# The diversity of molecular mechanisms of carbonate biomineralization by bacteria

Sigrid Görgen, Karim Benzerara, Fériel Skouri-Panet, Muriel Gugger, Franck

Chauvat, Corinne Cassier-Chauvat

► To cite this version:

Sigrid Görgen, Karim Benzerara, Fériel Skouri-Panet, Muriel Gugger, Franck Chauvat, et al. The diversity of molecular mechanisms of carbonate biomineralization by bacteria. Discover Materials, 2021, 1, pp.2. 10.1007/s43939-020-00001-9. hal-03037108

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Review

# The diversity of molecular mechanisms of carbonate biomineralization by bacteria

Sigrid Görgen¹,³ · Karim Benzerara¹ · Fériel Skouri-Panet¹ · Muriel Gugger² · Franck Chauvat³ · Corinne Cassier-Chauvat³

Received: 1 June 2020 / Accepted: 23 October 2020

© The Author(s) 2020 OPEN

## Abstract

Although biomineralization of CaCO₃ is widespread in Bacteria and Archaea, the molecular mechanisms involved in this process remain less known than those used by Eukaryotes. A better understanding of these mechanisms is crucial for a broad diversity of studies including those (i) aiming at assessing the role of bacteria in the geochemical cycles of Ca and C, (ii) investigating the process of fossilization, and (iii) engineering applications using bacterially mediated CaCO₃ mineralization. Different types of bacterially-mediated mineralization modes have been distinguished depending on whether they are influenced (by extracellular organic molecules), induced (by metabolic activity) or controlled (by specific genes). In the first two types, mineralization is usually extracellular, while it is intracellular for the two ascertained cases of controlled bacterial mineralization. In this review, we list a large number of cases illustrating the three different modes of bacterially-mediated CaCO₃ mineralization. Overall, this shows the broad diversity of metabolic pathways, organic molecules and thereby microorganisms that can biomineralize CaCO₃. Providing an improved understanding of the mechanisms involved and a good knowledge of the molecular drivers of carbonatogenesis, the increasing number of (meta)-omics studies may help in the future to estimate the significance of bacterially mediated CaCO₃ mineralization.

**Keywords** Prokaryote · Biologically-influenced mineralization · Biologically-induced mineralization · Biologically-controlled mineralization · Alkalinity engine · MICP · Heterogeneous nucleation · ACC · Exopolymeric substances

## Abbreviations

ACC Amorphous calcium carbonate

CA Carbonic anhydrase

CEMOVIS Cryo-electron microscopy of vitreous section

EPS Extracellular polymeric substances

iACC Intracellular amorphous Ca-carbonate

**Electronic supplementary material** The online version of this article (https://doi.org/10.1007/s43939-020-00001-9) contains supplementary material, which is available to authorized users.

✉ Karim Benzerara, karim.benzerara@sorbonne-universite.fr; Sigrid Görgen, sigrid.gorgen@sorbonne-universite.fr; Fériel Skouri-Panet, feriel.skouri-panet@sorbonne-universite.fr; Muriel Gugger, muriel.gugger@pasteur.fr; Franck Chauvat, Franck.CHAUVAT@cea.fr; Corinne Cassier-Chauvat, Corinne.CASSIER-CHAUVAT@cea.fr | ¹Institut de Minéralogie, de Physique Des Matériaux Et de Cosmochimie, Sorbonne Université, UMR CNRS 7590, Muséum National D'Histoire Naturelle, Paris, France. ²Collection Des Cyanobactéries, Institut Pasteur, 75724 Paris Cedex 15, France. ³Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), 91198 Gif-sur-Yvette, France.

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# 1 Background

Here, we define biomineralization in the broadest sense as the process by which organisms form minerals, i.e. including both indirect and controlled formation pathways. Although biomineralization has been extensively studied in eukaryotes, it is also widespread among Bacteria and Archaea [1, 2].

Bacterial biomineralization has been known for more than a century. Ehrenberg discovered the iron-oxidizer *Gallionella ferruginea* in 1838, which biomineralizes iron oxyhydroxides on extracellular stalks [3]. Schewiakoff in 1893 described intracellular biomineralization by *Achromatium oxaliferum*, although at that time the taxonomic affiliation of this giant sulfur bacterium was uncertain and the identity of the biominerals misidentified [4]. In 1903, Nadson showed that calcium carbonate deposits in Lake Veisowe, Russia, could have originated from bacterial activity [5]. Later on, Drew in 1911 suggested that marine denitrifying bacteria played an active role in the precipitation of calcium carbonates with a potential geological significance of this process [6]. Kalkowsky in 1908 coined the word stromatolite (Stromatolith) insisting on the importance of microorganisms in the formation of these carbonate rocks [7], which are now known to be populated by a high diversity of microbes [8].

More recently, studies have strengthened the idea that bacterial biomineralization is pervasive and very diverse. Boquet in 1973 showed that diverse soil bacteria produce calcium carbonates [9]. A broad diversity of carbonate mineral phases can be formed by bacteria including the different polymorphs of Ca-carbonates: calcite but also, aragonite [10] and vaterite [11], hydrated Ca-carbonates such as monohydrocalcite [12, 13], amorphous (Ca, Sr, Ba, Ra)-carbonates [14, 15], dolomite (Ca-Mg-CO₃) [16], hydromagnesite (Mg₅(CO₃)₄(OH)₂·4H₂O) [17], or siderite (FeCO₃) [18].

Diverse objectives have motivated studies about bacterial carbonate biomineralization (carbonatogenesis): (1) some studies aimed at quantifying the impact of bacteria on modern and ancient geochemical cycles of C and/or Ca [19, 20]. It has been convincingly argued that bacterial biomineralization has massively contributed to the formation of large-scale carbonate deposits all over Earth's history [20, 21]. (2) Similarly, it has been suggested that bacterial biomineralization can favor fossilization and the preservation of traces of life in ancient rocks [22]. Consistently, bacterial carbonate biominerals are prone targets for searching traces of ancient life on Earth or Mars [23, 24]. For example, carbonate microbialites, which include stromatolites, are emblematic byproducts of bacterial biomineralization, considered as among the oldest traces of life [25–27]. (3) In addition, an active field of research aims at using bacterial carbonate biomineralization for diverse engineering applications. For example, the removal of polluting heavy metals or radionuclides (e.g. Ra, Sr) may be achieved by their co-precipitation with calcium carbonate by biomineralizing bacteria [15, 28]. Alternatively, bacterial carbonate biomineralization has been proposed as a strategy for carbon capture and storage [29]. Microbially enhanced oil recovery by plugging highly permeable rocks with bacterial carbonate biominerals has also been considered [30, 31]. Finally, bacterial carbonate biomineralization can be used for stabilizing soils and sediments [32], and restoring weathered limestone and cement monuments [30, 33]. More details on the use of microbially precipitated CaCO₃ as biomaterials can be found in several recent review papers [34–38].

