# Fossil Corals With Various Degrees of Preservation Can Retain Information About Biomineralization-Related Organic Material

Jeana Drake, Maxence Guillermic, Robert A. Eagle, David Jacobs

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Jeana Drake, Maxence Guillermic, Robert A. Eagle, David Jacobs. Fossil Corals With Various Degrees of Preservation Can Retain Information About Biomineralization-Related Organic Material. Frontiers in Earth Science, 2021, 9, 10.3389/feart.2021.643864. hal-03324362

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# Fossil Corals With Various Degrees of Preservation Can Retain Information About Biomineralization-Related Organic Material

Jeana L. Drake<sup>1,2,3*†</sup>, Maxence Guillermic<sup>2,4,5†</sup>, Robert A. Eagle<sup>2,4,5</sup> and David K. Jacobs<sup>2,3*</sup>

<sup>1</sup>Department of Marine Biology, University of Haifa, Haifa, Israel, <sup>2</sup>Department of Earth, Planetary, and Space Sciences, University of California - Los Angeles, Los Angeles, CA, United States, <sup>3</sup>Department of Ecology and Evolutionary Biology, University of California - Los Angeles, Los Angeles, CA, United States, <sup>4</sup>Department of Atmospheric and Oceanic Sciences, Institute of the Environment and Sustainability, University of California - Los Angeles, Los Angeles, CA, United States, <sup>5</sup>Université de Brest Occidentale, Institut Universitaire Européen de la Mer, Plouzané, France

## OPEN ACCESS

**Edited by:**
Claire Rollion-Bard,
UMR7154 Institut de Physique du
Globe de Paris (IPGP), France

**Reviewed by:**
Frederic Marin,
Délégation Centre-Est (CNRS), France
Sergio Rodríguez,
Universidad Complutense de Madrid,
Spain

*Correspondence:
Jeana L. Drake
jeanadrake@g.ucla.edu
David K. Jacobs
djacobs@g.ucla.edu

†These authors have contributed equally to this work and share first authorship

**Specialty section:**
This article was submitted to Biogeoscience, a section of the journal Frontiers in Earth Science

**Received:** 19 December 2020
**Accepted:** 10 June 2021
**Published:** 23 June 2021

**Citation:**
Drake JL, Guillermic M, Eagle RA and Jacobs DK (2021) Fossil Corals With Various Degrees of Preservation Can Retain Information About Biomineralization-Related Organic Material. *Front. Earth Sci.* 9:643864
doi: 10.3389/feart.2021.643864

Scleractinian corals typically form a robust calcium carbonate skeleton beneath their living tissue. This skeleton, through its trace element composition and isotope ratios, may record environmental conditions of water surrounding the coral animal. While bulk unrecrystallized aragonite coral skeletons can be used to reconstruct past ocean conditions, corals that have undergone significant diagenesis have altered geochemical signatures and are typically assumed to retain insufficient meaningful information for bulk or macrostructural analysis. However, partially recrystallized skeletons may retain organic molecular components of the skeletal organic matrix (SOM), which is secreted by the animal and directs aspects of the biomineralization process. Some SOM proteins can be retained in fossil corals and can potentially provide past oceanographic, ecological, and indirect genetic information. Here, we describe a dataset of scleractinian coral skeletons, aged from modern to Cretaceous plus a Carboniferous rugosan, characterized for their crystallography, trace element composition, and amino acid compositions. We show that some specimens that are partially recrystallized to calcite yield potentially useful biochemical information whereas complete recrystalization or silification leads to significant alteration or loss of the SOM fraction. Our analysis is informative to biochemical-paleoceanographers as it suggests that previously discounted partially recrystallized coral skeletons may indeed still be useful at the microstructural level.

**Keywords:** amino acids, trace elements, X-ray diffraction, mineralogy, scleractinians

## INTRODUCTION

Fossil stony corals (phylum Cnidaria, class Anthozoa) are valuable archives for paleoclimatic studies. Elemental and isotopic compositions of their aragonite skeleton can reflect environmental and chemical conditions of seawater in which they grew (e.g., Cohen and McConaughey, 2003), allowing reconstruction of past seawater conditions (e.g., Watanabe et al., 2001; Gothmann et al., 2015). Proteins, polysaccharides, and lipids comprise the skeletal organic matrix (SOM), which is secreted by cells of the calicoblastic tissue layer of the animal (Puverel et al., 2005; Mass et al., 2014; reviewed by Drake et al. (2020a). Of these matrix components, proteins are the best studied; their
preservation in the mineral can lead to inferences of function and
provide indirect genetic information in the absence of preserved
DNA (e.g., Schroeter et al., 2017). Recent analyses have shown
that intact endogenous peptides remain in invertebrate skeletons
for 1000s-100,000s of years (Sakalauskaite et al., 2019; Drake L.
et al., 2020b; Sakalauskaite et al., 2020). Across various taxa, intact
peptides may retain amino acids longer in fossil specimens than if
those biomolecules exist individually as free amino acids (Bada
et al., 1999), allowing for detection of ecological and ocean
chemistry signatures including the occurrence of
photosymbiosis in fossil scleractinian corals (Muscatine et al.,
2005; Tornabene et al., 2017) and past local nitrogen cycling
regimes (Wang et al., 2015).

Nevertheless, the utility of either component of a fossil coral, inorganic or organic, is limited by its preservation (Nothdurft et al., 2007; Rabier et al., 2008). Fossil corals can undergo submarine and/or subaerial post-depositional alterations including dissolution of primary aragonite, precipitation of secondary aragonite cements and/or high-Mg calcite, and recrystallization from aragonite to calcite, with subsequent alteration of the original aragonitic geochemical signature (Brand and Veizer, 1980; Sayani et al., 2011; Griffiths et al., 2013) and potential degradation of proteins with loss of amino acids (Tomiak et al., 2013; Tomiak et al., 2016; Drake et al., 2020b). All primary aragonite corals with minimal macro- and micro-structural inorganic degradation are considered equally, but partially recrystallized corals are often wholly excluded from bulk analysis (e.g., Gothmann et al., 2015), or their recrystallized portions are removed prior to further analysis (e.g., Tornabene et al.). The organic fraction of coral skeleton is typically ~1% or less (Wainwright, 1963), providing insufficient material for microanalysis if it is significantly degraded, particularly if peptide sequences are the target (Peled et al., 2020). While geochemical signatures may be altered in partially recrystallized samples (McGregor and Gagan, 2003; Allison et al., 2007; Sayani et al., 2011), it is not yet clear whether amino acid data from those samples remain useful.

Methods for determining mineralogy, trace element
composition, and amino acid characteristics of coral skeletons
are well-established but are not frequently used together.
Crystallographic determination of calcium carbonate minerals
can be performed non-destructively using x-ray diffraction
(XRD) on ground skeleton powders and can be conducted
quantitatively (Chung, 1974). Geochemical analysis of element/
Ca in such powders by mass spectrometry requires small sample
sizes (<100 µg) and is destructive, yielding relative abundance
information for traditionally measured elements including Li, B,
Mg, Sr, Cd, Ba, U, Na, Mn, Fe that are then standardized to the Ca
signal (Yu et al., 2005; Misra et al., 2014; Guillermic et al., 2020).
Amino acid composition and racemization analysis is a
destructive method and requires a larger amount of skeleton
material (typically 1-10 s mg), yielding data about a subset of the
20 common naturally occurring amino acids (Kaufman and
Manley, 1998). Amino acid racemization is the reversible
process wherein one enantiomer converts to the other over
time and is affected by environmental conditions such as
temperature (Neuberger, 1948). As most organisms on Earth

produce and use the L enantiomer of amino acids (Bai et al., 2009; reviewed by Genchi (2017), the initial conversion is generally considered to be from the L to D form; D/L ratios of certain amino acids can thus be applied as a relative or specific dating tool for fossils from the late Pleistocene to the present (Bada, 1985; Kaufman, 2003; Demarchi, 2020). Use of all three tools for the same specimens can therefore clarify the preservation of not just the abiotic component of fossil corals, but the biomolecular component as well, while using a relatively small amount of material.

Here we describe our analysis of coral skeletons ranging in age
from modern to putatively Cretaceous, plus a Carboniferous
rugosan coral. We analyzed the mineralogy, geochemistry, and
amino acid characteristics for these corals and show that even
partially recrystallized fossil coral skeletons likely retain
important biochemical information. This work provides a
framework for biochemists and paleoceanographers to
consider expanding the pool of potential specimens for analysis.

