# Quantitative Measurement of the Chemical Composition of Fatty Acid of Cocoa Butter and the Isotopic Content of Glycerol Contained in Cocoa Butter by the NMR 13 C from the INEPT Sequence and Characterization of the Geographical Origin of the Cocoa

Didier Diomandé, Virginie Silvestre, Tiemoko Touré Dro, Jacques Sagne

Akpa, Illa Tea, Gérald Remaud

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Didier Diomandé, Virginie Silvestre, Tiemoko Touré Dro, Jacques Sagne Akpa, Illa Tea, et al.. Quantitative Measurement of the Chemical Composition of Fatty Acid of Cocoa Butter and the Isotopic Content of Glycerol Contained in Cocoa Butter by the NMR 13 C from the INEPT Sequence and Characterization of the Geographical Origin of the Cocoa. American Journal of Analytical Chemistry, 2022, 13 (03), pp.79-95. 10.4236/ajac.2022.133007. hal-05099971

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# Quantitative Measurement of the Chemical Composition of Fatty Acid of Cocoa Butter and the Isotopic Content of Glycerol Contained in Cocoa Butter by the NMR ¹³C from the INEPT Sequence and Characterization of the Geographical Origin of the Cocoa

Didier Diomandé<fnref n="1" />,<fnref n="2" />*, Tiemoko Touré Dro<fnref n="3" />, Jacques Sagne Akpa<fnref n="1" />,
Silvestre Virginie<fnref n="2" />, Illa Tea<fnref n="2" />, Gérald S. Remaud<fnref n="2" />

Email: *dggbe@yahoo.fr

**How to cite this paper:** Diomandé, D., Dro, T.T., Akpa, J.S., Virginie, S., Tea, I. and Remaud, G.S. (2022) Quantitative Measurement of the Chemical Composition of Fatty Acid of Cocoa Butter and the Isotopic Content of Glycerol Contained in Cocoa Butter by the NMR ¹³C from the INEPT Sequence and Characterization of the Geographical Origin of the Cocoa.
*American Journal of Analytical Chemistry*, **13**, 79-95.

https://doi.org/10.4236/ajac.2022.133007

Received: November 23, 2021

Accepted: March 4, 2022

Published: March 7, 2022

Copyright © 2022 by author(s) and
Scientific Research Publishing Inc.
This work is licensed under the Creative
Commons Attribution International
License (CC BY 4.0).

http://creativecommons.org/licenses/by/4.0/

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Open Access

## Abstract

The determination of the chemical composition of cocoa butter and the isotopic contents of the carbons (C-2, C-1 and C-3) of the glycerol contained in the cocoa butter were measured by the NMR-¹³C from the INEPT sequence. This technique has already been applied to triglycerides of animal origin. Thus, cocoa butter is made up of nearly 66% saturated fatty acid composed mainly of stearic acid and palmitic acid. Monounsaturated fatty acids represented by oleic acid have an average content of 31% and polyunsaturated fatty acids represent less than 4%. Likewise, the isotopic contents of the glycerol of the triglycerides of cocoa butter in ¹³C of the carbons C-2, C-1 and C-3 were measured directly and values vary from -11‰ to -19‰ for the C-2 and from -39‰ to -41‰ for all C-1 and C-3 because of the symmetry of glycerol.

## Keywords

Chemical Composition, Cocoa Butter, INEPT, Isotopic Content

## 1. Introduction

Lipids are produced by plants and animals, and they constitute the fat of living

<footnote n="1">¹Laboratoire de Constitution et Réaction de la Matière de l'Université Félix Houphouët-Boigny, Abidjan, Côte d'Ivoire</footnote>

<footnote n="2">²Chimie et Interdisciplinarité, Synthèse, Analyse, Modélisation (CEISAM), Nantes, France</footnote>

<footnote n="3">³Laboratoire des Sciences de la Matière, de l'Environnement et de l'énergie Solaire, Université Félix Houphouët-Boigny, Abidjan,
Côte d'Ivoire</footnote>
things [1]. Lipids are used in cosmetics, pharmaceuticals and human food. Lipids are constituents of cell membranes, inter and intracellular messengers and an energy reserve for the human body [2] [3]. The form of lipids present in the body is that of fatty acid triglycerides. Fatty acids can be divided into three classes: saturated, monounsaturated and polyunsaturated. Unsaturated fatty acids, in particular oleic acid and linoleic acid, play an essential nutritional role. They have a preventive action against cardiovascular disease by lowering cholesterol levels, which is not the case for certain saturated fatty acids [4]. The chemical composition in fatty acids is linked to the botanical or animal origin or to the processes used to obtain it, but also to the geographical origin [5]. Today's society is demanding on the origin of the products consumed. Thus each actor, from the product to the consumer, wants the origin of the animal or vegetable fats used to be guaranteed, whether in a raw or processed product, in order to control its quality. It is therefore in this sense that this work is part of the determination of the fatty acid composition but also of the determination of the ¹³C isotopic signature of the glycerol contained in cocoa butter by the ¹³C-INEPT method. This technique has already made it possible to analyze triacylglycerols in olive oil and also to quantify several fatty acids, triglycerols in egg yolks [6].

## 2. Materials and Methods

### 2.1. Preparation of the Sample

We worked on 41 samples from 21 producing countries. These samples were provided by the International Agricultural Research Center for Development (CIRAD) and those from Côte d'Ivoire were obtained from farmers in different producing regions. Cocoa butter is extracted from the cocoa beans separated from the shells. 10 g of cotyledons are finely ground and the powder obtained is extracted hot (90°) at reflux, for 2 hours, with (3 × 100 mL) of cyclohexane (Prolabo). Other solvents such as hexane, acetone can also be used for the extraction of cocoa butter. After cooling and filtration, the residue constitutes lean cocoa. The filtrate is evaporated and gives cocoa butter (45% to 50%). After separation on a vacuum pump to remove traces of cyclohexane, a mass of 403.2 mg of cocoa butter is dissolved in 630 mg of $CDCl_3$ (Eurisotop). The solution is well homogenized and then introduced into a 5 mm diameter NMR tube.