For all these basic and applied science applications, there are crucial needs to understand the mechanisms involved in the mediation of carbonate precipitation by bacteria. Mineral precipitation can be triggered by: (1) the increase of the saturation index of the solution surrounding cells with this mineral phase. In the case of carbonate mineral phases, the saturation index increases with calcium ion activity (Ca²⁺) and carbonate ion activity (CO₃²⁻), hence bicarbonate ion activity (HCO₃⁻) and pH; (2) the decrease of the nucleation energy barrier. In that regard, some molecules produced by the cells act as preferential nucleation sites and favor heterogeneous nucleation [39, 40]. Following these principles, different biomineralization pathways have been distinguished [41]. (a) Biologically influenced mineralization comprises cases when mineral precipitation is favored by nucleation on bacterially produced organic polymers, such as those composing cell surfaces or extracellular polymeric substances (EPS). In such cases, biomineralization occurs in already saturated solution and does not require the cells to be metabolically active. (b) Biologically induced mineralization encompasses all cases when the precipitation of carbonate mineral phases results from chemical modifications of the microenvironment surrounding cells induced by their metabolic activities. These chemical shifts mostly include an increase of pH, or [HCO₃⁻] at constant pH. This is termed the “alkalinity engine” [41]. (c) Biologically controlled mineralization groups cases when a specific, genetic control of some of the mineral precipitation steps (e.g. nucleation and/or growth) occurs. As detailed below, there are known cases of bacterial carbonatogenesis for all three classes. Alternatively, biomineralization pathways can be discriminated as active vs passive based on whether they cost energy (they depend on metabolically active cells) or not (they need cells not necessarily alive). Lastly, biomineralization processes can be distinguished based on their

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localization respectively to the cell (intra- or extra-cellular). While bacterial carbonatogenesis has often been considered as extracellular, numerous environmentally significant bacteria perform intracellular biomimicry [42, 43].

Overall, to infer whether carbonatogenesis depends on specific genes (controlled mineralization), it is important to decipher the operating molecular mechanisms, and identify the templating molecules or the metabolic pathways involved. This will be useful to better interpret the massive data generated by the increasing use of omics approaches such as metagenomics, metatranscriptomics, metaproteomics to study biomimicry in microbial ecosystems, such as carbonate microbialites [8, 44] or calcifying soil microbes [45]. These approaches may provide information about the carbonatogenesis potential of an ecosystem provided if molecular mechanisms favoring or inhibiting biomimicry are sufficiently understood. Here, we review some of our current knowledge about the diversity of bacterial carbonate biomimicry processes and the underlying molecular mechanisms (Fig. 1). We also highlight gaps that will need to be filled to better use omics data in the future. Finally, we shed light on intracellular carbonate biomimicry that is often overlooked although current studies suggest that it is more widespread than previously thought.

<figure><img src="image_4.png" /><figcaption>**Fig. 1** Classification of the CaCO₃ biomimicry processes found in bacteria: a biologically influenced mineralization; b biologically induced mineralization; c biologically controlled mineralization. The grey hexagons represent CaCO₃ precipitates. Ca²⁺ cations can be replaced by, e.g. other alkaline earth elements such as Mg²⁺, Sr²⁺, Ba²⁺</figcaption></figure>
# 2 Main text

## 2.1 Bacterially influenced mineralization of calcium carbonates

This mode of mineralization has been sometimes named organomineralization by Trichet and Défarge [46]. A broad diversity of molecules, mostly (glyco-)proteins and polysaccharides, has been shown to impact the nucleation of carbonates in eukaryotes and metazoans [47]. However, they are less documented in bacteria. There are distinct supporting theories behind that: first, it has been suggested that a continuum exists between biomineralization and cation adsorption by diverse functional groups at the bacterial surfaces/exopolysaccharides [48]. In this respect, the local adsorption of cations such as Ca²⁺ or Mg²⁺ may drive the subsequent nucleation. Alternatively, it has been convincingly shown that diverse organic molecules, including polysaccharides, favor nucleation by decreasing the net interfacial energy between the growing crystal, the substrate and the solution [40] (see Additional file 1).

### 2.1.1 Influence of extracellular polymeric substances (EPS)

EPS are a complex mixture of high-molecular-weight polymers, mostly composed of polysaccharides with some nucleic acids, lipids, proteins, and other polymers such as humic acids [49]. EPS are a major component of biofilms, sometimes accounting for more than 90% of their dry mass [49], and support the aggregation of cells and attachment to surfaces [50]. Polysaccharides have been repeatedly observed as intimately and pervasively associated with carbonate mineral grains suggesting their involvement in mineral precipitation, possibly through mineral templating [51, 52] (Fig. 2). The

<figure><img src="image_5.png" /><figcaption>**Fig. 2** Some diversity of the molecules involved in biologically influenced mineralization of CaCO₃: **a** extracellular polymeric substances, comprising polysaccharides and proteins; **b** surface layers; **c** cell walls comprising teichoic and/or dipicolinic acid functions and/or proteoglycans and glycoproteins</figcaption></figure>

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diversity of bacteria capable of producing EPS is tremendous, including Cyanobacteria, sulfate-reducing bacteria and other diverse Proteobacteria [53]. By studying functional genes in lacustrine carbonate microbialites, Saghaï et al. [8] showed that genes associated with trehalose and rhamnose synthesis were mostly affiliated to Cyanobacteria, Alphapro-teobacteria and Gammaproteobacteria. Genes involved in the metabolism of other sugars were dominated by some of these phyla and additional ones. Bacteria produce hundreds of different EPS with various chemical structures [54]. This chemical and structural diversity of EPS relies on the identity and number of the saccharide monomers, the glycosidic linkages and the nature of functional groups. There is also a broad diversity of bacteria able to degrade EPS that can be found in carbonatogenic environments [55]. Overall, how the chemical diversity of EPS matters for the precipitation of carbonate mineral phases needs to be better assessed. In the future, this may help assessing which bacterial groups may have more impact on carbonatogenesis based on their EPS production.

There are still diverging views about how EPS are involved in carbonate precipitation. In some cases, it has been proposed that acidic polysaccharides inhibit carbonate precipitation [56, 57]. As a result, carbonatogenesis may occur in EPS-poor areas only, where high concentrations of Ca²⁺ and Mg²⁺ have been released by the degradation of polysac-charides by heterotrophic bacteria [58]. Alternatively, it has been speculated that EPS may transform upon decay into a highly organized template structure favoring calcium carbonate nucleation [46].

Giuffre et al. [40] provided a different—yet compatible—view based on a more mechanistically-proven basis. They showed that charge density is a prominent parameter to predict the impact of EPS on nucleation: the higher the charge density (e.g. presence of highly sulfated or carboxylated polysaccharides), the greater the energy barrier for nucleation. As a result, EPS with higher charge density induce higher nucleation rates at higher supersaturation indices of the solu-tion, while at lower supersaturation indices, near-neutral EPS macromolecules induce higher nucleation rates. Structural variations in EPS impact nucleation rates to a lower extent than charge densities.

Moreover, the EPS composition with respect to uronic acid, carbohydrates and protein influence carbonate polymor-phism in vitro [57], with some EPS favoring aragonite precipitation, while others favor calcite formation (Fig. 2). This might be particularly related to the identity of some small acidic proteins. These proteins, more precisely their aspartic acid residue (Asp) possibly interact with the surface of the growing precursor crystals and inhibit further transformation into the most stable polymorph, i.e., most often calcite [59]. Similarly, variations of xanthan content and the presence of acidic amino-acids were shown to impact carbonate phase polymorphism and crystal habits [60]. Consistently, Han et al. [10] recently showed that EPS produced by *Bacillus subtilis* species [10] (see Additional file 1) contain abundant acidic amino-acids such as glutamic acid and Asp, and act as nucleation sites for carbonate mineral phases in supersaturated solutions. Lastly, high molecular weight acidic proteins secreted by some bacteria were shown to influence the precipitation of diverse mineral phases including carbonates [61]. The significance of such secretions remains to be more widely assessed.

Systematically exploring the relationship between chemical and structural properties of EPS and nucleation rates as well as crystal habits and carbonate polymorphism will be an important achievement. This might depend on the chemical conditions prevailing where carbonatogenesis occurs [40]. Among the different approaches to test relationships between EPS and carbonate formation, it will be very interesting to use EPS-producing and EPS-free mutants of cyanobacterial strains [62]. Finally, dedicated metagenome analyses may also help to better assess the relationship between taxonomic diversity and EPS that may have a strong impact on carbonatogenesis.

