# Static magnetic fields modulate X-ray-induced DNA damage in human glioblastoma primary cells

Laura Teodori, Anna Giovanetti, Maria Cristina Albertini, Marco Rocchi, Barbara Perniconi, Maria Giovanna Valente, Dario Coletti

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Laura Teodori, Anna Giovanetti, Maria Cristina Albertini, Marco Rocchi, Barbara Perniconi, et al. Static magnetic fields modulate X-ray-induced DNA damage in human glioblastoma primary cells. Journal of Radiation Research, 2014, 55 (2), pp.218-227. 10.1093/jrr/rrt107. hal-01329395

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# Static magnetic fields modulate X-ray-induced DNA damage in human glioblastoma primary cells

Laura TEODORI<fnref n="1" />,<fnref n="2" />,*, Anna GIOVANETTI<fnref n="3" />, Maria Cristina ALBERTINI<fnref n="4" />, Marco ROCCHI<fnref n="5" />,
Barbara PERNICONI<fnref n="6" />, Maria Giovanna VALENTE<fnref n="2" /> and Dario COLETTI<fnref n="6" />

(Received 13 December 2012; revised 26 July 2013; accepted 2 August 2013)

Although static magnetic fields (SMFs) are used extensively in the occupational and medical fields, few comprehensive studies have investigated their possible genotoxic effect and the findings are controversial. With the advent of magnetic resonance imaging-guided radiation therapy, the potential effects of SMFs on ionizing radiation (IR) have become increasingly important. In this study we focused on the genotoxic effect of 80 mT SMFs, both alone and in combination with (i.e. preceding or following) X-ray (XR) irradiation, on primary glioblastoma cells in culture. The cells were exposed to: (i) SMFs alone; (ii) XRs alone; (iii) XR, with SMFs applied during recovery; (iv) SMFs both before and after XR irradiation. XR-induced DNA damage was analyzed by Single Cell Gel Electrophoresis assay (comet assay) using statistical tools designed to assess the tail DNA (TD) and tail length (TL) as indicators of DNA fragmentation. Mitochondrial membrane potential, known to be affected by IR, was assessed using the JC-1 mitochondrial probe. Our results showed that exposure of cells to 5 Gy of XR irradiation alone led to extensive DNA damage, which was significantly reduced by post-irradiation exposure to SMFs. The XR-induced loss of mitochondrial membrane potential was to a large extent averted by exposure to SMFs. These data suggest that SMFs modulate DNA damage and/or damage repair, possibly through a mechanism that affects mitochondria.

**Keywords:** static magnetic field; ionizing radiation; comet assay; DNA fragmentation; mitochondrial membrane potential; glioblastoma

## INTRODUCTION

Whether exposure to Static Magnetic Fields (SMFs) affects DNA integrity in human cells remains a matter of debate. Many studies suggest that SMFs have no effect on cell growth rate, with results showing that cell cycle distribution is not influenced even by extremely strong SMFs (up to a maximum of 7.05 T) [1]. By contrast, we (and others) show that SMFs do have structural and functional effects according to the cell types and to the intensity, duration and orientation of the field used [2-4]. The majority of evidence suggests that SMFs do not induce genotoxic effects [5, 6], however, some studies

have reported a mutagenic effect for SMFs [7, 8]. SMFs have been shown to reduce constitutive DNA damage signaling, which is considered to indicate DNA damage induced by endogenous oxidants, as has been demonstrated by analyzing oxidative phosphorylation [9]. It is of paramount importance to establish whether SMFs do cause DNA damage and, if so, to determine what doses, duration of exposure and type of SMFs (homogeneous or inhomogeneous) induce such damage, the underlying mechanisms of action, and which end-points need to be evaluated.

It has been suggested that free radicals play a key role in the mechanisms underlying DNA damage [10]. Indeed, one

<footnote n="1">¹Radiation Development and Application, UTAPRAD-DIM, ENEA, Via Enrico Fermi 45, Frascati, Rome 00044, Italy</footnote>

<footnote n="2">²Fondazione San Raffaele, SS Ceglie San Michele Km 1.2, Ceglie Messapica 72013, Italy</footnote>

<footnote n="3">³Radiation Biology and Human Health UTBIORAD, ENEA, Via Anguillarese 301, Casaccia, Rome 00123, Italy</footnote>

<footnote n="4">⁴Institute of Biochemistry, University of Urbino ‘Carlo Bo’, Via Saffi 2, Urbino 61029, Italy</footnote>

<footnote n="5">⁵Institute of Biomathematics, University of Urbino ‘Carlo Bo’, Via Saffi 2, Urbino 61029, Italy</footnote>

<footnote n="6">⁶UPMC Paris 06, UR4 Aging, Stress and Inflammation, 7 Quai Saint Bernard, Paris 75252, France</footnote>

