# Preserved organic matter in a fossil Ocean Continent Transition in the Alps: the example of Totalp, SE Switzerland

Tsvetomila Mateeva, George Wolff, Gianreto Manatschal, Suzanne Picazo, Nick Kusznir, John Wheeler

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Tsvetomila Mateeva, George Wolff, Gianreto Manatschal, Suzanne Picazo, Nick Kusznir, et al. Preserved organic matter in a fossil Ocean Continent Transition in the Alps: the example of Totalp, SE Switzerland. Swiss Journal of Geosciences, 2017, 110 (2), pp.457 - 478. 10.1007/s00015-017-0266-3. hal-01864639

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# Preserved organic matter in a fossil Ocean Continent Transition in the Alps: the example of Totalp, SE Switzerland

Tsvetomila Mateeva¹, George A. Wolff¹, Gianreto Manatschal², Suzanne Picazo³, Nick J. Kusznir¹, John Wheeler¹

Received: 31 May 2016/Accepted: 13 February 2017/Published online: 2 March 2017
© The Author(s) 2017. This article is published with open access at Springerlink.com

**Abstract** Evidence from ultraslow spreading mid-ocean ridges and both fossil and present-day Ocean-Continent Transitions (OCT) demonstrates that mantle serpentinization resulting from the interaction of mantle rock and water during tectonic exhumation is widespread. Observations at white smokers in modern ocean settings suggest that methane produced by serpentinization can support methanotrophic bio-systems, which use methane as the only source of carbon. An important question is whether such bio-systems are more generally pervasive in their association with serpentinized mantle in the subsurface. In this study, we examined whether there is evidence for such a methanotrophic system in exhumed serpentinized mantle at a magma-poor rifted continental margin, by probing for characteristic biological markers in these and associated sedimentary rocks in the Totalp unit of SE Switzerland. This unit represents a remnant of the former OCT of the southern Alpine Tethyan margin and was chosen because of its mild Alpine tectonic and low-grade metamorphic overprint during Alpine orogeny, hence giving potential for the preservation of indigenous organic matter (OM). Totalp samples are characterized by low organic carbon contents of 11–647 ppm. The majority of the samples contain hydrocarbons in the form of n-alkanes in the range C₁₇–C₃₆. Some

sediments contain isoprenoids, for example pristane and phytane and a suite of steranes that are consistent with a marine origin for the OM preserved in the rocks. Traces of marine planktonic and bacterial OM are preserved in the serpentinized mantle and overlying sediments of this ancient Tethyan OCT, but there is no evidence that the OM has been generated from methanotrophic bio-systems.

**Keywords** Serpentinization · Organic matter · Totalp unit · Biogeochemistry · Ocean Continent Transition (OCT)

## 1 Introduction

Serpentinization is an important metamorphic exothermic hydration process potentially contributing chemical energy for anaerobic life, as well as thermal energy at oceanic hydrothermal vents (e.g. Shock et al. 2002; Jamtveit and Hammer 2012). Serpentinization converts olivine and pyroxene to serpentine, other Fe–Mg minerals (magnetite, brucite, talc) and free molecular hydrogen (Eq. 1). The Mg component of olivine may hydrate as follows:

$$2\text{Mg}_2\text{SiO}_4 + 3\text{H}_2\text{O} \rightarrow \text{Mg}_3\text{Si}_2\text{O}_5(\text{OH})_4 + \text{Mg}(\text{OH})_2 \quad (1)$$

The Fe component of olivine contributes to forming serpentine by an analogous reaction and/or by reducing water

$$6\text{Fe}_2\text{SiO}_4 + 7\text{H}_2\text{O} \rightarrow 3\text{Fe}_3\text{Si}_2\text{O}_5(\text{OH})_4 + \text{Fe}_3\text{O}_4 + \text{H}_2 \quad (2)$$

It has been proposed (e.g. McCollom 2013) that molecular hydrogen can then reduce CO₂ derived from carbonate and hydrogen carbonate in sea-derived pore waters to methane (CH₄) at high pressure and temperature, via a Fischer–Tropsch-like reaction (Eq. 3).

Editorial handling: C. Sue and S. Schmid.

Tsvetomila Mateeva
mateeva@liv.ac.uk

¹ Department of Earth, Ocean and Ecological Sciences, University of Liverpool, Liverpool L69 3BX, UK

² CNRS-IPGS-EOST, Université de Strasbourg, 1 rue Blessig, 67084 Strasbourg, France

³ University of Lausanne, 1015 Lausanne, Switzerland

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$$CO_2 + 4H_2 \rightarrow CH_4 + 2H_2O \quad (3)$$

Serpentinized mantle rocks exposed along slow to ultra-slow-spreading Mid-Ocean Ridges (MOR) show positive anomalies of methane and hydrogen in the overlying water column above active tectonic zones (Rona et al. 1987; Charlou et al. 1988; Rona et al. 1992; Bougault et al. 1993; Charlou and Donval 1993; Charlou et al. 1998; Gràcia et al. 2000; Kelley and Shank 2010). The abiotically produced methane can be anaerobically oxidised by methanotrophic bacteria using sulphate as the electron acceptor (Eq. 4).

$$CH_4 + SO_4^{2-} \rightarrow HCO_3^- + HS^- + H_2O \quad (4)$$

Sulphate reducers are one of the dominant bacterial populations at hydrothermal vents (McCollom and Shock 1997). Furthermore, methanotrophic bacteria have been identified at Lost City (mid-Atlantic Ocean), a low-temperature alkaline hydrothermal vent supported by energy derived from the formation of serpentinite (Hinrichs et al. 2000; Kelley et al. 2001; Orphan et al. 2001; Kelley et al. 2005). Recently, bacterial anaerobic nitrate oxidation of methane has been demonstrated in the laboratory (Haroon et al. 2013; Arshad et al. 2015), using two different microorganisms (Raghobarsing et al. 2006); this may also occur at the MOR methane sources, but is likely to be a minor pathway as nitrate concentrations in seawater are significantly lower than sulphate (µM vs. mM, respectively).

Recent studies have focussed on the relationship between serpentinization and organic compounds, mainly methane in the laboratory (e.g. McCollom and Seewald 2013; Etiope and Ionescu 2014), at present-day serpentinite-hosted hydrothermal vents (e.g. Kelley et al. 2005; Delacour et al. 2008; Proskurowski et al. 2008), mud volcanoes (e.g. Mottl et al. 2003; Holm et al. 2006), and exhumed serpentinite mantle domains with high H₂ concentrations and high pH (e.g. Cardace et al. 2013). High concentrations of OM found at the Mid-Atlantic Ridge (MAR; 4–6°N) were associated with serpentinized peridotite rather than with the hydrothermal vents (Ménez et al. 2012).

Hence, the present study uses an organic geochemical approach to quantify OM in the exhumed mantle from an OCT in order to better understand the relationship between OM and mantle serpentinization. We selected the Totalp unit exposed in the Eastern Swiss Alps, which represents a remnant of the fossil Tethyan OCT emplaced during the Alpine orogeny (Fig. 1a; Weissert and Bernoulli 1985; Manatschal et al. 2003; Picazo et al. 2013). We searched for biomarkers or molecular remains of former living organisms, specifically hydrocarbons with an origin consistent with anaerobic methane oxidation (e.g. crocetane; Blumenberg et al. 2004). A wider suite of biomarkers was

used to determine source and thermal maturity of OM preserved in the rocks.

## 2 Regional geological setting of the Totalp unit

The Totalp unit is located north of Davos in SE Switzerland. It is part of the Tethyan OCT (Fig. 1b). The peculiarity of the Totalp unit is that it experienced little Alpine deformation and only a low grade Alpine metamorphic overprint, not exceeding 100–150 °C, i.e. prehnite-pumpellyite grade (Peters 1968; Früh-Green et al. 1990). The Totalp unit consists of two Alpine tectonic units namely the Upper and Lower Ultramafic Totalp sub-units (e.g. Picazo et al. 2013). These are mainly composed of serpentinized peridotite exhumed at the seafloor during Jurassic times and ophicalcites that occur at the top basement; they can be found re-worked into the overlying Jurassic marine sediments (Fig. 1c). In addition, the primary contacts between the exhumed serpentinized mantle, the ophicalcites and the oceanic sediments are well-preserved (Weissert and Bernoulli 1985).

