Phthiocerol Dimycocerosates From Mycobacterium
tuberculosis Increase the Membrane Activity of
Bacterial Effectors and Host Receptors

Jacques Augenstreich, Evert Haanappel, Fadel Sayes, Roxane Simeone, Valerie Guillet, Serge Mazeres, Christian Chalut, Lionel Mourey, Roland Brosch, Christophe Guilhot, et al.

► To cite this version:

Jacques Augenstreich, Evert Haanappel, Fadel Sayes, Roxane Simeone, Valerie Guillet, et al. Phthio-
cerol Dimycocerosates From Mycobacterium tuberculosis Increase the Membrane Activity of Bacterial
Effectors and Host Receptors. Frontiers in Cellular and Infection Microbiology, 2020, 10, pp.420.
10.3389/fcimb.2020.00420. pasteur-02873531

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# Phthiocerol Dimycocerosates From *Mycobacterium tuberculosis* Increase the Membrane Activity of Bacterial Effectors and Host Receptors

Jacques Augenstreich¹†, Evert Haanappel¹†, Fadel Sayes², Roxane Simeone²,
Valérie Guillet¹, Serge Mazeres¹, Christian Chalut¹, Lionel Mourey¹, Roland Brosch²,
Christophe Guilhot¹* and Catherine Astarie-Dequeker¹*

OPEN ACCESS

**Edited by:**
Patricia Ann Champion,
University of Notre Dame,
United States

**Reviewed by:**
Jianjun Sun,
The University of Texas at El Paso,
United States
Jessica Seeliger,
Stony Brook University, United States

*Correspondence:
Christophe Guilhot
christophe.guilhot@ipbs.fr
Catherine Astarie-Dequeker
catherine.astarie-dequeker@ipbs.fr

**Specialty section:**
This article was submitted to
*Bacteria and Host*,
a section of the journal
Frontiers in Cellular and Infection
Microbiology

Received: 08 May 2020

Accepted: 08 July 2020

Published: 14 August 2020

**Citation:**
Augenstreich J, Haanappel E, Sayes F, Simeone R, Guillet V, Mazeres S, Chalut C, Mourey L, Brosch R, Guilhot C and Astarie-Dequeker C (2020) Phthiocerol Dimycocerosates From *Mycobacterium tuberculosis* Increase the Membrane Activity of Bacterial Effectors and Host Receptors. *Front. Cell. Infect. Microbiol.* 10:420. doi: 10.3389/fcimb.2020.00420

*Mycobacterium tuberculosis* (*Mtb*) synthesizes a variety of atypical lipids that are exposed at the cell surface and help the bacterium infect macrophages and escape elimination by the cell's immune responses. In the present study, we investigate the mechanism of action of one family of hydrophobic lipids, the phthiocerol dimycocerosates (DIM/PDIM), major lipid virulence factors. DIM are transferred from the envelope of *Mtb* to host membranes during infection. Using the polarity-sensitive fluorophore C-Laurdan, we visualize that DIM decrease the membrane polarity of a supported lipid bilayer put in contact with mycobacteria, even beyond the site of contact. We observed that DIM activate the complement receptor 3, a predominant receptor for phagocytosis of *Mtb* by macrophages. DIM also increased the activity of membrane-permeabilizing effectors of *Mtb*, among which the virulence factor EsxA. This is consistent with previous observations that DIM help *Mtb* disrupt host cell membranes. Taken together, our data show that transferred DIM spread within the target membrane, modify its physical properties and increase the activity of host cell receptors and bacterial effectors, diverting in a non-specific manner host cell functions. We therefore bring new insight into the molecular mechanisms by which DIM increase *Mtb*'s capability to escape the cell's immune responses.

**Keywords:** macrophages, *Mycobacterium tuberculosis*, phthiocerol dimycocerosates, EsxA, membranolytic activity, receptors, membranes, complement receptor 3

## INTRODUCTION

The envelope lipids, phthiocerol dimycocerosates (DIM/PDIM) are among the major virulence factors of *Mycobacterium tuberculosis* (*Mtb*). Apart from their structural role as components of the mycobacterial envelope, DIM are harnessed by the bacteria to manipulate host immune functions, especially during the early steps of infection, when *Mtb* encounters macrophages (Rousseau et al., 2004; Astarie-Dequeker et al., 2009; Cambier et al., 2014; Passemar et al., 2014; Barczak et al., 2017; Quigley et al., 2017). However, there is still a great deal of uncertainty about the molecular mechanisms of DIM-mediated effects in host cells.

<footnote>† Institut de Pharmacologie et de Biologie Structurale (IPBS), Université de Toulouse, CNRS-UPS UMR 5089, Toulouse, France, ² Institut Pasteur, Unit for Integrated Mycobacterial Pathogenomics, CNRS UMR3525, Paris, France</footnote>

<footnote>† These authors have contributed
equally to this work</footnote>
Our group has reported that DIM are transferred from the mycobacterial envelope to the membranes of host macrophages, and that this transfer increases the phagocytosis of bacteria (Augenstreich et al., 2019). Further investigations indicated that once inserted in the host membrane, DIM adopt a conical shape which disturbs the organization of membrane lipids (Augenstreich et al., 2019). We hypothesize that this has functional consequences for the activity of membrane-associated contributors to phagocytosis. Phagocytosis occurs through the recognition of Pathogen-Associated Molecular Patterns (PAMPs) present at the bacterial surface by receptors expressed in the host cell plasma membrane. In the case of *Mtb*, phagocytosis involves multiple receptors, including the complement receptor 3 (CR3, also named CD11b/CD18 or Mac-1) (Schlesinger, 1993; Stokes et al., 1993; Cywes et al., 1996). Interestingly, blocking CR3 with antibodies significantly reduces the entry of a DIM-proficient *Mtb* strain into macrophages without affecting the uptake of a DIM-deficient mutant (Astarie-Dequeker et al., 2009), suggesting that the disorganizing effects of DIM on host cell membranes could target the activity of CR3.

