# Omic approaches to assess the ecotoxicological impact of synthetic and natural β-triketone herbicides on soil bacterial diversity and activity

Sana Romdhane, Marion Devers, Lise Barthelmebs, Cédric Bertrand, Marie-Virginie Salvia, Christophe Calvayrac, Franck E. Dayan, Fabrice Martin-Laurent

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## To cite this version:

Sana Romdhane, Marion Devers, Lise Barthelmebs, Cédric Bertrand, Marie-Virginie Salvia, et al. Omic approaches to assess the ecotoxicological impact of synthetic and natural β-triketone herbicides on soil bacterial diversity and activity. 16. International Symposium on Microbial Ecology - ISME16, International Society for Microbial Ecology (ISME). INT., Aug 2016, Montréal, Canada. hal-01604141

HAL Id: hal-01604141

https://hal.science/hal-01604141

Submitted on 3 Jun 2020

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Omic approaches to assess the ecotoxicological impact of synthetic and natural β-
triketone herbicides on soil bacterial diversity and activity

Sana Romdhane<fnref n="1" />,<fnref n="2" />,<fnref n="3" />, Marion Devers-Lamrani<fnref n="3" />, Lise Barthelmebs<fnref n="1" />, Cédric Bertrand<fnref n="2" />, Marie-Virginie Salvia <fnref n="2" />, Christophe Calvayrac<fnref n="1" />, Franck E. Dayan<fnref n="4" />, Fabrice Martin-Laurent<fnref n="3" />

**Abstract:**

Conventional agricultural practices rely on the generalized use of synthetic pesticides for managing weeds. However, there are increasing concerns regarding side effects caused by these substances on human health and ecosystems. In this context, the emergence of pesticides of natural origin (biopesticide) to control pests in the crop appears as an environmentally-friendly alternative. In order to verify these claims, leptospermone, a natural β-triketone herbicide, and sulcotrione, a synthetic one, were applied at 0× (control), 1× or 10× recommended field dose to soil microcosms. The fate of these two herbicides was monitored to assess the scenario of exposure of soil microorganisms. Ecotoxicological impact caused by both herbicides was explored by monitoring the evolution of (i) soil bacterial diversity by 16S rRNA gene amplicons pyrosequencing and (ii) the evolution of microbial activity by soil metabolomics. We showed that both leptospermone and sulcotrione were fully dissipated over the incubation period. However, during their dissipation metabolites of natural and synthetic β-triketone were detected, and CMBA the major metabolite of sulcotrione was remaining in soil microcosms by the end of the experiment. After 8 days of exposure, the bacterial diversity was significantly modified in response to leptospermone. Less significant changes were observed for sulcotrione. For both herbicides, the diversity of the soil bacterial community was not recovered 45 days after the treatment. In addition, soil metabolomics made possible the identification of potential biomarkers of β-triketone exposure. The use of omics applied to soil matrices allowed evaluating the impact of natural and synthetic pesticides on non target soil microorganisms.

**Keywords:** ecotoxicology, bacterial community, β-triketone, pyrosequencing, metabolomics.

<footnote n="1">¹Biocapteurs Analyse Environnement, EA 4218, Univ. Perpignan Via Domitia, F-66860, Perpignan, France.</footnote>

<footnote n="2">²CRIOBE, USR 3278 EPHE CNRS UPVD, Univ. Perpignan Via Domitia, F-66860, Perpignan, France.</footnote>

<footnote n="3">³INRA, UMR 1347 Agroécologie, EcolDur, BP 86510, 21065 Dijon, France.</footnote>

<footnote n="4">⁴Bioagricultural Sciences and Pest Management Department, Colorado State University, Fort Collins, Colorado, USA.</footnote>