<footnote>*Corresponding author. UTAPRAD-DIM, ENEA, via Enrico Fermi 45, 00044 Frascati (Rome), Italy.</footnote>

<footnote>Tel: +39-06-9400-5299; Fax: +39-06-94005770; Email: laura.teodori@enea.it</footnote>
of the potential mechanisms through which SMFs may inter-
act with the living organism is via electronic interactions, in
which magnetic fields influence the kinetics of the reactions
with radical pair intermediates [11]. It has also been postu-
lated that the magnetic field can increase the concentration of
free radicals in living cells [11, 12]. Free radicals have, in
turn, been shown to alter the mitochondrial membrane poten-
tial (ΔΨm) and to induce genetic damage [13, 14]. At the cel-
lular level, lipids, proteins, carbohydrates and nucleic acids
may be damaged by reactions with free radicals [15, 16].
These damaging reactions give rise to functional and morpho-
logical disturbances in the cell, due to oxidative/nitrosative/
carboxilative stress, which lead to reversible or irreversible
DNA damage [11]. The mechanisms underlying ionizing ra-
diation (IR)-induced DNA damage, unlike those associated
with exposure to SMFs, have been thoroughly investigated in
recent decades. The effects induced by IR, such as XRs, on
biological tissues, have been shown to be mediated by free
radicals. In recent years, IR has been used as a primary tool
for studying oxidative stress responses in various organisms
ranging from prokaryotes to higher eukaryotes. Molecular
insights into how reactive oxygen species (ROS) generated
by radiation can damage DNA have served as a basis for in-
vestigating the nature of mutagenesis, disease, aging, and in-
numerable biological processes ending in cell death [17, 18].
Mitochondria are assumed to be responsible for ROS produc-
tion [19]. Indeed, IR has been shown to upregulate the mito-
chondrial electron transport chain function [20]. ROS attack
DNA readily, generating a variety of DNA lesions, such as
oxidized bases and strand breaks [20–22]. It has also been
reported that magnetic fields interfere with the genotoxic ac-
tivity of physical and chemical agents [23, 24]. However,
there is a general lack of data regarding the effect of co-
exposure to SMFs and XRs. The aim of this study was to in-
vestigate the genotoxicity of exposure to SMFs alone, as well
as the genotoxicity of exposure to SMFs prior to and/or fol-
lowing XRs in a very sensitive primary line of human gliо-
blastoma cells obtained by surgical biopsy, which in a
previous study have been demonstrated to be highly respon-
sive to SMFs [2, 24].

MATERIALS AND METHODS

Cell culture

Cells were taken from a patient with Stage III multiform gliо-
blastoma undergoing a brain tumor resection for therapeutic
purposes. These cells were selected from one particular
patient out of four under study, since they showed the highest
sensitivity to chemical and physical agents [24]. The second-
ary use of the human specimens was approved by the hos-
pital ethical board, and the patients’ data were adequately
anonymized. Primary cultures were grown in RPMI medium
supplemented with 5% fetal calf serum and used in their
early passages. Cells in exponential growth, in triplicate Petri

dishes for each time-point under investigation, were exposed
to SMFs and/or XRs, as reported below and illustrated in the
scheme in Table 1. In order to submit all the samples to the
same environmental conditions (i.e. light and temperature),
the control cells underwent sham procedures (e.g. transporta-
tion to the XR generator, with a delay of return to the incuba-
tor equal to XR irradiation time) and are referred to as ‘sham’
throughout the paper. In the case of simple exposure to SMF,
sham-treated cells were submitted to the same environmental
conditions as SMF-treated cells, except for the lack of expos-
ure to SMFs.

SMF exposure

The exposure system has been previously described in detail
[3] and is briefly described here. SMFs of known flux dens-
ities were produced by custom-made 4 cm × 4 cm Neodym-
ium magnetic plaques. These magnetic plaques were placed
underneath the Petri dish at a 1-mm distance from the cell
monolayer in a custom-made device, which kept them sus-
pended in the incubator at least 10 cm from any metallic
surface (to avoid distortion of the field). Samples were also
placed > 10 cm from each other (at which distance the mag-
netic field of the plaque magnets is undetectable). The pos-
ition of the samples inside the incubator was randomly
chosen. The fields were axial, with the magnetic North (the
pole of the magnet which would be repulsed by the Earth’s
North magnetic pole) vector crossing the cells.

The magnitude of the magnetic field of each plaque was
80 ± 5 mT (1 mT = 10 G) and was checked by means of a
gaussmeter (Hall-effect Gaussmeter, GM04 Hirst Magnetic
Instruments, UK). The gaussmeter was also used to measure
the magnetic field inside the incubator (0.5 ± 0.02 mT),
which must be considered as background for all the cell cul-
tures used in this study, including sham cells, and added to
the 80 mT magnetic field for the exposed cells. Magnets, in
contrast with other exposure devices, do not produce tem-
perature variation. For schematic protocols of treatments and
sampling times see Table 1.