### 2.2. Isotopic ¹³C NMR Acquisition Parameters

Carbon has two isotopes, ¹²C, which has an isotopic abundance of about 98.9%, and ¹³C, which is only 1.1%. In the ¹³C NMR study, the spectrum obtained gives low intensities, which does not allow spectrum analysis. In order to obtain a spectrum favorable to the analysis, polarization transfers from hydrogen to carbon-13 are carried out, among the polarization transfer techniques we use INEPT (Insensitive Nuclei Enhanced by Polarization Transfer). The INEPT or Insensitive Core Reinforced by Polarization Transfer provides a spectrum favorable to analysis by proceeding by polarization transfer from ¹H to ¹³C. In
INEPT, the variation of the pulse angle depends on the chemical shift and the $J_{C-H}$ decoupling constant, hence the presence of signal distortion caused by different couplings present. The principle can be broken down as follows, we have the ¹H excitation which firstly allows the polarization to be transferred from ¹H to ¹³C and secondly to detect information on the ¹³C nucleus. The start of this sequence is a spin echo that inverts the population of a subpopulation of protons resulting in carbon selectivity. This results in a gain in intensity of the ¹³C signals. The linear combinations of these different spectra then make it possible to separate the CH, CH₂ and CH₃ sub-spectra. However, this assumes that all the groups have identical (or in any case very close) $J_{C-H}$ coupling constants, which is rarely verified.

The ¹³C-INEPT method has already been discussed in the CEISAM “Chemistry and Interdisciplinarity, Synthesis, Analysis, Modeling” laboratory in the EBSI team under these fundamental and application aspects [7]. The application with the resulting cocoa butter was carried out in collaboration with the NMR specialists of the EBSI team. The ¹³C NMR experiments were carried out on the 500 MHz Bruker Avance III spectrometer equipped with a ¹³C/¹H dual cryo probe with ²H lock. All the experiments were carried out at a temperature of 293 K and without rotating the tubes.

The ¹³C NMR isotopic analysis of the glycerol of the triglycerides of cocoa butter was also carried out using a ¹³C acquisition sequence, decoupled ¹H consisting of a recovery time followed by the application of an excitation pulse of which 1 The rocking angle is equal to 90° and decoupling ¹H during the collection of the FID only (zgig sequence). This sequence will serve as a reference to calibrate the results obtained with the ¹³C-INEPT sequence. The parameters used for this measurement are as follows:

* Signal acquisition time (AQ): 1 s and $T_{1max}$ = 3.6 s

* Time between two passages (D1): 42 s (TR = D1 + AQ = 43 s > 10 $T_{1max}$)

* Number of passages (NS): 72 with a S/N ratio ≥ 500

The duration of the isotopic analysis of the glycerol of the triglycerides of cocoa butter being relatively long (55 min per spectrum), a polarization transfer method making it possible to increase the sensitivity and therefore to reduce the analysis time for the same S/B was used (12 min per spectrum). The transfer method that was chosen is a refocused INEPT (Insensitive Nuclei Enhancement by Polarization Transfer) in which the 180° inversion and refocusing pulses are adiabatic pulses [8] (Figure 1). The parameters of this sequence and more particularly the delays of the two spin echoes were optimized so as to obtain the same δ¹³C (‰) as those obtained by the zgig sequence on the 2 sites relating to the glycerol of the triglycerides of cocoa butter. The optimized parameters for this refocused INEPT-sequence are as follows:

* Signal acquisition time (AQ): 1 s and $T_{1max}$ = 2.8 s

* Time between two passages (D1): 24 s (TR = D1 + AQ = 25 s ≥ 8 $T_{1max}$)

* Times $t_1$ = 2.7 msec and $t_2$ = 1.405 msec – Number of passages (NS): 24 with
<figure><img src="image_3.png" /><figcaption>**Figure 1.** $^{13}$C-INEPT-refocused sequence in which the 180° inversion and refocus are adiabatic pulses.</figcaption></figure>

a S/N ratio ≥ 800.

For the two sequences, 5 spectra were carried out. However, all the spectra carried out on the cocoa butter samples were obtained with the refocused INEPT-sequence, only on one of the samples of the series the zgig sequence was operated in order to ensure that we always obtained the same results. The acquired $^{13}$C NMR spectra were subsequently processed with a decreasing exponential type apodization function using a line broadening factor (lb) of 1.5 Hz. The baseline correction was made by part with a polynomial of degree 5. The area of the different lines present on the NMR-$^{13}$C spectra was determined using the Perch software (University of Kuopio, Finland). The different components of the lines corresponding to the carbon sites relating to glycerol and to the fatty acids of the triglycerides of cocoa butter were deconvoluted by Lorentzian lines whose chemical shifts, the width at mid-height and the amplitude of the peaks were adjusted. In the case of the measurements obtained with the zgig sequence our results are perfectly validated, in the case of the INEPT-refocused sequence, by the sequence itself the areas of the signals are not only proportional to the number of $^{13}$C nuclei [9]. Thus, an average corrective factor established on the results of the 9 samples measured with the zgig sequence was applied to the areas obtained with the INEPT-refocused sequence.

