# Opportunities Offered by Plant Bioactive Compounds to Improve Silage Quality, Animal Health and Product Quality for Sustainable Ruminant Production: A Review

Vincent Niderkorn, Anuraga Jayanegara

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Vincent Niderkorn, Anuraga Jayanegara. Opportunities Offered by Plant Bioactive Compounds to Improve Silage Quality, Animal Health and Product Quality for Sustainable Ruminant Production: A Review. Agronomy, 2021, 11 (1), 10.3390/agronomy11010086. [https://doi.org/10.3390/agronomy11010056](https://doi.org/10.3390/agronomy11010056)

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Review

# Opportunities Offered by Plant Bioactive Compounds to Improve Silage Quality, Animal Health and Product Quality for Sustainable Ruminant Production: A Review

Vincent Niderkorn <fnref n="1" />,* ID and Anuraga Jayanegara <fnref n="2" /> ID

**Abstract:** Plant bioactive compounds (PBC) are widespread in the plant kingdom, including in forage species, but their impact on silage fermentation and ruminant use of PBC-containing silage has been under-researched. The beneficial effects of PBC include plant-protein protection against excessive degradation by tannins or polyphenol oxidase leading to reduced soluble nitrogen (N) and better N use efficiency by animals, reduced emissions of pollutants such as enteric methane (CH₄), improved animal health through antimicrobial, anthelmintic or antioxidant activities, and positive effects on animal product quality—especially greater increased polyunsaturated fatty acid (PUFA) content. However, there are still gaps in the research that require an interdisciplinary effort to ensure a balanced approach that co-addresses the economic, environmental and health pillars of sustainability. Here we review the potential offered by PBC to improve silage quality, nutrient use efficiency, performances and health of ruminants, and product quality. In addition, we use an example of cross-fertilization between disciplines to show that incorporating PBC-containing legume species in grass silage can provide multiple and additive effects from silage fermentation to product quality.

**Keywords:** silage quality; animal health; product quality; tannins; fermentation; protein protection; methane; nitrogen; fatty acids; antioxidant

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**Citation:** Niderkorn, V.; Jayanegara, A. Opportunities Offered by Plant Bioactive Compounds to Improve Silage Quality, Animal Health and Product Quality for Sustainable Ruminant Production: A Review. *Agronomy* **2021**, *11*, 86. https://doi.org/10.3390/agronomy11010086

Received: 17 November 2020

Accepted: 23 December 2020

Published: 5 January 2021

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**Copyright:** © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

## 1. Introduction

Several approaches have been proposed in an effort to improve the sustainability of animal production systems by adapting agroecological principles [1]: reducing inputs needed for production, reducing pollution, integrating the management of animal health, exploiting system diversity and preserving biological diversity [2]. Applying these general principles and approaches to resource use for ruminant nutrition creates a number of challenges. There is a need to increase nutrient use efficiency by the animals while decreasing nitrogen (N) excretion and methane (CH₄) emissions at the animal level [3], to reduce livestock exposure to pathogens, toxins and oxidative stress [4], and to improve animal product quality, for instance through better meat fatty acid (FA) profiles or oxidative stability [5].

All these challenges come into play for silages, which are an important source of forage outside the growing season in many countries. The demand for high-quality silage to provide forage for ruminants is also made more acute by heightening food-feed competition over limited arable land resources, especially for monogastric livestock. The issue of energy and protein losses (with concomitant pollutant emissions) is even more crucial, as they begin with enzymatic and microbial processes in the silos, long before actual intake of the silage. These losses come in the form of fermentation gases and

<footnote n="1">¹ INRAE, VetAgro Sup, UMR Herbivores, Université Clermont Auvergne, 63122 Saint-Genès-Champanelle, France</footnote>

<footnote n="2">² Department of Nutrition and Feed Technology, Faculty of Animal Science, IPB University, Bogor 16680, Indonesia; anuraga.jayanegara@gmail.com</footnote>

<footnote>* Correspondence: vincent.niderkorn@inrae.fr</footnote>
juices that contain various amounts of energy and soluble N depending on harvest-plant and fermentative process factors [6]. Moreover, silage quality strongly affects silage use by the animals, especially as previously partly-degraded protein will be more degraded in the rumen and thus more N will get excreted as urea [7,8]. There are also potential health issues specific to silage due to the presence of pathogens or mycotoxins produced during the silage-making process [9,10] that can have impacts on ruminant health and performances [11,12] or lead to residues in animal products [13].