For the comet assay, experiments were repeated twice on
each of three independent culture dishes for each experimental

<table><caption>**Table 1.** Scheme of treatments, sampling times and assays performed</caption>
  <thead>
    <tr>
      <th>Treatment</th>
      <th>#</th>
      <th>° #</th>
      <th>°</th>
      <th>° #</th>
      <th>°</th>
    </tr>
  </thead>
  <tbody>
    <tr>
      <td>SHAM</td>
      <td>#</td>
      <td>° #</td>
      <td>°</td>
      <td>° #</td>
      <td>°</td>
    </tr>
    <tr>
      <td>XR</td>
      <td>#</td>
      <td>° #</td>
      <td></td>
      <td>° #</td>
      <td></td>
    </tr>
    <tr>
      <td>SMF</td>
      <td>#</td>
      <td>° #</td>
      <td>°</td>
      <td>° #</td>
      <td>°</td>
    </tr>
    <tr>
      <td>XR + SMF</td>
      <td>#</td>
      <td>° #</td>
      <td></td>
      <td>° #</td>
      <td></td>
    </tr>
    <tr>
      <td>SMF + XR + SMF</td>
      <td></td>
      <td></td>
      <td></td>
      <td>°</td>
      <td></td>
    </tr>
    <tr>
      <td>Sampling time (h)</td>
      <td>3</td>
      <td>6</td>
      <td>12</td>
      <td>20</td>
      <td>24</td>
    </tr>
  </tbody>
</table>

<footnote>° = comet assay, # = JC-1.</footnote>
point. For JC-1 analysis, experiments were repeated twice with a single measure within each experiment for each time-point.

## XR irradiation

Cells in triplicate Petri dishes for each time-point, were irradiated with 5 Gy XR generated by a CHF 320 G generator (Gilardoni, Mandello del Lario, LC, Italy) equipped with a Cu filter of 0.5 mm, operating at 250 keV, 15 mA, delivering a dose rate of 1.1 Gy/min. Cells underwent 6 or 20 h of recovery in the incubator before the comet assay. For ΔΨm investigation, cells were analyzed after 3, 6 and 20 h of recovery (Table 1).

## XR irradiation and SMF

Cell cultures were exposed to 5 Gy XRs, and during their recovery continuously exposed to SMF for 6 or 20 h. In addition, an experimental group was exposed to 6 h of SMF before XR irradiation, followed by recovery under SMF for 6 and 20 h (Table 1).

## Comet assay

The alkaline comet assay was performed on viable cells as previously described [25]. Dead and apoptotic cells were removed during rinsing. Trypan blue staining demonstrated that adherent cells contained no more than 2% and 4% of dead cells in sham cells and 5-Gy-irradiated cells, respectively. It has been reported that detaching cells with trypsin may increase the cells' ROS production [26]; however, scraping is considered worse; thus we used trypsin, so as to minimize cellular stress. Cells were thoroughly rinsed three times with 37°C, Ca- and Mg-free, sterile PBS, then incubated at 37°C with 1 ml 0.25% trypsin/EDTA solution for ~4 min, checking during this period the numbers of detached cells. Trypsinization was stopped by adding complete medium, and after this step cells were maintained on ice. Cells were gently resuspended, centrifuged at 200 G, then 20 µl of the cell pellet was mixed into 180 µl of 0.7% low-melting-point agarose in PBS (Ca- and Mg-free) at 38°C, and immediately pipetted onto a frosted glass microscope slide precoated with a layer of 1% normal-melting-point agarose, in PBS. Slides were covered with coverslips, set at 4°C for solidifying the agarose, then coverslips were removed and slides were incubated in a lysis solution (2.5 M NaCl, 10 mM Tris-HCl, 100 mM Na₂EDTA, NaOH to pH = 10, 1% Triton X-100, 10% dimethyl sulfoxide) for 45 min; after this step all the operations were performed at 4°C under dim light. After lysis, slides were rinsed for 10 min with electrophoresis buffer (1 mM Na₂EDTA, 300 mM NaOH, pH = 13) and placed for 20 min onto a horizontal electrophoresis unit containing the same electrophoresis buffer to allow DNA unwinding. Electrophoresis was conducted with the Sub-Cell GT System (15 × 25 cm) equipped with Power Pack 300 (Bio-Rad Laboratories Inc., Hercules, CA, USA) for 15 min (25 V, 300 mA). Subsequently, slides were gently washed in

neutralization buffer solution for 5 min (0.4 M Tris-HCl, pH = 7.5), dehydrated with an ethanol series (70, 85 and 100%), dried at room temperature and stored. For microscopy analysis, slides were stained with 2 mg/ml distilled water ethidium bromide. Where not differently indicated, all the chemicals were purchased from Sigma (St Louis, MO).

