# Draft genome sequence of the candida zemplinina (syn., Starmerella bacillaris) type strain CBS 9494

Alberto Luis Rosa, Cecile Miot-Sertier, Yec’Han Laizet, Franck Salin, Matthias Sipiczki, Marina Bely, Isabelle Masneuf-Pomarede, Warren Albertin

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Alberto Luis Rosa, Cecile Miot-Sertier, Yec’Han Laizet, Franck Salin, Matthias Sipiczki, et al.. Draft genome sequence of the candida zemplinina (syn., Starmerella bacillaris) type strain CBS 9494. Microbiology Resource Announcements, 2018, 7 (10), pp.1-4. 10.1128/MRA.00872-18. hal-02620026

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Distributed under a Creative Commons Attribution 4.0 International License
# Draft Genome Sequence of the *Starmerella bacillaris* (syn., *Candida zemplinina*) Type Strain CBS 9494

Alberto Luis Rosa,ª Cécile Miot-Sertier,ᵇ Yec'han Laizet,ᶜ Franck Salin,ᶜ Matthias Sipiczki,ᵈ Marina Bely,ᵇ Isabelle Masneuf-Pomarede,ᵇ,e Warren Albertinᵇ,f

**ABSTRACT** *Starmerella bacillaris* is an ascomycetous yeast ubiquitously present in grapes and fermenting grape musts. In this report, we present the draft genome sequence of the *S. bacillaris* type strain CBS 9494, isolated from sweet botrytized wines, which will contribute to the study of this genetically heterogeneous wine yeast species.

Starmerella bacillaris (syn., Candida zemplinina) (1) is a non-Saccharomyces yeast ubiquitously present in oenological environments (2–7) and occasionally recognized in soil, fruit insects, and some rotten fruits (5, 8, 9). The species, first recognized in Napa Valley (USA) in Botrytis-affected wine fermentations (10), was then identified through a detailed morphological, physiological, and molecular characterization of the *S. bacillaris* type strain 10-372 (=CBS 9494T =NCAIM Y016667T), isolated from white wine in Zemplin, Hungary (11). The potential use of *S. bacillaris* in winemaking has been studied in mixed fermentations with Saccharomyces cerevisiae, where its fructophilic character and ability to produce wines with reduced ethanol levels represent potential advantages (12–19). Sensory evaluation revealed that pure starters of *S. cerevisiae* were preferred to mixed *S. cerevisiae/S. bacillaris* fermentations (20). Phylogenetic analyses of *S. bacillaris* strains isolated from winemaking environments showed that neither clonal-like behavior nor specific genetic signatures were associated with strain populations at the different analyzed vineyards and wineries (21). These studies suggest that *S. bacillaris* is not under selective pressure in winemaking environments since its genetic diversity is shaped by geographical localization (21). The availability of the genome sequence of the *S. bacillaris* type strain CBS 9494 reported here, as well as additional specific molecular tools for population analyses (5, 15, 21–23), will contribute to the knowledge of the genetic and geographic diversity of this species as well as its life cycle (21).

In this study, total DNA from *S. bacillaris* type strain CBS 9494 was prepared using a genomic DNA buffer set and Genomic-tip 100/G kits (Qiagen). Genomic DNA (1 µg) was enzymatically fragmented (10 min) and size selected (315 bp) using the Pippin Prep instrument (Sage Science); a genomic library was generated with the Ion Xpress Plus fragment library kit (ThermoFisher Scientific). The genome sequence (122-fold genome coverage), obtained on the Ion Torrent PGM platform (Life Technologies), was trimmed based on a Phred-type quality threshold score of Q20 (QPhred = 20) and a length threshold of 50 bp using CLC Genomics Workbench version 7.0.3 (CLC bio). A total of

**Received** 22 June 2018 **Accepted** 26 June 2018 **Published** 26 July 2018

**Citation** Rosa AL, Miot-Sertier C, Laizet Y, Salin F, Sipiczki M, Bely M, Masneuf-Pomarede I, Albertin W. 2018. Draft genome sequence of the *Starmerella bacillaris* (syn., *Candida zemplinina*) type strain CBS 9494. *Microbiol Resour Announc* 7:e00872-18. https://doi.org/10.1128/MRA.00872-18.

**Editor** John J. Dennehy, Queens College

**Copyright** © 2018 Rosa et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

Address correspondence to Alberto Luis Rosa, alrosa@ucc.edu.ar.

