# Multigram synthesis of an orthogonally-protected pentasaccharide for use as a glycan precursor in a Shigella flexneri 3a conjugate vaccine: application to a ready-for-conjugation decasaccharide

Johan Cornil, Zhaoyu Hu, Marion Bouchet, Laurence A Mulard

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Johan Cornil, Zhaoyu Hu, Marion Bouchet, Laurence A Mulard. Multigram synthesis of an orthogonally-protected pentasaccharide for use as a glycan precursor in a Shigella flexneri 3a conjugate vaccine: application to a ready-for-conjugation decasaccharide. Organic Chemistry Frontiers, 2021, 8 (22), pp.6279-6299. 10.1039/d1qo00761k. pasteur-03722329

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Cite this: *Org. Chem. Front.*, 2021, **8**, 6279

# Multigram synthesis of an orthogonally-protected pentasaccharide for use as a glycan precursor in a *Shigella flexneri* 3a conjugate vaccine: application to a ready-for-conjugation decasaccharide†

Johan Cornil,‡ Zhaoyu Hu,‡§ Marion Bouchet and Laurence A. Mulard ID*

The rapidly growing interest in carbohydrate-based bioactive molecules calls for strategies enabling the appropriate design and large-scale delivery of the glycan moiety. Herein, we describe the robust and high-yielding chemical synthesis of an orthogonally-protected pentasaccharide intended for use as a central building block in vaccine development against *Shigella flexneri* 3a. Elaborated from advanced crystalline intermediates and fine-tuned catalytic processes facilitating regio- and stereoselective conversions, a robust [2 + 3] strategy was designed, which avoided several tedious purifications and efficiently delivered multigram amounts of the target pentasaccharide. Conversion of this intermediate into a donor and a linker-equipped acceptor then merging into the frame of a [5 + 5] glycosylation step furnished a decasaccharide encompassing one trichloroacetamide moiety per repeat. Chemoselective develulination and subsequent Pd(OH)₂-mediated hydrogenolysis enabling concomitant hydrodechlorination and azide reduction gave the ready-for-conjugation dimer of the repeating unit of the O-antigen from *S. flexneri* 3a featuring the natural stoichiometric O-acetylation. The proof-of-concept was established, opening the way to larger *S. flexneri* 3a oligosaccharides and fine-tuned glycoconjugates.

Received 15th May 2021,
Accepted 15th July 2021

DOI: 10.1039/d1qo00761k

rsc.li/frontiers-organic

## Introduction

Understanding the importance of carbohydrates as mediators of biological processes has substantiated major advances in oligosaccharide synthesis to overcome limitations associated with isolates from natural sources.¹ Various strategies are being explored, including enzymatic, chemo-enzymatic and chemical routes. The latter feature the greatest versatility in providing access to natural, as well as non-natural oligosaccharides. Programmable, one-pot solution-phase and methods for solid-phase and HPLC-assisted automated strategies exemplify some of the major ongoing investigations to accelerate the chemical synthesis of complex glycans.²⁻⁵ Significant developments have facilitated the expedited synthesis of diverse well-defined oligosaccharides, including homopoly-

mers of increasing chain length,⁶⁻⁸ and the total synthesis of the largest chemically assembled polysaccharides to date,⁹⁻¹¹ paving the way to useful probes for further investigating poorly understood carbohydrate-mediated vital biological events. When considering large heteropolymers and highly branched targets, solution-phase iterative block synthesis has remained an attractive strategy.¹²⁻²⁰ In particular, the successful delivery of glycans featuring several repeats strongly relies on the identification of building blocks empowering iterative homologation with high and reproducible glycosylation yields, while also obeying regio- and stereoselectivity criteria in addition to qualifying for efficient full deprotection. Another significant challenge for relevant building block design stems from the need for a synthesis enabling the large-scale production of these essential intermediates to subsequently deliver usable amounts of the extended glycan targets.²¹,²² Herein, we tackle this relevant issue in the context of vaccine development.

Shigellae are Gram-negative bacteria and the cause of shigellosis, a major diarrheal disease responsible for a high burden, notably among children aged 1–5 years living in low- and middle-income settings.²³,²⁴ *Shigella* is on the WHO pathogen priority list. Epidemiological data, among which the increasing antimicrobial resistance observed among field isolates, call for the development of a multivalent *Shigella* vaccine.²³,²⁵ Toward this goal, conjugate vaccines based on the

Unité de Chimie des Biomolécules, Institut Pasteur, UMR3523 CNRS, 28 rue du Dr Roux, 75 724 Paris Cedex 15, France. E-mail: laurence.mulard@pasteur.fr

†Electronic supplementary information (ESI) available: Schemes S1 and S2, general procedures, detailed experimental procedures and analytical data for compounds S1–S3, 1–3, 5, 8, 12a, 12b, 13, 15–17, 22–25, copies of the ¹H and ¹³C NMR spectra for all new compounds. See DOI: 10.1039/d1qo00761k

‡These authors have contributed equally.

§Z.H.: Systems Biology Theme, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China

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bacterial polysaccharide antigens, or surrogates thereof, have been the subject of major interest.²⁶ As part of the ongoing developments, we have proposed the first synthetic glycan-protein conjugate vaccine candidate against endemic shigello-sis.²⁷ While many antibacterial glycovaccine candidates use haptens corresponding to one repeating unit of the homolo-gous natural polysaccharide antigens,²⁸ the selected glycocon-jugate prototype comprises a chemically synthesized pentade-casaccharide corresponding to a three-core repeating unit portion from the *Shigella flexneri* 2a O-antigen (O-Ag).²⁹ It was produced according to good manufacturing practice and was demonstrated to be safe and immunogenic in adult volunteers in the frame of a first-in-human clinical trial.³¹ These achieve-ments have provided strong support for serotype broadening. In this context, our efforts were aimed towards a vaccine candi-date against *S. flexneri* 3a (SF3a), another prevalent *Shigella* serotype for which a vaccine is in high demand.²⁴

The SF3a O-Ag is made up of a branched pentasaccharide repeat (E)AB~Ac~C~Ac~D (Fig. 1), featuring (1 → 2)-trans-linked L-rhamnoses (A, B, C) and an N-acetyl-D-glucosamine residue (D). Rhamnose A is 3-O-α-D-glucosylated (E). Acetylation at position 2~C~ is stoichiometric. In contrast, position 6~D~ is O-acetylated only to an extent of 40%.³⁰ Epitope mapping has revealed the immunodominant 2~C~O-acetyl (Ac) moiety and the importance of chain length for protective antibody reco-gnition.³² Molecular modeling simulations were supported by NMR analysis of O-Ag segments from 12 *S. flexneri* serotypes featuring the same backbone, among which those relevant to *S. flexneri* 2a and SF3a suggested similar backbone confor-mational behavior.³³ This study also revealed the dynamic be-havior of the end-chain α-D-glucopyranosyl residue (1 → 3)-linked to rhamnose A, differing from that predicted for glucose side-chains located on internal repeats.³³ Overall, con-vincing evidence supports the assumption that oligosacchar-ides achieving SF3a O-Ag functional mimicry encompass at least two repeating units. Otherwise, the role of the non-stoi-chiometric 6~D~O-acetylation remains undisclosed.

Aiming at establishing a lead hapten candidate for SF3a vaccination, we report a straightforward multi-step chemical synthesis of pentasaccharide 1¹⁷ as the lead common precursor to the (E)AB~Ac~CD and (E)AB~Ac~C~Ac~D modules, their combi-

<figure><img src="image_5.png" /><figcaption>**Fig. 1** Repeating unit of the SF3a O-Ag: (E)AB~Ac~C~Ac~D.³⁰</figcaption></figure>

nations and oligomers thereof, as found in the native SF3a O-Ag (Scheme 1). Going beyond our previous disclosures while aiming at scalability and robustness, the orthogonally-protected pentasaccharide building block was produced in several 10-gram amounts. Emphasis was placed on the following: (i) restraining the handling of toxic and poor user-friendly reagents, in particular by circumventing the notoriously questionable tin chemistry and by avoiding concerns related to hydrazine and its derivatives, especially when involved at an advanced stage of a multi-step synthesis; (ii) limiting the repeated use of low-abundant catalysts, despite their remarkable potential as exemplified by iridium-based compounds; (iii) reducing the number of demanding purification steps involving column chromatography by promoting crystalline intermediates and the fine-tuning of reaction parameters, while (iv) achieving high-yielding conversions fulfilling region- and stereoselectivity criteria. It is well-appreciated that concern for the latter increases when addressing glycosylation steps involved in large oligosaccharide blockwise synthesis.

Herein, significant inputs feature handy metal-catalyzed protecting group manipulation, advanced crystalline intermediates, fine-tuned 1,2-cis and block glycosylation steps, and a meaningful reduction of the number of columns for chromatography, the latter being known to qualify as a bottleneck when aiming at large-scale synthesis.²² Furthermore, the proof-of-concept is established since the potential of the

<figure><img src="image_6.png" /><figcaption>**Scheme 1** Pentasaccharide 1 and its retrosynthetic analysis. All: allyl; Lev: levulinoyl.</figcaption></figure>
selected pentasaccharide building block is demonstrated in the synthesis of a ready-for-conjugation linker-equipped deca-saccharide corresponding to a dimer of the repeating unit of the SF3a O-Ag.

## Results and discussion

Building block **1** was designed as an allyl glycoside, allowing easy conversion into a donor or an acceptor.¹⁷ It is 2c-O-acetylated as in the SF3a O-Ag. In contrast, the second site of natural O-acetylation was masked as a 4D,6D-O-benzylidene (Bzl) acetal, allowing for the chemoselective late-stage modification at OH-6D. Non-interfering hydroxyl groups are benzylated, and the site of elongation (OH-2A) features a levulinoyl ester, which fulfills the criteria for stability, anchimeric assistance and orthogonality, in particular to the 2c-acetate.³⁴ Relying on the imidate chemistry, pentasaccharide **1** is readily accessible from the known BAcCD and EA allyl glycosides, 2¹⁷ and 3,³⁵ respectively. These key intermediates are commercially available in bulk amounts. Substantiating our previous report,³⁶ diol 5 is routinely obtained in at least 90 g amounts in four steps and over 80% yield (Scheme 1). It performs as an exquisite common precursor to the known acceptor A/C (14) and donor B (13).³⁵

### Synthesis of the BCD trisaccharide 2

Going beyond the original tin-mediated regioselective benzyla-tion of 1,2-cis diols³⁷ and the inherent toxicity of tin reagents used in stoichiometric amounts, elegant procedures enabling the site-selective modification of carbohydrates have been developed.³⁸,³⁹ The recently reported iron(III)-based catalysts, Fe(dibm)₃, offering high regioselectivity, broad scope and high reactivity,⁴⁰ and its cheaper, although equally efficient, analog Fe(dipm)₃,⁴¹ attracted our attention (Scheme 2). Readily obtained from the inexpensive FeCl₃·6H₂O, these reagents are considered non-air sensitive, non-toxic and environmentally benign.⁴² Gratifyingly, the Fe(dibm)₃-promoted benzylation of diol 5 in the presence of base proceeded at 80 °C in aceto-nitrile to give the desired alcohol 7 (92%) together with its regioisomer (5%). Satisfactorily, Fe(dipm)₃ performed as well. The 19:1 regioselectivity compares nicely with the 87% yield achieved using tin chemistry.³⁵ Advantageously, purification is simpler. Next, instead of using a large excess of levulinic anhy-

<figure><img src="image_7.png" /><figcaption>**Scheme 2** Synthesis of hemiacetal **9** from diol **5**. (i) BnBr, Fe(dibm)₃ or Fe(dipm)₃ (5 mol%), K₂CO₃, MeCN, 80 °C, 92%; (ii) LevOH, EDC, DMAP, DCM, 90%. dibm: diisobutyrylmethane; dipm: dipivaloylmethane; LevOH: levulinic acid.</figcaption></figure>

dride prepared upfront, Steglich esterification of alcohol 7 gave levulinate **8**,³⁵ which was, in turn, deallylated into hemiacetal **9**.³⁵

As an attempt to avoid the previously adopted efficient, albeit expensive, [Ir(COD){PCH₃(C₆H₅)₂}]⁺PF₆⁻ catalyst and its necessary hydrogen-mediated activation (Table 1, entry 1),¹⁷ we have favoured the use of more earth-abundant metal catalysts, focusing primarily on well-explored palladium derivatives (Table 1) amid numerous possible reagents,⁴³⁻⁴⁵ to complete the anomeric deallylation step.⁴⁶ Unexpectedly, Pd(PPh₃)₄ used in combination with mild acids⁴⁷ led at best in partial conversion to propen-1-yl **10** (entries 2 and 3). Therefore, established protocols involving Pd(II) catalysts, which are generally more stable and less expensive than Pd(0) derivatives, were considered instead. Diverging from previous observations,⁴⁸ PdCl₂ in buffered AcOH/AcONa was low-yielding (entry 4). Although the phenomenon was barely reported, methyl glycoside **11** was repeatedly isolated when using PdCl₂ in methanol (entries 5 and 6), while the Wacker-type products⁴³,⁴⁵ **12a/12b** were formed in DMF (entries 7 and 8). We reasoned that changing DMF to a non-polar solvent used in combination with water as the proton source would prevent side-oxidation. Indeed, conversion to propen-1-yl **10** was slow, but oxidized **12a/12b** were not observed in DCM/H₂O (entry 9). Otherwise, changing DMF for THF led to low conversion (entry 10). Gratifyingly, heating rhamnoside **8** to 50 °C for 2 h in DCM/H₂O (3:1) containing PdCl₂ (4 mol%) allowed faster completion and provided hemiacetal **9** in quantitative yield post-iodine addition (entry 11). These yet unreported easy-to-handle conditions were adopted on a large scale (entries 12 and 13).

Remarkably, trichloroacetimidate **13** is easily obtained by reacting hemiacetal **9** and trichloroacetonitrile in the presence of a base,⁴⁹ and is now routinely prepared on the 40 g scale (92%) from alcohol 7 in three steps and no intermediate purification (Scheme 3). Donor **13** is stable for at least a month at -20 °C despite being isolated as a syrup.

The stepwise conversion of diol 5 into the BC donor **17** is a robust process (Scheme 3), reaching 69% over four steps on a

<table><caption>**Table 1** Pd-mediated isomerization of rhamnoside **8**</caption><thead><tr><th>Entry<sup>a</sup></th><th>Conditions</th><th>Products (yield)</th></tr></thead><tbody><tr><td>1<sup>b 49</sup></td><td>[Ir(COD){PCH<sub>3</sub>(C<sub>6</sub>H<sub>5</sub>)<sub>2</sub>}<sub>2</sub>]<sup>+</sup>PF<sub>6</sub><sup>-</sup></td><td>9 (93%)</td></tr><tr><td>2</td><td>Pd(PPh<sub>3</sub>)<sub>4</sub>, TsOH</td><td>8</td></tr><tr><td>3</td><td>Pd(PPh<sub>3</sub>)<sub>4</sub>, AcOH</td><td>10 (70%)</td></tr><tr><td>4</td><td>PdCl<sub>2</sub>, AcOH/AcONa</td><td>10 (58%)</td></tr><tr><td>5</td><td>PdCl<sub>2</sub>, MeOH</td><td>11</td></tr><tr><td>6</td><td>PdCl<sub>2</sub>, MeOH/THF</td><td>11</td></tr><tr><td>7</td><td>PdCl<sub>2</sub>, CuCl, DMF</td><td>12a/12b (85%)</td></tr><tr><td>8</td><td>PdCl<sub>2</sub>, DMF/H<sub>2</sub>O</td><td>12a/12b (74%)</td></tr><tr><td>9</td><td>PdCl<sub>2</sub>, DCM/H<sub>2</sub>O</td><td>10 (74%), 9</td></tr><tr><td>10</td><td>PdCl<sub>2</sub>, THF/H<sub>2</sub>O</td><td>8, 10</td></tr><tr><td>11<sup>b</sup></td><td>PdCl<sub>2</sub>, DCM/H<sub>2</sub>O, 2-4 h, 50 °C</td><td>9 (full conversion)</td></tr><tr><td>12<sup>b,c</sup></td><td>PdCl<sub>2</sub>, DCM/H<sub>2</sub>O, 2-4 h, 50 °C</td><td>9 (88%), 8 (12%)</td></tr><tr><td>13<sup>b,d</sup></td><td>PdCl<sub>2</sub>, DCM/H<sub>2</sub>O, 2-4 h, 50 °C</td><td>9 (crude)</td></tr></tbody></table>

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<footnote>a 60 mg scale and rt unless stated otherwise. b Post hydrolysis. c 25 g scale. d From alcohol 7 (30 g).</footnote>
Research Article

Organic Chemistry Frontiers

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<figure><img src="image_10.png" /><figcaption>**Scheme 3** Synthesis of donor **13** from alcohol **7** (top), and donor **17** from diol **5** (bottom). (i) LevOH, EDC, DMAP, DCM, 90%. (ii) PdCl₂, DCM/H₂O, 50 °C then I₂, THF/H₂O, 88% for **9** and 90% for **16**; (iii) Cl₃CCN, DBU, 1,2-DCE, 87% for **13** and 84% for **17**; (iv) LevOH, DCC, DMAP, DCM; (v) MeC(OMe)₃, PTSA·H₂O, MeCN, rt then 80% aq. AcOH, 0 °C; (vi) **13**, TMSOTf, 4 Å MS, toluene, -78 °C to rt, 93%; (vii) H₂-activated [Ir(COD)₂(PCH₃(C₆H₅)₂)⁺PF₆⁻](2-3 mol%), THF then I₂, THF/H₂O.</figcaption></figure>

5-10 g scale.¹⁷ Herein, this conversion was achieved without intermediate purification reaching an overall yield of 86%, which was proven reproducible upon scaling up. Donor 17 was isolated in a 30 g amount (84%) starting from 13 g of diol 5. Noteworthy features in doing so include the reaction of acceptor **14**, readily obtained from diol 5 as a 95:5 mixture of regioisomers, with a reduced excess of donor **13** (1.1 instead of 1.2

<figure><img src="image_11.png" /><figcaption>**Scheme 4** Synthesis of trisaccharide **2** from tetraacetate **4**. (i) a. Cl₃CC(O)Cl, pyridine, DCM; b. AlOH, TMSOTf, DCM; c. NaOMe, MeOH; d. PhCH(OMe)₂, CSA, MeCN, 88%.⁵⁰ (ii) **17**, TMSOTf, 4 Å MS, DCM, 0 °C to rt, 86% (45 g scale). (iii) Crude **17** from **15**, TMSOTf, 4 Å MS, DCM, 0 °C to rt, 97% (11 g scale). TCA: trichloroacetyl.</figcaption></figure>

equiv.) and the use of the newly established Pd(II)-mediated anomeric deallylation protocol without any yield loss, as demonstrated for the independent conversion of rhamnobio side **15** into trichloroacetimidate **17** (91%).

Otherwise, acceptor **18** (88%) was achieved from tetraacetate **4** in four steps as described.⁵⁰ In line with expectation,¹⁷ the TMSOTf-promoted [**18** + **17**] glycosylation proved to be highly efficient (Scheme 4). Crystalline BₐcCD **2** of acceptable purity for the next step was isolated in 97% yield from 11 g of crystalline **18** and a slight excess (1.15 equiv.) of crude **17** is obtained from disaccharide **15**.