## Cell capture and analysis

As previously indicated we analyzed six Petri dishes for each experimental point (2 × 3 replicas). Randomly captured cells (150 cells) for each Petri dish (obtaining a total of 6 slides for each time-point) were examined at ×400 magnification using a fluorescent Axiolab Zeiss microscope (Carl Zeiss AG, Oberkochen, Germany) equipped with a Coolsnap cooled digital (CCD) camera (Roper Scientific, Princeton, NJ, USA). DNA migration was measured using the freely available CASP comet assay software program (http://www.casp.of.pl/). DNA migration was measured by analyzing the percentage of DNA in Tail (TD) and tail length (TL) [27].

## Data analysis

The comparison between sham and exposed samples (SMF and/or XR) was carried out by applying the analysis of variance (ANOVA) with Scheffé's multiple comparisons test. In all cases, *P*-values < 0.05 were considered statistically significant. The statistical tests were performed using MATLAB software.

## ΔΨm

Depolarization of ΔΨm was evaluated by the incorporation of 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethyl-benzimidazolcarbocyanin iodide (JC-1) (Molecular Probe, Invitrogen, Milan Italy). Cells, harvested as previously described, were stained at 3, 6 and 20 h of recovery after XR irradiation. Cells at the concentration of 10⁶/ml in PBS were incubated with 3 µg/ml (final concentration) JC-1 for 15 min in the dark at 37°C, from a stock solution of JC-1 at 1mg/ml in DMSO. Then, cells were washed once in PBS. Green vs red fluorescence was determined with a PASIII PARTEC flow cytometer (Partec, Muenster, Germany). At least 20 000 cells were counted for each cytogram. Estimation of emitted fluorescence was performed on forward/side scatter-gated cytograms.

# RESULTS

## SMF effects on glioblastoma cells

No significant difference was observed in either the TD or TL between sham-treated cells and cells exposed to 80 mT SMF (for either 6 or 12 h). By contrast, a significant difference emerged between the two groups of cells in both the TD and TL at 24 h exposure, as shown in Fig. 1. It is noteworthy that as the culture times grew longer, the sham cells also exhibited a significant increase in the comet parameters,
<figure><img src="image_2.png" /><figcaption>**Fig. 1.** Comet assay results of cells exposed to SMFs. The percentage of DNA in tail (TD) and 90% of tail length (TL) are shown in the upper and lower panel, respectively. The results of triplicate samples of two independent experiments are reported together with the SD for each time-point. Cells were exposed to an 80 ± 5 mT SMF in exponential growth for 6, 12 and 24 h. A statistically significant difference was observed (asterisk) between sham- and SMF-exposed cells after 24 h, and within both sham and exposed groups between 6 and 24 h of treatment (both TD and TL parameters) (*P* < 0.01).</figcaption></figure>

indicating that a certain amount of DNA fragmentation may
have occurred as a result of active metabolic endogenous ac-
tivity in these cells.

XR and SMF interactions on glioblastoma cells

As expected, the exposure of cells to 5 Gy XRs induced a
high degree of DNA fragmentation, as demonstrated by the
consistent increase in the TD and TL, at both 6 h and 20 h
following exposure (Figs 2 and 3), though it should be borne
in mind that the standard deviation was quite high (see

*Discussion*). The results for SMFs applied after XR irradi-
ation are also shown in Figs 2 and 3. They reveal a dramatic
reduction in DNA fragmentation when recovery was per-
formed under SMF exposure (*P* < 0.01). No major change
was observed when 6 h SMF pretreatment was performed
before XR irradiation.

ΔΨm

In order to assess the ΔΨm, known to be affected by XR ir-
radiation, we investigated the green vs red fluorescence JC-1
<figure><img src="image_3.png" /><figcaption>**Fig. 2.** Comet assay results of cells exposed to XR irradiation that underwent 6 h of recovery in the absence and in the presence of SMFs. TD and TL are shown in the upper and lower panels, respectively. The results of triplicate samples of two independent experiments are reported together with the SD. Five groups are reported in the figure. These groups are (i) sham: cells not exposed to any treatment but submitted to the same environmental conditions; (ii) XR: cells exposed to XR irradiation only; (iii) SMF: cells exposed to SMFs only; (iv) XR + SMF: cells exposed to SMFs during 6 h of recovery following XR irradiation; (v) SMF + XR + SMF: cells exposed to SMFs prior to XR irradiation and during 6 h of recovery. Recovery under SMFs significantly (asterisk) reduced the TD and TL (*P* < 0.001) compared with XR irradiation alone.</figcaption></figure>

staining using flow cytometry. The results demonstrated a consistent shift of the red fluorescence according to treatment. A representative histogram with the cumulative results of the four groups analyzed after 6 h of recovery is shown in Fig. 4. As expected, and as emerges in the figure, a dramatic decrease in 590 nm fluorescence, indicating ΔΨm, is observed in the XR samples (green line) when compared with the sham samples (blue line). A less marked shift in 590 nm fluorescence was observed in the SMF-treated cells.

