# Determination of artemether and lumefantrine in anti-malarial fixed-dose combination tablets by microemulsion electrokinetic chromatography with short-end injection procedure

N'Cho Christophe Amin, Huguette Fabre, Marie-Dominique Blanchin, Jérôme Montels, Michèle Aké

► To cite this version:

N'Cho Christophe Amin, Huguette Fabre, Marie-Dominique Blanchin, Jérôme Montels, Michèle Aké. Determination of artemether and lumefantrine in anti-malarial fixed-dose combination tablets by microemulsion electrokinetic chromatography with short-end injection procedure. Malaria Journal, 2013, 12 (1), pp.202. 10.1186/1475-2875-12-202. hal-03564196

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<figure><img src="image_1.png" /></figure>
Determination of artemether and lumefantrine in
anti-malarial fixed-dose combination tablets by
microemulsion electrokinetic chromatography
with short-end injection procedure

N'Cho Christophe Amin¹, Huguette Fabre², Marie-Dominique Blanchin², Jérôme Montels² and Michèle Aké¹*

**Abstract**

**Background:** Artemether-lumefantrine (AL) combination therapy is now the most used anti-malarial treatment in the world. Quality control of AL formulations is still a major challenge in developing countries. Until now, only liquid chromatographic methods have been reported in the literature for their analysis. Capillary electrophoretic methods, which present various advantages (low price of capillary, low volumes of electrolyte consumption), may be an alternative to liquid chromatography methods. In this paper, a reliable method was developed and validated for the determination of AL in commercial fixed-dose combination tablets commercialized in Côte d'Ivoire.

**Methods:** Artemether and lumefantrine were determined by microemulsion electrokinetic chromatography using short-end injection procedure. The two analytes were extracted from tablets by acidified methanol. Pyrimethamine was used as internal standard. Separation was carried out in an uncoated fused silica capillary, 30 cm long × 50 µm internal diameter, using an effective length of 10 cm and a microemulsion composed of octane, butanol, sodium dodecyl sulfate and borate buffer as background electrolyte, a - 500 V.cm⁻¹ electric field and a detection wavelength of 214 nm.

**Results:** Artemether, lumefantrine and pyrimethamine were separated in 6 min. The method was reliable with respect to selectivity towards formulation excipients, linearity of the response function (r² > 0.998), recovery studies from synthetic tablets (in the range 99–101%), repeatability (relative standard deviation 1–3%, n = 7 analytical procedures). Application to four commercial formulations containing 20/120 mg of AL per tablet gave a content in good agreement with the declared content. However, the electropherogram of one tablet formulation showed the presence of an ingredient which was not declared.

**Conclusion:** The developed MEEKC method can be proposed as an alternative method to liquid chromatography for the determination of artemether and lumefantrine in fixed-dose combination tablet formulations.

**Keywords:** Antimalarial, Artemether, Lumefantrine, MEEKC, Short-end injection procedure

<footnote>* Correspondence: dominique_ake@yahoo.fr

¹Département de Chimie Analytique, Bromatologie, Chimie Minérale et Chimie Générale, UFR Sciences Pharmaceutiques et Biologiques - Université Félix Houphouët-Boigny, Abidjan BP V 34, Côte d'Ivoire

Full list of author information is available at the end of the article</footnote>
Background

Malaria is the most important infectious disease in the
world, with an estimated 274 million more cases and 1.1
million more deaths between 2001 and 2010 [1]. To im-
prove therapeutic efficacy and delay the development of
resistance, the World Health Organization has since
2001 recommended for antimalarial treatment, the use
of combination therapy based on the synergistic or addi-
tive potential of two or more drugs. Artemisinin-based
combination therapy, using artemether-lumefantrine
(AL) (Figure 1, [2,3]) and artesunate-amodiaquine (AS-
AQ), is currently considered as the first choice treatment
for *Plasmodium falciparum* malaria in endemic areas.
Tablets and capsules are used in fixed-dose combina-
tions (FDCs) which ensure that the two drugs are taken
together and in correct proportions.

