger nut tubers soaked in water (0 g/L), dehulling efficiency appeared most strongly affected by concentrations and temperatures of soaking solutions. Like dehulling efficiency, ascorbic solutions has given the best results in term of reduction of microbial load compared to the other soaking solutions. This reduction is considerable at the higher temperatures, reaching a maximum of 4.8 log reduction. Soaking and dehulling treatments gave to black tiger nut tuber effective decontamination in term of microbial load and obtained good microbiological quality for final process.

**Key words:** *cyperusesculentus*; soaking; husking; microorganism; tiger nuts; black tubers; bacteria and moulds; vegetable milk

## Introduction

Black variety of tiger nut tubers (*Cyperusesculentus*) are used for production of vegetable milk or few data are available in literature concerning pretreatment of tubers, extraction and biochemical composition of milk [1a-4]. In Spain, brown variety is used in the production of a milky drink called “horchata de chufa”. High level of microorganisms such as bacteria and moulds (more than 109cfu/ml) have been detected in it; limiting its shelf life (less than 24 hours) [5-7]. These micro-organisms originate from the rugous surface of the tuber where

they are encrusted in the anfractuosities of the collenchyma sleeve [6-8]. The rough structure of the tuber surface does not allow the easy elimination of micro-organisms, even when using sodium hypochlorite [7]. Tubers soaked in water during several hours undergo a turgescence which results in an easy elimination of the tuber external skin [9, 10]. This turgescence is not observed with hypochlorite solution after 48 hours, suggesting the processing time is not sufficient. So, the research of a cleansing process of tiger nut tubers remains a major concern for a better use of them in the production of vegetable milk. It proves to be important to test treatments having given convincing results with other raw

<footnote n="1">¹Department of Food Science and Nutrition, University of Ngaoundéré, P.O. Box 455 Ngaoundéré, Cameroon.</footnote>

<footnote n="2">²National Advanced School of Engineering, University of Maroua P.O. Box 46 Maroua, Cameroon.</footnote>

<footnote n="3">³Clermont Auvergne University, CNRS, SIGMA Clermont, Institut Pascal, F-63000 Clermont-Ferrand, France.</footnote>

<footnote n="4">⁴University Institute of France (IUF), 1 Rue Descartes, 75005 Paris, France.</footnote>
materials requiring a dehulling process [10]. The swelling of tiger nut tuber consecutive to soaking in acid or alkaline solutions is likely to facilitate its dehulling. These treatment are consequently better elimination of its initial microbial load according to previous studies focusing on the dehulling of black bean and maize grains by treatment with alkaline solutions [11, 12] and Solutions of calcium hydroxide, Kanwa (alkaline calcium carbonate locally used to soften vegetables and ascorbic were used for this purpose like describe by Djomdi et al. [1a].

Thus, this work presents strategies (soaking and dehulling) to improve microbiological qualities of black tiger nut tubers for better later use.

# Material and Methods

## Preparation of samples

Black variety of tiger nut tubers were bought on the market of Guily in the Far-north Region of Cameroon, which is the most producing zone of these foodstuffs (Figure 1).

<figure><img src="image_3.png" /><figcaption>Figure 1: Tigernut plant and black variety tubers</figcaption></figure>

Tubers were sorted by screening on metallic sieve in order to retain tubers with diameter ≥ 1 cm. The tubers were then washed with tap water to remove sands and other undesirable materials (stones, pebbles, dirt materials, rotten stems and broken tubers), then sun dried [10].

## Soaking experiments

Soaking was undergone according to the methods of Turhan et al. [13], Sayar and Gunasekaran [14] and Djomdi et al. [10] and Djomdi et al. [15b]. The process consists in soaking the tubers in beakers of 250 mL containing 100 mL solution of Ca(OH)₂, kanwa or ascorbic acid at 20, 40 and 60°C. The beakers were placed in a constant temperature water bath for temperature uniformity during tests. For each experiment, 20 g of tubers, were immersed in the beaker. During soaking, tubers were periodically removed, superficially dried with a tissue paper and weighed for water retention capacity up to attain an equilibrium value; i.e. when the increment change in sample weight was less than 0.01 g. At least three experiments were conducted for every solution concentration and soaking temperature.