DIM also cause membrane damage, such as phagosomal rupture and cell death (Augenstreich et al., 2017; Quigley et al., 2017). Previous work has shown that DIM act in concert with the type VII secretion system ESX-1 (T7SS/ESX-1), in a manner that involves the 6-kDa early secreted antigenic target (ESAT-6, also known as EsxA) (Augenstreich et al., 2017; Barczak et al., 2017). EsxA has been predicted to insert into membranes and to lyse artificial and biological membranes (Hsu et al., 2003; Gao et al., 2004; De Jonge et al., 2007; Smith et al., 2008; Ma et al., 2015; Peng et al., 2016). Interestingly, the membranolytic activity of EsxA was found to depend on the lipid composition and on membrane fluidity (Augenstreich et al., 2017; Ray et al., 2019). The mechanism by which DIM and EsxA collaborate remains unclear. Barczak and colleagues proposed that the secretion of EsxA requires DIM (Barczak et al., 2017). However, previous findings indicated that the DIM-deficient mycobacterial strains, which are affected in their membranolytic activity, secrete similar amounts of EsxA as the parental strains (Augenstreich et al., 2017; Quigley et al., 2017). Recently, our group reported that the incorporation of DIM in the membrane of model liposomes enhanced EsxA activity (Augenstreich et al., 2017). Nevertheless, the origin of the membrane-disrupting activity of DIM remains obscure. Indeed, their potentiating effect on EsxA's activity is independent of their ability to rigidify membranes and is limited to membranes containing lipids extracted from macrophages (Augenstreich et al., 2017). Moreover, the membranolytic activity of EsxA itself was called into question by Conrad and colleagues who showed that the hemolytic activity of recombinant EsxA obtained from the same source was due to residual detergent used in the purification protocol (Conrad et al., 2017). Thus, the capacity of *Mtb* to permeabilize host membranes is more complex than initially thought.

It therefore remains of importance to determine how DIM contribute to the phagocytosis of *Mtb* and to the membranolytic activity of bacteria at the molecular level.

# MATERIALS AND METHODS

## Antibodies and Reagents

The 2LPM19c mouse antibody directed against the CD11b subunit of human CR3 (IgG1, dilution 1:20) and normal mouse IgG1 were purchased from Santa Cruz Biotechnology. The CBRM1/5 mouse antibody raised against an activation-specific epitope on the CD11b subunit of human CR3 (IgG1, dilution 1:50) was from eBiosciences. POPC (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine) was from Avanti Polar Lipids. THP-1 lipids were extracted from THP-1 cells as described previously (Augenstreich et al., 2017). C-Laurdan was synthesized as described in Mazeres et al. (2014). DIM (average $m_w$ = 1,432 g/mol) were purified from *Mycobacterium canetti* (Augenstreich et al., 2019). Stock solutions of 40 mg/mL were prepared by dissolving the dried lipids in chloroform: methanol (2:1, vol/vol) (Augenstreich et al., 2019). For cell treatment, DIM were dispersed in culture medium at a final concentration of 0.1 mg/mL corresponding to 70 µM. The other reagents were purchased from Sigma-Aldrich.

## Bacterial Strains and Growth Conditions

The unmarked *mas* mutant (H37RvΔ*mas*), the *ppsE* mutant (H37RvΔ*ppsE*), the *esxA* mutant (H37RvΔ*esxA*) of *Mtb* and the *mas* mutant (BCGΔ*mas*), the spontaneous *fad26* mutant (BCGΔ*fad26*), the *pks15/1* mutant (BCGΔ*pks15/1*) of *Mycobacterium bovis* BCG were characterized in **Figure S1a** and in previous work (Constant et al., 2002; Astarie-Dequeker et al., 2009; Simeone et al., 2010; Augenstreich et al., 2017). The recombinant BCG strains complemented with the ESX-1 system in DIM-proficient or DIM-deficient strains (BCG::ESX-1 and BCGΔ*mas*::ESX-1) were constructed as previously mentioned (Augenstreich et al., 2017). Bacteria were rendered fluorescent by transferring the plasmid pMV361H *gfp* (Astarie-Dequeker et al., 2009) or the plasmid pMVmCherry derived from a plasmid pMV361eH harboring the mCherry encoding gene (Burbaud et al., 2016). All strains were cultured at 37°C as previously described (Astarie-Dequeker et al., 2009; Augenstreich et al., 2017).

## Phagocyte Culture

The human promonocytic cell line THP-1 (ECACC 88081201; Salisbury, UK) was cultured and differentiated in macrophages with PMA, as mentioned in Augenstreich et al. (2019). Human blood purchased from the Etablissement Français du Sang (Toulouse, France) was collected from fully-anonymous non-tuberculous donors. Peripheral blood mononuclear cells and human monocyte-derived macrophages (hMDM) were prepared as previously described (Astarie-Dequeker et al., 2009).