Plant bioactive compounds (PBC) have long been recognized as a fertile source of new drugs in the pharmaceutical industry. Mounting public concern over the use of chemicals and pharmaceuticals in ruminant production systems has driven interest in PBC as alternative rumen modifiers and performance enhancers [14]. The biological activity of PBC depends mainly on their chemical nature and concentration in animal diets, with contrasted effects on animal responses. The main desired effects are those promoting animal health (antimicrobial, anthelmintic, anti-bloating, antioxidant, immune stimulator) and performance (production, N use efficiency) and product quality [15], although some PBC (including the beneficial ones used at an undesirable concentration) can be anti-nutritional or even toxic to animals. There has been extensive investigation into the use of PBC in ruminant nutrition, but the value of PBC in silage remains under-researched.

Here we review the state of the art on PBC use in silage as part of practices to promote more sustainable ruminant production systems. Our scope includes opportunities opened up by PBC to improve: (i) silage lactic fermentation and plant protein protection to increase nutrient use efficiency and decrease pollutant losses in the form of greenhouse gases and ammonia ($NH_3$), (ii) animal health (e.g., digestive diseases), and (iii) product quality, for example through FA profiles in meat and milk or increased oxidative stability. We also report results from the EU-funded Marie Curie project LegumePlus (FP7-PEOPLE-2011-ITN-289377, 2012–2015) in which these different aspects were covered together. One objective of the LegumePlus project was to check whether different pillars of sustainability (productivity, environment and product quality) can be co-achieved using two PBC-containing forage legumes, i.e., sainfoin (containing condensed tannins) and red clover (containing polyphenol oxidase; PPO) included in grass-based silage. Their potential benefits and associative effects on silage quality, voluntary intake, digestion, animal performances and meat quality were measured in sheep [16]. The results of this work are reported throughout the manuscript and summarized in Figure 1. The paper concludes by identifying gaps in the research needed to integrate PBC-enhanced silage into farming practices.

## 2. Classes of Plant Bioactive Compounds and Silages

The plant kingdom features a hugely diverse array of bioactive compounds, many of which are found in forage whole plants, feeds or plant extracts that can be incorporated into silage. PBC can be found in grassland plants, crops, agroindustry byproducts, and plant extracts or essential oils in which PBC have all been characterized to varying degrees.

Despite growing interest in the use of PBC in animal nutrition, their use in silages remains limited. Table 1 recaps the results of a literature search on specific classes of plant compounds. Plant molecules were searched based on chemical structure (lipid, aromatic, alkaloid, polyphenol, tannin, saponin, terpene) in the Web of Science, and each class was refined using the terms “bioactive or antimicrobial or anthelmintic or antibiotic”, “silage”, or both. The results highlight the contrast between the large number of articles reporting bioactivity for these compounds and the little literature focused on their effects in silage. Reasons that may explain this substantive lack of research are the low value of silage, the need for farmers to make silage as easily as possible, and other factors known to improve silage quality (ensiling techniques, inoculants). However, although difficult to assess, the economic impact of poor-quality silage, animal disease and reduced performances can be significant. Some bioactive compounds naturally present in a number of forage species or byproducts are potentially cheap yet could bring complementary benefits to existing
solutions. The small amount of literature available on PBC bioactivity in silage tends to focus on polyphenols, and especially tannins.

<table><caption>**Table 1.** Number of references associated to plant bioactive compounds and silage.</caption><thead><tr><th rowspan="2">Compound Class</th><th colspan="4">Number of References <sup>1</sup></th></tr><tr><th>Total</th><th>Bioactive</th><th>Silage</th><th>Bioactive × Silage</th></tr></thead><tbody><tr><td>Lipid</td><td>650,783</td><td>22,544</td><td>676</td><td>10</td></tr><tr><td>Aromatic</td><td>306,292</td><td>1616</td><td>24</td><td>2</td></tr><tr><td>Alkaloid</td><td>88,000</td><td>6603</td><td>52</td><td>3</td></tr><tr><td>Polyphenol</td><td>63,497</td><td>8476</td><td>128</td><td>11</td></tr><tr><td>Tannin</td><td>24,994</td><td>3248</td><td>332</td><td>18</td></tr><tr><td>Saponin</td><td>24,299</td><td>3269</td><td>39</td><td>2</td></tr><tr><td>Terpene</td><td>15,830</td><td>1412</td><td>21</td><td>2</td></tr></tbody></table>

