# Genomic characterization of Listeria spp. isolated from tonsils, udder and feces of domestic dairy ruminants in Spain

Carla Palacios-Gorba, Alexandra Moura, Yuval Markovich, Nathalie Tessaud-Rita, Ángel Gómez-Martín, Hélène Bracq-Dieye, Jesús Gomis, Guillaume Vales, María Pastor-Martín, Pierre Thouvenot, et al.

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## ► To cite this version:

Carla Palacios-Gorba, Alexandra Moura, Yuval Markovich, Nathalie Tessaud-Rita, Ángel Gómez-Martín, et al. Genomic characterization of Listeria spp. isolated from tonsils, udder and feces of domestic dairy ruminants in Spain. Microbes and Infection, 2022, pp.105079. 10.1016/j.micinf.2022.105079. pasteur-03922761

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<figure><img src="image_2.png" /></figure>
Genomic characterization of *Listeria* spp. isolated from tonsils, udder, and feces
of domestic dairy ruminants in Spain

Carla Palacios-Gorbaª, Alexandra Mouraᵇ,ᶜ, Yuval Markovichª, Nathalie
Tessaud-Ritaᵇ,ᶜ, Ángel Gómez-Martínᵈ, Hélène Bracq-Dieyeᵇ,ᶜ, Jesús Gomisᵈ,
Guillaume Valesᵇ,ᶜ, María Pastor-Martínᵉ, Pierre Thouvenotᵇ,ᶜ, Carles Escrigf,
Alexandre Leclercqᵇ,ᶜ, Marc Lecuitᵇ,ᶜ,ghi, Juan J. Queredaª*

<footnote>ª Research Group Intracellular Pathogens: Biology and Infection, Departamento
Producción y Sanidad Animal, Salud Pública Veterinaria y Ciencia y Tecnología
de los Alimentos, Facultad de Veterinaria, Universidad Cardenal Herrera-CEU,
CEU Universities, Valencia, Spain.</footnote>

<footnote>ᵇ Institut Pasteur, National Reference Centre and WHO Collaborating Centre for
*Listeria*, Paris, France.</footnote>

<footnote>ᶜ Institut Pasteur, Université Paris Cité, Inserm U1117, Biology of Infection Unit,
Paris, 75015, France.</footnote>

<footnote>ᵈ Research Group Microbiological Agents Associated with Animal Reproduction
(ProVaginBIO), Departamento Producción y Sanidad Animal, Salud Pública
Veterinaria y Ciencia y Tecnología de los Alimentos, Facultad de Veterinaria,
Universidad Cardenal Herrera-CEU, CEU Universities, Valencia, Spain.</footnote>

<footnote>ᵉ Departamento de Salud de Manises, Conselleria de Sanitat Universal i Salut Pública, Generalitat Valenciana, Valencia, Spain.</footnote>

<footnote>f Public Health Center, Castellón, Dirección General de Salud Pública y
Adicciones. Consellería de Sanidad Universal y Salud Pública. Comunidad
Valenciana, Spain.</footnote>
⁹ Necker-Enfants Malades University Hospital, Division of Infectious Diseases and Tropical Medicine, Institut Imagine, APHP, Paris, France.

Running title: *Listeria* spp. from ruminants' organs

* To whom correspondence should be addressed: juan.quereda@uchceu.es
C/ Tirant lo Blanc, 7. 46115 Valencia, Spain. Tel. 96 136 90 00 - 66011
Abstract

Two species of *Listeria* are pathogenic, *Listeria monocytogenes* and *Listeria ivanovii*. Although studies have shown that dairy ruminants shed *Listeria* spp. in feces, there is little information about ruminants that do not shed *Listeria* spp. in their feces but asymptomatically carry them in organs. We evidence that ruminants can asymptomatically carry *L. ivanovii* in udders and *L. monocytogenes* and *L. ivanovii* in tonsils without fecal shedding. Whole-genome sequence of *L. monocytogenes* and *L. ivanovii* contained known core genes involved in virulence and antibiotic resistance. This work highlights tonsils and udders as a Listeria intra-host site of colonization.

Keywords: *Listeria* spp.; dairy; ruminant; tonsil; udder; WGS
# 1. Introduction

Listeriosis is recognized as a significant public health issue due to the severity of the disease and its mortality rate (20% to 30%) [1,2]. Only two species of the genus *Listeria*, *Listeria monocytogenes* (*Lm*) and *Listeria ivanovii* (*Liv*), are considered facultative intracellular pathogens [2]. *Lm* can cause serious invasive disease (including central nervous system and fetal-placental infection) in animals and humans [1,2]. Although *Liv* is mainly an animal pathogen, its isolation from human patients with listeriosis symptoms has been reported [3]. *Listeria innocua* (*Lin*) is a close relative of *Lm* and is nonpathogenic to mammals, although atypical virulent isolates have been reported [4].

Healthy people and domestic ruminants tend to be asymptomatic carriers that shed *Lm*, *Liv* and non-pathogenic *Listeria* spp. in their feces [5,6]. Fecal shedding of *Lm* could lead to contamination of agricultural environments and raw produce which are then transported to food processing industries [7]. In dairy farms, *Lm* is usually transmitted to bulk tank milk as an outcome of environmental or fecal contamination of the udder [8].

While the incidence of listeriosis in humans is low compared with other foodborne infections, listeriosis is recognized as a significant public health issue due to its severity and high mortality rate [1]. *Lm* population is diverse, comprising 4 lineages (ref), and its population structure can be determined by using multilocus sequence typing (MLST) and core genome MLST (cgMLST). MLST, which analyzes seven housekeeping genes, groups *Lm* isolates in sequence types (STs) and clonal complexes (CCs or clones) (Ref Ragon2008). cgMLST, which analyzes 1748 core loci, allows *Lm* classification in cgMLST types (CTs) and
sublineages (SLs) [9]. *Listeria* spp. surveillance in animal production settings normally targets fecal or environmental samples, however, information on *Listeria* spp. presence in organs such as tonsils and udders is still scarce. Moreover, there is no information about asymptomatic carriage of *Listeria* spp. in ruminants' organs without fecal shedding. Here, we used whole-genome sequencing to subtype and characterize *Listeria* spp. isolated from feces, tonsils, and udders from domestic dairy ruminants. Increasing the understanding of the reservoirs and genomic characteristics of *Lm* and *Liv* will help to improve their control, enhancing human and animal disease prevention.