## 2.1.2 Influence of bacterial cell walls

The cell walls of *Myxococcus xanthus* were shown to favor the precipitation of calcite and phosphate mineral phases in supersaturated solutions [63, 64]. Negatively charged functional groups in glycoproteins and proteoglycans (Fig. 2) play a role in heterogeneous nucleation but the need for some stereochemical analogy between the calcite surface and nucle-atating polymers has also been briefly suggested. Independently, calcite precipitation by *Myxococcus xanthus* can also be triggered in some cases by the supersaturation increase caused by the cell metabolic activity as further explained below [63]. The cell wall of *Bacillus subtilis*, mostly composed of peptidoglycan and teichoic acids was also shown to promote the nucleation of carbonate mineral phases. This occurred even when cells were dead or disrupted, demonstrating a clear case of biologically-influenced mineralization [65]. The exact molecular drivers were not identified but nucleating properties were kept even when the cell wall fraction was heated up to 100 °C.

Several studies have characterized the functional groups composing biomolecules at the surface of bacteria, regardless of the nature of the biomolecules [66, 67]. They have quantified their surface densities and acidity constants to model their capabilities to adsorb cations [66]. In particular, they have tracked functional groups, which are negatively charged at alkaline pH and can therefore interact with Ca²⁺. These functional groups, carried by cell bound polysaccharides but
not exclusively, are involved in passive nucleation of CaCO$_{3}$ by bacteria [68]. Carboxylate and phosphoryl functional groups likely play the major role [69–71]. In contrast, Martinez et al. [72] suggested that some bacteria may express dif- ferent functional groups at their surface such as amines, creating a positively charged surface which impedes carbonate precipitation. The precise biochemical carriers of these amine groups remain uncertain.

Surface layers (S-layers), a single layer of identical (glyco-)proteins arranged periodically and integrated within the cell walls of some bacteria, have received attention for their role in the precipitation of CaCO$_{3}$ phases by the *Synechococcus* strain GL24 [73] (Fig. 2). Mineral nucleation starts on negatively charged binding sites, which are located at a high density within the large holes of the S-layer arrays [74]. Moreover, the mineralized S-layer can be shed by the cells, feeding car- bonate deposition in lakes [73]. Despite the importance of these pioneering studies, none to our knowledge has further assessed the significance of these proteinaceous structures in bacterial carbonatogenesis.

More recently, Marvasi et al. [75] evidenced the role in carbonatogenesis of two additional types of organic mol- ecules abundantly found in some bacterial cell walls: dipicolinic acid (DPA, pyridine-2,6-dicarboxylic acid), a pyridine ring substituted by two carboxylic functional groups, and teichoic acids, polymers of glycerol- or ribitol-phosphate and carbohydrates linked by phosphodiester bonds (Fig. 2). This was achieved by the production of *B. subtilis* etfA deletion mutants impaired in their capability to precipitate CaCO$_{3}$, which possess significantly less dipicolinic acid and teichoic acid in their cell walls. Interestingly, DPA was also previously shown to be involved in very high Ca accumulation by *B. subtilis* endospores [76].

In this section, we focused on influenced mineralization mode. It is mediated by a diversity of molecules favoring heterogeneous nucleation, which operate even in metabolically inactive cells, as long as the extracellular solution is supersaturated with mineral precipitates. In the case of metabolically active cells, their impact can combine with a local increase of the solution supersaturation induced by some metabolic reactions that we discuss below, and which con- tribute to biologically induced mineralization [77].

### 2.1.3 Bacterially induced mineralization of calcium carbonates

This section focuses on bacterial metabolic activities promoting and/or speeding up the kinetics of carbonate precipita- tion by shifting extracellular chemical conditions.

Calcium carbonate precipitation is a chemical reaction that can be captured by the following equation:

$$Ca^{2+} + CO_{3}^{2-} = CaCO_{3}(K_{s}),$$

where $K_s$ is the solubility constant which depends on the structure of the CaCO$_3$ mineral phase and temperature. The activity product of the reactants, Q, is defined as: $Q = a(Ca^{2+}) \cdot a(CO_{3}^{2-})$, where a() refers to the activity of chemical species.

When Q exceeds the $K_s$ of a mineral phase, the solution is supersaturated with this phase and precipitation may ther- modynamically occur. Q depends on the concentrations of $Ca^{2+}$ and $CO_{3}^{2-}$, hence on the concentrations of $Ca^{2+}$ and $HCO_{3}^{-}$, and pH. By modifying pH, $a(Ca^{2+})$ and/or $a(HCO_{3}^{-})$, at least locally, a metabolizing bacterium can increase the saturation of a solution to a value exceeding mineral solubility, thereby rendering precipitation thermodynamically possible when it was not possible before the bacterial action. In many cases, organisms thrive in solutions, which are already saturated/ supersaturated with some mineral phases. However, in slightly supersaturated solutions, precipitation may be slow [78]. Morse et al. [79] suggest that Q should be 20–30 times higher than $K_s$ so that carbonate mineral phases precipitate spontaneously, i.e. within a few hours to a day. Under these circumstances, the increase of the solution saturation by biological activity speeds up the kinetics of precipitation and makes it effectively occurring [80].

The increase of $a(CO_{3}^{2-})$ induced by metabolic activities has been called the intrinsic ‘alkalinity engine’ [41]. The diversity of metabolic pathways contributing to this has been particularly studied in the context of microbialites. Alternatively, some bacterial processes may increase at least locally $a(Ca^{2+})$, contributing overall to the increase of Q as well.

### 2.1.4 The diversity of metabolic pathways involved in the alkaline engine

As mentioned above, any metabolic pathway increasing $a(CO_{3}^{2-})$, i.e. increasing $[HCO_{3}^{-}]$ at a constant pH and/or increasing pH at a constant $[HCO_{3}^{-}]$, favors the precipitation of carbonate mineral phases. An increasing number of studies assessing microbial diversity in microbialites aim at highlighting these metabolic pathways [55, 81].
#### 2.1.4.1 Photo- and chemo-autotrophy
In the context of microbialites, photolithoautotrophs and in particular cyanobacteria have received thorough attention for their involvement in carbonate biomineralization. Indeed, many cyanobacteria tend to increase the pH of extracellular solutions that are not highly pH-buffered. Overall, this is due to their propensity to fix CO₂ using RuBisCO after bicarbonate ions (HCO₃⁻) uptake. Indeed, this results in a net consumption of protons by the cells, hence an increase of extracellular pH [82]. There may be no net proton consumption by cyanobacteria and therefore no impact on carbonatogenesis, in environments where enough CO₂ is available because CO₂ might be preferentially used over HCO₃⁻. However, while high environmental pCO₂ may have been common on the Precambrian Earth [20, 83], this is usually not the case in most modern aqueous environments. In that case, inorganic carbon acquisition mostly involves the active uptake of HCO₃⁻. A complex set of molecular pathways increases HCO₃⁻ uptake, storage and conversion to CO₂ within the cells. Altogether, these pathways are referred to as CO₂-concentrating mechanisms (CCM) (Fig. 3). There is a broad diversity of molecular actors involved in CCM among the diverse lineages of cyanobacteria [84, 85]. More specifically, HCO₃⁻ ions are imported against their concentration gradient by a diversity of transporters, such as through a symport with Na²⁺ or by ATP hydrolysis [85]. The expression of some of these transporters-encoding genes is constitutive, while it is induced for some others when CO₂ fugacity is low enough. This shows that CCM can partly respond to environmental conditions and that inferring the genetic capabilities of strains might not be enough to predict their role in carbonatogenesis.