The factors of variation of the treatments were:

- Nature of the solutions: calcium hydroxide, Kanwa and ascorbic acid.

- Concentrations of the solutions: 0; 0.1; 0.5; 1 and 1.5% (w/v) [11, 12, 15b].

- Temperatures of treatment: 20, 40 and 60°C [10, 15b].

The Water absorption capacity of the samples at each time (Ht) was calculated based on the increase in weight of the samples using Peleg model Eq. 1) [16, 17]:

$$H_t = H_o + \frac{t}{k_1 + k_2 t} \quad (1)$$

Where: Ht (% DW) is the moisture content at time t

H0 (% DW) is the initial moisture content

k1 (h.%⁻¹) is the Peleg rate constant which is related to absorption rate at the beginning of the process (t=t0)

k2 (%⁻¹) is the Peleg capacity constant related to maximum attainable moisture content (t→∞)

The model is linearized into the form:

$$\frac{t}{H_t - H_o} = k_1 + k_2 t \quad (2)$$

k1 and k2 constants are determined from the regression of t/(Ht - H0) vs. time.

Peleg constants (k1 and k2) values obtained were used in Eq. 2 [1, 15b] to calculate predicted values of moisture content (Hp) which were then compared to the corresponding experimental values in order to appreciate the goodness of fit of the Peleg model. The criterion used to evaluate the goodness of fit was the mean relative percentage deviation (p) between experimental data and theoretical values [18, 15b] calculated as:

$$p(\%) = \frac{100}{N} \sum_{i=1}^{N} \frac{|H_{ei} - H_{pi}|}{H_{ei}} \quad (3)$$

Where: Hei and Hpi are respectively experimental and predicted moisture content values. N is the number of experimental data. The model is considered acceptable if p values are below 10% [19, 20].

## Dehulling of tubers

At the end of soaking and swelling process, the dehulling consisted in removing the outer protective casing of the tuber by abrasion through scrubbing of the tubers between the hands. Dehulling efficiency was
calculated using the formula suggested by Doehlert and Wiessenborn [21] in which the mass percentage of dehulled tubers is compared to that of the whole tubers on dry matter basis.

To account for moisture change during soaking, the mass of tuber samples was measured before and after soaking and dehulling. Moisture correction factor (MFC) was calculated using Eq. 4.

$$MFC = \frac{W_t}{100-H} \qquad (4)$$

With: Wt (g): whole tuber mass with hulls
H (%): water content of the tubers

In addition, at the end of swelling and after dehulling, the hulls were weighed, dried at 105°C for 24 hours and reweighed to obtain a dry mass (Mh).

The dehulling efficiency is then given by the Eq. 5:

$$DHE (\%) = \frac{W_t-(M_h*MCF)}{W_t} * 100 \qquad (5)$$

Moreover, the solute losses during soaking at different temperatures were calculated according to Evranuz and Gürtas (22). A known quantity of soaking solution (v) was taken at the end of process, filtered using filter paper Whatman n°3, dried in oven at 105°C for 24 hours and weighed (m). The solute losses were expressed in g/100 g dry weight basis (Eq. 6).

$$SL = \frac{m+v*100*100}{(100-H)v*M} \qquad (6)$$

With: SL (g/100 g DW): Solute loss

m (g): mass of soluble obtained after drying of v aliquot

V (ml): total volume of soaking solution

v (ml): aliquot volume of the soaking solution

H (%): water content of the tubers

<figure><img src="image_4.png" /><figcaption>Figure 2: Water absorption of tiger nut tubers soaked in ascorbic acid (—), Ca(OH)2 (---) and Kanwa (.....) solutions at different temperatures.</figcaption></figure>

As the process continued, water absorption rate steadily decreased due to filling of water into free capillary and intermicellar increasing extraction rates of soluble solids from tubers. The absorption ceased when the tubers attained the equilibrium water content [1b].