## 2. Materials and methods

### 2.1 Animals and sampling

In the time frame of December 2019 to May 2021, 93 dairy sheep (*Ovis orientalis* aries breed Lacaune, Manchega, Assaf, and crossbreed), 10 dairy goats (*Capra aegagrus hircus* breed Guadarrama and crossbreed), and 183 dairy cattle (*Bos primigenius taurus* breed Holstein-Friesian) were slaughtered in two slaughterhouses (slaughterhouse A located in Madrid for small ruminants and slaughterhouse B located in Valencia for cattle). Each slaughtering was followed by a systematic cleaning and disinfection protocol. Veterinary supervision was provided throughout the slaughter and Good Manufacturing Practices (GMP), Sanitation Standard Operating Procedures (SSOP), Hazard Analysis Critical Control Point (HACCP), and the traceability was strictly followed. Additionally, 29 dairy sheep and 1 dairy goat were culled for educational purposes to the authors' university. An intravenous injection of barbiturate was used to euthanize these additional animals, and complete necropsies were carried out immediately
following death. Sheep and goats originated from 36 farms located in the provinces (administrative division in Spain) from Valencia, Albacete, Cuenca, Toledo, Ciudad-Real, and Madrid (Table S1). Cattle originated from 13 farms located in the provinces of Murcia, Albacete, Teruel, Alicante and Valencia (Table S1 and Fig. S2). Approximately 10 g of tonsils and udders were removed aseptically and stored in sterile bags. Tonsils were sampled while the carcasses were hanging upside down which implies that surface contamination was not possible. Udders were sampled once they were removed from the animal and located on a tray. A sample from the inner part of the udder was obtained by applying a cut on a previous decontaminated part of the organ surface with alcohol. Veterinary inspectors assumed that slaughtered animals were healthy based on the lack of abnormal behavior, clinical signs and macroscopic abnormalities in the thoracic and abdominal cavities of the carcasses. Thoracic and abdominal organs were inspected to evaluate position, size, and color. Serosal surfaces were examined, and organs evaluated for any grossly visible abnormalities in structure and/or pathologic processes. The mandibular, the lung (cervical, mediastinal, tracheobronchial) and mesenteric lymph nodes, were also identified and evaluated. Collected feces from the rectum of each ruminant were stored in independent sterile bags. Each animal sample (tonsils, udder, and rectum) was handled with a new and independent sterile scalpel to prevent contamination. All samples were collected using disposable gloves under aseptic conditions. Within 24h, the entire sample material was transported to the laboratory at 5+/-3°C.

## 2.2 *Listeria* species isolation and identification.
*Listeria* spp. were isolated as previously described [6,10,11]. Briefly, 8 g of rectal fecal samples, 5 g of tonsils, and udders were disrupted and diluted 1/10 in Half-Fraser broth (Scharlab, Spain). An enrichment step of 24 h at 30 °C was performed on homogenized samples. Incubated suspensions were transferred into Fraser broth (Scharlab, Spain), and incubated for 24 h at 37 °C, before being transferred to RAPID'L. mono plates (BioRad, USA) and incubated at 37 °C for 24 h. RAPID'L. mono plates support growth of *L. monocytogenes* (PIPLC+/xylose-, blue colonies), *L. ivanovii* (PIPLC+/xylose+,blue-green colonies with yellow halo), and other *Listeria* spp. (white colonies). When more than one type of colony was present in RAPID'L.mono plates, one blue (no plate contained simultaneously *L. monocytogenes* and *L. ivanovii*) and one white *Listeria* spp. were picked and further examined in selective Oxford agar plates for *Listeria* (Scharlab, Spain). From each positive sample, one isolate colony was obtained (if in the same plate both pathogenic and non-pathogenic *Listeria* spp. were present, one colony of each was selected) and sent to the World Health Organization Collaborating Center *Listeria* (Institut Pasteur, Paris) for characterization. As described previously, *Listeria* isolates were identified using matrix-assisted laser desorption ionization-time of flight mass spectrometry applying the MicroFlex LT system with the last MBT library DB-7854 (Bruker Daltonics, Germany) [12] and by whole genome sequencing [10].

## 2.3 DNA extraction, genome sequencing and sequence analysis.

DNA was purified with the NucleoSpin Tissue DNA kit (Machenry-Nagel)
from 900 µL bacterial cultures grown overnight in 5 mL of brain heart infusion broth (Becton Dickinson, USA) at 37 °C.

DNA libraries were prepared with Nextera XT DNA Sample kit (Illumina, USA), following manufacturer's protocol, and run in NextSeq 500 platform (Illumina, USA) to obtain paired-end reads (2 x 150 bp run). Raw reads were trimmed with fqCleaner v.21.10 and assembled with SPAdes v.3.15.0, using automatic k-mers selection and the --only-assembler and --careful parameters.

In silico typing was performed from the assemblies using BIGSdb-Listeria v.1.33.1 [13] using the genoserogrouping, MLST, cgMLST profiles, resistance and virulence schemes implemented within [9], as previously described [6,9]. A maximum phylogeny was inferred from the core genome alignment built using Parsnp, v1.5.6 (as implemented in Harvest suite 1.3 [14]), using IQ-TREE/2.0.6 [15]. Trees were visualized with iTol v.4.2 [16].

## 2.4 Statistical analysis

IBM SPSS Statistics version 25 was used for all statistical analyses. Statistical tests were considered significant at P<0.05. Chi-square (χ²) tests were performed to determine the effect of geographical location of farms and animal species on the number of *Lm* or *Liv* carriage.

## 2.5 Data availability.

All sequences are publicly available at the European Nucleotide Archive (BioProject PRJEB51772) and BIGSdb-Listeria (https://bigsdb.pasteur.fr/listeria).
### 3. Results and discussion