### 2.1 Pre-Alpine and Alpine geological and thermal history

The serpentinized peridotites of the Totalp unit were exhumed to the Jurassic seafloor during final late Middle Jurassic rifting (Peters and Stettler 1987; Bernoulli et al. 2003). Later, during the Alpine orogeny, the exhumed mantle rocks were first tectonically emplaced within a Late Cretaceous E-W directed nappe stack before being thrusted during the Tertiary collision over the European units forming the present-day Alpine orogen (Weissert and Bernoulli 1985; Früh-Green et al. 1990; Manatschal et al. 2003). The serpentinized peridotites in Totalp are interpreted as derived from fertile subcontinental lithospheric mantle (Manatschal et al. 2001; Müntener et al. 2010; van Acken et al. 2010), similar to other described remnants of fossil OCTs from the Alps (e.g. Platta; Desmurs et al. 2002; Malenco in the Italian Alps; Müntener et al. 2004). Extensional faults and unroofing of their footwalls are responsible for the mantle exposure at the seafloor (Picazo et al. 2013).

### 2.2 Lithologies

The Totalp unit consists mainly of serpentinized peridotites, ophicalcites and post-rift sediments (Figs. 1 and 2; Table 1). This association is typical of an OCT across magma-poor margins where magmatic rocks are often either very rare or absent. Three types of serpentinized mantle rocks were identified:
<figure><img src="image_5.png" /><figcaption>**Fig. 1** Maps showing location and geology of the Totalp area. **a** Location of the Totalp area in the tectonic map of the Alps (from Schmid et al. 2004; modified by Mohn et al. 2010). **b** Location of Totalp as a part of the South Penninic units in Grisons (Manatschal</figcaption></figure>

et al. 2003). **c** Geological map of Totalp (modified from Picazo et al. 2013) showing location of the analysed samples as well as the distribution of the major lithologies and samples described in this study (for description of samples see Table 1)

(i) Massive serpentinized peridotites, which preserve mantle textures and mainly consist of serpentinized spinel-lherzolite. Locally these rocks also contain pyroxenite and amphibole- and chlorite-rich layers (Picazo et al. 2013).

(ii) During exhumation, the serpentinized peridotites are affected by localization of the deformation and intensive fluid circulation leading to complete serpentinization of fault-rocks, including serpentinite gouges, serpentinite cataclasites and foliated cataclasites (Picazo et al. 2013). These rocks are best exposed in the Obersasställi area, and they occur in the uppermost 150 m of the exhumed mantle in the footwall of a Jurassic extensional detachment fault (Figs. 1 and 2; Picazo et al. 2013).

(iii) Veins of serpentine that have been interpreted to result from later serpentinization during low-grade Alpine metamorphism, as suggested by the oxygen isotopes (Früh-Green et al. 1990). Some serpentine veins may result from the percolation

of meteoric water associated with regional metamorphism (Burkhard and O'Neil 1988).

Ophicalcites are complex rocks that are made of serpentinite and calcite and represent the result of different processes (Bernoulli and Weissert 1985; Lemoine et al. 1987). They either result from the total to partial in situ replacement of serpentine by carbonate, tectonic processes related to exhumation and hydrothermal systems, and/or cementation and filling of fractures by sediments (e.g. neptunian dykes of Bernoulli and Jenkyns 2009). These processes occur at or near the seafloor and are often associated with hydrothermal fluid circulation at temperatures of 100–150 °C (Früh-Green et al. 1990; Picazo et al. 2013). Most ophicalcites are formed under static (non tectonic) conditions within 20 m of the paleo-seafloor (Picazo et al. 2013).

Sedimentary ophicalcites at Totalp include neptunian dykes and debris-flows (Fig. 2). The neptunian dykes are carbonate veins filled with pink or grey carbonate (pelagic sediments or mechanically reworked cements), filling fractures in the exhumed mantle. These dykes are typically located in the tectonized, serpentinized peridotites forming
Log of Totalp exhumed mantle and sedimentary cover

<figure><img src="image_6.png" /><figcaption>**Fig. 2** Sketch showing the lithologies and the stratigraphy of the Totalp area (Weissert and Bernoulli 1985; Früh-Green et al. 1990; Manatschal et al. 2003; Picazo et al. 2013)</figcaption></figure>

the uppermost few meters of the exhumed mantle (Bernoulli and Weissert 1985; Picazo et al. 2013). The second main type, the tectono-sedimentary ophicalcites, include cemented clasts of serpentinite, gabbro and continental basement (Manatschal and Bernoulli 1999; Bernoulli et al. 2003; Picazo et al. 2013).

The sediments overlying the ophicalcite are mainly pelagic deposits of Late Jurassic to Early Cretaceous age, some of which have been reworked (most likely by ocean