The assay of the active substances in these formula-
tions is difficult due to the polarity difference between
the analytes and the absence of chromophore for artemi-
sinin derivatives (artesunate or artemether) present in
low proportion with respect to the associated anti-
malarial drug (weight ratios of 1/2.7 for AS/AQ and 1/6
for AL). For the assay of AS and AQ in FDCs, high per-
formance liquid chromatography (HPLC) [4-6] and capil-
lary electrophoresis (CE) methods have been proposed [7].
For AL, only HPLC methods [8-12] have been reported.
César *et al.* [8] were the first authors to propose a HPLC
method allowing a separation of the two analytes (within
5 min.) using a cyano stationary phase, an acetonitrile-
0.05% trifluoroacetic acidin water (60:40, v/v) mobile
phase and a 210 nm detection wavelength. Due to the high
lipophilicity of lumefantrine, a mixture of chloroform and
acetonitrile was used to ensure the complete extraction of
lumefantrine from tablets. Standard addition method was
used for artemether quantitation to compensate its low
absorbance in the extract. Two monographs were pub-
lished in 2009 and 2010 as United States Pharmacopoeia

SALMOUS standard for AL capsules [9] and in the Inter-
national Pharmacopoeia for AL tablets [10]. In both
monographs, an identical gradient ion-pairing method
with detection at two wavelengths (210 nm and 380 nm
for artemether and lumefantrine, respectively) is proposed.
Test solution (0.2 g L⁻¹ for artemether and 1.2 g L⁻¹ for
lumefantrine) is prepared by extracting the active sub-
stances with a complex diluent composed of ion-pair
reagent, water, propanol and acetonitrile. Under the
chromatographic conditions used, artemether and
lumefantrine are eluted at 19 min and 34 min. respectively
and the run time (RT) including the time for column re-
equilibration is about 55 min. In 2011, some authors [11],
stating that no HPLC method had been previously
reported, have developed an isocratic method providing
the separation in 12 min. using an ODS stationary phase,
a mobile phase composed of methanol-trifluoroacetic
acid- triethylamine buffer (80:20, v/v) adjusted to pH 2.8
and a wavelength detection of 210 nm. This paper is quite
confusing concerning the quantitation since a test solution
(0.8 g L⁻¹ for artemether and 4.8 g L⁻¹ in acidified metha-
nol) which is out of the calibration range and has a con-
centration different from the standard solution (0.2 g L⁻¹
artemether and 1 g L⁻¹ lumefantrine) is used. Very recently
Suleman *et al*. [12] have developed and validated a sta-
bility indicating assay for the simultaneous determin-
ation of AL tablets. After extraction by tetrahydrofuran,
artemether and lumefantrine are separated in isocratic
conditions using a fused-core amide stationary phase
and detection at 210 nm (β- artemether) and 335 nm
(lumefantrine).

A recent exhaustive literature survey on CE methods
applied to anti-malarials [13] has shown that up to date,
there are no CE methods reported for the assay of
artemether and/or lumefantrine as drug substances or in
drug formulations. Since CE presents the distinct ad-
vantage of reduced operating cost and low-cost CE

<figure><img src="image_2.png" /><figcaption>Figure 1 Chemical structure of artemether and lumefantrine with indication of pKa and log P values.</figcaption></figure>
instruments have been implemented in several develop-
ing countries [14,15] for the quality control of drugs and
detection of counterfeit or substandard formulations, the
possibility of using this technique for the assay of AL in
FDCs formulations is investigated in this paper.

**Methods**

Chemicals

De-ionized water doubly distilled was used throughout the study. All chemicals were of analytical grade. Pyrimethamine used as internal standard (IS), phosphoric acid, sodium dodecyl sulfate (SDS) and 1-butanol were purchased from Sigma Aldrich (Saint Quentin Fallavier, France). Lithium dodecylsulfate (LiDS) was from Fisher Scientific (New Jersey, United States of America), octane and di-sodium tetraborate decahydrate from Merck (Darmstadt, Germany). Artemether and lumefantrine were obtained from Quimdis (Levallois-Perret, France).

Pharmaceutical formulations, Artrine® (LIC Pharma,
Abidjan, Côte d'Ivoire), Coartem® (Novartis Pharm, Basel,
Switzerland), Cofantrine® (EGR Pharma, Maharashtra,
Inde) and Plasmocid® (Cipharm, Abidjan, Côte d'Ivoire)
were purchased in Côte d'Ivoire. All formulations have a
declared content of 20 mg of artemether and 120 mg of
lumefantrine per tablet.