A total of 948 tonsil, udder, and feces samples were collected from 316 dairy ruminants (183 cows, 122 sheep, and 11 goats) in two slaughterhouses and one necropsy room over 18 months. Three different *Listeria* spp. were detected in 35 animals (Table 1 and Table S1). *Lm* was isolated from 4 (1.3%) and 2 (0.6%) out of 316 animal tonsils, and feces samples, respectively (Table 1 and Table S1). *Liv* was isolated from 2 (0.6%), 1 (0.3%), and 13 (4.1%) out of 316 animal tonsils, feces, and udder samples, respectively (Table 1 and Table S1). *Lin* was isolated from 15 (4.7%) out of 316 feces samples but was not detected in any of the tonsils or udder samples (Table 1 and Table S1). Co-occurrence of different *Listeria* spp. (*Lm* and *Lin*) was detected in 0.6% (2/316) of individual animal feces (Fig. 1, Fig. S1, Table S1). *Lm* and *Liv* were detected more frequently in cattle (19/183 positive cows, prevalence 10.4%) than in small ruminants (2/122 positive sheep, prevalence 1.6%, and 0/11 positive goats, 0%) (χ² test, P<0.05), in agreement with our previous longitudinal study performed in 19 dairy ruminant farms [6]. Regarding, non-pathogenic *Listeria* spp. the prevalence was significantly higher in small ruminants than in cattle (χ² test, P<0.05), as previously shown [6]. These results show that dairy ruminants can silently carry *Lm* or *Liv* in udders or tonsils even without fecal shedding, as we previously reported in wild animals [11]. Other studies have shown that large numbers of *Lm* can be isolated in the tonsils of experimental orally infected sheep despite fecal excretion stopped [17], suggesting that tonsils are a location of *Lm* colonization. *Lm* is conveyed to milk through contamination of the udder surface or the equipment used for milking [8]. *Lm* is infrequently isolated as a cause of dairy ruminant mastitis, which tend to be
asymptomatic [18,19]. The rarity of listerial mastitis cases support the hypothesis that most of the *Listeria* milk contamination cases may arise from the udder surface or the milking equipment.

*Liv* positive cows originated more frequently from farms situated in the province of Murcia (14/82, prevalence 17.1%) than in the province of Valencia (2/88, prevalence 2.2%) (P<0.05) (Fig. S2). According to official statistics, the province of Murcia has 77.346 bovines in a surface of 11.313 km², whereas the province of Valencia has 25.664 bovines in a surface of 10.841,82 km² suggesting that *Liv* prevalence could be influenced by animal density. However, since the number of animals that were slaughtered was not homogeneous across farms (Fig. S2), it could not be determined if the differences in the prevalence of *Liv* observed between Valencia and Murcia provinces could be attributed to a farm or a province effect. Previous environmental studies have shown that the occurrence of *Liv* is rare compared with *Lm* [20]. Despite the less frequent isolation of *Liv*, this species has been found in human feces, domestic ruminant feces and milk, farm environments, dairy processing facilities, and wild boar and deer tonsils [2, 5, 21, 22]. In line with our results showing the presence of *Liv* in cow udders, a survey to evaluate the occurrence of *Liv* in food processing environments and foods in the Republic of Ireland, showed that a higher prevalence was detected for the dairy sector compared to the meat, seafood, and fresh-cut vegetable sectors (1.7 vs 0.2, 0.3, and 0%, respectively) [21]. Since a two-step enrichment protocol was used to enhance detection of *Listeria* spp., and the culled cows did not show mastitis clinical signs in our study, it is plausible that the number of *Liv* in the analyzed udders was low. The probable low numbers of *Liv* in the udders together with a restricted geographical distribution of *Liv* could explain why this
species is not commonly detected during surveillance of milk products and only
few reports describe its higher prevalence in the milk sector compared to other
food sectors [21]. Interestingly, *Liv* isolates obtained from varied geographical
and temporal sources in the present study were mainly associated with udders.

Genome-based genotyping results indicated that *Lm* isolates belonged to CC9 (*n*=2), CC4 (*n*=1), CC6 (*n*=1), CC37 (*n*=1), and CC388 (*n*=1) (Table S1, Fig. 1 and Fig. S1). CC9 was previously reported as a CC associated with meat products [23]. CC4 and CC37 were previously reported as CCs associated with dairy products [23]. Importantly, CC4, CC6 and CC388 belong to lineage I which is significantly associated with a clinical origin both in cattle and humans [24].

CC4 and CC6 are more invasive and colonize better the intestinal lumen [23].

Moreover, CC4 and CC6 have been associated with human clinical cases some
even without immunosuppressive conditions [24]. This and previous studies
suggest that the population of *Liv* is genetically less diverse than *Lm* (Fig. S1)
[11,25]. The significance of these observations and their relation to host
adaptation will require further research.

The core virulence genes of LIPI-1 (necessary for the intracellular survival) (the
term LIPI stands for *Listeria pathogenicity island*) and the *inlAB* locus (necessary
for bacterial internalization into eukaryotic cells) were present in all *Lm* and *Liv*
isolates (Fig. 1). *Listeria pathogenic island 3* (LIPI-3), which promotes intestinal
colonization by *Lm* [2], was present in 33.3% (2/6) *Lm* and 53.3% (8/15) *Lin*.
*Listeria pathogenic island 4* (LIPI-4), which increases *Lm* neural and placental
tropism [24], was present in 33.3% (2/6) *Lm* and 93.3% (14/15) *Lin* (Fig. 1). LIPI-
2, involved in virulence [2], was present in 100% (16/16) *Liv* (Fig. 1). Autolysins
(*aut*, *aut_IVb*), and LPxTG proteins coding genes (*inlE*, *inlG*, *inlH*, *inlJ*, *inlK* and
vip) related to invasion were present in *Lm* (Fig. 1, Table S2). Other genes important for teichoic acids biosynthesis, adherence, regulation, and biofilm formation were present in *Lm*, *Liv* and *Lin* (Table S2). Intrinsic antibiotic resistance genes (*fosX*, *lin* (Imo0919), *norB*, and *sul*) were present in all *Lm*, *Liv* and *Lin* isolates. *tetM* and *InuG* (resistance to tetracyclines and lincosamides) genes were also present in *Lin* (5/15, 33.3%; 1/15, 6.7%, respectively). The SSI-1 stress survival islet, involved in *Lm* growth in high salt concentrations and low pH, was present in 2 out of 3 lineage II *Lm* isolates. The SSI-2 stress survival islet, which contributes to the alkaline and oxidative stress responses, was present in 14 out of 15 *Lin* isolates (Fig. 1).

The results of this study contributes to the understanding of the pathogenic potential and ecology of *Listeria* spp. in dairy ruminants’ organs. We have revealed that (i) tonsils and udders constitute a reservoir of *Lm* or *Liv* in domestic dairy ruminants; (ii) dairy ruminants can silently carry *Lm* or *Liv* in udders or tonsils even without fecal shedding; and (iii) *Lm* and *Liv* are detected more frequently in cattle than in small ruminants. Dairy ruminants may contaminate milk or crops and the environment which can pass *Lm* and *Liv* to humans via food. The present discoveries could help public health authorities to better control the health risks associated with *Lm* and *Liv*.