bottom currents) (Weissert and Bernoulli 1985). Red shales are overlain by radiolarian cherts and grey micritic limestones intercalated with claystones (e.g. Radiolarite Formation and Calpionella or Aptychus limestone Formation of Weissert and Bernoulli 1985). The top of the sequence is formed by black siliceous shales that are characteristic of poorly oxygenated bottom waters during the Early Cretaceous (Weissert et al. 1985; Weissert and Bernoulli 1985).
<table><caption>**Table 1** Results by lithology including mineralogy and texture observed in hand specimens and the texture on the calcites used for the isotopic analyses (δ¹³C<sub>VPDB</sub>, δ¹⁸O<sub>VPDB</sub>)</caption><thead><tr><th>Rock type</th><th>Sample number</th><th>Coordinates (UTM) X (m)</th><th>Y (m)</th><th>Locality</th><th>Macroscopic description</th><th>Mineralogical description</th><th>Texture</th><th>Carbonate texture</th><th>δ¹³C<sub>VPDB</sub> (‰) Ca veins</th><th>δ¹⁸O<sub>VPDB</sub> (‰) Ca veins</th></tr></thead><tbody><tr><td><strong>Serpentinite</strong></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>Massive serpentine</td><td>5</td><td>561895</td><td>5188662</td><td>Parsennfurgga</td><td>Massive black serpentine with white angular borders defining the exoperidotite minerals, small green chrysotile veinlets and white carbonate veinlets</td><td>Olivine completely replace by serpentine minerals, phantoms of pyroxene, magnetite, later carbonate vein: calcite veins with only one stage of crystal growing</td><td>Mesh<sup>a</sup></td><td>Calcite vein, 1st generation</td><td>0.11</td><td>-10.2</td></tr><tr><td></td><td>16</td><td>561522</td><td>5188399</td><td>2442 m. Obersasställi-Parsennfurgga</td><td></td><td>Mainly mesh texture with rare ribbon texture; randomly dispersed anhedral magnetite grain; later veins of chrysotile</td><td>Clast supported breccia<sup>a</sup></td><td>Calcite vein, 1st generation</td><td>0.03</td><td>-11.75</td></tr><tr><td><strong>Serpentinite with numerous calcite veins</strong></td><td>24</td><td>561514</td><td>5188009</td><td>Obersasställi</td><td>Broken serpentine clasts from a first generation of greyish deformed calcite veins crosscut almost perpendicularly by a second generation of white calcite veins</td><td>The serpentinite is lizardite containing also talc and calcite; the red colour is due to hematite (4%)</td><td>Red serpentinite breccia</td><td>Calcite vein, 2st generation</td><td>1.37</td><td>-11.3</td></tr><tr><td></td><td>26</td><td>561504</td><td>5188010</td><td>2442 m. Obersasställi-Parsennfurgga</td><td></td><td>No thin section or XRD</td><td>Fine grained</td><td></td><td></td><td></td></tr><tr><td></td><td>44</td><td>561518</td><td>5188415</td><td></td><td></td><td>No thin section or XRD</td><td>Fine grained</td><td></td><td></td><td></td></tr><tr><td></td><td>47</td><td>561540</td><td>5188427</td><td></td><td></td><td>No thin section or XRD</td><td>Fine grained</td><td></td><td></td><td></td></tr><tr><td><strong>Red serpentine</strong></td><td>71</td><td>561588</td><td>5188095</td><td>2442 m. Obersasställi-Parsennfurgga</td><td>Red matrix with dark-black deformed clasts/veins with numerous calcite veins, also contain dark green serpentine</td><td>Black fine-grained deformed serpentine with equant serpentine grain following and distributed systematically and fine short veinlets (chrysotile; calcite)</td><td>Red serpentinite with subangular serpentine clasts showing no prefered orientation</td><td>Calcite vein, 2st generation</td><td>1.37</td><td>-11.3</td></tr><tr><td></td><td>69</td><td>561929</td><td>5187152</td><td>Obersasställi</td><td></td><td>No thin section or XRD</td><td>Fine grained</td><td></td><td></td><td></td></tr><tr><td><strong>Serpentinite gouge</strong></td><td>70</td><td>561929</td><td>5187152</td><td>Obersasställi</td><td>Black fine-grained deformed serpentine with equant serpentine grain following and distributed systematically and fine short veinlets (chrysotile; calcite)</td><td>Black serpentinite with subangular serpentine clasts showing no prefered orientation</td><td>Red serpentinite with sub- rounded anhedral white- grayish peridotite mineral (pyroxene) and small chrysotile veins</td><td>Calcite vein, 2st generation</td><td>1.37</td><td>-11.3</td></tr><tr><td><strong>Serpentinite cataclasite</strong></td><td>77</td><td>561482</td><td>5187004</td><td>Weissflußhoch</td><td></td><td>No thin section or XRD</td><td>Fine grained</td><td></td><td></td><td></td></tr><tr><td><strong>Massive serpentine*</strong></td><td>78</td><td>561482</td><td>5187004</td><td></td><td></td><td>No thin section or XRD</td><td>Fine grained</td><td></td><td></td><td></td></tr></tbody></table>
<table><caption>Table 1 continued</caption><thead><tr><th>Rock type</th><th>Sample number</th><th>Coordinates (UTM) X (m)</th><th>Y (m)</th><th>Locality</th><th>Macroscopic description</th><th>Mineralogical description</th><th>Texture</th><th>Carbonate texture</th><th>δ<sup>13</sup>C<sub>VPDB</sub> (%) Ca veins</th><th>δ<sup>18</sup>O<sub>VPDB</sub> (%) Ca veins</th></tr></thead><tbody><tr><td colspan="11"><strong>Sedimentary ophicalcite</strong></td></tr><tr><td>Limestone (Neptunian dykes)</td><td>1</td><td>561543</td><td>5188408</td><td>2442 m. Obersasställi Parsemnfurga</td><td>Fine micritic pink and red pelagic limestone; serpentine minerals as clasts and veins</td><td>Three types of carbonate crystal growing; the matrix composed of rounded to angular coarse grains with hornblend floating randomly in the matrix</td><td>Porphyroclastic<sup>a</sup></td><td>Calcite vein, 2nd generation</td><td>1.56</td><td>-6.4</td></tr><tr><td>Tectono-sedimentary ophicalcite</td><td>21</td><td>561520</td><td>5188015</td><td>Obersasställi</td><td>Red fine carbonate matrix with black anhedral serpentine broken clasts crosscutted by calcite veins</td><td>Serpentinites clasts broken by several generation of carbonate veins; several stage of crystal growing for the carobnates;</td><td>Fine grained with clasts<sup>a</sup></td><td>Calcite vein, 1st generation</td><td>0.32</td><td>-11.7</td></tr><tr><td></td><td>25</td><td>561511</td><td>5188009</td><td>Parsemnfurga</td><td></td><td></td><td></td><td>Calcite vein, 1st generation</td><td>0.58</td><td>-10.9</td></tr><tr><td></td><td>39</td><td>561515</td><td>5188439</td><td>2442 m. Obersasställi</td><td></td><td></td><td></td><td>Calcite vein, 2nd generation</td><td>1.78</td><td>-7.1</td></tr><tr><td></td><td>41</td><td>561572</td><td>5188406</td><td>Parsemnfurga</td><td></td><td></td><td></td><td>Calcite vein, 1nd and 2nd gener</td><td>0.42; 1.07</td><td>-10.3; -8.6</td></tr><tr><td></td><td>42</td><td>561572</td><td>5188406</td><td></td><td></td><td></td><td></td><td>Calcite vein, undetermined generation</td><td>1.86</td><td>-6.2</td></tr><tr><td></td><td>46</td><td>561540</td><td>5188418</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td></td><td>66</td><td>564260</td><td>5190402</td><td>Near Grüenhorn</td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>Reworked tec-sed ophicalcite</td><td>9</td><td>562586</td><td>5188578</td><td>Parsemnfurga-Parsennhütte</td><td>Pink carbonate with randomly orientated elongated clast of ophicalcite (serpentinite + carbonate); numerous calcite veins</td><td>No thin section or XRD</td><td>reworked<sup>a</sup></td><td>Vein supported breccia</td><td>1.60</td><td>-10.6</td></tr><tr><td>Vein supported serpentine breccia</td><td>72</td><td>561929</td><td>5187152</td><td>2442 m. Obersasställi</td><td>Centimetric angular clasts of green serpentine tectonically broken by calcite veins</td><td>No thin section or XRD</td><td></td><td>Calcite vein, undetermined generation</td><td></td><td></td></tr></tbody></table>
<table><caption>Table 1 continued</caption><thead><tr><th>Rock type</th><th>Sample number</th><th>Coordinates (UTM) X (m)</th><th>Y (m)</th><th>Locality</th><th>Macroscopic description</th><th>Mineralogical description</th><th>Texture</th><th>Carbonate texture</th><th>δ<sup>13</sup>C<sub>Vp</sub><sub>dB</sub> (%) Ca veins</th><th>δ<sup>18</sup>O<sub>Vp</sub><sub>dB</sub> (%) Ca veins</th></tr></thead><tbody><tr><td rowspan="2">Sediments</td><td>Radiolarite</td><td>62</td><td>564212</td><td>5190386</td><td>Near Grüenhorn from sulphide rich outcrop</td><td>Hard fine grained red siliceous rock; post deposition quartz vein perpendicular to the geometry of deposition</td><td>No thin section or XRD</td><td>Fine grained</td><td>Post-deposit calcite vein</td><td>-0.79</td><td>-6.4</td></tr><tr><td>Siliceous shale</td><td>68</td><td>564252</td><td>5190393</td><td>Near Grüenhorn from sulphide rich outcrop</td><td>Greyish-reddish fine grained siliceous rock, great porosity and numerous fractures</td><td>Composed mainly by quartz (46%), plagioclase(30%), chlorite, muscovite and hematite</td><td>Fine grained</td><td></td><td></td><td></td></tr><tr><td rowspan="2">Limestone</td><td></td><td>60</td><td>564212</td><td>5190386</td><td></td><td></td><td>Composed mainly by quartz (46%), plagioclase(30%), chlorite, muscovite and hematite</td><td>Fine grained</td><td></td><td></td><td></td></tr><tr><td></td><td>61</td><td>564212</td><td>5190386</td><td></td><td></td><td>Composed mainly by quartz (46%), plagioclase(30%), chlorite, muscovite and hematite</td><td>Fine grained</td><td></td><td></td><td></td></tr><tr><td rowspan="2">Limestone</td><td></td><td>15</td><td>564049</td><td>5189517</td><td>Gotschnagrat</td><td>Red and grey fine grained intercalated siliceous shale with deformed quartz veins</td><td>Fine grained</td><td></td><td></td><td></td><td></td></tr><tr><td></td><td>18</td><td>564213</td><td>5189544</td><td></td><td></td><td>Composed mainly by quartz (46%), plagioclase(30%), chlorite, muscovite and hematite</td><td>Fine grained</td><td></td><td></td><td></td></tr><tr><td rowspan="2">Limestone</td><td></td><td>10</td><td>562494</td><td>188558</td><td>Parsennfurgä- Parsemmhütte</td><td>Grey limestone with numerous calcite veins of two generation almost perpendicular to each other</td><td>Fine grained</td><td></td><td></td><td></td><td></td></tr><tr><td></td><td>19</td><td>563790</td><td>5189694</td><td>Gotschnagrat</td><td>Grey limestone with fine greyish veinlets and rare short calcite veins with pink pelagic limestone (hematite) in places</td><td>Fine grained</td><td></td><td></td><td></td><td></td></tr><tr><td rowspan="2">Carbonate (sulphide rich outcrop)</td><td></td><td>53</td><td>563144</td><td>5188716</td><td>South from Parsemmhütte</td><td>Grey limestone with fine greyish veinlets and rare short calcite veins with pink pelagic limestone (hematite) in places</td><td>Fine grained</td><td></td><td></td><td></td><td></td></tr><tr><td></td><td>54</td><td>563140</td><td>5188707</td><td></td><td></td><td>Composed mainly by quartz (46%), plagioclase(30%), chlorite, muscovite and hematite</td><td>Fine grained</td><td></td><td></td><td></td></tr><tr><td rowspan="2">Carbonate (sulphide rich outcrop)</td><td></td><td>56</td><td>563140</td><td>5188707</td><td></td><td></td><td>Composed mainly by quartz (46%), plagioclase(30%), chlorite, muscovite and hematite</td><td>Fine grained</td><td></td><td></td><td></td></tr><tr><td></td><td>57</td><td>563144</td><td>5188716</td><td></td><td></td><td>Composed mainly by quartz (46%), plagioclase(30%), chlorite, muscovite and hematite</td><td>Fine grained</td><td></td><td></td><td></td></tr><tr><td rowspan="2">Carbonate (sulphide rich outcrop)</td><td></td><td>58</td><td>563144</td><td>5188716</td><td></td><td></td><td>Composed mainly by quartz (46%), plagioclase(30%), chlorite, muscovite and hematite</td><td>Fine grained</td><td></td><td></td><td></td></tr><tr><td></td><td>63</td><td>564212</td><td>5190386</td><td>Near Grüenhorn</td><td>Carbonate rock with deformed quartz veins; microscopic grain of pyrite everywhere except in the quartz and calcite veins</td><td>Fine grained</td><td>Fine grained</td><td></td><td></td><td></td></tr><tr><td rowspan="2">Carbonate (sulphide rich outcrop)</td><td></td><td>64</td><td>564212</td><td>5190386</td><td></td><td></td><td>Carbonate rock with deformed quartz veins; microscopic grain of pyrite everywhere except in the quartz and calcite veins</td><td>Fine grained</td><td></td><td></td><td></td></tr><tr><td></td><td>65</td><td>564252</td><td>5190393</td><td></td><td></td><td>Very fractured sample with greater porosity</td><td>Fine grained</td><td></td><td></td><td></td></tr></tbody></table>