### Synthesis of the EA donors **34** and **35**

The synthesis of disaccharide **3** was another opportunity for improvement (Scheme 5, Table 2). Originally, the essential 1,2-cis EA linkage was achieved from diol **5** as established in the late nineties⁵¹ to give alcohol **24** in 61% yield over three steps (entry 1).³⁶ Since then, a better understanding of the factors affecting stereoselectivity has guided several reports on strategies addressing the challenge of anomeric control during 1,2-cis glycosylation.⁵²,⁵³ Therefore, going beyond original achievements,³⁶ while considering scaling up, easy-to-implement alternatives were explored. Relying on the originally favored

<figure><img src="image_12.png" /><figcaption>**Scheme 5** Synthesis of disaccharides EA from diol **5** and tetrabenzyl glucosyl donors. (i) MeC(OMe)₃, PTSA·H₂O, MeCN, rt then 80% aq. AcOH, 0 °C.</figcaption></figure>

<figure><img src="image_13.png" /></figure>
<table><caption>**Table 2** Synthesis of disaccharide 23<sup>63</sup> and, in turn, the key intermediate 24 from diol 5 and donor 21, unless stated otherwise</caption><thead><tr><th>Entry</th><th>Conditions<sup>a</sup><br>Promotor (equiv.), solvent, temperature</th><th>Products<sup>b,c</sup> (α:β ratio, yield from 5)</th></tr></thead><tbody><tr><td>1<sup>36</sup></td><td>TMSOTf (0.02), toluene/DCM, -78 °C to rt</td><td>23 (85:15), 24 (61%)</td></tr><tr><td>2</td><td>TMSOTf (0.3), Et<sub>2</sub>O, -78 °C</td><td>23 (80:20, 60%)</td></tr><tr><td>3</td><td>TfOH (0.3), Et<sub>2</sub>O, -78 °C</td><td>23, 14, 26</td></tr><tr><td>4</td><td>Bi(OTf)<sub>3</sub> (0.3), Et<sub>2</sub>O, -78 °C</td><td>23 (85:15)</td></tr><tr><td>5</td><td>TMSOTf (0.07), Et<sub>2</sub>O, -105 °C</td><td>23, 14, 26</td></tr><tr><td>6</td><td>TMSOTf (0.3), toluene, -78 °C</td><td>23 (80:20), 26</td></tr><tr><td>7</td><td>TMSOTf (0.07), toluene, -78 °C</td><td>23 (85:15)</td></tr><tr><td>8</td><td>TfOH (1.0), DMF (20), DCM, -78 °C to rt</td><td>23 (90:10, 76%)</td></tr><tr><td>9<sup>d,f</sup></td><td>TfOH (1.0), DMF (20), DCM, -78 °C to rt</td><td>24 (88%)</td></tr><tr><td>10<sup>e,f</sup></td><td>TfOH (1.0), DMF (20), DCM, -78 °C to rt</td><td>24/25 (95:5, 81%)</td></tr><tr><td>11<sup>g</sup></td><td>TMSOTf (0.3), Et<sub>2</sub>O, -78 °C</td><td>23 (50:50, 73%)</td></tr><tr><td>12<sup>g</sup></td><td>TfOH (1.0), DMF (20), DCM, rt</td><td>23 (90:10)</td></tr><tr><td>13<sup>d,f,g</sup></td><td>TfOH (1.0), DMF (20), DCM, rt</td><td>24 (79%)</td></tr><tr><td>14<sup>h</sup></td><td>NIS/AgOTf, DCM, -20 °C</td><td>23 (65:35, 88%)</td></tr><tr><td>15<sup>i</sup></td><td>SnCl<sub>2</sub>/AgClO<sub>4</sub>, THF, -10 °C</td><td>23 (65:35, 55%), 6</td></tr></tbody></table>

<sup>a</sup> Reactions were run on 70–80 mg of diol 5, using 1.3 equivalents of donor 21 and 0.3 promotor equivalent, at -78 °C unless stated otherwise.

<sup>b</sup> α/β ratio based on NMR data of the crude. <sup>c</sup> Isolated yields for 23 and 24 are from diol 5, over two steps and over three steps, respectively. <sup>d</sup> 1.0 g scale. <sup>e</sup> 11 g scale. <sup>f</sup> Post transesterification. <sup>g</sup> Use of donor 22. <sup>h</sup> Use of donor 19. <sup>i</sup> Use of donor 20.

glucosyl donor 21<sup>54</sup> and reinforcing previous realizations, parameters such as the promotor, its amount, and the solvent (entries 2–7) were varied without any observed meaningful improvement. While avoiding the formation of the Chapman rearrangement product 26,<sup>55</sup> the use of the (N-phenyl)trifluoroacetimidate (PTFA) donor 22<sup>56</sup> resulted in the loss of α/β selectivity (entry 11). In agreement with former investigations, changing donor 21 for the corresponding known thiophenyl glycoside 19<sup>57</sup> and fluoride 20<sup>58</sup> met no success (entries 14 and 15).

Next, the possible remote anchimeric assistance of the protecting groups masking the [E + A] glycosylation outcome<sup>52</sup> was examined. In view of their orthogonal properties and easy access by means of the selective 6-O-debenzylation of hemiacetal 6 (Scheme 6),<sup>59</sup> glucosyl donors bearing a temporary 6-O-

acetyl ester or 6-O-tert-butyldiphenylsilyl ether (TBDPS), respectively, were considered. However, the enhanced α/β ratio expected from long-range 6-O-acyl-assistance using donors 28<sup>60</sup> and 29 or from a foreseeable steric hindrance-controlled α-glucosylation by means of the silylated analogs 31<sup>61</sup> and 32 was not observed in our hands providing the condensation products in at best a 7:3 α/β ratio (Scheme S2,† not described). Leaving aside promising albeit more demanding strategies involving specific protecting group manipulation,<sup>52</sup> we turned to investigate the potential of exogenous nucleophiles to control stereoselectivity<sup>62</sup> when solely considering the more readily available tetrabenzyl donors, and in particular imidates 21 and 22, as the simplest possible E precursors. We prioritized the DMF-modulated glycosylation strategy introduced by the Mong laboratory,<sup>64</sup> a highly attractive approach as valubly demonstrated by Codée and coworkers.<sup>65</sup> Satisfactorily, an improved α/β ratio was observed when rhamnoside 14 was reacted with trichloroacetimidate 21 in DCM containing DMF and stoichiometric TfOH (Table 2, entry 8).<sup>65</sup> This tendency was independent of the imidate donor (entry 12). Besides the further strengthening of the potential of DMF as an external modulator of glycosylation reactions, the observed enhanced stereoselectivity was compatible with the subsequent transesterification step, therefore facilitating isolation of the product of α-glucosylation as alcohol 24 (entries 9 and 13). To our utmost satisfaction, scaling up did not interfere with stereoselectivity (entry 10). Indeed, as a clear step forward to a robust high-yielding process, these conditions delivered 25 g of the glucosylation products 24 and 25 in an excellent 95:5 α/β ratio and 81% yield over three steps from diol 5 and trichloroacetimidate 21, both of which are easily accessible crystalline materials.

Whereas Steglich levulination at OH-2<sub>A</sub> of disaccharide 24 hardly reached completion,<sup>35</sup> full conversion into the key intermediate 3 was achieved when substituting DCC by the more

<figure><img src="image_14.png" /><figcaption>**Scheme 6** Synthesis of the 6-O-modified glucopyranosyl donors from hemiacetal 6. (i) TFA, Ac<sub>2</sub>O, 0 °C, (ii) NH<sub>2</sub>NH<sub>2</sub>H<sub>2</sub>O, AcOH, DMF, 87% over two steps; (iii) CCl<sub>3</sub>CN, K<sub>2</sub>CO<sub>3</sub>, DCM, 85% for 28, 78% for 31; (iv) PTFACl, K<sub>2</sub>CO<sub>3</sub> acetone, 90% for 29, 40% over 4 steps for 32; (v) MeONa, MeOH; (vi) TBDPSCI, imidazole, DMAP, DMF.</figcaption></figure>

<figure><img src="image_15.png" /></figure>
<figure><img src="image_16.png" /></figure>

<figure><img src="image_17.png" /><figcaption>**Scheme 7** Synthesis of donors EA from diol 5 and tetrabenzyl glucosyl donors 21 or 22. (i) $MeC(OMe)_3$, PTSA·H₂O, MeCN, rt then 80% aq. AcOH, 0 °C; (ii) TfOH, DMF, 4 Å MS, DCM, -78 °C to rt; (iii) MeONa, MeOH/DCM, from 21 (1.15 equiv.), 88% (over two steps) and from 22 (1.3 equiv.), 79% (over three steps); (iv) LevOH, EDC, DMAP, DCM, 90% (30 g scale); (v) $[Ir(COD)(PCH_3(C_6H_5)_2)]^+PF_6^−$, THF, then $I_2$, THF/H₂O, 85% (29 g scale); (vi) $CCl_3CN$, DBU, DCE, -5 °C, 93% (23 g scale), also from 24, 67%, (42 g scale, 3 steps) together with 25 (30%); (vii) $PdCl_2$, DCM/ H₂O then $I_2$, THF, 50 °C; (viii) PTFACl, $K_2CO_3$, acetone, 90% (34 g scale, 3 steps).</figcaption></figure>

reactive EDC (Scheme 7). This successful *in situ* activation of levulinic acid advantageously replaced the formerly adopted conditions.³⁵ Deallylation, whether conventional³⁵ or using the newly established aforementioned $PdCl_2$ protocol, delivered the known hemiacetal 33³⁵ quantitatively for direct conversion into imidates 34³⁵ and 35. Alternatively, the fully protected 3

<figure><img src="image_18.png" /><figcaption>**Scheme 8** Synthesis of pentasaccharide 1 from the crystalline 2 and EA imidate donors 34 and 35. (i) $NH_2NH_2·H_2O$, pyridine/AcOH (3:2), 92%; (ii) ethylenediamine, pyridine/AcOH (3:2), 74% (corrected yield: 85%); (iii) 34, TMSOTf, 4 Å MS, toluene, 94% (12 g)¹⁷ and 80% (27 g) from 2 (94% corrected based on isolated 36); (iv) 35, TMSOTf, 4 Å MS, toluene, 80% (24 g scale) from 2.</figcaption></figure>

was evolved into those same donors without intermediate purification. Scaling up this efficient three-step conversion provided trichloroacetimidate 34 in 67% yield in combination with hemiacetal 33 (30%), post chromatography. The recovery of a meaningful amount of 33 was attributable to donor hydrolysis on the column, suggesting that careful consideration be given to the purification step for large-scale development. Satisfactorily, the more stable PTFA donor 35 was isolated in 40 g amount in an excellent 90% yield over three steps.

## Synthesis of the EAB<sub>Ac</sub>CD pentasaccharide building block 1

Restraining the number of columns for chromatography, the two-step conversion of the fully protected 2 (~25 g) into pentasaccharide 1 employed the crude acceptor 36¹⁷ (Scheme 8). Satisfactorily, the independent use of donor 34 or 35 (1.15 equiv.) ensued a good 80% yield from the fully protected 2, or rather a 88–94% corrected yield based on recovered 36. Adding to the overall improved strategy of the (E)AB<sub>Ac</sub>CD building block (1), this compares favourably with original stepwise achievements.¹⁷ In particular, the proof of concept having been established, we are confident that additional fine-tuning on the two-step conversion on a large scale will contribute to further increasing the isolated yield of pentasaccharide 1. Toward this aim, we also envisioned alternatives to hydrazine acetate involving less toxic reagents for the selective delevulination at OH-2<sub>B</sub> of the 2<sub>C</sub>-O-acetyl B<sub>Ac</sub>CD precursor (2). While the former remains from far the method most frequently encountered, it is not without drawback. In particular, we previously observed the partial reduction of the olefinic bond of the allyl aglycon in pentasaccharide 1 concomitant to hydrazinolysis of the 2<sub>A</sub>-O-levulinoyl ester.¹⁷ Inspiration from earlier findings⁶⁶ encouraged the investigation of sulfa as a handy reagent. However, resulting at best in incomplete conversion, despite a prolonged reaction time (not described), neither the original conditions nor their modified version implemented in the context of oligonucleotide synthesis⁶⁷ fulfilled our expectations. Optimization was not attempted. Instead, the implementation of user-friendly conditions enabling the high-yielding delevulination of trisaccharide 2 took advantage of a previous report from R. Adamo's group.⁶⁸ Replacing hydrazine acetate by the more acceptable ethylenediamine provided alcohol 36 in a selective manner, suggesting that these conditions could be adopted in the future (Scheme 8).

## Synthesis of the spacer-equipped pentasaccharide 45 and decasaccharide 46

Having achieved the improved synthesis of the fully protected 1, the next step consisted of ensuring that this key building block fulfilled expectations when evolved into a donor and a linker-equipped acceptor, respectively. Toward this aim, pentasaccharide 1 was subjected to conventional deallylation into hemiacetal 38, which was in turn converted into the corresponding PFTA donor 39 in high yield (Scheme 9). Running the two steps without any intermediate purification also resulted in an efficient conversion, repeatedly reaching over 85% yield on a multigram scale.
<figure><img src="image_19.png" /><figcaption>**Scheme 9** Synthesis of decasaccharide 40 from the pentasaccharide building block 1. (i) Ethylenediamine, pyridine/AcOH (3 : 2), 89%; (ii) [Ir(COD)(PCH₃(C₆H₅)₂)₂]⁺PF₆⁻, THF, then I₂, THF/H₂O, 92%; (iii) PTFACl, K₂CO₃, acetone, 0 °C, 93%.</figcaption></figure>

<table><caption>**Table 3** [5 + 5] Glycosylation to reach decasaccharide 40</caption><thead><tr><th rowspan="2">Entry</th><th colspan="3">Conditions<sup>a</sup></th><th rowspan="2">Product (yield)<br/>Temperature</th></tr><tr><th>Acceptor</th><th>39<br/>equiv.</th><th>Solvent</th></tr></thead><tbody><tr><td>1</td><td>37</td><td>1.3</td><td>DCM</td><td>-40 °C<br/>40 (90%)</td></tr><tr><td>2</td><td>37</td><td>1.3</td><td>DCM</td><td>rt<br/>40 (81%)</td></tr><tr><td>3</td><td>37</td><td>1.3</td><td>DCM</td><td>0 °C<br/>40 (86%)</td></tr><tr><td>4</td><td>37</td><td>1.1</td><td>DCM</td><td>-78 °C<br/>40 (83%), 37</td></tr><tr><td>5</td><td>37</td><td>1.1</td><td>DCM</td><td>-40 °C<br/>40 (85%), 37<br/>(10%)</td></tr><tr><td>6</td><td>37</td><td>1.2</td><td>DCM</td><td>-40 °C<br/>40 (87%), 37</td></tr><tr><td>7</td><td>37</td><td>1.3</td><td>Toluene</td><td>-40 °C<br/>40 (91%)</td></tr></tbody></table>

Alcohol 37 was used as a model acceptor for the [5 + 5] glycosylation envisioned next (Table 3). With the promising outcome of the ethylenediamine-mediated delevulination of

the BCD trisaccharide 2, it was isolated in an excellent 89% yield upon heating the fully protected **1** in the presence of excess ethylenediamine (Scheme 9). The [39 + 37] coupling proved to be high-yielding in all the conditions that were tested (Table 3). However, tendencies were revealed. In particular, some unconsumed acceptor was always observed when the 39:37 ratio was below 1.3 (entries 4–6). Glycosylation proceeded in a large range of temperature to give decasaccharide **40** in over 80% yield, but formation of an unidentified side-product was repeatedly observed at temperatures higher than –40 °C (entries 1–3). Replacing DCM with toluene while keeping the temperature at –40 °C and using 1.3 equivalents of donor had no obvious influence (entries 1 and 7). Having identified high-yielding glycosylation conditions, we turned to the synthesis of the linker-equipped decasaccharide **46**.

Glycosylation of donor **39** with 2-azidoethanol was achieved in toluene at –40 °C to give the β-linked azidoethyl glycoside **41**, which was isolated in a good 78% yield (Scheme 10). Hydrazinolysis of the 2<sub>A</sub>-O-levulinoyl ester provided acceptor

<figure><img src="image_20.png" /><figcaption>**Scheme 10** Synthesis of decasaccharide 46 from the pentasaccharide building block 1. (i) [Ir(COD)(PCH₃(C₆H₅)₂)₂]⁺PF₆⁻, THF, then I₂, THF/H₂O; (ii) PTFACl, K₂CO₃, acetone, 0 °C, 88% (over 2 steps); (iii) 2-azidoethanol, TMSOTf, toluene, –40 °C, 78%; (iv) NH₂NH₂·H₂O, pyridine/AcOH (3 : 2), 91% for **42** and 92% for **44**; (v) **39** (1.3 equiv.), 4 Å MS, toluene, –40 °C, 88%; (vi) Pd(OH)₂, H₂, tBuOH/DCM/H₂O, 70% for **45** from **42**, 52% for **46** from **44**.</figcaption></figure>

<figure><img src="image_21.png" /></figure>

<footnote><sup>a</sup> Reactions were run on 100 mg of acceptor 37 using TMSOTf (0.2 equiv.) as promotor.</footnote>
42 in a yield equivalent to that obtained for the corresponding allyl glycoside 37.¹⁷ In support of the selection of azidoethyl glycoside 42 as precursor to the larger linker-equipped SF3a oligosaccharides, its full deprotection promoted by Pd(OH)₂ in an hydrogen atmosphere was uneventful, permitting the smooth concomitant hydrogenolysis and reduction of all protecting groups in place, to give the expected aminoethyl penta-saccharide 45 in a satisfactory 70% yield post-RP-HPLC chromatography.

Interestingly, transferring the most promising [5 + 5] glycosylation conditions to the 2-azidoethyl-equipped acceptor revealed that the [39 + 37] glycosylation was somewhat sensitive to both solvent and temperature (not described). In agreement with original findings,¹⁷ running the condensation in non-polar toluene at -40 °C was identified as the best condition for providing decasaccharide 43 in a reproducible 90% average yield (Scheme 10). Subsequent delevulination gave alcohol 44, which was next subjected to a one-step full deprotection. While enabling the concomitant cleavage of the two 4D,6D-O-benzylidene acetals and 16 benzyl ethers in addition to the simultaneous reduction of the two 2D-trichloroacetamides and azide moiety, the Pd(OH)₂-catalyzed hydrogenation/hydrogenolysis of the azidoethyl glycoside 44 in tBuOH/DCM/H₂O into the aminoethyl decasaccharide 46 was more demanding than that of its counterpart 42 into penta-saccharide 45. The use of a higher Pd(OH)₂ amount combined with a longer reaction time at ambient temperature and pressure furnished the conjugation-ready 46 in a good 52% yield post-RP-HPLC (Scheme 10). Nevertheless, the observed drop in the yield of the two O-Ag repeating unit segment 46 versus the one repeating unit oligosaccharide 45 suggested that improvement might be needed for the full deprotection of larger oligomers featuring an aminoalkyl aglycon and a higher number of trichloroacetamide groups.

## Conclusions

This study was aimed at achieving a robust process to enable the large-scale synthesis of penta-saccharide 1, and demonstrating that this orthogonally-protected building block could serve as a suitable precursor to a donor and an acceptor, whose combination would provide ready-for-conjugation oligosaccharides for use in the development of a synthetic carbohydrate-based conjugate vaccine candidate against SF3a. A robust and convenient 26-step synthesis, featuring four crystallizations and only nine columns for chromatography – mother liquors included – of penta-saccharide 1 from crystalline 1,3,4-6-tetra-O-acetyl-D-glucosamine, L-rhamnose and tetrabenzyl-D-glucose is described. The upgraded synthesis combines several independent step-specific improvements involving greener, less demanding, more stereoselective and user-friendly protocols, also promoting crystalline intermediates and multigram scale validation. Notably, relevant improvements of interest in a broader context include the implementation of the easy-to-handle PdCl₂ in DCM/H₂O for high-yielding anomeric deallyla-

tion and catalytic Fe(dipm)₃ for the 3-O-etherification step of diol 5. Paving the way to further scale up and vaccine development against SF3a, the (E)ABₐcCD penta-saccharide 1 was readily delivered in amounts over 30 g and subsequently converted into the linker-equipped hapten 45 by means of acceptor 42. Alternatively, the fully protected 1 was efficiently transformed into donor 39. Lastly, the proof-of-concept for building block selection enabling a robust [5 + 5] chain elongation strategy from penta-saccharide 1 was successfully demonstrated by delivering the ready-for-conjugation decasaccharide 46, which corresponds to a two-repeating-unit segment of the SF3a O-Ag. Aminoethyl glycosides 45 and 46 and larger SF3a O-Ag segments are ideal substrates for use as components of well-defined glycos conjugates for in vivo study, which represents the next aim.

## Experimental

### Iron(III) dipivaloylmethane (Fe(dipm)₃)⁴¹,⁴²

To a biphasic mixture of 2,2,6,6-tetramethyl-heptane-3,5-dione (10.2 g, 55.5 mmol, 3.0 equiv.) and NaOAc (4.6 g, 55.5 mmol, 3.0 equiv.) in EtOH/H₂O (1:1, 140 mL) was added FeCl₃·6H₂O (5.0 g, 18.5 mmol). A red slurry was formed and the mixture was heated at 60 °C for 2 h. The reaction was cooled down to rt then to 0 °C for 15 min. Filtration gave an orange powder that was washed with water and crystallized using EtOH/H₂O (90:10, 70 mL). After cooling to 0 °C, crystals were filtered and rinsed using -78 °C cooled EtOH/H₂O (90:10), furnishing Fe(dipm)₃ as a red solid (10.9 g, 97%).