## Acknowledgments

This work was supported by Generalitat Valenciana (Project reference AICO/2021/278) (JJQ), the Spanish Ministry of Science and Innovation (Project references PID2019-110764RA-I00/AEI/10.13039/501100011033 (JJQ) and
PID2020-119462RA-I00/AEI/10.13039/501100011033 (AGM)) and Universidad CEU Cardenal Herrera Programa INDI 20/40 (JJQ), Institut Pasteur, Inserm, and Santé Publique France (ML). J.J. Quereda is supported by a "Ramón y Cajal" contract of the Spanish Ministry of Science, Innovation, and Universities (RYC-2018-024985-I). C. Palacios-Gorba and Yuval Markovich are supported by a Predoctoral contract from the Universidad Cardenal Herrera-CEU. The funders had no role in study design, data collection, and interpretation, or the decision to submit the work for publication. We thank the collaboration of Juan M. Corpa who contributed to the sampling of some of the studied sheep and goat in the necropsy room of the Veterinary Faculty.

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## Figure legends

Fig 1. Genomic diversity of *Listeria* spp. found in this study (N=37). A) Midpoint rooted maximum likelihood phylogeny (GTR+F+I+G4 model, 1000 ultrafast bootstraps) inferred based on the core SNP alignment (327,775 nucleotides) using *L. ivanovii* *subsp. ivanovii* CLIP 2021/00402 arbitrary reference. *Listeria* species are shown in different colors. Information on the isolate's name, origin, date of isolation, CT, and SL are provided. Bold names highlight isolates from the same animal. B) resistance and virulence gene patterns. Color-filled boxes represent the presence of different genetic traits.

## Supplementary Figures

Fig S1. Unrooted core genome phylogenies of *Listeria* spp. found in this study. A) *L. monocytogenes* (2,506,493 nucleotides alignment). B) *L. ivanovii_subsp. ivanovii* (2,791,218 nucleotides alignment). C) *L. innocua* (2,446,398 nucleotides alignment). Tips are colored by source type and sample origin. Dashed circles delimitate clonal complexes (CCs). Isolates obtained from the same animal are marked with a #.

Fig S2. Geographic map of Spain provinces and the *Liv* prevalence per cattle farm.
<figure><img src="image_3.png" /><figcaption>Figure 1</figcaption></figure>
<figure><img src="image_4.png" /><figcaption>Supplementary figure 1</figcaption></figure>