Bicarbonate is transformed into CO₂ by carbonic anhydrases (CAs) within microcompartments delimited by protein shell, called carboxysomes. This releases hydroxyl ions, which are buffered by the import of H⁺ for the sake of intracellular pH regulation. As a result, extracellular pH and therefore the extracellular solution saturation increase with regard to carbonate mineral phases [20]. Moreover, it has been suggested that part of the H⁺ import can be achieved by antiport with Ca²⁺ ions by Ca²⁺/H⁺ exchangers. This further increases extracellular solution saturation with calcium carbonate mineral phases [86]. A link between a Ca²⁺/H⁺ exchanger and an HCO₃⁻ transporter has been shown in *Synechocystis* PCC 6803. Indeed, the inactivation of the single Ca²⁺/H⁺ exchanger (slr1336) found in this organism significantly increased the calcification rate and stimulated the expression levels of CCM-related genes, especially *cmpA*, which encodes the BCT1 HCO₃⁻ transporter [87].

Carbonic anhydrases are Zn(II)- and/or Fe(II)- or Co(II)-) metalloenzymes catalyzing the reversible hydration of CO₂, i.e. the interconversion of CO₂ and HCO₃⁻. Therefore, they participate in pH regulation, and inorganic carbon handling. They have been intensively studied in the context of CO₂ capture and sequestration [88, 89]. While they have been shown to be major players in eukaryotic calcification [90], their role in bacterial carbonatogenesis often remains neglected [91]. CAs are ubiquitous in the kingdom of Bacteria and diverse, belonging to three families (α, β, γ), which may have evolved from a γ-like ancestral CA [92–94]. A broad structural diversity occurs amongst CAs, which can exist as monomers or

<figure><img src="image_7.png" /><figcaption>**Fig. 3** Simplified model of CCM-dependent biologically induced mineralization of CaCO₃. Carbon concentrating mechanism CCM involves the uptake of HCO₃⁻. Carbonic anhydrase (CA) converts CO₂ into HCO₃⁻ in the cytoplasm and HCO₃⁻ into CO₂ in the carboxysome. CO₂ is fixed by RubisCO and used by the Calvin-Benson-Bassham Cycle (CBBC). Conversion of HCO₃⁻ into CO₂ releases OH⁻. To buffer intracellular pH, specific transporters such as Ca²⁺/H⁺ antiporters import H⁺ and export Ca²⁺. The import of H⁺ inside the cell lead to an increase of the extracellular pH, which together with export of Ca²⁺ favors the precipitation of extracellular CaCO₃ [20, 30, 172]</figcaption></figure>
polymers, coupled with a low sequence similarity [94]. Cell localization is also diverse, some CAs being intracellular, intra-organellar and others periplasmic or extracellular [93]. How this localization may control their capability to induce carbonatogenesis remains to be determined.

A recombinant γ-carbonic anhydrase from *Serratia* sp. expressed and purified in *E. coli* was capable to promote the precipitation of calcite. Its activity was optimal at near-neutral pH (7.6–8.0) at 4 °C [95]. Moreover, some CAs were shown to be more efficient than others at inducing CaCO₃ precipitation [37, 96]. Overall, targeting CAs seems important to decipher the carbonatogenic capabilities of a strain but further work is needed to understand what controls the carbonatogenic efficiency of CAs and if some specific CAs families should be more particularly targeted.

However, many uncertainties remain about the prime molecular mechanisms involved in carbonatogenesis by auto-
trophs and the way to predict their carbonatogenic capabilities. First, are there cyanobacteria harboring particularly
effective CCMs that may therefore strongly impact carbonatogenesis? Second, which part of intracellular pH regulation
is achieved by the functioning of Ca²⁺/H⁺ exchangers vs other H⁺ transporters in calcifying cyanobacteria? Third, there
is still some debate about the way cyanobacteria impact carbonatogenesis: do they induce carbonatogenesis through
their metabolic activity as stressed on in the above lines [97, 98], or do they influence it through inactive heterogeneous
nucleation (e.g. biologically influenced mineralization, see above). In the latter case, molecules at cell surface would
play a dominant role [68]. One possible explanation for the conflicting conclusions of these studies is that experiments
maintaining relatively high pCO₂ conditions [68] may undermine CCM effects and therefore favor heterogeneous nuclea-
tion over biologically induced mineralization. Last but not least, it should be noted that some endolithic cyanobacteria,
despite having the same molecular apparatus as described above, are known to induce CaCO₃ dissolution instead of
precipitation [99]. Deciphering the molecular reasons that make a cell dissolve or precipitate CaCO₃ will be crucial in
the future. In that regard, Zhu et al. [100] showed that the non-photosynthetic bacterium *Brevibacterium linens* BS258
can induce precipitation or dissolution of CaCO₃ depending on extracellular Ca²⁺ concentration and the subsequent
variations in CA genes expression.

Finally, the relationship between the metabolic activity of cyanobacteria and the induction of carbonate mineral
phase precipitation may be generalized to all other bacteria using the CO₂-assimilating Calvin-Benson-Bassham cycle
and also to all other autotrophic carbon fixation pathways such as the reductive citric acid cycle, the Wood-Ljungdahl
pathway, and the dicarboxylate/4-hydroxybutyrate cycle [101], at least when cells both incorporate bicarbonate and
fix CO₂. Although some studies have stressed on the role of anoxygenic phototrophs in carbonate precipitation, e.g.
in microbialites [8, 102], the assessment of the contribution of this much broader bacterial (and archaeal) diversity to
carbonatogenesis and how CCM might be widespread and effective in them remain poorly explored.

2.1.5 Other carbon-based metabolic pathways

**2.1.5.1 Methanotrophy** Anaerobic methane oxidation has been shown as a pathway inducing the precipitation of sig-
nificant amounts of carbonate mineral phases with low δ¹³C [103]. In contrast, aerobic methane oxidation should favor
carbonate dissolution since it releases CO₂ [104]. However, some studies have shown that the so-generated CO₂ with low
δ¹³C may be re-sequestered within Mn- and/or Fe-rich carbonates under particular conditions [105]. Anaerobic-meth-
ane oxidation can be coupled by some Bacteria and/or Archaea with the reduction of sulfates (in consortia associating
Archaea and Bacteria), nitrates, nitrites, humic acids, iron or manganese [106]. Bacterial methanotrophs can be detected
based on genes coding for the particulate (pmoA) or soluble (mmoX) forms of the methane monooxygenase [106], while
Archaeal methanotrophs can be detected by the presence of the mcrA gene encoding methyl co-enzyme reductase.

**2.1.5.2 Fatty acid catabolism** Three mechanisms are proposed to mediate bacterially-induced carbonate mineralization in *B. subtilis*. One occurs through ureolysis (see below), whereas the other two take place in urea-free growth media through the production of dipicolinic and teichoic acids (see above) or in connection with the fatty acid metabolism [107, 108]. Several mutants impaired in their ability to precipitate calcite in urea-deprived growth media highlighted the involvement of several operons including *yus*, *yko* and *eft* genes [107]. The *lcfA* operon composed of five genes (*lcfA*, *fadR*, *fadB*, *etfB*, and *etfA*) received particular attention. *etfA* encodes a putative flavoprotein possibly involved in the metabolism of fatty acids, which appeared to be essential for calcite precipitation in the absence of urea [108]. It was suggested that the loss of calcite precipitation in the *eftA* mutants was due to a higher extracellular release of H⁺ no longer consumed by the fatty acids metabolism as compared to the wild-type strain, which favors extracellular CaCO₃ precipitation [109]. In a later study, Marvasi et al. [68] suggested that the *eftA* mutation may also change the phospholip-

<figure><img src="image_8.png" /></figure>
ids membrane composition, which therefore may have a role in calcite precipitation. Overall, it becomes apparent that *B. subtilis* can induce CaCO₃ precipitation through various mechanisms depending on the culture conditions.

