# From animal to plant ecosystems and vice versa

Isabelle Virlogeux-Payant, Matthieu Barret

► To cite this version:

Isabelle Virlogeux-Payant, Matthieu Barret. From animal to plant ecosystems and vice versa. 2nd Management Committee & Working Group Meeting of COST Action CA16110 HUPlant Control, Jun 2017, Amersfoort, Netherlands. hal-01608168

HAL Id: hal-01608168

https://hal.science/hal-01608168

Submitted on 5 Jun 2020

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INRA
SCIENCE & IMPACT

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Institut de Recherche en
Horticulture et Semences

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université
angers

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EMERSYS

Emergence, systématique
et écologie des bactéries
associées aux plantes

From animal to plant ecosystems and vice versa ?

Isabelle Virlogeux-Payant

INRA, UMR1282 Infectiology and Public Health
« Signaling, Bacterial Carriage and Virulence » team

Matthieu Barret

IRHS, Research Institute of Horticulture and Seeds
Emergence, systematics and ecology of plant-associated bacteria

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INRA
SCIENCE & IMPACT

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One Health concept

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EMERSYS

Emergence, systématique
et écologie des bactéries
associées aux plantes

Animal Health

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pathogen

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Human Health

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Food safety and security

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Environmental Health

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INRA

SCIENCE & IMPACT

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Team « EmerSys »

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Understand biological/ecological processes involved in seed transmission of microbes

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- Pathogen-centric approach (Xanthomonas)

- Seed transmission pathways (systemic, floral)

- Bacterial determinants (T3SS, adhesins, ...) involved in transmission

- Seed-associated microbial communities (various plant sp.)

- Ecological processes involved in assembly of seed microbiota

- Dynamics of seed microbiota during germination and emergence of seedling

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Goal : understand Resistance/resilience of seed microbiota in order to control seed-transmitted pathogens
# Team « EmerSys »

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## Skills

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## Microbial Ecology

- Barcoding (16S rRNA gene, ITS1, *gyrB*)

- Metagenomics (taxonomic classif of reads, gene-centric, binning)

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## Taxonomy

- Strain collection (CIRM-CFBP : 7000 bacterial strains)

- Phylogeny/Phylogenomics/Pop. genomics

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## Phytopathology (plant-bacterial interactions)

- Greenhouses and growth chamber (S2-S3)

- Plant phenotyping facility (Phenotics) → impact of plant pathogen on various host plants
# Team « EmerSys »

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## Interest in COST HuPlant control

- Response of the seed microbiota to invasion by HPMO (WG1)

- Seed transmission pathways of HPMO

- Taxonomic classification of HPMO (WG2)

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# Team « Signaling, Carriage and Bacterial virulence » topics

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# Team « Signaling, Carriage and Bacterial virulence » skills

## Bacterial genetics

- Mutants in SE, STM

- All genetic manipulations

- qRT-PCR

- Barcode-tagged mutant library in STM14028 under characterization

- Constitutively bioluminescent or fluorescent *Salmonella*

- Gene fusions with *lux*, *lacZ*, fluorescence genes

Characterization of virulence factors and their regulation

I. Virlogeux-Payant,
O. Grépinet

New invasion factors

Cell invasion and intracellular behaviour

mechanisms:
Rck invasin

A. Wiedemann
S. Holbert

**Salmonella:**

**of virulence and carrier state**

and the serotype, *Salmonella* can induce:
> than 27 millions people in the world
> 94 millions of human cases worldwide
> source of human contamination via

contaminated food (poultry, pig).

**Pathogenesis key step: host cell invasion**

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Immune response

A.M. Chaussé

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**in vivo mechanisms:**
Virulence, carrier state, transmission

Ph. Velge

In vivo infected cells

S. Roche

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# Team « Signaling, Carriage and Bacterial virulence » skills

## Cellular Microbiology

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# Team « Signaling, Carriage and Bacterial virulence » skills
## Animal models and immune response

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INRA
SCIENCE & IMPACT

# Team « Signaling, Carriage and Bacterial virulence »

## COST HuPlant control interest

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INRA
SCIENCE & IMPACT

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INRA
SCIENCE & IMPACT

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EMERSYS
Emergence, systématique
et écologie des bactéries
associées aux plantes

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INRA
SCIENCE & IMPACT

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