A *Listeria monocytogenes*

B *Listeria ivanovii* subsp. *ivanovii*

C *Listeria innocua*
<figure><img src="image_5.png" /><figcaption>Supplementary figure 2</figcaption></figure>
<table><caption>Table 1. Number of isolates obtained in this study (N =37).</caption><thead><tr><th>Listeria spp.</th><th>Cow feces (n=183)</th><th>Cow tonsils (n=183)</th><th>Cow udder (n=183)</th><th>Sheep feces (n=122)</th><th>Sheep tonsils (n=122)</th><th>Sheep udder (n=122)</th><th>Goat feces (n=11)</th><th>Goat tonsils (n=11)</th><th>Goat udder (n=11)</th></tr></thead><tbody><tr><td>L. monocytogenes</td><td>1 (0.5%)</td><td>3 (1.6%)</td><td>0</td><td>1 (0.8%)</td><td>1 (0.8%)</td><td>0</td><td>0</td><td>0</td><td>0</td></tr><tr><td>L. ivanovii subsp. ivanovii</td><td>1 (0.5%)</td><td>2 (1.1%)</td><td>13 (7.1%)</td><td>0 (%)</td><td>0 (0%)</td><td>0 (0%)</td><td>0</td><td>0</td><td>0</td></tr><tr><td>L. innocua</td><td>3 (1.6%)</td><td>0</td><td>0</td><td>9 (7.4%)</td><td>0</td><td>0</td><td>3 (27.2%)</td><td>0</td><td>0</td></tr></tbody></table>
<table><caption>Table S1. *Listeria* isolates characterized in this study.</caption><thead><tr><th>BIGSdb id</th><th>isolate</th><th>aliases</th><th>species</th><th>isolation date</th><th>province</th><th>farm</th><th>source</th><th>source details</th><th>no. contigs</th><th>total length (bp)</th><th>NS0 contig length (bp)</th><th>G+C content (%)</th><th>cgMLST loci called (%)</th><th>phylogenetic lineage</th><th>genos器group</th><th>CC (MLST)</th><th>ST (MLST)</th><th>SL (cgMLST)</th><th>CT (cgMLST)</th></tr></thead><tbody><tr><td>77387</td><td>CLIP 2021/00361</td><td>LISVAL 368</td><td>L. innocua</td><td>2019-12-01</td><td>Toledo</td><td>Farm 16</td><td>Animal 8</td><td>sheep - feces</td><td>26</td><td>2893822</td><td>579505</td><td>37.3</td><td>n.a.</td><td>n.a.</td><td>L</td><td>CC493</td><td>ST493</td><td>n.a.</td><td>n.a.</td></tr><tr><td>77388</td><td>CLIP 2021/00362</td><td>LISVAL 369</td><td>L. innocua</td><td>2019-12-01</td><td>Toledo</td><td>Farm 16</td><td>Animal 7</td><td>sheep - feces</td><td>31</td><td>2894284</td><td>566880</td><td>37.3</td><td>n.a.</td><td>n.a.</td><td>L</td><td>CC493</td><td>ST493</td><td>n.a.</td><td>n.a.</td></tr><tr><td>77389</td><td>CLIP 2021/00363</td><td>LISVAL 370</td><td>L. innocua</td><td>2019-12-01</td><td>Toledo</td><td>Farm 16</td><td>Animal 4</td><td>sheep - feces</td><td>26</td><td>2894491</td><td>483337</td><td>37.3</td><td>n.a.</td><td>n.a.</td><td>L</td><td>CC493</td><td>ST493</td><td>n.a.</td><td>n.a.</td></tr><tr><td>77390</td><td>CLIP 2021/00364</td><td>LISVAL 371</td><td>L. innocua</td><td>2019-12-01</td><td>Toledo</td><td>Farm 16</td><td>Animal 6</td><td>sheep - feces</td><td>31</td><td>2894949</td><td>270613</td><td>37.3</td><td>n.a.</td><td>n.a.</td><td>L</td><td>CC493</td><td>ST493</td><td>n.a.</td><td>n.a.</td></tr><tr><td>77391</td><td>CLIP 2021/00365</td><td>LISVAL 376</td><td>L. innocua</td><td>2019-12-01</td><td>Cuenca</td><td>Farm 2</td><td>Animal 34</td><td>sheep - feces</td><td>17</td><td>2871184</td><td>543767</td><td>37.3</td><td>n.a.</td><td>n.a.</td><td>L</td><td>CC1480</td><td>ST1480</td><td>n.a.</td><td>n.a.</td></tr><tr><td>77392</td><td>CLIP 2021/00366</td><td>LISVAL 377</td><td>L. innocua</td><td>2019-12-01</td><td>Cuenca</td><td>Farm 3</td><td>Animal 39</td><td>sheep - feces</td><td>20</td><td>2784629</td><td>479655</td><td>37.4</td><td>n.a.</td><td>n.a.</td><td>L</td><td>CC599</td><td>ST2753</td><td>n.a.</td><td>n.a.</td></tr><tr><td>77422</td><td>CLIP 2021/00378</td><td>LISVAL 454</td><td>L. innocua</td><td>2020-02-13</td><td>Murcia</td><td>Farm 7</td><td>Animal 46</td><td>cow - feces</td><td>95</td><td>2822874</td><td>100830</td><td>37.4</td><td>n.a.</td><td>n.a.</td><td>L</td><td>CC474</td><td>ST474</td><td>n.a.</td><td>n.a.</td></tr><tr><td>77425</td><td>CLIP 2021/00381</td><td>LISVAL 457</td><td>L. innocua</td><td>2020-02-13</td><td>Cuenca</td><td>Farm 8</td><td>Animal 67</td><td>sheep - feces</td><td>67</td><td>2871346</td><td>144067</td><td>37.4</td><td>n.a.</td><td>n.a.</td><td>L</td><td>CC530</td><td>ST530</td><td>n.a.</td><td>n.a.</td></tr><tr><td>77426</td><td>CLIP 2021/00382</td><td>LISVAL 544</td><td>L. innocua</td><td>2020-02-28</td><td>Ciudad Real</td><td>Farm 9</td><td>Animal 5</td><td>goat - feces</td><td>46</td><td>2847186</td><td>140717</td><td>37.4</td><td>n.a.</td><td>n.a.</td><td>L</td><td>CC530</td><td>ST530</td><td>n.a.</td><td>n.a.</td></tr><tr><td>77427</td><td>CLIP 2021/00383</td><td>LISVAL 545</td><td>L. innocua</td><td>2020-02-28</td><td>Ciudad Real</td><td>Farm 9</td><td>Animal 3</td><td>goat - feces</td><td>45</td><td>2846589</td><td>188483</td><td>37.4</td><td>n.a.</td><td>n.a.</td><td>L</td><td>CC530</td><td>ST530</td><td>n.a.</td><td>n.a.</td></tr><tr><td>77428</td><td>CLIP 2021/00384</td><td>LISVAL 546</td><td>L. innocua</td><td>2020-02-28</td><td>Ciudad Real</td><td>Farm 9</td><td>Animal 9</td><td>goat - feces</td><td>48</td><td>2846234</td><td>219660</td><td>37.4</td><td>n.a.</td><td>n.a.</td><td>L</td><td>CC530</td><td>ST530</td><td>n.a.</td><td>n.a.</td></tr><tr><td>77432</td><td>CLIP 2021/00388</td><td>LISVAL 615</td><td>L. innocua</td><td>2020-06-18</td><td>Valencia</td><td>Farm 15</td><td>Animal 94</td><td>cow - feces</td><td>28</td><td>2971457</td><td>333301</td><td>37.3</td><td>n.a.</td><td>n.a.</td><td>L</td><td>CC2754</td><td>ST2754</td><td>n.a.</td><td>n.a.</td></tr><tr><td>79916</td><td>CLIP 2021/01137</td><td>LISVAL 772</td><td>L. innocua</td><td>2021-05-10</td><td>Castellón</td><td>Farm 13</td><td>Animal 119</td><td>sheep - feces</td><td>38</td><td>2815802</td><td>138611</td><td>37.3</td><td>n.a.</td><td>n.a.