# Bulletin de veille du réseau d'écotoxicologie terrestre et aquatique N°53

Colette Bertrand, Christian Mougin, Annette Bérard, Soizic Morin, Olivier

Crouzet, Pascale Karmasyn-Veyrines

► To cite this version:

Colette Bertrand, Christian Mougin, Annette Bérard, Soizic Morin, Olivier Crouzet, et al.. Bulletin de veille du réseau d'écotoxicologie terrestre et aquatique N°53. 2021, 10.15454/bwf7-8m60. hal-03284378

HAL Id: hal-03284378

https://hal.inrae.fr/hal-03284378v1

Submitted on 22 Nov 2021

HAL is a multi-disciplinary open access
archive for the deposit and dissemination of sci-
entific research documents, whether they are pub-
lished or not. The documents may come from
teaching and research institutions in France or
abroad, or from public or private research centers.

L'archive ouverte pluridisciplinaire HAL, est
destinée au dépôt et à la diffusion de documents
scientifiques de niveau recherche, publiés ou non,
émanant des établissements d'enseignement et de
recherche français ou étrangers, des laboratoires
publics ou privés.

<figure><img src="image_1.png" /></figure>

Distributed under a Creative Commons CC BY-SA 4.0 - Attribution - ShareAlike - International
License
# Bulletin de veille du réseau d'écotoxicologie terrestre et aquatique

<figure><img src="image_2.png" /></figure>

N°53, Octobre 2021

Réalisé par l'équipe de veille sur la période du 1er septembre au 31 octobre 2021.

Colette Bertrand, Christian Mougin (UMR 1402 EcoSys), Annette Berard (UMR 1114 EMMAH), Soizic Morin (UR 1454 EABX), Olivier Crouzet (UPFS - OFB) et Pascale Karmasyn-Veyrines (DipSO)

## Edito

Voici notre 53ème bulletin de veille, qui nous espérons toujours informatif !

Nous vous proposons dans ce bulletin une tribune concernant la méthode PICT (Pollution-Induced Community Tolerance), un outil complémentaire pour l'évaluation du risque et le biomonitoring des pesticides. La tribune est téléchargeable sous forme de fiche thématique sur notre site ECOTOX : https://www6.inrae.fr/ecotox/Productions/Fiches-thematiques/Fiche-thematique-N-35-octobre-2021

Elle a été rédigée dans le cadre de l'expertise collective « Phytopharmaceutiques Biodiversité Services Ecosystémiques » : https://www6.inrae.fr/ecotox/Productions/Fiches-thematiques/Fiche-thematique-N-29-octobre-2020

Nous vous rappelons notre PCI pour la soumission de vos preprints : https://ecotoxenvchemŁpeercommunityin.org/

N'oubliez pas de nous transmettre les informations que vous souhaitez diffuser, notamment vos publications que nous pourrions avoir oubliées.

L'équipe vous souhaite une bonne lecture de ce bulletin !

Contact : veille-ecotox@inrae.fr
<table><tr><td>on the pathways of secondary metabolites</td><td>34</td></tr><tr><td>Single and Combined Effects of Cypermethrin and UVR Pre-Exposure in the Microalgae Phaeodactylum Tricornutum</td><td>35</td></tr><tr><td>Impact of wastewater on the microbial diversity of periphyton and its tolerance to micropollutants in an engineered flow-through channel system</td><td>35</td></tr><tr><td>Toxicities of three metal oxide nanoparticles to a marine microalga: Impacts on the motility and potential affecting mechanisms</td><td>35</td></tr><tr><td>Microbial diversity alteration reveals biomarkers of contamination in soil-river-lake continuum</td><td>35</td></tr><tr><td>Temperature, phytoplankton density and bacteria diversity drive the biotransformation of micropollutants in a lake ecosystem</td><td>36</td></tr><tr><td>A review on catalytic-enzyme degradation of toxic environmental pollutants: Microbial enzymes</td><td>36</td></tr><tr><td>The effects of copper ions and copper nanomaterials on the output of amino acids from marine microalgae</td><td>36</td></tr><tr><td>Bacterial survival strategies and responses under heavy metal stress: a comprehensive overview</td><td>37</td></tr><tr><td>BactoTraits - A functional trait database to evaluate how natural and man-induced changes influence the assembly of bacterial communities</td><td>37</td></tr><tr><td>Odiel River (SW Spain), a Singular Scenario Affected by Acid Mine Drainage (AMD): Graphical and Statistical Models to Assess Diatoms and Water Hydrogeochemistry Interactions</td><td>37</td></tr><tr><td>Intraspecific variation in metal tolerance modulate competition between two marine diatoms</td><td>38</td></tr><tr><td>Marine biofilms on different fouling control coating types reveal differences in microbial community composition and abundance</td><td>38</td></tr><tr><td>Benthic microbial diversity trends in response to heavy metals in an oxygen-deficient eutrophic bay of the Humboldt current system offshore the Atacama Desert</td><td>38</td></tr><tr><td>Effect of heavy metals on protein content of marine unicellular green alga Dunaliella tertiolecta</td><td>38</td></tr><tr><td>Environmentally relevant fungicide levels modify fungal community composition and interactions but not functioning</td><td>39</td></tr><tr><td>Specific toxicity of azithromycin to the freshwater microalga Raphidocelis subcapitata</td><td>39</td></tr></table>

<table><tr><td>ERA / PUBLICATIONS SCIENTIFIQUES / ECOTOXICOLOGIE SPATIALE</td><td>39</td></tr><tr><td>High spatial resolution measurements of passive-sampler derived air concentrations of persistent organic pollutants in the Campania region, Italy: Implications for source identification and risk analysis</td><td>39</td></tr><tr><td>Application of land use regression modelling to describe atmospheric levels of semivolatile organic compounds on a national scale</td><td>40</td></tr><tr><td>Occurrence and risk assessment of pesticides in a Mediterranean Basin with strong agricultural pressure (Guadiana Basin: Southern of Portugal)</td><td>40</td></tr><tr><td>A spatially explicit model for estimating risks of pesticide exposure to bird populations</td><td>40</td></tr><tr><td>Supporting non-target arthropods in agroecosystems: Modelling effects of insecticides and landscape structure on carabids in agricultural landscapes</td><td>40</td></tr></table>
<table><tr><td>soils: An in situ field experiment</td><td>46</td></tr><tr><td>Effects of Heavy Metals/Metalloids and Soil Properties on Microbial Communities in Farmland in the Vicinity of a Metals Smelter</td><td>46</td></tr><tr><td>Response of Soil Microbial Communities to Different Doses of Glyphosate and Sulfosulfuron in a Calcareous Soil</td><td>46</td></tr><tr><td>Heavy metal pollution increases soil microbial carbon limitation: Evidence from ecological enzyme stoichiometry</td><td>47</td></tr><tr><td>The Variations of Bacterial Community Structures in Tailing Soils Suffering from Heavy Metal Contaminations</td><td>47</td></tr><tr><td>Discovery and characterization of UipA, a uranium- and iron-binding PepSY protein involved in uranium tolerance by soil bacteria</td><td>47</td></tr><tr><td>Response of soil enzyme activity and bacterial community to copper hydroxide nanofertilizer and its ionic analogue under single versus repeated applications</td><td>47</td></tr><tr><td>Comprehensive characterization of stress tolerant bacteria with plant growth-promoting potential isolated from glyphosate-treated environment</td><td>48</td></tr><tr><td>Evaluating the metabolic functional profiles of the microbial community and alfalfa (Medicago sativa) traits affected by the presence of carbon nanotubes and antimony in drained and waterlogged sediments</td><td>48</td></tr><tr><td>Vertical distribution and effect of historical residual organochlorine pesticides on microbial community structure in sediment cores from an abandoned oxidation pond after dredging for 15 years</td><td>48</td></tr><tr><td>When nanoparticle and microbes meet: The effect of multi-walled carbon nanotubes on microbial community and nutrient cycling in hyperaccumulator system</td><td>48</td></tr><tr><td>Ecological network analysis reveals distinctive microbial modules associated with heavy metal contamination of abandoned mine soils in Korea</td><td>49</td></tr><tr><td>Ant nests as a microbial hot spots in a long-term heavy metal-contaminated soils</td><td>49</td></tr><tr><td>Microbial diversity alteration reveals biomarkers of contamination in soil-river-lake continuum</td><td>49</td></tr><tr><td>Changes in the Composition of the Soil Bacterial Community in Heavy Metal-Contaminated Farmland</td><td>49</td></tr><tr><td>Cadmium retention and microbial response in volcanic soils along gradients of soil age and climate on the Galapagos Islands</td><td>50</td></tr><tr><td>Microbial Community's Dynamic Response to Fomesafen Usage in Chernozems of Northeast China</td><td>50</td></tr><tr><td>Effect of an Equal Dose of Polymetallic Pollution on the Microbiological Characteristics of Two Soils with Different Organic Carbon Contents</td><td>50</td></tr><tr><td>Dinotefuran alters Collembola-fungi-bacteria interactions that control mineralization of maize and soil organic carbon</td><td>50</td></tr><tr><td>Microbiological Nitrogen Transformations in Soil Treated with Pesticides and Their Impact on Soil Greenhouse Gas Emissions</td><td>51</td></tr><tr><td>Effects of heavy metals and organic matter fractions on the fungal communities in mangrove sediments from Techeng Isle, South China</td><td>51</td></tr></table>
ERA / PUBLICATIONS SCIENTIFIQUES / MICROBIOLOGIE ET CONTAMINANTS / ANTIBIOTIQUES ET ANTIBIORESISTANCES 51

Incubation trial indicated the earthworm intestinal bacteria as promising biodigestor for mitigating tetracycline resistance risk in anthropogenic disturbed forest soil 51

Sulfamethoxazole biodegradation and impacts on soil microbial communities in a Bolivian arid high altitude catchment 51

Fertilization with poultry litter increases the abundance of antibiotic-resistant bacteria in tropical soil: a microcosm study 52

Antibiotic resistance, antimicrobial residues, and bacterial community diversity in pasture-raised poultry, swine, and beef cattle manures 52