Solutions

Background electrolyte solution

The microemulsion (ME) was prepared by accurately
weighing 0.81 g octane, 6.61 g 1-butanol, 3.31 g SDS and
89.27 g of 10 mM sodium tetraborate buffer in a 100 mL
flask. The mixture was sonicated for 20 min to form an
optically transparent ME which is stable for at least
three months.

Internal standard solution

The internal standard solution (ISS) was a 200 mg L⁻¹
pyrimethamine solution in methanol – water – phos-
phoric acid (93: 5: 2, v/v/v).

Mixed standard solution of artemether and lumefantrine

Approximately 20 mg lumefantrine and 200 mg artemether
accurately weighed, were transferred into a 20 mL glass
volumetric flask, sonicated and diluted to volume with ISS.
This solution diluted 1/10 (v/v) in the ME was the standard
solution (100 mg L⁻¹ lumefantrine, 1000 mg L⁻¹ artemether,
20 mg L⁻¹ IS).

Lumefantrine test solution

A portion of ten tablets finely powdered, equivalent to
about 10 mg lumefantrine, was transferred into a 10-mL
volumetric flask, sonicated for 20 min. and diluted to
volume with ISS. The supernatant obtained after centri-
fugation (10,000 rpm for 5 min.) and diluted 1/10 (v/v)

in the ME was the test solution for lumefantrine (theoret-
ical concentration 100 mg L⁻¹ lumefantrine, 20 mg L⁻¹ IS).

Artemether test solution

A portion of ten tablets finely powdered equivalent to about 10 mg artemether was transferred into a 10 mL volumetric flask, sonicated for 20 min. and diluted to volume with the ISS diluted 1/10 (v/v) in ME. The supernatant obtained after centrifugation (10,000 rpm for 5 min.) was the test solution for artemether (theoretical concentration 1000 mg L⁻¹ artemether, 20 mg L⁻¹ IS). Because of the physical instability of this microemulsion in the presence of methanol at a concentration higher than 8% v/v [16], standard and test solutions were analysed within six hours following their preparation.

**Apparatus and operating conditions**

All experiments were performed on a Beckman P/ACE
MDQ (Fullerton, CA) CE instrument equipped with a
DAD. All separations were carried out in an uncoated
fused-silica capillary, 30 cm long (10 cm to the detector),
50 µm inner diameter, 375 µm outside diameter
(Beckman), housed in a cartridge with a 200 × 800 µm
detector window. Prior to its first use, the capillary was
washed at 20 psi for 20 min. with a 0.1 M sodium hy-
droxide solution, and then flushed with water for 5 min.
On each working day, starting a sequence, the capillary
was rinsed at 20 psi for 5 min with 0.1 M sodium hy-
droxide, 5 min water and 5 min ME.

The optimal operating conditions were as follows: the
capillary was flushed with the background electrolyte
(BGE) for 2 min. using a rinse vial different from the
two separation vials. Sample introduction (~4 nL) was
performed on the detector side (short-end injection) by
hydrodynamic injection (0.2 psi for 5 s), followed by a
“wait” step in another vial containing the electrolyte to
prevent sample carryover on the outside capillary tip
and contamination of the separation electrolyte. Separ-
ation was carried out at 25°C, applying a - 15 kV voltage
(- 500 V.cm⁻¹) with the UV detector set at 214 nm. Sep-
aration vials were changed after 10 injections to take
into account buffer depletion.

Standard and test solutions were injected in duplicate.
Relative corrected peak areas (RCPA) corresponding to
areas/respective migration times (MTs) of analyte/IS
were used for calculations.

**Results and discussion**

Method development

The development of a CE method for AL was a rather
difficult task since artemether, which does not have
chromophore, is in low proportion (weight ratio 1/6 with
respect to lumefantrine) in the formulation. Furthermore,
lumefantrine is a highly hydrophobic compound (Figure 1).
Preliminary experiments were conducted using conventional long-end injection (effective length, 20 cm) and a 15 kV separation voltage (normal polarity) to keep the current developed below 100 µA.