### 3. Improving Silage Quality

Silage quality is highly dependent on the capacity to maintain the integrity of plants at harvest. Plant degradation and decrease in nutritive value start with the post-harvest continuation of plant respiration leading to hydrolysis of carbohydrates into CO₂ and consumption of sugars, which are essential substrates for lactic fermentation [17]. Consequently, the delay between harvest and ensiling should be reduced as much as possible. During silage fermentation, the proteins, which typically represent 70–80% of the total N of the plant, are partly degraded by plant proteases to soluble N and especially amino acids and NH₃. Proteolysis raises the soluble N content to 40–60% of total N and continues until the pH drops below 4.0 [17]. The usual way to reach anaerobiosis and the desired pH as rapidly as possible is through an optimal management of silage: correct dry matter concentration at harvest, rapid silo filling, perfect mass sealing, high mass compaction [6]. Unappropriated management procedures and unloading technique, or the use of machinery not suitable to ensure sufficient compaction can lead to air penetration and consequent aerobic deterioration of the ensiled mass. The use of inoculants based on homo- and/or hetero-fermentative lactic acid bacteria can offer a significant help in some situations [18]. Chemical additives can provide a more consistent effect than inoculants because they are less dependent on biological processes, but their higher cost or toxicity may be a limit to adoption by farmers. For instance, benzoate or sorbate have been shown to be safe and of high effectiveness against molds and yeasts, while formaldehyde could be toxic due to irritation of the skin, eye and respiratory organs [19] (for a detailed review on the silage additives, see [18]).

PBCs offer a complementary option to these solutions as they can be used at negligible cost when incorporated in the form of bioactive forages or agroindustry byproducts. Research efforts have focused more on the ability of PBC to reduce protein degradation in the silo, and less on their ability to improve lactic fermentation. A recent meta-analysis unequivocally showed that tannins effectively and efficiently improve silage quality, especially by limiting the extensive proteolysis that may occur during ensiling [20]. Tannin-rich forage legumes are also packed with protein, which make them particularly good candidates to include in silage. Ensiling temperate tannin-rich legumes such as sainfoin or sulla can protect plant proteins during fermentation, reduce NH₃ production, and improve nutritive value compared to tannin-free forages [21–23]. Tannins have also been shown to limit proteolysis in high-protein silage such as alfalfa, moringa, indigofera and other legume silages [20,24,25]. In a study comparing the effects of tannin levels in sorghum silages supplemented or not with tannin-inactivating polyethylene glycol (PEG), silages without PEG had higher protein levels and lower NH₃ concentrations than silages with PEG [26]. Tannins can also be successfully supplemented in silage via byproducts (e.g.,

<footnote n="1">¹ Searched on 21/04/2020 using Web of Science. Each compound class was first searched as an independent topic then refined using: "Bioactive or antimicrobial or anthelmintic or antibiotic", "silage", and both.</footnote>
green tea waste, [27] and plant extracts containing hydrolysable tannins from chestnut and oak [28,29] or condensed tannins from quebracho [30].

PPO is another bioactive compound found in forages, especially red clover. PPO is an enzyme that can catalyze the oxidation of phenols to quinones, which are highly reactive protein-binding molecules, and thus provide effective protection against proteolysis and lipolysis [31]. PPO is particularly relevant for silages because PPO action requires (i) cellular damages in order to obtain contact between PPO located in chloroplasts and phenols located in vacuoles, and these damages are obtained during harvest, wilting and silage-making processes, and (ii) a time window of aerobiosis for oxidation, which occurs before the stabilization of the silo but not at grazing where the period of aerobiosis between chewing the forage and entry into the rumen is not enough long [32].

Mixing a grass (timothy) with sainfoin and/or red clover in micro-scale silos has been demonstrated to improve fermentation and protein protection against degradation through lower pH values, decreased soluble N and NH₃ contents, and higher concentrations of lactic acid compared to pure ensiled grass (Figure 1A–D), thus confirming the benefits of condensed tannins and PPO for silage quality [33].

Other PBC including erythritol byproduct solution and lignosulphonates from wood molasses were studied as silage additives to reduce in-silo proteolysis but were shown to be less efficient compared to tannin solutions [29]. In contrast, spraying essential oils (eugenol, thymol, cinnamaldehyde and carvacrol) before ensiling ryegrass was shown to reduce protein degradation, but usually had to be sprayed at a concentration of 2 g/kg fresh forage to observe inhibition of amino acid deamination [34]. Finally, without really being considered as PBC, sugars added at ensiling or present in high-sugar grasses are well known to facilitate lactic fermentation, especially when the sugar content of forage is a limiting factor [35]. It should be noted that sugars can also be a substrate source for undesirable microorganisms if other inhibitory factors are missing or the silo management is inadequate. In particular, the saccharolytic *Clostridia* are able to ferment sugars to butyric acid, and excessive concentration of sugars can decrease aerobic stability, as residual sugars are rapidly used by spoilage yeasts and molds [18].