### Allyl 3,4-di-O-benzyl-α-L-rhamnopyranoside (7)⁶⁹

**Route 1.** To a solution of diol 5 (5.0 g, 17 mmol, 1.0 equiv.) in MeCN (150 mL) and benzyl bromide (2.22 mL, 19 mmol, 1.1 equiv.) K₂CO₃ (3.52 g, 25.5 mmol, 1.5 equiv.) and Fe(dibm)₃ (440 mg, 0.85 mmol, 5 mol%) were added at rt. The reaction mixture was stirred for 3 h at 80 °C. Additional benzyl bromide (1.5 equiv.) and Fe(dibm)₃ (2 mol%) were added and the mixture was stirred for an additional 24 h. A TLC control (tol/ EtOAc, 80:20) indicated the total conversion of diol 5 into less polar products. Solids were filtered over a pad of Celite® and washed generously with DCM. Purification by flash column chromatography (tol/EtOAc, 100:0 to 90:10 to 80:20) gave the known 2-O-benzyl isomer⁷⁰ as a yellow oil (350 mg, 5%) along with the desired 7 (6.0 g, 92%). The former had R<sub>f</sub> = 0.75 (cHex/EtOAc, 70:30). ¹H NMR (400 MHz, CDCl₃) δ 7.47-7.32 (m, 10H, H<sub>Ar</sub>), 5.94 (m, 1H, CH=CH<sub>2</sub>), 5.34 (dq, *J* = 17.2, 1.5 Hz, 1H, CH=CH<sub>2</sub>), 5.24 (m, 1H, CH=CH<sub>2</sub>), 4.98 (d, *J* = 11.2 Hz, 1H, H<sub>Bn</sub>), 4.94 (d, *J* = 1.5 Hz, 1H, H-1), 4.80 (d, *J* = 11.8 Hz, 1H, H<sub>Bn</sub>), 4.73 (d, *J* = 11.2 Hz, 1H, H<sub>Bn</sub>), 4.68 (d, *J* = 11.8 Hz, 1H, H<sub>Bn</sub>), 4.22 (m, 1H, CH<sub>2</sub>All), 4.08 (dd<sub>app</sub>, *J* = 9.0, 3.8 Hz, 1H, H-3), 4.02 (m, 1H, CH<sub>2</sub>All), 3.83 (dd, *J* = 3.8, 1.5 Hz 1H, H-2), 3.80 (dq, *J* = 9.4, 6.3 Hz, 1H, H-5), 3.33 (t<sub>app</sub>, *J* = 9.2 Hz, 1H, H-4), 2.53 (brs, 1H, OH), 1.42 (d, *J* = 6.3 Hz, 3H, H-6). ¹³C NMR (100 MHz, CDCl₃) δ 138.7 (C<sub>Ar</sub>), 137.9 (C<sub>Ar</sub>), 133.9 (CH=CH<sub>2</sub>), 128.6-127.7 (10CH<sub>Ar</sub>), 117.2 (CH=CH<sub>2</sub>), 96.3 (C-1), 82.4 (C-4), 78.8 (C-2), 75.1 (CH<sub>2</sub>Bn), 73.1 (CH<sub>2</sub>Bn), 71.8 (C-3),
67.8 (CH₂All), 67.4 (C-5), 18.1 (C-6). HRMS (ESI⁺): *m/z* 407.1865 (calcd for C₂₃H₂₈O₅Na [M + Na]⁺: *m/z* 407.1820).

**Route 2.** To a solution of diol 5 (400 mg, 1.36 mmol, 1.0 equiv.) in MeCN (7 mL) and benzyl bromide (0.19 mL, 1.63 mmol, 1.2 equiv.) were added K₂CO₃ (282 mg, 2.04 mmol, 1.5 equiv.) and Fe(dipm)₃ (440 mg, 0.85 mmol, 5 mol%) at rt. The reaction mixture was stirred for 4 h at 80 °C. Additional benzyl bromide (1.5 equiv.) and iron catalyst (2 mol%) were added and the mixture was stirred for an additional 10 h. A TLC control (tol/EtOAc, 80:20) indicated the total conversion of diol 5 into less polar products. Solids were filtered over a pad of Celite® and washed generously with DCM. Purification by flash column chromatography (tol/EtOAc, 100:0 to 90:10 to 80:20) gave the known 3-O-benzyl isomer as a yellow oil (480 mg, 92%). The target 7 had R<sub>f</sub> = 0.55 (cHex/EtOAc, 70:30). ¹H NMR (400 MHz, CDCl₃) δ 7.46-7.30 (m, 10H, H<sub>Ar</sub>), 5.91 (dddd, *J* = 17.2, 10.4, 6.1, 5.1 Hz, 1H, CH=CH₂), 5.30 (dq, *J* = 17.2, 1.6 Hz, 1H, CH=CH₂), 5.21 (dq, *J* = 10.4, 1.4 Hz, 1H, CH=CH₂), 4.92 (d, *J* = 10.9 Hz, 1H, H<sub>Bn</sub>), 4.88 (d, *J* = 1.7 Hz, 1H, H-1), 4.72 (s, 2H, H<sub>Bn</sub>), 4.67 (d, *J* = 10.9 Hz, 1H, H<sub>Bn</sub>), 4.18 (ddt<sub>app</sub>, *J* = 12.9, 5.1, 1.5 Hz, 1H, CH₂All), 4.09 (dd, *J* = 3.4, 1.8 Hz, 1H, H-2), 4.00 (ddt<sub>app</sub>, *J* = 13.0, 6.1, 1.4 Hz, 1H, CH₂All), 3.90 (dd, *J* = 9.1, 3.4 Hz, 1H, H-3), 3.79 (dq, *J* = 9.5, 6.2 Hz, 1H, H-5), 3.49 (t<sub>app</sub>, *J* = 9.3 Hz, 1H, H-4), 2.51 (brs, 1H, OH), 1.34 (d, *J* = 6.3 Hz, 3H, H-6). ¹³C NMR (100 MHz, CDCl₃) δ 138.4 (C<sub>Ar</sub>), 138.0 (C<sub>Ar</sub>), 133.8 (CH=CH₂), 128.5-127.7 (10CH<sub>Ar</sub>), 117.4 (CH=CH₂), 98.2 (C-1, *J*<sub>C,H</sub> = 168.7 Hz), 80.1 (C-3), 80.0 (C-4), 75.4 (CH₂Bn), 72.1 (CH₂Bn), 68.6 (C-2), 67.9 (CH₂All), 67.4 (C-5), 17.9 (C-6). HRMS (ESI⁺): *m/z* 407.1809 (calcd for C₂₃H₂₈O₅Na [M + Na]⁺: *m/z* 407.1820).

### 3,4-Di-O-benzyl-2-O-levulinoyl-α-β-L-rhamnopyranose (9)³⁵

PdCl₂ (769 mg, 2.6 mmol, 0.05 equiv., 60% purity) was added to a solution of allyl glycoside **8** (25.1 g, 52.0 mmol) in DCM/H₂O (3:1, 260 mL). After stirring for 4 h at 50 °C, a TLC control (tol/EtOAc, 80:20) showed the presence of a major less polar product in addition to some remaining **8**. Nevertheless, the reaction mixture was allowed to cool to rt and I₂ (26.4 g, 104.1 mmol, 2.0 equiv.) was added. After 40 min, a TLC control (tol/EtOAc, 80:20) showed the formation of two more polar products. Saturated aq. Na₂S₂O₃ was added and the biphasic solution was filtered on a bed of Celite® and cotton. The two layers were separated and the aq. phase was extracted twice with DCM. The combined organic layers were washed with sat. aq. NaHCO₃, water and brine, then dried on Na₂SO₄. Solids were filtered and volatiles were evaporated under reduced pressure. The crude residue was purified by flash chromatography (tol/EtOAc 90:10 then 70:30) to give, in order of elution, the unreacted **8** (3.08 g, 12%) as a brownish oil and the known hemiacetal **9** (20.19 g, α/β 85:15, 88%) as a light yellow oil. The obtained α anomer had R<sub>f</sub> = 0.25 (tol/EtOAc, 70:30). ¹H NMR (400 MHz, CDCl₃) δ 7.37-7.28 (m, 10H, H<sub>Ar</sub>), 5.40 (m, 1H, H-2), 5.13 (m, 1H, H-1), 4.95 (d, *J* = 10.9 Hz, 1H, H<sub>Bn</sub>), 4.75-4.51 (m, 2H, H<sub>Bn</sub>), 4.20 (d, *J* = 10.7 Hz, 1H, H<sub>Bn</sub>), 4.03-3.98 (m, 2H, H-3, H-5), 3.43 (t<sub>app</sub>, *J* = 9.4 Hz, 1H, H-4), 2.73 (m, 4H, CH₂Lev), 2.18 (s, 3H, CH₃Lev), 1.33 (d, *J* =

6.2 Hz, 3H, H-6). ¹³C NMR (100 MHz, CDCl₃) δ 206.8 (CO<sub>Lev</sub>), 172.5 (CO₂Lev), 138.9 (C<sub>Ar</sub>), 138.0 (C<sub>Ar</sub>), 128.8-128.0 (10CH<sub>Ar</sub>), 92.7 (C-1, *J*<sub>C,H</sub> = 170.3 Hz), 80.5 (C-4), 77.9 (C-3), 75.7 (CH₂Bn), 72.0 (CH₂Bn), 70.0 (C-2), 68.1 (C-5), 38.5 (CH₂Lev), 30.1 (CH₃Lev), 28.6 (CH₂Lev), 18.4 (C-6). HRMS (ESI⁺): *m/z* 465.1876 (calcd for C₂₅H₃₀O₇Na [M + Na]⁺: *m/z* 465.1889).

### Prop-1-enyl 3,4-di-O-benzyl-2-O-levulinoyl-α-L-rhamnopyranoside (10)

An analytical sample of the product of allyl isomerization from the fully protected **8** had R<sub>f</sub> = 0.7 (tol/EtOAc 70:30). ¹H NMR (400 MHz, CDCl₃) δ 7.44-7.30 (m, 10H, H<sub>Ar</sub>), 6.18 (dq, *J* = 12.3 Hz, 1.8 Hz, 0.2H, CH=CH-CH₃trans), 6.13 (dq, *J* = 6.2 Hz, 1.7 Hz, 0.8H, CH=CH-CH₃cis), 5.49 (dd, *J* = 3.3 Hz, 1.9 Hz, 0.8H, H-2cis), 5.45 (dd, *J* = 3.3 Hz, 1.9 Hz, 0.8H, H-2trans), 5.14 (dq, *J* = 12.1 Hz, 6.8 Hz, 0.2H, CH=CH-CH₃trans), 5.01-4.94 (m, 2H, H-1cis, H-1trans, H<sub>Bn,cis</sub>, H<sub>Bn,trans</sub>), 4.75 (d, *J* = 11.0 Hz, 0.8H, H<sub>Bn,cis</sub>), 4.73 (d, *J* = 10.4 Hz, 0.2H, H<sub>Bn,trans</sub>), 4.67 (d, *J* = 11.0 Hz, 0.8H, H<sub>Bn,cis</sub>), 4.67 (d, *J* = 11.0 Hz, 0.2H, H<sub>Bn,trans</sub>), 4.65-4.55 (m, 1.8H, CH=CH-CH₃cis, H<sub>Bn,cis</sub>, H<sub>Bn,trans</sub>), 4.02 (dd, *J* = 9.4 Hz, 3.4 Hz, 0.8H, H-3cis), 4.01 (dd, *J* = 9.1 Hz, 3.4 Hz, 0.2H, H-3trans), 3.85-3.76 (m, 1H, H-5cis, H-5trans), 3.48 (t, *J* = 9.5 Hz, 0.8H, H-4cis), 3.46 (t, *J* = 9.3 Hz, 0.2H, H-4trans), 2.81-2.70 (m, 4H, 2CH₂Lev), 2.19 (s, 2.4H, CH₃Lev,cis), 2.19 (s, 0.6H, CH₃Lev,trans), 1.60 (dd, *J* = 7.1 Hz, 1.7 Hz, 2.4H, CH=CH-CH₃cis), 1.59 (dd, *J* = 5.4 Hz, 1.7 Hz, 0.6H, CH=CH-CH₃trans), 1.36 (d, *J* = 6.4 Hz, 3H, H-6cis, H-6trans). ¹³C NMR (100 MHz, CDCl₃) δ 206.2 (CO<sub>Lev</sub>), 172.0 (CO₂Lev), 142.2 (CH=CH-CH₃trans), 141.1 (CH=CH-CH₃cis), 138.5-137.0 (2C<sub>Ar,cis</sub>, 2C<sub>Ar,trans</sub>), 128.4-126.9 (10CH<sub>ArBn,cis</sub>, 10CH<sub>ArBn,trans</sub>), 104.9 (CH=CH-CH₃trans), 104.2 (CH=CH-CH₃cis), 97.4 (C-1cis, *J*<sub>C,H</sub> = 173.0 Hz), 96.9 (C-1trans, *J*<sub>C,H</sub> = 173.0 Hz), 79.9 (C-4trans), 79.8 (C-4cis), 77.8 (C-3trans), 77.7 (C-3cis), 75.5 (CH₂Bn,cis), 75.4 (CH₂Bn,trans), 71.8 (CH₂Bn,cis), 71.7 (CH₂Bn,trans), 68.8 (C-2cis), 68.6 (C-2trans), 68.2 (C-5cis), 68.0 (C-5trans), 38.0 (CH₂Lev), 29.8 (CH₃Lev), 28.2 (CH₂Lev), 18.0 (C-6), 12.4 (CH=CH-CH₃trans), 9.3 (CH=CH-CH₃cis).

### 3,4-Di-O-benzyl-2-O-levulinoyl-α-L-rhamnopyranosyl trichloroacetimidate (13)³⁵

**Route 1.** To a solution of alcohol 7 (30.0 g, 78 mmol) in DCM (350 mL) levulinic acid (18.1 g, 156 mmol, 2.0 equiv.), DCC (20.9 g, 101 mmol, 1.3 equiv.) and DMAP (3.2 g, 16 mmol, 0.2 equiv.) were added successively. The mixture was stirred for two days at rt. TLC (DCM/EtOAc, 90:10) showed that the starting material had been converted to a less polar product. DCU was filtered by passing through a pad of Celite®, and the solids were washed extensively with DCM. The organic layer was washed with water, sat. aq. NaHCO₃, sat. aq. CuSO₄, then twice with water and finally with brine. The organic layer was dried over Na₂SO₄, filtered, and concentrated under reduced pressure. PdCl₂ (920 mg, 3.0 mmol, 0.04 equiv., 60% purity) was added to a solution of crude **8** in DCM/H₂O (3:1, 600 mL). The mixture was stirred for 3 h at 50 °C. TLC (cHex/EtOAc, 80:20) showed the conversion of the fully protected **8** into a less polar product. After cooling the solution to 0 °C, a
<figure><img src="image_22.png" /></figure>

solution of iodine (19.8 g, 156 mmol, 2.0 equiv.) in THF (140 mL) was added slowly and the mixture was stirred at rt for 2.5 h. TLC (cHex/EtOAc, 70:30) showed the complete disappearance of the intermediate and the presence of more polar products. Excess iodine was destroyed by adding a solution of sat. aq. Na₂S₂O₃. The biphasic mixture was filtered on cotton and the organic phase was washed with sat. aq. NaHCO₃, water and brine. The organic phase was dried over Na₂SO₄, filtered, and concentrated under reduced pressure. To a solution of the crude hemiacetal **9** in 1,2-DCE (260 mL) stirred under Ar at 0 °C, trichloroacetonitrile (39.1 mL, 390 mmol, 5.0 equiv.) and DBU (3.3 mL, 21.8 mmol, 0.28 equiv.) were added dropwise. The brown mixture was stirred at 0 °C for 3 h. TLC (cHex/EtOAc, 70:30 + 1% Et₃N) showed the conversion of the intermediate **9** into less polar products. After incomplete concentration under reduced pressure, the mixture was purified by column chromatography on neutralized silica gel (cHex/EtOAc, 100:0 to 60:40 + 1% Et₃N) to give the known donor **13** (42.6 g, 92%) as a yellow syrup along with recovered hemiacetal **9** (1.8 g, 5%).

**(3,4-Di-O-benzyl-2-O-levulinoyl-α-L-rhamnopyranosyl)-(1 → 3)-2-O-acetyl-4-O-benzyl-α/β-L-rhamnopyranosyl trichloroacetimidate (17)⁴⁹**

**Route 1.** To a solution of disaccharide **15** (43.7 g, 57.4 mmol) in DCM/H₂O (3:1, 575 mL) was added PdCl₂ (850 mg, 2.87 mmol, 0.05 equiv., 60% purity). The mixture was stirred for 3 h at 50 °C. TLC (tol/EtOAc, 80:20) showed the disappearance of the starting **15** and the presence of a less polar product. Iodine (14.6 g, 115 mmol, 2.0 equiv.) in THF (100 mL) was added slowly at 0 °C and the mixture was stirred at rt for 2.5 h. TLC (tol/EtOAc, 70:30) showed the complete disappearance of the intermediate and the presence of a single more polar product. Excess iodine was destroyed by adding sat. aq. Na₂S₂O₃. The biphasic mixture was filtered on cotton and the two layers were separated. The organic phase was washed with sat. aq. NaHCO₃, water and brine, dried over Na₂SO₄, filtered, and concentrated under reduced pressure. Trichloroacetonitrile (29 mL, 287 mmol, 5.0 equiv.) and DBU (2.5 mL, 16.1 mmol, 0.28 equiv.) were added dropwise to the obtained crude hemiacetal in anhyd. 1,2-DCE (290 mL) under Ar at 0 °C. After stirring at 0 °C for 3 h, TLC (tol/EtOAc 80:20, +1% Et₃N) showed the absence of hemiacetal **16** and the presence of less polar products. The solution was concentrated to a minimum of solvent and purified by column chromatography on neutralized silica gel (tol/EtOAc 100:0 to 80:20, +1% Et₃N). Donor **17** (45.0 g, 91%) was isolated as an orange syrup along with recovered **16** (3.05 g, 7%).

**Route 2.** Trimethyl orthoacetate (10.9 mL, 85.5 mmol, 1.9 equiv.) and monohydrated PTSA (128 mg, 0.68 mmol, 0.015 equiv.) were added to diol 5 (13.2 g, 45.0 mmol, 1.0 equiv.) in anhyd. MeCN (26 mL) at rt. After stirring at rt for 1.5 h, 80% aq. AcOH (26.5 mL) was added at 0 °C and the mixture was stirred at this temperature for 30 min, then at rt for 1 h. TLC (tol/EtOAc, 80:20) showed orthoester consumption and the presence of more polar products. DCM was added along with

water and the two layers were separated. The aq. phase was extracted with DCM and the combined organic layers were washed successively with sat. aq. NaHCO₃ and brine, dried over Na₂SO₄, filtered and evaporated under reduced pressure to give the crude acceptor **14**. TMSOTf (2.33 mL, 13.9 mmol, 0.25 equiv.) was slowly added dropwise to a solution of the latter (45.0 mmol, 1.0 equiv.) and trichloroacetimidate **13** (27.8 g, 47.3 mmol, 1.05 equiv.) in toluene (540 mL) containing activated 4 Å MS (5.0 g) at -78 °C. After stirring for 45 min at -78 °C, TLC (tol/EtOAc, 80:20) indicated acceptor consumption and the presence of a major less polar product. Et₃N (3 mL) was added, the mixture was filtered over a pad of Celite® (DCM) and concentrated to dryness. Filtration over a pad of silica gel (tol/EtOAc, 100:0 to 85:15), evaporation and precipitation of TCA salts by the addition of cold toluene gave crude disaccharide **15** as an orange oil. To a solution of the latter in DCM/H₂O (3:1, 430 mL) was added PdCl₂ (635 mg, 2.15 mmol, 0.05 equiv., 60% purity). The mixture was stirred for 3 h at 50 °C. TLC (tol/EtOAc, 80:20) revealed a single less polar product. After cooling the mixture to 0 °C, a solution of iodine (10.9 g, 86.0 mmol, 2.0 equiv.) in THF (60 mL) was added slowly and the mixture was stirred at rt for 2.5 h. TLC (tol/EtOAc, 80:20) indicated the conversion of the intermediate into a more polar product. Excess iodine was destroyed by adding a solution of sat. aq. Na₂S₂O₃. The mixture was filtered on cotton, and the two layers were separated. The organic phase was washed with sat. aq. NaHCO₃, water and brine, dried on Na₂SO₄, filtered, and concentrated under reduced pressure. To a solution of crude **16** in anhyd. 1,2-DCE (215 mL) under an inert atmosphere at 0 °C, trichloroacetonitrile (21.6 mL, 215 mmol, 5.0 equiv.) and DBU (1.84 mL, 12.0 mmol, 0.28 equiv.) were added dropwise. After stirring at 0 °C for 1 h and at rt for 15 h, TLC (cHex/EtOAc 70:30, +1% Et₃N) showed the conversion of hemiacetal **16** into less polar products. The solution was concentrated to a minimum of solvent and purified by flash chromatography on neutralized silica gel (cHex/EtOAc 100:0 to 80:20, +1% Et₃N) to give trichloroacetimidate **17** (31.5 g, 84% over 4 steps) as an orange oil.