METHODS

Samples

Fossils specimens were loaned from the Natural History Museum of Los Angeles County (NHMLAC) Invertebrate Paleontology Department, American Museum of Natural History (AMNH) Paleontology Division, Yale Peabody Museum (Peabody) Invertebrate Paleontology Division, and Harvard Museum of Comparative Zoology (MCZ) Invertebrate Paleontology Collection (Table 1) (Zoology, 2020). One modern coral was loaned from the NHMLAC Marine Biodiversity Center while another was provided from a private research collection. Provenance of fossil corals is reliant on collection location information on file at the loaning museums and in some cases age assignments are questionable based on the scientific literature (Table 1). For instance, we can with some confidence assign the Pleistocene *Montastraea cavernosa* and *Orbicella annularis* corals to stage 5e of the Key Largo formation (Hendy et al., 2020); however, we cannot absolutely rule out that it was actually derived from stage 4 (Multer et al., 2002; Muhs et al., 2011). Elemental, mineralogical, amino acid, and proteomic data for two fossil *O. annularis* specimens and one *M. cavernosa* have been previously reported (Drake et al., 2020b).

Cleaning

All specimens were slabbed on a water saw to use <~10% of the
borrowed coral head. Fragments were cleaned of organic material
in equal parts 3% bleach and 50% hydrogen peroxide, milled or
ground, and cleaned three further times in bleach/hydrogen
peroxide for 30 min (milled powder) or overnight (ground
powder) each cleaning followed by copious rinsing using
modified methods of (Stoll et al., 2001). Several checks of the
final product suggest that only endogenous intracrystalline SOM
remained (Drake et al., 2020b). Samples for XRD were milled
using a #2 carbide bur while samples for amino acid analysis were
ground to <125 µm on an agate mortar and pestle and sieved.
Cleaned coral powder analyzed for amino acid parameters was
<table><caption>**TABLE 1** | Modern and fossil coral specimen descriptions including data provided by the lender, verification of stated ages based on stratigraphic information confirmed in the literature, and mineralogy (A = aragonite, C = calcite) averaged between samples milled within Vs. between corallites or at different layers within the sample. Specimens loaned by the AMNH, NHMLAC, Peabody and MCZ have been returned to the lenders; specimens from the private research collection remain in the possession of the authors at this time.</caption><thead><tr><th>Order</th><th>Accepted Species Name</th><th>Species Name in Collection + # Code Here</th><th>Source Location</th><th>Lender Stated Aged</th><th>Age Valid Based on Stratigraphic Information?</th><th>Age Based on Scientific Literature</th><th>Age Validation or Invalidation References</th><th>Museum</th><th>Cabinet</th><th>Drawer</th><th>Collection Date</th><th>Collector or Donation Collection</th><th>Catalog or Specimen #</th><th>Acquisition #</th><th>Average %A/C</th></tr></thead><tbody><tr><td>Scleractinia</td><td>Pocillopora damicornis</td><td>Pocillopora damicornis (modern)</td><td>Oluwalu, HI (Olowalu, Maui, HI), 3 feet</td><td>modern</td><td>yes</td><td>Modern</td><td>—</td><td>LANHM</td><td>Stony corals full</td><td>4</td><td>—</td><td>Freddie Curtis</td><td>11140</td><td>A8693.66</td><td>100/0</td></tr><tr><td>Scleractinia</td><td>Orbicella annularis</td><td>Orbicella annularis (modern)</td><td>Coral Gardens, south of southern tip of Ambergris Island, Belize</td><td>modern</td><td>Yes</td><td>modern</td><td>—</td><td>Lisa Greer</td><td>—</td><td>—</td><td>—</td><td>L. Greer</td><td>—</td><td>—</td><td>100/0</td></tr><tr><td>Scleractinia</td><td>Porites panamensis</td><td>Porites californica</td><td>"Puritan", F-30, Marques Bay, Carmen Island, Gulf of California</td><td>Pliocene</td><td>yes</td><td>Upper Pliocene</td><td>Durham 1950 GSA Memoir 43</td><td>AMNH</td><td>244</td><td>1</td><td>—</td><td>—</td><td>AMNH-FI-113436 (old #F-30)</td><td>—</td><td>100/0</td></tr><tr><td>Scleractinia</td><td>Orbicella annularis</td><td>Montastraea annularis 3</td><td>East side of Key Largo, Dade County, FL</td><td>Pleistocene</td><td>yes</td><td>125138 ka, Pleistocene</td><td>Fruitjer et al. (2000) GSA Bulletin; Multer et al. (2002) Facies</td><td>LANHM</td><td>Pleistocene Florida</td><td>—</td><td>12/15/75</td><td>E. Wilson</td><td>LACMIP 5111.3</td><td>5111</td><td>100/0</td></tr><tr><td>Scleractinia</td><td>Orbicella limbata</td><td>Orbicella limbata</td><td>San Domingo</td><td>—</td><td>—</td><td>no information to support age</td><td>—</td><td>MCZ</td><td>—</td><td>—</td><td>—</td><td>—</td><td>MCZ:IP: 196571</td><td>—</td><td>100/0</td></tr><tr><td>Scleractinia</td><td>Porites nodifera</td><td>Porites clavaria</td><td>1/2 mile west of Port Limon, Costa Rica</td><td>—</td><td>—</td><td>Probaly Pliocene in Rio Banano formation</td><td>Taylor 1975 LSU dissertation</td><td>MCZ</td><td>—</td><td>—</td><td>1895</td><td>Robert T. Hill</td><td>MCZ:IP: 196568</td><td>—</td><td>100/0</td></tr><tr><td>Scleractinia</td><td>Stylophora affinis</td><td>Stylophora affinis</td><td>San Domingo</td><td>Miocene</td><td>?</td><td>no information to support age</td><td>—</td><td>MCZ</td><td>—</td><td>—</td><td>—</td><td>William More Gabb Kuhn</td><td>MCZ:IP: 196570 AMNH-FI-113437 (old #13)</td><td>—</td><td>100/0</td></tr><tr><td>Scleractinia</td><td>Porites maigensis</td><td>Porites maigensis</td><td>Burdigal; Maigen bei Eggenburg, Niederosterreich (Lower Austria)</td><td>Miocene</td><td>Yes</td><td>Miocene</td><td>Nebelsick 1989 Facies</td><td>AMNH</td><td>243</td><td>15</td><td>—</td><td>—</td><td>—</td><td>—</td><td>96.5/3.5</td></tr><tr><td>Scleractinia</td><td>Orbicella annularis</td><td>Montastraea annularis 4</td><td>East side of Key Largo, Dade County, FL</td><td>Pleistocene</td><td>yes</td><td>125-138 ka, Pleistocene</td><td>Fruitjer et al. 2000; Multer et al. (2002)</td><td>LANHM</td><td>Pleistocene Florida</td><td>—</td><td>12/15/75</td><td>E. Wilson</td><td>LACMIP 5111.4</td><td>5111</td><td>93.5/6.5</td></tr><tr><td>Scleractinia</td><td>Montastraea cavernosa</td><td>Orbicella cavernosa</td><td>San Domingo</td><td>—</td><td>—</td><td>no information to support age</td><td>—</td><td>MCZ</td><td>—</td><td>—</td><td>—</td><td>William More Gabb</td><td>MCZ:IP: 196576</td><td>—</td><td>86.5/13.5</td></tr><tr><td>Scleractinia</td><td>Porites sp.</td><td>Porites sp.</td><td>Sab. Inf.; Anvers, France</td><td>Upper Eocene</td><td>no</td><td>Oligocene to Pliocene</td><td>Lankester 1865 Geological Magazine</td><td>AMNH</td><td>243</td><td>15</td><td>—</td><td>—</td><td>AMNH-FI-2980-1</td><td>—</td><td>85/15</td></tr><tr><td>Scleractinia</td><td>Orbicella annularis</td><td>Montastraea annularis 6</td><td>East side of Key Largo, Dade County, FL</td><td>Pleistocene</td><td>yes</td><td>125-138 ka, Pleistocene</td><td>Fruitjer et al. 2000 GSA Bulletin; Multer et al. (2002) Facies</td><td>LANHM</td><td>Pleistocene Florida</td><td>—</td><td>12/15/75</td><td>E. Wilson</td><td>LACMIP 5111.6</td><td>5111</td><td>85/15</td></tr><tr><td>Scleractinia</td><td>Montastraea cavernosa</td><td>Montastraea cavernosa 1</td><td>East side of Key Largo, Dade County, FL</td><td>Pleistocene</td><td>yes</td><td>125-138 ka, Pleistocene</td><td>Fruitjer et al. 2000 GSA Bulletin; Multer et al. (2002) Facies</td><td>LANHM</td><td>Pleistocene Florida</td><td>—</td><td>12/15/75</td><td>E. Wilson</td><td>LACMIP 5111.7</td><td>5111</td><td>77.5/22.5</td></tr></tbody></table>