# 3. Results and Discussion

## 3.1. Application to Glycerol and Fatty Acids of the Triglycerides of Cocoa Butter

There are two types of information that can be obtained from the $^{13}$C-NMR spectra acquired with the zgig and refocused INEPT sequences: on the one hand, information on the $\delta^{13}$C (‰) isotopic deviations of the two observable sites due to the symmetry of the glycerol (C-1, C-3 and C-2) and on the other hand information on the fatty acid composition of triglycerides in cocoa butter. Indeed, the NMR-$^{13}$C spectrum of cocoa butter is divided into 4 regions (Figure 2):

* The carbonyl region from 165 to 180 ppm

* The region of olefinic carbons from 120 to 140 ppm

* The glycerol region from 55 to 74 ppm

* The region of aliphatic carbons from 10 to 40 ppm
<figure><img src="image_4.png" /><figcaption>Figure 2. ¹³C NMR spectrum (zgig sequence) of cocoa butter in CDCl₃ on the 500 MHz spectrometer.</figcaption></figure>

Each of these regions can provide us with information of a different nature and/or validate the results obtained on other areas of the spectrum, a complete allocation of each of the regions was therefore carried out according to the literature (Figure 3). The number following the name of the esterified fatty acid chain corresponds to the carbon number according to the classical nomenclature used to describe fatty acids. As a reminder, the general chemical formula of triglycerides is shown in Figure 4. The carbon chains R1, R2 and R3 are fatty acids. The three fatty acids are not necessarily the same. The position on the glycerol backbone of the fatty acid chains is designated by the numbering position 1, 2 and 3. In triglycerides, the fatty acid chains can have 4 to 32 carbon atoms, but those of 16 and 18 atoms are the most common lengths. Triglycerides can be saturated, monounsaturated, or polyunsaturated. The fatty acids mainly contained in cocoa butter are: oleic, palmitic and stearic acid.

In the case of the measurements obtained with the zgig sequence our results are perfectly validated, in the case of the refocused INEPT-sequence, by the sequence itself the areas of the signals are not only proportional to the number of ¹³C nuclei [9]. Thus, an average corrective factor established on the results of the 9 samples measured with the zgig sequence was applied to the areas obtained with the refocused INEPT-sequence. The chemical compositions obtained from the cocoa butters, as well as the isotopic deviations determined on the glycerol contained in the cocoa butters are given in Table 1.

## 3.2. Statistical Treatment of the Data Obtained

Analysis of these results shows that the chemical fatty acid composition of cocoa
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<figure><img src="image_9.png" /><figcaption>**Figure 3.** Assignment of the different areas of the ¹³C NMR spectrum of cocoa butter. The signals corresponding to the chains of fatty acids esterified at position 1, 3 or 2 of glycerol are indicated as follows: L (Linoleyl), Ln (Linolenyl), O (Oleyl), P (Palmityl) and S (Stearyl), the position of the esterification of the fatty acid chain on glycerol is indicated.</figcaption></figure>

<figure><img src="image_10.png" /><figcaption>**Figure 4.** General chemical formula of triglycerides.</figcaption></figure>