### Selection of operation mode, electrolyte and separation conditions

The chemical structure (Figure 1) of artemether and lumefantrine shows that artemether is a non-ionizable compound, so that only micellar electrokinetic chromatography (MEKC) and MEEKC can be potential operation modes for a quantitative analysis of both compounds. In a previous paper, it was shown [7] that using a SDS-MEKC method (SDS 30 mM in 25 mM borate buffer pH 9.2, 500 V.cm⁻¹) lumefantrine was not eluted within 60 min. This was related to its insolubility in the background electrolyte. Addition of an organic modifier (methanol or acetonitrile) to the BGE at different concentrations up to 30% (v/v) to improve lumefantrine solubility could not achieve lumefantrine elution. Hence, further investigations were carried out in MEEKC, since in MEEKC the presence of a co-solvent (typically 1-butanol or 2-propanol) and an oil (typically octane or heptane) results in a great solubilizing power both for water-insoluble and water-soluble compounds [17]. MEEKC is particularly well suited for the analysis of very lipophilic compounds, such as fat-soluble vitamins or steroids [18,19] and has found a large use for the lipophilicity determination of chemical substances [13,20,21].

Several ME systems were investigated (Table 1), which gave a high resolution (> 5) and an acceptable current (< 90 µA) within an acceptable elution time. The ME composed of 0.81% w/w octane- 6.61% w/w 1-butanol- 3.31% w/w SDS- 89.27% w/w 10 mM borate buffer was selected in the further steps of development, as it gives the best compromise in terms of baseline, RT and operating cost by comparison to LiDS. With this ME, the analytes were eluted within 12 min. with an acceptable current and a resolution higher than 7, so that short-end injection technique (sample introduction at the detector side) could be used to reduce the RT. The robustness of this injection mode for quantitative analysis in

capillary zone electrophoresis [22-24], MEKC [25], and MEEKC [26] has been reported.

### Selection of an extraction diluent and injection solvent for artemether and lumefantrine

The choice of an injection solvent has a significant impact on separation efficiency in MEEKC as in MEKC, specially for lipophilic compounds [27]. Selection of an appropriate solvent to extract artemether and lumefantrine from tablets was a difficult challenge since they have very different polarities. In MEEKC, it is recommended whenever possible to dissolve the sample in the MEEKC buffer [28] to avoid baseline disturbance. However, lumefantrine is not soluble in the microemulsion but soluble in methanol acidified with phosphoric [11] or formic acid [29]. Because the use of an organic solvent results in a loss of efficiency as it disrupts the microemulsion environment adjacent to the injection zone, the methanolic extract was diluted (1/10, v/v), in the ME prior injection. In addition, because there is no stacking effect, low injection volumes were used to avoid peak broadening. Different injection volumes (from about 1.6 to 12 nL) were investigated. A volume of about 4 nL was found to give satisfactory peak shapes and reasonable signals.

### Selection of an internal standard

An IS was needed to take into account possible methanol evaporation during the extraction step, small variations in the injected volume. In addition, it takes into account variations due to temperature and electroosmotic flow (EOF) rate. Benzoic acid, sorbic acid, cinchonine and pyrimethamine were tested as IS. Pyrimethamine was selected as it gives a resolution > 2 with artemether and do not increase the analysis time.

### Capillary rinse between injections

Different capillary rinse procedures between injections were investigated. It was found that a short capillary rinse with the microemulsion was sufficient to obtain acceptable drift and repeatability of MTs (n = 6; relative standard deviation (RSD) < 1%). No fouling of the capillary was observed within 50 injections.