(Continued on following page)
<table><caption>**TABLE 1** | (Continued) Modern and fossil coral specimen descriptions including data provided by the lender, verification of stated ages based on stratigraphic information confirmed in the literature, and mineralogy (A = aragonite, C = calcite) averaged between samples milled within Vs. between corallites or at different layers within the sample. Specimens loaned by the AMNH, NHMLAC, Peabody and MCZ have been returned to the lenders; specimens from the private research collection remain in the possession of the authors at this time.</caption><thead><tr><th>Order</th><th>Accepted Species Name</th><th>Species Name in Collection + # Code Here</th><th>Source Location</th><th>Lender Stated Aged</th><th>Age Valid Based on Stratigraphic Information?</th><th>Age Based on Scientific Literature</th><th>Age Validation or Invalidation References</th><th>Museum</th><th>Cabinet</th><th>Drawer</th><th>Collection Date</th><th>Collector or Donation Collection</th><th>Catalog or Specimen #</th><th>Acquisition #</th><th>Average %A/C</th></tr></thead><tbody><tr><td>Scleractinia</td><td>No accepted name</td><td>Favia distans</td><td>San Domingo</td><td>Miocene</td><td>?</td><td>no information to support age may be Pliocene</td><td>—</td><td>MCZ</td><td>—</td><td>—</td><td>—</td><td>William More Gabb F.C. Nicholas</td><td>MCZ:IP: 196574 AMNH-FI-11474-1</td><td>—</td><td>53/47</td></tr><tr><td>Scleractinia</td><td>Stylophora conferta</td><td>Stylophora conferta</td><td>Bowden Beds, Bowden, Jamaica, WI</td><td>Miocene</td><td>?</td><td></td><td>Budd 2000 Coral Reefs; Woodring 1928 Carnegie Institute Publication</td><td>AMNH</td><td>242</td><td>5</td><td>—</td><td></td><td></td><td></td><td>35/65</td></tr><tr><td>Scleractinia</td><td>Orbicella annularis</td><td>Montastraea annularis 2</td><td>East side of Key Largo, Dade County, FL</td><td>Pleistocene</td><td>yes</td><td>125-138 ka, Pleistocene</td><td>Fruitjer et al. (2000) GSA Bulletin; Multer et al. (2002) Facies</td><td>LANHM</td><td>Pleistocene Florida</td><td>—</td><td>12/15/75</td><td>E. Wilson</td><td>LACMIP 5111.2</td><td>—</td><td>0/100</td></tr><tr><td>Scleractinia</td><td>Favia sp</td><td>Favia sp. 1</td><td>Nuevitas, Cuba</td><td>Late Cretaceous</td><td>probably</td><td>Upper Cretaceous</td><td>Albear 1947 AAPG Bulletin</td><td>Peabody</td><td>—</td><td>—</td><td>—</td><td>Braun-Schuchert Collection</td><td>539715</td><td>4207</td><td>0/100</td></tr><tr><td>Scleractinia</td><td>Favia sp</td><td>Favia sp. 2</td><td>St. Trinita di Mantechi Magiore (actually Trinita di Montecchio Maggiore)</td><td>Pliocene</td><td>?</td><td>could also be Oligocene</td><td>Budd and Bossellini 2016 J Systematic Paleontology</td><td>Peabody</td><td>—</td><td>—</td><td>—</td><td>Braun-Schuchert Collection</td><td>539716</td><td>4207</td><td>0/100</td></tr><tr><td>Rugosa</td><td>Zaphrenthis dalli</td><td>Zaphrentis dalli</td><td>Burlington Limestone, Burlington, IA</td><td>—</td><td>—</td><td>Mississippian, Carboniferous</td><td>Lauden 1937 AAPG Bulletin</td><td>AMNH</td><td>242</td><td>6</td><td>—</td><td>James Hall</td><td>AMNH-FI-6758-1</td><td>—</td><td>0/5 (95 % quartz)</td></tr></tbody></table>
always handled in a glove bag or laminar flow hood to avoid biological contamination.

## X-Ray Diffraction

Sub-samples, ~5–20 mg, of cleaned coral powder from inside vs. between corallites, newer vs. older material, or on two randomly chosen spots per slab were analyzed for mineral content by XRD on a Panalytical X'Pert Pro X-ray Powder Diffractometer. Powders were run on a zero diffraction background plate. Spectra were analyzed in X'Pert Hi-Score software and relative amounts of aragonite and calcite were determined by the Reference Intensity Ratio method (Chung, 1974) using 01-072-1652 calcite and 00-041-1475 aragonite references for all samples. An internal reference standard was not applied as powders were also intended for further mass spectrometry analysis. Sample preparation by milling could induce recrystallization from aragonite to calcite (Gill et al., 1995; Foster et al., 2008; Waite and Swart, 2015); we therefore also performed XRD analyses on ground and cleaned powders from a subset of samples with mixed mineralogy, yielding similar results between the two preparation methods (Supplementary Figure S1). This suggests that preparation in our study did not impact our mineralogy data.

## Elemental Analysis

Sub-samples used for XRD analysis were then analyzed for trace elemental composition. Powders were cleaned following (Barker et al., 2003) including a clay removal step, an oxidative step and a final dilute acid leach in 0.001 N HCl. Samples were then dissolved in 1N HCl and analyzed for Ca concentration and trace elements (e.g., X/Ca ratios). Ca concentrations were analyzed using an ICP-AES ® Ultima 2 HORIBA and trace elements using a Thermo Scientific ® Element XR (HR-ICP-MS) at Ifremer (Plouzané, France). Samples were diluted to fixed calcium concentrations (30 ppm Ca) using 0.1 M HNO₃ and 0.3 M HF matching multi-element standards Ca concentration to avoid any matrix effects (Misra et al., 2014; Guillermic et al., 2020). External reproducibility and analytical uncertainty were determined on the consistency standard Cam-Wuellestorfli (courtesy of the University of Cambridge) (Misra et al., 2014).

## Amino Acid Content and Racemization

Proteins in ground skeleton powder were hydrolyzed to amino acids following methods of Penkman et al. (2008) with modifications. Briefly, ~20–30 mg cleaned skeleton powder was dissolved in 2 M HCl for 1 h at room temperature (10 ml acid/mg powder). The sample was then dried under a stream of N₂ (Ultra High Purity) and stored at -30°C until hydrolysis. Residues were hydrolyzed to amino acids in 7 M HCl (20 ml acid/mg starting material) at 110°C for 24 h, allowed to cool, then dried first under an N₂ stream and then in a Speed Vac at room temperature. Samples terminated after the leaching phase were analyzed directly for free amino acids (FAA). Samples carried through to full hydrolysis in concentrated acid were analyzed for total hydrolyzable amino acids (THAA).

All samples were prepared in duplicate and analyzed at the Northern Arizona University Amino Acid Geochronology

Laboratory using standard methods with modifications optimized for microfossils (Kaufman and Manley, 1998; Kaufman, 2000; Kaufman, 2003). Rehydrated samples were spiked with L-homo-arginine as an internal standard and then injected into an HPLC fitted with a reverse-phase C18-packed column. “Blank” samples were included. D and L enantiomers of Asx (aspartic acid plus asparagine), Glx (glutamic acid plus glutamine), Ser (serine), Ala (alanine), Val (valine), Phe (phenylalanine), Leu (leucine), and Ile (isoleucine) were quantified for each sample and we calculated the average relative proportion of each total amino acid (D plus L) within that pool.

## Scanning Electron Microscopy

Thin sections 30 µm thick were prepared with ¼-µm diamond polishing. These were gold-coated and then analyzed on a Tescan Vega-3 SMU variable-pressure scanning electron microscope.

## Statistics

Amino acid data were log-transformed and analyzed by principal component analysis in Past4.01 with 1,000 bootstrap replicates (Hammer et al., 2001). Wilcoxon rank sum tests and Kruskal-Wallis H tests of amino acid data were performed in R Studio (R Studio Team, 2019). Statistical comparisons for the trace elements between mineralogies were made using unpaired t-tests (GraphPad Prism version 7.03, GraphPad Software, San Diego, CA United States).