butter is predominantly made up of saturated fatty acids, followed by the monounsaturated ones. Thus, we have nearly 63% to 68% of saturated fatty acids composed mainly of stearic acid and palmitic acid, while monounsaturated fatty acids (oleic acid) represent 30% to 32%. Polyunsaturated acids represent less
<table><caption>**Table 1.** Chemical composition of fatty acids in cocoa butter and the δ¹³C-2 and δ¹³C-1-3 isotopic signatures of the glycerol contained in cocoa butter.</caption><thead><tr><th rowspan="2">Origines</th><th colspan="2">% du linoléique à partir du C16</th><th colspan="2">% des oléiques 1-3 = O10</th><th colspan="2">% des oléiques 2 = O10</th><th colspan="2">% des linoléiques 1-3 = L10</th></tr><tr><th>Mean</th><th>Standard deviation</th><th>Mean</th><th>Standard deviation</th><th>Mean</th><th>Standard deviation</th><th>Mean</th><th>Standard deviation</th></tr></thead><tbody><tr><td>Abengourou (21)</td><td>2.46</td><td>0.03</td><td>9.44</td><td>0.35</td><td>90.69</td><td>0.32</td><td>14.53</td><td>1.00</td></tr><tr><td>Aboisso (21)</td><td>2.58</td><td>0.03</td><td>6.61</td><td>0.57</td><td>93.28</td><td>0.52</td><td>8.03</td><td>1.10</td></tr><tr><td>Agboville (21)</td><td>2.44</td><td>0.02</td><td>5.56</td><td>0.12</td><td>94.23</td><td>0.11</td><td>10.32</td><td>0.96</td></tr><tr><td>Alepé (21)</td><td>2.62</td><td>0.02</td><td>10.39</td><td>0.77</td><td>89.82</td><td>0.70</td><td>10.34</td><td>1.18</td></tr><tr><td>Azaguié (21)</td><td>2.37</td><td>0.01</td><td>7.55</td><td>0.48</td><td>92.42</td><td>0.44</td><td>11.65</td><td>0.89</td></tr><tr><td>Biankouma (21)</td><td>2.40</td><td>0.01</td><td>6.93</td><td>0.37</td><td>92.99</td><td>0.34</td><td>11.24</td><td>1.38</td></tr><tr><td>Bolivie (1)</td><td>2.12</td><td>0.01</td><td>8.59</td><td>1.35</td><td>91.47</td><td>1.24</td><td>16.72</td><td>0.84</td></tr><tr><td>Brésil (2)</td><td>3.23</td><td>0.01</td><td>11.52</td><td>1.55</td><td>88.79</td><td>1.41</td><td>12.80</td><td>1.27</td></tr><tr><td>Cameroun (3)</td><td>2.23</td><td>0.01</td><td>16.18</td><td>0.10</td><td>84.54</td><td>0.09</td><td>23.23</td><td>2.20</td></tr><tr><td>Colombie (4)</td><td>3.29</td><td>0.03</td><td>8.16</td><td>0.20</td><td>91.86</td><td>0.19</td><td>11.70</td><td>0.18</td></tr><tr><td>Costa Rica (5)</td><td>2.88</td><td>0.02</td><td>9.62</td><td>0.93</td><td>90.52</td><td>0.85</td><td>11.82</td><td>1.11</td></tr><tr><td>Dabou (21)</td><td>2.82</td><td>0.03</td><td>4.27</td><td>0.28</td><td>95.41</td><td>0.26</td><td>8.73</td><td>0.46</td></tr><tr><td>Daloa (21)</td><td>2.96</td><td>0.03</td><td>9.93</td><td>0.43</td><td>90.24</td><td>0.39</td><td>12.50</td><td>0.63</td></tr><tr><td>Danané (21)</td><td>2.53</td><td>0.03</td><td>6.33</td><td>0.26</td><td>93.53</td><td>0.24</td><td>8.50</td><td>1.04</td></tr><tr><td>Dimbokro (21)</td><td>2.46</td><td>0.02</td><td>7.49</td><td>2.40</td><td>92.48</td><td>2.19</td><td>14.68</td><td>1.32</td></tr><tr><td>Divo (21)</td><td>2.44</td><td>0.01</td><td>6.48</td><td>0.27</td><td>93.39</td><td>0.25</td><td>11.23</td><td>2.34</td></tr><tr><td>Douroubé (21)</td><td>2.60</td><td>0.01</td><td>10.41</td><td>0.41</td><td>89.81</td><td>0.37</td><td>13.45</td><td>1.53</td></tr><tr><td>Duékoué (21)</td><td>2.57</td><td>0.02</td><td>6.37</td><td>0.36</td><td>93.50</td><td>0.33</td><td>12.61</td><td>1.34</td></tr><tr><td>Equateur (6)</td><td>2.40</td><td>0.02</td><td>9.04</td><td>0.38</td><td>91.06</td><td>0.35</td><td>10.36</td><td>0.44</td></tr><tr><td>Gabon (7)</td><td>3.08</td><td>0.02</td><td>12.55</td><td>0.76</td><td>87.86</td><td>0.70</td><td>13.01</td><td>1.39</td></tr><tr><td>Ghana (8)</td><td>2.66</td><td>0.01</td><td>11.31</td><td>1.60</td><td>88.99</td><td>1.46</td><td>13.72</td><td>1.56</td></tr><tr><td>Guatemala (9)</td><td>2.17</td><td>0.03</td><td>15.12</td><td>1.39</td><td>85.51</td><td>1.27</td><td>23.03</td><td>1.16</td></tr><tr><td>Gueyo (21)</td><td>2.63</td><td>0.02</td><td>6.51</td><td>1.56</td><td>93.37</td><td>1.42</td><td>8.49</td><td>1.66</td></tr><tr><td>Guiglo (21)</td><td>2.61</td><td>0.03</td><td>11.66</td><td>1.41</td><td>88.66</td><td>1.29</td><td>11.38</td><td>1.46</td></tr><tr><td>Indonésie (10)</td><td>2.57</td><td>0.04</td><td>9.06</td><td>1.27</td><td>91.05</td><td>1.16</td><td>13.84</td><td>1.25</td></tr><tr><td>Jamaïque (11)</td><td>3.15</td><td>0.03</td><td>15.27</td><td>1.17</td><td>85.38</td><td>1.07</td><td>14.14</td><td>0.88</td></tr><tr><td>Kouibly (21)</td><td>2.57</td><td>0.03</td><td>5.83</td><td>0.21</td><td>93.99</td><td>0.19</td><td>11.28</td><td>2.05</td></tr><tr><td>Madagascar (12)</td><td>2.07</td><td>0.02</td><td>7.70</td><td>0.27</td><td>92.28</td><td>0.25</td><td>16.78</td><td>1.42</td></tr><tr><td>Malaisie (13)</td><td>2.65</td><td>0.02</td><td>9.11</td><td>1.71</td><td>90.99</td><td>1.56</td><td>10.59</td><td>1.07</td></tr><tr><td>Man (21)</td><td>2.93</td><td>0.03</td><td>9.33</td><td>0.35</td><td>90.79</td><td>0.32</td><td>10.97</td><td>1.18</td></tr><tr><td>Martinique (14)</td><td>2.66</td><td>0.02</td><td>6.31</td><td>0.25</td><td>93.55</td><td>0.23</td><td>13.83</td><td>1.60</td></tr></tbody></table>
Continued

<table><tr><td>Oumé (21)</td><td>2.59</td><td>0.03</td><td>7.58</td><td>0.37</td><td>92.39</td><td>0.34</td><td>15.41</td><td>1.20</td></tr><tr><td>Pérou (15)</td><td>2.53</td><td>0.03</td><td>6.39</td><td>0.27</td><td>93.48</td><td>0.25</td><td>14.57</td><td>3.93</td></tr><tr><td>PNG (16)</td><td>2.31</td><td>0.01</td><td>3.97</td><td>0.25</td><td>95.68</td><td>0.23</td><td>1218</td><td>1.14</td></tr><tr><td>REP. Dominicaine (17)</td><td>3.13</td><td>0.02</td><td>16.73</td><td>0.56</td><td>84.04</td><td>0.51</td><td>19.39</td><td>0.64</td></tr><tr><td>San Pedro (21)</td><td>2.70</td><td>0.01</td><td>9.47</td><td>0.26</td><td>90.67</td><td>0.23</td><td>50.39</td><td>0.29</td></tr><tr><td>Soubré (21)</td><td>2.50</td><td>0.03</td><td>8.02</td><td>0.27</td><td>91.99</td><td>0.25</td><td>13.19</td><td>0.83</td></tr><tr><td>Trinidad (18)</td><td>2.68</td><td>0.03</td><td>4.63</td><td>0.30</td><td>95.08</td><td>0.28</td><td>12.59</td><td>1.57</td></tr><tr><td>Venezuela (19)</td><td>2.73</td><td>0.03</td><td>13.20</td><td>0.15</td><td>87.26</td><td>0.14</td><td>20.75</td><td>1.16</td></tr><tr><td>Vietnam (20)</td><td>2.27</td><td>0.02</td><td>6.54</td><td>0.13</td><td>93.34</td><td>0.12</td><td>9.49</td><td>1.20</td></tr><tr><td>Yakro (21)</td><td>1.99</td><td>0.02</td><td>10.43</td><td>0.22</td><td>89.79</td><td>0.20</td><td>17.61</td><td>4.18</td></tr></table>