<table><caption>Table 1 Microemulsion systems tested</caption><thead><tr><td>Composition of the ME</td><td>Current developed, RT and migration order</td></tr></thead><tbody><tr><td>a) 0.81% w/w octane + 6.61% w/w 1-butanol + 3.31% w/w SDS + 89.27% w/w 10 mM borate buffer</td><td>+ 70 µA; RT around 12 min; MT<sub>artemether</sub> &lt; MT<sub>lumefantrine</sub></td></tr><tr><td>b) 0.81% w/w octane + 6.61% w/w 1-butanol + 3.31% w/w SDS + 89.27% w/w 20 mM borate buffer</td><td>+ 85 µA; RT around 15 min; MT<sub>artemether</sub> &lt; MT<sub>lumefantrine</sub></td></tr><tr><td>c) 0.81% w/w octane + 6.61% w/w 1-butanol + 3.31% w/w LiDS + 89.27% w/w 10 mM borate buffer</td><td>+ 60 µA; RT around 10 min; MT<sub>artemether</sub> &lt; MT<sub>lumefantrine</sub></td></tr><tr><td>d) 0.8% w/w octane + 6.6% w/w 1-butanol + 6.0% w/w SDS + 20% w/w isopropanol + 66.6% w/w 25 mM phosphate buffer</td><td>+67 µA; RT around 9 min; MT<sub>artemether</sub> &gt; MT<sub>lumefantrine</sub><br>baseline disturbance</td></tr></tbody></table>

Operating conditions: silica capillary, 30 cm (20 cm to the detector); injection 4 nL; separation voltage 15 kV (normal polarity); 25°C; λ = 214 nm.
### Selection of detection wavelength

Due to the lack of chromophore and the need of a sensitive detection for artemether, a 214 nm wavelength was selected for the determination of both analytes as it gives the best signal-to-noise ratio for artemether. Specimen electropherograms of a standard solution and test solutions recorded under the final operating conditions are given in Figure 2.

### Evaluation of method performances

The method was validated according to the international conference of harmonisation guideline [30].

### Selectivity

The non-interference of the ingredients present in the different commercial tablet formulations analysed (Table 2) was assessed by injecting placebo solutions of each formulation. No interference was noted at the MTs of the active substances and internal standard (Figure 2A).

### Linearity of the response function

The linearity of the response function (relative corrected peak area (RCPA) analyte/IS) vs analyte concentration was assessed by injecting in triplicate mixed standard solutions (in the range 600–1400 mg L⁻¹ for artemether and 60–140 mg L⁻¹ for lumefantrine) at five concentration levels corresponding to 60, 80, 100, 120 and 140% of the target concentration used in the assay. Corresponding regression equations were:

$$ \text{RCPA}(\text{artemether/IS}) = (0.00038 \pm 0.00001) \\ \text{artemether mg L}^{-1} \\ -(0.03 \pm 0.03); r^2 = 0.9998 $$

$$ \text{RCPA}(\text{lumefantrine/IS}) = (0.0346 \pm 0.0005) \\ \text{lumefantrine mg L}^{-1} \\ -(0.002 \pm 0.006); r^2 = 0.9987 $$

with the confidence intervals calculated at α = 0.05. Analysis of variance showed that the calibration graphs were linear and intersected the origin, showing that a single calibration solution may be used for routine analysis.

### Recovery studies

The accuracy of the method was assessed by performing recovery experiments on laboratory prepared formulations of Coartem®. Three sets of independent determinations were carried out on placebo powder spiked with known amounts of artemether and lumefantrine corresponding to 80, 100 and 120% of the target concentration.

<figure><img src="image_3.png" /></figure>

Recoveries of artemether and lumefantrine calculated against a standard solution at the target concentration prepared in duplicate are given in Table 3. Mean recoveries for artemether and lumefantrine are in the range 100-101% and 99-100% respectively.
<table><caption>**Table 2 Excipients of the commercial formulations analysed**</caption><thead><tr><th>Excipients</th><th>Artrine®</th><th>Coartem®</th><th>Cofantrine®</th><th>Plasmocid®</th></tr></thead><tbody><tr><td>Colloidal anhydrous silica</td><td></td><td>x</td><td></td><td></td></tr><tr><td>Hydroxypropylcellulose</td><td></td><td></td><td></td><td>x</td></tr><tr><td>Hypromellose</td><td></td><td>x</td><td></td><td>x</td></tr><tr><td>Isopropyl alcohol</td><td></td><td></td><td>x</td><td></td></tr><tr><td>Lactose</td><td></td><td></td><td>x</td><td></td></tr><tr><td>Magnesium stearate</td><td>x</td><td>x</td><td>x</td><td>x</td></tr><tr><td>Methylene chloride</td><td></td><td></td><td>x</td><td></td></tr><tr><td>Microcrystalline cellulose</td><td>x</td><td>x</td><td></td><td>x</td></tr><tr><td>Polysorbate 80 = tween 80</td><td></td><td>x</td><td>x</td><td></td></tr><tr><td>Sodium croscarmellose</td><td></td><td>x</td><td></td><td>x</td></tr><tr><td>Sodium laurylsulfate sodium</td><td></td><td></td><td></td><td>x</td></tr><tr><td>Starch</td><td>x</td><td></td><td>x</td><td></td></tr><tr><td>Talc</td><td></td><td></td><td></td><td>x</td></tr></tbody></table>