## RESULTS

### Elemental Ratios

All samples except the rugosan, *Zaphrenthis dalli*, were calcium carbonate although some had recrystallized partially or fully to calcite (Table 1; Supplementary Figure S2). *Z. dalli* was almost completely silicified and was therefore not analyzed for elemental composition but was processed for amino acid analysis to represent an extreme outlier in terms of the effects of diagenesis on amino acid properties of hexacoral exoskeletons. We compared the B/Ca, Sr/Ca, and Mg/Ca values of all corals to the range known from modern corals (Figure 1, Supplementary Material, Supplementary Table S1). Samples that are >99% aragonite present B/Ca, Mg/Ca, Sr/Ca, and Ba/Ca in the range of modern corals (95% confidence interval), supporting that they remain primary aragonite. This was also observed in the Li/Ca and Mn/Ca compositions except for *M. annularis* 4 and *O. limbata* presenting higher Li/Ca and Mn/Ca. Samples partially recrystallized to calcite (between 7 and 50%) also fell in the range of modern corals for Mg/Ca, Sr/Ca, Li/Ca, and/ or Ba/Ca (95% confidence interval); this is also true for B/Ca except for *Porites* sp., *S. conferta*, and *M. cavernosa* 1. Samples completely recrystallized to calcite (>99%) present significantly lower B/Ca, Sr/Ca and higher Mg/Ca in comparison to primary aragonitic samples and modern corals.
<figure><img src="image_3.png" /><figcaption>**FIGURE 1** | Boron (A), strontium (B), magnesium (C), lithium (D), barium (E), and manganese (F) relative abundance in fossil coral skeleton by individual specimen (A–C). Solid grey bars indicate the range of element/Ca ratios from modern corals (see Supplementary Material). Hatched grey bars represent the range of boron, strontium and magnesium in published fossil corals for aragonite (>99%) and calcite (>99%) (de Villiers et al., 1994; Quinn and Sampson, 2002; Swart et al., 2002; Allison et al., 2007; Hendy et al., 2007; Nothdurft and Webb, 2009; Griffiths et al., 2013; Hathorne et al., 2013; Gothmann et al., 2015). In line with previous studies, fully recrystallized skeletons from aragonite to calcite show disruption of their elemental compositions. Except for *Porites* sp. and *S. conferta*, mixed mineralogies (up to 50% aragonite/calcite), present boron, strontium and magnesium within the range of aragonitic modern samples, samples recrystallized to calcite (>99%) present lower B/Ca, lower Sr/Ca and higher Mg/Ca relative to modern corals (unpaired *t*-test, *p* < 0.001).</figcaption></figure>

## Amino Acids

Next we took a targeted approach to the amino acid data from all fossils, including *Z. dalli*, and some modern coral skeletons used in previous coral skeletal proteomics analyses (Drake et al., 2020b) using principal component analysis (PCA). We examined the amino acid relative composition (% THAA and FAA Asx, Glx), fraction of THAA that is FAA, total pmol amino acids per mass of skeleton, racemization (D/L THAA and D/L FAA Asx, Glx, and Ser) and THAA and FAA L-Ser/L-Asx across the samples. As expected, D/L THAA and FAA Asx were generally reflective of coral stratigraphic ages, with higher D/L ratios for most of the older corals. We observed a pattern in which 1) some specimens partially recrystallized to calcite clustered in the general direction of unmodified modern and fossil specimens from which SOM proteins have successfully been sequenced, and 2) several fully primary aragonite specimens that were not a part of this broad cluster despite exhibiting trace element composition within the range of modern aragonitic specimens (Figure 2; Supplementary Table S2). Further, we observed that all corals fully recrystallized to calcite except one, or silicified, showed completely unique amino acid characteristic patterns. Combined,

PCs 1 and 2 explain 83% of the variance within the PCA. Going forward, we now classify each fossil specimen as “Good candidate-aragonite,” “Poor candidate-aragonite,” “Good candidate-other,” “Poor candidate-other,” or “Silicified,” and we separately group the modern specimens for which a skeletal proteome has been sequenced.

To examine the driving patterns of these broad clusters, we specifically looked for differences between the corals according to mineralogy for % THAA Asx, THAA L-Ser/L-Asx, and total pmol peptide amino acids per mass of skeleton. For these three parameters, coral skeletal proteins are known to be biased toward the acidic residues although relative abundances of all amino acids vary across taxa (Young, 1971; Mass et al., 2012; Tomiak et al., 2016), these values may reflect contamination by modern amino acids (Kaufman, 2006), and a lower proportion of FAA in the THAA pool may reflect the maintenance of primary peptide sequence information, respectively. All fossil specimens considered to be “good candidates”, regardless of mineralogy, displayed similar % THAA Asx to modern specimens from which skeletal proteomes have been successfully sequenced, whereas “poor candidates” exhibited significantly lower %
<figure><img src="image_4.png" /></figure>

**FIGURE 2** | PCA of log transformed amino acid relative composition and racemization data for all fossil corals plus representative modern corals. Specimens that are >99% aragonite are noted by diamonds, specimens that are a blend of aragonite and calcite are noted by circles, and specimens fully recrystallized to calcite are noted by squares, following the same symbol coding as in **Figure 1**; the silicified specimen is noted by a plus sign.

THAA Asx ($p < 0.05$; **Figure 3A; Supplementary Table S3**). Similarly, “good candidates” and modern specimens did not differ in their THAA L-Ser/L-Asx whereas “poor candidates” exhibited significantly higher values than aragonitic or mixed mineralogy “good candidates” ($p < 0.05$; **Figure 3A; Supplementary Table S3**). We also note a clear trend in pmol peptide-associated amino acids per mg skeleton to be higher in the “good candidates”, although our statistical power for this measure was not high enough to detect significant differences.

## DISCUSSION

Past paleoceanographic reconstruction studies using fossil corals have safely limited their specimen choices to those that contain <1% non-aragonite material (e.g., Allison et al., 2007; Gothmann et al., 2015). This is warranted as, for example, inclusion of 1% high-Mg calcite leads to a 1°C temperature reconstruction from geochemical data (Allison et al., 2007). However, based on data presented here, we propose that selecting corals with <50% recrystallization to calcite that have trace element ratios and amino acid composition in the range of modern coral skeletons will allow the expansion of specimens for biomineral organic matter analysis.

## Mineral Preservation, Diagenesis and Elemental Composition

XRD data show clear evidence of diagenetic effects, with some samples partially or completely recrystallized to calcite, or in the case of *Z. dalli*, silicified. The elemental compositions of the 100% aragonite and 100% calcite samples are consistent with diagenetic effects and associated mineralogies; however, samples recrystallized up to 50% calcite present B/Ca, Sr/Ca and Mg/Ca signatures within the range of modern aragonitic corals and the 100% aragonite fossil samples (**Figure 3**). There appears to be a transition to more block-like crystal morphologies with increasing amounts of calcite (**Supplementary Figure S3**). Samples completely recrystallized to calcite present elemental composition outside the range of modern corals but in line with inorganic precipitation experiments and thermodynamic redistribution of the trace elements (McGregor and Gagan, 2003; Allison et al., 2007; Griffiths et al., 2013; Gothmann et al., 2015 among others).