<table><thead><tr><th rowspan="2">Origines</th><th colspan="2">% des linoléiques 2 = L10</th><th colspan="2">% Insaturés (O + L + Ln)</th><th colspan="2">% des polyinsaturés</th><th colspan="2">% linolénique Ln16</th></tr><tr><th>Mean</th><th>Standard deviation</th><th>Mean</th><th>Standard deviation</th><th>Mean</th><th>Standard deviation</th><th>Mean</th><th>Standard deviation</th></tr></thead><tbody><tr><td>Abengourou</td><td>95.22</td><td>1.18</td><td>33.91</td><td>0.02</td><td>2.67</td><td>0.03</td><td>0.15</td><td>0.03</td></tr><tr><td>Aboisso</td><td>102.93</td><td>1.30</td><td>34.45</td><td>0.03</td><td>2.88</td><td>0.02</td><td>0.15</td><td>0.03</td></tr><tr><td>Agboville</td><td>100.21</td><td>1.14</td><td>33.37</td><td>0.03</td><td>2.78</td><td>0.18</td><td>0.14</td><td>0.03</td></tr><tr><td>Alepé</td><td>100.18</td><td>1.40</td><td>33.47</td><td>0.02</td><td>2.83</td><td>0.02</td><td>0.15</td><td>0.02</td></tr><tr><td>Azaguié</td><td>98.64</td><td>1.05</td><td>33.48</td><td>0.01</td><td>2.58</td><td>0.03</td><td>0.15</td><td>0.02</td></tr><tr><td>Biankouma</td><td>99.12</td><td>1.64</td><td>33.83</td><td>0.03</td><td>2.63</td><td>0.01</td><td>0.14</td><td>0.03</td></tr><tr><td>Bolivie</td><td>92.62</td><td>0.99</td><td>34.58</td><td>0.02</td><td>2.47</td><td>0.02</td><td>0.17</td><td>0.03</td></tr><tr><td>Brésil</td><td>97.27</td><td>1.50</td><td>35.48</td><td>0.01</td><td>3.49</td><td>0.02</td><td>0.18</td><td>0.03</td></tr><tr><td>Cameroun</td><td>84.91</td><td>2.60</td><td>33.15</td><td>0.01</td><td>2.52</td><td>0.03</td><td>0.12</td><td>0.02</td></tr><tr><td>Colombie</td><td>98.58</td><td>0.22</td><td>34.09</td><td>0.01</td><td>3.51</td><td>0.03</td><td>0.18</td><td>0.02</td></tr><tr><td>Costa Rica</td><td>98.43</td><td>1.31</td><td>35.29</td><td>0.01</td><td>3.15</td><td>0.04</td><td>0.20</td><td>0.03</td></tr><tr><td>Dabou</td><td>102.10</td><td>0.54</td><td>33.56</td><td>0.02</td><td>3.05</td><td>0.01</td><td>0.17</td><td>0.02</td></tr><tr><td>Daloa</td><td>97.62</td><td>0.75</td><td>35.25</td><td>0.03</td><td>3.14</td><td>0.02</td><td>0.18</td><td>0.02</td></tr><tr><td>Danané</td><td>102.36</td><td>1.23</td><td>33.77</td><td>0.02</td><td>2.99</td><td>0.34</td><td>0.14</td><td>0.02</td></tr><tr><td>Dimbokro</td><td>95.04</td><td>1.57</td><td>33.01</td><td>0.02</td><td>2.62</td><td>0.02</td><td>0.12</td><td>002</td></tr><tr><td>Divo</td><td>99.14</td><td>2.78</td><td>33.57</td><td>0.02</td><td>2.68</td><td>0.03</td><td>0.14</td><td>002</td></tr><tr><td>Douroubé</td><td>96.50</td><td>1.81</td><td>33.52</td><td>0.03</td><td>2.75</td><td>0.01</td><td>0.12</td><td>002</td></tr><tr><td>Duékoué</td><td>97.50</td><td>1.59</td><td>33.50</td><td>0.03</td><td>2.86</td><td>0.12</td><td>0.18</td><td>003</td></tr><tr><td>Equateur</td><td>100.16</td><td>0.52</td><td>33.96</td><td>0.02</td><td>2.71</td><td>0.02</td><td>0.17</td><td>002</td></tr><tr><td>Gabon</td><td>97.02</td><td>1.64</td><td>36.14</td><td>0.03</td><td>3.32</td><td>0.03</td><td>0.16</td><td>004</td></tr><tr><td>Ghana</td><td>96.18</td><td>1.85</td><td>33.75</td><td>0.01</td><td>2.91</td><td>0.03</td><td>0.15</td><td>001</td></tr></tbody></table>
Continued