The kinetics absorption of solution by black tiger nut tubers at various temperatures and concentrations of ascorbic acid, Kanwa and Ca(OH)₂

M (g): mass of tubers before soaking

## Microbiological analysis

The microbiological analyzes were performed according to standards methods (23). The research of the total microbial count was carried out by macerating tiger nut tubers in a sterile food processor. Ten g of each sample of tiger nut tubers (raw, soaked and dehulled) were crushed in a mortar in asepsis conditions. One g of the crushed sample was mixed with 9 mL of sterile physiological water solution (9 g of NaCl in 1 L of distilled water) and the mixture used for the preparation of decimal dilutions of 10⁻² to 10⁻⁹ and the highest three dilutions were taken for analysis.

Enumeration was carried out by counting of the colonies (CFU). The count was carried out in triplicates.

## Statistical analysis

All results are expressed as mean values of three separate determinations. The variation in the data collected and the statistical significance of the effect of the treatments were analyzed by the analysis of variance. The comparisons of means were made by Duncan's test at probability thresholds 1 and 5%. Statistical data was analyzed using STATGRAPHICS Centurion XVI.I (2010) software and Sigma Plot 11.0 was used to plot the curves

## Results and Discussion

### Water absorption of black variety of tiger nut tuber in ascorbic acid, Kanwa and calcium hydroxide solutions

Soaking is a long stage process in which the goal is the swelling of tiger nut tubers. During immersion, there is material transfer made both of soluble between tubers and the solution. For all the soaking solutions, tiger nut tubers showed typical sorption behavior with exponentially water content versus soaking time at all the temperatures (Fig. 2).