The vein generation is defined from the specific example and may not correlate across all samples

* All lithology are from the lower sub-unit with the exception of the massive serpentinite (samples 77, 78)

a Thin section of the lithology is observed
<figure><img src="image_7.png" /><figcaption>**Fig. 3** The sulphide bearing outcrop in the Totalp area showing weathered lithology. Red circles are the sampling location in this outcrop. In the line-drawing on the right, light red identifies radiolarian cherts, dark red siliceous shale and yellow sulphate rich carbonate, rich in silica. a Zoom of outcrop. b Sample 65 is composed</figcaption></figure>

by calcite 50%. 25% quartz and is highly weathered. c Sample shows visible hematite crystals and calcite as the major mineral. d Sample 60 shows a siliceous rock with 3 different colours composed by 83% quartz, 10% albite and pyrite, illite and chlorite

North east of Totalp, exposed on a topographic cliff near Gotschnagrat (Figs. 1 and 2), is a weathered outcrop with visible pyrite on the top of the radiolarian cherts and siliceous claystones (Fig. 3; Weissert and Bernoulli 1985). This outcrop has been described by Früh-Green et al. (1990) as a zone of pyrite mineralization associated with radiolarian cherts. The radiolarites contain quartz, illite, hematite and chlorite (Weissert and Bernoulli 1985). The sulphidized outcrop has a bulk chemistry different from its surrounding and could have been formed during early diagenesis or metasomatism of amorphous silica or limestone (Berner 1984; Williams et al. 1985). Alternatively,

the sulphide could be related to a fossil hydrothermal system (e.g. Styrt et al. 1981, Beard and Hopkinson 2000; Zeng et al. 2015). Early diagenetic reactions producing pyrite depend inter alia on a source of OM as electron donor (Fig. 3b); the high present-day porosity of the rock (Fig. 3c) could be produced by weathering of calcite (Berner 1984). Hydrothermal vent systems are characterized by pelagic sediment accumulation alongside ferrous oxides (i.e. radiolarian chert) of hydrothermal origin which can potentially be preserved (Haymon 1989; Montgomery and Kerr 2009). The second most common mineral in the sulphide bearing samples is quartz that may have
originated from amorphous silica or opal, which are com-
mon in hydrothermal vents (e.g. MAR- mount Saldanha
36°30′N; Dias and Barriga 2006, 25°48′N Rona 1984,
24°21′N; Rona 1984; Kane 23°35′N Fracture zone; Kelley
and Delaney 1987) reflecting a large input of hydrothermal
silica (Dias and Barriga 2006). There is no evidence of
fossilized worm tubes associated with the zone of pyrite
mineralization; these are characteristic of inactive white
smokers (Haymon 1983) and so the origin of this formation
remains unclear.

**3 Sample collection, preparation and analysis**

**3.1 Sample collection**

A total of 47 samples were selected from the Totalp unit for geological and organic geochemical studies (Table 1). We collected samples from the three main lithologies: serpentinized mantle rock (lizardite, serpentinite gouge and cataclasite), ophicalcites (neptunian dykes and tectono-sedimentary breccias) and associated sediments (Figs. 1 and 2; Table 1). Samples were oriented, geo-referenced and collected using a geological hammer or a hand drill using water as lubricant. On collection the samples were wrapped in pre-combusted foil (400 °C) for return to the laboratory.

**3.2 Sample preparation**

In order to avoid contamination with modern material the
samples were cleaned with de-ionised water (18 MΩ cm⁻¹
resistivity; Milli-Q) and rinsed with re-distilled dichlor-
omethane (DCM). The outer edges were removed from each
sample, which was then cut into smaller pieces using a small
rock saw lubricated with water. The aim was to get fresh, un-
weathered samples for laboratory analysis. These were then
used for preparing thin sections and for stable isotope anal-
yses of carbonate veins, with 90–110 µg of calcite being
extracted using a small electrical hand drill. For organic
geochemistry selected pieces were washed with Milli-Q
water and DCM, then crushed (to particles <50 µm) using a
tungsten Tema Mill, which was previously thoroughly
cleaned (Decon-90 solution 2% v/v, milli-Q water, methanol
and finally DCM). In order to assess lab contamination,
blanks composed of pre-combusted silica gel (600 °C) were
subjected to the same procedures as the samples.

**3.3 Mineralogical analyses**

Optical microscopy, cathodoluminescence microscopy
(CL; CITL Mk5-2) and X-ray diffraction (XRD) were used
to identify the mineralogy, chemistry, texture and textural

relations within the samples and to associate the mineral-
ogy with the OM. A Panalytical X'Pert PRO XRD system
(CuK<sup>-α</sup> radiation, 45 kV, 40 mA) was used to identify the
volume proportions of the minerals associated with ser-
pentinization and the polytypes of the serpentine minerals.

**3.4 Geochemical analyses**

Analyses of total carbon (TC) and total organic carbon (TOC) were performed before and after decarbonation (10% HCL, 25 °C), respectively, using a Carlo Erba Instrument NC2500 elemental analyser.

Stable isotopic analyses of organic carbon were carried
out on five samples of four different lithologies, prepared
using a “sealed tube” method and injecting the resulting
CO₂ into a VG Sira 10 dual-inlet mass spectrometer
(Craig 1957; Frazer and Crawford 1963; Sofer 1980). The
standard error for analysis is ±0.1‰. The isotope data
was normalized to IAEA-CH7 calibration material and
reported using the VPDB scale. Carbon and oxygen iso-
tope measurements on calcite were performed on material
extracted from veins by conversion to CO₂ using a VG
SIRA 10 MS Isocarb (common acid bath). The carbon and
the oxygen isotope analyses are referenced to the VPDB
standard with a standard error for each analysis of ±0.1‰
(McCrea 1950; Craig 1957; Friedman and O'Neil 1977;
Swart et al. 1991).

Soxhlet extraction was used to extract the bitumen from
the powdered rocks using DCM: methanol (ratio 9:1)
(24 h) (modified after Wolff et al. 1995). Full blank
extractions were conducted in parallel to identify any
possible contamination. After evaporation of the solvent,
the extracts were re-dissolved and passed through short
columns of alumina and sodium sulphate using hexane as
solvent to isolate the hydrocarbons. The eluent from the
alumina column was re-dissolved in hexane (50 µL) and
analysed by gas chromatography (GC) and GC-mass
spectrometry (GC-MS).