### 2.1.5.3 Ureolysis
Bacterial ureolysis has also received particular attention, especially from studies with applied purposes since it seems capable of inducing massive carbonate precipitation [37, 110, 111]. The hydrolysis of urea (CO(NH₂)₂) releases ammonium (NH₄⁺), HCO₃⁻ and hydroxyls, the two latter contributing to the high increase of solution saturation with carbonate phases, hence their precipitation. Ureolysis primarily depends on the activity of the urease with some additional role of cell surfaces in the heterogeneous nucleation of the mineral phases [112]. Consistently, strong carbonatogenesis capabilities could be conferred to the *E. coli* HB101 strain harboring a plasmid encoding *Bacillus pasteurii* (*Sporosarcina pasteurii*) urease genes [113]. Similarly, mutants of *S. pasteurii* with urease activities higher than the wild type strain produced more calcite [114].

Ureases can be found in a wide diversity of organisms, including a broad diversity of bacteria [112, 115]. Almost all ureases are cytoplasmic and can constitute up to 1% of the cell dry weight in *S. pasteurii* [113]. However, ureases can also catalyze ureolysis extracellularly, and it has been reported that soil matrix-bound ureases can represent significant players of ureolysis [116].

Bacterial ureases are high molecular weight, cysteine-rich enzymes belonging to the superfamily of amidohydrolases and phosphotriesterases [117]. Genes coding for the three subunits (*ureA*, *ureB* and *ureC*) as well as accessory proteins (UreF, UreH, and UreG, involved in the nickel active site assembly) can be tracked in genomes [100, 110]. Moreover, the *ureC* gene, encoding the well-conserved subunit harboring the nickel catalytic active site has been used as a marker gene that can be tracked by molecular approaches [115].

Some structural diversity among bacterial ureases may be associated with variations in affinities for substrates [112] and pH of optimal activity [117].

Carbonate precipitation by ureolytic bacteria follows a Michaelis–Menten kinetics with ureolysis as the rate-limiting step [118]. Carbonatogenesis rates are therefore organism-dependent since they depend on the activity of the intrinsic urease [119]. Moreover, when ureolysis occurs intracellularly, the efficiency of cell transmembrane transport of urea by urea permeases, of ammonia, protons and Ni by transporter systems matter for the overall carbonatogenesis capabilities of the cells [113]. Finally, it has been suggested that synergies exist between the functioning of ureases and CAs in addition to their direct contribution to calcification, since, e.g. Ni incorporation in urease is dependent on the intracellular CO₂/HCO₃⁻ ratio, which is regulated by CA [91].

Ureolysis activity can also be affected by external environmental conditions, including pH [111], urea concentration and temperature [118]. For example, a pH increase can favor in some soils the growth of urease-producing microorganisms, resulting in an increased number of *ureC* detected copies and an increased ureolysis rate [120]. In contrast, oxygen fugacity does not have a direct impact on urease activity, but anoxic conditions may impede the growth of aerobic strains, therefore urease production, hence carbonatogenic activity [118]. Some authors have suggested that the activity of urease can be modulated by calcium concentration [112]. Zhu et al. [100] showed in the marine Actinobacterium *Brevibacterium linens* BS258 that an increase of Ca²⁺ upregulated the expression of genes encoding peptide/nickel ABC transporters which boosted urease activity. Whether this correlation is systematically positive is yet to clarify.

Ureolytic bacteria are found in a wide range of environments [115]. Interestingly, it has been suggested that ureolysis may play a role in the formation of some microbialites, based on the metagenomics detection of urea ABC transporters, and ureases, dominated by genes of Proteobacteria [81].

### 2.1.5.4 Other nitrogen-related metabolic pathways
Nitrate reduction and ammonification of amino-acids are prone to induce the precipitation of CaCO₃ [121, 122]. Some of these processes are dissimilatory, i.e. reduction provides ATP to the cell, while others are assimilatory, i.e., they serve as a source of nitrogen for biosynthesis. Interestingly, nitrate reduction has been highlighted by Drew [123] as an important mechanism in marine carbonate precipitation already a century ago, but its significance in modern CaCO₃ precipitation remains poorly assessed.

Denitrification consists in the reduction of NO₃⁻ (nitrate) to N₂ through several intermediates, including nitrite (NO₂⁻), nitric oxide (NO), and nitrous oxide (N₂O). Each step of denitrification is catalyzed by various enzymes harboring diverse metal cofactors. The first one is performed by either periplasmic or cytoplasmic nitrate reductases, encoded by *napAB* and *narGHL* genes, respectively [124–126]. Overall, more than 50 genes in a genome are encoding the core structures of the denitrification apparatus [124]. Nitrate reduction encompasses a larger group of bacteria, some of which proceeding through the first NO₃⁻ → NO₂⁻ step only.
Nitrate is used as an electron acceptor in anoxic environments by bacteria that are often facultative nitrate reducers. Nitrate reduction can be coupled by diverse heterotrophic bacteria with the oxidation of organic C but also by some autotrophic bacteria with alternative electron donors including H₂ and reduced S, As and Fe chemical species [127]. Depending on the electron donor, nitrate reduction consumes (e.g. organic C, H₂, HS⁻), or produces (e.g. S₂O₃²⁻, Fe²⁺, Mn²⁺) protons, the former favoring the induction of CaCO₃ precipitation. In the case of heterotrophic denitrification, the additional production of CO₂ may further impact saturation with CaCO₃. For example:

$$4\text{NO}_3^- + 5\text{CH}_2\text{O} + 4\text{H}^+ \rightarrow 2\text{N}_2 + 5\text{CO}_2 + 7\text{H}_2\text{O}.$$

Denitrification is distributed among a highly polyphyletic group of bacteria [127]. Therefore, the targeting of functional genes such as *narGHL* and *napAB* (encoding the respiratory and periplasmic nitrate reductases respectively) instead of 16S rDNA genes has been used to look for nitrate-reducing bacteria in the environment [128].

Nitrate reduction has been studied as a driver of bacterially-induced carbonate precipitation in several geotechnical applications [121] but also in environmental microbial mats where calcification occurs [129]. Although it seems to have a lower efficiency than ureolysis for carbonatogenesis [122], nitrate reduction has been proposed as an alternative pathway for applications where the production of ammonium would be unwanted and/or where wastewater with elevated nitrates concentrations are available [130]. Nitrate reduction may even supplement ureolysis in some cases, and improve CaCO₃ precipitation efficiency [130]. High Ca²⁺ concentrations and urea have been reported to decrease nitrate reduction rates [130], but this effect will need to be assessed more systematically. Heterotrophic denitrification was reported to be optimal at a pH around 7–8 but it can also occur at higher pH [131, 132]. Many other parameters may affect denitrification rates including salinity, limitation of metal cofactors, phosphorus availability [133]. Lastly, Karatas [133] concluded that CaCO₃ precipitation is also controlled by the ratio of the alkalinity production (consumption of H⁺) to CO₂ production. This ratio depends on the oxidation state of C and the N content in the electron donor. Overall, *Pseudomonas aeruginosa* and *Diaphorobacter nitroreducens* are known to promote precipitation via denitrification metabolism [134] (see Additional file 1).