What is very interesting is that the samples exposed to SMFs after XR irradiation unexpectedly displayed a significant recovery in the amount of 590 nm fluorescence, thereby pointing to rescue from ΔΨm, which is likely due to the presence of SMF during recovery.

The time-course of ΔΨm during recovery is illustrated in Fig. 5. These results highlight the ratios between the 590 nm fluorescence-positive cells and the 590 nm fluorescence-negative cells (i.e. RN2 vs RN1 of Fig. 5) analyzed after 3, 6
<figure><img src="image_4.png" /><figcaption>**Fig. 3.** Comet test results for cells exposed to 5 Gy XR irradiation that subsequently underwent 20 h of recovery with or without SMFs. TD and TL are shown in the upper and lower panels, respectively. The results of triplicate samples of two independent experiments are reported together with the SD. Five groups are reported in the figure. These groups are (i) sham: cells not exposed to any treatment but submitted to the same environmental conditions; (ii) XR: cells exposed to XR irradiation only; (iii) SMF: cells exposed to SMF only; (iv) XR + SMF: cells exposed to SMF during 20 h of recovery following XR irradiation; (v) SMF + XR + SMF: cells exposed to SMFs prior to XR irradiation and during 20 h of recovery. Recovery under SMFs significantly (asterisk) reduced the MDL and TL (*P* < 0.001) compared with XR irradiation alone.</figcaption></figure>

and 20 h of recovery following XR irradiation. The two-way
Anova test demonstrated an effect of both time (*P* < 0.001)
and treatment (*P* = 0.004), as well as an interaction effect (of
time and treatment) (*P* = 0.002). Using Scheffé's multiple
comparisons test, a significantly lower ΔΨm was observed at
3 h in the XR and XR + SMF groups, than in the sham cells.
However, the presence of SMFs for 3 h during recovery
yielded a significantly higher ΔΨm compared with XR ir-
radiation alone, thereby demonstrating the ability of SMFs to
modulate the ΔΨm. A significant difference was observed in

all the groups at 6 h, whereas at 20 h the ΔΨm was restored
and no statistical significance was observed between the
treatments. It is noteworthy, however, that both XR and
XR + SMF groups displayed a trend toward an increased
ΔΨm when compared with sham-treated cells.

DISCUSSION

Over the past decade, the possible biological effects (includ-
ing genotoxicity) of SMFs have been investigated in a wide
<figure><img src="image_5.png" /><figcaption>**Fig. 4.** Representative histogram of JC-1 590 nm fluorescence, indicative of the ΔΨm induced by the treatment, analyzed after 6 h of recovery following XR irradiation. Each fluorescence group is highlighted with a different color. A shift toward a reduced 590 fluorescence is evident in the XR-irradiated cells (green curve) compared with the sham cells (yellow curve). The presence of SMFs during the recovery phase following XR irradiation, instead, reduced the ΔΨm (blue curve).</figcaption></figure>

range of biological systems; for a comprehensive overview see Leszczynski [28] and the ICNIRP guidelines [29, 30]. Since the application of SMFs in industry, medicine and the environment (e.g. transportation) is growing, the possibility of occupational and general exposure is increasing, raising the need for more investigations on SMFs alone, as well as in combination with, other physical/chemical agents. SMFs are also considered to be a very promising tool for modulating cell orientation and differentiation in regenerative medicine and tissue engineering [3], and it is essential to be aware of any possible genotoxic or cytotoxic effects of SMFs. There only a few studies designed to investigate the interaction between non-IR and IR at the cellular level [31–33]. It should be noted also that, for some tumors, magnetic resonance imaging (MRI)-guided radiation therapy is showing promise when compared with traditional therapies [34], thus it is increasingly important to consider the potential influence of SMFs on IR.

Perusal of the literature reveals that the effects of SMFs vary according to the intensity of the magnetic force, the duration of the treatment and cellular systems [4, 35]. In particular, gene expression, including that of potentially oncogenic genes, seems to be modulated by SMFs [36]. Although the paucity and the heterogeneity of the studies on weak vs strong flux density SMFs makes it difficult to draw any clear-cut conclusion, the evidence suggests that both flux densities may have considerable biological outcomes. In our experimental procedure and in the cellular system used in the present study, SMFs were found to induce a time-dependent

increase in DNA fragmentation. This result is in accordance with other studies that have reported time- and dose-dependent genetic effects [35, 37].