## Phagocytosis Assay

Single bacteria suspensions were prepared from exponentially growing strains as previously described (Astarie-Dequeker et al., 2009; Tabouret et al., 2010). The concentration of bacteria was estimated from the optical density at 600 nm ($OD_{600}$ = 0.1 for 2 × 10⁷ bacilli/mL). hMDM cultured in RPMI on glass coverslips were incubated at 37°C for 1 h with GFP-expressing bacteria at a multiplicity-of-infection (MOI) of 10:1 or with zymosan at MOI
30:1. Phagocytosis was assessed by fluorescence microscopy as previously reported (Astarie-Dequeker et al., 2009; Augenstreich et al., 2019).

## Expression and Purification of Recombinant EsxA

Recombinant Mtb EsxA (rEsxA) was prepared according to the step-by-step protocol provided by BEI Resources (available at csu-cvmbs.colostate.edu/Documents/dobos-rp004.pdf). The C-terminally His-tagged protein was expressed in Escherichia coli BL21(DE3)pLysS using the plasmid pMRLB.7 containing Mtb esxA (NR-50170, BEI Resources). rEsxA was then purified according to the BEI protocol, adapted by omitting the washing step with ASB-14. The protein was checked for purity by SDS/PAGE followed by Coomassie staining, and dialyzed using 3,500-MWCO dialysis tubing in 10 mM ammonium bicarbonate, lyophilized and stored at -20°C. For the calcein leakage experiments, a small amount of rEsxA was weighed and solubilized in PBS at a final concentration of 2 mg/mL.

## Assay for Measurement of Membrane Polarity

We characterized membrane polarity using the membrane dye C-Laurdan, whose fluorescence spectrum shifts toward lower wavelengths when the membrane becomes more apolar (Mazeres et al., 2017). A supported lipid membrane of phospholipid POPC labeled with 1% C-Laurdan was formed on a Ø25 mm microscope coverslip using the method of vesicle fusion (Mascalchi et al., 2012). The membrane was put in contact with a suspension of $2 \times 10^6$ bacteria, centrifuged at 1,500 g for 5 min at room temperature to sediment the bacteria onto the membrane, and further incubated for 20 min at room temperature. We took two-photon microscopy images on a Zeiss LSM710 inverted confocal microscope using a 40× water immersion objective. The membrane polarity was characterized by the Generalized Polarization (GP), a ratiometric measure given by $GP = (I_{440} - I_{490})/(I_{440} + I_{490})$ where $I_{440}$ and $I_{490}$ are the fluorescence intensities of C-Laurdan at 440 and 490 nm, respectively.

## Calcein Leakage Assays

The membranolytic activity of compounds was evaluated on liposomes using a calcein leakage assay, as previously described (Augenstreich et al., 2017). The liposomes were composed of POPC or of THP-1 lipids supplemented or not with 10% (mol/mol) of DIM and contained a solution of 50 mM calcein.

## Contact Dependent Hemolytic Activity of Mycobacterium spp

Lytic activity of Mycobacterium spp was detected by a hemolysis assay. Exponentially-growing mycobacteria in 7H9-ADC-Tween 80 0.05% were pelleted by centrifugation and washed twice in PBS (Smith et al., 2008; Conrad et al., 2017). Fresh blood was centrifuged and the pellet was resuspended in isotonic NaCl solution and washed twice with PBS. The final red blood cells (RBC) suspension containing $1 \times 10^7$ cells was mixed with bacteria at MOI of 50:1, centrifuged and incubated at 37°C for 48 h. The pellet was resuspended, centrifuged and

the supernatant was recovered. The absorbance at 415 nm (A$_{415}$) was measured on a CLARIOstar 96-well microplate reader. The supernatants of cells treated with PBS or lysed with 0.1% Triton X-100 were used as reference values for 0 and 100% hemolysis, respectively. The percentage of hemolysis was calculated using the formula: % hemolysis = $(A_{415,sample} - A_{415,PBS})/(A_{415,TritonX100} - A_{415,PBS})$.

## Statistics

All results are expressed as mean ± standard error of the mean (SEM) for the indicated number of experiments (n). Statistical analyses were performed using GraphPad Prism 6.0 (GraphPad Software Inc.) and presented in figure legends. Differences were considered significant if $p < 0.05$.

# RESULTS

## DIM Contribute to the Membrane Polarity Decrease in a Lipid Bilayer Beyond the Site of Interaction With Mycobacteria

We previously reported that DIM are transferred from M. bovis BCG to the macrophage (Augenstreich et al., 2019) and decrease its overall membrane polarity (Astarie-Dequeker et al., 2009). We investigated whether this decrease is restricted to the site of contact between the bacilli and the host membrane, or extends beyond it, as the pleiotropic effects of DIM suggest. A supported POPC membrane labeled with the polarity-sensitive fluorophore C-Laurdan was put in contact with mCherry-expressing bacilli, either wild-type M. bovis BCG (BCG WT) or a DIM-deficient isogenic M. bovis BCG mutant, BCG$\Delta mas$, with a deleted *mas*, encoding an enzyme essential for DIM synthesis (Azad et al., 1996). POPC is a physiologically representative lipid forming fluid membranes at room temperature. Using mCherry fluorescence and transmission images, we first searched for bacteria immobilized on the membrane (Figure 1A, left). The majority of bacteria remained in suspension and diffused in the buffer (blurred spots in Figure 1A). By two-photon microscopy, we then imaged the C-Laurdan around immobile bacteria. Bacteria absorbed C-Laurdan from the bilayer (Figure 1A, right), implying that the C-Laurdan fluorescence spectrum at the point of contact contained contributions both from the membrane and the bacteria. We discerned no difference in C-Laurdan fluorescence intensity between the two strains (Figure S1b). We enlarged the bacterial contour, determined from the C-Laurdan fluorescence, three times by 5 pixels (520 nm), thus defining three concentric bands around the immobile bacterium (C1, C2, and C3 in Figure 1B image). From the average fluorescence spectrum in these bands, we calculated the corresponding Generalized Polarization (GP) values to characterize the membrane polarity in each band. The value of GP increases when the membrane becomes more apolar. We subtracted the intrinsic GP of the membrane, averaged over three zones outside C1, C2, and C3 and far from all bacteria, from the GP of the different bands to quantify the GP difference ($\Delta GP$) around bacteria. GP was increased inside the bacterial contour for both strains, reflecting the apolar nature of the mycobacterial envelope. We noticed
<figure><img src="image_3.png" /></figure>