Ammonification refers to diverse processes resulting in the hydrolysis of nitrogenous organic matter and the release of ammonium. This includes the already discussed ureolysis and reduction of nitrate into ammonium. In addition, ammonification of proteins or amino-acids, among others, under oxic conditions was also shown to produce ammonium, sometimes CO₂ and to consume H⁺ [135]. Therefore, ammonification is capable of inducing CaCO₃ precipitation [37] following the general equation:

$$R-NH_2 + H_3O^+ \rightarrow R-OH + NH_4^+$$

It involves multiple steps of protein, peptide and amino acid decomposition by a diversity of enzymes, including proteases, hydratases, peptidases, and deaminases. Reyes et al. [136] provided a list of 47 enzymes retrieved from metagenomes of marine sediments, possibly involved in ammonification. The corresponding genes were among the most abundant genes involved in nitrogen metabolism. Recently, using the model bacterium *Alcanivorax borkumensis*, Krause et al. [137] proposed that bacterial ammonification might be an important driver of carbonate formation in shallow costal marine environments. Similarly, Rodriguez-Navarro et al. [138] showed the capability of *Myxococcus xanthus* to induce CaCO₃ biomineralization by performing oxidative deamination of amino acids.

#### 2.1.5.5 Sulfur-related metabolic pathways
Many studies have highlighted the role of sulfate-reducing bacteria (SRB) in CaCO₃ precipitation in anaerobic environment. Braissant et al. [139] enumerated several processes mediated by SRB that favor CaCO₃ precipitation: sulfate reduction consumes protons and therefore increases the saturation of the extracellular solution. SRB also increase Ca²⁺ activity. Indeed, the degradation of Ca²⁺-complexing carboxylic acids by SRB may locally release Ca²⁺. Moreover, by removing sulfates ions, they also relieve the kinetic inhibition of dolomite precipitation. Last but not least, the EPS produced by SRB may favor influenced mineralization. However, SRB may alternatively decrease the pH of the extracellular solution to an extent that depends on the electron donors used, so that the positive impact of SRB on CaCO₃ precipitation should not be considered as systematic [140]. Moreover, Aloisi [141] suggested that SRB may have a limited impact on the increase of saturation with CaCO₃, in comparison with oxygenic photosynthesis. In contrast, SRB have been advocated as important players in the formation of the modern microbialites in the Bahamas [142]. Whether this is due to their metabolic activity or influenced mineralization remains to be clearly assessed.

Sulfide oxidation has sometimes been considered as a metabolism promoting CaCO₃ dissolution [41]. This actually depends on the nature of the reactants (sulfides vs. sulfur vs. thiosulfate) and the ultimate byproducts (sulfur vs.
thiosulfates vs. sulfates). Several sulfoxidizing pathways consume protons and may induce CaCO₃ precipitation. Consistently, Himmler et al. [143] proposed that nitrate-driven sulfide oxidation may be involved in the formation of deep stromatolite-like deposits.

## 2.2 Biologically induced mineralization by metabolic modifications of extracellular calcium activity

As already mentioned, the activity of Ca²⁺ ions also controls the saturation of a solution in respect with CaCO₃ phases. Calcium is known to be involved in membrane transport mechanisms, photosynthesis, cell division, cell differentiation processes such as sporulation, and heterocyst formation [144]. The intracellular Ca²⁺ concentration is tightly regulated at a low value, in the submicromolar range. In the environment, calcium concentration is usually up to 10³ times higher than intracellular concentration [145]. Therefore, Ca²⁺ enters into the cell via a passive influx. In contrast, the efflux of Ca²⁺ is usually active, involving different types of pumps, including ATPases and Ca²⁺/H⁺ exchangers. At these locations, the local extrusion of Ca²⁺ may favor CaCO₃ precipitation by an increase of Ca²⁺ activity, sometimes coupled with a decrease of H⁺ activity [146]. Castanier et al. [33] and McConnaughey and Whelan [147] consistently proposed that Ca²⁺ extrusion might induce CaCO₃ precipitation. Partly supporting this hypothesis, Banks [148] showed that the knock-out mutant of the *chaA* gene encoding the Ca²⁺/2H⁺ antiporter of *Salmonella typhimurium* has lost its capacity to grow on media containing calcium and thus they suggested that calcification might be involved in Ca detoxification. However, the significance of this process in induced (i.e., non-controlled) mineralization remains to explore.

### 2.2.1 A particular case of induced CaCO₃ mineralization: viral lysis

CaCO₃ precipitation may be induced in some cases by the viral lysis of cyanobacterial cells. This case may not be strictly considered as biologically-induced mineralization since it occurs when cells burst and die. Yet, it involves a local chemical shift created by the cells. A theoretical model was developed based on the following rationale [149]: cyanobacteria use active processes to highly concentrate bicarbonates intracellularly compared to the surrounding solution. Overall, this intracellular dissolved inorganic carbon may represent up to 1 Gigaton in the whole ocean [149]. When the intracellular content is fully released, e.g. by cell rupture following a viral infection, the local increase of bicarbonate concentration in an already supersaturated solution, such as seawater, may be high enough to overcome the energy barrier of homogenous nucleation. As a result, CaCO₃ precipitation occurs at once without the involvement of the cell surface. In contrast, homogenous nucleation is kinetically limited in seawater in the absence of viral lysis and never occurs. Only alternative processes such as heterogeneous nucleation on EPS and/or microbial cell surfaces or increase of saturation by metabolic activity would otherwise allow the precipitation of CaCO₃. In their model, Lisle and Robbins [149] assessed the outward diffusion of bicarbonate molecules and the inward diffusion of Ca²⁺ ions after the cell burst and showed that the formation of vaterite became highly favorable in a limited yet significant volume around the cells. They postulated that this process might result in a significant source of carbonate sediments, when it occurs during bloom events with enhanced viral lytic events, possibly in the so-called whiting events. More recently, Xu et al. [150] conducted laboratory experiments to test this model. Consistently, they observed that cyanobacterial cells of *Synechococcus* sp. PCC 7177 incubated in seawater induced the precipitation of brucite (Mg(OH)₂), whereas after phage infection, lysed cells seemed to favor amorphous calcium carbonate (ACC) and aragonite precipitation.

## 2.3 Bacterially controlled mineralization of calcium carbonates

Determining the control of mineralization by bacteria is not straightforward. Indeed, it ultimately requires to characterize specific genes and the involved molecular mechanisms. Therefore, we may ignore some cases of controlled mineralization of CaCO₃ even among well-studied model bacteria. Presently, there are two accepted cases of bacterially controlled mineralization of CaCO₃: CaCO₃ formation by *Achromatium* sp. and ACC formation by several cyanobacteria. These cases have several peculiarities compared with the induced and influenced mineralization pathways: (i) mineralization is intracellular; (ii) it can occur under apparently out-of-equilibrium conditions, i.e. in extracellular solutions undersaturated with CaCO₃ mineral phases. This biomineralization results from an energetically costly creation of intracellular supersaturated conditions; (iii) Relatedly, the chemical composition and structure of the resulting biominerals may be different from those expected when considering the chemical conditions prevailing extracellularly. Hence, they may be true markers of biogenicity in these environments.
The first evidence of controlled biomineralization was found in *Achromatium*, a genus affiliated to the Gammaproteobacteria. It was described by Schewiakoff as early as 1893 [4]. Presently, all known *Achromatium* species can form intracellular CaCO₃ [151]. They are found at the oxic/anoxic boundary in freshwater, brackish or marine sediments [152, 153]. These giant bacteria with cells measuring up to 100 µm in length, contain large (5–6 µm) inclusions of intracellular CaCO₃, which sometimes occupy most of the cell volume. They also contain small (0.5–2 µm) sulfur (S⁰) inclusions [153]. Some *Achromatium* cells were observed to contain intracellular CaCO₃ granules in extracellular solutions undersaturated with all CaCO₃ phases [151].