Impact of chicken litter pre-application treatment on the abundance, field persistence, and transfer of antibiotic resistant bacteria and antibiotic resistance genes to vegetables 52

Assessing Antibiotics Biodegradation and Effects at Sub-inhibitory Concentrations by Quantitative Microbial Community Deconvolution 53

Manure microbial communities and resistance profiles reconfigure after transition to manure pits and differ from those in fertilized field soil 53

Insights into the role of the fungal community in variations of the antibiotic resistome in the soil collembolan gut microbiome 53

From the soil to the clinic: the impact of microbial secondary metabolites on antibiotic tolerance and resistance 53

Distribution and Influence on the Microbial Ecological Relationship of Antibiotic Resistance Genes in Soil at a Watershed Scale 54

Persistence and spread of tetracycline resistance genes and microbial community variations in the soil of animal corrals in a semi-arid planted forest 54

Post-digestate composting shifts microbial composition and degrades antimicrobial resistance genes 54

Insights into the mechanism of the interference of sulfadiazine on soil microbial community and function 54

The Effect of Clarithromycin Toxicity on the Growth of Bacterial Communities in Agricultural Soils 55

Enzymatic activity and microbial diversity of sod-podzolic soil microbiota using 16s rrna amplicon sequencing following antibiotic exposure 55

Antibiotic Resistance Gene-Carrying Plasmid Spreads into the Plant Endophytic Bacteria using Soil Bacteria as Carriers 55

ERA / PUBLICATIONS SCIENTIFIQUES / MICROBIOLOGIE ET CONTAMINANTS / BIOCONTROLE 55

Using crop diversity to lower pesticide use: Socio-ecological approaches 55

Impacts of decaying aromatic plants on the soil microbial community and on tomato seedling growth and metabolism: suppression or stimulation? 56

Benefits of corn-cob biochar to the microbial and enzymatic activity of soybean plants grown in soils contaminated with heavy metals 56
<table><tr><td>Biopesticide evaluation from lab to greenhouse scale of essential oils used against macrosiphum euphorbiae</td><td>56</td></tr><tr><td>Apiaceae essential oils and their constituents as insecticides against mosquitoes-A review</td><td>56</td></tr><tr><td>Impact of biocontrol microbes on soil microbial diversity in ginger (Zingiber officinale Roscoe)</td><td>57</td></tr></table>