</td><td>L</td><td>CC1616</td><td>ST1616</td><td>n.a.</td><td>n.a.</td></tr><tr><td>79917</td><td>CLIP 2021/01138</td><td>LISVAL 773</td><td>L. innocua</td><td>2021-05-10</td><td>Castellón</td><td>Farm 13</td><td>Animal 120</td><td>sheep - feces</td><td>46</td><td>2864904</td><td>210079</td><td>37.3</td><td>n.a.</td><td>n.a.</td><td>L</td><td>CC1480</td><td>ST1480</td><td>n.a.</td><td>n.a.</td></tr><tr><td>79935</td><td>CLIP 2021/01149</td><td>LISVAL 784</td><td>L. innocua</td><td>2021-05-20</td><td>Valencia</td><td>Farm 14</td><td>Animal 183</td><td>cow - feces</td><td>13</td><td>2857362</td><td>459131</td><td>37.5</td><td>n.a.</td><td>n.a.</td><td>L</td><td>CC1482</td><td>ST1482</td><td>n.a.</td><td>n.a.</td></tr><tr><td>77393</td><td>CLIP 2021/00367</td><td>LISVAL 408</td><td>L. monocytogenes</td><td>2020-01-16</td><td>Murcia</td><td>Farm 4</td><td>Animal 11</td><td>cow - tonsil</td><td>35</td><td>3000468</td><td>477704</td><td>37.8</td><td>100</td><td>II</td><td>IIc</td><td>CC9</td><td>ST9</td><td>CT9007</td><td>CT9007</td></tr><tr><td>77394</td><td>CLIP 2021/00368</td><td>LISVAL 409</td><td>L. monocytogenes</td><td>2020-01-16</td><td>Murcia</td><td>Farm 5</td><td>Animal 13</td><td>cow - tonsil</td><td>84</td><td>3003383</td><td>192819</td><td>37.8</td><td>99.6</td><td>II</td><td>IIc</td><td>CC9</td><td>ST9</td><td>CT9007</td><td>CT9007</td></tr><tr><td>77395</td><td>CLIP 2021/00369</td><td>LISVAL 410</td><td>L. monocytogenes</td><td>2020-01-16</td><td>Murcia</td><td>Farm 4</td><td>Animal 26</td><td>cow - tonsil</td><td>37</td><td>2887721</td><td>501910</td><td>38</td><td>99.9</td><td>I</td><td>IVb</td><td>CC4</td><td>ST4</td><td>SL4</td><td>CT9158</td></tr><tr><td>77421</td><td>CLIP 2021/00377</td><td>LISVAL 453</td><td>L. monocytogenes</td><td>2020-02-13</td><td>Murcia</td><td>Farm 7</td><td>Animal 46</td><td>cow - feces</td><td>67</td><td>2991961</td><td>150603</td><td>37.9</td><td>99.9</td><td>II</td><td>IIa</td><td>CC37</td><td>ST37</td><td>SL37</td><td>CT1618</td></tr><tr><td>79286</td><td>CLIP 2021/01103</td><td>LISVAL 738</td><td>L. monocytogenes</td><td>2021-04-29</td><td>Castellón</td><td>Farm 13</td><td>Animal 112</td><td>sheep - tonsil</td><td>43</td><td>2926934</td><td>292321</td><td>37.9</td><td>99.9</td><td>I</td><td>IVb</td><td>CC6</td><td>ST6</td><td>SL6</td><td>CT9763</td></tr><tr><td>79918</td><td>CLIP 2021/01139</td><td>LISVAL 774</td><td>L. monocytogenes</td><td>2021-05-10</td><td>Castellón</td><td>Farm 13</td><td>Animal 120</td><td>sheep - feces</td><td>112</td><td>2927659</td><td>70512</td><td>37.9</td><td>99.7</td><td>I</td><td>IVb</td><td>CC388</td><td>ST388</td><td>SL387</td><td>CT9761</td></tr><tr><td>77396</td><td>CLIP 2021/00370</td><td>LISVAL 446</td><td>L. ivanovii subsp. ivanovii</td><td>2020-02-13</td><td>Murcia</td><td>Farm 6</td><td>Animal 48</td><td>cow - tonsil</td><td>56</td><td>2907510</td><td>323421</td><td>37</td><td>n.a.</td><td>n.a.</td><td>L</td><td>CC883</td><td>ST883</td><td>n.a.</td><td>n.a.</td></tr><tr><td>77417</td><td>CLIP 2021/00373</td><td>LISVAL 449</td><td>L. ivanovii subsp. ivanovii</td><td>2020-02-13</td><td>Murcia</td><td>Farm 6</td><td>Animal 48</td><td>cow - udder</td><td>54</td><td>2926679</td><td>348239</td><td>37</td><td>n.a.</td><td>n.a.</td><td>L</td><td>CC883</td><td>ST883</td><td>n.a.</td><td>n.a.</td></tr><tr><td>77420</td><td>CLIP 2021/00376</td><td>LISVAL 452</td><td>L. ivanovii subsp. ivanovii</td><td>2020-02-13</td><td>Murcia</td><td>Farm 6</td><td>Animal 51</td><td>cow - udder</td><td>70</td><td>2926607</td><td>181775</td><td>37</td><td>n.a.</td><td>n.a.</td><td>L</td><td>CC883</td><td>ST883</td><td>n.a.</td><td>n.a.</td></tr><tr><td>77423</td><td>CLIP 2021/00379</td><td>LISVAL 455</td><td>L. ivanovii subsp. ivanovii</td><td>2020-02-13</td><td>Murcia</td><td>Farm 6</td><td>Animal 57</td><td>cow - udder</td><td>57</td><td>2929111</td><td>247159</td><td>37</td><td>n.a.</td><td>n.a.</td><td>L</td><td>CC883</td><td>ST883</td><td>n.a.</td><td>n.a.</td></tr><tr><td>77424</td><td>CLIP 2021/00380</td><td>LISVAL 459</td><td>L. ivanovii subsp. ivanovii</td><td>2020-02-13</td><td>Murcia</td><td>Farm 7</td><td>Animal 49</td><td>cow - udder</td><td>55</td><td>2913115</td><td>352213</td><td>37</td><td>n.a.</td><td>n.a.</td><td>L</td><td>CC883</td><td>ST883</td><td>n.a.</td><td>n.a.</td></tr><tr><td>77429</td><td>CLIP 2021/00385</td><td>LISVAL 605</td><td>L. ivanovii subsp. ivanovii</td><td>2020-06-02</td><td>Valencia</td><td>Farm 15</td><td>Animal 149</td><td>cow - udder</td><td>41</td><td>2900789</td><td>331742</td><td>37</td><td>n.a.</td><td>n.a.</td><td>L</td><td>CC883</td><td>ST883</td><td>n.a.</td><td>n.a.</td></tr><tr><td>77433</td><td>CLIP 2021/00389</td><td>LISVAL 622</td><td>L. ivanovii subsp. ivanovii</td><td>2020-12-04</td><td>Murcia</td><td>Farm 1</td><td>Animal 65</td><td>cow - udder</td><td>62</td><td>2929665</td><td>109996</td><td>37</td><td>n.a.</td><td>n.a.</td><td>L</td><td>CC883</td><td>ST883</td><td>n.a.</td><td>n.a.</td></tr><tr><td>77435</td><td>CLIP 2021/00391</td><td>LISVAL 624</td><td>L. ivanovii subsp. ivanovii</td><td>2020-12-04</td><td>Murcia</td><td>Farm 11</td><td>Animal 132</td><td>cow - udder</td><td>48</td><td>2901466</td><td>164327</td><td>37</td><td>n.a.</td><td>n.a.</td><td>L</td><td>CC883</td><td>ST883</td><td>n.a.</td><td>n.a.</td></tr><tr><td>77436</td><td>CLIP 2021/00392</td><td>LISVAL 625</td><td>L. ivanovii subsp. ivanovii</td><td>2020-12-04</td><td>Murcia</td><td>Farm 12</td><td>Animal 52</td><td>cow - udder</td><td>83</td><td>2954649</td><td>148669</td><td>37</td><td>n.a.</td><td>n.a.</td><td>L</td><td>CC883</td><td>ST883</td><td>n.a.</td><td>n.a.