The underlying molecular mechanisms are difficult to study because there is no cultivated representative of *Achromatium*. However, several observations have provided hints. First, some TEM observations have suggested a membrane around CaCO₃ inclusions [151, 153]. However, this feature is not obvious on published pictures. Recently, Schorn et al. [154] intriguingly suggested that CaCO₃ granules in *Achromatium* may actually form within invaginations of the cytoplasmic membrane, i.e. within the periplasm. If true, this would greatly contradict the current model of CaCO₃ formation by *Achromatium*. This clearly shows that significant work remains to be achieved on this system. Then, the usual three ingredients for CaCO₃ mineralization, i.e. Ca²⁺ and HCO₃⁻ provision as well as proton consumption, have been assessed. First, the formation of intracellular CaCO₃ granules requires a relatively large amount of Ca, although Ca is usually poorly available within cells. Thus, CaCO₃ biomineralization by *Achromatium* may involve a specific active mechanism for concentrating Ca from the environment. Moreover, Salman et al. [152] evidenced an intracellular compartment overlooked so far, which may contain high concentrations of free Ca²⁺ and where CaCO₃ precipitation may start. The detection of genes encoding a plasma membrane Ca²⁺-ATPase (PMCA-like) and a Na/H⁺-Ca exchanger (yrbG), and several genes encoding pyrophosphatases and vacuolar (V)-type ATPases in the draft genomes obtained by single-cell genome sequencing was discussed in that regard [155]. Second, the provision of HCO₃⁻ may be achieved in some *Achromatium* populations by CCM. Carbonic anhydrase encoding genes were detected in *Achromatium* draft genomes [155]. However, a gene encoding a RuBisCO large subunit (rbcL) was detected in *Achromatium* populations from Rydal Water but not in those from Hell Kettle [153]. Similarly, some diversity regarding the capability to grow autotrophically was observed among various *Achromatium* populations: some were able to assimilate carbon from bicarbonate, while others needed organic C such as acetate [151]. Finally, a correlation has been observed between variations of environmental O₂ fugacity and the amount of sulfur and CaCO₃ inclusions within the cells. This led Salman et al. [152] and Yang et al. [156] to propose that intracellular CaCO₃ accumulates upon oxidation of sulfide to elemental sulfur, a redox process consuming protons and occurring mostly under anoxic conditions. In contrast, CaCO₃ dissolves upon oxidation of sulfur to sulfate, a redox process releasing protons and occurring under oxic conditions (Fig. 4). As a result, CaCO₃ formation would be directly related with the sulfur metabolism of the bacteria and serve somehow as a pH buffer. Accordingly, genes involved in oxidation of sulfide to sulfur and sulfur to sulfate were detected in the draft genomes of *Achromatium*. The increasing number of molecular studies published lately about *Achromatium* holds great promises for soon unravelling more completely the mechanisms of CaCO₃ biomineralization by this bacterium.

For a long time, it has been believed that only *Achromatium* performs controlled mineralization of CaCO₃ among bacteria until the recent discovery of a similar process in some cyanobacteria. This was first discovered in *Gloeomargarita lithophora*, a deep-branching cyanobacterium isolated from modern carbonate deposits forming in the highly alkaline (pH ~ 8.9) Lake Alchichica, Mexico [157, 158]. *G. lithophora* is the closest extant relative of plastids [159]. Cells harbor intracellular amorphous Ca-Mg-Sr-Ba-carbonates granules distributed within the whole cell [157]. There are 21 ± 5 inclusions per cells on average, occupying ~ 6% of the total cell volume and these inclusions measure 270 ± 44 nm in diameter. Fifteen additional cultivable cyanobacterial strains were later found to form intracellular ACC (iACC) [43, 160] (see Additional file 2). Some of these strains had been extensively studied before, including by TEM, supporting the idea that they have been overlooked. This suggests that we may still have an incomplete assessment of the diversity of bacteria forming intracellular CaCO₃. Cyanobacteria forming iACC are phylogenetically-distant and live in diverse biotopes such as alkaline lakes, hot springs, or soils [43, 161]. All the members of the *Thermosynechococcus elongatus* BP-1 clade, shared the ability to form iACC showing that this biomineralization process is heritable, and might be ancient [43]. Moreover, two types of intracellular distributions of the inclusions were observed among the 16 iACC-forming strains known so far: iACC granules were scattered throughout the cells in 11 cyanobacteria, whereas they were located at cell poles and septa in five other cyanobacteria [14, 43, 160, 162] (Fig. 5). In the latter group, iACC formation is therefore obviously related with cell division. Overall, this questions whether a single pathway is involved or iACC biomineralization appeared several times by convergence during evolution in cyanobacteria.

Similar to *Achromatium*, the molecular mechanisms involved in cyanobacterial iACC biomineralization remain poorly known. However, several observations allow discussing hypotheses. First, in cyanobacteria where ACC formation starts

<figure><img src="image_9.png" /></figure>
<figure><img src="image_10.png" /><figcaption>**Fig. 4** Intracellular CaCO₃ precipitation and the dissolution within *Achromatium* cells. Protons are consumed when sulfides (H₂S) are oxidized to elemental sulfur under anoxic conditions. This leads to an increase of pH and precipitation of CaCO₃ (grey ellipsoid). Protons are released when elemental sulfur (S) is oxidized to sulfates (SO₄²⁻) under oxic conditions. This results in the decrease of pH and dissolution of CaCO₃. Some debate exists about the localization of CaCO₃ precipitation: Head et al. [153] and Gray and Head [151] suggested that intracellular CaCO₃ are enclosed within an intracellular compartment delimited by a lipid bilayer (black line). In contrast, Schorn et al. [154] suggested that CaCO₃ forms within an invagination of the cytoplasmic membrane (blue line), open to the periplasm</figcaption></figure>

<figure><img src="image_11.png" /><figcaption>**Fig. 5** Transmission electron microscopy images in the bright field mode of ACC-forming cyanobacteria: a *Cyanobacterium* sp. PCC 7425 cells showing ACC inclusions (darkest inclusion) scattered throughout the cells; b *Synechococcus lividus* PCC 6716 cells showing ACC inclusions (darkest inclusions) located at the septum and the poles of the cells [43]. Some brighter inclusions are also visible in both strains and correspond to polyphosphate granules. Identification of polyphosphate and ACC granules was achieved by Benzerara et al. [43] based on STEM-EDX mapping, electron diffraction and XANES spectroscopy at the C K-edge and Ca L₂,₃-edges</figcaption></figure>