<figure><img src="image_4.png" /><figcaption>Figure 1. *Cont.*</figcaption></figure>
<figure><img src="image_5.png" /><figcaption>**Figure 1.** (A) pH values, (B) lactic acid concentration, and proportions of (C) soluble nitrogen (N) and of (D) ammonia (NH₃) in total N in microscale silos of pure timothy (T, black bars), pure sainfoin (SF, grey bars), pure red clover (RC, white bars), binary mixture T-SF (50:50%), ternary mixture T-SF-RC (50:25:25%), and binary mixture T-RC (50:50%) (n = 3 for each type of silage) [33]. (E) Organic matter (OM) digestibility, (F) methane (CH₄) yield, (G) urinary and (H) faecal N in sheep fed silages of T, T-SF, T-SF-RC, T-RC and SF-RC (binary mixtures: 50:50%, ternary mixture: 50:25:25%) (n = 10 sheep allocated in a replicated Latin square design) [36]. (I) Voluntary intake, (J) kinetics of live weigh, (K) polyunsaturated fatty acids (PUFA) in rumen content and (L) ratio of n-6:n-3 fatty acids (FA) in *Longissimus* muscle of growing lambs (n = 8 lambs per group) fed the same silages as in (E–H) [37,38]. a,b,c For each sub-figure, values with different letters significantly differ (*p* < 0.05).</figcaption></figure>

4. Increasing Nutrient Use Efficiency and Animal Performances in Ruminants

Beyond improving silage quality, PBC—and particularly tannins—may have the potential to enhance nutrient (N and energy) use efficiency in ruminants and, in turn, animal performances. However, it remains to be seen whether the PBC provide further biological effects once ingested by ruminants. The question is a relevant one, as the hydroxyl groups in the structure of tannins are already used to bind protein in the silage, which may leave insufficient hydroxyl groups to deliver any further effects in the rumen. However, PBC in silage can still improve N use efficiency only when specific bioactives also protect against excessive microbial protein degradation in the rumen, which means they can simultaneously reduce rumen-degradable protein and increase rumen-undegradable protein. Increased rumen-undegradable protein, which is often called rumen bypass protein, leads to an increase in metabolizable protein, i.e., protein that can be digested and absorbed in the small intestine. A key indicator of protein degradation in the rumen is NH₃. Although rumen microbes require a certain amount of to synthesize microbial protein, NH₃ overage in the rumen is a form of inefficiency of N use and can subsequently stimulate higher N excretion to the environment.

Various experimental results have shown that tannins in silage could modify rumen fermentation and proteolysis. For instance, increasing supplementation level of *Flemingia macrophylla* silage, a tropical shrub legume containing condensed tannins, reduced in vitro ruminal NH₃ concentration in both high-roughage and low-roughage diets [39]. Apart from being natively present in the material ensiled, tannins can also be added to silage to modulate fermentation processes. Adding tannin extract from chestnut (on a 2% and 4% dry matter basis) to moringa and indigofera legume silages decreased NH₃ concentration
both in the respective silages and during in vitro rumen fermentation [20]. In agreement with in vitro results, an in vivo experiment in beef cattle showed that adding tannic acid (a hydrolysable tannin) to an alfalfa silage-based diet reduced ruminal NH₃ concentration by 18.1% more than control [24].

A decrease of excessive proteolysis during rumen fermentation when PPO-containing red clover is ensiled together with other forage species has been evidenced in vitro both with temperate [40] and tropical grasses [41]. Note, however, that as PPO is inactive in the anaerobic conditions of the rumen, the protection of plant protein can only be the result of pre-ingestive events [42]. In vivo, a significant reduction of rumen NH₃ release per unit of dietary N consumed is observed for red clover-containing silages compared with other forages [43,44], reflected by a higher flow of non-NH₃-N to the small intestine corresponding to undegraded or partially degraded dietary protein. This results in an increase in N use efficiency when dietary N intake is balanced. However, when surplus dietary N is not balanced with more fermentable energy to maximize microbial protein synthesis in the rumen, urinary N losses increase [42].

The enhanced silage quality when sainfoin and red clover were included in grass silage was reflected in rumen digestion as shown in an in vitro rumen fermentation assay in which several parameters were positively affected [45]. In particular, ensiling red clover or red clover-sainfoin mixture with timothy has been shown to reduce the ratio of NH₃ to insoluble N (as an indicator of rumen proteolysis) by 25.2% and 28.9%, respectively, compared to controls. A positive associative effect between timothy and red clover on the production of volatile fatty acids (VFA) was also observed. The effects of timothy silages with vs. without sainfoin, red clover or both on total tract digestion were then investigated on sheep in metabolic cages [36]. Results showed that N excretion was directed towards feces rather than urine with the timothy-sainfoin mixture (Figure 1G,H). This finding suggests that the tannin-protein complexes did not completely dissociate during their transit through the digestive tract, or may confirm the hypothesis that the complexes can re-form under the alkaline conditions of the intestine after dissociation in the acidic conditions of the abomasum [46]. This 'shift' in N excretion from urine to feces is environmentally beneficial, because urinary N is more volatile and therefore more quickly converted into greenhouse gases (nitrous oxide) than fecal N [47]. However, it did not yield any productive benefit, as the proportion of N retained in animal tissues was not significantly improved. N digestibility was greater for pure grass and the timothy-red mixture clover than for the timothy-sainfoin mixture, and the amount of N retained daily by the animals was greater for red clover-containing silages than for other silages [36].