For GC-MS we used a GC Trace 1300 and Thermoquest
ISQMS single quadrupole fitted with a split-splitless
injector, GC column (DB-5MS non-polar 5% phenyl and
95% methyl silicone stationary phase, 60 mm × 0.25 mm
i.d., film thickness 0.1 µm) using helium as a carrier gas
(2 mL min⁻¹). The GC oven temperature was programmed
from 60 to 170 °C after 1 min at 6 °C min⁻¹, then from
170 to 315 °C at 2.5 °C min⁻¹ and finally held at 315 °C
for 15 min. GC-MS was carried out in full data acquisition
mode, providing mass spectra of compounds eluting from
20 to 90 min; these were identified by comparison with the
literature and with authentic standards where available
(PAHs-polynuclear aromatic hydrocarbons). 5α(H)-Cho-
lestane was used as an external standard for quantification;
response factors were assumed to be 1, hence data are
<table><caption>**Table 2** Results by lithology including TC, TOC and total HC concentration in ppm (parts per million) and organic carbon isotopic composition of bulk rock δ¹³C<sub>organic</sub></caption><thead><tr><th rowspan="2"></th><th rowspan="2">Sample number</th><th rowspan="2">Total carbon (ppm)</th><th rowspan="2">Total organic (ppm)</th><th rowspan="2">Hydrocarbons (HC) (ppm)</th><th colspan="3">n-alkanes</th><th colspan="3">δ¹³C<sub>VpDB</sub> (%) on TOC</th><th rowspan="2">Steranes</th><th rowspan="2">Hopanes</th><th rowspan="2">PAH<sup>a</sup></th></tr><tr><th>Range</th><th>C<sub>max</sub></th><th>CPI</th><th>Mean carbon number</th></tr></thead><tbody><tr><td colspan="14"><strong>Serpentinite</strong></td></tr><tr><td>Massive serpentinite</td><td>5, 16</td><td>395-3135</td><td>102-176</td><td>23-900</td><td>16-39</td><td>C<sub>20b</sub></td><td>0.76-1.29</td><td>20-28</td><td>BDL</td><td>BDL</td><td>*</td><td>*</td></tr><tr><td>Serpentinite with numerous Ca veins</td><td>24, 26, 44, 47</td><td>20400-42500</td><td>19-135</td><td>1-124</td><td>16-35</td><td>C<sub>20b</sub></td><td>0.8-1.05</td><td>22-28</td><td>BDL</td><td>*</td><td>*</td><td>*</td></tr><tr><td>Red serpentinite (with hematite)</td><td>71</td><td>88205</td><td>268</td><td>1</td><td>17-35</td><td>C<sub>26</sub></td><td>1.17</td><td>22.3</td><td>BDL</td><td>BDL</td><td>*</td><td>**</td></tr><tr><td>Serpentinite gouge</td><td>69</td><td>539</td><td>284</td><td>3</td><td>17-33</td><td>C<sub>29</sub></td><td>0.94</td><td>26.0</td><td>BDL</td><td>*</td><td>BDL</td><td>**</td></tr><tr><td>Serpentinite cataclasite</td><td>70</td><td>876</td><td>269</td><td>46</td><td>17-36</td><td>C<sub>20</sub></td><td>0.94</td><td>24.1</td><td>BDL</td><td>BDL</td><td>**</td><td>**</td></tr><tr><td>Massive serpentinite<sup>a</sup></td><td>77, 78</td><td>454</td><td>≈190</td><td>0.05-4</td><td>17-40</td><td>C<sub>29</sub></td><td>0.84-0.97</td><td>29-30</td><td>BDL</td><td>*</td><td>BDL</td><td>**</td></tr><tr><td colspan="14"><strong>Ophicalcite</strong></td></tr><tr><td>Neptunian dykes</td><td>1, 23</td><td>95600-107500</td><td>15-103</td><td>0.03-121</td><td>17-35</td><td>C<sub>29</sub></td><td>1.05</td><td>27-29</td><td>-27.06</td><td>**</td><td>**</td><td>**</td></tr><tr><td colspan="14"><strong>Tectono-sedimentary ophicalcite</strong></td></tr><tr><td>Red matrix, serp. claste and Ca veins</td><td>21, 25, 39, 41, 42, 46, 66</td><td>33360-101700</td><td>11-116</td><td>2-2470</td><td>17-38</td><td>C<sub>18</sub> to C<sub>28</sub></td><td>0.81-1.42</td><td>23-31</td><td>-27.4</td><td>**</td><td>**</td><td>**</td></tr><tr><td>Reworked tec.-sed. ophicalcite</td><td>9</td><td>106000</td><td>86</td><td>50</td><td>16-36</td><td>C<sub>22</sub></td><td>0.65</td><td>24</td><td>-26.1</td><td>BDL</td><td>*</td><td>**</td></tr><tr><td>Serp.clasts in Ca veins</td><td>72</td><td>69600</td><td>30</td><td>124</td><td>20-36</td><td>C<sub>29</sub></td><td>0.90</td><td>28</td><td>BDL</td><td>BDL</td><td>BDL</td><td>BDL</td></tr><tr><td colspan="14"><strong>Sediments</strong></td></tr><tr><td>Radiolarite from sulphide rich outcrop</td><td>62, 68</td><td>671-13200</td><td>66-187</td><td>1-17</td><td>17-34</td><td>C<sub>20</sub>C<sub>31</sub></td><td>1.19-2.87</td><td>24-28</td><td>BDL</td><td>**</td><td>*</td><td>*</td></tr><tr><td>Siliceous shale from sulphide rich outcrop</td><td>60, 61</td><td>384-672</td><td>194-433</td><td>22-144</td><td>16-37</td><td>C<sub>20</sub>C<sub>27</sub></td><td>1.05-1.52</td><td>23-25</td><td>**</td><td>**</td><td>**</td><td>**</td></tr><tr><td>Siliceous shale near sulphide rich outcrop</td><td>15, 18</td><td>285-318</td><td>136-145</td><td>13-27</td><td>16-33</td><td>C<sub>18</sub>C<sub>20</sub></td><td>0.95-1.19</td><td>23-24</td><td>*</td><td>*</td><td>*</td><td>BDL</td></tr><tr><td>Limestones</td><td>10, 19</td><td>98200-129000</td><td>74-82</td><td>79-171</td><td>16-35</td><td>C<sub>22</sub></td><td>0.92-0.94</td><td>23-27</td><td>-26.62</td><td>BDL</td><td>***</td><td>***</td></tr><tr><td>Limestone from parsennhütte</td><td>53, 54, 56, 57, 58</td><td>46500-94500</td><td>14-92</td><td>3-238</td><td>16-37</td><td>C<sub>23</sub></td><td>0.82-1.10</td><td>22-23</td><td>*</td><td>*</td><td>*</td><td>**</td></tr><tr><td>Sulphide rich outcrop</td><td>63, 64, 65</td><td>442-90400</td><td>153-647</td><td>1-24</td><td>17-33</td><td>C<sub>20b</sub></td><td>0.93-1.55</td><td>23-26</td><td>-26.02</td><td>**</td><td>**</td><td>**</td></tr></tbody></table>

For the n-alkanes: the carbon number range (n-alkane range), the maximum carbon number (Cmax), carbon preference index (CPI values) and mean carbon number (MC#) are shown. For the steranes, hopanes and PAHs the concentrations are in ppm and are represented in intervals by star symbol *, where * = [0-0.1), ** = [0.1-1) and *** = [1-10)

BDL below detection limit, a identified PAHs in the samples were: Phenanthrene, *P* Fluoranthene, *Fluo* Pyrene, *Pyr* Chrysene, *Chry* Benzo(a)anthracene, *BaA* Benzo(b)fluoranthene, *BbF* Benzo(a)pyrene, *BaP* Indeno(1,2,3-cd)pyrene, *IndPy* Benzo(ghi)perylene, *BghiP* Dibenzothiophene (DBT), *methylP* (3,2,1 and 9 MPs) and 2-methyl naphthalene; not all were present in every sample

<footnote>a All lithology are from lower sub-unit except the massive serpentinite (samples 77, 78) which is from upper sub-unit</footnote>
semi-quantitative. Data were processed using XCalibur 1.2 software (ThermoScientific).

The mean carbon numbers, MC# (Peltzer and Gagosian 1989) and the carbon preference index (CPI) of n-alkanes (Peters et al. 2005a) were calculated over the carbon number range C20–C37 (Eqs. 5 and 6; Table 2).

$$MC\# = \sum ([C_i] \times C_i) / \sum [C_i] \qquad (5)$$

where [Cᵢ] = concentration of the n-alkane with Cᵢ carbon number

$$CPI = 0.5x \sum \frac{[\text{odd } C_{21} - C_{35}]}{\left( [\text{even } C_{20} - C_{34}] + \sum [\text{odd } C_{23} - C_{37}] / \sum [\text{even } C_{22} - C_{36}] \right)} \qquad (6)$$

## 4 Carbon and hydrocarbon distributions in the analysed lithologies

The TOC and TC results are summarized in Table 2 and vary considerably. The TOC values are low, while TC reflect carbonate contents. The stable isotopic composition of carbonate varied from −0.78 to 1.86‰VPDB and −11.7 to −6.2‰VPDB for δ¹³C and δ¹⁸O, respectively (Table 2; Fig. 4). Five decarbonated samples (sample 1: neptunian dyke; samples 9 and 39: reworked tectono-sedimentary ophicalcite; sample 19: limestone; sample 69: specimen from the sulphide-rich outcrop) have similar values for δ¹³C<sub>VPDB</sub> organic of between −27.4 and −26.2‰ (Table 2).