Very surprisingly, SMFs modulated the XR response, reducing the degree of DNA fragmentation. It should be borne in mind that our findings were characterized by a high standard deviation, which may be ascribable to several factors: the intrinsic heterogeneity of the primary culture cells, reflecting the *in vivo* situation, might not contain a homogeneous cell genotype; differences in cell cycle phase sensitivity may also account for a range in the response. The difference that emerged from our experiments was, however, highly significant, despite dispersion of the data.

A recent study has also revealed a ‘beneficial’ effect of an homogeneous SMF on the repair of radiation-damaged DNA in leukocytes, though only after 4 h of recovery [38]. What explanation may be provided for our results showing that SMFs attenuate the level of DNA fragmentation and reduce the two comet parameters? It should be noted that SMFs prolongs the lifespan of reactive oxidants through the ‘radical pair mechanism’, which facilitates the interconversion of the singlet to the triplet excitation state (intersystem crossing) and would be expected to enhance rather than reduce DNA damage [10]. Yet another mechanism that might explain our findings is the interference by SMFs in the electron transport chain in mitochondria; cell respiration, which is the process that generates reactive oxidants, may be reduced by this interference. Surprisingly little information is available in the literature on the effect of SMFs on this process. If the process of electron transport, and consequently of oxidative phosphorylation, is perturbed by the SMF, oxidative DNA damage would be reduced. Our results demonstrate that a decreased mitochondrial depolarization occurs after XR irradiation in the presence of an SMF during recovery. Our previous observations on the same cells [24] demonstrated that the SMF rescues cells from apoptosis after they have been exposed to chemical agents. This result is in accordance with the attenuation of the loss of the ΔΨm induced by SMF exposure during XR irradiation recovery reported here. The present data shed light on the mechanism of interaction during combined treatment with IR and non-IR by pointing to a possible key role for mitochondria. The attenuation of the loss of the ΔΨm following exposure to an SMF as observed by us may impair the release of ROS from the mitochondrial interior, their direct transfer to nuclei, and also the induction of secondary radicals, thereby preventing oxidative DNA damage.

The fact that XR-induced DNA damage is still marked after 20 h of recovery, while the ΔΨm is completely restored is, despite not being the primary aim of this study, of considerable interest. A possible explanation for the persistent DNA damage might be the occurrence of the second wave of ROS, which occurs after XR irradiation [20, 39, 40]. Indeed, whereas IR instantaneously induces the formation of water
<figure><img src="image_6.png" /><figcaption>**Fig. 5.** Time-course of ΔΨm variation as determined by JC-1 staining. The figure shows the ratios between the positive and negative 590 nm fluorescent cells measured after 3, 6 and 20 h of recovery following XR irradiation, in the presence of SMFs (XR + SMF) or absence of SMFs (XR). The above ratios of cells exposed to SMFs alone (SMF) and of sham cells (SHAM) are also reported.</figcaption></figure>

radiolysis products that contain some ROS, the latter are also believed to be released secondarily by biological sources in irradiated cells. Mitochondria are assumed to be responsible for this secondarily IR-induced ROS production [20], though they may not be the only source. The type of ROS produced following radiation might vary depending on the time of recovery. In this regard, following XR irradiation, the results we obtained with 2'-7' dichlorofluorescin, which labels hydrogen peroxide, differed from those we obtained with dihydroethidium, which labels superoxide anions (data not shown), thus pointing to the existence of a highly complex network involving ROS, DNA damage-triggering, and the recovery time-scale. The uncoupled results we observed between DNA repair and ΔΨm 20 h after XR irradiation may also be due to the fact that DNA repair and recovery of ΔΨm follow different kinetic patterns.

The role of SMFs in protecting mitochondria, and consequently of modulating the genotoxic effects of IR, is clearly of considerable importance and warrants further investigation. The results of the present study represent a proof of principle that SMFs can interfere with IR and raise the very important question of whether the reduced XR-induced DNA damage caused by SMFs results in a reduced effectiveness of radiotherapy when guided by MRI. The magnetic field used in this study is much weaker than that used in MRI (which are in the order of magnitude of milliTesla and Tesla, respectively). However, remarkable biological effects have been shown for both weak and strong SMFs [24, 41, 42]. Notwithstanding, there is lack of exhaustive studies showing the intensity-dependence of these phenomena in the very

same experimental model, thus making it hard to extrapolate conclusions from weak to strong SMFs and vice versa. Since our study was limited to low intensity SMFs, the issue of comparing weak SMFs with strong SMFs, such as that used in MRI, warrants further investigation. Nonetheless, since the combined use of SMFs and IR is usually related to diagnostic applications, based on animal studies the antagonistic effects of this combination on biological structures might benefit patient health [43].

## FUNDING

This work was partially supported by the project of the Italian Minister for Health, MERIT from Fondazione San Raffaele, Project RBNE08HM7T.

