# Heat adaptation in pigs: phenotypes, genotypes & blood transcriptomics in the PigHeaT program

Guillaume Devailly, Yann Labrune, Katia Feve, Laure Gress, Jean-Luc Gourdine, Hélène Gilbert, David Renaudeau, Juliette Riquet

## ► To cite this version:

Guillaume Devailly, Yann Labrune, Katia Feve, Laure Gress, Jean-Luc Gourdine, et al. Heat adaptation in pigs: phenotypes, genotypes & blood transcriptomics in the PigHeaT program. International Conference of System Biology (ICSB), Oct 2018, Lyon, France. hal-01929027

HAL Id: hal-01929027

https://hal.science/hal-01929027

Submitted on 5 Jun 2020

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INRA
SCIENCE & IMPACT

AGENCE NATIONALE DE LA RECHERCHE
ANR

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# Heat adaptation in pigs:
phenotypes, genotypes & blood transcriptomics
in the PigHeaT program

Guillaume Devailly, Yann Labrune, Katia Feve, Laure Gress, Jean-Luc Gourdine, Hélène Gilbert, David Renaudeau, Juliette Riquet

## Abstract & experimental setup

Heat wave frequency is increasing due to global warming, resulting in consequential impacts on agricultural production systems. In temperate production pigs, heat waves reduce production performances and cause animal discomforts. Improving heat resistance in pigs is therefore needed to mitigate the effects of global warming.

Caribbean Creole pigs are well adapted to tropical climate, but show low production values, the exact opposite of Large White pigs. Animals from **a back-cross of Large White and Creole pigs** were raised in experimental units of INRA in **both tropical and temperate climate**. Animals in temperate climate were further challenged with a three weeks-long artificial heat wave. Production traits and thermo-regulatory responses were recorded and associated with their genotypes. Blood samples of a subset of animals were used for metabolomics and transcriptomics analyses in both environments, and throughout the induced heat stress (N=180 per conditions).

<figure><img src="image_4.png" /><figcaption>Temperate climate</figcaption></figure>

For back-crossed animals, we have:

- phenotypes for production & thermo-regulation traits

- genotypes (60k markers, Illumina PorcineSNP60v2)

- blood transcriptome (Agilent SurePrint G3 Pig GE 8x60K arrays)

- blood metabolome (1HNMR)

Published studies on this experimental setup:

- quantitative genetics:

Rosé et al. *J Anim Sci.* 2017

- blood metabolic biomarkers of heat sensitivity:

Dou et al. *PLoS One* 2017

## Results

<figure><img src="image_5.png" /><figcaption>### eQTL detection & visualisation</figcaption></figure>

<figure><img src="image_6.png" /><figcaption>Variants associated with (every) gene expression level</figcaption></figure>

<caption>### Gene expression level in blood as a biomarker for heat wave response?</caption>

Pigs grown in temperate climate were submitted to an artificial heat wave during 3 weeks.
We looked for genes with an **expression level before the stress correlated with a physiological response to the stress** (here, body temperature increase after 48h at 30°C)

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<figure><img src="image_8.png" /><figcaption>### Impact of genetic × environment interactions on gene expression</figcaption></figure>

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## Conclusions & Perspectives

- Effect of the climate on the blood transcriptome

- eQTL detection will allow better phenotypic QTL annotation

- GxE interactions in transcription regulation

- Blood transcripts as biomarkers for heat response?

→ QTL detection for transcriptomic changes under heat stress

→ variants & transcripts associated with heat tolerance & resistance

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INRA
SCIENCE & IMPACT

* guillaume.devailly@inra.fr
  twitter: @G_Devailly
  GenEpi @ GenPhySE
  genphyse.toulouse.inra.fr/groups/genepi

24, chemin de Borde-Rouge -
Auzeville Tolosane
31326 Castanet Tolosan

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