ERA / PUBLICATIONS SCIENTIFIQUES / MICROBIOLOGIE ET CONTAMINANTS / BIOREMEDIATION

<table><tr><td>System biology analysis of endosulfan biodegradation in bacteria and its effect in other living systems: modeling and simulation studies</td><td>57</td></tr><tr><td>Photo-biodegradation of imidacloprid under blue light-emitting diodes with bacteria and co-metabolic regulation</td><td>57</td></tr><tr><td>A field study reveals links between hyperaccumulating Sedum plants-associated bacterial communities and Cd/Zn uptake and translocation</td><td>57</td></tr><tr><td>Functional analysis of chlorpyrifos biodegradation in agricultural soils augmented with a three-strain bacterial consortium</td><td>58</td></tr><tr><td>Endophytic bacterium CIMAP-A7 mediated amelioration of atrazine induced phyto-toxicity in Andrographis paniculata</td><td>58</td></tr><tr><td>Assessing the arsenic-saturated biochar recycling potential of vermitechnology: Insights on nutrient recovery, metal benignity, and microbial activity</td><td>58</td></tr><tr><td>Disentangling the role of ectomycorrhizal fungi in plant nutrient acquisition along a Zn gradient using X-ray imaging</td><td>58</td></tr><tr><td>An efficient manganese-oxidizing fungus Cladosporium halotolerans strain XM01: Mn(II) oxidization and Cd adsorption behavior</td><td>58</td></tr><tr><td>A biogenic microbial biosurfactin that degrades difenoconazole fungicide with potential antimicrobial and oil displacement properties</td><td>59</td></tr><tr><td>Biochar and earthworms synergistically improve soil structure, microbial abundance, activities and pyraclostrobin degradation</td><td>59</td></tr><tr><td>Theoretical design and process control of neonicotinoids insecticides suitable for synergistic degradation with the rubisco enzyme from rhizobia and carbon-fixing bacteria in soil</td><td>59</td></tr><tr><td>Enhanced Bioremediation of Heavy Metals from Phosphate Processing Wastewater Using the Indigenous Bacterium Serratia rubidaea NCTC12971</td><td>59</td></tr><tr><td>Bacterial consortium biotransformation of pentachlorophenol contaminated wastewater</td><td>60</td></tr><tr><td>Passivation of multiple heavy metals in lead-zinc tailings facilitated by straw biochar-loaded N-doped carbon aerogel nanoparticles: Mechanisms and microbial community evolution</td><td>60</td></tr><tr><td>Microbial Community Diversity Dynamics in Acid Mine Drainage and Acid Mine Drainage-Polluted Soils: Implication on Mining Water Irrigation Agricultural Sustainability</td><td>60</td></tr><tr><td>Microbial investigations of new hydrogel-biochar composites as soil amendments for simultaneous nitrogen-use improvement and heavy metal immobilization</td><td>60</td></tr><tr><td>Interactions among heavy metal bioaccessibility, soil properties and microbial community in phyto-remediated soils nearby an abandoned realgar mine</td><td>61</td></tr></table>
<table><tr><td><i>lanceolata</i></td><td>67</td></tr><tr><td>Biodegradation of low-density polyethylene (LDPE) sheet by microalga, <i>Uronema africanum</i> Borge</td><td>67</td></tr><tr><td>A review on the microbial degradation of chlorpyrifos and its metabolite TCP</td><td>67</td></tr><tr><td>Arbuscular mycorrhizal fungi reduce cadmium accumulation in plants: evidence and uncertainty</td><td>67</td></tr><tr><td>Culture-dependent study of arsenic-reducing bacteria in deep aquatic sediments of Bengal Delta</td><td>68</td></tr><tr><td>Comparative Investigation of 15 Xenobiotic-Metabolizing N-Acetyltransferase (NAT) Homologs from Bacteria</td><td>68</td></tr><tr><td>Inhibition of cadmium releasing from sulfide tailings into the environment by carbonate-mineralized bacteria</td><td>68</td></tr><tr><td>Glyphosate-degrading behavior of five bacterial strains isolated from stream biofilms</td><td>68</td></tr><tr><td>Lowered Cd toxicity, uptake and expression of metal transporter genes in maize plant by ACC deaminase-producing bacteria <i>Achromobacter sp</i></td><td>68</td></tr><tr><td>Succession of enzymes and microbial biomarkers in the process of vermicomposting: An insight towards valorization of toxic paper mill wastes using <i>Perionyx excavates</i> (Oligochaeta; Perrier, 1872)</td><td>69</td></tr><tr><td>The effect of combined pollution by PAHs and heavy metals on the topsoil microbial communities of Spolic Technosols of the lake Atamanskoe, Southern Russia</td><td>69</td></tr><tr><td>Microbial reduction of Cr(VI) in the presence of Ni, Cu and Zn by bacterial consortium enriched from an electroplating contaminated site</td><td>69</td></tr><tr><td>Biochar aging alters the bioavailability of cadmium and microbial activity in acid contaminated soils</td><td>69</td></tr><tr><td>Microbial response and adaption to thallium contamination in soil profile</td><td>70</td></tr><tr><td>Plant-microbial remediation of chlorpyrifos contaminated soil</td><td>70</td></tr><tr><td>Evaluation of the PGPR Capacity of Four Bacterial Strains and Their Mixtures, Tested on Lupinus albus var. Dorado Seedlings, for the Bioremediation of Mercury-Polluted Soils</td><td>70</td></tr><tr><td>Bioaugmentation and bioaugmentation-assisted phytoremediation of heavy metal contaminated soil by a synergistic effect of cyanobacteria inoculation, biochar, and purslane (<i>Portulaca oleracea</i> L.)</td><td>70</td></tr><tr><td>Ectomycorrhizal fungi enhance the tolerance of phytotoxicity and cadmium accumulation in oak (<i>Quercus acutissima</i> Carruth.) seedlings: modulation of growth properties and the antioxidant defense responses</td><td>71</td></tr><tr><td>Deciphering the endophytic and rhizospheric microbial communities of a metallophyte commelina communis in different cu-polluted soils</td><td>71</td></tr><tr><td>Co-metabolic and biochar-promoted biodegradation of mixed PAHs by highly efficient microbial consortium QY1</td><td>71</td></tr><tr><td>Isolation and development of a microbial consortium for the treatment of automobile service station wastewater</td><td>71</td></tr><tr><td>Remediation of Petroleum-Contaminated Soils with Microbial and Microbial Combined Methods: Advances, Mechanisms, and Challenges</td><td>72</td></tr><tr><td>Isolation, characterization, and evaluation of a high-siderophore-yielding bacterium from heavy metal-contaminated soil</td><td>72</td></tr><tr><td>Experimental study on treatment of heavy metal-contaminated soil by manganese-oxidizing bacteria</td><td>72</td></tr><tr><td>Lead pollution and bacterial bioremediation: a review</td><td>72</td></tr><tr><td>Copper (II) Ion Action on Soil Bacteria</td><td>72</td></tr></table>
<table><tr><td>The Terrestrial Plastisphere: Diversity and Polymer-Colonizing Potential of Plastic-Associated Microbial Communities in Soil</td><td>78</td></tr><tr><td>Assessing the risks of potential bacterial pathogens attaching to different microplastics during the summer-autumn period in a mariculture cage</td><td>79</td></tr><tr><td>Characterization and Molecular Identification of Poly Urethane Degrading Bacteria</td><td>79</td></tr><tr><td>Analysis of microbial populations in plastic-soil systems after exposure to high poly(butylene succinate-co-adipate) load using high-resolution molecular technique</td><td>79</td></tr><tr><td>Polystyrene nanoplastics change the functional traits of biofilm communities in freshwater environment revealed by GeoChip 5.0</td><td>79</td></tr><tr><td>Environmental behaviors of microplastics in aquatic systems: A systematic review on degradation, adsorption, toxicity and biofilm under aging conditions</td><td>80</td></tr><tr><td>Presence of polyethylene terephthalate (PET) fibers in hyporheic zone alters colonization patterns and seasonal dynamics of biofilm metabolic functioning</td><td>80</td></tr><tr><td>Aquatic Biofilms-Sink or Source of Microplastics? A Critical Reflection on Current Knowledge-Web of Science Core Collection</td><td>80</td></tr><tr><td>Biogeography rather than substrate type determines bacterial colonization dynamics of marine plastics</td><td>81</td></tr><tr><td>The structure and assembly mechanisms of plastisphere microbial community in natural marine environment</td><td>81</td></tr><tr><td>The plastic Trojan horse: Biofilms increase microplastic uptake in marine filter feeders impacting microbial transfer and organism health</td><td>81</td></tr><tr><td>Microbial colonizers of microplastics in an Arctic freshwater lake</td><td>81</td></tr><tr><td>Parental exposure to sulfamethazine and nanoplastics alters the gut microbial communities in the offspring of marine madaka (Oryzias melastigma)</td><td>82</td></tr><tr><td>Soil pH has a stronger effect than arsenic content on shaping plastisphere bacterial communities in soil</td><td>82</td></tr><tr><td>Deciphering the diversity and functions of plastisphere bacterial communities in plastic-mulching croplands of subtropical China</td><td>82</td></tr><tr><td>Assessing the presence of microplastic particles in Tunisian agriculture soils and their potential toxicity effects using Eisenia andrei as bioindicator</td><td>82</td></tr><tr><td>Cross-Hemisphere Study Reveals Geographically Ubiquitous, Plastic-Specific Bacteria Emerging from the Rare and Unexplored Biosphere</td><td>83</td></tr><tr><td>Microbial biofilm composition and polymer degradation of compostable and non-compostable plastics immersed in the marine environment</td><td>83</td></tr><tr><td>Biodegradation of low-density polyethylene (LDPE) sheet by microalga, Uronema africanum Borge</td><td>83</td></tr><tr><td>The effect of salinity on the interaction between microplastic polyethylene terephthalate (PET) and microalgae Spirulina sp.</td><td>83</td></tr><tr><td>Polystyrene microplastics induce microbial dysbiosis and dysfunction in surrounding seawater</td><td>84</td></tr><tr><td>Deep-sea plastisphere: Long-term colonization by plastic-associated bacterial and archaeal communities in the Southwest Atlantic Ocean</td><td>84</td></tr></table>
<table><tbody><tr><td>LMR de 6-benzyladénine et d'aminopyralide</td><td>90</td></tr><tr><td>LMR de flupyradifurone et d'acide difluoroacétique</td><td>90</td></tr><tr><td>Limites maximales applicables aux résidus de pesticides présents dans ou sur certains produits</td><td>90</td></tr><tr><td>LMR aclonifène, d'acrinathrine, de Bacillus pumilus QST 2808, d'éthirimol, de penthiopyrade, de piclorame et de Pseudomonas</td><td>90</td></tr><tr><td>Rectification du numéro CAS de la substance de base «chlorhydrate de chitosane</td><td>91</td></tr><tr><td>Approbation renouvelée de la substance active à faible risque «carbonate de calcium»</td><td>91</td></tr><tr><td>Approbation prolongée des substances actives amidosulfuron, bifénox, chlorméquat, chlorotoluron, clofentézine, clomazone, cyperméthrine, daminozide, deltaméthrine, dicamba, difénoconazole, diflufénican, diméthachlore, étofenprox, fenoxaprop-P, fenpropidine, fludioxonyl, flufénacet, fosthiazate, huile de paraffine, huiles de paraffine, hydroxy-8-quinoléine, indoxacarbe, lénacile, MCPA, MCPB, nicosulfuron, penconazole, phénylphénol-2</td><td>91</td></tr><tr><td>Substances actives «acrinathrine» et «prochloraz» : période d'approbation modifiée</td><td>91</td></tr><tr><td>Approbation renouvelée de la substance active «hydrogénocarbonate de potassium» en tant que substance à faible risque</td><td>92</td></tr><tr><td>Non-approbation du sulfure de diméthyle en tant que substance de base</td><td>92</td></tr><tr><td>Approbation de la substance active à faible risque «Bacillus amyloliquefaciens souche AH2»</td><td>92</td></tr><tr><td>REGLEMENTATION / DEBAT</td><td>92</td></tr><tr><td>Seuils des polluants organiques persistants : appel à consultation de la commission</td><td>92</td></tr><tr><td>New EU farm to fork strategy to make our food healthier and more sustainable</td><td>93</td></tr><tr><td>Rapport sur une stratégie «De la ferme à la table» pour un système alimentaire équitable, sain et respectueux de l'environnement - A9-0271/2021</td><td>93</td></tr><tr><td>AVIS / EXPERTISES / NORMES</td><td>93</td></tr><tr><td>Non-conformités dans les eaux destinées à la consommation humaine dues aux métabolites du métolachlore</td><td>93</td></tr><tr><td>New dual screening method for emerging pollutant chemicals in urban wastewater, Lyon, France</td><td>93</td></tr><tr><td>Identifier les voies de transfert de l'AMPA vers un captage AEP pour mieux gérer la contamination</td><td>93</td></tr><tr><td>Les chiffres clés de la Haute Valeur Environnementale (HVE)</td><td>94</td></tr><tr><td>PUBLICATIONS DU RESEAU ECOTOX</td><td>94</td></tr><tr><td>Molecular Epidemiology of Azole-Resistant Aspergillus fumigatus in France Shows Patient and Healthcare Links to Environmentally Occurring Genotypes</td><td>94</td></tr><tr><td>Silicon (Si) Alleviates Iron Deficiency Effects in Sea Barley (Hordeum marinum) by Enhancing Iron Accumulation and Photosystem Activities</td><td>94</td></tr><tr><td>Bt cotton area contraction drives regional pest resurgence, crop loss, and pesticide use</td><td>95</td></tr><tr><td>Assessment of exposure to ionizing radiation in Chernobyl tree frogs (Hyla orientalis)</td><td>95</td></tr></tbody></table>
<table><tr><td>Emerging concepts and opportunities for endocrine disruptor screening of the non-EATS modalities</td><td>95</td></tr><tr><td>A cross-species comparative approach to assessing multi- and transgenerational effects of endocrine disrupting chemicals</td><td>95</td></tr><tr><td>Development of an exposure protocol for toxicity test (FEET) for a marine species: the European sea bass (Dicentrarchus labrax)</td><td>96</td></tr><tr><td>Miniaturization of an extraction protocol for the monitoring of pesticides and polar transformation products in biotic matrices</td><td>96</td></tr><tr><td>Deciphering Differential Life Stage Radioinduced Reproductive Decline in Caenorhabditis elegans through Lipid Analysis</td><td>96</td></tr><tr><td>The combination of chemical, structural, and functional indicators to evaluate the anthropogenic impacts on agricultural stream ecosystems</td><td>96</td></tr><tr><td>Short- and long-term impacts of anaerobic digestate spreading on earthworms in cropped soils</td><td>97</td></tr><tr><td>Experimental evolution supports the potential of neonicotinoid-pyrethroid combination for managing insecticide resistance in malaria vectors</td><td>97</td></tr><tr><td>Toxic effects of a mixture of five pharmaceutical drugs assessed using Fontinalis antipyretica Hedw.</td><td>97</td></tr><tr><td>Direct photodegradation of 36 organic micropollutants under simulated solar radiation: Comparison with free-water surface constructed wetland and influence of chemical structure</td><td>98</td></tr><tr><td>An integrated metabolomics and proteogenomics approach reveals molecular alterations following carbamazepine exposure in the male mussel Mytilus galloprovincialis</td><td>98</td></tr><tr><td>A review of the effects of contamination and temperature in Solea solea larvae. Modeling perspectives in the context of climate change</td><td>98</td></tr><tr><td>Comparative developmental toxicity of conventional oils and diluted bitumen on early life stages of the rainbow trout (Oncorhynchus mykiss)</td><td>99</td></tr><tr><td>Biochar Effects on Soil-Plant Properties after Six Month Adaptation in Soil</td><td>99</td></tr><tr><td>The toxicity of cadmium-copper mixtures on daphnids and microalgae analyzed using the Biotic Ligand Model</td><td>99</td></tr><tr><td>Can Lumbricus terrestris be released in forest soils degraded by compaction? Preliminary results from laboratory and field experiments</td><td>99</td></tr><tr><td>Editorial: Invertebrate UDP-Glycosyltransferases: Nomenclature, Diversity and Functions</td><td>100</td></tr><tr><td>Increasing the power of interpretation for soil metaproteomics data</td><td>100</td></tr><tr><td>The science behind the ban: the outstanding impact of ecotoxicological research on the regulation of neonicotinoids</td><td>100</td></tr><tr><td>Water table fluctuations affect dichloromethane biodegradation in lab-scale aquifers contaminated with organohalides</td><td>100</td></tr><tr><td>Combined influences of transgenerational effects, temperature and insecticide on the moth Spodoptera littoralis</td><td>101</td></tr><tr><td>Glyphosate-degrading behavior of five bacterial strains isolated from stream biofilms</td><td>101</td></tr><tr><td>Effects of metals and persistent organic pollutants on the fitness and health of juveniles of the endangered european</td><td></td></tr></table>
<table><tr><td>sturgeon Acipenser sturio Exposed to W1ater and sediments of the Garonne and Dordogne rivers</td><td>101</td></tr><tr><td>Conflicts of interest and improvement through peer review: the case of IPBES report on pollinators</td><td>102</td></tr><tr><td>Characterization and quantification of chlordecone elimination in ewes</td><td>102</td></tr><tr><td>Quantification of multi-scale links of anthropogenic pressures with PAH and PCB bioavailable contamination in French freshwaters</td><td>102</td></tr><tr><td>Subchronic Exposure to Environmental Concentrations of Chlorpyrifos Affects Swimming Activity of Rainbow Trout Larvae</td><td>102</td></tr><tr><td>Discovery and characterization of UipA, a uranium- and iron-binding PepSY protein involved in uranium tolerance by soil bacteria</td><td>103</td></tr><tr><td>Fluorescence excitation/emission matrices as a tool to monitor the removal of organic micropollutants from wastewater effluents by adsorption onto activated carbon</td><td>103</td></tr><tr><td>Do DOM optical parameters improve the prediction of copper availability in vineyard soils?</td><td>103</td></tr><tr><td>Abundance of insects and aerial insectivorous birds in relation to pesticide and fertilizer use</td><td>104</td></tr><tr><td>Polyethylene microplastic toxicity to the terrestrial snail Cantareus aspersus: size matters</td><td>104</td></tr><tr><td>Transgenerational Effects of a Neonicotinoid and a Novel Sulfoximine Insecticide on the Harlequin Ladybird</td><td>104</td></tr><tr><td>Environmentally Relevant Mixture of Pesticides Affect Mobility and DNA Integrity of Early Life Stages of Rainbow Trout (Oncorhynchus mykiss)</td><td>104</td></tr><tr><td>Photodegradation of methotrexate in aqueous solution: degradation kinetics and identification of transformation products</td><td>105</td></tr><tr><td>Seasonal diet-based resistance to anticoagulant rodenticides in the fossorial water vole (Arvicola amphibius)</td><td>105</td></tr><tr><td>Phytomanagement of Metal(loid)-Contaminated Soils: Options, Efficiency and Value</td><td>105</td></tr><tr><td>Assessment of heavy metal pollution transfer and human exposure risks from the consumption of chicken grown in mining-surrounding areas</td><td>105</td></tr><tr><td>Comment on 'Ecotoxicity of copper input and accumulation for soil biodiversity in vineyards' by Karimi et al. (2021)</td><td>106</td></tr><tr><td>Lethal and sublethal effects of diluted bitumen and conventional oil on fathead minnow (Pimephales promelas) larvae exposed during their early development</td><td>106</td></tr><tr><td>Early life neonicotinoid exposure results in proximal benefits and ultimate carryover effects</td><td>106</td></tr><tr><td>One and multi-compartments toxico-kinetic modeling to understand metals' organotropism and fate in Gammarus fossarum</td><td>106</td></tr><tr><td>Development in the European flounder (Platichthys flesus) of a q-PCR assay for the measurement of telomere length, a potential biomarker of pollutant effects for biomonitoring studies</td><td>107</td></tr><tr><td>In vitro study to evaluate the antimicrobial activity of various multifunctional cosmetic ingredients and chlorphenesin on bacterial species at risk in the cosmetic industry</td><td>107</td></tr><tr><td>Profiling metal contamination from ultramafic sediments to biota along the Albanian shoreline of Lake Ohrid (Albania/Macedonia)</td><td>107</td></tr><tr><td>Subcellular Distribution of Dietary Methyl-Mercury in Gammarus fossarum and Its Impact on the Amphipod</td><td>107</td></tr></table>
<table><tr><td>Proteome</td><td>108</td></tr></table>