**FIGURE 1** | Analysis of DIM-induced changes in membrane polarity of supported bilayers. A POPC bilayer labeled with C-Laurdan was formed on a glass coverslip and incubated with 2 × 10⁶ M. bovis BCG::mCherry or BCGΔ*mas*::mCherry for 20 min. Two-photon microscopy images were taken on an inverted confocal microscope equipped with a femto second pulsed laser (Chameleon Vision II) and with a 561 nm DPSS laser for mCherry fluorescence and transmission imaging. A build-in spectrometer allowed the acquisition of a fluorescence spectrum in each pixel. C-Laurdan was excited at 720 nm; its fluorescence spectrum was collected in 18 channels between 418 and 593 nm (channel width 9.7 nm), resulting in a λ-stack of 18 images. The acquisition time per image was 31 s (A) Immobilized bacteria were first selected by mCherry and transmission microscopy (left panel), then the C-Laurdan spectrum was acquired around an immobile bacterium by two-photon microscopy (right panel). (B) (left panel) Contour and concentric bands around an immobile bacterium. ΔGP was calculated in these bands using the average GP of three arbitrary zones (yellow circles) selected far from C1-3 and any bacteria as the reference GP value of the membrane in each picture. (Right panel) Individual and average ΔGP values for BCG and BCGΔ*mas*. Each symbol in the vertical scatter plots represents the ΔGP for one bacterium. Vertical bars represent the mean ± SEM of 47 or 49 bacteria from four independent experiments. The statistical significance of difference in the ΔGP between strains was determined using Mann-Whitney's test; \*\*p < 0.01.

a small and similar increase in GP in zone C1 with respect to the membrane for both strains (ΔGP above 0) (Figure 1B). However, further away from the bacteria in band C2 and C3, ΔGP remained positive for BCG WT, whereas GP returned to the membrane reference value for BCGΔ*mas* (Figure 1B). Hence, the mean GP increase was both higher and observed over a larger distance for the DIM-producing BCG WT strain than for the DIM-deficient BCGΔ*mas* mutant (Figure 1B). The membrane was therefore more apolar around BCG WT than around BCGΔ*mas*. Given the resolution of the microscope (around 250 nm), the observed GP increase in C1 might be caused by bacterial fluorescence extending into C1. Importantly, this

increase persisted up to zone C3 for BCG WT corresponding to 1–1.5 µm from the edge of the bacteria (Figure 1B). This distance is too big to be caused by an optical resolution effect. In *M. bovis* BCG, the deletion of *mas* induces not only DIM deficiency but also the loss of the structurally similar phenolic glycolipids (PGL). We therefore made a smaller-scale test with BCG mutants deficient for DIM or PGL only. BCGΔ*fad26* mutant with a spontaneous mutation in the *fad26* gene involved in DIM, but not PGL, biosynthesis (Simeone et al., 2010), displayed a lower mean ΔGP than the WT BCG strain (Figure S1c). In contrast, BCGΔ*pks15/1* harboring a mutation which blocks PGL synthesis without modifying DIM production (Constant et al., 2002),
<figure><img src="image_4.png" /></figure>

<figure><img src="image_5.png" /></figure>

<figure><img src="image_6.png" /></figure>

<figure><img src="image_7.png" /></figure>

**FIGURE 2** | DIM and PMA trigger the entry of the DIM-deficient H37Rv mutant and of zymosan into macrophages through a CR3-dependent process. (A) Macrophages were either left untreated (gray square) or treated at 37°C for 1 h with 70 µM DIM (red square) or the corresponding volume of chloroform:methanol (Ø vehicle control, gray square) or for 15 min with 50 nM PMA (green square) or the corresponding volume of DMSO (Ø vehicle control, gray square). Cells were then incubated for a further 30 min with either the non-relevant IgG1 or 10 µg/mL anti-CR3 blocking antibody 2LPM19c and put in contact for 1 h with zymosan at MOI 30:1. (B) Macrophages were successively incubated with 50 nM PMA (green symbol) or the corresponding volume of DMSO (Ø vehicle control, gray symbol) and with IgG1 or 10 µg/mL 2LPM19c and then exposed to GFP-expressing H37Rv (circle) or H37RvΔppsE (triangle) at MOI 10:1 for 1 h. (A,B) At the end of infection, cells were rinsed, fixed and processed for the quantification of infected macrophages using a Leica 43 DM-RB epifluorescence microscope. For each set of conditions, the experiments were performed in duplicate, and at least 100 cells were counted per slide. The percentage of cells having ingested at least one bacterium, or one particle, was determined. The values are mean ± SEM of 3–7 separate experiments. The significance of difference between control and treatment was evaluated using one-way ANOVA (A) or repeated measure ANOVA (B) followed by Bonferroni's multiple comparison test; \*p ≤ 0.05, \*\*p ≤ 0.01, \*\*\*p ≤ 0.001.

behaves similar to the WT BCG strain (Figure S1c). Together, our data indicate that DIM, and not PGL, contribute to the decrease in membrane polarity which extends around the site of bacterial contact over at least 1–1.5 µm. They also suggest that after transfer to the host membranes, DIM diffuse laterally in the membrane.