Hydrocarbons (HCs) identified in Totalp samples include n-alkanes, steranes, polynuclear aromatic hydrocarbons (PAHs), hopanes and isoprenoids, namely pristane and phytane. Samples of the same lithology do not necessarily have similar distributions of HCs (Table 2). This may reflect the heterogeneity of the samples collected, for

<figure><img src="image_8.png" /><figcaption>**Fig. 4** a Global range of carbon isotopic composition of carbonates precipitated during early diagenetic processes (modified after Coleman et al. 1993; Kiriakoulakis 1997; Heydari 1997). b Carbon and</figcaption></figure>

oxygen isotopic composition of calcite veins in Totalp samples. c Limestone (sample 19) showing two generations of calcite veins
a

<figure><img src="image_9.png" /></figure>

Limestone
(sample 10)

b

<figure><img src="image_10.png" /></figure>

Siliceous rock
(sample 60)

c

<figure><img src="image_11.png" /></figure>

Siliceous rock
(sample 60)
► **Fig. 5** Representative mass chromatograms of the HCs of a typical sample of limestone (sample 10) and a siliceous rock from the sulphide rich outcrop of Totalp (sample 60). **a** n-alkanes (m/z 85) of sample 10 (limestone) **b** sterane distribution (m/z 217, 218) of sample 60 **c** PAH distribution (m/z 178, 192, 202, 228) of sample 60. For abbreviations see appendix

example in the relative amount of carbonate, calcite veins and serpentinite clasts.

Aliphatic compounds (>C15), mainly in the form of *n*-alkanes dominate (Fig. 5a). The CPI (carbon preference index) for the *n*-alkanes are in the range 1 ± 0.3, except for two samples that were visibly weathered, having CPI = 2.6–2.87, i.e. an odd over even ratio for the *n*-alkanes confirming contamination from modern material, such as soil (e.g. Villanueva et al. 1997). Steranes included 20R and 20S αββ isomers from C27 to C29, as well as 20 R and S diacholestane and pseudohomologues (Fig. 5b). The steranes were dominated by C27 compounds with a lower abundance of C28 and C29 pseudo-homologues (Fig. 5b). The ranges of values for the thermal maturity parameters of the C27 steranes are between 0.41 and 0.69 for ST1 = ααα 20S/ααα20S + ααα 20R and 0.37 and 0.59 for ST2 = αββ/ααα + αββ (Fig. 6; Seifert and Moldowan 1980; Peters et al. 2005b). PAHs having molecular masses ≤276 were identified in some of the bitumen extracts (Table 2; Fig. 5c).

**4.1 Serpentinite**

All serpentinite samples contain *n*-alkanes, and several PAHs in the form of phenanthrene (P) and fluoranthene (Fluo); steranes and hopanes were largely absent (Table 2).

4.1.1 *Upper ultramafic sub-unit*

4.1.1.1 Massive serpentinite The massive serpentinites collected from the Weissfluhjoch (samples 77, 78; Fig. 1c; Table 1) area have HC concentrations between 0.05 and 4 ppm while the C₂₉ *n*-alkane dominates their distribution (Cmax = 29; Table 2). Total carbon (454 ppm) and total organic carbon (TOC) (≈180 ppm) concentrations are both very low.

4.1.2 *Lower ultramafic sub-unit*

4.1.2.1 Massive serpentinite XRD analysis shows a lizardite polytype 1 (Bailey 1969) (87%), garnet (andra-dite) (7%) and pyroxene (clinopyroxene) (5%). In thin section olivine is completely replaced by serpentine minerals, with phantoms of pyroxene, euhedral magnetite, spinel and several calcite and carbonate veins not visible to the naked eye (Table 1).

HC concentrations vary from 23 ppm for the serpen-
tinite from Parsenfurgga (sample 5) to 900 ppm from an
outcrop with ophicalcite in Obersasställi (sample 16;
Fig. 1c; Table 2). The TOC values range from 102 to
176 ppm and TC from 395 to 3134 ppm; steranes and
hopanes are below detection limits (BDL) (Table 2). Ser-
pentinite samples showing numerous calcite veins (sample
24, 26, 44 and 47) have higher amounts of TC
(20400–42500 ppm). They show variable TOC
(19–135 ppm) and low HC concentrations (1–124 ppm). In
the lower ultramafic sub-unit a red coloured serpentinite
was identified by XRD to contain hematite (4%) (Table 1).
The serpentine mineral is a lizardite polytype 1 (59%), also
containing talc (18%) and calcite (19%). The isotopic
composition of the calcite in the veins is 0.03 and

**Fig. 6** Thermal maturity parameters of C27 steranes for Totalp samples. The classification is from Peters et al. 2005a. The *brown shaded* area represents the range of complete isomerization for the 20S/(20S + 20R) ααα C27 steranes (ca. 55%) and αββ/ (ααα + αββ) C27 steranes (ca. 68%)

<figure><img src="image_12.png" /></figure>
−11.75‰ for δ¹³C<sub>VPDB</sub> and δ¹⁸O<sub>VPDB</sub>, respectively (Fig. 4).

### 4.1.3 Serpentinite cataclasite and gouges

The serpentine cataclasites and gouges have TC of 540–875 ppm, n-alkanes with a carbon number range of C₁₇–C₃₆ and no detectable steranes or hopanes (Tables 1 and 2). The serpentine cataclasites (sample 70), which are less deformed than the serpentine gouge (sample 69), contain more hydrocarbons (46 and 3 ppm, respectively Table 2) but similar TOC contents (284 and 269 ppm, respectively).

## 4.2 Ophicalcite

The ophicalcites from the Totalp unit all contain n-alkanes and the PAHs (P, Fluo and Pyr, see glossary; Table 2). Steranes and hopanes are also present in the majority of samples.

### 4.2.1 Neptunian dykes

Samples of neptunian dykes have a red micritic limestone matrix with millimetre-scale serpentinite clasts and calcite veins (samples 1, 23) (Fig. 1c; Table 1). The δ¹³C<sub>VPDB organic</sub> of one of the samples (sample 1) was −27.1‰ (Table 2). There are high amounts of TC with variable TOC (15–103 ppm) and HC concentrations (0.3–121 ppm) (Table 2).

### 4.2.2 Tectono-sedimentary ophicalcites

The tectono-sedimentary ophicalcite has various sub-lithologies from a reworked tectono-sedimentary breccia with folded serpentinized and carbonate clasts in a red carbonate matrix crosscut by calcite veins (sample 9) to centimetre scale serpentinite clasts in a carbonate vein (sample 72) (Fig. 1c; Table 1). The stable isotopic composition δ¹³C<sub>VPDB organic</sub> of one of the samples was −26.1‰ (sample 9) (Table 2). A second type of ophicalcite, a serpentinite breccia composed only of serpentinite clasts fragmented by calcite veins has low TOC (30 ppm) and HCs concentrations (124 ppm) including only n-alkanes (sample 72) (Table 2). The isotopic compositions of the calcite veins surrounding the serpentinite clast are 1.60‰ and −10.6‰ VPDB for δ¹³C and δ¹⁸O, respectively. The third most common ophicalcite is composed of anhedral serpentinite clasts, carbonate veins, calcite veinlets in a reddish pelagic matrix and has a low amount of TOC (11–116 ppm), but high concentrations of HCs (2–2470 ppm) (samples 21, 25, 39, 41, 42, 44, 66; Fig. 1c; Tables 1 and 2). The isotopic composition of the samples

varies from δ¹³C<sub>VPDB</sub> = 0.32‰, 0.42‰ and 0.58‰ with δ¹⁸O<sub>VPDB</sub> = −11.75‰, −10.28‰ and −10.9‰ (for samples 25, 42 and 39 respectively) to δ¹³C<sub>VPDB</sub> = 1.07 and 1.78‰ with δ¹⁸O<sub>VPDB</sub> = −11.7 to −7‰ for samples 42 (second generation calcite vein) and 41 (Fig. 4; Table 1). Isotopic analysis of organic carbon (sample 39) gave δ¹³C<sub>VPDB organic</sub> = −27.4‰ (Table 2). The ophicalcite outcrop near the Gotschnagrat NE Totalp has isotopic values for calcite veins of 1.86‰ for δ¹³C<sub>VPDB</sub> and −6.2‰ for δ¹⁸O<sub>VPDB</sub> (sample 66).

## 4.3 Sediments

Limestones are, together with radiolarian cherts, the most common sediments in the Totalp unit. All of the sediment samples contain detectable levels of HCs including PAHs.

### 4.3.1 Radiolarian cherts

The radiolarian cherts are situated mostly around the Gotschnagrat NE Totalp (samples 62, 68) and along small distributed outcrops some of which are 1–2 m in length and located between Parsennfurgga and Parsenhütte (sample 6; Fig. 1c). Except for the weathered sample, they have very low concentrations of HCs (1–17 ppm) and low TOC values (66–187 ppm) (Table 2). The radiolarian cherts are hard, fine grained siliceous sediments that are transected by post-depositional quartz and calcite veins, the latter having isotopic compositions of δ¹³C<sub>VPDB</sub> = −0.79‰ and δ¹⁸O<sub>VPDB</sub> = −6.4‰ (Sample 62; Table 1; Fig. 4).