**Allyl (2,3,4,6-tetra-O-benzyl-α-D-glucopyranosyl)-(1 → 3)-4-O-benzyl-α-L-rhamnopyranoside (24)⁵¹**

**Route 1 (1 g scale).** Trimethyl orthoacetate (820 µL, 6.45 mmol, 1.9 equiv.) and PTSA monohydrate (10 mg, 0.05 mmol, 0.015 equiv.) were added to diol 5 (1.0 g, 3.4 mmol, 1.0 equiv.) in anhyd. MeCN (2.3 mL) at rt. After stirring at rt for 1 h, 80% aq. AcOH (2.3 mL) was added at 0 °C and the mixture was stirred at this temperature for 30 min. TLC (tol/EtOAc, 70:30) showed total consumption of the intermediate orthoester. DCM was added along with water and the two layers were separated. The aq. phase was extracted with DCM and the combined organic phases were washed successively with sat. aq. NaHCO₃ and brine, dried over Na₂SO₄, filtered and concentrated to dryness to give crude acceptor **14**. TfOH (300 µL, 3.4 mmol, 1.0 equiv.) was slowly added to a solution of the latter and trichloroacetimidate⁷¹ 21 (2.68 g, 3.91 mmol, 1.15 equiv.) in DCM (39 mL) containing DMF
(5.26 mL, 68 mmol, 20 equiv.) and activated 4 Å MS (450 mg) at -78 °C. The suspension was stirred overnight while the temperature reached rt. TLC (tol/EtOAc, 70:30) indicated acceptor consumption and the presence of less polar products. Et<sub>3</sub>N (345 µL) was added and after 15 min, the suspension was filtered over a pad of Celite®. Solids were washed thoroughly with DCM and the organic phase was washed with sat. aq. NaHCO<sub>3</sub>, water and brine. The combined organic phases were dried over Na<sub>2</sub>SO<sub>4</sub> and concentrated to dryness. The crude was solubilized in DCM/MeOH (11:8, 48 mL), 25% methanolic MeONa (1.16 mL, 5.1 mmol, 1.5 equiv.) was added, and the solution was stirred overnight. Dowex H⁺ resin was added to the solution under gentle stirring until neutralisation. Filtration, concentration of the filtrate to dryness, and purification of the crude by flash chromatography (tol/EtOAc, 90:10) gave the known α anomer 24 (2.45 g, 88%).

**Route 2.** To a solution of rhamnoside 5 (1.0 g, 3.40 mmol, 1.0 equiv.) in MeCN (2.0 mL) were added trimethyl orthoacetate (0.7 mL, 6.45 mmol, 1.9 equiv.) and PTSA monohydrate (10 mg, 0.05 mmol, 0.015 equiv.) at rt. The orange mixture was stirred at rt for 1.5 h, and 80% aq. AcOH (2.0 mL) was added at 0 °C. After stirring for 30 min at 0 °C and at rt for 1 h, TLC (cHex/EtOAc, 80:20) indicated the total consumption of the intermediate orthoester. DCM and water were added and the two layers were separated. The aq. phase was extracted with DCM and the combined organic phases were washed successively with sat. aq. NaHCO<sub>3</sub> and brine, dried over Na<sub>2</sub>SO<sub>4</sub>, filtered and concentrated to dryness to give the crude acceptor 14. DMF (5.28 mL, 67.9 mmol, 20 equiv.) and activated 4 Å MS (0.5 g) were added to a mix of the latter and the PTFA donor 22 (3.14 g, 4.42 mmol, 1.3 equiv.) in anhyd. DCM (44 mL) and the suspension was stirred at rt under Ar for 30 min, then at -78 °C for 15 min. TfOH (0.3 mL, 3.40 mmol, 1.0 equiv.) was added very slowly at -78 °C. The reaction mixture was then stirred for 1 h while slowly warming up to rt. TLC (cHex/EtOAc, 70:30) showed the complete disappearance of rhamnoside 14 and the presence of less polar products. The reaction mixture was neutralized with Et<sub>3</sub>N. EtOAc was added along with water and the two layers were separated. The aq. phase was extracted with EtOAc and the combined organic layers were washed successively with sat. aq. NaHCO<sub>3</sub> and brine, dried over Na<sub>2</sub>SO<sub>4</sub>, filtered and evaporated. MeONa (25% in MeOH, 1.17 mL, 5.1 mmol, 1.5 equiv.) was added to the obtained crude in DCM/MeOH (11:8, 50 mL). After stirring at rt overnight, TLC (tol/EtOAc, 80:20) revealed that the glycosylation products had reacted and more polar products were present. DOWEX H⁺ resin was added and the mixture was stirred 30 minutes before filtering and washing thoroughly with MeOH. Et<sub>3</sub>N (few drops) was added and volatiles were evaporated. Purification by flash column chromatography (tol/EtOAc, 100:0 to 90:10) gave the desired α anomer 24 as a pale yellow oil (2.2 g, 79% over 3 steps) and the commercially available hemiacetal 6 (345 mg). The expected 24 had R<sub>f</sub> = 0.45 (cHex/EtOAc, 75:25). <sup>1</sup>H NMR (400 MHz, CDCl<sub>3</sub>) δ 7.47-7.17 (m, 25H, H<sub>Ar</sub>), 5.97 (m, 1H, CH=CH<sub>2</sub>), 5.36 (dq, J = 17.2 Hz, 1.5 Hz, 1H, CH=CH<sub>2</sub>), 5.27 (dq, J = 10.4 Hz, 1.6 Hz, 1H, CH=CH<sub>2</sub>), 5.06-4.97 (m, 4H,

H-1<sub>E</sub>, H-1<sub>A</sub>, 2H<sub>Bn</sub>), 4.91 (d, J = 11.0 Hz, 1H, H<sub>Bn</sub>), 4.90 (d, J = 11.6 Hz, 1H, H<sub>Bn</sub>), 4.84 (d, J = 10.7 Hz, 1H, H<sub>Bn</sub>), 4.78 (d, J = 11.6 Hz, 1H, H<sub>Bn</sub>), 4.72 (d, J = 10.7 Hz, 1H, H<sub>Bn</sub>), 4.59 (d, J = 12.2 Hz, 1H, H<sub>Bn</sub>), 4.57 (d, J = 11.0 Hz, 1H, H<sub>Bn</sub>), 4.38 (d, J = 12.2 Hz, 1H, H<sub>Bn</sub>), 4.23 (ddt, J = 13.1 Hz, 5.5 Hz, 1.5 Hz, 1H, CH<sub>2</sub>All), 4.14 (t, J = 9.3 Hz, 1H, H-3<sub>E</sub>), 4.12 (dd, J = 9.0 Hz, 3.2 Hz, 1H, H-3<sub>A</sub>), 4.08-4.00 (m, 3H, H-2<sub>A</sub>, H-5<sub>E</sub>, CH<sub>2</sub>All), 3.86 (dq, J = 9.6 Hz, 6.1 Hz, 1H, H-5<sub>A</sub>), 3.81 (t, J = 9.3 Hz, 1H, H-4<sub>E</sub>), 3.69 (dd, J = 9.6 Hz, 3.7 Hz, 1H, H-2<sub>E</sub>), 3.59 (t, J = 9.3 Hz, 1H, H-4<sub>A</sub>), 3.54 (dd, J = 11.0 Hz, 2.9 Hz, 1H, H-6<sub>B</sub>), 3.47 (brs, 1H, OH-2<sub>A</sub>), 3.43 (dd, J = 10.9 Hz, 2.0 Hz, 1H, H-6<sub>B</sub>), 1.45 (d, J = 6.3 Hz, 3H, H-6<sub>A</sub>). <sup>13</sup>C NMR (100 MHz, CDCl<sub>3</sub>) δ 138.7-137.6 (5C<sub>Ar</sub>), 133.9 (CH=CH), 128.7-127.6 (25CH<sub>Ar</sub>), 117.3 (CH=CH<sub>2</sub>), 98.3 (C-1<sub>A</sub>, J<sub>C,H</sub> = 170.1 Hz), 94.0 (C-1<sub>E</sub>, J<sub>C,H</sub> = 168.4 Hz), 82.5 (C-3<sub>E</sub>), 79.4 (C-4<sub>A</sub>), 79.1 (C-2<sub>E</sub>), 77.9 (C-4<sub>E</sub>), 76.7 (C-3<sub>A</sub>), 75.6 (2CH<sub>2</sub>Bn), 75.0 (CH<sub>2</sub>Bn), 74.3 (CH<sub>2</sub>Bn), 73.4 (CH<sub>2</sub>Bn), 70.8 (C-5<sub>E</sub>), 68.0 (C-6<sub>E</sub>), 67.9 (CH<sub>2</sub>All), 67.5 (C-2<sub>A</sub>), 67.3 (C-5<sub>A</sub>), 18.0 (C-6<sub>A</sub>). HRMS (ESI<sup>+</sup>): *m/z* 839.3749 (calcd for C<sub>50</sub>H<sub>56</sub>O<sub>10</sub>Na [M + Na]<sup>+</sup>: *m/z* 839.3766).

6-O-Acetyl-2,3,4-tri-O-benzyl-α/β-D-glucopyranose (27)<sup>60</sup>

Ac<sub>2</sub>O/TFA (4:1, 30 mL) was added to hemiacetal 6 (2.5 g, 5.0 mmol, 1.0 equiv.) at 0 °C and the suspension under Ar was stirred at rt for 3 h. TLC (cHex/EtOAc, 60:40) showed the conversion of the starting 6 into less polar products. Cold water (50 mL) was added at 0 °C and the mixture was stirred for 15 min at rt, then neutralized with sat. aq. NaHCO<sub>3</sub>. EtOAc was added and the two layers were separated. The aq. phase was extracted repeatedly with EtOAc and the combined organic layers were washed with sat. aq. NaHCO<sub>3</sub>, brine and dried over Na<sub>2</sub>SO<sub>4</sub>. Volatiles were evaporated and the crude was solubilized in DMF (20 mL). Hydrazine (60% in water, 0.22 mL, 7.0 mmol, 1.5 equiv.) and AcOH (0.40 mL, 7.0 mmol, 1.5 equiv.) were added at rt and the mixture was stirred at this temperature for 20 h. TLC (cHex/EtOAc, 60:40) showed the conversion of the 1,6-di-O-acetyl intermediate into more polar products. EtOAc and water were added and the two layers were separated. The aq. layer was extracted repeatedly with EtOAc and the combined organic phases were washed with sat. aq. NaHCO<sub>3</sub>, brine and dried over Na<sub>2</sub>SO<sub>4</sub>. Flash column chromatography on silica gel (cHex/EtOAc, 100:0 to 50:50) gave the known hemiacetal 27 as a white solid (α/β 65:35, 1.99 g, 87%). An analytical sample was obtained by means of a second purification. <sup>1</sup>H NMR (400 MHz, DMSO-d<sub>6</sub>) δ 7.43-7.23 (m, 15H, H<sub>Ar</sub>), 5.25 (d, J = 3.4 Hz, 0.65H, H-1<sub>α</sub>), 4.96-4.52 (m, 6.35H, H<sub>Bn</sub>, H-1<sub>β</sub>), 4.30-4.10 (m, 2H, H-6), 3.96 (ddd, J = 10.1, 4.8, 2.5 Hz, 0.65H, H-5<sub>α</sub>), 3.90 (t, J = 9.2 Hz, 0.65H, H-3<sub>α</sub>), 3.72-3.59 (m, 0.7H, H-3<sub>β</sub>, H-5<sub>β</sub>), 3.49-3.38 (m, 1.65H, H-2<sub>α</sub>, H-4<sub>α</sub>, H-4<sub>β</sub>), 3.26 (dd, J = 9.2, 7.8 Hz, 0.35H, H-2<sub>β</sub>), 2.03 (s, 1.05H, Ac<sub>β</sub>), 2.00 (s, 1.95H, Ac<sub>α</sub>). <sup>13</sup>C NMR (100 MHz, DMSO-d<sub>6</sub>) δ 139.30 (C<sub>Arα</sub>), 139.27 (C<sub>Arβ</sub>), 139.2 (C<sub>Arβ</sub>), 139.1 (C<sub>Arα</sub>), 138.8 (C<sub>Arα</sub>), 138.7 (C<sub>Arβ</sub>), 128.7-127.8 (15CH<sub>Ar</sub>), 97.2 (C-1<sub>β</sub>), 90.1 (C-1<sub>α</sub>), 84.3 (C-3<sub>β</sub>), 83.5 (C-2<sub>β</sub>), 81.4 (C-3<sub>α</sub>), 80.5 (C-2<sub>α</sub>), 78.1 (C-4), 74.9-74.0 (2CH<sub>2</sub>Bn), 72.4 (C-5<sub>β</sub>), 71.8 (CH<sub>2</sub>Bn), 68.2 (C-5<sub>α</sub>), 63.64 (C-6<sub>β</sub>), 63.61 (C-6<sub>α</sub>), 21.1 (OCH<sub>3</sub>). HRMS (ESI<sup>+</sup>): *m/z* 510.2479 (calcd for C<sub>29</sub>H<sub>36</sub>NO<sub>7</sub> [M + NH<sub>4</sub>]<sup>+</sup>: *m/z* 510.2486).

<figure><img src="image_23.png" /></figure>
## 6-O-Acetyl-2,3,4-tri-O-benzyl-α/β-D-glucopyranosyl (N-phenyl) trifluoroacetimidate (29)

K₂CO₃ (0.53 g, 3.86 mmol, 2.0 equiv.) and (N-phenyl)trifluoroacetimidoyl chloride (0.46 mL, 2.89 mmol, 1.5 equiv.) were added to hemiacetal 27 (950 mg, 1.93 mmol, 1.0 equiv.) in acetone (20 mL) stirred at rt. The suspension was stirred at this temperature for 2 h. TLC (cHex/EtOAc, 80:20) revealed the conversion of the starting 27 into less polar products. The suspension was filtered over a pad of Celite®, generously washed with DCM, and volatiles were evaporated. Purification of the residue by flash column chromatography on silica gel (cHex/EtOAc, 100:0 to 95:5) gave (N-phenyl)trifluoroacetimidate 29 as a 1:1 mix of α/β anomers (1.1 g, 90%). An analytical sample was obtained by means of a second purification. Donor 29 had R<sub>f</sub> = 0.6 (cHex/EtOAc, 90:10). ¹H NMR (400 MHz, CDCl<sub>3</sub>) δ 7.44-7.21 (m, 17H, H<sub>Ar</sub>), 7.12 (m, 1H, H<sub>Ar</sub>), 6.78-6.73 (m, J = 7.7 Hz, 2H, H<sub>Ar</sub>), 6.45 (brs, 1H, H-1), 5.04 (d, J = 10.8 Hz, 1H, H<sub>Bn</sub>), 4.92 (d, J = 10.8 Hz, 1H, H<sub>Bn</sub>), 4.89 (d, J = 10.8 Hz, 1H, H<sub>Bn</sub>), 4.81-4.75 (m, 2H, H<sub>Bn</sub>), 4.62 (d, J = 10.8 Hz, 1H, H<sub>Bn</sub>), 4.38-4.25 (m, 2H, H-6), 4.08 (t<sub>app</sub>, J = 9.3 Hz, 1H, H-3), 4.02 (m, 1H, H-5), 3.73 (dd, J = 9.3, 3.4 Hz, 1H, H-2), 3.61 (t<sub>app</sub>, J = 9.5 Hz, 1H, H-4), 2.06 (s, 3H, CH<sub>3</sub>Ac). ¹³C NMR (100 MHz, CDCl<sub>3</sub>) δ 170.5 (CO<sub>Ac</sub>), 143.5-137.6 (4CH<sub>Ar</sub>), 128.7-127.7 (19CH<sub>Ar</sub>), 124.2 (CF<sub>3</sub>), 119.4 (CH<sub>Ar</sub>), 81.5 (C-3), 79.3 (C-2), 77.2 (C-4), 75.8 (CH<sub>2</sub>Bn), 75.3 (CH<sub>2</sub>Bn), 73.4 (CH<sub>2</sub>Bn), 71.5 (C-5), 62.6 (C-6), 20.8 (CH<sub>3</sub>Ac). (C-1 and C=N could not be detected due to relaxation issues). HRMS (ESI<sup>+</sup>): *m/z* 681.2778 (calcd for C<sub>37</sub>H<sub>40</sub>F<sub>3</sub>N<sub>2</sub>O<sub>7</sub> [M + NH<sub>4</sub>]<sup>+</sup>: *m/z* 706.3558).

## 2,3,4-Tri-O-benzyl-6-O-tert-butyldiphenylsilyl-α/β-D-glucopyranose (30)<sup>61</sup>

A mixture of Ac₂O/TFA (4:1, 30 mL) was added at 0 °C to hemiacetal 6 (2.5 g, 5.0 mmol, 1.0 equiv.) under Ar and the suspension was stirred at rt for 3 h, at which time TLC (cHex/EtOAc, 60:40) indicated the conversion of the starting 6 into less polar products. Cold water (50 mL) was added at 0 °C and the mixture was stirred for 15 min at this temperature, then neutralized with 4 M aq. NaOH. EtOAc was added and the two layers were separated. The aq. layer was extracted with EtOAc and the combined organic phases were washed with brine and dried over Na₂SO₄. Volatiles were evaporated and MeONa (25% in MeOH, 5 mL) was added to the crude intermediate stirred in MeOH (20 mL) at rt. After stirring overnight at this temperature, TLC (cHex/EtOAc, 60:40) showed the complete disappearance of the intermediate and the presence of more polar products. Dowex H⁺ resin was added portion-wise while the suspension was gently stirred until neutralisation. The suspension was filtered and the volatiles were evaporated. DMAP (0.11 g, 0.93 mmol, 0.2 equiv.), imidazole (0.76 g, 11.1 mmol, 2.4 equiv.) and tert-butyldiphenylsilyl chloride (TBDPSCl, 1.44 mL, 5.55 mmol, 1.2 equiv.) were added to a solution of the crude in DMF (40 mL) at 0 °C. The mixture was stirred overnight at rt, at which time more imidazole (2.0 equiv.) and TBDPSCl (1.1 equiv.) were added. After 3 h, water and Et₂O were added and the two layers were separated. The aq. phase

was extracted with Et₂O and the combined organic phases were washed with sat. aq. NaHCO₃, brine and dried over Na₂SO₄. Volatiles were evaporated under reduced pressure; two successive purifications by flash column chromatography on silica gel (cHex/EtOAc, 100:0 to 95:5) gave hemiacetal 30 as a colorless oil (mostly α/β mixture, 1.7 g) contaminated with tert-butyldiphenylsilanol (10 mol%). Only the major isomer is described. ¹H NMR (400 MHz, DMSO-d₆) δ 7.85-7.09 (m, 25H, H<sub>Ar</sub>), 5.34 (d, J = 3.4 Hz, 1H, H-1), 5.00-4.56 (m, 6H, H<sub>Bn</sub>), 4.04-3.74 (m, 4H, H-3, H-5, H-6), 3.70-3.60 (m, 1H, H-4), 3.47 (dt, J = 9.6, 2.8 Hz, 1H, H-2), 1.04-0.96 (m, 9H, Si(CH₃)₃). ¹³C NMR (100 MHz, DMSO-d₆) δ 139.5-138.8 (5C<sub>Ar</sub>), 135.8-126.9 (25CH<sub>Ar</sub>), 90.3 (C-1), 81.5 (C-3), 80.9 (C-2), 78.04 (C-4), 74.9-71.1 (3CH<sub>2</sub>Bn), 70.8 (C-5), 63.4 (C-6), 27.2 (SiC(CH₃)₃), 19.4 (SiC(CH₃)₃). HRMS (ESI<sup>+</sup>): *m/z* 706.3548 (calcd for C₄₃H₅₂NO₆Si [M + NH₄]<sup>+</sup>: *m/z* 706.3558).