Turning to energy use efficiency, PBC have been shown to mitigate enteric CH₄ emissions from ruminants [48]. Apart from its contribution to global warming, enteric CH₄ emission *per se* is a form of animal energy loss. Approximately 4–14% of ruminant gross energy intake is lost as CH₄, so mitigating CH₄ emissions also contributes to more efficient energy utilization by ruminants. Increasing the proportion of red clover silage to orchardgrass linearly reduced CH₄ emissions per unit of dry matter intake in sheep [49]. Increasing the level of *Flemingia macrophylla* silage supplementation in rice straw-based diets decreased in vitro CH₄ emissions and was accompanied by a decrease in protozoa population [39]. The use of this tannin-containing local fodder shrub in silage has also been shown to improve nutrient digestibility, rumen fermentation efficiency, and milk production in lactating dairy cows [50]. In vitro, adding chestnut tannin extract into moringa and indigofera legume silages reduced CH₄ emissions [20]. In vivo CH₄ emissions were lower in beef cattle consuming an alfalfa silage-based diet treated by gallic acid, a subunit of hydrolysable tannins [24]. The ability of tannins to mitigate enteric CH₄ emissions is possible via a number of mechanisms. Tannins inhibit microbial degradation of plant cell wall such as cellulose and hemicellulose, and also reduce the formation of hydrogen, which is a major substrate for methanogenesis. PBC also inhibit methanogens [51], the microbial group responsible for methanogenesis in the rumen, and reduce populations of protozoa [52] that live in symbiosis with methanogens. Note that the tannin-induced reduc-
tion in CH4 emissions is often accompanied by a reduction of organic matter digestibility, as shown in metabolic cages with the inclusion of sainfoin in silage compared to pure timothy (Figure 1E,F; [36]).

PBC-containing silage can enhance the productive performance of ruminants via improved N and energy use efficiency, provided that the bioactive compounds are present in moderate concentrations, i.e., not too low and not too high. The effect of PBC in silage on nutrient use efficiency and animal performance is not, therefore, linear. For instance, increasing proportion of red clover silage to orchardgrass silage quadratically decreased urinary N loss and tended to quadratically increase N retention in sheep. Red clover silage at a 25% feed inclusion level had the highest N retention, whereas higher levels led to a decline in N retention [49]. Dietary tannins have to be at a moderate level to generate optimal N use efficiency and animal performance [46,53]. Negligible or very low dietary tannin levels would not affect the performance, while overly-high dietary tannins (typically > 5% dry matter) would reduce rumen fermentation, digestion parameters and animal performance. A precondition for successful use of dietary tannins is that the compounds bind and protect protein from microbial degradation in the rumen by forming tannin-protein complexes, but the complex needs to be released at low pH in the abomasum to make the released protein available for further digestion and absorption in the small intestine. At too high dietary tannin concentrations, tannin-to-protein binding is very strong and hard to release in the abomasum, making the protein unavailable in the small intestine. A study comparing animal performances in growing lambs fed different silage mixtures containing or not sainfoin and red clover (or both) found strong differences among PBC-containing forages [37]. Lambs fed mixtures containing red clover showed higher voluntary dry matter intake compared to lambs fed pure timothy or the timothy-sainfoin mixture (Figure 1I), and lambs fed silage containing 50% red clover had 29.8% average daily gain than lambs fed pure timothy (Figure 1J). Logically, this led to higher growth rates, reaching up to 235 g/day for the timothy-red clover mixture (against 145 g/day for the timothy-sainfoin mixture), and to carcass weights of up to 20.5 kg for the timothy-red clover mixture (against 17.2 kg for the timothy-sainfoin mixture).

**5. Maintaining or Improving Ruminant Health**

The ensiling process is managed on-farm by farmers who do not have the same kind of quality control resources as manufacturers of marketed feeds. The compliance of good practices in silo management remains the best way to limit the risk that silages contain undesirables affecting the animal. Silage-related hazards to ruminant health are mainly due to undesirable micro-organisms competing for nutrients with lactic acid bacteria or using lactate during the fermentation (Enterobacteria, Clostridia), aerobic spoilage due to yeasts and molds, mycotoxins, and metabolic disorders [54]. There are very few papers reporting the effects of PBC on ruminant health specifically in silage. However, PBC that have been shown to be active in non-silage diets can also be active when incorporated in silage, as long as they do not get removed or biotransformed by microorganisms and enzymes during ensiling.