showed that the rate of absorption of water increased with the temperature of soaking like described by Djomdi et al. [1b]. Tables 1 and 2 show that no significant difference (p<0.05) was observed between the constants k₁ and the increase of the concentration whatever the treatment applied, as previously reported for tubers from brown variety [10, 15b].
<table><thead><tr><th rowspan="2">C (g/L)</th><th rowspan="2">T (°C)</th><th colspan="3">Ca(OH)<sub>2</sub></th><th colspan="3">Kanwa</th></tr><tr><th>k<sub>1</sub></th><th>k<sub>2</sub></th><th>r<sup>2</sup></th><th>P (%)</th><th>k<sub>1</sub></th><th>k<sub>2</sub></th><th>r<sup>2</sup></th><th>P (%)</th></tr></thead><tbody><tr><td rowspan="3">0</td><td>20</td><td>0.23±0.02<sup>a</sup></td><td>0.02</td><td>0.97</td><td>4.00</td><td>0.23±0.03<sup>a</sup></td><td>0.02</td><td>0.97</td><td>4.38</td></tr><tr><td>40</td><td>0.10±0.01<sup>b</sup></td><td>0.02</td><td>0.96</td><td>4.65</td><td>0.10±0.02<sup>b</sup></td><td>0.02</td><td>0.95</td><td>4.03</td></tr><tr><td>60</td><td>0.05±0.02<sup>c</sup></td><td>0.02</td><td>0.98</td><td>4.48</td><td>0.04±0.00<sup>c</sup></td><td>0.02</td><td>0.98</td><td>4.49</td></tr><tr><td rowspan="3">0.1</td><td>20</td><td>0.29±0.01<sup>a</sup></td><td>0.02</td><td>0.96</td><td>5.32</td><td>0.30±0.01<sup>a</sup></td><td>0.02</td><td>0.98</td><td>5.73</td></tr><tr><td>40</td><td>0.09±0.02<sup>b</sup></td><td>0.02</td><td>0.97</td><td>4.75</td><td>0.13±0.02<sup>b</sup></td><td>0.02</td><td>0.98</td><td>4.83</td></tr><tr><td>60</td><td>0.03±0.00<sup>c</sup></td><td>0.02</td><td>0.96</td><td>5.12</td><td>0.04±0.00<sup>c</sup></td><td>0.02</td><td>0.97</td><td>4.61</td></tr><tr><td rowspan="3">0.5</td><td>20</td><td>0.31±0.01<sup>a</sup></td><td>0.02</td><td>0.91</td><td>4.78</td><td>0.25±0.02<sup>a</sup></td><td>0.02</td><td>0.97</td><td>4.47</td></tr><tr><td>40</td><td>0.14±0.02<sup>b</sup></td><td>0.02</td><td>0.97</td><td>3.83</td><td>0.12±0.01<sup>b</sup></td><td>0.02</td><td>0.98</td><td>4.07</td></tr><tr><td>60</td><td>0.03±0.00<sup>c</sup></td><td>0.02</td><td>0.98</td><td>4.65</td><td>0.03±0.00<sup>c</sup></td><td>0.02</td><td>0.97</td><td>4.94</td></tr><tr><td rowspan="3">1</td><td>20</td><td>0.29±0.04<sup>a</sup></td><td>0.02</td><td>0.97</td><td>4.53</td><td>0.26±0.04<sup>a</sup></td><td>0.02</td><td>0.97</td><td>4.83</td></tr><tr><td>40</td><td>0.15±0.03<sup>b</sup></td><td>0.02</td><td>0.97</td><td>5.53</td><td>0.14±0.01<sup>b</sup></td><td>0.02</td><td>0.97</td><td>4.73</td></tr><tr><td>60</td><td>0.04±0.01<sup>c</sup></td><td>0.02</td><td>0.99</td><td>5.63</td><td>0.04±0.00<sup>c</sup></td><td>0.02</td><td>0.97</td><td>3.92</td></tr><tr><td rowspan="3">1.5</td><td>20</td><td>0.29±0.04<sup>a</sup></td><td>0.02</td><td>0.97</td><td>5.42</td><td>0.30±0.01<sup>a</sup></td><td>0.02</td><td>0.97</td><td>5.28</td></tr><tr><td>40</td><td>0.10±0.03<sup>b</sup></td><td>0.02</td><td>0.94</td><td>3.51</td><td>0.18±0.01<sup>b</sup></td><td>0.02</td><td>0.97</td><td>4.29</td></tr><tr><td>60</td><td>0.03±0.00<sup>c</sup></td><td>0.02</td><td>0.94</td><td>5.00</td><td>0.03±0.00<sup>c</sup></td><td>0.02</td><td>0.98</td><td>4.52</td></tr></tbody></table>

Means within the same column followed by same letter are not significantly different using one-way ANOVA test (P<0.05).

<caption>**Table 1:** Water absorption constants and goodness of fit of Peleg model of black variety of tigernut tubers soaked in alkaline solutions (Ca(OH)2 and Kanwa) at different temperatures.</caption>

However, a significant difference (p<0.05) between constancies k₁ and the increase of the temperature, whatever the treatment applied, was logically detected as an increasing temperature was correlated with a faster absorption of water reducing time of soaking (Fig. 2). The constant of Peleg (k₁) decreased with the increase of soaking temperature, whereas the constant of capacity of Peleg (k₂) was approximately 0.02% for all the conditions of soaking. There is a relative stability of the values of k₂ for all the temperatures and concentration of soaking. Djomdiet al. [10, 15b] reported that chemical modification of the tubers under the effect of the temperature (gelatinization) did not affect their capacity of water absorption. k₁ is a constant connected at the rate of transfer of the matter.

The weakness of the cellular membrane due to heating, increased the cellular permeability, involving for higher absorption solution by the tubers. This absorption was not constrained by the various treatments applied to the tubers during soaking. This phenomenon was observed by other authors and it was attributed to plasticizing effect of water at the temperature of starch gelatinization [10, 13-15, 24].

The fit of Equation (2) to absorption data showed that, at all soaking conditions, the degree of fit, judged by regression coefficient (r²), was about the same. Tables 1 and 2 provide the resulting Peleg constants (k₁ and k₂), r² and p values.