<table><tr><td>Guatemala</td><td>85.15</td><td>1.37</td><td>33.30</td><td>001</td><td>2.69</td><td>0.05</td><td>0.08</td><td>002</td></tr><tr><td>Gueyo</td><td>102.37</td><td>1.96</td><td>34.11</td><td>0.02</td><td>2.82</td><td>0.04</td><td>0.18</td><td>003</td></tr><tr><td>Guiglo</td><td>98.95</td><td>1.73</td><td>33.90</td><td>0.07</td><td>2.77</td><td>0.03</td><td>0.14</td><td>004</td></tr><tr><td>Indonésie</td><td>96.04</td><td>1.48</td><td>34.85</td><td>0.01</td><td>2.88</td><td>0.02</td><td>0.19</td><td>001</td></tr><tr><td>Jamaïque</td><td>95.68</td><td>1.04</td><td>36.06</td><td>0.01</td><td>3.42</td><td>003</td><td>0.18</td><td>003</td></tr><tr><td>Kouibly</td><td>99.08</td><td>2.43</td><td>33.85</td><td>0.02</td><td>2.81</td><td>0.03</td><td>0.14</td><td>001</td></tr><tr><td>Madagascar</td><td>92.56</td><td>1.68</td><td>34.15</td><td>0.01</td><td>2.43</td><td>0.03</td><td>0.19</td><td>003</td></tr><tr><td>Malaisie</td><td>99.88</td><td>1.26</td><td>34.00</td><td>0.02</td><td>2.95</td><td>0.01</td><td>0.20</td><td>001</td></tr><tr><td>Man (CI)</td><td>99.45</td><td>1.40</td><td>34.81</td><td>0.03</td><td>3.13</td><td>0.05</td><td>0.17</td><td>002</td></tr><tr><td>Martinique</td><td>96.05</td><td>1.90</td><td>34.33</td><td>0.02</td><td>2.89</td><td>0.04</td><td>0.18</td><td>002</td></tr><tr><td>Oumé</td><td>94.18</td><td>1.42</td><td>33.48</td><td>0.04</td><td>2.78</td><td>0.02</td><td>0.16</td><td>003</td></tr><tr><td>Pérou</td><td>95.17</td><td>4.65</td><td>33.04</td><td>0.02</td><td>2.75</td><td>0.02</td><td>0.14</td><td>000</td></tr><tr><td>PNG</td><td>98.00</td><td>1.35</td><td>33.77</td><td>0.65</td><td>2.61</td><td>0.04</td><td>0.17</td><td>004</td></tr><tr><td>REP. Dominicaine</td><td>89.46</td><td>0.76</td><td>36.45</td><td>0.02</td><td>3.40</td><td>0.02</td><td>0.18</td><td>003</td></tr><tr><td>San Pedro</td><td>52.73</td><td>0.97</td><td>34.15</td><td>0.03</td><td>2.90</td><td>0.04</td><td>0.17</td><td>002</td></tr><tr><td>Soubré</td><td>96.80</td><td>0.98</td><td>33.26</td><td>0.02</td><td>2.78</td><td>0.04</td><td>0.18</td><td>002</td></tr><tr><td>Trinidad</td><td>97.53</td><td>1.87</td><td>33.58</td><td>0.02</td><td>2.98</td><td>0.02</td><td>0.17</td><td>003</td></tr><tr><td>Venezuela</td><td>87.85</td><td>1.38</td><td>34.72</td><td>0.03</td><td>3.05</td><td>0.04</td><td>0.21</td><td>001</td></tr><tr><td>Vietnam</td><td>101.19</td><td>1.42</td><td>33.82</td><td>0.02</td><td>2.61</td><td>0.04</td><td>0.16</td><td>003</td></tr><tr><td>Yakro</td><td>91.57</td><td>4.95</td><td>32.51</td><td>0.03</td><td>2.32</td><td>0.01</td><td>0.12</td><td>002</td></tr></table>

<table><thead><tr><th rowspan="2">Origines</th><th colspan="2">% monoinsaturés</th><th colspan="2">% des saturés (P+S)</th><th colspan="2">C-2</th><th colspan="2">C-1 et C-3</th></tr><tr><th>Mean</th><th>Standard deviation</th><th>Mean</th><th>Standard deviation</th><th>δi mean (‰)</th><th>Standard deviation</th><th>δi mean (‰)</th><th>Standard deviation</th></tr></thead><tbody><tr><td>Abengourou</td><td>31.24</td><td>0.03</td><td>66.10</td><td>0.02</td><td>-14.62</td><td>1.35</td><td>-39.55</td><td>0.67</td></tr><tr><td>Aboisso</td><td>31.57</td><td>0.03</td><td>65.54</td><td>0.03</td><td>-13.97</td><td>0.35</td><td>-39.81</td><td>0.17</td></tr><tr><td>Agboville</td><td>30.59</td><td>0.20</td><td>66.66</td><td>0.03</td><td>-15.52</td><td>0.31</td><td>-40.18</td><td>0.15</td></tr><tr><td>Alepé</td><td>30.64</td><td>0.04</td><td>66.56</td><td>0.02</td><td>-16.65</td><td>0.80</td><td>-38.85</td><td>0.40</td></tr><tr><td>Azaguié</td><td>30.90</td><td>0.02</td><td>66.55</td><td>0.01</td><td>-13.07</td><td>0.84</td><td>-38.67</td><td>0.42</td></tr><tr><td>Biankouma</td><td>31.20</td><td>0.03</td><td>66.18</td><td>0.03</td><td>-16.36</td><td>0.66</td><td>-39.19</td><td>0.33</td></tr><tr><td>Bolivie</td><td>32.11</td><td>0.03</td><td>65.40</td><td>0.02</td><td>-16.29</td><td>0.73</td><td>-40.45</td><td>0.36</td></tr><tr><td>Brésil</td><td>31.99</td><td>0.04</td><td>64.47</td><td>0.02</td><td>-14.32</td><td>1.02</td><td>-39.05</td><td>0.51</td></tr><tr><td>Cameroun</td><td>30.63</td><td>0.02</td><td>66.89</td><td>0.01</td><td>-18.19</td><td>0.41</td><td>-39.23</td><td>0.20</td></tr><tr><td>Colombie</td><td>30.58</td><td>0.03</td><td>65.91</td><td>0.01</td><td>-11.14</td><td>0.57</td><td>-39.59</td><td>0.28</td></tr><tr><td>Costa Rica</td><td>32.14</td><td>0.04</td><td>64.66</td><td>0.01</td><td>-12.76</td><td>0.70</td><td>-39.80</td><td>0.35</td></tr><tr><td>Dabou</td><td>30.51</td><td>0.03</td><td>66.46</td><td>0.02</td><td>-14.49</td><td>0.49</td><td>-40.81</td><td>0.25</td></tr></tbody></table>
Continued