Mycotoxins in silage pose a major risk for ruminant health [10]. The main toxic effects and risks are reduced feed intake and production, reproductive problems, immunosuppression, and there is also a non-negligible risk of certain metabolites (e.g., aflatoxin M1 or ochratoxin A) transferring into meat and milk and potentially causing problems for human health. To our knowledge, in contrast with other additives such as chemicals and inoculants [11], PBC have never been investigated or shown as a possible solution against the production of mycotoxins in silage. With the exclusion of Penicillium species, typical silage molds (Fusarium, Aspergillus, Monascus, Rhizopus, Geotrichum) are intolerant to low-oxygen. Thus, the adoption of correct ensiling procedures enabling to reduce the area exposed to risk of air penetration, such as appropriate dry matter content and particle sizes at ensiling or optimal mass compression, remains the best strategy to limit the presence of molds and mycotoxins in silage. It should be noted that, although high concentration of
sugars in ensiled material helps stimulate fermentation, it tends to aerobic instability, as a lot of sugar may still be available for aerobic microorganisms [18].

Parasitism with gastrointestinal nematodes remains ubiquitous in grazing ruminants, and on-farm control largely depends on regular use of anthelmintic drugs, resulting in increased nematode resistance and reduced drug efficacy worldwide [55]. Thus, the identification of complementary or alternative solutions such as cures of PBC with anthelmintic activity is crucial to achieve sustainable parasite control in ruminants. Several forage plants have been shown to have anti-parasitic activity, including when used as silage. Chicory silage containing sesquiterpene lactones significantly reduced *Ostertagia ostertagi* adult burdens in cattle [56]. *Sericea lespedeza* and sainfoin silages containing condensed tannins demonstrated anthelmintic activity in vitro by inhibiting the exsheathment of *Haemonchus contortus* larvae, which is a crucial step in the life-cycle of parasites since it represents the transition from the free-living to the parasitic stages, and in vivo by decreasing adult worm burden and fecal egg counts in small ruminants [57,58].

There is evidence in farm animals of a detrimental effect of inflammation and oxidative stress characterized by imbalance between the formation of oxidants (radicals and non-radicals) and their detoxification by the antioxidant system, causing compromised immune responses and health disorders [4]. Although many of the antioxidant defense capacities of ruminants are derived from vitamins and minerals [59], supplying PBC in ruminant's diet appears to be a promising approach. The main PBC reported to limit inflammation and oxidative processes are polyphenols, but the modes of action are still not fully elucidated [60]. It was shown that grape polyphenols may induce cytoprotective mechanisms after calving to cope with oxidative and inflammatory stress [61]. An additional study also indicated a potential reduction in endoplasmic reticulum stress and metabolic stress in the liver by the plant polyphenols [62]. Another effect of plant antioxidants is that they help prevent lipid oxidation, especially for polyunsaturated fatty acids (PUFA) that are particularly susceptible to oxidative damage, resulting in lipid peroxidation of membrane phospholipids that can affect normal cellular functions [63–66]. The PBC with an antioxidant potential can be supplied through silage feeding, and studies usually show far higher concentrations in herbaceous species than in corn [67], with variations according to the botanical families, seasons, and production systems [68].

## 6. Effects on Product Quality

An additional beneficial effect of PBC with antioxidant potential as described above is an increase of PUFA content and oxidative stability in ruminant products, allowing to produce animal-source foods with health-promoting properties for human. This attribute has been partly assigned to PUFA, particularly n-3 FA [69] and conjugated linoleic acid (CLA; [70]), but not assigned to saturated FA (SFA). Unfortunately, large amounts of PUFA undergo transformation processes in the rumen through microbial biohydrogenation to form FA with a higher degree of saturation [71]. This explains the high SFA content in ruminant products and the potential health risks tied to their consumption. The biohydrogenation process therefore needs to be controlled in order to obtain better FA profiles in farmed animal products, which makes it vital to develop nutritional strategies for optimizing the process while maintaining optimal rumen function and fermentation.