The discrimination of an element substituting for Ca²⁺ in the crystal lattice is predicted by its size and the thermodynamic equilibrium of the reactions (Brand and Veizer, 1980; Menadakis et al., 2009). If this element is larger than Ca²⁺, it is predicted to be discriminated against in tighter rhombohedral calcite compared to orthorhombic aragonite. This is observed from inorganic
<figure><img src="image_5.png" /></figure>

**FIGURE 3** | Amino acids % THAA Asx (A), THAA L-Ser/L-Asx (B), pmol peptide-AA/mg skeleton (C), boron (D), strontium (E), magnesium (F), lithium (G), barium (H), and manganese (I) abundance of fossil coral skeleton by % aragonite (D–F). In (D–F), Solid grey bars indicate the range of element/Ca ratios from modern corals (see Supplementary Material), (E) dotted line is the linear regression between Sr/Ca and % aragonite from McGregor and Gagan, (2003). With few exceptions, those results suggest that some recrystallized skeletons can be suitable for peptide analyses and that trace element data are useful indicators of potential good and poor candidates for organic matter analyses. In this study, most "poor candidates" showed disruption of elemental ratios compared to modern aragonitic samples (e.g., lower B/Ca, lower Sr/Ca and higher Mg/Ca relative to modern corals).

precipitation experiments for Sr²⁺, Ba²⁺, Li⁺ (e.g., higher concentration in aragonite than calcite), and Mg²⁺ and Mn²⁺ (e.g., lower concentration in aragonite than calcite) (Shen et al., 1991; Gabitov et al., 2011; DeCarlo et al., 2015; Mavromatis et al., 2018; Gabitov et al., 2019). For boron, inorganic precipitations also present higher concentrations in aragonite than calcite (Mavromatis et al., 2015). Across studies examining diagenetic effects on fossil corals on geochemical patterns, subaerial diagenesis (e.g., dissolution and precipitation of secondary calcite from meteoric water) has been shown to cause a decrease in Sr/Ca and increase in Mg/Ca. Submarine diagenesis, including precipitation of secondary aragonite presents higher Sr/Ca (Sayani et al., 2011) but lower Mg/Ca, and/or precipitation of high-Mg calcite presents a decrease in Sr/Ca (Allison et al., 2007) and an increase in Mg/Ca, in line with inorganic precipitation.

Trace element composition of all samples fully recrystallized to calcite are in line with dominant subaerial diagenesis in an open system as we observe elemental ratios consistent with inorganic calcite precipitation (e.g., low Sr/Ca, Ba/Ca, B/Ca, Li/Ca, and high Mg/Ca). This suggests that all should be

excluded from further analysis, but we do note that one fully recrystallized sample (*M. annularis* 2) is classified as a potential good candidate based on amino acid analysis. In contrast, partially recrystallized samples’ trace element and amino acid data suggest that some useful organic matter may remain as described below. Although we do not have the original geochemical signal of the fossil samples, some partially recrystallized to calcite (up to 50% for *Favia distans*) seem to retain the aragonitic signature based on the range of modern corals (Figures 1, 3). This can potentially be attributed to simultaneous dissolution/precipitation (e.g., closed system) from subaerial diagenesis which is thought to retain the primary signature (Sayani et al., 2011). Li/Ca and Mn/Ca ratios are more variable than the other elemental ratios explored in this study. Specifically, the >99% aragonite corals *O. limbata* and *M. annularis* 4 present Li/Ca and Mn/Ca ratios, potentially reflecting the presence of secondary aragonite precipitated from a reducing diagenetic fluid (Gothmann et al., 2015). In all cases, the disruptions of the Li/Ca and Mn/Ca ratios do not appear correlated with the preservation of the SOM, since *O. limbata* is considered a poor candidate and *M. annularis* 4 a
good candidate for amino acid analyses. Further investigations of the isotopic composition (for example δ¹⁸O) would be useful to deconvolve the diagenesis patterns and their link with amino acid preservation.

## Amino Acid Preservation and Degradation

Inorganic and organic alteration of biominerals, including both protein fragmentation and amino acid loss and increases in crystal size, respectively, occur simultaneously and continuously (Hare, 1980; Tuross et al., 1989). Retention of the organic phase implies that the inorganic component has remained stable, with organic molecules only available for microbial degradation and loss upon alteration of the inorganic phase (Collins et al., 2002). However, organic molecules can become altered within intact mineral, with higher rates observed with increased temperatures, increased absolute and varying amounts of water, marine vs. freshwater conditions, and presence of oxygen and/or sugars (Hare, 1962; Hare, 1974; 1980; Painter, 2003; McCobb et al., 2004), all of which are associated with aerial localization or microbial degradation of the specimen (McGregor and Gagan, 2003; Sayani et al., 2011). Specifically in invertebrates, alteration of the inorganic phase may also allow the intrusion of exogenous amino acids, particularly the abundant metabolic by-product Ser (review by (Mitterer, 1993). Four of seven fossil coral specimens studied here were partially (up to 50%) recrystallized to calcite, yet retained more Asx (THAA fraction) and peptide-associated amino acids and exhibited lower L-Ser/L-Asx (THAA fraction) than other calcite-recrystallized specimens and also fell within the 95% confidence interval of modern corals' B/Ca, Sr/Ca and Mg/Ca (Figures 2, 3), suggesting that the recrystallization in these "good candidate" <99% aragonite specimens likely occurred quickly in a sub-aerial locale, minimizing both degradation and loss of proteinaceous matter and modification of trace element content. B/Ca, Sr/Ca and Mg/Ca analyses may therefore be useful in revealing a larger pool of potential fossil coral candidates including samples partially recrystallized to calcite for organic matter analyses. However, geochemical metrics alone do have limitations, with two of five samples that have fully aragonitic mineralogical and elemental signatures, plus two partially recrystallized samples with aragonitic trace element composition, displaying likely highly degraded proteins. This indicates that the processes that lead to geochemical alteration cannot be assumed to equally alter organic matter. Further, major mineral alterations such as silicification may lead to changes to amino acid content similar to conversion to >50% calcite, as exhibited by high L-Ser/L-Asx (THAA fraction) and low pmol amino acids per mass of skeleton of the 95% silicified rugosan coral, *Z. dalli*, collected from the Burlington formation which is lower to middle Mississippean in age (240–250 mya). There are numerous records of the genus *Zaphrenthis* from the Burlington (Bassler, 1950) Examinations of hypercalcifying non-scleractinian hexacorals such as rugose and tabulate corals, particularly of varying degrees of preservation, will clarify the degree of organic matter preservation in these important fossils as well.

Coral skeletons are not homogenous and the solubility of the different inorganic phases may impact mineral organic matter

alteration. Rapid accretion deposits (RADs) display disorganized crystal orientations, presence of amorphous calcium carbonate, higher Sr/Ca and Mg/Ca, higher skeletal organic matter content, and larger defect sizes compared with thickening deposits (TDs) (e.g., Cuif and Dauphin, 1998; Meibom et al., 2006; Meibom et al., 2008; Brahmi et al., 2012; Rollion-Bard and Blamart, 2015; Mass et al., 2017), likely making them more soluble. Incorporation of specific highly acidic proteins such as the coral acid rich proteins 1 and coral acid rich protein 4, which are abundant in Asp, is also apparent in RADs (Mass et al., 2014). Therefore, coral skeletons exposed to environmental conditions that encourage inorganic phase alterations would likely display those changes preferentially in the RADs yielding localized element/Ca composition alteration, protein fragments and amino acids loss, loss of specific Asp-containing proteins in particular, and Ser intrusion. However, the altered trace element signal may not be detectable by the analysis of bulk skeleton conducted here, as the modern species-specific variation in trace elements between RADs and TDs falls within the modern intra-specific range in bulk values (Cuif and Dauphin, 1998; **Figure 1**). Hence, geochemical analyses may suggest that the bulk skeleton is unaltered or minimally altered (Figures 1, Figures 3D–F), whereas microstructural changes (not tested here) and consequent degradation and loss of organic matter may be observed (Figures 3A–C). Future studies that focus on the organic content of these microstructures will further reveal the effects of localized biomineral degradation on SOM preservation.

## CONCLUSION

As the biological components of fossil bio-carbonates are increasingly used to reconstruct past ocean chemical conditions and ecological processes (Muscatine et al., 2005; Wang et al., 2015; Tornabene et al., 2017), it is important that biomolecules be in an optimal state of preservation. In the present study, combined XRD and elemental ratios were used to assess the preservation and bulk diagenesis of fossil coral samples. Percent THAA Asx, THAA L-Ser/L-Asx and the peptide AA/mg skeleton were then used to assess the potential “good candidates” for organic matter analyses in fossil corals presenting various degrees of recrystallisation. Our results support the exclusion of fully calcite-recrystallized and silicified samples in studies of fossil organic matter. However, some samples partially recrystallized to calcite (up to 50%) were characterized as potential “good candidates” for organic matter analyses.

The present work shows that geochemical analysis is a good first step toward detecting organic matter diagenesis of fossil biominerals. However, this alone does not capture the entire story, not ruling out fully primary aragonite specimens that appear to have experienced localized organic matter degradation and loss while rejecting partially recrystallized samples that appeared to retain useful biological information. We are not the first to advocate for a multidisciplinary approach when choosing biominerals that have gone through some degree of degradation and/or preservation (Tuross et al., 1989). We
suggest that standard amino acid analysis, including the calculation of peptide-associated amino acid content from both THAA and FAA analyses, be added to geochemists' and paleoceanographers' methodological repertoire to carefully expand the pool of bio-carbonates used to study biologically-associated oceanographic processes.