<footnote>ªLaboratorio de Genética y Biología Molecular, IRNASUS-CONICET, Facultad de Ciencias Químicas, Universidad Católica de Córdoba, Córdoba, Argentina</footnote>

<footnote>ᵇISV, Oenology Research Unit EA 4577, USC 1366 INRA, Bordeaux INP, University of Bordeaux, Villenave d'Ornon, France</footnote>

<footnote>ᶜUMR Biodiversity of Genes and Ecosystems, Genomics Platform, INRA, Cestas, France</footnote>

<footnote>ᵈDepartment of Genetics and Applied Microbiology, University of Debrecen, Debrecen, Hungary</footnote>

<footnote>ᵉBordeaux Sciences Agro, Gradignan, France</footnote>

<footnote>ᵉENSCBP, Bordeaux INP, Pessac, France</footnote>
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5,698,579 reads (mean size of 200 bp) were used for *de novo* assembly with Newbler version 2.7 (454 Life Sciences), which resulted in 200 contigs longer than 200 bp (mean, 108,648 bp; maximum, 649,352 bp; N<sub>50</sub>, 175,910 bp). The genome of *S. bacillaris* type strain 9494 was 9.3 Mb with a G+C content of 39.4%, similar to the recently available genome data for *S. bacillaris* strains FRI751 (24) and PAS13 (25). The *S. bacillaris* type strain CBS 9494 genome sequence reported here will constitute a reference sequence for studying evolutionary and phylogenetic aspects, as well as the geographic biodiversity, of this genetically heterogeneous species (21).

**Data availability.** This whole-genome shotgun project has been deposited at DDBJ/ENA/GenBank under the accession number **QLKO00000000**. The version described in this paper is the first version, **QLKO01000000**.

## ACKNOWLEDGMENTS

This research was funded in part by the Biolaffort and Pernod SA non-Saccharomyces project (France) (to M.B., I.M.-P., and W.A.) and by grant PICT-2014-3113 to A.L.R. from FONCYT (Argentina). The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

A.L.R. is a principal investigator from CONICET (Argentina).

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<figure><img src="image_4.png" /></figure>

# Correction for Rosa et al., “Draft Genome Sequence of the *Candida zemplinina* (syn., *Starmerella bacillaris*) Type Strain CBS 9494”

Alberto Luis Rosa,ª Cécile Miot-Sertier,ᵇ Yec'han Laizet,ᶜ Franck Salin,ᶜ Matthias Sipiczki,ᵈ Marina Bely,ᵇ Isabelle Masneuf-Pomarede,ᵇ,e Warren Albertinᵇ,f

Volume 7, no. 3, e00872-18, 2018, https://doi.org/10.1128/MRA.00872-18. Page 1:
The article title should read as given above.

Page 1, line 1: "*Starmerella bacillaris* (syn., *Candida zemplinina*)" should read "*Candida zemplinina* (syn., *Starmerella bacillaris*)."

Pages 1 and 2: "*S. bacillaris*" should read "*C. zemplinina*" throughout.

**Published** 13 September 2018

**Citation** Rosa AL, Miot-Sertier C, Laizet Y, Salin F, Sipiczki M, Bely M, Masneuf-Pomarede I, Albertin W. 2018. Correction for Rosa et al., "Draft genome sequence of the *Candida zemplinina* (syn., *Starmerella bacillaris*) type strain CBS 9494." *Microbiol Resour Announc* 7:e01116-18. https://doi.org/10.1128/MRA.01116-18.

**Copyright** © 2018 Rosa et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

Address correspondence to Alberto Luis Rosa, alrosa@ucc.edu.ar.

<footnote>ªLaboratorio de Genética y Biología Molecular, IRNASUS-CONICET, Facultad de Ciencias Químicas, Universidad Católica de Córdoba, Córdoba, Argentina</footnote>

<footnote>ᵇISV, Oenology Research Unit EA 4577, USC 1366 INRA, Bordeaux INP, University of Bordeaux, Bordeaux, Villenave d'Ornon, France</footnote>

<footnote>ᶜUMR Biodiversity of Genes and Ecosystems, Genomics Platform, INRA, Cestas, France</footnote>

<footnote>ᵈDepartment of Genetics and Applied Microbiology, University of Debrecen, Debrecen, Hungary</footnote>

<footnote>ᵉBordeaux Sciences Agro, Gradignan, France</footnote>

<footnote>ᵉNSCBP, Bordeaux INP, Pessac, France</footnote>