## DIM Induce the Activation of Complement Receptor 3 for an Optimal Invasion of Macrophages

We then asked whether this DIM-induced membrane perturbation could affect the phagocytic activity of CR3, the main receptor for Mtb entry in human macrophages. To address this question, we used zymosan, a yeast-derived polysaccharide particle, as a phagocytic prey for CR3 (Le Cabec et al., 2000). Macrophages were pre-incubated with exogenously added DIM or with a corresponding volume of chloroform:methanol dispersed in culture medium. We then evaluated the effect of the CR3-specific blocking antibody 2LPM19c on the

ability of macrophages to internalize zymosan (Figure 2A). In the absence of DIM, zymosan uptake was not significantly affected by adding 10µg/mL 2LPM19c, indicating that the CR3-dependent phagocytosis of zymosan is poorly efficient in resting macrophages (Figure 2A). Treatment of macrophages with DIM increased zymosan uptake by around 50% above the level without DIM (Figure 2A). We previously related this augmentation to the conical shape of DIM (Augenstreich et al., 2019). Here, we found that this DIM-induced increase was almost completely blocked by adding 2LPM19c (Figure 2A), supporting the proposal that DIM promote the engagement of CR3 in the phagocytosis of zymosan.

We then examined whether DIM affect surface expression of CR3. Using flow cytometry, we found no difference in the expression of CD11b subunit of CR3 between untreated macrophages and macrophages treated with DIM (Figure S2a). The functionality of CR3 also depends on the activation state of the receptor (Wright and Silverstein, 1982). We showed that treating the macrophages with 50 nM phorbol myristate acetate (PMA), a known agonist activating CR3 (Diamond and
Springer, 1993), increased phagocytosis of zymosan compared to control cells (Figure 2A). This effect is reduced by roughly 50% in the presence of 2LPM19c. The effect of PMA on phagocytosis therefore resembles that of purified DIM, supporting the hypothesis that DIM activate CR3 and facilitate zymosan phagocytosis.

Finally, we looked at what happens in the context of *Mtb*. Treatment of cells with 2LPM19c decreased by around 50% the internalization of H37Rv WT, confirming that *Mtb* uses mainly CR3 to invade human macrophages (Astarie-Dequeker et al., 2009). Interestingly, adding PMA did not improve the uptake of H37Rv WT, suggesting that CR3 is already activated by H37Rv (Figure 2B). In comparison, internalization of the DIM-deficient mutant H37RvΔ*ppsE*, which lacks a gene essential for DIM biosynthesis, is dramatically decreased and insensitive to CR3-blocking treatment, as previously described (Astarie-Dequeker et al., 2009). Adding PMA restored both the infectivity of the mutant and its inhibition by 2LPM19c to a level almost identical to that of the H37Rv WT strain (Figure 2B). Moreover, the CBRM1/5 antibody directed against the activation epitope of CR3 tended to block the uptake of both H37Rv in untreated and PMA-treated cells and of H37RvΔ*ppsE* in PMA-treated cells only (Figure S2b). Together, these data led us to conclude that DIM activate CR3, thereby ensuring an optimal invasion of macrophages.

## DIM Act in Concert With Mycobacterial Effectors to Promote Lysis of Biological Membranes

We next enquired how DIM contribute to the membrane-disrupting effect of *Mtb*. Using our assay based on calcein leakage from large unilamellar vesicles (LUV) and mass spectrometry, we corroborated that the lytic activity of the batch of rEsxA from BEI, which we previously attributed to EsxA (Augenstreich et al., 2017), was in fact due to residual detergent ASB14 (Figure S3a-d) (Conrad et al., 2017). These observations prompted us to test a native version of EsxA (nEsxA) purified from a culture of *Mtb* (De Jonge et al., 2007). As expected, we observed pH-dependent lytic activity of nEsxA on LUV made of POPC (Figure 3A) (De Jonge et al., 2007). The activity at pH 5 was significantly decreased after digestion by proteinase K, ruling out the presence of detergent (Figure 3A). Based on these positive results, we investigated whether DIM could modulate the activity of nEsxA. On liposomes of THP-1 lipids without DIM, the lytic activity of nEsxA at pH 5 was <10% (Figure 3B), well below that observed on POPC liposomes (Figure 3A). This difference can be explained by the higher membrane rigidity of THP-1 liposomes compared to POPC liposomes (Augenstreich et al., 2017; Ray et al., 2019). Nevertheless, incorporating DIM in THP-1 liposomes tended to potentiate by 35% the activity of nEsxA (Figure 3B). We also tested a recombinant version of EsxA (rEsxA) (Figure 3B) produced by us in *E. coli* and purified using the protocol provided by BEI Resources (Conrad et al., 2017) without the ASB-14-based endotoxin removal. We noticed that rESXA permeabilized liposomes more efficiently than nEsxA. We have no rational explanation for this difference

but we observed that DIM also increased the membranolytic activity of rEsxA (Figure 3B). This effect is specific to DIM, as the incorporation of the apolar triglyceride tripalmitin in THP-1 liposomes had no impact on the calcein leakage induced by rESXA (60 ± 30% and 53 ± 15%, *n* = 2, in the absence and the presence of 10% tripalmitin, respectively). We also observed that incorporation of DIM into THP-1-derived liposomes increased the lytic activity of the two pore-forming proteins, melittin and perforin-1 (Figure 3B). These observations indicated that DIM enhance the activity of different membranolytic compounds.