## 2,3,4-Tri-O-benzyl-6-O-tert-butyldiphenylsilyl-α/β-D-glucopyranosyl (N-phenyl)trifluoroacetimidate (32)

K₂CO₃ (0.32 g, 2.32 mmol, 2.0 equiv.) and (N-phenyl)trifluoroacetimidoyl chloride (PTFACl, 0.28 mL, 1.74 mmol, 1.5 equiv.) were added to hemiacetal 30 (800 mg, 1.16 mmol, 1.0 equiv.) in acetone (11.6 mL) at rt. After stirring at rt for 20 h, TLC (cHex/EtOAc, 90:10) indicated the conversion of hemiacetal 30 into less polar products. The suspension was filtered over a pad of Celite®, and the solids were generously washed with DCM. Volatiles were evaporated and the residue was purified by flash column chromatography (cHex/EtOAc, 100:0 to 90:10) to give the (N-phenyl)trifluoroacetimidate 32 as a 55:45 mix of α/β anomers (952 mg, 40% over four steps). Donor 32 had R<sub>f</sub> = 0.65 (cHex/EtOAc, 90:10). ¹H NMR (400 MHz, CDCl₃) δ 7.83-7.63 (m, 4H, H<sub>Ar</sub>), 7.50-7.21 (m, 23H, H<sub>Ar</sub>), 7.14 (m, 1H, H<sub>Ar</sub>), 6.82-6.72 (m, 2H, H<sub>Ar</sub>), 6.55 (s, 0.55H, H-1<sub>a</sub>), 6.29 (s, 0.45H, H-1<sub>b</sub>), 5.12-4.89 (m, 2H, H<sub>Bn</sub>), 4.85-4.63 (m, 4H, H<sub>Bn</sub>), 4.33 (t, J = 9.6 Hz, 0.45H, H-4<sub>a</sub>), 4.18-3.81 (m, 5H, H-2<sub>a</sub>, H-3, H-4<sub>b</sub>, H-5, H-6), 3.76 (m, 0.55H, H-2<sub>b</sub>), 1.11 (s, 9H, SiC(CH₃)₃). ¹³C NMR (100 MHz, CDCl₃) δ 143.8-137.9 (4C<sub>Ar</sub>), 135.9-135.6 (4CH<sub>Ar</sub>), 133.9-133.1 (2C<sub>Ar</sub>), 129.7-124.1 (25CH<sub>Ar</sub>), 124.3 (CF₃<sub>a</sub>), 124.1 (CF₃<sub>b</sub>), 119.5 (CH<sub>Ar</sub>), 81.6 (C-3<sub>a</sub>), 79.7 (C-2<sub>b</sub>), 79.1 (C-3<sub>b</sub>), 77.2, 76.9, 75.9 (CH<sub>2</sub>Bn<sub>a</sub>), 75.8, 75.4 (CH<sub>2</sub>Bn), 73.9 (C-4<sub>a</sub>), 73.4 (CH<sub>2</sub>Bn<sub>b</sub>), 72.8 (CH<sub>2</sub>Bn<sub>a</sub>), 72.8 (CH<sub>2</sub>Bn<sub>b</sub>), 62.7 (C-6<sub>a</sub>), 62.4 (C-6<sub>b</sub>), 26.9 (SiC(CH₃)₃<sub>a</sub>), 26.8 (SiC(CH₃)₃<sub>b</sub>), 19.4 (SiC(CH₃)₃<sub>a</sub>), 19.4 (SiC(CH₃)₃<sub>b</sub>) (C-1 and C=N could not be detected due to relaxation). HRMS (ESI<sup>+</sup>): *m/z* 877.3853 (calcd for C₅₁H₅₆F₃N₂O₆Si [M + NH₄]<sup>+</sup>: *m/z* 877.3854).

## (2,3,4,6-Tetra-O-benzyl-α-D-glucopyranosyl)-(1 → 3)-4-O-benzyl-2-O-levulinoyl-α-L-rhamnopyranosyl (N-phenyl) trifluoroacetimidate (35)

Levulinic acid (9.7 g, 83 mmol, 2.0 equiv.), EDC (15.5 g, 75 mmol, 1.8 equiv.) and DMAP (3.4 g, 17 mmol, 0.4 equiv.) were added to alcohol 24 (34 g, 42 mmol, 1.0 equiv.) in anhyd. DCM (210 mL). The mixture was stirred at rt for 60 h, at which time TLC (tol/EtOAc, 80:20) showed the full consumption of the starting 24 and the presence of a more polar product. The reaction mixture was diluted with water and DCM. The two

<figure><img src="image_24.png" /></figure>
<figure><img src="image_25.png" /></figure>

layers were separated and the aq. phase was extracted with DCM repeatedly. The combined organic layers were washed successively with sat. aq. NaHCO₃, water and finally brine. The organic layer was dried over Na₂SO₄, filtered, and volatiles were evaporated. PdCl₂ (621 mg, 2.1 mmol, 0.05 equiv., 60% purity) was added to the crude 3 in DCM/H₂O (3:1, 420 mL). The biphasic mixture was stirred at 50 °C for 3 h. TLC (tol/ EtOAc, 80:20) showed conversion of the starting 3 into a less polar product. Iodine (10.7 g, 84 mmol, 2.0 equiv.) in THF (50 mL) was added slowly to the solution at rt. After stirring at this temperature for 2.5 h, TLC (tol/ EtOAc, 8:2) showed the conversion of the intermediate into a more polar product. Sat. aq. Na₂S₂O₃ was added and the biphasic mixture was filtered over a pad of Celite®. The organic phase was washed with sat. aq. NaHCO₃, water and brine. The organic phase was dried over Na₂SO₄, filtered, and concentrated to dryness. PTFACl (10 mL, 63 mmol, 1.5 equiv.) and K₂CO₃ (11.6 g, 84 mmol, 2.0 equiv.) were added slowly to the crude hemiacetal in acetone (420 mL) under Ar, at rt. The suspension was stirred at this temperature for 16 h. TLC (cHex/EtOAc, 70:30) showed the consumption of the intermediate 33 and the presence of less polar products. After filtration over a pad of Celite®, thorough washing of the solids with DCM and concentration of the filtrate to dryness, the residue was purified by column chromatography (cHex/EtOAc, 100:0 to 80:20) to give donor 35 (39.5 g, 90% over 3 steps, 9:1 mix of anomers) as an orange oil. The PTFA donor 35 had R<sub>f</sub> = 0.8 (tol/EtOAc, 70:30). <sup>1</sup>H NMR (400 MHz, CDCl<sub>3</sub>) δ 7.48-7.25 (m, 23H, H<sub>Ar</sub>), 7.21-7.11 (m, 5H, H<sub>Ar</sub>), 6.99-6.84 (m, 2H, H<sub>Ar</sub>), 6.20 (brs, 0.89H, H-1<sub>Aα</sub>), 5.95 (brs, 0.11H, H-1<sub>Aβ</sub>), 5.62 (t<sub>app</sub>, J = 3.2 Hz, 1H, H-2<sub>A</sub>), 5.29 (d, J = 3.5 Hz, 1H, H-1<sub>E</sub>), 5.06 (d, J = 11.1 Hz, 1H, H<sub>Bn</sub>), 5.03 (d, J = 10.4 Hz, 1H, H<sub>Bn</sub>), 4.95 (d, J = 11.1 Hz, 1H, H<sub>Bn</sub>), 4.92 (d, J = 10.9 Hz, 1H, H<sub>Bn</sub>), 4.83 (d, J = 12.1 Hz, 1H, H<sub>Bn</sub>), 4.77 (d, J = 12.1 Hz, 1H, H<sub>Bn</sub>), 4.69 (d, J = 9.9 Hz, 1H, H<sub>Bn</sub>), 4.65 (d, J = 12.0 Hz, 1H, H<sub>Bn</sub>), 4.55 (d, J = 10.9 Hz, 1H, H<sub>Bn</sub>), 4.44 (d, J = 12.1 Hz, 1H, H<sub>Bn</sub>), 4.33 (dd, J = 9.6, 3.2 Hz, 1H, H-3<sub>A</sub>), 4.16 (t, J = 9.3 Hz, 1H, H-3<sub>E</sub>), 4.10 (m, 1H, H-5<sub>E</sub>), 3.97 (dq, J = 12.2, 6.3 Hz, 1H, H-5<sub>A</sub>), 3.84 (t, J = 9.5 Hz, 1H, H-4<sub>E</sub>), 3.69 (m, 3H, H-2<sub>E</sub>, H-4<sub>A</sub>, H-6<sub>A</sub>), 3.59 (dd, J = 10.9, 2.1 Hz, 1H, H-6<sub>B</sub>), 2.69-2.42 (m, 4H, CH<sub>2Lev</sub>), 2.11 (s, 3H, CH<sub>3Lev</sub>), 1.48 (d, J = 6.2 Hz, 3H, H-6<sub>A</sub>). <sup>13</sup>C NMR (100 MHz, CDCl<sub>3</sub>) δ 205.9 (CO<sub>Lev</sub>), 171.8 (CO<sub>2Lev</sub>), 143.3-137.4 (6C<sub>Ar</sub>), 128.8-127.49 (29CH<sub>Ar</sub>), 124.5 (CF<sub>3</sub>), 119.5 (CH<sub>Ar</sub>), 94.0 (C-1<sub>A</sub>), 93.4 (C-1<sub>E</sub>, J<sub>C,H</sub> = 170.2 Hz), 82.1 (C-3<sub>E</sub>), 79.4 (C-2<sub>E</sub> or C-4<sub>A</sub>), 79.1 (C-2<sub>E</sub> or C-4<sub>A</sub>), 77.8 (C-4<sub>E</sub>), 76.4 (CH<sub>2Bn</sub>), 75.6 (CH<sub>2Bn</sub>), 75.1 (CH<sub>2Bn</sub>), 73.4 (CH<sub>2Bn</sub>), 73.0 (CH<sub>2Bn</sub>), 72.1 (C-3<sub>A</sub>), 70.7 (C-5<sub>A</sub>), 70.5 (C-5<sub>E</sub>), 68.1 (C-6<sub>E</sub>), 66.8 (C-2<sub>A</sub>), 37.8 (CH<sub>2Lev</sub>), 29.7 (CH<sub>3Lev</sub>), 28.0 (CH<sub>2Lev</sub>), 18.0 (C-6<sub>A</sub>). HRMS (ESI<sup>+</sup>): *m/z* 1063.4580 (calcd for C<sub>60</sub>H<sub>66</sub>F<sub>3</sub>N<sub>2</sub>O<sub>12</sub> [M + NH<sub>4</sub>]<sup>+</sup>: *m/z* 1063.4568*.

Allyl (2,3,4,6-tetra-*O*-benzyl-*α*-d-glucopyranosyl)-(1 → 3)-(4-*O*-benzyl-2-*O*-levulinoyl-*α*-L-rhamnopyranosyl)-(1 → 2)-(3,4-di-*O*-benzyl-*α*-L-rhamnopyranosyl)-(1 → 3)-(2-*O*-acetyl-4-*O*-benzyl-*α*-L-rhamnopyranosyl)-(1 → 3)-4,6-*O*-benzylidene-2-deoxy-2-trichloroacetamido-*β*-d-glucopyranoside (1)¹⁷

Hydrazine hydrate (60% in water, 5.0 mL, 103 mmol, 5.0 equiv.) was added dropwise to a solution of the fully pro-

tected BₐcCD¹⁷ 2 (23.8 g, 20.6 mmol, 1.0 equiv.) in pyridine/ AcOH (3:2, 410 mL) at 0 °C. The reaction mixture was stirred at rt for 4 h. TLC (tol/EtOAc, 8:2) showed the complete disappearance of the starting material and the presence of a more polar product. The reaction mixture was diluted with water and EtOAc. The two layers were separated, the aq. phase was extracted with EtOAc and the combined organic phases were washed with sat. aq. NaHCO₃, water and brine, dried over Na₂SO₄, filtered and evaporated to dryness. Filtration on a pad of silica (tol/EtOAc, 8:2) afforded the desired compound as a white foam. Activated 4 Å MS (5.0 g) was added to the crude acceptor **31** (20.6 mmol, 1.0 equiv.) and donor **35** (24.8 g, 23.7 mmol, 1.15 equiv.) in anhyd. toluene (410 mL) and the suspension was stirred at rt, under Ar, for 30 min. TMSOTf (750 µL, 4.12 mmol, 0.2 equiv.) was added very slowly at rt. After stirring at rt for 1 h, TLC (tol/ acetone, 80:20) showed the complete disappearance of acceptor **31** and the presence of less polar products. Et₃N was added and after stirring for 30 min at rt, solids were filtered over a pad of Celite® and washed generously with DCM. Successive purifications by flash column chromatography on silica gel (tol/acetone, 100:0 to 90:10 then tol/ EtOAc, 100:0 to 80:20) afforded the desired pentasaccharide 1 as a beige foam (31.5 g, 80% over 2 steps).

Allyl (3,4-di-*O*-benzyl-*α*-L-rhamnopyranosyl)-(1 → 3)-(2-*O*-acetyl-4-*O*-benzyl-*α*-L-rhamnopyranosyl)-(1 → 3)-4,6-*O*-benzylidene-2-deoxy-2-trichloroacetamido-*β*-d-glucopyranoside (36)¹⁷

**Route 1.** Hydrazine hydrate (0.36 mL, 7.4 mmol) was added to a solution of the fully protected BₐcCD¹⁷ 2 (4.29 g, 3.7 mmol) in pyridine/AcOH (3:2 v/v, 74 mL) and the resulting solution was stirred at rt under Ar for 1 h. TLC (CH₂Cl₂/MeCN, 90:10) showed the complete conversion of the starting material into a closely migrating more polar compound. The mixture was partitioned between water (200 mL) and CH₂Cl₂ (200 mL). The aq. layer was extracted with CH₂Cl₂ (100 mL twice). The combined organic layers were washed with sat. aq. NaHCO₃ (300 mL) and brine (300 mL), dried on Na₂SO₄, filtered and concentrated under reduced pressure. Flash chromatography on silica gel of the crude (CH₂Cl₂/MeCN, 95:5 to 85:15) afforded the alcohol **36** (3.6 g, 92%) as a white foam. The latter had R<sub>f</sub> = 0.4 (CH₂Cl₂/MeCN, 90:10).

**Route 2.** Acetic acid (27.8 mL) and ethylenediamine (775 µL, 6.9 mmol, 5.0 equiv.) were successively added at 0 °C to trisaccharide 2 (1.6 g, 1.38 mmol, 1.0 equiv.) in pyridine (42 mL). The reaction mixture was heated to 70 °C for 24 h. At this time, a <sup>1</sup>H NMR control showed reaction completion. The solution was diluted with DCM (600 mL) and washed with water (300 mL). The aqueous phase was extracted with DCM (300 mL) and the combined organic phases were washed with brine (300 mL), dried over sodium sulfate, filtered and concentrated under vacuum. The crude product was purified by flash column chromatography (tol/ EtOAc, 80:20) to give, in order of elution, the remaining 2 (208 mg, 13%) and the acceptor **36** (1.08 g, 74%) as a white foam.
Allyl (2,3,4,6-tetra-O-benzyl-α-D-glucopyranosyl)-(1 → 3)-(4-O-benzyl-α-L-rhamnopyranosyl)-(1 → 2)-(3,4-di-O-benzyl-α-L-rhamnopyranosyl)-(1 → 3)-(2-O-acetyl-4-O-benzyl-α-L-rhamnopyranosyl)-(1 → 3)-4,6-O-benzylidene-2-deoxy-2-trichloroacetamido-β-D-glucopyranoside (37)

Hydrazine hydrate (50 µL, 1.0 mmol, 2 equiv.) was added to the pentasaccharide 1 (1.0 g, 0.52 mmol) in pyridine/AcOH (3:2, 10 mL) and the solution was stirred at rt under Ar for 30 min. TLC (cHex/EtOAc, 70:30) showed the complete conversion of the starting material into a less polar compound. Water (100 mL) and EtOAc (100 mL) were added and the phases were separated. The aqueous layer was extracted with EtOAc (50 mL twice) and the combined organic layers were washed with sat. aq. NaHCO₃ and brine, then dried on Na₂SO₄, filtered, and concentrated under reduced pressure. Flash chromatography of the crude residue (cHex/EtOAc, 90:10 to 50:50) gave alcohol 37 (865 mg, 92%) as a white foam.

**Route 2.** Acetic acid (10.5 mL) and ethylenediamine (583 µL, 5.2 mmol, 10.0 equiv.) were successively added at 0 °C to pentasaccharide 1 (1.0 g, 520 µmol, 1.0 equiv.) in pyridine (15.7 mL). The reaction mixture was heated to 70 °C and for 24 h. At this time, a ¹H NMR control showed reaction completion. The solution was diluted with DCM (400 mL) and washed with water (200 mL). The aqueous phase was extracted with DCM (200 mL) and the combined organic phases were washed with brine (300 mL), dried over sodium sulfate, filtered and concentrated under vacuum. The crude product was purified by flash column chromatography (tol/EtOAc, 80:20) to give pentasaccharide 37 (841 mg, 89%) as a white foam. The latter had $R_f = 0.3$ (tol/EtOAc, 70:30). ¹H NMR (400 MHz, CDCl₃) $δ$ 7.52–7.12 (m, 45H, $H_{Ar}$), 7.02 (d, $J = 7.4$ Hz, 1H, $NHCO$), 5.93–5.82 (m, 1H, $CH=CH_2$), 5.56 (s, 1H, $H_{BzL}$), 5.34–5.18 (m, 2H, $CH=CH_2$), 5.16 (dd, $J = 3.3, 1.9$ Hz, 1H, $H-2C$), 5.13 (brs, 1H, $H-1A$), 5.12 (d, $J = 8.5$ Hz, 1H, $H-1D$), 4.99–4.81 (m, 8H, $H-1B$, $H-1E$, $H-1C$, 5H$_{Bn}$), 4.78–4.28 (m, 14H, $H-3D$, $H-6bD$, $CH_{2All}$, 11H$_{Bn}$), 4.14–4.07 (m, 1H, $CH_{2All}$), 4.07–3.91 (m, 7H, $H-3E$, $H-2A$, $H-3A$, $H-2B$, $H-3C$, $H-5C$, $H-5E$), 3.86–3.55 (m, 8H, $H-5A$, $H-4A$, $H-6aD$, $H-4E$, $H-5B$, $H-4B$, $H-5D$, $H-2E$), 3.53–3.36 (m, 7H, $H-3B$, $H-4D$, $H-2D$, $H-6aE$, $H-6bE$), 3.28 ($t_{app}$, $J = 9.5$ Hz, 1H, $H-4C$), 2.06 (s, 3H, $OCOCH_3$), 1.68 (bs, 1H, $OH$), 1.26 (d, $J = 6.2$ Hz, 3H, $H-6A$), 1.25 (d, $J = 6.2$ Hz, 3H, $H-6B$), 0.73 (d, $J = 6.2$ Hz, 3H, $H-6C$). ¹³C NMR (100 MHz, CDCl₃) $δ$ 169.9 ($OCOCH_3$), 162.3 ($CONH$), 133.4 ($CH=CH_2$), 138.8–137.1 (9$C_{Ar}$), 129.2–126.6 (45$CH_{Ar}$), 118.5 ($CH=CH_2$), 102.1 ($C_{BzL}$), 101.5 (C-1$_B$, $J_{C,H} = 170.7$ Hz), 100.8 (C-1$_A$, $J_{C,H} = 173.8$ Hz), 98.3 (C-1$_D$, $J_{C,H} = 168.9$ Hz), 97.6 (C-1$_C$, $J_{C,H} = 173.5$ Hz), 94.1 (C-1$_E$, $J_{C,H} = 170.2$ Hz), 92.2 (CCl₃), 82.6 (C-3$_E$), 80.4 (C-4$_D$), 80.3 (C-4$_B$), 80.1 (C-4$_C$), 79.8 (C-4$_A$), 79.4 (C-3$_B$), 79.0 (C-2$_E$), 78.5 (C-3$_C$), 77.9 (C-4$_E$), 76.6 (C-2$_B$), 75.8 (2C, $CH_{2Bn}$), 75.4 ($CH_{2Bn}$), 75.3 ($CH_{2Bn}$), 75.1 ($CH_{2Bn}$), 75.0 (C-3$_A$), 74.6 ($CH_{2Bn}$), 74.0 (C-3$_D$), 73.6 ($CH_{2Bn}$), 72.5 ($CH_{2Bn}$), 72.3 (C-2$_C$), 71.1 ($CH_{2All}$), 70.9 (C-5$_E$), 69.1 (C-5$_B$), 68.9 (C-6$_D$), 68.1 (C-5$_C$), 68.0 (C-6$_E$), 67.9 (C-5$_A$), 67.4 (C-2$_A$), 66.4 (C-5$_D$), 60.4 (C-2$_D$), 21.2 ($OCOCH_3$), 18.0 (2C, C-6$_A$, C-6$_B$), 17.4 (C-6$_C$). HRMS (ESI⁺): *m/z* 1831.6810 (calcd for C₁₀₀H₁₁₀Cl₃NO₂₄NH₄ [M + NH₄]⁺: *m/z* 1831.6822).