<table><thead><tr><th>C (g/L)</th><th>T (°C)</th><th>k<sub>1</sub></th><th>k<sub>2</sub></th><th>r<sup>2</sup></th><th>P (%)</th></tr></thead><tbody><tr><td rowspan="3">0</td><td>20</td><td>0.21<sup>a</sup></td><td>0.02</td><td>0.98</td><td>4.10</td></tr><tr><td>40</td><td>0.11<sup>b</sup></td><td>0.02</td><td>0.97</td><td>4.72</td></tr><tr><td>60</td><td>0.04</td><td>0.02</td><td>0.99</td><td>4.92</td></tr><tr><td rowspan="3">0.1</td><td>20</td><td>0.22<sup>a</sup></td><td>0.02</td><td>0.98</td><td>4.23</td></tr><tr><td>40</td><td>0.10<sup>b</sup></td><td>0.02</td><td>0.97</td><td>5.03</td></tr><tr><td>60</td><td>0.04<sup>c</sup></td><td>0.02</td><td>0.98</td><td>4.23</td></tr><tr><td rowspan="3">0.5</td><td>20</td><td>0.27<sup>a</sup></td><td>0.02</td><td>0.98</td><td>5.17</td></tr><tr><td>40</td><td>0.11<sup>b</sup></td><td>0.02</td><td>0.98</td><td>4.83</td></tr><tr><td>60</td><td>0.04<sup>c</sup></td><td>0.02</td><td>0.98</td><td>4.93</td></tr><tr><td rowspan="3">1</td><td>20</td><td>0.30<sup>a</sup></td><td>0.02</td><td>0.97</td><td>5.04</td></tr><tr><td>40</td><td>0.10<sup>b</sup></td><td>0.02</td><td>0.97</td><td>4.09</td></tr><tr><td>60</td><td>0.05<sup>c</sup></td><td>0.02</td><td>0.96</td><td>4.82</td></tr><tr><td rowspan="3">1.5</td><td>20</td><td>0.22<sup>a</sup></td><td>0.02</td><td>0.98</td><td>4.50</td></tr><tr><td>40</td><td>0.12<sup>b</sup></td><td>0.02</td><td>0.97</td><td>5.89</td></tr><tr><td>60</td><td>0.04<sup>c</sup></td><td>0.02</td><td>0.96</td><td>4.53</td></tr></tbody></table>

Means within the same column followed by same letter are not significantly different using one-way ANOVA test (P<0.05)

<caption>**Table 2.** Water absorption constants and goodness of fit of Peleg model of tiger nut tubers soaked in ascorbic acid at different temperatures</caption>
The use of kinetic data to determine the goodness of fit of the Peleg model resulted in a fit with r²≥0.90 at almost all soaking conditions (solutions concentrations, temperature and time). The relative deviation between experimental and predicted data is around 2.798–6.167 indicating that water absorption of tiger nut tubers during soaking in acid and alkali solution is well described by the Peleg model [25].

It should be noted that the diffusion was not done in one way as the tubers also lost solutes in the soaking solutions (fig. 3) meaning that soaking solutions were not sufficiently concentrated to prevent the opposite transfer of matter.

<figure><img src="image_5.png" /><figcaption>**Figure 3: Soluble solids loss of tiger nut tubers soaking at different temperatures**</figcaption></figure>

The losses of soluble solids from tubers at 20 and 40°C were less than 0.5 and 2% (w/w) respectively. Sayaret al. [14] stipulated that these values can be negligible compared to water gain. However at 60 °C 8.7% (w/w) of tuber solutes were recovered in soaking solutions. So, even if solutions at temperature of 60 °C and above accelerate the water absorption, they lead to significant damage in structure and tissue of tiger nut tubers. This phenomenon was not identified for ascorbic acid solutions indicating that higher concentration of osmolytes slowed down soluble solids loss even if higher temperature inactive and decompose ascorbic acid [26].

This part of work established the suitability of the model of Peleg to describe the behavior of the tubers of *C. esculentus*L. At the time of soaking [10, 15b]. The high temperatures accelerate the phenomenon of

absorption of water and loss of soluble solutes [15b]. Although the heating contributes to sterilize the tubers, with regard to the microbial load [27]. So the effects of the temperature and the nature of soaking solutions was investigated on the dehulling of tiger nut tubers.