We also tested whether DIM work with EsxA in a mycobacterial context. To this end, we studied the hemolytic activity of several knock-out or knock-in mutants of *Mtb* and *M. bovis* BCG expressing combinations of DIM and EsxA. The H37Rv WT strain incubated for 48 h with human erythrocytes exhibited hemolytic activity (43+8%, *n* = 4). By comparison, the H37RvΔ*esxA* and H37RvΔ*mas* mutants were significantly less hemolytic (Figure 3C), indicating that both DIM and EsxA contribute to hemolysis. These results were confirmed in the BCG background, which has a partial deletion of ESX-1 and a functional inhibition of EsxA. Indeed, when BCG was genetically complemented with the ESX-1 region from *Mtb*, EsxA secretion was re-established (Augenstreich et al., 2017) and the resulting BCG::ESX-1 strain demonstrated higher hemolytic activity than the BCG WT strain and the DIM-deficient BCG::ESX-1Δ*mas* mutant (Figure 3C). Most notably, in both genetic backgrounds, the DIM-deficient strains exhibited significantly lower hemolytic activity than EsxA- or ESX-1-deficient strains, showing that DIM have an impact extending beyond EsxA/ESX-1.

## DISCUSSION

The notion of DIM as bacterial effectors subverting the host's immune responses has recently emerged (Rousseau et al., 2004; Astarie-Dequeker et al., 2009; Cambier et al., 2014; Passemar et al., 2014; Augenstreich et al., 2017; Barczak et al., 2017; Conrad et al., 2017; Quigley et al., 2017; Lerner et al., 2018), but the molecular mechanisms by which DIM exert their biological activity are poorly known. We have previously demonstrated that DIM are transferred from the envelope of *Mtb* to the membranes of host macrophages during infection and locally disturb their lipid organization (Astarie-Dequeker et al., 2009; Augenstreich et al., 2019). The next step was to identify the functional role of this transfer. We now establish that DIM induce a membrane perturbation, which spreads beyond the point of contact with the bacilli and improves the activity of proteins either embedded in host membranes, like CR3, or acting on these membranes, like EsxA and other membranolytic effectors.

Using C-laurdan combined with two-photon microscopy, we showed that the membrane polarity decreased at the site of interaction of BCG with a lipid membrane. This is consistent with our previous observation of a global decrease in membrane polarity in a population of macrophages infected by BCG (Astarie-Dequeker et al., 2009). As C-Laurdan allows identifying lipid domains with different packing degrees and lipid order (Owen et al., 2010), the observed decrease in
<figure><img src="image_8.png" /><figcaption>**FIGURE 3** | DIM potentiate EsxA membranolytic activity *in vitro*, and is required for EsxA hemolytic activity. (A) Left panel: the active form of the native EsxA (nEsxA) untreated (-) or digested with proteinase K (+) was analyzed by SDS-PAGE followed by Coomassie Blue staining; right panel: POPC liposomes were put in contact with 10 µM nEsxA at pH 5 or pH 7, or with 10 µM intact (C) or proteinase K-digested (PK) nEsxA at pH 5. (B) Calcein leakage in THP-1 liposomes supplemented or not with 10% of DIM (w/w) and incubated with 10 µM nEsxA (pH 5), rEsxA (pH 5), 50 nM melitin (pH 7), or 15 nM perforin-1 (PRF-1) (pH 7). Calcein was excited at 491 nm; its fluorescence was measured at 517 nm. The fluorescence intensity was converted in a percentage of leakage by the formula: % leakage = 100 × [($t$-t₀)/($t_{TX-100}$-t₀)] (Augenstreich et al., 2017). For each measurement, a new batch of liposome was made. Before treatment, we checked that the liposomes showed no calcein leakage (Augenstreich et al., 2017). The significance of difference in the percentage of leakage between untreated and membranolytic agent-treated liposomes was evaluated using the paired Student's t-test. (C) Strains of H37Rv or BCG producing a combination of EsxA and DIM were put in contact for 48 h at 37°C with 1 × 10⁷ erythrocytes at a MOI 50:1. Hemolysis was measured as described in Material and Methods and normalized to value of the corresponding DIM- and EsxA-proficient strain. The values are mean ± SEM of 4–5 separate experiments. The significance of difference between strains was determined using a one-way ANOVA test followed by Bonferroni's multiple comparison test; \*p ≤ 0.05, \*\*p ≤ 0.01, \*\*\*p ≤ 0.001; \*\*\*\*p ≤ 0.0001.</figcaption></figure>

polarity strongly supports the proposal that DIM locally disturb the lipid organization of host membranes and alter their physical properties. This can also be interpreted in the light of our recent findings showing that DIM adopt a conical molecular shape in a simple phospholipid bilayer and can disorganize such a bilayer by promoting the formation of non-bilayer (inverted hexagonal) membrane phases (Augenstreich et al., 2019). Remarkably, we observed that the local decrease

