# New species in the papaya ringspot virus cluster: insights into the evolution of the PRSV lineage

Cecile Desbiez, Gustavo Romay, Pauline Millot, Catherine Wipf-Scheibel, Gasim Dafalla, Hervé Lecoq

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Cecile Desbiez, Gustavo Romay, Pauline Millot, Catherine Wipf-Scheibel, Gasim Dafalla, et al. New species in the papaya ringspot virus cluster: insights into the evolution of the PRSV lineage. 16. Rencontres de Virologie Végétale (RVV 2017), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD). FRA., Jan 2017, Aussois, France. 137 p. hal-01605294

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Submitted on 2 Jun 2020

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# New species in the *papaya ringspot virus* cluster: insights into the evolution of the PRSV lineage

C. Desbiez<fnref n="1" />, G. Romay<fnref n="2" />, P. Millot<fnref n="1" />, C. Wipf-Scheibel<fnref n="1" />, G. Dafalla<fnref n="3" />, H. lecoq<fnref n="1" />

More than 70 viruses infect cucurbits worldwide. Among the most common and agronomically important are several potyviruses: *zucchini yellow mosaic virus* (ZYMV), *watermelon mosaic virus* (WMV) and *papaya ringspot virus* (PRSV). Besides these viruses that have a worldwide distribution and present distinct biological, serological and molecular features, several viruses closely related to PRSV that could constitute highly divergent strains of this virus or related species have been defined, and could represent new threats for agriculture.

Biological, serological and molecular studies, as well as analysis of database sequences, were performed in order to characterize the different species in the PRSV cluster and study their geographic distribution and population structure (1,2). Isolates from worldwide origins were tested serologically (DAS-ELISA) or molecularly (RT-PCR with specific or generic primers) for the presence of the different viruses.

Based on their molecular properties, at least 10 distinct species exist in the “PRSV cluster” (3). Their geographic range, field prevalence and agronomic impacts are highly contrasted: PRSV and to a lesser extent *zucchini tigre mosaic virus* (ZTMV) have a worldwide distribution, *Moroccan watermelon mosaic virus* (MWMV) is widespread in Africa and emerging in the Mediterranean Basin but not reported elsewhere, while the other viruses appear endemic to one country even though they can be maintained locally over several decades. The geographic structure and molecular diversity patterns of the putative and acknowledged species suggest that the PRSV-like cluster originated in the Old World, with an important diversification in Africa.

The number of different potyviruses in the “PRSV cluster” is probably still underestimated. Excepting PRSV and to a lower extent ZTMV and MWMV, species in the “PRSV-cluster” seem to have a narrow geographic range and low epidemiological impact so far. At least two species appear endemic to Sudan, with contrasted frequencies. These results show that currently unimportant viruses may emerge if the local conditions change or if they are introduced in a more favourable environment.

## Références :

[1] Quiot-Douine, L., Lecoq, H., Quiot, J.-B., Pitrat, M. and Labonne, G. (1990) Serological and biological variability of virus isolates related to strains of *papaya ringspot virus*. Phytopathology, 80, 256-263.

[2] Lecoq, H., Dafalla, G., Desbiez, C., Wipf-Scheibel, C., Delecolle, B., Lanina, T., Ullah, Z. and Grumet, R. (2001) Biological and molecular characterization of *Moroccan watermelon mosaic virus* and a potyvirus isolate from Eastern Sudan. Plant Disease, 85, 547-552.

[3] Romay, G., Lecoq, H. and Desbiez, C. (2014) *Zucchini tigre mosaic virus* is a distinct species into the *papaya ringspot virus* cluster: Molecular and biological insights. Archives of Virology, 159, 277-289.

<footnote n="1">¹Inra, UR0407 Pathologie Végétale, 84140 Montfavet, France</footnote>

<footnote n="2">² Université catholique de Louvain, Earth and Life Institute, Phytopathology, Croix du Sud 2 Bte L07.05.03, 1348 Louvain-la-Neuve, Belgium</footnote>

<footnote n="3">³Plant Pathology Center, University of Gezira, Wad Medani, Sudan</footnote>
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16ièmes Rencontres de Virologie Végétale

15 - 19 JANVIER 2017 • AUSSOIS - FRANCE

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Aussois
du 15 au 19 janvier 2017