(2,3,4,6-Tetra-O-benzyl-α-D-glucopyranosyl)-(1 → 3)-(4-O-benzyl-2-O-levulinoyl-α-L-rhamnopyranosyl)-(1 → 2)-(3,4-di-O-benzyl-α-L-rhamnopyranosyl)-(1 → 3)-(2-O-acetyl-4-O-benzyl-α-L-rhamnopyranosyl)-(1 → 3)-4,6-O-benzylidene-2-deoxy-2-trichloroacetamido-α/β-D-glucopyranose (38)

1,5-Cyclooctadiene-bis(methyldiphenylphosphine)iridium hexafluorophosphate (62 mg, 73 µmol, 0.02 equiv.) was dissolved in anhyd. THF (1.5 mL) and the resulting red suspension was degassed and filled up with argon. Hydrogen was bubbled through the solution for 10 min, causing the color to change to yellow. The solution was degassed again, saturated with argon, and transferred to a solution of pentasaccharide 1 (7.02 g, 3.67 mmol, 1.0 equiv.) in anhyd. THF (18.3 mL) under argon at rt. The mixture was stirred at rt for 1 h. TLC (tol/EtOAc, 80:20, double migration) showed the complete disappearance of the starting material and the presence of a closely migrating less polar product. Iodine (2.33 g, 18.34 mmol, 5 equiv.) in THF/water (9:1, 24 mL) and solid NaHCO₃ (3.08 g, 36.67 mmol, 10 equiv.) were added and the mixture was stirred vigorously at rt for 30 min. TLC (tol/EtOAc, 80:20, cHex/EtOAc, 60:40) showed the complete disappearance of the intermediate and the presence of a more polar product. Excess iodine was destroyed by adding 10% aq. Na₂S₂O₃ until the color was stable. THF was evaporated under reduced pressure and EtOAc (300 mL) was added. The organic layer was washed with sat. NaHCO₃, water, and brine, dried on Na₂SO₄, filtered, and concentrated under reduced pressure. Flash column chromatography of the residue (tol/EtOAc, 90:10 to 70:30) gave hemiacetal 38 (α/β: 90:10, 6.25 g, 92%) as a white foam. The latter had $R_f = 0.5$ (tol/EtOAc, 80:20). ¹H NMR (400 MHz, CDCl₃) $δ$ 7.52–7.06 (m, 45H, $H_{Ar}$), 6.94 (d, $J = 9.4$ Hz, 1H, $H_{NHCO}$), 5.56 (s, 1H, $H_{BzL}$), 5.55 (brs, 1H, $H-2A$), 5.25 (d, $J = 3.2$ Hz, 1H, $H-1E$), 5.20 (dd, $J = 7.1, 3.4$ Hz, 1H, $H-1Dα$), 5.11 (dd, $J = 3.3, 1.9$ Hz, 1H, $H-2C$), 5.04–4.57 (m, 17H, $H-1A$, $H-1B$, $H-1C$, $H-3D$, 13H$_{Bn}$), 4.50 (d, $J = 11.1$ Hz, 1H, $H_{Bn}$), 4.44 (d, $J = 11.5$ Hz, 1H, $H_{Bn}$), 4.38–4.22 (m, 4H, $H_{Bn}$, $H-6aD$, $H-2D$, $H-3A$), 4.17–4.03 (m, 5H, $H-5D$, $H-3E$, $H-2B$, $H-3C$, $H-5E$), 4.00–3.46 (m, 14H, $H-5C$, $H-3B$, $H-4E$, $H-5A$, $H-6bD$, $H-2E$, $H-4A$, $H-4B$, $H-4D$, $OH$, $H-5B$, $H-5D$, $H-6aE$, $H-6bE$), 3.29 ($t_{app}$, $J = 9.5$ Hz, 1H, $H-4C$), 2.62–2.35 (m, 4H, $CH_{2Lev}$), 2.08 (s, 3H, $CH_{3Lev}$), 2.07 (s, 3H, $OCOCH_3$), 1.29 (d, $J = 6.2$ Hz, 6H, $H-6A$, $H-6B$), 0.78 (d, $J = 6.2$ Hz, 0.3H, $H-6C$), 0.74 (d, $J = 6.2$ Hz, 2.7H, $H-6C$). ¹³C NMR (100 MHz, CDCl₃) $δ$ 206.4 (CO$_{Lev}$), 171.8 (CO₂Lev), 169.8 ($OCOCH_3$), 162.1 (CONH), 138.9–137.1 (9$C_{Ar}$), 129.2–126.5 (45$CH_{Ar}$), 102.2 (C$_{BzL}$), 100.6 (C-1$_B$, $J_{C,H} = 170.7$ Hz), 99.2 (C-1$_A$, $J_{C,H} = 173.8$ Hz), 98.1 (C-1$_C$, $J_{C,H} = 173.5$ Hz), 92.9 (C-1$_E$, $J_{C,H} = 170.2$ Hz), 92.4 (CCl₃), 92.0 (C-1$_Dα$, $J_{C,H} = 170.2$ Hz), 82.2 (C-3$_E$), 80.4 (2C, C-4$_D$, C-4$_B$), 80.3 (C-4$_C$), 79.8 (C-4$_A$), 79.5 (C-2$_E$), 79.2 (C-3$_B$), 77.8 (C-4$_E$), 77.4 (C-3$_C$), 76.1 ($CH_{2Bn}$), 75.9 (C-2$_B$), 75.6 ($CH_{2Bn}$), 75.4 (2C, C-3$_D$, $CH_{2Bn}$), 75.3 ($CH_{2Bn}$), 75.1 ($CH_{2Bn}$), 75.0 ($CH_{2Bn}$), 73.5 ($CH_{2Bn}$), 72.8 ($CH_{2Bn}$), 72.4 (C-2$_C$), 72.3 (C-3$_A$), 72.1 ($CH_{2Bn}$), 70.3 (C-5$_E$), 69.0 (2C, C-5$_B$, C-6$_D$), 68.7 (C-5$_A$), 68.4 (C-6$_E$), 68.2 (C-2$_A$), 68.1 (C-5$_C$), 63.2 (C-5$_D$), 55.6 (C-2$_D$), 38.0 ($CH_{2Lev}$), 29.8 ($CH_{3Lev}$), 28.2 ($CH_{2Lev}$), 21.1 ($OCOCH_3$), 18.0 (C-6$_A$), 17.9 (C-6$_B$), 17.4 (C-6$_C$). HRMS (ESI⁺): *m/z* 1891.6873 (calcd for C₁₀₂H₁₁₆Cl₃N₂O₂₆ [M + NH₄]⁺: *m/z* 1891.6886).

<figure><img src="image_26.png" /></figure>
Research Article

### (α-L-Rhamnopyranosyl)-(1 → 3)-4,6-O-benzylidene-2-deoxy-2-trichloroacetamido-α/β-D-glucopyranosyl (N-phenyl) trifluoroacetimidate (39)

To a solution of hemiacetal 37 (7.04 g, 3.76 mmol) in acetone (75 mL) at rt were added sequentially $K_2CO_3$ (1.56 g, 11.27 mmol, 3 equiv.) and PTFACl (01.19 mL, 7.51 mmol, 2 equiv.). The suspension was stirred vigorously overnight at rt. TLC (tol/EtOAc, 80:20 or cHex/EtOAc, 70:30) showed the complete disappearance of the starting material and the presence of a less polar product. The suspension was filtered over a pad of Celite (DCM) and the solution was evaporated under reduced pressure. Flash column chromatography of the crude material (cHex/EtOAc, 80:20 to 50:50) gave donor 39 (7.2 g, 94%) as a white foam. The latter had $R_f = 0.4$ (cHex/EtOAc, 70:30). $^1$H NMR (400 MHz, CDCl₃) $\delta$ 7.54–7.08 (m, 48H, HAr), 6.90 (d, $J = 9.0$ Hz, 1H, HNHCO), 6.82 (d, $J = 8.0$ Hz, 2H, HAr), 6.44 (brs, 1H, H-1D), 5.61 (s, 1H, HBzl), 5.55 (dd, $J = 3.0$, 2.0 Hz, 1H, H-2A), 5.25 (d, $J = 3.2$ Hz, 1H, H-1E), 5.12 (dd, $J = 3.3$, 1.9 Hz, 1H, H-2C), 5.04–4.57 (m, 16H, H-1A, H-1B, H-1C, 13HBn), 4.53–4.42 (m, 3H, H-2D, 2HBn), 4.39 (dd, $J = 10.6$, 5.0 Hz, 1H, H-6AD), 4.36 (d, $J = 12.0$ Hz, 1H, HBn), 4.25 (dd, $J = 9.8$, 3.0 Hz, 1H, H-3A), 4.18 (tapp, $J = 9.5$ Hz, 1H, H-3D), 4.14–3.45 (m, 18H, H-5D, H-3E, H-2B, H-3C, H-5E, H-5C, H-3B, H-4E, H-5A, H-6bD, H-2E, H-4A, H-4B, H-4D, H-5B, H-5D, H-6aE, H-6bE), 3.31 (tapp, $J = 9.5$ Hz, 1H, H-4C), 2.62–2.37 (m, 4H, CH₂Lev), 2.09 (s, 3H, OCOCCH₃), 2.08 (s, 3H, CH₃Lev), 1.29 (d, $J = 6.2$ Hz, 3H, H-6A), 1.26 (d, $J = 6.2$ Hz, 3H, H-6B), 0.78 (d, $J = 6.2$ Hz, 3H, H-6C). $^{13}$C NMR (100 MHz, CDCl₃) $\delta$ 206.3 (COLev), 171.8 (CO₂Lev), 170.1 (OCOCH₃), 162.4 (CONH), 142.9 (OCNPhCF₃), 138.9–136.8 (10CAr), 129.4–126.6 (49CHAr), 125.0 (OCNPhCF₃), 119.4 (CHAr), 102.4 (CBzl), 101.1 (C-1B, $J_{C,H} = 170.7$ Hz), 99.3 (C-1A, $J_{C,H} = 173.8$ Hz), 98.0 (C-1C, $J_{C,H} = 173.5$ Hz), 93.9 (C-1D, $J_{C,H} = 170.2$ Hz), 93.0 (C-1E, $J_{C,H} = 170.2$ Hz), 92.1 (CCl₃), 82.3 (C-3E), 80.4 (C-4B), 80.1 (C-4C), 79.9 (C-4A), 79.7 (C-4D), 79.6 (C-2E), 79.4 (C-3B), 77.9 (C-4E), 77.4 (C-3C), 76.3 (CH₂Bn), 75.7 (2C, C-2B, CH₂Bn), 75.5 (2C, CH₂Bn), 75.3 (CH₂Bn), 75.1 (2C, CH₂Bn), 74.9 (C-3D), 73.6 (CH₂Bn), 72.9 (CH₂Bn), 72.5 (C-2C), 72.4 (C-3A), 72.2 (CH₂Bn), 70.4 (C-5E), 69.1 (C-5B), 68.8 (C-5A), 68.6 (C-6D), 68.4 (C-6E), 68.5 (C-5C), 68.2 (C-2A), 65.6 (C-5D), 54.9 (C-2D), 38.1 (CH₂Lev), 29.9 (CH₃Lev), 28.3 (CH₂Lev), 21.2 (OCOCH₃), 18.1 (C-6A), 18.0 (C-6B), 17.5 (C-6C). HRMS (ESI⁺): *m/z* 2060.7185 (calcd for C₁₁₀H₁₂₀Cl₃F₃N₃O₂₆Na [M + NH₄]⁺: *m/z* 2060.7178).

### Allyl (2,3,4,6-tetra-O-benzyl-α-D-glucopyranosyl)-(1 → 3)-(4-O-benzyl-2-O-levulinoyl-α-L-rhamnopyranosyl)-(1 → 2)-(3,4-di-O-benzyl-α-L-rhamnopyranosyl)-(1 → 3)-(4,6-O-benzylidene-2-deoxy-2-trichloroacetamido-β-D-glucopyranosyl)-(1 → 2)-[(2,3,4,6-tetra-O-benzyl-α-D-glucopyranosyl)-(1 → 3)]-(4-O-benzyl-α-L-rhamnopyranosyl)-(1 → 2)-(3,4-di-O-benzyl-α-L-rhamnopyranosyl)-(1 → 3)-(2-O-acetyl-4-O-benzyl-α-L-rhamnopyranosyl)-(1 → 3)-4,6-O-benzylidene-2-deoxy-2-trichloroacetamido-β-D-glucopyranoside (40)

To a solution of the glycosyl donor 39 (146 mg, 71 µmol, 1.30 equiv.) and glycosyl acceptor¹⁷ 37 (100 mg, 55 µmol, 1.0 equiv.)

in anhyd. toluene (0.6 mL) was added activated 4 Å MS (30 mg) and the suspension was stirred at rt under an argon atmosphere for 15 min, then for 10 min at -50 °C. TMSOTf (2 µL, 11 µmol, 0.2 equiv.) was added rapidly at -50 °C. The reaction mixture was stirred at -40 °C for 30 min, at which time a TLC (cHex/EtOAc, 70:30) follow-up indicated the consumption of the glycosyl acceptor and the presence of a major more polar product. Et₃N was added at -40 °C and the suspension was stirred for another 10 min. Solids were filtered over a pad of Celite and washed generously with DCM. The combined filtrates were concentrated to dryness and the residue was purified by flash column chromatography (cHex/EtOAc, 100:0 to 50:50) to give decasaccharide 40 (184 mg, 91%) as a white foam. The latter had $R_f = 0.3$ (cHex/EtOAc, 70:30). $^1$H NMR (400 MHz, CDCl₃) $\delta$ 7.50–7.02 (m, 91H, 90 HAr, HNHCO), 6.94 (d, $J = 9.0$ Hz, 1H, HNHCO), 5.94–5.83 (m, 1H, CH=CH₂), 5.56 (s, 1H, HBzl), 5.53 (dd, $J = 3.0$, 1.9 Hz, 1H, H-2A), 5.33–5.20 (m, 4H, HBzl, H-1E, CH=CH₂), 5.19–5.14 (m, 2H, H-1E, H-2C), 5.12 (d, $J = 8.2$ Hz, 1H, H-1D), 5.09 (brs, 1H, H-1B), 5.08–5.04 (m, 3H, H-2C, 2HBn), 5.01 (brs, 1H, H-1A), 5.01–4.95 (m, 2H, 2HBn), 4.95 (brs, 1H, H-1B'), 4.94–4.78 (m, 9H, H-1A, H-1C, 7HBn), 4.78–4.46 (m, 20H, H-1C, H-1D, H-3D, 17HBn), 4.46–4.28 (m, 6H, H-6aD, CH₂All, 4HBn), 4.23 (dd, $J = 9.7$, 2.6 Hz, 1H, H-3A), 4.19–3.93 (m, 12H, H-3E, CH₂All, H-2B, H-5E, H-3B, H-3E', H-3B', H-5E', H-2D', H-3C, H-2A, H-5C), 3.93–3.40 (m, 26H, H-2B', H-3C', H-5C', H-6aD', H-5A, H-5A', H-4E, H-4E', H-2E, H-2E', H-6bD, H-3A, H-5B, H-5B', H-4D, H-5D, H-6aE, H-6bE, H-6aE', H-6bE', H-4A, H-4A', H-4B, H-4B', H-2D), 3.36 (tapp, $J = 9.4$ Hz, 1H, H-4D'), 3.27 (tapp, $J = 9.5$ Hz, 1H, H-4C), 3.25 (tapp, $J = 9.5$ Hz, 1H, H-4C'), 3.13 (tapp, $J = 10.0$ Hz, 1H, H-6bD), 2.99–2.83 (m, 2H, H-3D, H-5D'), 2.60–2.35 (m, 4H, CH₂Lev), 2.07 (s, 3H, CH₃Lev), 2.06, 2.05 (2s, 6H, HAc), 1.36–1.23 (m, 12H, H-6A, H-6A', H-6B, H-6B'), 0.73 (d, $J = 6.2$ Hz, 3H, H-6C'), 0.69 (d, $J = 6.2$ Hz, 3H, H-6C). $^{13}$C NMR (100 MHz, CDCl₃) $\delta$ 206.3 (COLev), 171.8 (CO₂Lev), 170.0, 169.6 (2C, COAc), 162.3, 162.0 (2C, COCl₃Ac), 139.0–137.1 (18C, CAr), 133.4 (CH=CH₂), 129.5–126.5 (90C, CHAr), 118.5 (CH=CH₂), 102.2, 101.8 (2C, CBzl), 101.4 (C-1B', $J_{C,H} = 171.4$ Hz), 101.2 (C-1D', $J_{C,H} = 164.6$ Hz), 100.8 (C-1B, $J_{C,H} = 176.9$ Hz), 100.6 (C-1A, $J_{C,H} = 170.7$ Hz), 99.4 (C-1A', $J_{C,H} = 173.8$ Hz), 98.4 (C-1D, $J_{C,H} = 167.0$ Hz), 97.7 (2C, C-1C', C-1C, $J_{C,H} = 173.0$ Hz), 94.7 (C-1E, $J_{C,H} = 168.2$ Hz), 93.1 (2C, C-1E', $J_{C,H} = 170.2$ Hz, CC13), 92.3 (CC13), 83.3 (C-3E), 82.3 (C-3E'), 80.7 (C-4C'), 80.6 (C-4B), 80.4 (C-4D), 80.3 (C-4B'), 80.1 (2C, C-4A, C-4C), 80.0 (C-4D'), 79.9 (C-4A'), 79.7 (C-2E'), 79.6 (C-3A), 79.3 (C-2E), 78.8 (C-4E), 78.5 (C-3C), 77.9 (C-4E'), 77.7 (C-3D), 77.4 (C-3C'), 76.9 (C-3B'), 76.4 (C-2B'), 76.3 (CH₂Bn), 76.1 (CH₂Bn), 75.7 (CH₂Bn), 75.5 (CH₂Bn), 75.2 (CH₂Bn), 75.1 (CH₂Bn), 75.0 (CH₂Bn), 74.7 (C-3B), 74.3 (C-2A), 74.0 (C-3D), 73.7 (CH₂Bn), 73.5 (CH₂Bn), 73.4(C-2B), 72.9 (CH₂Bn), 72.6 (C-2C'), 72.5 (C-3A'), 72.3 (C-2C), 72.2 (CH₂Bn), 71.0 (CH₂All), 70.4 (C-5E'), 70.2 (C-5E), 69.1 (C-5B'), 69.0 (C-5B), 68.9 (C-6D), 68.8 (C-5A), 68.5 (C-6E'), 68.3(C-2A'), 68.2 (C-6D'), 68.1(C-5C), 68.0 (C-6E), 67.9 (C-5C), 66.4 (C-5D), 66.1 (C-5D'), 60.5 (C-2D), 57.7 (C-2D'), 38.1 (CH₂Lev), 29.9 (CH₃Lev), 28.3 (CH₂Lev), 21.2 (OCOCH₃), 21.1 (OCOCH₃), 18.1–18.0 (4C, C-6A, C-6A', C-6B, C-6B'), 17.5 (C-6C'), 17.4 (C-6C). HRMS (ESI⁺): *m/z* 1854.1809 (calcd for C₂₀₂H₂₂₇Cl₆N₄O₄ [M + 2NH₄]⁺: *m/z* 1854.1814).
## 2-Azidoethyl (2,3,4,6-tetra-O-benzyl-α-D-glucopyranosyl)-(1 → 3)-(4-O-benzyl-2-O-levulinoyl-α-L-rhamnopyranosyl)-(1 → 2)-(3,4-di-O-benzyl-α-L-rhamnopyranosyl)-(1 → 3)-(2-O-acetyl-4-O-benzyl-α-L-rhamnopyranosyl)-(1 → 3)-4,6-O-benzylidene-2-deoxy-2-trichloroacetamido-β-D-glucopyranoside (41)