<table><tr><td>OUVRAGES / RAPPORTS / ACTES DE CONGRES</td><td>108</td></tr></table>

<table><tr><td>Polluants chimiques dans les milieux aquatiques : l'Ineris et l'OFB publient les résultats de la surveillance de nouvelles substances à l'échelle nationale</td><td>108</td></tr></table>

<table><tr><td>Organic Pollutants: Toxicity and Solutions</td><td>108</td></tr></table>

<table><tr><td>Sustainable Agriculture</td><td>109</td></tr></table>

<table><tr><td>Étude d'exposition aux pesticides chez les riverains de zones viticoles et non viticoles. Bilan de l'étude pilote</td><td>109</td></tr></table>

<table><tr><td>Pharmaceuticals and endocrine disruptors - transfer from water to land ecosystems</td><td>109</td></tr></table>

<table><tr><td>Une BD pour informer sur les perturbateurs endocriniens</td><td>109</td></tr></table>

<table><tr><td>Toxicology of Organophosphate Poisoning</td><td>110</td></tr></table>

<table><tr><td>Microplastic in the Environment: Pattern and Process</td><td>110</td></tr></table>

<table><tr><td>Pesticides, Organic Contaminants, and Pathogens in Air: Chemodynamics, Health Effects, Sampling, and Analysis</td><td>110</td></tr></table>

<table><tr><td>Projet #MacroPLAST</td><td>111</td></tr></table>

<table><tr><td>Regulatory Governance in the Pesticide Sector in Mexico</td><td>111</td></tr></table>

<table><tr><td>New Frontiers in Environmental Toxicology</td><td>111</td></tr></table>

<table><tr><td>Environmental Contamination in Antarctica - 1st Edition: a challenge to analytical chemistry</td><td>111</td></tr></table>

<table><tr><td>A chemical category-based approach for selecting and screening pfas for toxicity and toxicokinetic testing (presentation)</td><td>112</td></tr></table>

<table><tr><td>La santé-environnement : recherche, expertise et décision publiques</td><td>112</td></tr></table>

<table><tr><td>Drivers of and pressures arising from selected key water management challenges: a European overview</td><td>112</td></tr></table>

<table><tr><td>REVUE DE PRESSE / ALTERNATIVES / BIOPESTICIDES</td><td>112</td></tr></table>

<table><tr><td>Proliant : première solution de biocontrôle pour un démarrage sécurisé du maïs</td><td>112</td></tr></table>

<table><tr><td>Essais encourageants pour un futur produit de biocontrôle en céréales</td><td>113</td></tr></table>

<table><tr><td>REVUE DE PRESSE / ASSOCIATIONS</td><td>113</td></tr></table>

<table><tr><td>NGO group opposes the renewal of two endocrine disrupting pesticides at the PAFF meeting on 21-22 October !</td><td>113</td></tr></table>

<table><tr><td>Women in agricultural work at increased risk for skin and blood cancers from pesticide exposure</td><td>113</td></tr></table>

<table><tr><td>EPA to Create Advisory Councils to Restore Scientific Integrity in Pesticide/Chemicals Division</td><td>113</td></tr></table>

<table><tr><td>Common insecticide malathion linked to chronic kidney disease</td><td>113</td></tr></table>

<table><tr><td>Générations Futures fantasme sur des études « manquantes »</td><td>114</td></tr></table>

<table><tr><td>Climate pollution from plastics to outpace coal emissions in US by 2030, report finds</td><td>114</td></tr></table>

<table><tr><td>Exposed: How big farm lobbies undermine EU's green agriculture plan</td><td>114</td></tr></table>
<table><tr><td>Pacte Vert et Farm to fork La stratégie de l'UE, une trajectoire « réaliste » pour l'agriculture (Iddri)</td><td>114</td></tr><tr><td>L'Europe défend sa stratégie De la ferme à la fourchette</td><td>114</td></tr><tr><td>In utero exposure to a mixture of the perfluoroalkyl pesticide pyrifluquinazon and dibutyl phthalate disrupt male rat reproductive development in a dose additive manner.</td><td>115</td></tr><tr><td>Labels alimentaires et signes de qualité : promesses non tenues, une révision s'impose !</td><td>115</td></tr><tr><td>Bt cotton area contraction drives regional pest resurgence, crop loss and pesticide use</td><td>115</td></tr><tr><td>Glyphosate : 41 ONG demandent à la Commission européenne de mettre fin à l'utilisation d'études industrielles non fiables.</td><td>115</td></tr><tr><td>Weeds are now developing resistance to herbicides they've never been exposed to</td><td>116</td></tr><tr><td>Ag Secretary Vilsack pushes petroleum farming inputs, fights EU's climate-friendly organic "Food to Fork" Initiative</td><td>116</td></tr><tr><td>La « faim » serait-elle au bout de la Farm-to-Fork ?</td><td>116</td></tr><tr><td>Il est temps de moderniser les données européennes sur les pesticides.</td><td>116</td></tr><tr><td>Mesdames et messieurs les représentants des Etats membres, votez contre la ré-autorisation de l'herbicide Flumioxazine</td><td>116</td></tr><tr><td>Water contamination with pesticides goes unmonitored as problem escalates</td><td>117</td></tr><tr><td>Monoculture agriculture leads to poor soil health</td><td>117</td></tr><tr><td>Nouveau bras de fer entre l'interprofession et une association anti-phytos.</td><td>117</td></tr><tr><td>« France 2030 » Macron annonce 2 Mds d'€ dans des innovations de « rupture », dont la robotique.</td><td>117</td></tr><tr><td>Health risks posed by the use of mercury</td><td>118</td></tr><tr><td>Higher estimated pesticide exposures linked to ALS risk</td><td>118</td></tr><tr><td>Study adds to 40 year analysis linking brain cancer to pesticide exposure</td><td>118</td></tr><tr><td>La santé-environnement : un sujet au cœur d'un nouveau rapport tout à fait passionnant des inspections générales !</td><td>118</td></tr><tr><td>Glyphosate : de nombreuses études scientifiques manquantes dans le dossier d'évaluation</td><td>118</td></tr><tr><td>ONG : Encore une action en justice opportuniste contre l'État</td><td>119</td></tr><tr><td>Persistent organic pollutants, including banned pesticides, remain present in all fetal organs regardless of maternal chemical contamination</td><td>119</td></tr><tr><td>« Dénigrement des vins de Bordeaux » Appel à mobilisation</td><td>119</td></tr><tr><td>Studies show how pesticides harm organisms that form the foundation of freshwater ecosystems</td><td>119</td></tr><tr><td>Produits alimentaires : pour un affichage de leur impact environnemental</td><td>120</td></tr><tr><td>Lettre ouverte : pour une réorientation du Plan National de la PAC</td><td>120</td></tr><tr><td>More scientific evidence that endocrine-disrupting pesticides disrupt thyroid function.</td><td>120</td></tr><tr><td>Endocrine (Hormone) Disrupting Chemicals, Including Pesticides, Also Effect the Nervous System</td><td>120</td></tr><tr><td>Endangered Species Likely To Be Hard Hit by Neonicotinoid Insecticides, EPA Finds</td><td>121</td></tr></table>
REVUE DE PRESSE / RECHERCHE ET MEDIAS.................................................................................. 121