### 4.3.2 Siliceous shales

The siliceous shales are located mostly above Parsennhütte and around the Gotschnagrat (samples 15, 18, 60, 61; Fig. 1c). XRD analysis of the siliceous shales (sample 61) above the pyrite rich area revealed a mineralogical composition consisting of quartz (83%), albite (10%), pyrite (2%), illite/muscovite (4%), chlorite and hematite. This composition is similar to the siliceous shale (samples 15, 18) found nearby, which consists of quartz (89%), plagioclases (6%), illite/muscovite (4%), chlorite and trace of hematite (Fig. 1c; Table 1). They have low concentrations of HCs (13–144 ppm) and variable amounts of TOC (194–433 ppm) (Table 2).

### 4.3.3 Limestone

Samples (53–57) are pelagic grey limestones that contain visible calcite veinlets and fine greyish veinlets (serpentinite) from the syncline in the Parsennhütte (Fig. 1c; Table 1). They contain a low amount of TOC (14–92 ppm) and variable concentrations of HCs (Table 2).
The pelagic limestones with higher amount of calcite veins (samples 10, 19) are also poor in TOC (74–82 ppm) and contain HCs from 79 to 171 ppm (Fig. 1c; Tables 1 and 2). Isotopic analyses of the first generation of calcite veins in sample 19 show $\delta^{13}C_{VPDB} = 0.56\text{‰}$ and $\delta^{18}O_{VPDB} = -6.5\text{‰}$ and $\delta^{13}C_{VPDB organic} = -26.6\text{‰}$ (Fig. 4; Table 1).

## 4.4 Silica-rich carbonate (sulphide-rich outcrop)

The mineralogy of the pyrite rich rocks is dominated by calcite (53%) with quartz (24%), chlorite (12%) and pyrite (6%) being the other main minerals (sample 63, 64, 65; Figs. 1c and 3; Table 1). The samples were on the top of the radiolarian chert and red shale sequences where the contact between these rocks are weathered and not affected by deformation (Fig. 3). Isotopic composition of organic carbon is $\delta^{13}C_{VPDB organic} = -26.02\text{‰}$ (sample 64) and that of the calcite veins in the same sample 1.4‰ for $\delta^{13}C_{VPDB}$ and $-6.6\text{‰}$ for $\delta^{18}O_{VPDB}$ (Table 1). The samples are characterized by a TOC contents from 153 to 647 ppm and low HC concentrations (1–24 ppm) (Table 2); bitumen extracts are

dominated by elemental sulphur, but HCs include *n*-alkanes, PAHs (P, Fluo, MPs) and steranes (Table 2).

# 5 Interpretation and discussion

Traces of OM with a composition consistent with a marine origin were found in the serpentinized exhumed mantle. The generally low and variable amounts of TOC reflects the large lithological diversity of the Totalp area, and the distributions of hydrocarbons are consistent with the temperature history of the Totalp unit (i.e. no metamorphic overprint) (Table 2; Fig. 5).

## 5.1 Source of OM

A cross-plot of Pristane (Pr) to *n*-C₁₇ versus phytane (Ph) to *n*-C₁₈ is commonly used to determine the depositional environment of OM in sedimentary rocks (Peters et al. 2005b). For the Totalp samples (Fig. 7), this cross-plot implies a reducing (anoxic) depositional environment for the OM, with a marine source of planktonic algal/bacterial OM consistent with a marine mixed transitional

<figure><img src="image_13.png" /><figcaption>**Fig. 7** Plot of pristane/*n*-C₁₇ versus phytane/*n*-C₁₈ for Totalp samples used to identify depositional environment and OM type (after Peters et al. 1999)</figcaption></figure>
**Fig. 8** Sterane ternary distribution of the analysed samples for the αββ steranes of Totalp samples. The interpretation of depositional environment is from Patrycja Wójcik-Tabol and Ślączka (2015)

<figure><img src="image_14.png" /></figure>

environment that might be expected at this fossil OCT. Sterane ternary diagrams are also commonly used to provide information on the source of OM in sedimentary rocks (Peters et al. 2005a). Comparison of Totalp steranes with predicted distributions of source materials (Fig. 8) are consistent with a mixed marine source of planktonic algal and bacterial OM (Gonçalves et al. 2013; Wójcik-Tabol and Ślączka 2015) and are similar to OM deposited in marine settings during and since the Jurassic (Grantham and Wakefield 1988; Wójcik-Tabol and Ślączka 2015). Such a source for OM in the radiolarian cherts reflects the dominant planktonic signature of coccoliths and nanoclonids (Manatschal et al. 2003). The variability in the sterane distributions most likely reflects spatial and temporal variability in environmental conditions during OM deposition.

In five samples (ophicalcite, limestone and samples from the sulphide bearing outcrop) with enough TOC to allow measurement of its stable isotopic composition, the determined values of δ¹³C<sub>VPDB organic</sub> of ca. -26.2 to -27.4‰ (Table 2) are again consistent with an origin from marine OM (-26 ± 7‰) (Schidlowski 1988; Hayes et al. 1990) and are similar to those found in hydrothermal systems from the Galapagos (δ¹³C = -27.4‰) and the Guaymas Basin (δ¹³C = -25 to -21‰) (Orem et al. 1990). Orem et al. (1990) argued that the OM in these hydrothermal systems derived from chemoautolithotrophic bacterial production. The carbon isotopic composition of methane in modern hydrothermal vents ranges from -8.8

to -19.6‰ (e.g. TAG 26°N -8/-9.5‰, Rainbow 36°14′N -15.8‰, see Charlou et al. 2002; Lost City 30°N -13.6 to -8.8‰, Kelley et al. 2005; Bradley and Summons 2010). Fixation of hydrothermal methane by methanotrophs would be expected to lead to OM more depleted in ¹³C by 15-30‰ (Summons et al. 1994; Schidlowski 2001; Templeton et al. 2006), i.e. in the range -23.8 to -34.6‰ assuming the most conservative fractionation. Hence, isotopic data from the Totalp samples suggests that there could be a contribution from methanotrophic biomass; however the values are also consistent with marine-derived OM. Taken together with the molecular data and in the absence of specific methanotrophic biomarkers (e.g. crocetane), we consider that the Totalp hydrocarbons derive from marine OM.

### 5.1.1 Thermal history of OM

The distributions of PAHs are in part consistent with a high temperature origin (e.g. Killops and Massoud 1992), for example with pyrolytic residues (e.g. Geissman et al. 1967) arising from OM alteration by hydrothermal activity (Kawka and Simoneit 1990) or low-grade metamorphism (Heymann et al. 2003). Hence, the methyl-PAH/PAH ratios of ≤0.8 observed in our samples are consistent with a pyrogenic source (Saha et al. 2009). However, the Fluo/Pyr ratio <0.6 of all the samples is lower than what would be expected of an exclusively pyrolytic source and indicates that lower temperature pathways of PAH formation also
contribute to the HCs (Fabiańska et al. 2016). The bio-
marker maturation parameters of C27 steranes fall within
values that might be expected within a temperature
envelope consistent with a moderate thermal history
(Fig. 6; ca. 80–150 °C; Mackenzie et al. 1980; Peters et al.
2005a) and are therefore consistent with the temperature
history of the basin (Früh-Green et al. 1990; Peters et al.
2005b). This suggests that where steranes are present, their
degree of isomerisation reflects gradual maturation over
time, rather than the pyrogenic process that may have
yielded PAHs at the contact of hot rock with seawater OM.

5.1.2 Origin of calcite veins

The measured carbon and oxygen isotopic composition of
carbonates are 0–2‰ and –11.7 to –6.2‰, for δ¹³C<sub>VPDB</sub>
and δ¹⁸O<sub>VPDB</sub>, respectively and are similar to values pre-
viously reported for calcite veins in ophicalcite and late
calcite veins collected in the Davos-Parsenn and Arosa
areas (Fig. 4) (Früh-Green et al. 1990). The δ¹³C<sub>VPDB</sub>
values are consistent with seawater-derived early diage-
netic calcite (Fig. 4a) (Hudson 1977; Coleman et al. 1993;
Heydari 1997). The δ¹⁸O<sub>VPDB</sub> values of the calcite veins

for the majority of samples vary between -11.7 and
-6.2‰ and indicate calcite precipitation during shallow
burial (<250 m), not influenced by organic carbon derived
CO2 but typical of Cretaceous calcite (Dix and Mullins
1992; Heydari 1997). One sample (radiolarian chert) has a
negative calcite carbon isotope value perhaps indicating the
influence of CO2 delivered from diagenesis of OM (Hey-
dari 1997).