## DATA AVAILABILITY STATEMENT

The original contributions presented in the study are included in the article/Supplementary Material, further inquiries can be directed to the corresponding authors.

## AUTHOR CONTRIBUTIONS

JD and MG designed the study, and collected and analyzed data. Geochemical method development and analyses were supported as part of projects led by RE. DJ provided guidance on interpreting stated fossil coral ages. All authors contributed to writing the manuscript.

## FUNDING

JD was supported by an NSF Postdoctoral Research Fellowship in Biology award #1611943 and the Zuckerman STEM postdoctoral leadership program. MG and the trace element analysis was supported by IAGC (grant no. IAGC student grant 2017), a LabexMer (grant no. ANR-10-LABX-19-01 to Robert Eagle and Aradhna Tripati (UCLA and Institut Universitaire Européen de la

Mer) and a DOE BES grant (grant no. DE-FG02-13ER16402) to Aradhna Tripati (UCLA). RE also acknowledges support from National Science Foundation Grant #1437166 and the Pritzker Endowment to UCLA.

## ACKNOWLEDGMENTS

We thank Austin Hendy, Lindsay Walker, and Kathy Omura (Natural History Museum of Los Angeles County), Melanie Hopkins and Bushra Hussaini (American Museum of Natural History), Susan Butts and Jessica Utrup (Yale Peabody Museum), and Jessica Cundiff (Harvard Museum of Comparative Zoology) for access to coral skeleton samples held in museum collections; Lisa Greer (Washington and Lee University) for the modern *Orbicella annularis* specimen; Saeed Kahn (UCLA) for assistance with XRD; Céline Liorzou (IUEM, France) and Yoan Germain (Ifremer, France) for technical assistance; Jordon Bright, Katherine Whitacre, and Darrell Kaufman (Northern Arizona University Amino Acid Geochronology Laboratory) for amino acid analysis; the SIMS facility at UCLA for assistance with scanning electron microscopy; and Claire Divola (UCLA) for assistance with data management. We thank the reviewers for their helpful comments.

## SUPPLEMENTARY MATERIAL

The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/feart.2021.643864/full#supplementary-material

## REFERENCES

Allison, N., Finch, A. A., Webster, J. M., and Clague, D. A. (2007). Palaeoenvironmental Records from Fossil Corals: the Effects of Submarine Diagenesis on Temperature and Climate Estimates. *Geochimica et Cosmochimica Acta*. 71, 4693-4703. doi:10.1016/j.gca.2007.07.026

Bada, J. L. (1985). Amino Acid Racemization Dating of Fossil Bones. *Annu. Rev. Earth Planet. Sci.* 13, 241-268. doi:10.1146/annurev.ea.13.050185.001325

Bada, J. L., Wang, X. S., and Hamilton, H. (1999). Preservation of Key Biomolecules in the Fossil Record: Current Knowledge and Future Challenges. *Phil. Trans. R. Soc. Lond. B*. 354, 77-87. doi:10.1098/rstb.1999.0361

Bai, L., Sheeley, S., and Sweedler, J. V. (2009). Analysis of Endogenous D-Amino Acid-Containing Peptides in Metazoa. *Bioanal. Rev.* 1, 7-24. doi:10.1007/s12566-009-0001-2

Barker, S., Greaves, M., and Elderfield, H. (2003). A Study of Cleaning Procedures Used for Foraminiferal Mg/Ca Paleothermometry. *Geochem. Geophys. Geosystems*. 4, 8407. doi:10.1029/2003gc000559

Bassler, R. S. (1950). *Faunal Lists and Descriptions of Paleozoic Corals*. Boulder, Colorado: Geological Society of America.

Brahmi, C., Kopp, C., Domart-Coulon, I., Stolarski, J., and Meibom, A. (2012). Skeletal Growth Dynamics Linked to Trace-Element Composition in the Scleractinian Coral *Pocillopora Damicornis*. *Geochimica et Cosmochimica Acta*. 99, 146-158. doi:10.1016/j.gca.2012.09.031

Brand, U., and Veizer, J. (1980). Chemical Diagenesis of a Multicomponent Carbonate System; 1, Trace Elements. *J. Sediment. Res.* 50, 1219-1236. doi:10.1306/212F7BB7-2B24-11D7-8648000102C1865D

Chung, F. H. (1974). Quantitative Interpretation of X-ray Diffraction Patterns of Mixtures. I. Matrix-flushing Method for Quantitative Multicomponent Analysis. *J. Appl. Cryst.* 7, 519-525. doi:10.1107/s0021889874010375

Cohen, A. L., and McConnaughey, T. A. (2003). "6. Geochemical Perspectives on Coral Mineralization," in *Biomineralization*. Editors P.M. Dove, S. Weiner, and J. Deyoreo, 151-188. doi:10.1515/9781501509346-011

Collins, M. J., Nielsen-Marsh, C. M., Hiller, J., Smith, C. I., Roberts, J. P., Prigodich, R. V., et al. (2002). The Survival of Organic Matter in Bone: a Review. *Archaeometry*. 44, 383-394. doi:10.1111/1475-4754.t01-1-00071

Cuif, J.-P., and Dauphin, Y. (1998). Microstructural and Physico-Chemical Characterization of 'centers of Calcification' in Septa of Some Recent Scleractinian Corals. *Paläontol. Z.* 72, 257-269. doi:10.1007/bf02988357

de Villiers, S., Shen, G. T., and Nelson, B. K. (1994). The -temperature Relationship in Coralline Aragonite: Influence of Variability in and Skeletal Growth Parameters. *Geochimica et Cosmochimica Acta* 58, 197-208. doi:10.1016/0016-7037(94)90457-x

DeCarlo, T. M., Gaetani, G. A., Holcomb, M., and Cohen, A. L. (2015). Experimental Determination of Factors Controlling U/Ca of Aragonite Precipitated from Seawater: Implications for Interpreting Coral Skeleton. *Geochimica et Cosmochimica Acta*. 162, 151-165. doi:10.1016/j.gca.2015.04.016

Demarchi, B. (2020). *Amino Acids and Proteins in Fossil Biominerals: An Introduction for Archaeologists and Palaeontologists*. New Jersey, United States: John Wiley & Sons. doi:10.1002/9781119089537

Drake, J. L., Mass, T., Stolarski, J., Von Euw, S., Schootbrugge, B., and Falkowski, P. G. (2020a). How Corals Made Rocks through the Ages. *Glob. Change Biol.* 26, 31-53. doi:10.1111/gcb.14912
Drake, J. L., Whitelegge, J. P., and Jacobs, D. K. (2020b). First Sequencing of Ancient Coral Skeletal Proteins. *Scientific Rep.* 10, 1–11. doi:10.1038/s41598-020-75846-4

Foster, L., Andersson, C., Høie, H., Allison, N., Finch, A., and Johansen, T. (2008). Effects of Micromilling on 618O in Biogenic Aragonite. *Geochem. Geophys. Geosystems.* 9. doi:10.1029/2007gc001911

Gabitov, R., Schmitt, A., Rosner, M., McKeegan, K., Gaetani, G. A., Cohen, A. L., et al. (2011). *In Situ* δ¹⁸Li, Li/Ca, and Mg/Ca Analyses of Synthetic Aragonites. *Geochem. Geophys. Geosystems* 12. doi:10.1029/2010gc003322

Gabitov, R., Sadekov, A., Yapaskurt, V., Borrelli, C., Bychkov, A., Sabourin, K., et al. (2019). Elemental Uptake by Calcite Slowly Grown from Seawater Solution: an Iin-Ssitu Study via Depth Profiling. *Front. Earth Sci.* 7, 51. doi:10.3389/feart.2019.00051

Genchi, G. (2017). An Overview on D-Amino Acids. *Amino Acids.* 49, 1521–1533. doi:10.1007/s00726-017-2459-5

Gill, I., Olson, J. J., and Hubbard, D. K. (1995). Corals, Paleotemperature Records, and the Aragonite-Calcite Transformation. *Geol.* 23, 333–336. doi:10.1130/0091-7613(1995)023<0333:cprata>2.3.co;2

Gothmann, A. M., Stolarski, J., Adkins, J. F., Schoene, B., Dennis, K. J., Schrag, D. P., et al. (2015). Fossil Corals as an Archive of Secular Variations in Seawater Chemistry since the Mesozoic. *Geochimica et Cosmochimica Acta.* 160, 188–208. doi:10.1016/j.gca.2015.03.018