in membrane polarity extends over a distance of 1–1.5 µm around the bacterium. These observations provide experimental support to our molecular dynamic simulations predicting diffusion of DIM inside the membrane (see (Augenstreich et al., 2019); supplementary movie). Indeed, after transfer from the bacterium to the target membranes, DIM diffuse away from the contact site, causing a gradual fading of the polarity decrease.
Changes in membrane lipid composition and organization are generally thought to influence many cellular functions. Our findings go beyond the functional observations that *Mtb* uses envelope lipids to remodel the host membrane and divert macrophages functions (Laneelle and Tocanne, 1980; Sut et al., 1990; Welin et al., 2008; Augenstreich et al., 2019; Mishra et al., 2019). They provide evidence that insertion of lipids into host cell membranes helps the bacteria exploit membrane partners that direct the cell's responses. For instance, DIM increase CR3 activity for promoting phagocytosis. Like most integrins, CR3 is normally exposed at the cell surface in a low-activity state but can undergo changes that lead to increased activity, e.g., conformational changes leading to a state of higher affinity for their specific ligands (Liddington and Ginsberg, 2002). We found that DIM mimic at least in part the action of PMA, a known activator of CR3. For instance, DIM treatment increased the uptake of zymosan, which was almost completely abolished when CR3 was blocked by an anti-CR3 blocking antibody. Moreover, PMA restored the ability of a DIM-deficient mutant to infect macrophages equally efficiently as the WT strain, probably through the engagement of the active epitope of CR3 as assessed using CBRM1/5. All together, these data tend to indicate that DIM can also activate the CR3 receptor. Since CR3 can undergo activation yielding a conformational change that increases the affinity for its ligands, this activation would enable recognition by CR3 of PAMPs at the surface of *Mtb* leading to its uptake. Conical lipids like DIM may induce membrane curvature (Cooke and Deserno, 2006). The resulting membrane elastic stress may in turn modulate the function of integral membrane proteins (Phillips et al., 2009). It is therefore reasonable to hypothesize that DIM impose a curvature on the host's membrane that governs CR3's activation. This is consistent with the transmembrane nature of CR3 and its lateral mobility within cell membranes (Ross et al., 1992). DIM could also act on other membrane receptors cooperating with CR3 to carry out entry of mycobacteria, like CD14 which has been shown to induce an inside-out signaling pathway involving TLR2, leading to a CR3-dependent phagocytosis of *M. bovis* BCG (Sendide et al., 2005).

Our findings reinforce the proposal that DIM can work with *Mtb*EsxA (Augenstreich et al., 2017; Barczak et al., 2017). Using liposomes formed of THP-1 lipids, we demonstrate that DIM enhance the activity of *Mtb* EsxA. Combined with our previous data in macrophages, these results provide a reasonable explanation for DIM's involvement in the deterioration of the phagosomal membrane by *Mtb* (Augenstreich et al., 2017; Quigley et al., 2017). Interestingly, this potentiating effect of DIM is not specific to EsxA but also observed for the two pore-forming proteins melittin and perforin-1, and even for the detergent ASB-14 (Figure S3e). This strengthens the evidence that DIM act by perturbing their target membrane to promote the membrane-disruptive activities of these molecules. Furthermore, using gene-deleted *Mtb* mutants, we observed that the loss of DIM results in a lower hemolytic activity of *Mtb* than the loss of EsxA. As DIM

alone have no lytic activity, this implies that additional bacterial factors are involved in this process.

In conclusion, our findings reveal molecular mechanisms by which *Mtb* exploit DIM to rewire the host cells. Given their overall hydrophobic properties, DIM are able to reshape locally the host cell membrane in a way that targets cell signaling pathways and bacterial effectors, thus supporting their multifaceted role in the virulence of *Mtb*.

## DATA AVAILABILITY STATEMENT

All datasets presented in this study are included in the article/Supplementary Material.

## AUTHOR CONTRIBUTIONS

JA did part of the experiments, analyzed data, contributed to prepare figures, and reviewed the manuscript. EH contributed to experiments, critically analyzed the data, supervised part of the work, and reviewed the manuscript. FS, RS, and RB contributed selected rBCG strains and ESX-1 reagents and reviewed the manuscript. VG contributed to rESAT-6 purification. LM critically analyzed the data of rESAT-6 purification and reviewed the manuscript. SM critically analyzed the measurement of membrane polarity and help to prepare Figure 1. CC contributed to the construction of fluorescent recombinant mCherry BCG strains. CG supervised the work and reviewed the manuscript. CA-D did part of the experiments, supervised all the aspects of the study, including execution of the experiments and analysis, wrote the manuscript, and prepared figures. All authors contributed to the article and approved the submitted version.

## FUNDING

This work was supported by the European Union's Horizon 2020 Research and Innovation Program (TBVAC2020, 643381), the Agence Nationale de la Recherche (ANR-10-LABX-62-IBEID, ANR-14-JAMR-001-02, and ANR-16-CE15-0003), the Fondation pour la Recherche Médicale FRM (DEQ20130326471 and DEQ20160334879), and the Center National de la Recherche Scientifique (CNRS). JA was a recipient of a Ph.D. scholarship from the French government.