</td></tr><tr><td>77437</td><td>CLIP 2021/00393</td><td>LISVAL 626</td><td>L. ivanovii subsp. ivanovii</td><td>2020-12-04</td><td>Murcia</td><td>Farm 10</td><td>Animal 145</td><td>cow - udder</td><td>36</td><td>2944244</td><td>211155</td><td>37</td><td>n.a.</td><td>n.a.</td><td>L</td><td>CC883</td><td>ST883</td><td>n.a.</td><td>n.a.</td></tr><tr><td>77439</td><td>CLIP 2021/00395</td><td>LISVAL 628</td><td>L. ivanovii subsp. ivanovii</td><td>2020-12-04</td><td>Murcia</td><td>Farm 10</td><td>Animal 142</td><td>cow - udder</td><td>41</td><td>2901256</td><td>224777</td><td>37</td><td>n.a.</td><td>n.a.</td><td>L</td><td>CC883</td><td>ST883</td><td>n.a.</td><td>n.a.</td></tr><tr><td>77441</td><td>CLIP 2021/00397</td><td>LISVAL 630</td><td>L. ivanovii subsp. ivanovii</td><td>2020-12-04</td><td>Murcia</td><td>Farm 5</td><td>Animal 139</td><td>cow - udder</td><td>43</td><td>2944941</td><td>171888</td><td>37</td><td>n.a.</td><td>n.a.</td><td>L</td><td>CC883</td><td>ST883</td><td>n.a.</td><td>n.a.</td></tr><tr><td>77443</td><td>CLIP 2021/00399</td><td>LISVAL 632</td><td>L. ivanovii subsp. ivanovii</td><td>2020-12-04</td><td>Murcia</td><td>Farm 10</td><td>Animal 148</td><td>cow - udder</td><td>104</td><td>2895532</td><td>64194</td><td>37</td><td>n.a.</td><td>n.a.</td><td>L</td><td>CC883</td><td>ST883</td><td>n.a.</td><td>n.a.</td></tr><tr><td>77445</td><td>CLIP 2021/00401</td><td>LISVAL 634</td><td>L. ivanovii subsp. ivanovii</td><td>2020-12-04</td><td>Murcia</td><td>Farm 10</td><td>Animal 147</td><td>cow - tonsil</td><td>111</td><td>2894126</td><td>78390</td><td>37</td><td>n.a.</td><td>n.a.</td><td>L</td><td>CC883</td><td>ST883</td><td>n.a.</td><td>n.a.</td></tr><tr><td>77447</td><td>CLIP 2021/00402</td><td>LISVAL 635</td><td>L. ivanovii subsp. ivanovii</td><td>2020-12-04</td><td>Murcia</td><td>Farm 10</td><td>Animal 141</td><td>cow - feces</td><td>72</td><td>2947364</td><td>210627</td><td>37</td><td>n.a.</td><td>n.a.</td><td>L</td><td>CC883</td><td>ST883</td><td>n.a.</td><td>n.a.</td></tr><tr><td>79911</td><td>CLIP 2021/01132</td><td>LISVAL 767</td><td>L. ivanovii subsp. ivanovii</td><td>2021-05-06</td><td>Valencia</td><td>Farm 15</td><td>Animal 162</td><td>cow - udder</td><td>34</td><td>2901547</td><td>239098</td><td>37</td><td>n.a.</td><td>n.a.</td><td>L</td><td>CC883</td><td>ST883</td><td>n.a.</td><td>n.a.</td></tr></tbody></table>
<table><caption>Table S2. Extended virulence gene patterns in *Listeria* isolates characterized in this study. Boxes with an "x" represent the presence of the genetic trait.</caption><thead><tr><th>BIGSdb id</th><th>isolate</th><th>aliases</th><th>species</th><th>phylogenetic lineage</th><th>genoserogroup</th><th>CC (MLST)</th><th>ST (MLST)</th><th>SL (cgMLST)</th><th>CT (cgMLST)</th><th>gltA</th><th>gltB</th><th>tagB</th><th>aut</th><th>aut_lvb</th><th>inlE</th><th>inlG</th><th>inlH</th><th>inlJ</th><th>inlK</th><th>vip</th><th>oppA</th><th>agrC</th><th>mouR</th><th>mdrM</th><th>comK</th><th>bapL</th><th>ami</th><th>dltA</th><th>fbpA</th><th>lap</th><th>lapB</th></tr></thead><tbody><tr><td>77396</td><td>202100370</td><td>LISVAL 446</td><td>L. ivanovii subsp. ivanovii</td><td>n.a.</td><td>L</td><td>CCB83</td><td>ST883</td><td>n.a.</td><td>n.a.</td><td>X</td><td>X</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>77417</td><td>202100373</td><td>LISVAL 449</td><td>L. ivanovii subsp. ivanovii</td><td>n.a.</td><td>L</td><td>CCB83</td><td>ST883</td><td>n.a.</td><td>n.a.</td><td>X</td><td>X</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>77420</td><td>202100376</td><td>LISVAL 452</td><td>L. ivanovii subsp. ivanovii</td><td>n.a.</td><td>L</td><td>CCB83</td><td>ST883</td><td>n.a.</td><td>n.a.</td><td>X</td><td>X</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>77423</td><td>202100379</td><td>LISVAL 455</td><td>L. ivanovii subsp. ivanovii</td><td>n.a.</td><td>L</td><td>CCB83</td><td>ST883</td><td>n.a.</td><td>n.a.</td><td>X</td><td>X</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>77424</td><td>202100380</td><td>LISVAL 456</td><td>L. ivanovii subsp. ivanovii</td><td>n.a.</td><td>L</td><td>CCB83</td><td>ST883</td><td>n.a.</td><td>n.a.</td><td>X</td><td>X</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>77429</td><td>202100385</td><td>LISVAL 605</td><td>L. ivanovii subsp. ivanovii</td><td>n.a.</td><td>L</td><td>CCB83</td><td>ST883</td><td>n.a.</td><td>n.a.</td><td>X</td><td>X</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>77433</td><td>202100389</td><td>LISVAL 622</td><td>L. ivanovii subsp. ivanovii</td><td>n.a.</td><td>L</td><td>CCB83</td><td>ST883</td><td>n.a.</td><td>n.a.</td><td>X</td><td>X</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>77435</td><td>202100391</td><td>LISVAL 624</td><td>L. ivanovii subsp. ivanovii</td><td>n.a.</td><td>L</td><td>CCB83</td><td>ST883</td><td>n.a.</td><td>n.a.</td><td>X</td><td>X</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>77436</td><td>202100392</td><td>LISVAL 625</td><td>L. ivanovii subsp. ivanovii</td><td>n.a.</td><td>L</td><td>CCB83</td><td>ST883</td><td>n.a.</td><td>n.a.</td><td>X</td><td>X</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>77437</td><td>202100393</td><td>LISVAL 626</td><td>L. ivanovii subsp. ivanovii</td><td>n.a.</td><td>L</td><td>CCB83</td><td>ST883</td><td>n.a.</td><td>n.a.</td><td>X</td><td>X</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>77439</td><td>202100395</td><td>LISVAL 628</td><td>L. ivanovii subsp. ivanovii</td><td>n.a.</td><td>L</td><td>CCB83</td><td>ST883</td><td>n.a.</td><td>n.a.</td><td>X</td><td>X</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>77441</td><td>202100397</td><td>LISVAL 630</td><td>L. ivanovii subsp. ivanovii</td><td>n.a.