# Are regulated cell death (RCD) pathways involved in neuronal death in TBEV-infected human neuronal/glial cells? A transcriptomic study

François Piumi, Fares Mazigh, Cochet Marielle, Jouneau Luc, Blanchet Odile, Coulpier Muriel

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François Piumi, Fares Mazigh, Cochet Marielle, Jouneau Luc, Blanchet Odile, et al. Are regulated cell death (RCD) pathways involved in neuronal death in TBEV-infected human neuronal/glial cells? A transcriptomic study. journées d'animation scientifique du département santé animale INRAE, Oct 2022, Anglet, France. hal-04803691

HAL Id: hal-04803691

https://hal.science/hal-04803691v1

Submitted on 25 Nov 2024

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Distributed under a Creative Commons Attribution 4.0 International License
# Necroptosis and pyroptosis are suggested to play a role in neuronal death after TBEV<fnref n="1" /> infection

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## Are regulated cell death (RCD) pathways involved in neuronal death in TBEV-infected human neuronal/glial cells? A transcriptomic study

### INTRODUCTION

* One of the major hallmark of TBEV infection is its tropism for neurons followed by neuronal death. Unfortunately, so far, the underlying molecular mechanisms of the neuronal death remain poorly understood<fnref n="2" />

### METHODS

* TBEV-infected human neuronal/glial cells differentiated from fetal neural progenitor cells<fnref n="2" />

* 3 time points: 24 hpi, 96 hpi and 7 dpi

* RNA-Seq (16 RNA samples)

* Diff. expression analysis (in-house developed RNA-Seq pipeline)

* IPA enrichment analysis / gene lists specific for each RCD pathway

### RESULTS

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### CONCLUSION

* Our results suggest that multiple mechanisms of regulated cell death concur to induce neuronal death in TBEV-infected neuronal/glial cells, namely: necroptosis, pyroptosis and apoptosis.

* They comfort previous results<fnref n="2" /> showing an involvement of pyroptosis and apoptosis and suggest an additional mechanisms (necroptosis) that was, so far, unknown.

* On the contrary, we have no evidence of involvement of ferroptosis and autophagy in TBEV-induced neuronal death.

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Differentially expressed genes in RCD pathways (differential expression threshold at log2FC = 2 indicated by the red dashed line)

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François Piumi<fnref n="1" />, Mazigh Fares<fnref n="2" />, Marielle Cochet-Bernoin<fnref n="1" />, Luc Jouneau³, Odile Blanchet⁴, Muriel Coulpier<fnref n="1" />

1: UMR1161 Virologie, Anses, INRAE, ENVA, Université Paris-Est, Maisons-Alfort. 2: MRC-University of Glasgow Centre for Virus Research, Glasgow, Scotland, United Kingdom. 3: VIM, Université Paris-Saclay, INRAE, UVSQ, Domaine de Vilvert, Jouy-en-Josas. 4: CHU Angers, CRB, Angers.

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<footnote n="1">¹Tick-Borne Encephalitis Virus</footnote>

<footnote n="2">²Fares et al. Viruses 2021, 13, 2255. https://doi.org/10.3390/v13112255</footnote>