<table><tr><td>Daloa</td><td>32.11</td><td>0.01</td><td>64.70</td><td>0.03</td><td>-15.70</td><td>0.89</td><td>-40.07</td><td>0.44</td></tr><tr><td>Danané</td><td>30.78</td><td>0.34</td><td>66.25</td><td>0.02</td><td>-15.25</td><td>0.69</td><td>-39.64</td><td>0.35</td></tr><tr><td>Dimbokro</td><td>30.39</td><td>0.01</td><td>67.04</td><td>0.02</td><td>-16.34</td><td>1.16</td><td>-39.18</td><td>0.58</td></tr><tr><td>Divo</td><td>30.88</td><td>0.02</td><td>66.46</td><td>0.02</td><td>-14.13</td><td>0.73</td><td>-39.91</td><td>0.36</td></tr><tr><td>Douroubé</td><td>30.77</td><td>0.03</td><td>66.51</td><td>0.03</td><td>-14.84</td><td>1.06</td><td>-40.41</td><td>0.53</td></tr><tr><td>Duékoué</td><td>30.65</td><td>0.11</td><td>66.53</td><td>0.03</td><td>-16.85</td><td>1.31</td><td>-38.09</td><td>0.66</td></tr><tr><td>Equateur</td><td>31.25</td><td>0.02</td><td>66.05</td><td>0.02</td><td>-15.39</td><td>1.00</td><td>-38.68</td><td>0.50</td></tr><tr><td>Gabon</td><td>32.82</td><td>0.03</td><td>63.78</td><td>0.03</td><td>-17.92</td><td>0.69</td><td>-38.44</td><td>0.35</td></tr><tr><td>Ghana</td><td>30.83</td><td>0.03</td><td>66.27</td><td>0.01</td><td>-14.16</td><td>0.88</td><td>-40.05</td><td>0.44</td></tr><tr><td>Guatemala</td><td>30.62</td><td>0.05</td><td>66.73</td><td>0.01</td><td>-15.77</td><td>0.75</td><td>-39.54</td><td>0.38</td></tr><tr><td>Gueyo</td><td>31.29</td><td>0.04</td><td>65.89</td><td>0.02</td><td>-15.46</td><td>1.08</td><td>-39.55</td><td>0.54</td></tr><tr><td>Guiglo</td><td>31.13</td><td>0.09</td><td>66.11</td><td>0.07</td><td>-17.22</td><td>1.38</td><td>-39.36</td><td>0.69</td></tr><tr><td>Indonésie</td><td>31.97</td><td>0.02</td><td>65.12</td><td>0.01</td><td>-15.05</td><td>0.22</td><td>-39.32</td><td>0.11</td></tr><tr><td>Jamaïque</td><td>32.64</td><td>0.03</td><td>63.87</td><td>0.01</td><td>-13.56</td><td>1.22</td><td>-37.87</td><td>0.61</td></tr><tr><td>Kouibly</td><td>31.05</td><td>0.04</td><td>66.16</td><td>0.02</td><td>-16.72</td><td>0.95</td><td>-39.77</td><td>0.47</td></tr><tr><td>Madagascar</td><td>31.72</td><td>0.03</td><td>65.85</td><td>0.01</td><td>-13.66</td><td>0.34</td><td>-38.38</td><td>0.17</td></tr><tr><td>Malaisie</td><td>31.05</td><td>0.03</td><td>66.01</td><td>0.02</td><td>-15.11</td><td>0.50</td><td>-39.59</td><td>0.25</td></tr><tr><td>Man (CI)</td><td>31.68</td><td>0.03</td><td>65.17</td><td>0.03</td><td>-13.97</td><td>0.58</td><td>-39.72</td><td>0.29</td></tr><tr><td>Martinique</td><td>31.43</td><td>0.04</td><td>65.67</td><td>0.02</td><td>-14.04</td><td>0.71</td><td>-38.80</td><td>0.36</td></tr><tr><td>Oumé</td><td>30.70</td><td>0.05</td><td>6655</td><td>005</td><td>-14.74</td><td>0.15</td><td>-38.03</td><td>0.08</td></tr><tr><td>Pérou</td><td>30.29</td><td>0.03</td><td>6700</td><td>002</td><td>-13.16</td><td>0.80</td><td>-39.47</td><td>0.40</td></tr><tr><td>PNG</td><td>31.16</td><td>0.63</td><td>6625</td><td>067</td><td>-11.59</td><td>0.82</td><td>-40.55</td><td>0.41</td></tr><tr><td>REP. Dominicaine</td><td>33.05</td><td>0.02</td><td>6346</td><td>002</td><td>-15.98</td><td>1.24</td><td>-40.66</td><td>0.62</td></tr><tr><td>San Pedro</td><td>31.25</td><td>0.02</td><td>6585</td><td>003</td><td>-18.24</td><td>0.54</td><td>-384.0</td><td>0.27</td></tr><tr><td>Soubré</td><td>30.48</td><td>0.03</td><td>6677</td><td>002</td><td>-14.55</td><td>0.64</td><td>-39.99</td><td>0.32</td></tr><tr><td>Trinidad</td><td>30.60</td><td>0.02</td><td>6644</td><td>002</td><td>-14.28</td><td>1.20</td><td>-40.75</td><td>0.60</td></tr><tr><td>Venezuela</td><td>31.67</td><td>0.04</td><td>6526</td><td>003</td><td>-14.79</td><td>0.35</td><td>-39.10</td><td>0.18</td></tr><tr><td>Vietnam</td><td>31.21</td><td>0.05</td><td>6620</td><td>002</td><td>-13.99</td><td>0.61</td><td>-39.83</td><td>0.30</td></tr><tr><td>Yakro</td><td>30.19</td><td>0.03</td><td>6756</td><td>003</td><td>-14.99</td><td>0.84</td><td>-39.53</td><td>0.42</td></tr></table>

than 4%. These results are consistent with those obtained by Anklam and Lipp (1998) [10].