5.1.3 OM in serpentinite–mechanism of emplacement

The occurrence of HCs in the serpentinite rocks of Totalp
is at first sight surprising. Schwarzenbach et al. (2013)
listed the five main sources of organic carbon (OC) that can
be preserved in basement rocks, namely: seawater, mantle,
Fischer–Tropsh-like reactions (F–T), in situ production
from microbial activity in the basement rock, and, ther-
mogenic decomposition of OM. The hydrocarbons recov-
ered from the Totalp serpentinites and the OC have isotopic
compositions consistent with an origin from marine OM,
i.e. from a seawater source rather than from in situ pro-
duction or abiotic F–T reactions. Therefore, the probable
origin of the OC preserved in the rocks is from dissolved

<figure><img src="image_15.png" /></figure>

**Fig. 9** Conceptual model explaining origin and migration of OM from the seawater into different lithologies in the Totalp unit. **a** The OM is represented by particulate and dissolved organic matter (POM and DOM, respectively). The OM infiltrates the basement rock by rock-fluid circulation. **b** Some OM may be deposited within the

carbonate veins (e.g. calcite veins). **c** OM circulates with fluids through fractures and porosity of sediments to migrate into the basement rock. **d** OM is preserved at the surfaces and in the interlayer surfaces of clay minerals in the sediments
and particulate organic carbon (DOC and POC) derived from seawater or thermal alteration of OM in sediments that then migrated to the basement rock within fluids (Fig. 9a). However, there could be different pathways of emplacement of OM in the serpentinites.

The OM could have been deposited within the two types of precipitated carbonate found in the serpentinite, namely the mechanically deposited carbonate that fills fractures, or via pore fluids fuelling the formation of calcite veins (Fig. 9b; Bernoulli and Weissert 1985; Früh-Green et al. 1990). The latter has an isotopic composition consistent with seawater carbonate and any DOM transported with the pore fluids would be trapped within the calcite matrix on precipitation (Figs. 4 and 9b). The presence of ophicalcite and the numerous calcite veins from the time of exhumation indicates a high supply of dissolved inorganic carbon (Ménez et al. 2012). As the OM clearly has a marine origin, it could have been emplaced by the first main phase of fluid-rock interactions at the ocean floor and the tectonosedimentary and hydrothermal processes described by Früh-Green et al. (1990). The OM may have migrated with seawater through fractures in the sediment into the basement rock (Fig. 9c; Delacour et al. 2008). These fractures formed by tectonic and crystallization stresses, which are not reliant on matrix permeability (Farough et al. 2016). Where initial contact between DOM-containing fluid and rock was at relatively high temperature, this could have led to the formation of the “pyrolytic” PAHs.

With respect to the sedimentary facies, organic carbon preservation is linked to grain size (or mineral surface area) and oxygen exposure after deposition (Fig. 9d; Hartnett et al. 1998; Kennedy et al. 2002). Clay minerals, one of the constituents of shale sediments found in the Totalp unit, strongly retain DOM both on the external surfaces and interlayer spaces of clay particles (Kennedy et al. 2002). However the Totalp sediments have low TOC values and could been highly oxidised during deposition or diagenesis. During deposition of shales, OM is also deposited as discrete biogenic particles, but largely these are not preserved; rather clays, particularly smectites, facilitate the absorption of DOM and POM from seawater and pore-fluids, and preserve it during burial (Kennedy et al. 2002).

In summary, OM in the exhumed mantle rocks at Totalp is of marine origin that migrated into the serpentinite most likely from the overlying seawater or sediment cover (Simoneit et al. 1978; Simoneit and Philp 1982). Our results are consistent with previous studies that show a lack of, or only minor formation of the CH₄ and H₂ needed for the production of OM involving serpentinization at low-temperature (McCollom and Donaldson 2016) and that the molecules necessary for life at hydrothermal systems are formed during the abiotic degradation of existing OM at low temperatures on the ocean floor (Reeves et al. 2014).

## 6 Conclusions

We provide evidence for the preservation of traces of OM originally deposited in a reducing marine environment in serpentinized mantle rocks and overlying sediments, but with no indication that the OM was generated from methanotrophic bio-systems. The presence of OM within serpentinized mantle raises two questions; how much is there and how is this OM distributed in depth? Drilling to recover rock cores from the Totalp area would allow sampling of serpentinized mantle deeper than surface outcrops and would shed light on the depth distribution of OM and its composition.

**Acknowledgements** The Margin modelling program MM3 is acknowledged for funding. A. Thompson, S. Crowley, S. Blackbird, G. Harriman, E. Fisher, J. Utley, N. Carr and S. Petev are thanked for scientific and technical assistance. Two anonymous reviewers and the editor Stefan Schmid are thanked for their helpful comments that improved the manuscript.

**Open Access** This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

## Glossary

<table><tr><td>DOM</td><td>Dissolved organic matter</td></tr><tr><td>GC</td><td>Gas chromatography</td></tr><tr><td>GC-MS</td><td>Gas chromatography-mass spectrometry</td></tr><tr><td>HC</td><td>Hydrocarbon</td></tr><tr><td>HCL</td><td>Hydrochloric acid</td></tr><tr><td>OCT</td><td>Ocean-continent transition</td></tr><tr><td>OM</td><td>Organic matter</td></tr><tr><td>PAH</td><td>Polynuclear aromatic hydrocarbons</td></tr><tr><td>P</td><td>Phenanthrene</td></tr><tr><td>Fluo</td><td>Fluoranthene</td></tr><tr><td>Pyr</td><td>Pyrene</td></tr><tr><td>Chry</td><td>Chrysene</td></tr><tr><td>BaA</td><td>Benzo(a)anthracene</td></tr><tr><td>BbF</td><td>Benzo(b)fluoranthene</td></tr><tr><td>BaP</td><td>Benzo(a)pyrene</td></tr><tr><td>IndPy</td><td>Indeno(1,2,3-cd)pyrene</td></tr><tr><td>BghiP</td><td>Benzo(ghi)perylene</td></tr><tr><td>DBT</td><td>Dibenzothiophene</td></tr><tr><td>3,2,1 and 9</td><td>3-,2-,1- and 9-methylphenanthrene</td></tr><tr><td>MPs</td><td></td></tr><tr><td>POM</td><td>Particle organic matter</td></tr><tr><td>POC</td><td>Particle organic carbon</td></tr><tr><td>PPM</td><td>Parts per million (1 ppm = 1 mg/l; 1 mg/kg)</td></tr></table>
## Steranes

<table><tr><td>C27βα20S</td><td>20S 13β(H),17α(H)-diacholestane</td></tr><tr><td>C27βα20R</td><td>20R 13β(H),17α(H)-diacholestane</td></tr><tr><td>C27αβ20S</td><td>20S 13β(H),17α(H)-diacholestane</td></tr><tr><td>C27αβ20R</td><td>20S 13β(H),17α(H)-diacholestane</td></tr><tr><td>C28βα20S</td><td>20S 24-methyl-13β(H),17α(H)-diacholestane</td></tr><tr><td>C28βα20R</td><td>20R 24-methyl-13β(H),17α(H)-diacholestane</td></tr><tr><td>C27ααα20S</td><td>20S 5α(H), 14α(H), 17α(H)-cholestane</td></tr><tr><td>C27αββ20R</td><td>20R 5α(H), 14α(H), 17α(H)-cholestane</td></tr><tr><td>C27αββ20S</td><td>20S 5α(H), 14α(H), 17α(H)-cholestane</td></tr><tr><td>C27ααα20R</td><td>20R 5α(H), 14α(H), 17α(H)-cholestane</td></tr><tr><td>C28ααα20S</td><td>20S 24-methyl-5α(H), 14α(H), 17α(H)-cholestane</td></tr><tr><td>C28αββ20R</td><td>20R 24-methyl-5α(H), 14α(H), 17α(H)-cholestane</td></tr><tr><td>C28αββ20S</td><td>20S 24-methyl-5α(H), 14α(H), 17α(H)-cholestane</td></tr><tr><td>C28ααα20R</td><td>20R 24-methyl-5α(H), 14α(H), 17α(H)-cholestane</td></tr><tr><td>C29ααα20S</td><td>20S 24-ethyl-5α(H), 14α(H), 17α(H)-cholestane</td></tr><tr><td>C29αββ20R</td><td>20R 24-ethyl-5α(H), 14α(H), 17α(H)-cholestane</td></tr><tr><td>C29αββ20S</td><td>20S 24-ethyl-5α(H), 14α(H), 17α(H)-cholestane</td></tr><tr><td>C29ααα20R</td><td>20R 24-ethyl-5α(H), 14α(H), 17α(H)-cholestane</td></tr><tr><td>TOC</td><td>Total organic matter</td></tr><tr><td>TC</td><td>Total carbon</td></tr><tr><td>XRD</td><td>X-ray diffraction</td></tr><tr><td>‰</td><td>Permille symbol</td></tr></table>

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