<table><tr><td>Nanoplastics found in the Alps were transported by air from Frankfurt, Paris and London</td><td>121</td></tr><tr><td>Upper ocean layer contains 24 trillion pieces of microplastics</td><td>121</td></tr><tr><td>Foetuses can be affected by microplastics, scientists find</td><td>121</td></tr><tr><td>Plastic pollution found in Taiwan's high mountain Jiaming Lake</td><td>121</td></tr><tr><td>Fish are being increasingly exposed to endocrine disrupters</td><td>122</td></tr><tr><td>Into the 'plastisphere': Scientists comb Japan waters to study new eco threat</td><td>122</td></tr><tr><td>Long-anticipated plan to regulate PFAS chemicals unveiled in US</td><td>122</td></tr><tr><td>Microplastics in belugas worked their way up food chain, researchers find</td><td>122</td></tr><tr><td>Mieux prévenir les résistances « Une information encore plus transparente » sur les étiquettes des phytos</td><td>123</td></tr><tr><td>Réchauffement climatique et pollution au plastique « sont interconnectés et nécessitent des solutions communes »</td><td>123</td></tr><tr><td>Les apiculteurs inquiets</td><td>123</td></tr><tr><td>Des rivières françaises polluées aux détergents, insecticides</td><td>123</td></tr><tr><td>How airborne microplastics affect climate change</td><td>123</td></tr><tr><td>Exposure to a mixture of PFOA and PFOS during pregnancy produces cumulative effects on F1 bodyweight, liver weight, and survival in the Sprague-Dawley rat</td><td>124</td></tr><tr><td>Managing and treating per- and polyfluoroalkyl substances (PFAS) in membrane concentrates</td><td>124</td></tr><tr><td>Les eurodéputés adoptent la Stratégie de « la ferme à la table »</td><td>124</td></tr><tr><td>Pollution au chlordécone : la collectivité territoriale de Martinique se porte partie civile</td><td>124</td></tr><tr><td>Opinion: The need for chemical simplification as a logical consequence of ever-increasing chemical pollution</td><td>124</td></tr><tr><td>L'intense lobbying de l'agro-industrie contre « Farm to Fork », le volet agricole du Pacte vert européen</td><td>125</td></tr><tr><td>Photos show Manila Bay mangroves 'choking' in plastic pollution</td><td>125</td></tr><tr><td>Protection des abeilles : les députés veulent en faire une « grande cause nationale » en 2022</td><td>125</td></tr><tr><td>Produits phytosanitaires : les CEPP peinent à être appliqués</td><td>125</td></tr><tr><td>Desulfonation and defluorination of 6:2 fluorotelomer sulfonic acid (6:2 FTSA) by Rhodococcus jostii RHA1: Carbon and sulfur sources, enzymes, and pathways</td><td>126</td></tr><tr><td>EPA announces updates on its efforts to address pfas in pesticide packaging</td><td>126</td></tr><tr><td>Bat guts become less healthy through diet of 'fast food' from banana plantations</td><td>126</td></tr><tr><td>Infants have more microplastics in their feces than adults, study finds</td><td>126</td></tr><tr><td>River research reveals scale of macroplastic pollution</td><td>126</td></tr><tr><td>Insecticide and climate warming impact stream insect communities</td><td>127</td></tr><tr><td>Ouverture au public du site Geod'air</td><td>127</td></tr><tr><td>Humpback whales as bioindicators of Antarctic mercury pollution</td><td>127</td></tr></table>
<table><tr><td>Data from 29,798 clean-ups around the world uncovers some of the worst litter hotspots</td><td>127</td></tr><tr><td>Bluefin tuna reveal global ocean patterns of mercury pollution</td><td>127</td></tr><tr><td>New report highlights link between air pollution and infant mortality</td><td>128</td></tr><tr><td>Sunlight can break down marine plastic into tens of thousands of chemical compounds</td><td>128</td></tr><tr><td>Définition des nanomatériaux : un petit pas vers une révision ?</td><td>128</td></tr><tr><td>First ever study finds microplastics in northeast Lapland</td><td>128</td></tr><tr><td>Nano/microparticles in conjunction with microalgae extract as novel insecticides against Mealworm beetles, Tenebrio molitor</td><td>128</td></tr><tr><td>Les viticulteurs pourront déroger à la réglementation sur les traitements à base de cuivre pour lutter contre le mildiou</td><td>129</td></tr></table>
# La méthode PICT (Pollution-Induced Community Tolerance), un outil complémentaire pour l'évaluation du risque et le biomonitoring des pesticides ?

## Introduction

Le concept du PICT – La méthode PICT (Pollution-Induced Community Tolerance) proposée par Blanck et al., (1988) est basée sur l'hypothèse qu'une communauté biologique préalablement soumise à la pression d'un toxique dans son environnement, sera plus tolérante à celui-ci. Une communauté biologique regroupe différents « composants », génotypes ou phénotypes, ayant une sensibilité différente vis-à-vis du toxique étudié. L'augmentation de la tolérance de la communauté résulte de plusieurs phénomènes induits par son exposition, dont l'adaptation et/ou l'acclimatation des populations, et/ou des changements dans la composition des espèces. Les organismes les plus sensibles, exposés au toxique à une concentration et pendant une durée suffisantes, sont directement éliminés ou ne sont plus concurrentiels et sont remplacés par des organismes plus tolérants, provoquant ainsi une succession induite par le toxique (TIS) (Blanck, 2002). La structure des peuplements est alors modifiée, et la communauté qui en résulte présente une tolérance supérieure au toxique, en comparaison à une communauté semblable mais n'ayant pas été préalablement exposée (témoin correspondant à la « ligne de base » de tolérance du milieu).

Concrètement, si l'on a le moyen de mesurer la sensibilité de cette communauté (préalablement exposée au toxique) à ce toxique, celle-ci devrait avoir une sensibilité moindre que celle attendue (i.e. celle des témoins). L'analyse de la diversité au sein de la communauté doit également montrer des modifications, pouvant conduire jusqu'à la disparition des taxons les plus sensibles.

Outre les intérêts de cette méthode pour étudier les processus écologiques et physiologiques de sélection et d'adaptation vis-à-vis d'un toxique, elle présente un atout fort pour l'évaluation du risque écotoxicologique à priori et à postériori : la mise en évidence de la relation de cause à effet entre l'exposition d'un contaminant dans le milieu (qu'il soit in situ ou dans le cadre d'expérimentations contrôlées) et les effets sur la communauté, intégrant la complexité structurelle et fonctionnelle du système étudié (Tlili et al., 2015).

Le PICT tient compte des différences de sensibilité des espèces et des interactions interspécifiques au sein de la communauté, alors que celles-ci ne sont pas prises en compte dans les tests monospécifiques (McClellan, et al., 2008).

Le principe du PICT est d'échantillonner une communauté préalablement exposée à un toxique de manière chronique in situ ou expérimentalement (phase de sélection), et de l'exposer à ce même toxique dans le cadre d'un bioessai contrôlé, afin d'en mesurer la sensibilité (courbe dose-réponse, CE50 ...), et de la comparer à celle d'une communauté comparable issue d'un milieu non contaminé considéré comme témoin (phase de détection). Parallèlement à ces mesures de sensibilité, les communautés comparées sont caractérisées par leur structure taxonomique (et/ou fonctionnelle).

Cette méthode a été appliquée dans divers contextes, marins, d'eaux douces, terrestres, principalement sur les communautés microbiennes (phyto et bactérioplancton, biofilms autotrophes et hétérotrophes, communautés microbiennes autotrophes et hétérotrophes de sédiments et de sols ...). Les microorganismes présentent des temps de génération courts, favorisant les études sur la sélection, et de nombreux protocoles éprouvés permettent de mesurer diverses activités globales à l'échelle des communautés, favorisant la pratique de bioessais pour évaluer la tolérance induite. De plus rares travaux impliquent des macroorganismes dans des études PICT (Millward et al., 1995, Courtney L.A. & Clements, 2000, Knopper et
al., 2002, Oguma et al., 2017), dont une étude très récente qui a mis en évidence un phénomène d'acquisition de tolérance de communautés zooplanctoniques exposées au glyphosate (Hébert et al., 2021).

## La Méthode (Fig.1)

1. **La phase de sélection** : au cours de laquelle la communauté biologique est soumise de manière chronique au toxique dans le milieu. C'est lors de cette phase que le contact entre le contaminant et la communauté doit être établi (biodisponibilité avérée), et que la durée d'exposition doit être suffisante pour induire un phénomène de sélection. Notons qu'en contexte expérimental (microcosmes, ou enclos), les paramètres de durée, d'application et de niveau d'exposition au toxique étudié sont contrôlés. Ces paramètres contrôlés ainsi que le choix entre des systèmes ouverts ou clos (permettant ou non les échanges d'organismes de la communauté étudiée avec l'extérieur) impacteront le processus de sélection et le rétablissement éventuel des communautés étudiées (Lambert et al., 2012, Blanck, 2002).

L'échantillonnage des organismes qui seront testés lors de la phase de détection est primordial. Il doit être le plus représentatif possible afin de saisir l'histoire de contamination chronique de la communauté étudiée. Pour les milieux aquatiques, différents systèmes de colonisation de biofilms sur des supports artificiels ont été développés (Blanck, 1985) : ici encore la durée de colonisation (15 jours à 3 semaines généralement) impacte cette phase de détection. Les échantillonnages des communautés aquatiques in situ peuvent poser le problème de l'ignorance de leur origine (en particulier pour le plancton susceptible de se déplacer avec les courants par exemple, mais la question se pose aussi pour les biofilms) et donc de leur exposition au toxique (Dorigo et al., 2004). L'échantillonnage des communautés microbiennes des sols et des sédiments nécessite parfois de passer par une phase d'extraction des organismes de la matrice complexe (Bérard et al., 2004), avec des biais de sélection possibles. Aucune méthode d'échantillonnage n'est totalement satisfaisante (constat général en écologie), il est important de prendre en compte ces éventuels biais dans l'interprétation des mesures lors de la phase de détection.