Griffiths, N., Müller, W., Johnson, K. G., and Aguilera, O. A. (2013). Evaluation of the Effect of Diagenetic Cements on element/Ca Ratios in Aragonitic Early Miocene (~16Ma) Caribbean Corals: Implications for ‘deep-Time’ Palaeo-Environmental Reconstructions. *Palaeogeogr. Palaeoclimatol. Palaeoecol.* 369, 185–200. doi:10.1016/j.palaeo.2012.10.018

Guillermic, M., Misra, S., Eagle, R., Villa, A., Chang, F., and Tripati, A. (2020). Seawater pH Reconstruction Using boron Isotopes in Multiple Planktonic Foraminifera Species with Different Depth Habitats and Their Potential to Constrain pH and pCO2 Gradients. *Biogeosciences.* 17, 3487–3510. doi:10.5194/bg-17-3487-2020

Hammer, Ø., Harper, D. A., and Ryan, P. D. (2001). PAST: Paleontological Statistics Software Package for Education and Data Analysis. *Palaeocontol. electronica.* 4, 9. Available at: http://palaeo-electronica.org/2001_1/past/issue1_01.htm

Hare, P. (1974). *Amino Acid Dating of Bone—The Influence of Water*. Washington, DC: Carnegie Institute of Washington Year Book, 73. 576–581.

Hare, P. (1980). "Organic Geochemistry of Bone and its Relation to the Survival of Bone in the Natural Environment," in *Fossils in the Making: Vertebrate Taphonomy and Paleoeology*. Editors AK Behrensmeyer and AP Hill (Chicago: University of Chicago Press).

Hare, P. E. (1962). *The Amino Acid Composition of the Organic Matrix of Some Recent and Fossil Shells of Some West Coast Species of Mytilus*. Dissertation: California Institute of Technology

Hathorne, E. C., Gagnon, A., Felis, T., Adkins, J., Asami, R., Boer, W., et al. (2013). Interlaboratory Study for Coral Sr/Ca and Other element/Ca Ratio Measurements. *Geochem. Geophys. Geosyst.* 14, 3730–3750. doi:10.1002/ggge.20230

Hendy, A., Walker, L., and Mertz, W. (2020). LACM Invertebrate Paleontology. California: Natural History Museum of Los Angeles County.

Hendy, E., Gagan, M., Lough, J., McCulloch, M., and DeMenocal, P. (2007). Impact of Skeletal Dissolution and Secondary Aragonite on Trace Element and Isotopic Climate Proxies in *Porites* Corals. *Paleoceanography.* 22. doi:10.1029/2007pa001462

Kaufman, D. S. (2000). 12. Amino Acid Racemization in Ostracodes. *Perspectives in Amino Acid and Protein Geochemistry*, 145.

Kaufman, D. (2003). Dating Deep-lake Sediments by Using Amino Acid Racemization in Fossil Ostracodes. *Geol.* 31, 1049–1052. doi:10.1130/g20004.1

Kaufman, D. (2006). Temperature Sensitivity of Aspartic and Glutamic Acid Racemization in the Foraminifera Pulleniatina. *Quat. Geochronol.* 1, 188–207. doi:10.1016/j.quageo.2006.06.008

Kaufman, D. S., and Manley, W. F. (1998). A New Procedure for Determining DL Amino Acid Ratios in Fossils Using Reverse Phase Liquid Chromatography. *Quat. Sci. Rev.* 17, 987–1000. doi:10.1016/s0277-3791(97)00086-3

Mass, T., Drake, J. L., Haramaty, L., Rosenthal, Y., Schofield, O. M. E., Sherrell, R. M., et al. (2012). Aragonite Precipitation by “Proto-polyps” in

Coral Cell Cultures. PLoS ONE. 7, e35049. doi:10.1371/journal.pone.0035049

Mass, T., Drake, J. L., Peters, E. C., Jiang, W., and Falkowski, P. G. (2014). Immunolocalization of Skeletal Matrix Proteins in Tissue and mineral of the Coral *Stylophora Pistillata*. *Proc. Natl. Acad. Sci.* 111, 12728–12733. doi:10.1073/pnas.1408621111

Mass, T., Giuffre, A. J., Sun, C.-Y., Stifler, C. A., Frazier, M. J., Neder, M., et al. (2017). Amorphous Calcium Carbonate Particles Form Coral Skeletons. *Proc. Natl. Acad. Sci. USA.* 114, E7670–E7678. doi:10.1073/pnas.1707890114

Mavromatis, V., Goetschl, K. E., Grengg, C., Konrad, F., Purgstaller, B., and Dietzel, M. (2018). Barium Partitioning in Calcite and Aragonite as a Function of Growth Rate. *Geochimica et Cosmochimica Acta.* 237, 65–78. doi:10.1016/j.gca.2018.06.018

Mavromatis, V., Montouillout, V., Noireaux, J., Gaillardet, J., and Schott, J. (2015). Characterization of boron Incorporation and Speciation in Calcite and Aragonite from Co-precipitation Experiments under Controlled pH, Temperature and Precipitation Rate. *Geochimica et Cosmochimica Acta.* 150, 299–313. doi:10.1016/j.gca.2014.10.024

McCobb, L. M. E., Briggs, D. E. G., Hall, A. R., and Kenward, H. K. (2004). The Preservation of Invertebrates in 16th-Century Cesspits at St Saviourgate, York. *Archaeometry.* 46, 157–169. doi:10.1111/j.1475-4754.2004.00150.x

McGregor, H. V., and Gagan, M. K. (2003). Diagenesis and Geochemistry of Porites Corals from Papua New Guinea. *Geochimica et Cosmochimica Acta.* 67, 2147–2156. doi:10.1016/s0016-7037(02)01050-5

Meibom, A., Cuif, J.-P., Houlbreque, F., Mostefaoui, S., Dauphin, Y., Meibom, K. L., et al. (2008). Compositional Variations at Ultra-structure Length Scales in Coral Skeleton. *Geochimica et Cosmochimica Acta.* 72, 1555–1569. doi:10.1016/j.gca.2008.01.009

Meibom, A., Yurimoto, H., Cuif, J. P., Domart-Coulon, I., Houlbreque, F., Constantz, B., et al. (2006). Vital Effects in Coral Skeletal Composition Display Strict Three-dimensional Control. *Geophys. Res. Lett.* 33. doi:10.1029/2006gl025968

Menadakis, M., Maroulis, G., and Koutsoukos, P. G. (2009). Incorporation of Mg2+, Sr2+, Ba2+ and Zn2+ into Aragonite and Comparison with Calcite. *J. Math. Chem.* 46, 484–491. doi:10.1007/s10910-008-9490-4

Misra, S., Greaves, M., Owen, R., Kerr, J., Elmore, A. C., and Elderfield, H. (2014). Determination of B/Ca of Natural Carbonates by HR-ICP-MS. *Geochem. Geophys. Geosyst.* 15, 1617–1628. doi:10.1002/2013gc005049

Mitterer, R. M. (1993). *The Diagenesis of Proteins and Amino Acids in Fossil Shells. Organic Geochemistry*. Berlin, Germany: Springer, 739–753. doi:10.1007/978-1-4615-2890-6_35

Muhs, D. R., Simmons, K. R., Schumann, R. R., and Halley, R. B. (2011). Sea-level History of the Past Two Interglacial Periods: New Evidence from U-Series Dating of Reef Corals from South Florida. *Quat. Sci. Rev.* 30, 570–590. doi:10.1016/j.quascirev.2010.12.019

Multer, H. G., Gischler, E., Lundberg, J., Simmons, K. R., and Shinn, E. A. (2002). Key Largo limestone Revisited: Pleistocene Shelf-Edge Facies, Florida Keys, USA. *Facies.* 46, 229–271. doi:10.1007/bf02668083

Muscatine, L., Goiran, C., Land, L., Jaubert, J., Cuif, J.-P., and Allemand, D. (2005). Stable Isotopes (13C and 15N) of Organic Matrix from Coral Skeleton. *Proc. Natl. Acad. Sci.* 102, 1525–1530. doi:10.1073/pnas.0408921102

Neuberger, A. (1948). *Stereochemistry of Amino Acids. Advances in Protein Chemistry*. Amsterdam, Netherlands: Elsevier, 297–383. doi:10.1016/s0065-3233(08)00009-1

Nothdurft, L. D., Webb, G. E., Bostrom, T., and Rintoul, L. (2007). Calcite-filled Borings in the Most Recently Deposited Skeleton in Live-Collected *Porites* (Scleractinia): Implications for Trace Element Archives. *Geochimica et Cosmochimica Acta.* 71, 5423–5438. doi:10.1016/j.gca.2007.09.025

Nothdurft, L. D., and Webb, G. E. (2009). Earliest Diagenesis in Scleractinian Coral Skeletons: Implications for Palaeoclimate-Sensitive Geochemical Archives. *Facies.* 55, 161–201. doi:10.1007/s10347-008-0167-z

Painter, T. J. (2003). "Sphagnum-dominated Peat Bogs," in *Paleobiology II*. Editors D.E.G. Briggs and P.R. Crowther (Malden, MA: Blackwell Science), 321–325.