## ACKNOWLEDGEMENTS

The authors are indebted to Prof. Zbygniew Darzinkiewicz for stimulating discussions and for reviewing the manuscript. The authors thank also Dr Dorota Halicka for her collaborative scientific support, Dr Matteo Manganelli and Dr Ing. Floreana Tramonti for their excellent technical help and Prof. Wolfgang Goehde for providing the flow cytometry instrument.

## REFERENCES

1. Schiffer IB, Schreiber WG, Graf R *et al*. No influence of magnetic fields on cell cycle progression using conditions relevant for patients during MRI. *Bioelectromagnetics* 2003;**24**:241–50.
2. Teodori L, Albertini MC, Uguccioni F *et al.* Static magnetic fields affect cell size, shape, orientation, and membrane surface of human glioblastoma cells, as demonstrated by electron, optic, and atomic force microscopy. *Cytometry A* 2006;**69**:75–85.

3. Coletti D, Teodori L, Albertini MC *et al.* Static magnetic fields enhance skeletal muscle differentiation *in vitro* by improving myoblast alignment. *Cytometry A* 2007;**71**:846–56.

4. Tenuzzo B, Chionna A, Panzarini E *et al.* Biological effects of 6 mT static magnetic fields: a comparative study in different cell types. *Bioelectromagnetics* 2006;**27**:560–77.

5. Chekhun VF, Lozovskaya YV, Lukyanova NY *et al.* Assessment of the cyto- and genotoxic effects of a nanoferromagnetic and a static magnetic field *in vivo*. *Cytol Genet* 2013;**47**:179–87.

6. McCann J, Dietrich F, Rafferty C *et al.* A critical review of the genotoxic potential of electric and magnetic fields. *Mutat Res* 1993;**297**:61–95.

7. Potenza L, Cucchiarini L, Piatti E *et al.* Effects of high static magnetic field exposure on different DNAs. *Bioelectromagnetics* 2004;**25**:352–5.

8. Takao K, Ikehata M, Nakagawa M. Estimation of genetic effects of a static magnetic field by a somatic cell test using mutagen-sensitive mutants of *Drosophila melanogaster*. *Bioelectrochemistry and Bioenergetics* 1995;**36**:95–100.

9. Halicka HD, Darzynkiewicz Z, Teodori L. Attenuation of constitutive ATM activation and H2AX phosphorylation in human leukemic TK6 cells by their exposure to static magnetic field. *Cell Cycle* 2009;**8**:3238–40.

10. Okano H. Effects of static magnetic fields in biology: role of free radicals. *Front Biosci* 2008;**13**:6106–25.

11. Jajte J, Grzegorczyk J, Zmyslony M *et al.* Effect of 7 mT static magnetic field and iron ions on rat lymphocytes: apoptosis, necrosis and free radical processes. *Bioelectrochemistry* 2002;**57**: 107–11.

12. Jouni FJ, Abdolmaleki P, Ghanati F. Oxidative stress in broad bean (*Vicia faba* L.) induced by static magnetic field under natural radioactivity. *Mutat Res* 2012;**741**:116–21.

13. Wang JQ, Chen Q, Wang X *et al.* Dysregulation of mitochondrial calcium signaling and superoxide flashes cause mitochondrial genomic DNA damage in Huntington disease. *J Biol Chem* 2013;**288**:3070–84.

14. Baker K, Staecker H. Low dose oxidative stress induces mitochondrial damage in hair cells. *Anat Rec (Hoboken)* 2012;**295**: 1868–76.

15. Duan J, Kasper DL. Oxidative depolymerization of polysaccharides by reactive oxygen/nitrogen species. *Glycobiology* 2011;**21**:401–9.

16. Brieger K, Schiavone S, Miller FJ, Jr *et al.* Reactive oxygen species: from health to disease. *Swiss Med Wkly* 2012;**142**: w13659.

17. Maynard S, Schurman SH, Harboe C *et al.* Base excision repair of oxidative DNA damage and association with cancer and aging. *Carcinogenesis* 2009;**30**:2–10.

18. Azzam EI, Jay-Gerin JP, Pain D. Ionizing radiation-induced metabolic oxidative stress and prolonged cell injury. *Cancer Lett* 2012;**327**:48–60.

19. Kikusato M, Toyomizu M. Crucial role of membrane potential in heat stress-induced overproduction of reactive oxygen

species in avian skeletal muscle mitochondria. *PLOS ONE* 2013;**8:e64412*.

20. Yamamori T, Yasui H, Yamazumi M *et al.* Ionizing radiation induces mitochondrial reactive oxygen species production accompanied by upregulation of mitochondrial electron transport chain function and mitochondrial content under control of the cell cycle checkpoint. *Free Radic Biol Med* 2012;**53**: 260–70.