2. **La phase de détection** : au cours de laquelle la communauté biologique échantillonnée (ayant subi une exposition chronique préalable), est soumise de manière aigue au toxique incriminé dans le cadre de bioessais contrôlés. Le point final de mesure de l'effet toxique (endpoint) est généralement adapté au mode d'action du toxique incriminé et peut cibler certaines communautés microbiennes (exemple : phototrophes versus hétérotrophes). Divers endpoints ont été utilisés dans le cadre d'études PICT : en particulier l'incorporation du ¹⁴C (Blanck et al., 1988 Bérard et Benninghoff, 2001, Schmitt-Jansen et Altenburger, 2005) et la fluorescence in vivo (Seguin et al. 2002), la fluorimétrie PAM (Schmitt-Jansen et Altenburger, 2008, Magnuson et al., 2012), la synthèse des sulfolipides membranaires (Nyström et al., 2000), et des endpoints ciblant les communautés hétérotrophes tels que l'incorporation de la thymidine et de la leucine tritiées (Demoling et al., 2009), les éco-plaques (type Biolog, Rutger et al., 1999), la respiration microbienne (Dorigo et al., 2007, Tlili et al., 2011, Wakelin et al., 2014), les activités enzymatiques (Tlili et al., 2010, Bonnineau et al., 2013), l'oxydation potentielle de l'ammonium (Gong et al., 2002) ou du méthane (Seghers et al., 2003).

3. **La validation** : la validation de l'hypothèse PICT est généralement réalisée avec des caractérisations de la structure des communautés testées (structure taxonomique et/ou fonctionnelle), ainsi que par des mesures chimiques de la contamination des milieux étudiés. L'induction de tolérance par l'exposition des communautés aux polluants repose donc sur i) un historique de contamination préalable du milieu étudié (monde réel ou expérimental), ii) une modification de la structure des communautés (biodiversité et abondance des organismes) et iii) une diminution de la sensibilité à court terme lors d'une exposition expérimentale au(x) polluant(s).
<figure><img src="image_3.png" /></figure>

**Figure 1.** Les deux phases de l'approche PICT : sélection et détection. Durant la phase de sélection, des sélections inter- et intraspécifiques se produisent sous l'effet de l'exposition de la communauté (B) au toxique (restructuration de la communauté par la disparition des taxons sensibles et la dominance des taxons tolérants). Durant la phase de détection, la tolérance de la communauté échantillonnée est quantifiée en laboratoire au moyen de bioessais à court terme. Les réponses de paramètres fonctionnels (« endpoints ») aux concentrations croissantes du polluant testé sont mesurées, ce qui permet d'établir des courbes concentration-réponse pour la communauté « témoin » (A) et la communauté sélectionnée par le toxique (B). La tolérance est ensuite exprimée sous la forme d'une concentration effective (exemple EC50). La différence entre les valeurs ECx obtenues pour les deux communautés permet de quantifier le PICT. Parallèlement aux mesures de tolérance, la composition des deux communautés est évaluée et comparée pour valider l'hypothèse d'une sélection intra- et interspecifique. (Modifié d'après Tlili et al., 2015).

## Applications de la méthode PICT aux contaminations par les pesticides

Une requête bibliographique sur l'application du PICT aux pesticides et au cuivre (requête : (Pesticid* OR herbicid* OR cui OR copper) AND 'pollution-induced community tolerance OR PICT) AND (alga* OR Cyanob* OR periphyt* OR phytoplankt* OR liche* OR microbi*) = 116 résultats initiaux), ainsi que des articles connus par les experts, ont permis de sélectionner 68 études. Les premiers travaux sur le concept et la méthode PICT ont été élaborés par Blanck et al. (1988) en contexte de contamination par l'arsenic, les études PICT appliquées au PPP se sont développées à partir des années 2000 (avec un maximum de publications dans les années 2010).

Plus des 2/3 de ces études ont été réalisés en milieux aquatiques d'eau douce, seulement 13 % concernent les milieux marins et 20% les sols. 70% des études en milieux aquatiques ont pour modèle biologique les biofilms et le périphyton (en particulier en eau douce), les 30% des études restants sont principalement réalisés sur le plancton. Très peu d'études ont été réalisées sur les sédiments.
La majorité (53%) des toxiques étudiés sont les herbicides : principalement des inhibiteurs du PSII tels que les triazines (essentiellement atrazine, mais aussi prométryne, terbuthylazine, simazine), et des urées substituées (essentiellement le diuron et l'isoproturon) ; de rares études ont abordé la tolérance au 2,4-D (Zabaloy et al., 2010) et au glyphosate (Allegrini et al., 2015). Une seule étude à notre connaissance a abordé la tolérance vis-à-vis de métabolites d'herbicide (du diuron, Pesce et al., 2010). Les études sur le cuivre représentent plus de 43% des études PICT, il y a très peu d'études appliquées à la tolérance aux fongicides organiques (4%, tébuconazole et azoxystrobine).

C'est aussi en eaux douces que l'on retrouve la plus grande diversité de familles de molécules testées (en milieu marin, les biocides-antifouling ont aussi été étudiés, dont certains comme l'Irgarol sont des triazines, mais ne sont pas repris ici), alors que les études sur les sols sont aux 2/3 axées sur le cuivre (le tiers restant concerne les herbicides, nous n'avons pas trouvé d'études sur des fongicides organiques). Notons que dans les sols la méthode PICT est très majoritairement appliquée aux contaminations par les métaux (contaminations industrielles et liées aux épandages de déchets organiques, Brandt et al., 2010) et depuis les années 2010, à la tolérance aux antibiotiques en interaction avec ces contaminations métalliques (Berg et al., 2010, mais ces travaux ne sont pas repris ici). Si 80% des études sur sol ont été réalisées *in situ*, près des 2/3 des études aquatiques ont été réalisées en milieux contrôlés. On peut rapprocher cette constatation avec le peu de molécules testées : beaucoup d'études sont réalisées avec des molécules modèles (substances actives seules). Si ces molécules toxiques présentent une réalité de contamination (molécules retrouvées dans les sites étudiés par les laboratoires de recherche), elles sont aussi utilisées dans le cadre d'études en milieux contrôlés pour comprendre des processus écotoxicologiques de sélections/acquisition de tolérance et l'influence des variables du milieu, autres que les toxiques.

On peut citer l'exemple de l'observatoire de l'Ardières-Morcille (bassin versant viticole du Beaujolais), étudié depuis près de 20 ans (Gouy et al., 2021) et qui a permis de développer la méthode PICT en prenant comme molécules modèles deux phytosanitaires présents dans le bassin versant et la rivière : le diuron et le cuivre. Les premières études *in situ* ont permis de montrer les acquisitions de tolérance au diuron et au cuivre, associées aux changements de structure taxonomique des communautés périphytiques échantillonnées en amont et en aval de la rivière (Dorigo et al., 2007, Pesce et al. 2010), en établissant le lien de cause à effet de l'exposition. Des manipulations *in situ* de transplantation de périphyton de l'amont vers l'aval de la rivière ont confirmé ces acquisitions de tolérance, mais aussi des pertes de tolérance des communautés transplantées de l'aval vers l'amont, avec des réponses différentes selon le contaminant étudié (probablement liées au fait que les biofilms avaient initialement internalisé du cuivre et non du diuron). Cependant après 7 semaines d'acclimatation, la tolérance (envers le diuron et le cuivre) et la composition taxonomique des biofilms transplantés n'avaient toujours pas retrouvé les caractéristiques des communautés de l'amont de la rivière (Dorigo et al., 2010). Associé à ces mesures de contamination de l'eau et de transplantations sur le terrain, des expérimentations au laboratoire ont permis de préciser certains phénomènes associés à l'acquisition de tolérance et aux trajectoires de sélection (Tlili et al., 2010, Lambert et al., 2012). Les facteurs environnementaux (température) susceptibles d'impacter la tolérance des communautés aux contaminants (Lambert et al., 2017) et ceci de manière différente selon les communautés et leurs fonctions ont également été étudiés (Pesce et al., 2018). Enfin, le diuron ayant été interdit en 2008, une étude PICT *in situ* réalisée sur trois ans (2009-2011) à un rythme mensuel a permis de valider la méthode en conditions réelles, mettant en évidence la restauration des communautés périphytiques de la rivière (Pesce et al., 2016). Ce genre de résultats a été obtenu sur le lac Léman ayant connu une décroissance de la contamination pélagique par l'atrazine et le cuivre : des campagnes PICT ont été répétées mensuellement sur le phytoplancton durant une année à 12 ans d'intervalle (Larras et al. 2016).

Ces exemples soulignent l'intérêt de la méthode PICT comme outil de biomonitoring. Cependant, cet outil écotoxicologique, basé sur des processus écologiques et fonctionnels des communautés dans leurs milieux contaminés, n'est actuellement pas appliqué en routine pour l'évaluation du risque.
## Des verrous à débloquer

Pour pouvoir utiliser la méthode PICT dans le cadre d'évaluation du risque *a priori* et *a posteriori* induit par les pesticides, il est nécessaire de dépasser des verrous et de poursuivre un cadrage de la méthode (Tlili et al., 2015).