## Dehulling efficiency of black tigernut tubers

The tough outer skin of tiger nut tubers consists of thin epidermis wrapped in the tegument [15b]. It is a very thin coat composed mainly of fibers and called fibro-collenchyma sleeve. This coat was removed by alkaline and acid action of soaking solutions [15b].

Fig. 4 shows dehulling efficiency of soaking solutions (acid and alkaline) at different temperatures.

<figure><img src="image_6.png" /><figcaption>**Figure 4: Dehulling efficiency of tiger nut tubers soaked at different temperatures in vitamin C, Ca(OH)2 and kanwa solutions**</figcaption></figure>
This figure shows that dehulling efficiency increased with increasing concentration of soaking solutions for all the three solutions tested. Significant variations in dehulling efficiency of solutions at 20 and 40°C was also detected [15b]. At these temperatures, ascorbic has the best dehulling efficiency for all the concentrations but at 60°C this activity is reduced compare to the two other solutions. This reduction activity can be attributed to temperature effect on ascorbic acid as explained previously by Marta et al. [26] and Djomdi et al. [15b]. Ascorbic acid exhibited the highest dehulling efficiency for all the temperature except at 60°C.

At the highest concentrations (1.5 g/L), most of the solutions had dehulling efficiency higher than 85%. Dehulling efficiency also increased with increasing temperatures ((< 65% at 20°C to > 70% at 60°C)) for tiger nut tubers soaked in water. It appeared most strongly affected by concentrations and temperatures of soaking solutions [15b].

## Microbial load of black tiger nut tubers before and after treatments

The control sample (raw tubers) had microbial load up to 10⁹ CFU/g, which is very higher compared to microbiological limits standards (10⁶–<10⁷ CFU/g) (28). Soaking at lower temperatures (20 and 40°C) and concentrations (0.1 and 0.5 g/L) increased microbial load of black tiger nut tubers varieties for the three solutions. Soaking in these conditions stimulated microbial growth by activating microorganisms as described by Shamsudden and Magashi, [29] and Djomdiet al. [15b]. On the contrary, high temperature led to a reducing of microbial load, but the greatest reduction was carried out by dehulling process [15b]. Figure 5 shows significant effect of dehulling on the microbial load of tiger nut tuber as for all treatments 2.5 log of microbial reduction was measured.

<figure><img src="image_7.png" /><figcaption>Figure 5: Microbial load of undehulled (—) and dehulled (---) tiger nut tubers soaked in vitamin C, Ca(OH)2 and Kanwa solutions</figcaption></figure>

This result is in agreement with the work of Muthomiet al. [30] and Bashir [31] who reported that semi- processed maize grains had lower microbial load.

Ascorbic acid solutions gave the best results in term of reduction of microbial load compared to the other soaking solutions [32]. This reduction is considerable at the higher temperatures, reaching a maximum of 4.8 log reduction [33, 34]. According to Marta et al. [26], the main product resulting from thermal ascorbic acid decomposition is dehydro-L-ascorbic acid. This compound seem then to have a similar effect as ascorbic acid in term of dehulling efficiency and microbial load reduction [35, 36].

Soaking and dehulling treatments led to tiger nut tuber effective decontamination in term of microbial load and good microbiological quality.

## Conclusion

The hydrothermal treatment of black tiger nut tubers variety in acid and alkaline solutions contributed to enhance dehulling efficiency and microbial load reduction. Since there was considerable reduction of microbial load at the higher temperature with ascorbic acid based soaking treatments. These treatments led to important soluble solids loss, which compromising nutritional and organoleptic qualities of tiger nut tubers.

The impact of ascorbic acid on dehulling efficiency and microbial load demonstrated that research must be continued with regard for the using of alternative acid solutions like acetic acid, lemon extracts for lowering this processing cost like on brown varieties tubers.

## References

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