Increasing the PUFA content, especially omega-3 FA, in ruminant products is a desirable way forward, as unlike SFA, omega-3 FA are thought to bring health benefits such as reducing the risk of cardiovascular disease and lowering plasma cholesterol levels [69]. Promoting the level of c9,t11 C18:2, which is produced from partial biohydrogenation of C18:2 n-6 in the rumen, is of interest since it has been shown to prevent cancer proliferation, decrease atherosclerosis, and improve immune response [72]. In relation to the respective CLA isomer, t11 C18:1 is also desirable since it can be converted in ruminant tissues of to c9,t11 C18:2 via the action of the enzyme Δ-9 desaturase (stearoyl-CoA desaturase) by adding a cis9-double bond [71]. Any nutritional measure for manipulating the biohydrogenation of FA in the rumen is therefore directed towards these objectives. Feeding forages
to ruminants is associated with improved FA profiles in the products, since lipids in forages tend to be naturally rich in PUFA [73]. For instance, it has been shown that feeding grass-based diets results in higher levels of C18:3 n-3 in muscle compared to concentrate-based diets [74]. Another study observed that feeding grass silage to fattening bulls led to higher C18:3 n-3 in the adipose tissue compared to bulls fed maize silage [75]. The positive effect of dietary grass supplementation on the fatty acid profile of milk and derived products (e.g., cheese) has also been demonstrated in different lactating species such as cows [76], buffalo [77] and ewes [78]. Forage species, cultivar, conservation method and level of inclusion are all sources of variation in the rates and extents of ruminal biohydrogenation of dietary FA from forages as well as their transfer to products [79].

Tannin has been reported to reduce PUFA biohydrogenation, accumulate t11 C18:1 and/or decrease C18:0 concentration [80–84], and to increase the rate of C18:3 n-3 transfer from feed to milk [85], and may thus alter FA composition in the animal products [74]. In vitro incubations of tropical [83] and alpine plants [84] with added linseed oil suggest the ability of plant phenolics to modulate FA biohydrogenation, i.e., by decelerating the process right from the first step. This was indicated by lower disappearance of C18:3 n-3 and C18:2 n-6 in the incubations of both tropical and alpine plants containing high phenolics. Such effects may be explained by the toxicity of phenolics, particularly tannin phenolics, to bacterial species involved in FA biohydrogenation [80,86]. It is also possible that phenolics inhibit the process of lipolysis, which is a prerequisite to further transformation of FA, i.e., biohydrogenation [87,88]. In line with this argument, research has found a negative relationship between tannins present in *Vicia sativa* and *Trifolium incarnatum* and the biohydrogenation of C18:3 n-3 [82]. Other phenolics are thought to drive the increase in C18:3 n-3 transfer from feed to milk in animals fed buckwheat [85], phenolics which might be rutin or fagopyrin [89].

Regarding the influence of plant phenolics on the occurrence of biohydrogenation intermediates, an in vitro study [80] found a considerable increase of t11 C18:1 to the expense of C18:0 when adding *Acacia mearnsii* extract (source of condensed tannins) at 79 g/kg dry matter to a mixed grass-clover hay diet supplemented with linseed oil. The authors suggested that the tannins inhibited the terminal step of FA biohydrogenation. The accumulation of t11 C18:1 in the presence of tannin phenolics was confirmed by another in vitro study [81]. This effect might be related to the toxicity of phenolics, especially tannin, to bacterial species involved in FA biohydrogenation, through selective inhibition of cell wall synthesis [90], interaction of phenols with microbial proteins [91], and direct phenol-lipid interactions [92]. *Clostridium proteoclasticum*, a bacterial species responsible for the terminal step of biohydrogenation, i.e., the conversion from t11 C18:1 to C18:0 [93], was proved to decrease by 31% in the rumen of lambs fed a tannin-supplemented diet compared to a control diet [86].

The different structures of tannins, i.e., hydrolysable and condensed tannins, may serve to modify the biohydrogenation pattern. Hydrolysable tannins were the main class of tannins that prevented C18:3 n-3 and C18:2 n-6 from biohydrogenation, whereas condensed tannins were more closely correlated with the appearance of c9,t11 C18:2 [83]. This illustrates that both types of tannins are involved in the inhibition of biohydrogenation, but in different steps, i.e., hydrolysable tannins in the first step, and condensed tannins in the second step. By contrast, it has been observed in vitro and in vivo that adding condensed tannin extracts to diet led no difference in concentration of conjugated C18:2 in the ruminal fluid but instead led to a considerable increase of t11 C18:1 to the expense of C18:0 [80]. This indicates that inhibition of the third step of biohydrogenation took place. Different tannins thus have a differentiated and not-always-coherent influence on the biohydrogenation pathway.

Note that the effect of tannins on FA biohydrogenation cannot be found for all the dietary resources [94], and it appears that phenolics have only minor effects when the concentration is below a certain threshold [83,95]. For instance, supplementation of commercially-available tannin extracts, i.e., a 1:1 mixture of *Castanea sativa* and *Schinopsis*
*lorentzii* (quebracho, a source of condensed tannins) at a level of 10 mg/g dry matter to a diet containing sunflower oil did not alter the proportions of the major FA classes in milk, i.e., PUFA, mono-unsaturated FA (MUFA) and SFA, nor the proportions of c9,t11 C18:2 and t11 C18:1 [96]. The low dose of the tannin mixture in that study was presumed to have been the reason for the lack of change in FA found. It appears that both the levels of dietary polyphenols and the sources from which they were derived are among the factors affecting ruminal FA biohydrogenation and general rumen fermentation [83].