### La ligne de base et facteurs confondants

In situ, la ligne de base de tolérance (témoin) est généralement acquise sur une communauté prélevée dans une zone non (ou faiblement) contaminée par le toxique incriminé, mais comparable à la communauté exposée au toxique. Il est donc nécessaire d'échantillonner et de caractériser cette communauté témoin à chaque mesure PICT. Si l'objectif envisagé est de surveiller à plus large échelle une région selon ses contaminations en pesticides, trouver des sites témoins est complexe et définir une ligne de base plus générique devient un enjeu. Pour cela, il faut connaitre la variabilité de cette ligne de base de tolérance qui peut être influencée par diverses variables environnementales. Par exemple, une étude européenne sur la tolérance des biofilms de rivières vis-à-vis du zinc a mis en évidence de grandes variabilités de tolérance de base. Des outils de modélisation ont permis de caractériser les principaux paramètres impliqués dans cette variabilité, liés à la biodisponibilité du métal (Blanck et al., 2003). Une autre approche très récente, appliquée au sol, a montré avec des régressions multiples qu'il était possible de définir une ligne de base de tolérance au cuivre à partir d'une équation prenant en compte les caractéristiques physico-chimiques du sol et le type de matériau parental (Campillo-Cora et al., 2021). Cette équation dépend du contaminant étudié et de « l'endpoint » utilisé. Il n'existe pas à notre connaissance de travaux comparables sur des tentatives de définition de ligne de base pour estimer la tolérance induite par des contaminations organiques de type pesticides, mais les travaux expérimentaux et de suivis saisonniers in situ révélant certaines variables environnementales influençant cette tolérance de base (Pesce et al., 2018), montrent qu'il est possible d'aller dans cette direction. Dans ce sens, il est nécessaire de poursuivre en parallèle des travaux expérimentaux pour préciser les facteurs susceptibles d'être confondants dans l'évaluation de la tolérance (Blanck, 2002).

### Les mélanges de contaminants

Nous avons vu que la plupart des études PICT étaient focalisées sur un ou deux contaminants. Cependant, les milieux contaminés le sont généralement de manière multiple et non connue, et les pesticides présents dans le milieu sont accompagnés de leurs produits de dégradation. Par ailleurs, plusieurs études ont montré qu'il existait des phénomènes de cotolérance (un contaminant chronique pouvant induire une tolérance à un autre contaminant) entre différents contaminants et que les bioessais PICT ne pouvaient pas les discriminer : par exemple, Knauer et al. (2010), ont montré expérimentalement des phénomènes de cotolérance entre trois herbicides inhibiteurs du PSII, l'atrazine, le diuron et l'isoproturon avec des intensités différentes selon l'herbicide à l'origine de la pression de sélection. Une étude originale (Seghers et al., 2003) ciblée sur les méthanotrophes de sols contaminés par l'atrazine et le métolachlore (traitements sur 20 ans avec 0,75 kg et 2 kg respectivement /ha /an), a mis en évidence une tolérance accrue au 2,4-D (utilisé dans le bioessai PICT comme inhibiteur de l'oxydation du méthane par les microorganismes) de ces communautés préexposées à l'atrazine et au métolachlore, qui pourtant n'ont pas d'effets aigus à court terme (60 µg/ g sol) sur la capacité d'oxydation du méthane des communautés microbiennes.

Dans le cadre du suivi des milieux multicontaminés, on ne peut matériellement pas tester de nombreuses molécules avec l'outil PICT. En ce sens la cotolérance pourrait constituer un avantage, l'approche PICT pouvant être envisagée avec des molécules modèles de groupes chimiques sélectionnées sur la base de leurs propriétés de cotolérance. Ce type d'approche permettrait de renseigner l'exposition de l'écosystème vis-à-vis de classes de polluants. Pour cela il est nécessaire de connaitre les mécanismes induisant la cotolérance (molécules ayant le même mode d'action et/ou induisant des modes de détoxification comparables ?). C'est en multipliant les études dans divers contextes de contaminations pesticides, associant des mesures PICT à une surveillance chimique ; en développant des études expérimentales (normalisées), permettant de tester
diverses molécules ; et en associant ces observations à des approches écotoxiques in silico (Traoré et al., 2017), que nous pourrons faire avancer les connaissances sur cette question.

Une approche complémentaire proposée par Pesce et al. (2011) et Foulquier et al. (2015) est d'aborder la question de la multi contamination en testant directement ces mélanges réalistes dans les bioessais PICT. Des systèmes d'échantillonnage passif permettent de « récolter » la contamination du site et son historique, les solutions extraites obtenues sont d'une part, analysées chimiquement et d'autre part, testées sous forme de dilutions dans les bioessais appliqués aux communautés collectées dans le site impacté et dans le site témoin. Ce type d'approche permettrait d'établir la causalité entre l'exposition in situ à des mélanges complexes de micropolluants et les effets écotoxiques sur les communautés biologiques. Elle a été appliquée récemment avec succès dans le cadre de contaminations complexes de rivières en aval de stations d'épuration (Tlili et al., 2020). Ces deux approches associées permettraient d'augmenter la pertinence environnementale de la méthode PICT pour caractériser les milieux contaminés par les pesticides (Tlili et al., 2015).

La multi contamination interroge aussi sur le choix du(des) « endpoint(s) » de mesure de l'effet toxique à utiliser dans les bioessais PICT. Avoir un « endpoint » adapté au mode d'action du toxique incriminé est pertinent pour cibler un type de contaminant, mais il peut être limitant en contexte *in situ* de contaminations multiples et non connues (Blanck, 2002). Il est alors nécessaire de trouver un « endpoint » intégrateur de différents types de toxicité ou d'associer plusieurs « endpoints » pour tenir compte de la complexité à la fois des communautés étudiées, de la contamination chimique à laquelle elles ont été confrontées, et des impacts indirects possibles (Tlili et al., 2010, Tlili et al., 2020). Notons que Seghers et al. (2003) sont les seuls à notre connaissance à avoir « contourné » cette difficulté en utilisant dans leur bioessai PICT un herbicide un inhibiteur de l'oxydation du méthane (2,4-D) pour étudier la tolérance acquise de communautés ciblées (méthanotrophes) vis-à-vis d'autres types d'herbicides (Atrazine et métolachlore).

## Conclusion, perspectives

Si des travaux plus récents ont permis des avancées, les conclusions de Tlili et al. (2015) restent d'actualité :

-Standardiser les mesures PICT, notamment les échantillonnages des communautés testées, ainsi que les bioessais eux-mêmes (Lambert et al., 2015, Vazquez-Blanco et al., 2021).

-Travailler la question de la ligne de base à des échelles spatiales larges. Par exemple par écorégions en s'inspirant par exemple des travaux réalisés sur les bioindicateurs normalisés (tels que les indicateurs basés sur les diatomées de rivière, Tison et al., 2005).

-Poursuivre les développements de méthodes sur les mélanges complexes de contaminations (voir ci-dessus) et avec des familles récentes de substances actives.

-Développer une plus grande variété (et stabiliser la standardisation) d'« endpoints » représentatifs des fonctions et des structures des écosystèmes microbiens. Dans ce sens, les avancées « omics » (Mahamoud Ahmed et al., 2020) et de chémotaxonomie devraient permettre de préciser nos connaissances sur les processus d'acquisition de tolérance et les acteurs de ces acquisitions et contribuer à analyser la présence de gènes participant à la résistance et à la tolérance des communautés.

Peu d'outils existent ayant à la fois une pertinence écologique et écotoxicologique permettant d'établir le lien de causalité entre une contamination et la santé d'un écosystème. La méthode PICT reste un outil prometteur, et ces dix dernières années ont montré qu'il était utilisable dans le cadre d'expérimentations et de biomonitoring, y compris dans des milieux soumis à des contaminations complexes. L'enjeu de poursuivre son amélioration et de dépasser les verrous actuels afin d'en faire un outil opérationnel d'évaluation du risque des pesticides est fort.
## Contacts

Annette Bérard¹, Joan Artigas², Christophe Leboulanger³, Soizic Morin⁴, Christian Mougin⁵, Stéphane Pesce⁶, Sabine Stachowski-Haberkorn⁷

¹UMR 1114 EMMAH, INRAE/Avignon Université, Domaine Saint Paul, 228 Route de l'Aérodrome, 84914 Avignon Cedex 9

²UMR 6023 LMGE, CNRS/UCA, Campus Universitaire des Cézeaux, 1 Impasse Amélie Murat, 63178 Aubière

³UMR Marbec, Université de Montpellier/IRD/Ifremer/CNRS, Avenue Jean Monnet, 34200 Sète

⁴UR EABX, INRAE, 50 avenue de Verdun, 33612 Cestas cedex

⁵UMR 1402 ECOSYS, Université Paris-Saclay/INRAE/AgroParisTech, Route de Saint-Cyr, 78026 Versailles cedex

⁶UR RiverLy, INRAE, 5 Rue de la Doua, 69625 Villeurbanne Cedex

⁷Laboratoire Physiologie et Biotechnologie des Algues, Ifremer, Rue de l'Ile d'Yeu, 44311 Nantes Cedex 03

<figure><img src="image_4.png" /></figure>

Expertise Scientifique Collective
Phytopharmaceutiques
Biodiversité
Services Ecosystémiques

INRAE
la science pour la vie, l'humain, la terre

<figure><img src="image_5.png" /></figure>

Ifremer

## Pour en savoir plus

Cette fiche thématique a été rédigée dans le cadre de l'expertise collective « Phytopharmaceutiques Biodiversité Services Ecosystémiques ». Vous pouvez consulter concernant cette ESCo :

-La fiche thématique : https://www6.inrae.fr/ecotox/Productions/Fiches-thematiques/Fiche-thematique-N-29-octobre-2020

-La publication : Pesce, S., Mamy, L., Achard, A.L. et al. Collective scientific assessment as a relevant tool to inform public debate and policymaking: an illustration about the effects of plant protection products on biodiversity and ecosystem services. Environ Sci Pollut Res 28, 38448-38454 (2021). https://doi.org/10.1007/s11356-021-14863-w

A lire également : https://en.wikipedia.org/wiki/Pollution-induced步community步tolerance

## Bibliographie citée

Allegrini, Marco, Zabaloy, María Celina, Gómez, Elena del V., 2015. Ecotoxicological assessment of soil microbial community tolerance to glyphosate. Science of the Total Environment 533: 60-68.

Bérard A., Benninghoff C., 2001. Pollution-Induced Community Tolerance (PICT) and seasonal variations in the sensitivity of phytoplankton to atrazine in microcosms. Chemosphere Vol. 45 (4-5): 427-437.

Bérard, A., Rimet, F., Capowiez, Y., Leboulanger, C. 2004. Procedures for Determining the Pesticide Sensitivity of Indigenous Soil Algae: A Possible Bioindicator of Soil Contamination?. Archives of Environmental Contamination and Toxicology 46(1): 24-31.

