# A transcriptomic approach identifies candidate genes for drought tolerance during the reproductive phase in pea

Vanessa Vernoud, Nadia Rossin, Marion Prudent, Christine Le Signor, Myriam Sanchez, Stephanie Pateyron, Sandrine Balzergue, Gregoire Aubert, Judith Burstin, Karine Gallardo-Guerrero, et al.

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## To cite this version:

Vanessa Vernoud, Nadia Rossin, Marion Prudent, Christine Le Signor, Myriam Sanchez, et al. A transcriptomic approach identifies candidate genes for drought tolerance during the reproductive phase in pea. 2. International Legume Society Conference - Legumes for a Sustainable World, Legume Society., Oct 2016, Lisbonne, Portugal. 358 p. hal-01604112

HAL Id: hal-01604112

https://hal.science/hal-01604112

Submitted on 3 Jun 2020

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# Legumes for a Sustainable World
## ILS2 | Second International Legume Society Conference

11th - 14th OCT 2016

TRÓIA RESORT | PORTUGAL

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UNIVERSIDADE
NOVA
DE LISBOA

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A transcriptomic approach identifies candidate genes for drought tolerance during the reproductive phase in pea.

Vernoud V.¹, Rossin N.¹, Prudent M.¹, Le Signor C.¹, Sanchez, M.¹,
Pateyron S.², Balzergues S.², Aubert G.¹, Burstin J.¹, Gallardo K.¹,
Thompson R.¹

1 - Agroécologie, AgroSup Dijon, CNRS, INRA, Univ. Bourgogne Franche-Comté, F-21000 Dijon, France.

2 - POPS Transcriptomic Platform, IPS2, CNRS, INRA, Univ 12 Paris Sud, Univ Evry, Univ Paris-Diderot, Sorbonne Paris-Cite, Univ Paris-Saclay, 91405 Orsay, France

Drought is a major environmental factor limiting the productivity of
crop plants. In pea drought stress occurring during the reproductive
phase can greatly affect seed yield and quality. We investigated the
response of pea plants (var. Caméor) subjected to water stress during
the seed filling period, a phase when massive remobilization from the
vegetative organs occurs to sustain seed high-nitrogen demand. Pea
plants were subjected to drought stress at the beginning of the seed-
filling period of the first two nodes for 8 days. Total and one-seed
biomass decreased by 35% and 20% respectively by this limited water
stress. Nitrogen allocation to the different plant compartments was
also affected, with an increased N allocation to the leaves from the
vegetative nodes and a decrease to the root and seed compartments.
Transcriptomic changes in water-stressed leaves from the vegetative
nodes were analysed by hybridization of a 40k pea micro-array: 178
genes were at least two fold up-regulated in water-stressed samples
compared to well-watered samples, whereas 55 genes were down-
regulated. Among the most strongly up-regulated genes were those
encoding a glutamine amidotransferase, a sucrose transporter from
the SWEET family, a phosphoenolpyruvate carboxykinase and a
carotenoid cleavage dioxygenase involved in strigolactone
biosynthesis.

Acknowledgement: this project was supported by the Burgundy Region (AGRALE6, FABER program) and the FP7 LEGATO project.