## ACKNOWLEDGMENTS

We thank the TRI-Genotoul Imaging facility (Toulouse, France). CA-D thanks T. Pennetier for technical assistance. We thank S. Dauvillier, E. Joly, and L. Salomé for helpful support and fruitful discussions.

## SUPPLEMENTARY MATERIAL

The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fcimb.2020.00420/full#supplementary-material
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**Conflict of Interest:** The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Copyright © 2020 Augenstreich, Haanappel, Sayes, Simeone, Guillet, Mazeres, Chalut, Mourey, Brosch, Guilhot and Astarie-Dequeker. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
## Supporting Information for:

### Phthiocerol dimycocerosates from *Mycobacterium tuberculosis* increase the membrane activity of bacterial effectors and host receptors

Jacques Augenstreich¹, Evert Haanappel¹, Fadel Sayes ², Roxane Simeone², Valérie Guillet¹, Serges Mazeres¹, Christian Chalut¹, Lionel Mourey¹, Roland Brosch², Christophe Guilhot¹*, C. Astarie-Dequeker¹*

* Correspondence should be addressed to C.A.-D. (email: catherine.astarie-dequeker@ipbs.fr), or to C.G. (email: christophe.guilhot@ipbs.fr)

This file includes:

- Figures and legends S1 to S3
<figure><img src="image_9.png" /></figure>

<figure><img src="image_10.png" /></figure>

<figure><img src="image_11.png" /></figure>

**Figure S1. Analysis of DIM-induced changes in membrane polarity of supported bilayers put in contact with BCG strains.** (a) Apolar lipids isolated from *M. bovis* BCG WT and the BCG *Δmas* mutant were resolved on thin layer chromatography in petroleum ether: diethyl ether (90:10 v/v) and compared to purified DIM. (b) *M. bovis* BCG::mCherry or BCGΔ*mas*.::mCherry were labelled with C-Laurdan, excited at 720 nm and the fluorescence spectrum of C-Laurdan was acquired for each strain in 18 channels ranging from 418 nm to 593 nm (channel width 9.7 nm), resulting in a stack of 18 images (λ-stack). (c) A POPC bilayer labelled with C-Laurdan was formed on a glass coverslip and incubated with 2 x 10⁶ bacteria for 20 min. The ΔGP was calculated as indicated in the legend of Figure 1. Each symbol in the vertical scatter plots represents the ΔGP of one bacterium. Histograms represent the mean ± SEM of 10 to 18 bacteria from 2 independent experiments. The statistical significance of difference in the ΔGP values between strains was determined using a Kruskal-Wallis' test followed by a Mann-Whitney's test, * p< 0.05.
<figure><img src="image_12.png" /></figure>

**Figure S2.** (a) DIM treatment does not affect CR3 expression at the surface of macrophages. Macrophages were incubated for 1 h at 37°C with 70 µM DIM (red line) or a corresponding volume of chloroform:methanol (vehicle control, blue line) and processed for evaluation of membrane-bound CR3 expression by flow cytometry. Briefly, cells were stained with anti-CD11b antibody-PE (BD Biosciences) (blue and red lines) or isotype control (black line). Flow cytometry was performed using a LSR-II flow cytometer analyzer (BD Bioscience) and the associated FACSDiva software. Data were analyzed using FlowJo software. (b) Blocking the activation epitope of CR3 with CBRM1/5 decreases the uptake of H37Rv in untreated and PMA-treated cells and the uptake of H37RvΔppsE in PMA-treated cells only. Macrophages were successively incubated for 15 min with 50 nM PMA or a corresponding volume of DMSO (vehicle control) followed by incubation for 30 min with either the non-relevant IgG1 or 10 µg/mL CBRM1/5 mouse antibody directed against the activation-specific epitope of CR3. Cells were then exposed for 1 h at 37°C to GFP-expressing H37Rv (white bar) or H37RvΔppsE (red bar) at MOI 10:1. The histogram represents the percentage of macrophages infected with H37Rv or H37RvΔppsE in treated and untreated macrophages, expressed with respect to H37Rv WT in untreated cells (set to 100%). The values are means ± SEM of 2 separate experiments.
<figure><img src="image_13.png" /><figcaption>**Figure S3.** The detergent ASB-14 is detected in rEsxA-BEI samples and exerts a membranolytic activity that can be modulated by DIM. (a) 10 µg of rEsxA from BEI (Reference standard, NR-14868) and melittin were digested by Proteinase K for 1h at 37°C. The digestion was verified by SDS-PAGE and Coomassie Blue staining. (b) The activity of intact or digested rEsxA-BEI (10 µM) and melittin (50 nM) was tested on POPC liposomes at pH7. (c) 2.5 µg of ASB-14 and 1 µg of rEsxA-BEI were analyzed by MALDI-TOF mass spectrometry. The mass spectrum shows three peaks representing ASB-14 ions at 435 Da (M+H⁺) and pseudomolecular ion (M+Na)⁺ and (M+K)⁺ at 457 Da and 473 Da respectively. (d) The membranolytic activity of ASB-14</figcaption></figure>
was tested at different concentrations on POPC liposomes using a calcein leakage
assay. (e) The membranolytic activity of 0.005% ASB-14 was tested on POPC
liposomes and THP-1 liposomes supplemented or not with 10% DIM (w/w). The
experiment was performed just once to show that the effect of DIM on the
membranolytic activity of our previous batch of rEsxA-BEI (Augenstreich et al., 2017)
was in fact an effect on the permeabilizing action of trace contaminations of ASB-14.