**System and procedure precision**

System precision was evaluated throughout the study. Similar MTs, relative MTs and RCPA were obtained in different circumstances (capillaries from different suppliers, analysis on different days, preparations of different microemulsions). In all cases, the RSD of corrected peak areas was better than 3% (n = 6 injections). These results confirm the robustness of this microemulsion previously reported in the literature [16].

The repeatability of the entire analytical procedure was evaluated by performing seven replicate determinations of artemether and lumefrantrine in commercial Coartem® tablets. The repeatability expressed as the RSD was 1.8% for artemether and 3% for lumefrantrine.

**Limit of detection and quantitation**

The limits of detection (signal-to-noise ratio of 3) and quantification (signal-to-noise ratio of 10) were evaluated from standard solutions of artemether and lumefrantrine. Limits of detection were about 164 mg L⁻¹ for artemether and 3 mg L⁻¹ for lumefrantrine, which correspond to 4 mg of artemether and 3.5 mg of lumefrantrine per tablet.

Limits of quantitation were 548 mg L⁻¹ for artemether and 6 mg L⁻¹ for lumefrantrine, which correspond to 13.5 mg of artemether and 12 mg of lumefrantrine per tablet.

<table><caption>**Table 3 Recovery data for artemether and lumefrantrine from laboratory prepared formulations of Coartem®**</caption><thead><tr><th>Exp. No.</th><th>% of the target concentration</th><th>Added amount (mg)</th><th>Found amount (mg)</th><th>Recovery (%)</th><th>Mean recovery ± RSD (%)</th></tr></thead><tbody><tr><td colspan="6"><strong>Artemether</strong></td></tr><tr><td>1</td><td></td><td>7.60</td><td>7.73</td><td>101.7</td><td></td></tr><tr><td>2</td><td>80</td><td>7.87</td><td>7.65</td><td>97.2</td><td>100.8 ± 3.2</td></tr><tr><td>3</td><td></td><td>8.21</td><td>8.48</td><td>103.3</td><td></td></tr><tr><td>1</td><td></td><td>9.97</td><td>9.78</td><td>98.1</td><td></td></tr><tr><td>2</td><td>100</td><td>10.23</td><td>10.75</td><td>104.2</td><td>100.5 ± 3.3</td></tr><tr><td>3</td><td></td><td>10.52</td><td>10.42</td><td>99.0</td><td></td></tr><tr><td>1</td><td></td><td>11.86</td><td>11.91</td><td>100.4</td><td></td></tr><tr><td>2</td><td>120</td><td>12.25</td><td>12.43</td><td>101.5</td><td>100.3 ± 1.2</td></tr><tr><td>3</td><td></td><td>12.72</td><td>12.59</td><td>99.0</td><td></td></tr><tr><td colspan="6"><strong>Lumefantrine</strong></td></tr><tr><td>1</td><td></td><td>7.55</td><td>7.64</td><td>101.2</td><td></td></tr><tr><td>2</td><td>80</td><td>7.77</td><td>7.77</td><td>100.0</td><td>99.8 ± 1.4</td></tr><tr><td>3</td><td></td><td>7.85</td><td>7.72</td><td>98.3</td><td></td></tr><tr><td>1</td><td></td><td>10.16</td><td>10.28</td><td>101.2</td><td></td></tr><tr><td>2</td><td>100</td><td>10.19</td><td>10.01</td><td>98.2</td><td>99.3 ± 1.7</td></tr><tr><td>3</td><td></td><td>10.73</td><td>10.55</td><td>98.3</td><td></td></tr><tr><td>1</td><td></td><td>11.75</td><td>11.48</td><td>97.7</td><td></td></tr><tr><td>2</td><td>120</td><td>11.93</td><td>11.85</td><td>99.3</td><td>98.8 ± 0.9</td></tr><tr><td>3</td><td></td><td>12.38</td><td>12.30</td><td>99.4</td><td></td></tr></tbody></table>
Analysis of different tablet formulations