Statistical processing was performed using R software [11]. The objective of this statistical treatment is to highlight the variations in the chemical composition of discriminating fatty acids on the one hand and the variations in the δ¹³C isotopic signature of glycerol on the other. Data from unsupervised univariate and multivariate analyzes will be used as appropriate. The unsupervised analysis
allows to have a global view of all the data while the supervised analysis aims to
bring out a reduced number of discriminating parameters while allowing the
distinction between two or more groups. Principal component analysis was per-
formed in order to materialize the discrimination between the samples from the
21 countries. So the samples from the Ivory Coast were all concentrated in one
value and then the difference between these different values was insignificant.
The numbers assigned to the countries in the tables represent are used in the
ACP. Thus, the principal component analysis of Figure 5 makes it possible to
highlight four (4) groups (clusters) with 81% discrimination. The parameters at
the origin of this discrimination are the chemical compositions of fatty acid li-
noleic (L10), linolenic (Ln10), palmitic (P) and unsaturated fatty acid (Oleic (O),
Linoleic and Linolenic Ln) (Figure 6). Also the PCA in Figure 7 also highlighted
(4) groups with 77.4% discrimination. The parameters responsible for this dis-
crimination are the δ¹³C isotopic signatures of glycerol (δ¹³C-2, δ¹³C-1-3) and the
chemical compositions of saturated (Palmitic and Stearic) and monounsaturated
fatty acids (Figure 8). Thus the chemical composition of fatty acids is linked to
their biosynthesis in plants [12]. As a result, the varieties of cocoa trees can in-
fluence the discrimination observed on the different PCA. In fact, linoleic fatty
acid is synthesized in abundance by gradual desaturation of oleic acid over a
short period during the development of seeds or leaves [13]. The ¹³C isotopic
deviation from glycerol is also a very important parameter in discriminating
geographic origin [14] [15]. Atmospheric CO₂ is the main source of carbon used
by plants during photosynthesis. The isotopic distribution of CO₂ is homogene-
ous in the Earth's atmosphere, however it can be incorporated in two different
ways by plants. So we have plants C3 and C4. In C3 plants, atmospheric CO₂ first
diffuses through the epidermis and stomata to the interior of the plant by a re-
versible process in which the fractionation is about 4‰. Then carboxylation by
the enzyme RuBP further discriminates the heavy isotope, with a fractionation of

<figure><img src="image_11.png" /><figcaption>**Figure 5.** Main component analysis from the chemical composition of linoleic fatty acids (L10), linolenic (Ln10), palmitic (P) and unsaturated (oleic (O), linoleic (L), linolenic (Ln)).</figcaption></figure>
<figure><img src="image_12.png" /><figcaption>**Figure 6.** The parameters used for carrying out the main component analysis: the chemical composition of the linoleic fatty acids (L10), linolenic (Ln10), palmitic (P) and unsaturated acid (oleic (O), linoleic (L), Linolenic (Ln)).</figcaption></figure>

<figure><img src="image_13.png" /><figcaption>**Figure 7.** Main component analysis from the chemical composition of saturated fatty acids (palmitic, stearic: xp.s), monounsaturated (XM) β cocoa butter and isotopic signatures δ¹³C-2 and δ¹³C-1-3 of glycerol.</figcaption></figure>

<figure><img src="image_14.png" /><figcaption>**Figure 8.** The parameters used for the realization of the main component analysis: the chemical composition of saturated fatty acids (palmitic, stearic: xp.s), monounsaturated (XM) of cocoa butter and isotopic signatures δ¹³C-2 and δ¹³C-1-3 glycerol.</figcaption></figure>
about 29‰ [16]. If carbon uptake were limited only by the carboxylation process, fractionation would be 29‰ and C3 plants would have an isotopic deviation of -37‰. At the other extreme, if carbon uptake were limited only by diffusion processes, fractionation would be 4‰ and plants would have an isotopic signature of -12‰. In reality, the total fractionation is between the fractionation due to diffusion and that due to carboxylation and the C3 plants have a between -22‰ and -30‰ [17] [18].

The isotopic composition in C4 plants differs substantially from that of C3 plants because the first carboxylating enzyme is much less discriminating for the heavy isotope [16]. After entering the plant by the same diffusion processes as for C3 plants, the CO₂ is taken over by the PEP-carboxylase enzyme. This reaction is equivalent to a 5‰ - 6‰ fractionation. The products of this reaction are then taken over by the enzyme RuBP, which slightly discriminates against the heavy isotope of carbon [16]. Thus, the sudden fractionations during the diffusion and the action of the enzymes PEP-carboxylase and RuBP determine the isotopic composition of the C4 plants, which have a δ¹³C between -10‰ and -14‰ [19].

Therefore, isotopic fractionation at the carbon level is linked to the nature of the plant.

## 4. Conclusion and Perspectives

This study made it possible to determine the chemical composition of cocoa butter in fatty acids. Thus cocoa butter is mainly composed of saturated fatty acids and then come the monounsaturated ones. We have around 63% to 68% saturated fatty acids composed mainly of stearic acid and palmitic acid, while monounsaturated fatty acids (oleic acid) represent 30% to 32%. Polyunsaturated acids represent less than 4%. We were also able to measure the δ¹³C isotopic signature of the glycerol contained in cocoa butter. The statistical treatment of these different results made it possible to highlight a discrimination within the samples. However, a study of the different varieties of cocoa could better make it possible to bring out the most discriminating parameters while eliminating the varietal factor. Also taking into account seasonal parameters (precipitation, sunshine, atmospheric conditions, etc.) could allow a more in-depth study with a view to characterizing the geographical origin of the cocoa bean.

## Conflicts of Interest

The authors declare no conflicts of interest regarding the publication of this paper.

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