Peled, Y., Drake, J. L., Malik, A., Almuly, R., Lalzar, M., Morgenstern, D., et al. (2020). Optimization of Skeletal Protein Preparation for LC-MS/MS Sequencing Yields Additional Coral Skeletal Proteins in *Stylophora Pistillata*. *BMC Mater.* 2, 8. doi:10.1186/s42833-020-00014-x
Penkman, K. E. H., Kaufman, D. S., Maddy, D., and Collins, M. J. (2008). Closed-system Behaviour of the Intra-crystalline Fraction of Amino Acids in Mollusc Shells. *Quat. Geochronol.* 3, 2–25. doi:10.1016/j.quageo.2007.07.001

Puverel, S., Tambutt, E., Zoccola, D., Domart-Coulon, I., Bouchot, A., Lotto, S. v., et al. (2005). Antibodies against the Organic Matrix in Scleractinians: A New Tool to Study Coral Biomineralization. *Coral Reefs* 24, 149–156. doi:10.1007/s00338-004-0456-0

Quinn, T. M., and Sampson, D. E. (2002). A Multiproxy Approach to Reconstructing Sea Surface Conditions Using Coral Skeleton Geochemistry. *Paleoceanography.* 17, 1–14. doi:10.1029/2000pa000528

Rabier, C., Anguy, Y., Cabioch, G., and Genthon, P. (2008). Characterization of Various Stages of Calcitization in Porites Sp Corals from Uplifted Reefs - Case Studies from New Caledonia, Vanuatu, and Futuna (South-West Pacific). *Sediment. Geology.* 211, 73–86. doi:10.1016/j.sedgeo.2008.08.005

Rollion-Bard, C., and Blamart, D. (2015). Possible Controls on Li, Na, and Mg Incorporation into Aragonite Coral Skeletons. *Chem. Geology.* 396, 98–111. doi:10.1016/j.chemgeo.2014.12.011

Sakalauskaite, J., Andersen, S. H., Biagi, P., Borrello, M. A., Cocquerez, T., Colonese, A. C., et al. (2019). Palaeoshellomics' Reveals the Use of Freshwater Mother-Of-Pearl in Prehistory. *eLife.* 8, e45644. doi:10.7554/elife.45644

Sakalauskaite, J., Marin, F., Pergolizzi, B., and Demarchi, B. (2020). Shell Palaeoproteomics: First Application of Peptide Mass Fingerprinting for the Rapid Identification of Mollusc Shells in Archaeology. *J. Proteomics.* 227, 103920. doi:10.1016/j.jprot.2020.103920

Sayani, H. R., Cobb, K. M., Cohen, A. L., Elliott, W. C., Nurhati, I. S., Dunbar, R. B., et al. (2011). Effects of Diagenesis on Paleoclimate Reconstructions from Modern and Young Fossil Corals. *Geochimica et Cosmochimica Acta.* 75, 6361–6373. doi:10.1016/j.gca.2011.08.026

Schroeter, E. R., DeHart, C. J., Cleland, T. P., Zheng, W., Thomas, P. M., Kelleher, N. L., et al. (2017). Expansion for the *Brachylophosaurus canadensis* Collagen I Sequence and Additional Evidence of the Preservation of Cretaceous Protein. *J. Proteome Res.* 16, 920–932. doi:10.1021/acs.jproteome.6b00873

Shen, G. T., Campbell, T. M., Dunbar, R. B., Wellington, G. M., Colgan, M. W., and Glynn, P. W. (1991). Paleochemistry of Manganese in Corals from the Galapagos Islands. *Coral Reefs.* 10, 91–100. doi:10.1007/bf00571827

Stoll, H. M., Ruiz Encinar, J., Ignacio Garcia Alonso, J., Rosenthal, Y., Probert, I., and Klaas, C. (2001). A First Look at Paleotemperature Prospects from Mg in Coccolith Carbonate: Cleaning Techniques and Culture Measurements. *Geochem. Geophys. Geosystems.* 2, (5). doi:10.1029/2000gc000144

Swart, P. K., Elderfield, H., and Greaves, M. J. (2002). A High-Resolution Calibration of Sr/Ca Thermometry Using the Caribbean coralMontastraea Annularis. *Geochem.-Geophys.-Geosyst.* 3, 1–11. doi:10.1029/2002gc000306

Team, R. (2019). *RStudio: Integrated Development for R.* Boston, MA: RStudio, Inc.

Tomiak, P. J., Andersen, M. B., Hendy, E. J., Potter, E. K., Johnson, K. G., and Penkman, K. E. H. (2016). The Role of Skeletal Micro-architecture in Diagenesis and Dating of *Acropora Palmata*. *Geochimica et Cosmochimica Acta.* 183, 153–175. doi:10.1016/j.gca.2016.03.030

Tomiak, P. J., Penkman, K. E. H., Hendy, E. J., Demarchi, B., Murrells, S., Davis, S. A., et al. (2013). Testing the Limitations of Artificial Protein Degradation Kinetics Using Known-Age Massive *Porites* Coral Skeletons. *Quat. Geochronol.* 16, 87–109. doi:10.1016/j.quageo.2012.07.001

Tornabene, C., Martindale, R. C., Wang, X. T., and Schaller, M. F. (2017). Detecting Photosymbiosis in Fossil Scleractinian Corals. *Scientific Rep.* 7, 9465. doi:10.1038/s41598-017-09008-4

Tuross, N., Behrensmeyer, A. K., Eanes, E. D., Fisher, L. W., and Hare, P. E. (1989). Molecular Preservation and Crystallographic Alterations in a Weathering Sequence of Wildebeest Bones. *Appl. Geochem.* 4, 261–270. doi:10.1016/0883-2927(89)90027-9

Wainwright, S. A. (1963). Skeletal Organization in the Coral, *Pocillopora Damicornis*. *Q. J. Microscopical Sci.* s3-104, 169-183. doi:10.1242/jcs.s3-104.66.169

Waite, A. J., and Swart, P. K. (2015). The Inversion of Aragonite to Calcite during the Sampling of Skeletal Archives: Implications for Proxy Interpretation. *Rapid Commun. Mass. Spectrom.* 29, 955-964. doi:10.1002/rcm.7180

Wang, X. T., Sigman, D. M., Cohen, A. L., Sinclair, D. J., Sherrell, R. M., Weigand, M. A., et al. (2015). Isotopic Composition of Skeleton-Bound Organic Nitrogen in Reef-Building Symbiotic Corals: a New Method and Proxy Evaluation at Bermuda. *Geochimica et Cosmochimica Acta.* 148, 179-190. doi:10.1016/j.gca.2014.09.017

Watanabe, T., Winter, A., and Oba, T. (2001). Seasonal Changes in Sea Surface Temperature and Salinity during the Little Ice Age in the Caribbean Sea Deduced from Mg/Ca and 180/160 Ratios in Corals. *Mar. Geology.* 173, 21–35. doi:10.1016/s0025-3227(00)00166-3

Young, S. D. (1971). Organic Material from Scleractinian Coral Skeletons-I. Variation in Composition between Several Species. *Comp. Biochem. Physiol. B: Comp. Biochem.* 40, 113-120. doi:10.1016/0305-0491(71)90067-8

Yu, J., Day, J., Greaves, M., and Elderfield, H. (2005). Determination of Multiple Element/calcium Ratios in Foraminiferal Calcite by Quadrupole ICP-MS. *Geochem. Geophys. Geosystems.* 6, (8). doi:10.1029/2005gc000964

Zoology, H. M. o. C. (2020). MCZ Kronosaurus Logo MCZBASE:The Database of the Zoological Collections. Museum of Comparative Zoology - Harvard University.

**Conflict of Interest:** The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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