</td><td>L</td><td>CCB83</td><td>ST883</td><td>n.a.</td><td>n.a.</td><td>X</td><td>X</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>77443</td><td>202100399</td><td>LISVAL 632</td><td>L. ivanovii subsp. ivanovii</td><td>n.a.</td><td>L</td><td>CCB83</td><td>ST883</td><td>n.a.</td><td>n.a.</td><td>X</td><td>X</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>77445</td><td>202100401</td><td>LISVAL 634</td><td>L. ivanovii subsp. ivanovii</td><td>n.a.</td><td>L</td><td>CCB83</td><td>ST883</td><td>n.a.</td><td>n.a.</td><td>X</td><td>X</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>77447</td><td>202100402</td><td>LISVAL 635</td><td>L. ivanovii subsp. ivanovii</td><td>n.a.</td><td>L</td><td>CCB83</td><td>ST883</td><td>n.a.</td><td>n.a.</td><td>X</td><td>X</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>79911</td><td>202101132</td><td>LISVAL 767</td><td>L. ivanovii subsp. ivanovii</td><td>n.a.</td><td>L</td><td>CCB83</td><td>ST883</td><td>n.a.</td><td>n.a.</td><td>X</td><td>X</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>77393</td><td>202100367</td><td>LISVAL 408</td><td>L. monocytogenes</td><td>II</td><td>IIc</td><td>CC9</td><td>ST9</td><td>SL9</td><td>CT9007</td><td>X</td><td>X</td><td>X</td><td>X</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>77394</td><td>202100368</td><td>LISVAL 409</td><td>L. monocytogenes</td><td>II</td><td>IIc</td><td>CC9</td><td>ST9</td><td>SL9</td><td>CT9007</td><td>X</td><td>X</td><td>X</td><td>X</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>77395</td><td>202100369</td><td>LISVAL 410</td><td>L. monocytogenes</td><td>I</td><td>IVb</td><td>CC4</td><td>ST4</td><td>SL4</td><td>CT9158</td><td>X</td><td>X</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>77421</td><td>202100377</td><td>LISVAL 453</td><td>L. monocytogenes</td><td>II</td><td>IIa</td><td>CC37</td><td>ST37</td><td>SL37</td><td>CT1618</td><td>X</td><td>X</td><td>X</td><td>X</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>79286</td><td>202101103</td><td>LISVAL 738</td><td>L. monocytogenes</td><td>I</td><td>IVb</td><td>CC6</td><td>ST6</td><td>SL6</td><td>CT9763</td><td>X</td><td>X</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>79918</td><td>202101139</td><td>LISVAL 774</td><td>L. monocytogenes</td><td>I</td><td>IVb</td><td>CC388</td><td>ST388</td><td>SL387</td><td>CT9761</td><td>X</td><td>X</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>77387</td><td>202100361</td><td>LISVAL 368</td><td>L. innocua</td><td>n.a.</td><td>L</td><td>CC493</td><td>ST493</td><td>n.a.</td><td>n.a.</td><td>X</td><td>X</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>77388</td><td>202100362</td><td>LISVAL 369</td><td>L. innocua</td><td>n.a.</td><td>L</td><td>CC493</td><td>ST493</td><td>n.a.</td><td>n.a.</td><td>X</td><td>X</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>77389</td><td>202100363</td><td>LISVAL 370</td><td>L. innocua</td><td>n.a.</td><td>L</td><td>CC493</td><td>ST493</td><td>n.a.</td><td>n.a.</td><td>X</td><td>X</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>77390</td><td>202100364</td><td>LISVAL 371</td><td>L. innocua</td><td>n.a.</td><td>L</td><td>CC493</td><td>ST493</td><td>n.a.</td><td>n.a.</td><td>X</td><td>X</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>77391</td><td>202100365</td><td>LISVAL 376</td><td>L. innocua</td><td>n.a.</td><td>L</td><td>CC1480</td><td>ST1480</td><td>n.a.</td><td>n.a.</td><td>X</td><td>X</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>77392</td><td>202100366</td><td>LISVAL 377</td><td>L. innocua</td><td>n.a.</td><td>L</td><td>CC599</td><td>ST2753</td><td>n.a.</td><td>n.a.</td><td>X</td><td>X</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>77422</td><td>202100378</td><td>LISVAL 454</td><td>L. innocua</td><td>n.a.</td><td>L</td><td>CC474</td><td>ST474</td><td>n.a.</td><td>n.a.</td><td>X</td><td>X</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>77425</td><td>202100381</td><td>LISVAL 457</td><td>L. innocua</td><td>n.a.</td><td>L</td><td>CC530</td><td>ST530</td><td>n.a.</td><td>n.a.</td><td>X</td><td>X</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>77426</td><td>202100382</td><td>LISVAL 544</td><td>L. innocua</td><td>n.a.</td><td>L</td><td>CC530</td><td>ST530</td><td>n.a.</td><td>n.a.</td><td>X</td><td>X</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>77427</td><td>202100383</td><td>LISVAL 545</td><td>L. innocua</td><td>n.a.</td><td>L</td><td>CC530</td><td>ST530</td><td>n.a.</td><td>n.a.</td><td>X</td><td>X</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>77428</td><td>202100384</td><td>LISVAL 546</td><td>L. innocua</td><td>n.a.</td><td>L</td><td>CC530</td><td>ST530</td><td>n.a.</td><td>n.a.</td><td>X</td><td>X</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>77432</td><td>202100388</td><td>LISVAL 615</td><td>L. innocua</td><td>n.a.</td><td>L</td><td>CC2754</td><td>ST2754</td><td>n.a.</td><td>n.a.</td><td>X</td><td>X</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>79916</td><td>202101137</td><td>LISVAL 772</td><td>L. innocua</td><td>n.a.</td><td>L</td><td>CC1616</td><td>ST1616</td><td>n.a.</td><td>n.a.</td><td>X</td><td>X</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>79917</td><td>202101138</td><td>LISVAL 773</td><td>L. innocua</td><td>n.a.</td><td>L</td><td>CC1480</td><td>ST1480</td><td>n.a.</td><td>n.a.</td><td>X</td><td>X</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>79935</td><td>202101149</td><td>LISVAL 784</td><td>L. innocua</td><td>n.a.</td><td>L</td><td>CC1482</td><td>ST1482</td><td>n.a.</td><td>n.a.</td><td>X</td><td>X</td><td>X</td><td>X</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr></tbody></table>