Apr 09, 2019

Version 2

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Zymoclean™ Gel DNA Recovery V.2

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Version 1 is forked from [1. RNA isolation for tissue](https://doi.org/10.17504/protocols.io.zx4f7qw)

DOI

https://dx.doi.org/10.17504/protocols.io.zx4f7qw

Sze-Xian Lim¹

¹Duke University

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Sze-Xian Lim

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DOI: https://dx.doi.org/10.17504/protocols.io.zx4f7qw

**Protocol Citation:** Sze-Xian Lim 2019. Zymoclean™ Gel DNA Recovery. protocols.io

https://dx.doi.org/10.17504/protocols.io.zx4f7qw

**License:** This is an open access protocol distributed under the terms of the **Creative Commons Attribution License**, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

**Protocol status:** In development

We are still developing and optimizing this protocol
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**Created:** April 09, 2019

**Last Modified:** April 09, 2019

**Protocol Integer ID:** 22236

**Keywords:** dna

Troubleshooting
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1. Excise the DNA fragment from the agarose gel using a razor blade, scalpel or other device and transfer it into a 1.5 ml microcentrifuge tube.

2. Weight empty 1.5 ml microcentrifuge tube.

3. Weight tubes with agarose gel and subtract the weight of the empty tube.

4. Add 3 volumes of ADB to each volume of agarose excised from the gel (e.g. add 300 µL ADB to every 100 mg agarose gel).

5. Incubate at 37 °C minimum to 55 °C maximum for 00:05:00 minimum to 00:10:00 maximum until the gel slice is completely dissolved.

6. Transfer the melted agarose solution to a Zymo-Spin™ Column in a Collection Tube.

7. Centrifuge for 00:00:30 minimum to 00:01:00 maximum seconds.

8. Discard the flow-through.

9. Add 200 µL DNA Wash Buffer of DNA Wash Buffer to the column and centrifuge for 00:00:30.

10. Discard the flow-through.
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11 Repeat the wash step by adding ⏻ 200 µL DNA Wash Buffer of DNA Wash Buffer to the column and centrifuge for 🕒 00:00:30 .

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12 Add at least ⏻ 6 µL DNA Elution Buffer of DNA Elution Buffer directly to the column matrix.

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13 Place column into a 1.5 ml tube and centrifuge for 🕒 00:00:30 minimum to 🕒 00:01:00 maximum seconds to elute DNA.

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