To a solution of 2-azidoethanol (68 µL, 897 µmol, 3.1 equiv.) and glycosyl donor 39 (600 mg, 293 µmol, 1.0 equiv.) in anhyd. toluene (2.9 mL) was added activated 4 Å MS (470 mg) and the suspension was stirred at rt under an argon atmosphere for 15 min, then for 10 min at -50 °C. TMSOTf (21 µL, 0.12 mmol, 0.2 equiv.) was added rapidly at -50 °C. The reaction mixture was stirred at -40 °C for 30 min, at which time a TLC (cHex/EtOAc, 70:30) follow-up showed the consumption of the donor. Et<sub>3</sub>N was added at this temperature. After stirring for 10 min, solids were filtered over a pad of Celite and washed generously with DCM. The combined filtrates were concentrated to dryness and the residue was purified by flash column chromatography (cHex/EtOAc, 90:10 to 50:50) to give the azidoethyl pentasaccharide 41 (461 mg, 81%) as a white foam. The latter had R<sub>f</sub> = 0.25 (cHex/EtOAc, 70:30). <sup>1</sup>H NMR (400 MHz, CDCl<sub>3</sub>) δ 7.52-7.06 (m, 46H, H<sub>Ar</sub>, H<sub>NHCO</sub>), 5.56 (s, 1H, H<sub>Bzl</sub>), 5.54 (brs, 1H, H-2<sub>A</sub>), 5.24 (d, J = 3.1 Hz, 1H, H-1<sub>E</sub>), 5.18 (d, J = 8.5 Hz, 1H, H-1<sub>D</sub>), 5.17 (dd, J = 3.3, 1.9 Hz, 1H, H-2<sub>C</sub>), 5.03-4.84 (m, 8H, H-1<sub>A</sub>, H-1<sub>B</sub>, H-1<sub>C</sub>, 5H<sub>Bn</sub>), 4.78 (d, J = 13.1 Hz, 1H, H<sub>Bn</sub>), 4.67 (d, J = 13.1 Hz, 1H, H<sub>Bn</sub>), 4.70-4.32 (m, 11H, H-3<sub>D</sub>, H-6<sub>B</sub>, 9H<sub>Bn</sub>), 4.24 (dd, J = 9.7, 2.6 Hz, 1H, H-3<sub>A</sub>), 4.15-3.92 (m, 6H, H-2<sub>B</sub>, H-3<sub>C</sub>, OCH<sub>2</sub>CH<sub>2</sub>N<sub>3</sub>, H-3<sub>E</sub>, H-5<sub>C</sub>, H-5<sub>E</sub>), 3.87-3.70 (m, 5H, H-3<sub>B</sub>, H-4<sub>E</sub>, H-5<sub>A</sub>, H-6<sub>A</sub><sub>D</sub>, OCH<sub>2</sub>CH<sub>2</sub>N<sub>3</sub>), 3.70-3.34 (m, 11H, H-2<sub>D</sub>, H-2<sub>E</sub>, H-4<sub>A</sub>, H-4<sub>B</sub>, H-4<sub>D</sub>, H-5<sub>B</sub>, H-5<sub>D</sub>, H-6<sub>A</sub><sub>E</sub>, H-6<sub>B</sub><sub>E</sub>, CH<sub>2</sub>N<sub>3</sub>), 3.29 (t<sub>app</sub>, J = 9.5 Hz, 1H, H-4<sub>C</sub>), 2.58-2.41 (m, 4H, CH<sub>2</sub>Lev), 2.08 (s, 3H, CH<sub>3</sub>Lev), 2.06 (s, 3H, OCOCH<sub>3</sub>), 1.28 (d, J = 6.2 Hz, 3H, H-6<sub>A</sub>), 1.25 (d, J = 6.2 Hz, 3H, H-6<sub>B</sub>), 0.73 (d, J = 6.2 Hz, 3H, H-6<sub>C</sub>). <sup>13</sup>C NMR (100 MHz, CDCl<sub>3</sub>) δ 206.2 (CO<sub>Lev</sub>), 171.8 (CO<sub>2Lev</sub>), 169.9 (OCOCH<sub>3</sub>), 162.3 (CONH), 138.9-137.1 (9C<sub>Ar</sub>), 129.2-126.5 (45CH<sub>Ar</sub>), 102.1 (C<sub>Bzl</sub>), 101.2 (C-1<sub>B</sub>, J<sub>C,H</sub> = 170.7 Hz), 99.1 (C-1<sub>D</sub>, J<sub>C,H</sub> = 168.9 Hz), 99.1 (C-1<sub>A</sub>, J<sub>C,H</sub> = 173.8 Hz), 97.6 (C-1<sub>C</sub>, J<sub>C,H</sub> = 173.5 Hz), 92.9 (C-1<sub>E</sub>, J<sub>C,H</sub> = 170.2 Hz), 92.2 (CCl<sub>3</sub>), 82.2 (C-3<sub>E</sub>), 80.4 (C-4<sub>D</sub>), 80.3 (C-4<sub>B</sub>), 80.0 (C-4<sub>C</sub>), 79.9 (C-4<sub>A</sub>), 79.5 (C-2<sub>E</sub>), 79.4 (C-3<sub>B</sub>), 78.5 (C-3<sub>C</sub>), 77.8 (C-4<sub>E</sub>), 76.4 (CH<sub>2</sub>Bn), 75.7 (CH<sub>2</sub>Bn), 75.4 (CH<sub>2</sub>Bn), 75.3 (C-2<sub>B</sub>), 75.2 (CH<sub>2</sub>Bn), 75.1 (CH<sub>2</sub>Bn), 73.8 (C-3<sub>D</sub>), 73.5 (CH<sub>2</sub>Bn), 72.9 (CH<sub>2</sub>Bn), 72.3 (C-3<sub>A</sub>), 72.2 (2C, C-2<sub>C</sub>, CH<sub>2</sub>Bn), 70.4 (C-5<sub>E</sub>), 69.1 (C-5<sub>B</sub>), 69.0 (OCH<sub>2</sub>CH<sub>2</sub>N<sub>3</sub>), 68.7 (2C, C-6<sub>D</sub>, C-5<sub>A</sub>), 68.4 (C-6<sub>E</sub>), 68.1 (2C, C-2<sub>A</sub>, C-5<sub>C</sub>), 66.4 (C-5<sub>D</sub>), 60.4 (C-2<sub>D</sub>), 50.9 (OCH<sub>2</sub>CH<sub>2</sub>N<sub>3</sub>), 38.0 (CH<sub>2</sub>Lev), 29.8 (CH<sub>3</sub>Lev), 28.2 (CH<sub>2</sub>Lev), 21.1 (OCOCH<sub>3</sub>), 18.0 (2C, C-6<sub>A</sub>, C-6<sub>B</sub>), 17.3 (C-6<sub>C</sub>). HRMS (ESI<sup>+</sup>): *m/z* 1865.6378 (*calcd* for C<sub>99</sub>H<sub>109</sub>Cl<sub>3</sub>N<sub>4</sub>O<sub>24</sub>Na [M + Na]<sup>+</sup>: *m/z* 1865.6395).

## 2-Azidoethyl (2,3,4,6-tetra-O-benzyl-α-D-glucopyranosyl)-(1 → 3)-(4-O-benzyl-α-L-rhamnopyranosyl)-(1 → 2)-(3,4-di-O-benzyl-α-L-rhamnopyranosyl)-(1 → 3)-(2-O-acetyl-4-O-benzyl-α-L-rhamnopyranosyl)-(1 → 3)-4,6-O-benzylidene-2-deoxy-2-trichloroacetamido-β-D-glucopyranoside (42)

Hydrazine hydrate (34 µL, 700 µmol, 2 equiv.) was added to a solution of the fully protected 41 (682 mg, 350 µmol) in pyri-

dine-AcOH (3:2 v/v, 7 mL) and the resulting solution was stirred at rt for 30 min. TLC (cHex/EtOAc, 70:30) showed the complete conversion of the starting material into a less polar compound. Water (100 mL) and EtOAc (100 mL) were added and the phases were separated. The aq. layer was extracted with EtOAc and the combined organic layers were washed with sat. aq. NaHCO<sub>3</sub> and brine, dried on Na<sub>2</sub>SO<sub>4</sub>, filtered, and concentrated under reduced pressure. Flash chromatography of the crude (tol/EtOAc, 90:10 to 70:30) gave alcohol 42 (589 mg, 92%) as a white foam. The latter had R<sub>f</sub> = 0.3 (cHex/EtOAc, 70:30). <sup>1</sup>H NMR (400 MHz, CDCl<sub>3</sub>) δ 7.52-7.06 (m, 46H, H<sub>Ar</sub>, H<sub>NHCO</sub>), 5.56 (s, 1H, H<sub>Bzl</sub>), 5.54 (brs, 1H, H-2<sub>A</sub>), 5.24 (d, J = 3.1 Hz, 1H, H-1<sub>E</sub>), 5.18 (d, J = 8.5 Hz, 1H, H-1<sub>D</sub>), 5.17 (dd, J = 3.3, 1.9 Hz, 1H, H-2<sub>C</sub>), 5.13 (brs, 1H, H-1<sub>A</sub>), 4.97 (d, J = 1.9 Hz, 1H, H-1<sub>B</sub>), 4.99-4.81 (m, 7H, H-1<sub>E</sub>, H-1<sub>C</sub>, 5H<sub>Bn</sub>), 4.78-4.28 (m, 13H, H-3<sub>D</sub>, H-6<sub>B</sub><sub>D</sub>, 11H<sub>Bn</sub>), 4.09-3.91 (m, 8H, H-3<sub>E</sub>, H-2<sub>A</sub>, OCH<sub>2</sub>CH<sub>2</sub>N<sub>3</sub>, H-3<sub>A</sub>, H-2<sub>B</sub>, H-3<sub>C</sub>, H-5<sub>C</sub>, H-5<sub>E</sub>), 3.87-3.56 (m, 9H, H-5<sub>A</sub>, H-4<sub>A</sub>, H-6<sub>A</sub><sub>D</sub>, H-4<sub>E</sub>, OCH<sub>2</sub>CH<sub>2</sub>N<sub>3</sub>, H-5<sub>B</sub>, H-4<sub>B</sub>, H-5<sub>D</sub>, H-2<sub>E</sub>), 3.54-3.33 (m, 7H, H-3<sub>B</sub>, H-4<sub>D</sub>, H-2<sub>D</sub>, H-6<sub>A</sub><sub>E</sub>, H-6<sub>B</sub><sub>E</sub>, CH<sub>2</sub>N<sub>3</sub>), 3.28 (t<sub>app</sub>, J = 9.5 Hz, 1H, H-4<sub>C</sub>), 1.68 (brs, 1H, OH), 1.26 (d, J = 6.2 Hz, 3H, H-6<sub>A</sub>), 1.25 (d, J = 6.2 Hz, 3H, H-6<sub>B</sub>), 0.73 (d, J = 6.2 Hz, 3H, H-6<sub>C</sub>). <sup>13</sup>C NMR (100 MHz, CDCl<sub>3</sub>) δ 169.9 (OCOCH<sub>3</sub>), 162.3 (CONH), 138.8-137.1 (9C<sub>Ar</sub>), 129.2-127.6 (45CH<sub>Ar</sub>), 102.1 (C<sub>Bzl</sub>), 101.4 (C-1<sub>B</sub>, J<sub>C,H</sub> = 170.7 Hz), 100.8 (C-1<sub>A</sub>, J<sub>C,H</sub> = 173.8 Hz), 99.1 (C-1<sub>D</sub>, J<sub>C,H</sub> = 168.9 Hz), 97.5 (C-1<sub>C</sub>, J<sub>C,H</sub> = 173.5 Hz), 94.0 (C-1<sub>E</sub>, J<sub>C,H</sub> = 170.2 Hz), 92.2 (CCl<sub>3</sub>), 82.5 (C-3<sub>E</sub>), 80.4 (C-4<sub>D</sub>), 80.2 (C-4<sub>B</sub>), 80.0 (C-4<sub>C</sub>), 79.7 (C-4<sub>A</sub>), 79.3 (C-3<sub>B</sub>), 79.0 (C-2<sub>E</sub>), 78.4 (C-3<sub>C</sub>), 77.8 (C-4<sub>E</sub>), 76.5 (C-2<sub>B</sub>), 75.7 (2C, CH<sub>2</sub>Bn), 75.3 (CH<sub>2</sub>Bn), 75.2 (CH<sub>2</sub>Bn), 75.0 (2C, C-3<sub>A</sub>, CH<sub>2</sub>Bn), 74.5 (CH<sub>2</sub>Bn), 73.8 (C-3<sub>D</sub>), 73.5 (CH<sub>2</sub>Bn), 72.4 (CH<sub>2</sub>Bn), 72.2 (C-2<sub>C</sub>), 70.8 (C-5<sub>E</sub>), 69.1 (C-5<sub>B</sub>), 69.0 (OCH<sub>2</sub>CH<sub>2</sub>N<sub>3</sub>), 68.7 (C-6<sub>D</sub>), 68.1 (C-5<sub>C</sub>), 68.0 (C-6<sub>E</sub>), 67.9 (C-5<sub>A</sub>), 67.4 (C-2<sub>A</sub>), 66.4 (C-5<sub>D</sub>), 60.4 (C-2<sub>D</sub>), 50.9 (OCH<sub>2</sub>CH<sub>2</sub>N<sub>3</sub>), 21.1 (OCOCH<sub>3</sub>), 18.0 (2C, C-6<sub>A</sub>, C-6<sub>B</sub>), 17.3 (C-6<sub>C</sub>). HRMS (ESI<sup>+</sup>): *m/z* 1865.6378 (*calcd* for C<sub>99</sub>H<sub>109</sub>Cl<sub>3</sub>N<sub>4</sub>O<sub>24</sub>Na [M + Na]<sup>+</sup>: *m/z* 1865.6395).

## Azidoethyl (2,3,4,6-tetra-O-benzyl-α-D-glucopyranosyl)-(1 → 3)-(4-O-benzyl-2-O-levulinoyl-α-L-rhamnopyranosyl)-(1 → 2)-(3,4-di-O-benzyl-α-L-rhamnopyranosyl)-(1 → 3)-(2-O-acetyl-4-O-benzyl-α-L-rhamnopyranosyl)-(1 → 3)-(4,6-O-benzylidene-2-deoxy-2-trichloroacetamido-β-D-glucopyranosyl)-(1 → 2)-[[2,3,4,6-tetra-O-benzyl-α-D-glucopyranosyl)-(1 → 3]-(4-O-benzyl-α-L-rhamnopyranosyl)-(1 → 2)-(3,4-di-O-benzyl-α-L-rhamnopyranosyl)-(1 → 3)-(2-O-acetyl-4-O-benzyl-α-L-rhamnopyranosyl)-(1 → 3)-(4,6-O-benzylidene-2-deoxy-2-trichloroacetamido-β-D-glucopyranoside (43)

To a solution of donor 39 (1.26 g, 616 µmol, 1.3 equiv.) and acceptor 42 (876 mg, 475 µmol) in anhyd. toluene (5.0 mL) was added activated 4 Å MS (282 mg). The suspension was stirred at rt under an argon atmosphere for 15 min, then for 10 min at -50 °C. TMSOTf (18 µL, 99 µmol, 0.21 equiv.) was added rapidly at -50 °C. The reaction mixture was stirred at -40 °C for 30 min, at which time a TLC (cHex/EtOAc, 70:30) follow-up showed the consumption of the acceptor. Et<sub>3</sub>N was added at -40 °C and the suspension was stirred for another 10 min. Solids were filtered over a pad of Celite and washed generously with DCM. The combined filtrates were concentrated to
<figure><img src="image_27.png" /></figure>

dryness and the residue was purified by flash column chromatography (cHex/EtOAc, 100:0 to 50:50) to deliver decasaccharide **43** (1.6 g, 91%) as a white foam. The latter had $R_f = 0.3$ (cHex/EtOAc, 70:30). ¹H NMR (400 MHz, CDCl₃) $\delta$ 7.50–7.02 (m, 91H, 90 HAr, HNHCOCl), 6.92 (d, *J* = 9.0 Hz, 1H, HNHCO), 5.56 (s, 1H, HBzl), 5.53 (dd, *J* = 3.0, 1.9 Hz, 1H, H-2A'), 5.30 (s, 1H, HBzl), 5.22 (d, *J* = 3.1 Hz, 1H, H-1E'), 5.20–5.14 (m, 3H, H-1D, H-1E, H-2C), 5.11–5.04 (m, 4H, H-2C', H-1B, 2HBn), 5.03–4.27 (m, 38H, H-1B', H-1A', H-1A, H-1C, H-1C', H-1D, H-3D, H-6bD, 30HBn), 4.23 (dd, *J* = 9.7, 2.6 Hz, 1H, H-3A'), 4.19–3.93 (m, 12H, H-3E, H-2B, H-5E, H-3B, OCH₂CH₂N₂, H-3E', H-3B', H-5E', H-2D', H-3C, H-2A, H-5C), 3.93–3.33 (m, 30H, H-2B', H-3C', H-5C', H-6aD', H-5A, H-5A', H-4E, H-4E', H-2E, H-2E', H-6aD, OCH₂CH₂N₃, H-3A, H-5B, H-5B', H-4D, H-5D, H-6aE, H-6bE, H-6aE', H-6bE', CH₂N₃, H-4A, H-4A', H-4B, H-4B', H-2D, H-4D'), 3.27 (tapp, *J* = 9.5 Hz, 1H, H-4C), 3.25 (tapp, *J* = 9.5 Hz, 1H, H-4C'), 3.13 (tapp, *J* = 10.0 Hz, 1H, H-6bD), 2.99–2.83 (m, 2H, H-3D', H-5D'), 2.60–2.35 (m, 4H, CH₂Lev), 2.07 (s, 3H, CH₃Lev), 2.06, 2.05 (2s, 6H, HAc), 1.36–1.23 (m, 12H, H-6A, H-6A', H-6B, H-6B'), 0.73 (d, *J* = 6.2 Hz, 3H, H-6C'), 0.69 (d, *J* = 6.2 Hz, 3H, H-6C). ¹³C NMR (100 MHz, CDCl₃) $\delta$ 206.3 (COLev), 171.8 (CO₂Lev), 170.0, 169.6 (2C, COAc), 162.5, 162.0 (2C, COCl₃Ac), 139.0–137.1 (18C, CAr), 129.5–126.5 (90C, CHAr), 102.2, 101.8 (2C, CBzl), 101.5 (C-1B', JC,H = 171.4 Hz), 101.2 (C-1D', JC,H = 164.6 Hz), 100.8 (C-1B, JC,H = 176.9 Hz), 100.6 (C-1A, JC,H = 170.7 Hz), 99.4 (C-1A', JC,H = 173.8 Hz), 99.2 (C-1D, JC,H = 165.0 Hz), 97.7 (2C, C-1C', C-1C, JC,H = 173.0 Hz), 94.6 (C-1E, JC,H = 168.2 Hz), 93.1 (2C, C-1E', JC,H = 170.2 Hz, CC13), 92.3 (CC13), 83.3 (C-3E), 82.3 (C-3E'), 80.7 (C-4C'), 80.6 (C-4B), 80.3 (2C, C-4D, C-4B'), 80.1 (C-4A), 80.0 (2C, C-4C, C-4D'), 79.9 (C-4A'), 79.7 (C-2E'), 79.6 (C-3A), 79.3 (C-2E), 78.8 (C-4E), 78.6 (C-3C), 77.9 (C-4E'), 77.7 (C-3D'), 77.4 (C-3C'), 76.9 (C-3B'), 76.4 (C-2B'), 76.3 (CH₂Bn), 76.1 (CH₂Bn), 75.7 (CH₂Bn), 75.5 (CH₂Bn), 75.2 (CH₂Bn), 75.1 (CH₂Bn), 75.0 (CH₂Bn), 74.6 (C-3B), 74.4 (C-2A), 73.9 (C-3D), 73.7 (CH₂Bn), 73.5 (CH₂Bn), 73.4(C-2B), 72.9 (CH₂Bn), 72.6 (C-2C'), 72.5 (C-3A'), 72.3 (C-2C), 72.2 (CH₂Bn), 70.3 (C-5E'), 70.2 (C-5E), 69.1 (C-5B'), 69.0 (3C, OCH₂CH₂N₂, C-6D, C-5B), 68.8 (C-5A'), 68.5 (C-6E'), 68.3 (C-2A'), 68.2 (C-6D'), 68.1 (C-5C), 68.0 (C-6E'), 67.9 (C-5C'), 66.5 (C-5D), 66.1 (C-5D'), 60.5 (C-2D), 57.7 (C-2D'), 50.9 (OCH₂CH₂N₃), 38.1 (CH₂Lev), 29.9 (CH₃Lev), 28.3 (CH₂Lev), 21.2 (OCOCH₃), 21.1 (OCOCH₃), 18.1–18.0 (4C, C-6A, C-6A', C-6B, C-6B'), 17.5 (C-6C'), 17.4 (C-6C). HRMS (ESI⁺): *m/z* 1868.6818 (calcd for C₂₀₁H₂₂₇Cl₆N₇O₄₉ [M + 2NH₄]²⁺: *m/z* 1868.6820).