Tannins in silages continue to demonstrate effects on biohydrogenation. Adding oak tannin extract at 26 g/kg dry matter to grass silage increased unsaturated FA content and reduced SFA content in the milk of lactating dairy cows [97]. The effect of PPO in red clover included in silage has also been confirmed [31]. The exact mode of action of PPO protecting PUFA against biohydrogenation in the rumen is not fully elucidated, though several potential mechanisms have been evoked: quinone binding to polar lipids, reduction of microbial lipase activity following the formation of protein complexes around glycerol-based lipid, or changes in rumen microbial populations [42].

The partial or total substitution of timothy with sainfoin or red clover was associated with greater concentrations of PUFA and lower concentrations of MUFA in the rumen content (Figure 1K, [38]). In particular, the inclusion of these two bioactive legumes in silage favoured the accumulation of 18:3 n-3, with the greatest concentrations being observed for the sainfoin-red clover mixture. The dietary treatment also tended to affect the proportions of MUFA and PUFA in the intramuscular fat of lamb longissimus muscle. Sainfoin and red clover clearly had an additive effect on reducing omega-6/omega-3 FA ratio (Figure 1L), which is a desirable way to also reduce the risk of many chronic diseases. Therefore, this study indicated that the beneficial effects of bioactive PPO and tannins on rumen biohydrogenation and meat quality appear to be similar and additive. It was also shown that feeding lambs with silages containing sainfoin and red clover can improve the oxidative stability of the meat, as evidenced when meat was subjected to strong oxidative challenge, such as cooking and incubation with pro-oxidant catalysts [98]. This is particularly beneficial as meat with high PUFA and poor oxidative stability results in deterioration of its flavor, odor and color [64], and lipid oxidation end-products may facility carcinogenic processes in the colon [65].

## 7. Conclusions and Directions for Future Research

Silage quality can impact all the dimensions of ruminant nutrition, from nutritive value to palatability and voluntary intake and on to animal health and product quality. This greatly impacts the inputs needed for animal performances (energy and protein concentrates, drugs, and so on) as well as the pollutant emissions that need to be minimized as far as possible to support a more sustainable model of agriculture and agroecology. The use of PBC emerges as a natural solution, relatively cheap especially if PBC are supplied by ensiled forages, and well accepted by consumers. In this review, we have highlighted the ability of PBS, especially tannins and PPO contained in some legumes or in plant extracts, to reduce degradation of dietary protein into soluble N and NH₃ in the silo and in the rumen. This can lead, but not always, to increased nutrient use efficiency and animal performances, and decreased pollutions under the form of urinary N excretion and CH₄ emissions. Polyphenolic compounds can have beneficial effects on ruminant health, especially through the anthelmintic activity of tannins and their antioxidant capacity to cope with inflammatory and oxidative stress. Finally, some PBS have been shown to reduce FA biohydrogenation resulting in increased PUFA content and oxidative stability in ruminant products. However, despite major strides forward in recent years on the effects of PBC in animal nutrition, questions remain as to how to translate research results into practices:

What agronomic, harvesting, or ensiling measures can ensure that PBC deliver consistent results, taking into account the diversity of PBC and expected effects?
How do the animals—and especially their rumen ecosystem—adapt to PBC? What are the consequences for long-term PBC efficiency?

How can we develop tools for improving PBC analysis in silage and digesta to better understand and predict PBC effects in the silo and in the animal?

How can we modulate the stability of the tannin-protein complexes throughout the digestive tract to allow a greater intestinal N absorption and improved N use efficiency?

When PBCs have multiple effects, is there a competition between these effects? For instance, are tannins already engaged in protein binding in the silo are still available to act on methane emissions or parasites?

How can we better integrate the researches on the antioxidant capacity of some PBC, encompassing effects on animal health and human health-promoting animal products?

Do PBCs hold equivalent potential in temperate and tropical areas, given that local PBC contents and profiles are substantially different? Which does this mean for use in practice?

Interdisciplinary research and cross-fertilization of disciplines are both key to co-
addressing the different pillars of sustainability. Practical solutions need to be developed
through collaboration between chemists, ruminant nutritionists, farmers, veterinarians,
producers and consumers, to deliver sustainable innovations and produce high-quality
products profitably while also maintaining the quality of the environment.

**Author Contributions:** Bibliographic review, V.N. and A.J.; writing—review and editing V.N. and A.J.; supervision, V.N. All authors have read and agreed to the published version of the manuscript.

**Funding:** This research received no external funding.

**Conflicts of Interest:** The authors declare no conflict of interest.

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