at the cell septum, the involvement of a Ca²⁺ influx and nucleation of ACC on the cell division FtsZ protein have been suggested as potential mechanisms [14]. In cyanobacteria where iACC granules were scattered throughout the cells, iACC granules were sometimes aligned and showed facets [14]. Moreover, they showed a tight spatial association with polyphosphate inclusions in *G. lithophora*, similar to the tight association sometimes observed between polyphosphates and carboxysomes. Overall, based on these observations and since the conversion of bicarbonate ions to CO₂ in carboxysomes might be a driver of ACC precipitation, they suggested that ACC precipitation may start on carboxysomes.
However, this was not confirmed by TEM observations. TEM analyses of samples prepared by conventional ultramicrotomy are not appropriate since iACC dissolves very fast upon sample processing [14]. Blondeau et al. [162] solved the problem by using cryo-electron microscopy of vitreous section (CEMOVIS). As a result, they showed that iACC inclusions were systematically surrounded by an electron dense envelope measuring ~2.5 nm in thickness, i.e. about half of the thickness of a lipid bilayer. The exact nature of this envelope, either proteinaceous or a lipid monolayer remains unknown. However, this suggests that ACC forms within an intracellular compartment where pH and/or Ca²⁺ and CO₃²⁻ concentrations might be much higher than in the cytoplasm. Moreover, confinement in such a compartment may explain the stabilization of the amorphous structure of CaCO₃ [163]. The observation of such a compartment, where chemical conditions might be different from those prevailing in the cytoplasm reconciles our knowledge about the conditions necessary for ACC precipitation and the observation of iACC in cyanobacteria. Indeed, ACC precipitation is thermodynamically impossible under the chemical conditions that are believed to exist within the cytoplasm of cyanobacteria [164]. Similarly, with what has been proposed for *Achromatium*, an active uptake of Ca²⁺ may feed iACC biomineralization. This active transport may be at least partly at the origin of the energy cost of this biomineralization pathway and explain how cyanobacteria can form ACC in otherwise undersaturated extracellular solutions [165]. Following the same line of a strong connection between ACC biomineralization and a particular Ca homeostasis, De Wever et al. [160] showed that iACC-forming cyanobacteria incorporate much more Ca than other cyanobacteria. Moreover, some of the iACC-forming strains require higher Ca concentrations to grow well. However, no molecular actor has been identified yet which might be exclusively shared by all iACC-forming cyanobacteria. Genes coding for Ca²⁺/H⁺ exchangers, and diverse calcium/cation exchangers, all involved in Ca active transport, have been found in all studied genomes of iACC-forming cyanobacteria [160]. Genes coding for a mechanosensitive channel have also been found in all these genomes. However, those genes can also be found in bacteria not forming ACC [160]. In contrast, genes coding for Ca²⁺-ATPases, Pit and Ca²⁺/Na⁺ antiporters were found in some but not all iACC-forming cyanobacteria.

Finally, a preferential uptake of the heaviest alkaline earth elements (Ba > Sr > Ca > Mg) was observed in *G. lithophora* but not in several other iACC-forming cyanobacteria, suggesting that it involves additional molecular mechanisms [166]. Such a preferential uptake explains why the Ba/Ca and Sr/Ca atom ratios of the intracellular carbonates in *G. lithophora* cells were higher by factors of 1370 and 86 than those prevailing in their extracellular solution. This preferential uptake is interesting since it may have a significant impact on the geochemical cycles of trace alkaline earth elements [167] and may be used to remediate pollutions by radioactive isotopes [15].

As mentioned above, because of the poor stability of ACC and based on recent teachings from the discovery of iACC by cyanobacteria, we may have overlooked a part of the bacterial diversity able to control intracellular biomineralization of CaCO₃. Recent field studies have observed non-identified organisms forming intracellular CaCO₃ [168]. Exploring further this diversity may help to better understand the molecular mechanisms involved in this biomineralization process.

## 3 Conclusions

A large diversity of mechanisms is involved in the biomineralization of carbonates by bacteria. Biological mediation may involve molecules favoring mineral nucleation by changing the interfacial energy between the extracellular solution and a nascent nucleus (influenced mineralization). Another mode of mediation, compatible with the first one, involves some bacterial metabolic activities. These activities impact the pH and/or the concentrations of bicarbonate and/or Ca²⁺ ions of the extracellular solution where cells thrive. Lastly, controlled biomineralization follows similar molecular processes but is distinguished based on the fact that it involves dedicated genes.

In this review, we focused on the diversity of mechanisms involved in bacterial CaCO₃ biomineralization. An alternative and yet related question is that of the biological function of this process. The existence of a function in the case of induced and influenced mineralization has been debated. Simkiss [169] proposed that the intracellular precipitation of Ca-rich mineral phases might be a way to detoxify cells from Ca and contribute to the maintenance of low intracellular Ca contents. Following this line, Anderson et al. [170] postulated that extracellular formation of CaCO₃ might similarly have a role in cell Ca homeostasis by detoxifying extracellular Ca excess. The idea that the bacterial mediation of extracellular CaCO₃ precipitation may serve the Ca homeostasis of cells and protect them in Ca-rich environments was repeatedly reaffirmed, including by, e.g. Banks et al. [148]. The way this may be understood is that cells, by lowering the apparent solubility of CaCO₃ either by heterogeneous nucleation and/or shifting pH and/or [HCO₃⁻] may lower the activity of Ca²⁺. However, the statement by Simkiss [169] that these precipitates are 'highly insoluble deposits' should be put in perspective. Ca²⁺ activity never becomes null because of the formation of these precipitates but equal to a value imposed by the

<figure><img src="image_12.png" /></figure>
equilibrium between the solution and the precipitated Ca-mineral phase. For example, in the case of iACC biomineraliza-
tion by cyanobacteria, the expected value of Ca²⁺ concentration at equilibrium with ACC when pH is 7.9 and [HCO₃⁻] is
30 mM would be 441 µM, i.e. much higher than the necessary 100 nM regulated by cell homeostasis. This questions the
role of these biominerals in this process. Obst et al. [171] did not suggest a real advantage provided to the cells by CaCO₃
precipitation but instead postulated that the stabilization of ACC was a protective mechanism against the formation of
unwanted crystalline CaCO₃. Schultze-Lam et al. [73] proposed that the localization of CaCO₃ precipitation on protein
S-layers that can later on be shed, may serve as a mechanism against cell encrustation. Overall, we note that there may be
no function at all for the cell in the case of induced or influenced mineralization. Looking systematically for one function
results from an adaptationist (or Panglossian) view. While we acknowledge that selection is a fundamental process, the
mineralization of CaCO₃ may be a secondary byproduct of other processes, the latter being the ones that are selected
for any other reason.

For controlled mineralization, several functions have been suggested: for cyanobacteria it has been proposed that (i) iACC alters the buoyant density of cells and may serve as a ballast [14]; (ii) iACC may serve as a storage of inorganic C for photosynthesis and/or to maintain relatively constant C/N ratios within the cells [110]; (iii) alternatively, carbonate precipitation may act as pH buffer during photosynthesis [157]. Similar hypotheses have been formulated for *Achromatium*, including a role of pH buffer during redox reactions affecting the different sulfur species. Controlled mineralization of carbonates has often been described in eukaryotes. Only a future detailed understanding of the molecular processes and actors involved in bacterial biomineralization will help to infer whether some of them might be shared between prokaryotes and eukaryotes and how they evolved throughout life history.

**Acknowledgements** We thank two anonymous reviewers for insightful comments.

**Authors' contributions** Conceptualization: KB, SG, FSP, FC, CCC; Writing original draft preparation: SG, KB, CCC; Writing—review and editing: SG, KB, CCC, FSP, FC, MG; Figures: SG, CCC, KB. Funding acquisition: KB. All authors read and approved the final manuscript.

**Funding** This work was supported by the French National Research Agency (HARLEY: ANR-19-CE44-0017), which supported discussion meetings and work about intracellular biomineralization and the Action Transversale du Muséum (ATM, MNHN), which supported the acquisition of microscopy data on intracellular biomineralization and publication fees. Sigrid Görgen's PhD was supported by the program Interfaces pour le vivant from Sorbonne University.

**Availability of data and materials** Not applicable.

**Ethics approval and consent to participate** Not applicable.

**Consent for publication** Not applicable.

**Competing interests** Not applicable.

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