Four commercial tablet formulations were analyzed for active substance content with the proposed method. Duplicate determinations were carried out except for Coartem® (seven determinations). The respective amounts of artemether/lumefantrine were 102.1/98.6, 101.4/98.1, 99.2/96.0 and 96.7/101.2 percent of the declared content for Artrine®, Coartem®, Cofantrine® and Plasmocid® tablets. The formulations comply with the requirements (90–110% of the label claim) of the International Pharmacopeia. The corresponding electropherograms are presented in Figure 2B and 2C. An unknown peak is present in the electropherogram of Cofantrine® tablets which does not correspond to a declared excipient. Its presence was confirmed in another batch of Cofantrine® tablet.

Conclusion

The aim of this study was to test if CE could be used as
an alternative technique to liquid chromatography for
the assay of fixed-dose combination tablets of AL. We
have shown that MEEKC is well suited for this purpose.
Satisfactory results were obtained for method validation
with respect to selectivity, linearity of the response func-
tion, recovery experiments and precision. In comparison
with the HPLC methods reported in the literature, the
disadvantage of MEEKC method developed is that the
quantitation of the active substances requires the prepar-
ation of two test solutions which increases the analysis
time. However, this method presents several advantages.
The total volume of organic solvent used for analyte ex-
traction is dramatically lower (about five times) than that
used in LC. Concerning the separation step, CE present
the distinct advantage of reduced operating cost in terms
of elution solvent (a few milliliters per day) and capillary
cost (about 4 US dollars for a 30 cm silica capillary). In
contrast, HPLC uses large volumes of mobile phase for
column equilibration and elution and expensive chroma-
tographic columns. Hence, in developing countries where
financial resources are very limited, CE could be the tech-
nique of choice.

Abbreviations

AL: Artemether-lumefantrine; AQ: Amodiaquine; AS: Artesunate;
BGE: Background electrolyte; CE: Capillary electrophoresis;
EOF: Electroosmotic flow; FDC: Fixed-dose combination; HPLC: High
performance liquid chromatography; IS: Internal standard; ISS: Internal
standard solution; LiDS: Lithium dodecyl sulfate; ME: Microemulsion;
MEKC: Micellar electrokinetic chromatography; MEEKC: Microemulsion
electrokinetic chromatography; MT: Migration time; RPCA: Relative corrected
peak area; RT: Run time; SDS: Sodium dodecyl sulfate.

Competing interests

The authors declare that they have no competing interests.

Authors’ contributions

NCA study design, sample preparation, data collection, analysis and
interpretation of data, drafting of manuscript. HF conception of the study,

supervision on the progress of the study and revision of the manuscript.
M-DB study design and manuscript preparation. JM technical contribution.
MA study design, supervision and manuscript preparation. All authors read
and approved the final manuscript.

Author details

¹Département de Chimie Analytique, Bromatologie, Chimie Minérale et Chimie Générale, UFR Sciences Pharmaceutiques et Biologiques - Université Félix Houphouët-Boigny, Abidjan BP V 34, Côte d'Ivoire. ²Laboratoire de Chimie Analytique, Contrôle physico-chimique des médicaments, Institut des Biomolécules Max Mousseron, UMR 5247, UFR Pharmacie, Université Montpellier 1, Montpellier BP 14491-34093, France.

Received: 27 March 2013 Accepted: 31 May 2013

Published: 13 June 2013

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doi:10.1186/1475-2875-12-202

**Cite this article as:** Amin et al.: Determination of artemether and lumefantrine in anti-malarial fixed-dose combination tablets by microemulsion electrokinetic chromatography with short-end injection procedure. *Malaria Journal* 2013 **12**:202.

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