21. Sedelnikova OA, Redon CE, Dickey JS *et al.* Role of oxidatively induced DNA lesions in human pathogenesis. *Mutat Res* 2010;**704**:152–9.

22. Wang Y. Bulky DNA lesions induced by reactive oxygen species. *Chem Res Toxicol* 2008;**21**:276–81.

23. Amara S, Abdelmelek H, Garrel C *et al.* Influence of static magnetic field on cadmium toxicity: study of oxidative stress and DNA damage in rat tissues. *J Trace Elem Med Biol* 2006;**20**:263–9.

24. Teodori L, Goehde W, Valente MG *et al.* Static magnetic fields affect calcium fluxes and inhibit stress-induced apoptosis in human glioblastoma cells. *Cytometry* 2002;**49**:143–9.

25. Giovanetti A, Deshpande T, Basso E. Persistence of genetic damage in mice exposed to low dose of X rays. *Int J Radiat Biol* 2008;**84**:227–35.

26. Dinnen RD, Mao Y, Fine RL. The use of fluorescent probes in the study of reactive oxygen species in pancreatic cancer cells. *Methods Mol Biol* 2013;**980**:321–9.

27. Collins AR. The comet assay for DNA damage and repair: principles, applications, and limitations. *Mol Biotechnol* 2004;**26**:249–61.

28. Leszczynski D. Rapporteur report: cellular, animal and epidemiological studies of the effects of static magnetic fields relevant to human health. *Prog Biophys Mol Biol* 2005;**87**:247–53.

29. Repacholi MH, Grandolfo M, Jammet H *et al.* Guidelines on limits of exposure to static magnetic fields. International Commission on Non-Ionizing Radiation Protection. *Health Phys* 1994;**66**:100–6.

30. Vecchia P, Matthes R, Feyching M *et al.* Amendment to the ICNIRP "Statement on medical magnetic resonance (MR) procedures; protection of patients". *Health Phys* 2009;**97**:259–61.

31. Nakahara T, Yaguchi H, Yoshida M *et al.* Effects of exposure of CHO-K1 cells to a 10-T static magnetic field. *Radiology* 2002;**224**:817–22.

32. Miyakoshi J. Effects of static magnetic fields at the cellular level. *Prog Biophys Mol Biol* 2005;**87**:213–23.

33. Sarvestani AS, Abdolmaleki P, Mowla SJ *et al.* Static magnetic fields aggravate the effects of ionizing radiation on cell cycle progression in bone marrow stem cells. *Micron* 2010;**41**: 101–4.

34. Nguyen PL, Chen MH, D'Amico AV *et al.* Magnetic resonance image-guided salvage brachytherapy after radiation in select men who initially presented with favorable-risk prostate cancer: a prospective phase 2 study. *Cancer* 2007;**110**:1485–92.

35. Suzuki Y, Ikehata M, Nakamura K *et al.* Induction of micronuclei in mice exposed to static magnetic fields. *Mutagenesis* 2001;**16**:499–501.

36. Potenza L, Ubaldi L, De Sanctis R *et al.* Effects of a static magnetic field on cell growth and gene expression in *Escherichia coli*. *Mutat Res* 2004;**561**:53–62.
37. Lee CH, Chen HM, Yeh LK *et al*. Dosage-dependent induction of behavioral decline in *Caenorhabditis elegans* by long-term treatment of static magnetic fields. *J Radiat Res* 2012;**53**: 24–32.

38. Kubinyi G, Zeitler Z, Thuroczy G *et al*. Effects of homogeneous and inhomogeneous static magnetic fields combined with gamma radiation on DNA and DNA repair. *Bioelectromagnetics* 2010;**31**:488–94.

39. Kaneyuki Y, Yoshino H, Kashiwakura I. Involvement of intra-cellular reactive oxygen species and mitochondria in the radio-sensitivity of human hematopoietic stem cells. *J Radiat Res* 2012;**53**:145–50.

40. Yoshino H, Kiminarita T, Matsushita Y *et al*. Response of the Nrf2 protection system in human monocytic cells after ionising irradiation. *Radiat Prot Dosimetry* 2012;**152**:104–8.

41. Teodori L, Grabarek J, Smolewski P *et al*. Exposure of cells to static magnetic field accelerates loss of integrity of plasma membrane during apoptosis. *Cytometry* 2002;**49**:113–8.

42. Sakurai T, Terashima S, Miyakoshi J. Effects of strong static magnetic fields used in magnetic resonance imaging on insulin-secreting cells. *Bioelectromagnetics* 2009;**30**:1–8.

43. Gu Y, Hasegawa T, Yamamoto Y *et al*. The combined effects of MRI and X-rays on ICR mouse embryos during organogenesis. *J Radiat Res* 2001;**42**:265–72.