**Azidoethyl (2,3,4,6-tetra-O-benzyl-α-D-glucopyranosyl)-(1 → 3)-(4-O-benzyl-α-L-rhamnopyranosyl)-(1 → 2)-(3,4-di-O-benzyl-α-L-rhamnopyranosyl)-(1 → 3)-(4,6-O-benzylidene-2-deoxy-2-trichloroacetamido-β-D-glucopyranosyl)-(1 → 2)-[(2,3,4,6-tetra-O-benzyl-α-D-glucopyranosyl)-(1 → 3)]-(4-O-benzyl-α-L-rhamnopyranosyl)-(1 → 2)-(3,4-di-O-benzyl-α-L-rhamnopyranosyl)-(1 → 3)-(2-O-acetyl-4-O-benzyl-α-L-rhamnopyranosyl)-(1 → 3)-4,6-O-benzylidene-2-deoxy-2-trichloroacetamido-β-D-glucopyranoside (44)**

**Route 1.** Hydrazine hydrate (40 µL, 825 µmol, 2.2 equiv.) was added to decasaccharide **43** (1.4 g, 378 µmol) in pyridine/

AcOH (3:2 v/v, 7.6 mL) and the solution was stirred at rt for 1 h. TLC (cHex/EtOAc, 70:30) showed the complete conversion of the starting material into a closely migrating less polar compound. The mixture was partitioned between water (200 mL) and EtOAc (200 mL). The aq. layer was extracted with EtOAc (100 mL twice). The combined organic layers were washed with sat. aq. NaHCO₃ (300 mL) and brine (300 mL), dried on Na₂SO₄, filtered, and concentrated under reduced pressure. Flash column chromatography of the crude (cHex/EtOAc, 90:10 to 50:50) gave alcohol **44** (1.24 g, 91%) as a white foam. The latter had $R_f = 0.3$ (cHex/EtOAc, 70:30). ¹H NMR (400 MHz, CDCl₃) $\delta$ 7.50–7.02 (m, 91H, 90 HAr, HNHCO), 6.92 (d, *J* = 9.0 Hz, 1H, HNHCO), 5.55 (s, 1H, HBzl), 5.31 (s, 1H, HBzl), 5.20–5.14 (m, 3H, H-1D, H-1E, H-2C), 5.11–5.04 (m, 5H, H-1A', H-2C', H-1B, 2HBn), 5.03 (brs, 1H, H-1A), 5.01–4.23 (m, 38H, H-1B', H-1E', H-1C, H-1C', H-1D', H-3D, H-6bD, H-3A', 30HBn), 4.18–3.55 (m, 32H, H-2A', H-3E, H-2B, H-5E, OCH₂CH₂N₂, H-3E', H-3B', H-5E', H-2D', H-3C, H-2A, H-5C, H-2B', H-3C', H-5C', H-6aD', H-5A, H-5A', H-4E, H-4E', H-2E, H-2E', H-6aD, OCH₂CH₂N₂, H-3A, H-5B, H-5B', H-4B', H-3B), 3.52–3.32 (m, 12H, H-4D, H-5D, H-6aE, H-6bE, H-6aE', H-6bE', CH₂N₃, H-4A, H-4A', H-4B, H-2D, H-4D'), 3.27 (tapp, *J* = 9.5 Hz, 1H, H-4C), 3.25 (tapp, *J* = 9.5 Hz, 1H, H-4C'), 3.13 (tapp, *J* = 10.0 Hz, 1H, H-6bD), 2.99–2.83 (m, 2H, H-3D', H-5D'), 2.06, 2.05 (2s, 6H, HAc), 1.35 (d, *J* = 6.2 Hz, 3H, H-6A'), 1.29–1.23 (m, 9H, H-6A, H-6B, H-6B'), 0.72 (d, *J* = 6.2 Hz, 3H, H-6C'), 0.69 (d, *J* = 6.2 Hz, 3H, H-6C). ¹³C NMR (100 MHz, CDCl₃) $\delta$ 170.0, 169.6 (2C, COAc), 162.5, 162.0 (2C, COCl₃Ac), 139.0–137.1 (18C, CAr-C), 129.5–126.5 (90C, CHAr), 102.2, 101.8 (2C, CBzl), 101.5 (C-1B', JC,H = 170.7 Hz), 101.3 (C-1D', JC,H = 164.6 Hz), 101.2 (C-1A', JC,H = 173.8 Hz), 100.8 (2C, C-1B, JC,H = 176.9 Hz, C-1A, JC,H = 170.7 Hz), 99.2 (C-1D, JC,H = 165.0 Hz), 97.7 (2C, C-1C', C-1C, JC,H = 173.0 Hz), 94.7 (C-1E, JC,H = 168.2 Hz), 94.2 (C-1E', JC,H = 170.2 Hz), 93.1 (CC13), 92.3 (CC13), 83.3 (C-3E), 82.6 (C-3E'), 80.8 (C-4C'), 80.6 (C-4B), 80.3 (2C, C-4D, C-4B'), 80.1 (C-4A), 80.0 (2C, C-4C, C-4D'), 79.9 (C-4A'), 79.6 (C-2E'), 79.5 (C-3A), 79.1 (C-2E), 78.8 (C-4E), 78.6 (C-3C), 77.9 (C-4E'), 77.6 (C-3D'), 77.4 (C-3C'), 76.8 (C-3B'), 76.7 (C-2B'), 76.3 (CH₂Bn), 76.1 (CH₂Bn), 75.7 (CH₂Bn), 75.5 (CH₂Bn), 75.2 (CH₂Bn), 75.1 (CH₂Bn), 75.0 (CH₂Bn), 74.6 (C-3B), 74.4 (C-2A), 73.9 (C-3D), 73.7 (CH₂Bn), 73.5 (CH₂Bn), 73.4(C-2B), 72.7 (C-2C'), 72.5 (CH₂Bn), 72.3 (C-3A'), 72.3 (C-2C), 72.2 (CH₂Bn), 70.8 (C-5E'), 70.2 (C-5E), 69.1 (C-5B'), 69.0 (3C, OCH₂CH₂N₃, C-5C, C-5B), 68.8 (C-6D), 68.3 (C-6D'), 68.2 (C-5C), 68.0 (C-6E', C-6E), 67.9 (C-5A'), 67.6 (C-2A'), 66.5 (C-5D), 66.1 (C-5D'), 60.5 (C-2D), 57.7 (C-2D'), 50.9 (OCH₂CH₂N₂), 21.2 (OCOCH₃), 21.1 (OCOCH₃), 18.1–18.0 (4C, C-6A, C-6A', C-6B, C-6B'), 17.5 (C-6C'), 17.4 (C-6C).

**2-Aminoethyl α-D-glucopyranosyl-(1 → 3)-α-L-rhamnopyranosyl-(1 → 2)-α-L-rhamnopyranosyl-(1 → 3)-(2-O-acetyl-α-L-rhamnopyranosyl)-(1 → 3)-2-acetamido-2-deoxy-β-D-glucopyranoside (45)**

A solution of alcohol **42** (255 mg, 138 µmol) in tBuOH/DCM/H₂O (7:2:1, 25 mL) was degassed repeatedly. Next, 20% Pd(OH)₂/C (255 mg) was added and the suspension was stirred vigorously overnight under a hydrogen atmosphere. Analytical RP-HPLC indicated the presence of the desired **45** as the only
<figure><img src="image_28.png" /></figure>

sugar detected. The suspension was centrifuged and the supernatant was passed through a PVDF membrane (0.2 µm, 25 mm). The residue was suspended in tBuOH/H₂O (1:4, 5.0 mL) and centrifuged (5000 min⁻¹). The supernatant was passed through a PVDF membrane (0.2 µm). The procedure was repeated three times. The combined filtrates were freeze-dried and the residue was filtered through a Sep-Pak C18 cartridge eluting first with 0.08% ac. TFA then with 20% MeCN in 0.08% ac. TFA. The suitable fractions were pooled, freeze-dried and the residue was purified by RP-HPLC to give penta-saccharide 45 (85 mg, 70%) as a white powder. The linker-equipped 45 had RP-HPLC (λ = 215 nm): tR = 10.46 min ¹H NMR (400 MHz, D₂O) δ 5.12 (brs, 1H, H-1B), 5.09 (d, *J* = 3.9 Hz, H-1E), 5.02–4.97 (m, 2H, H-2C, H-1A), 4.87 (brs, 1H, H-1C), 4.55 (d, *J* = 8.5 Hz, 1H, H-1D), 4.26 (tapp, *J* = 2.4 Hz, 1H, H-2A), 4.12–3.99 (m, 3H, H-5C, H-2B, OCH₂CH₂NH₂), 3.98–3.86 (m, 4H, H-5E, H-3C, H-6aD, OCH₂CH₂NH₂), 3.86–3.68 (m, 8H, H-3A, H-2D, H-3E, H-6aE, H-6bE, H-6bD, H-5A, H-3B), 3.63–3.41 (m, 9H, H-2E, H-4C, H-5B, H-4A, H-3D, H-4D, H-4E, H-4B, H-5D), 3.26–3.13 (m, 2H, CH₂NH₂), 2.16 (s, 3H, HAC), 2.05 (s, 3H, HNAC), 1.30–1.21 (m, 9H, H-6A, H-6B, H-6C). ¹³C NMR (100 MHz, D₂O) δ 177.4 (CONAC), 175.6 (COAC), 104.6 (C-1A, JC,H = 173.4 Hz), 103.7 (C-1B, JC,H = 172.4 Hz), 103.1 (C-1D, JC,H = 162.7 Hz), 101.2 (C-1C, JC,H = 173.5 Hz), 98.0 (C-1E, JC,H = 170.3 Hz), 84.9 (C-3D), 80.8 (C-2B), 78.8 (C-3C), 78.6 (C-5D), 77.9 (C-3A), 75.6 (C-3E), 74.9 (C-2C), 74.5 (C-4B), 74.3 (C-2E, C-4C), 74.1 (C-5E), 72.9 (C-4A), 72.6 (C-3B), 72.1 (2C, C-5A, C-5B), 71.9 (C-4E), 71.6 (C-5C), 70.9 (C-4D), 69.3 (C-2A), 68.3 (OCH₂CH₂CH₂NH₂), 63.3 (C-6D), 63.0 (C-6E), 57.7 (C-2D), 42.1 (CH₂NH₂), 24.9 (CNAC), 22.9 (CAC), 19.4 (C-6A), 19.3 (C-6B), 18.9 (C-6C). HRMS (ESI⁺): *m/z* 930.3611 (calcd for C₃₆H₆₂N₂O₂₄Na [M + Na]⁺: *m/z* 930.3624).

2-Aminoethyl α-D-glucopyranosyl-(1 → 3)-α-L-rhamnopyranosyl-(1 → 2)-α-L-rhamnopyranosyl-(1 → 3)-(2-O-acetyl-α-L-rhamnopyranosyl)-(1 → 3)-(2-acetamido-2-deoxy-β-D-glucopyranosyl)-(1 → 2)-[α-D-glucopyranosyl-(1 → 3)]-α-L-rhamnopyranosyl-(1 → 2)-α-L-rhamnopyranosyl-(1 → 3)-(2-O-acetyl-α-L-rhamnopyranosyl)-(1 → 3)-(2-acetamido-2-deoxy-β-D-glucopyranosyl)-(1 → 2)-[α-D-glucopyranosyl-(1 → 3)]-α-L-rhamnopyranosyl-(1 → 2)-α-L-rhamnopyranosyl-(1 → 3)-(2-O-acetyl-α-L-rhamnopyranosyl)-(1 → 3)-2-acetamido-2-deoxy-β-D-glucopyranoside (46)

Alcohol 44 (50 mg, 14 µmol) was dissolved in tBuOH/DCM/H₂O (7:2:1, 5 mL) and the solution was degassed repeatedly. Then, 20 wt% Pd(OH)₂/C (50 mg) was added and the suspension was stirred vigorously overnight under a hydrogen atmosphere. After 12 h, analytical RP-HPLC indicated the presence of several products corresponding to diversely N-chloroacetylated analogues of the desired 46. Et₃N (4 equiv.) was added, the suspension was centrifuged and the supernatant was passed through a PVDF membrane (0.2 µm, 25 mm). The residue was suspended in tBuOH/H₂O (1:4, 5.0 mL) and centrifuged (5000 min⁻¹). The supernatant was passed through a PVDF membrane (0.2 µm). The procedure was repeated three times. The combined filtrates were freeze-

dried. The residue obtained was dissolved in tBuOH/DCM/H₂O (7:2:1, 5.0 mL) and the solution was degassed repeatedly. Then, 20% Pd(OH)₂/C (100 mg) was added and the suspension was stirred vigorously overnight under a hydrogen atmosphere for another 2 days with analytical RP-HPLC follow-up. The suspension was centrifuged and the supernatant was passed through a PVDF membrane (0.2 µm). The residue was suspended in tBuOH/H₂O (1:4, 5.0 mL) and centrifuged (5000 min⁻¹). The supernatant was passed through a PVDF membrane (0.2 µm). This was repeated three times. The combined filtrates were concentrated by freeze-drying. The residue was dissolved in 0.5 mL H₂O and passed through a Sep-Pak C18 cartridge, eluting first with 0.08% ac. TFA then with 20% CH₃CN in 0.08% ac. TFA. Suitable fractions were pooled, freeze-dried and the residue was purified by RP-HPLC to give decasaccharide 46 (13 mg, 52%) as a white powder. The linker-equipped 46 had RP-HPLC (λ = 215 nm): tR = 11.73 min ¹H NMR (400 MHz, D₂O) δ 5.16 (d, *J* = 3.6 Hz, 1H, H-1E), 5.12 (brs, 1H, H-1B), 5.11 (brs, 1H, H-1B), 5.09 (d, *J* = 3.9 Hz, H-1E'), 5.08 (d, *J* = 1.7 Hz, 1H, H-1A), 5.01–4.96 (m, 3H, H-2C, H-2C', H-1A'), 4.88 (brs, 1H, H-1C'), 4.87 (brs, 1H, H-1C), 4.79 (d, *J* = 8.5 Hz, 1H, H-1D'), 4.55 (d, *J* = 8.5 Hz, 1H, H-1D), 4.42 (tapp, *J* = 2.2 Hz, 1H, H-2A), 4.26 (tapp, *J* = 2.4 Hz, 1H, H-2A'), 4.12–3.99 (m, 6H, H-5C, H-5C', H-5E, H-2B, H-2B', OCH₂CH₂NH₂), 3.98–3.75 (m, 16H, H-5E', H-3C, H-3C', H-3A, H-6aD, H-6aD', OCH₂CH₂NH₂, H-3A', H-2D, H-2D', H-3E, H-3E', H-6aE, H-6aE', H-6bE, H-6bE'), 3.75–3.65 (m, 7H, H-6bD, H-6bD', H-5A', H-3B, H-3B', H-5A, H-2E), 3.62–3.49 (m, 9H, H-2E', H-4C, H-4C', H-5B, H-5B', H-4A', H-3D, H-4D, H-4D'), 3.50–3.36 (m, 7H, H-4E, H-4E', H-4B, H-4B', H-3D, H-5D, H-5D'), 3.33 (tapp, *J* = 9.8 Hz, 1H, H-4A), 3.26–3.13 (m, 2H, CH₂NH₂), 2.16 (s, 6H, HAC), 2.09 (s, 3H, HNCO), 2.04 (s, 3H, HNAC), 1.30–1.21 (m, 18H, H-6A, H-6A', H-6B, H-6B', H-6C, H-6C'). ¹³C NMR (100 MHz, D₂O) δ 177.0 (2C, CONCO), 175.7 (COAC), 175.6 (CONAC), 104.6 (C-1A', JC,H = 173.4 Hz), 104.2 (C-1D', JC,H = 164.1 Hz), 103.8 (C-1A, JC,H = 175.2 Hz), 103.6 (2C, C-1B, C-1B', JC,H = 174.8 Hz), 103.2 (C-1D, JC,H = 162.0 Hz), 101.2 (2C, C-1C, C-1C', JC,H = 173.5 Hz), 98.0 (C-1E', JC,H = 173.4 Hz), 97.0 (C-1E, JC,H = 170.7 Hz), 85.2 (C-3D'), 84.8 (C-3D), 80.9, 80.8 (2C, C-2B, C-2B'), 78.8, 78.6 (4C, C-3C, C-3C', C-5D, C-5D'), 77.9 (C-3A'), 76.8 (C-2A), 76.1 (C-3A), 75.8 (C-3E), 75.6 (C-3E'), 74.9 (2C, C-2C, C-2C'), 74.5 (2C, C-4B, C-4B'), 74.3 (4C, C-5E', C-2E', C-4C, C-4C'), 74.1, 73.9 (2C, C-5E, C-2E), 73.5 (C-4A), 72.9 (C-4A'), 72.6, 72.5 (2C, C-3B, C-3B'), 72.1, 72.0 (6C, C-5A, C-5A', C-5B, C-5B', C-4E, C-4E'), 71.6, 71.5 (2C, C-5C, C-5C'), 71.0, 70.8 (2C, C-4D, C-4D'), 69.3 (C-2A'), 68.3 (OCH₂CH₂CH₂NH₂), 63.3 (2C, C-6D, C-6D'), 63.0 (2C, C-6E, C-6E'), 58.0, 57.7 (2C, C-2D, C-2D') 42.1 (CH₂NH₂), 25.4 (CNAC), 24.9 (CNAC), 22.8 (CAC), 22.8 (2C, CAC), 19.5, 19.4, 19.2, 18.9 (6C, C-6A, C-6A', C-6B, C-6B', C-6C, C-6C'). HRMS (ESI⁺): *m/z* 1752.6962 (calcd for C₇₀H₁₁₇N₃O₄₇Na [M + H]⁺: *m/z* 1753.6969).

## Conflicts of interest

There are no conflicts to declare.
Acknowledgements

This work was supported by Institut Pasteur under grant agree-
ment GPH-FLEXBIVAC (postdoctoral fellowship to J.C.) and by
the European Union's seventh Framework Program for research,
technological development, and demonstration under grant
agreement no 261462-STOPENTERICS (postdoctoral fellowship
to Z.H.). The authors would like to acknowledge Ms Catherine
Guerreiro (Chemistry of Biomolecules) for her excellent advice
with regard to RP-HPLC. They also thank Mr Frédéric
Bonhomme (UMR3523 CNRS) for the HRMS spectra.

Notes and references

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