Blanck H. 2002. A critical review of procedures and approaches used for assessing pollution-induced community tolerance (PICT) in biotic communities. Human and Ecological Risk Assessment 8: 1003-1034.

Blanck, H., Admiraal, W., Cleven, R. F. M. J., Guasch, H., van den Hoop, M. A. G. T., Ivorra, N., Nyström, B., Paulsson, M., Petterson, R. P., Sabater, S., Tubbing, G. M. J., 2003. "Variability in Zinc Tolerance, Measured as Incorporation of Radio-Labeled Carbon Dioxide and Thymidine, in Periphyton Communities Sampled from 15 European River Stretches". Archives of Environmental Contamination and Toxicology 44(1): 17-29. doi:10.1007/s00244-002-1258-4

Blanck, Hans, Wangberg, S. A., Molander, S., 1988. "Pollution-Induced Community Tolerance – A New Ecotoxicological Tool." Functional Testing of Aquatic Biota for Estimating Hazards of Chemicals". American Society for Testing and Materials. STP 988: 219-230

Blanck, H., 1985. A simple, community level, ecotoxicological test system using samples of periphyton. Hydrobiologia 124: 251-261
Brandt KK., Frandsen RJN., Holm PE., Nybroe O., 2010. Development of pollution-induced community tolerance is linked to structural and functional resilience of a soil bacterial community following a five-year field exposure to copper. Soil Biology & Biochemistry 42: 748-757.

Bonnineau, C., Tili, A., Faggiano, L., Montuelle, B., Guasch, H., 2013. The use of antioxidant enzymes in freshwater biofilms: Temporal variability vs. toxicological responses. Aquatic Toxicology 136-137: 60-71. doi:10.1016/j.aquatox.2013.03.009. ISSN 0166-445X.

Campillo-Cora, C., Soto-Gomez, D., Arias-Estévez, M., Baath, E., 2021. Bacterial community tolerance to Cu in soils with geochemical baseline concentrations (GBCs) of heavy metals: Importance for pollution induced community tolerance (PICT) determinations using the leucine incorporation method. Soil Biology and Biochemistry 155: 108157. doi:10.1016/j.soilbio.2021.108157. ISSN 0038-0717

Courtney L.A., Clements W.H., 2000. Sensitivity to acidic pH in benthic invertebrate assemblages with different histories of exposure to metals. Journal of the North American Benthological Society, 19, 112-127.

Demoling, LA., Bååth, E., Greve, G., Wouterse, M., Schmitt, H., 2009. Effects of sulfamethoxazole on soil microbial communities after adding substrate. Soil Biology and Biochemistry 41(4): 840-848. doi:10.1016/j.soilbio.2009.02.001. ISSN 0038-0717.

Dorigo U., Bourrain X, Berard A, Leboulanger C, 2004. Seasonal changes in the sensitivity of river microalgae to atrazine and isoproturon along a contamination gradient. Sci. Total Environ, 318: 101-114.

Dorigo, U, Leboulanger, C, Bérard, A, Bouchez, A, Humbert, JF, Montuelle, B., 2007. Lotic biofilm community structure and pesticide tolerance along a contamination gradient in a vineyard area. Aquatic Microbial Ecology 50: 91-102. doi:10.3354/ame01133. ISSN 0948-3055.

Dorigo, U., Berard, A., Rimet, F., Bouchez, A., Montuelle, B., 2010. In situ assessment of periphyton recovery in a river contaminated by pesticides. Aquatic Toxicology 98(4): 396-406. doi:10.1016/j.aquatox.2010.03.011. ISSN 0166-445X

Foulquier, A., Morin, S., Dabrin, A., Margoum, C., Mazzella, N., Pesce, S., 2015. Effects of mixtures of dissolved and particulate contaminants on phototrophic biofilms: new insights from a PICT approach combining toxicity tests with passive samplers and model substances. Environmental Science and Pollution Research 22(6): 4025-4036. doi:10.1007/s11356-014-3289-6. ISSN 0944-1344.

Gong, P., Siciliano, S. D., Srivastava, S., Greer, CW., Sunahara, GL., 2002. Assessment of Pollution-Induced Microbial Community Tolerance to Heavy Metals in Soil Using Ammonia-Oxidizing Bacteria and Biolog Assay. Human and Ecological Risk Assessment: An International Journal 8(5): 1067-1081. doi:10.1080/1080-700291905828. ISSN 1080-7039.

Gouy, V., Liger, L., Ahrouch, S., Bonnineau, C., Carluer, N., Chaumot, A., Coquery, M., Dabrin, A., Margoum, C., & Pesce, S. 2021. Ardières-Morcille in the Beaujolais, France: A research catchment dedicated to study of the transport and impacts of diffuse agricultural pollution in rivers. Hydrological Processes 35(10), e14384. https://doi.org/10.1002/hyp.14384

Hébert M-P, Fugère V, Beisner BE, da Costa NB, Barrett RDH, Bell G, Shapiro BJ, Yargeau V Gonzalez A, Fussmann GF, 2021. Widespread agrochemicals differentially affect zooplankton biomass and community structure. Ecological Applications (in press) doi: 10.1002/EAP.2423

Knauer, Katja, Leimgruber, Andrea, Hommen, Udo, Knauert, Stefanie (2010). Co-tolerance of phytoplankton communities to photosynthesis II inhibitors. Aquatic Toxicology 96 (4): 256-263. doi:10.1016/j.aquatox.2009.11.001.

Knopper, L. D., Siciliano, S. D. (2002). A Hypothetical Application of the Pollution-Induced Community Tolerance Concept in Megafaunal Communities Found at Contaminated Sites". Human and Ecological Risk Assessment: An International Journal 8(5): 1057-1066. doi:10.1080/1080-700291905819. ISSN 1080-7039.

Lambert, AS., Dabrin, A., Foulquier, A., Morin, S., Rosy, C., Coquery, M., Pesce, S., 2017. Influence of temperature in pollution-induced community tolerance approaches used to assess effects of copper on freshwater phototrophic periphyton. Science of the Total Environment 607-608: 1018-1025. doi:10.1016/j.scitotenv.2017.07.035.

Lambert, AS., Pesce, S., Foulquier, A., Gahou, J., Coquery, M., Dabrin, A., 2015. Improved short-term toxicity test protocol to assess metal tolerance in phototrophic periphyton: toward standardization of PICT approaches. Environmental Science and Pollution Research 22(6): 4037-4045. doi:10.1007/s11356-014-3505-4. ISSN 0944-1344

Lambert, AS., Morin, S., Artigas, J., Volat, B., Coquery, M., Neyra, M., Pesce, S., 2012. Structural and functional recovery of microbial biofilms after a decrease in copper exposure: Influence of the presence of pristine communities. Aquatic Toxicology 109: 118-126. doi:10.1016/j.aquatox.2011.12.006.

Larras, F., Rimet, F., Gregorio, V., Bérard, A., Leboulanger, C., Montuelle, B., Bouchez, A., 2016. Pollution-induced community tolerance (PICT) as a tool for monitoring Lake Geneva long-term in situ ecotoxic restoration from herbicide contamination. Environmental Science and Pollution Research 23(5): 4301-4311. doi:10.1007/s11356-015-5302-0

Magnusson M, Heimann, K, Ridd, M, Negri, AP, 2012. Chronic herbicide exposures affect the sensitivity and community structure of tropical benthic microalgae. Marine Pollution Bulletin 65(4-9): 363-372. DOI 10.1016/j.marpolbul.2011.09.029

Mahamoud AA., Tardy, V., Bonnineau, C., Billard, P., Pesce, S., Lyautey, E., 2020. Changes in sediment microbial diversity following chronic copper-exposure induce community copper-tolerance without increasing sensitivity to arsenic. Journal of Hazardous Materials 391: 122197. doi:10.1016/j.jhazmat.2020.122197.

McClellan K., Altenburger R. & Schmitt-Jansen M. (2008) Pollution-induced community tolerance as a measure of species interaction in toxicity assessment. Journal of Applied Ecology 45: 1514-1522.

Millward, RN., Grant, A., 1995. Assessing the impact of copper on nematode communities from a chronically metal-enriched estuary using pollution-induced community tolerance. Marine Pollution Bulletin 30 (11): 701-706. doi:10.1016/0025-326x(95)00053-p. ISSN 0025-326X.

Nyström et al., 2000. Evaluation of the capacity for development of atrazine tolerance in periphyton from a Swedish freshwater site as determined by inhibition of photosynthesis and sulfolipid synthesis. Environmental Toxicology and Chemistry 19(5): 1324-1331, doi: 10.1002/etc.5620190515

Oguma, AY., Klerks, PL., 2017. Pollution-induced community tolerance in benthic macroinvertebrates of a mildly lead-contaminated lake. Environmental Science and Pollution Research 24 (23): 19076-19085. doi:10.1007/s11356-017-9553-9. ISSN 0944-1344.

Pesce S, Lissalde S, Lavieille D, Margoum C, Mazzella N, Roubeix V, Montuelle B, 2010. Evaluation of single and joint toxic effects of diuron and its main metabolites on natural phototrophic biofilms using a pollution-induced community tolerance (PICT) approach. Aquatic Toxicology 99: 492-499

Pesce, S., Margoum C., Foulquier A., 2016. Pollution-induced community tolerance for in situ assessment of recovery in river microbial communities following the ban of the herbicide diuron. Agriculture, Ecosystems & Environment 221: 79-86. doi:10.1016/j.agee.2016.01.009. ISSN 0167-8809.

Pesce, S., Lambert, AS., Morin, S., Foulquier, A., Coquery, M., Dabrin, A., 2018. Experimental Warming Differentially Influences the Vulnerability of Phototrophic and Heterotrophic Periphytic Communities to Copper Toxicity. Frontiers in Microbiology 9: 1424. doi:10.3389/fmicb.2018.01424. ISSN 1664-302X. PMC 6036129. PMID 30013533.
Pesce, S., Margoum C., Montuelle, B., 2010. In situ relationships between spatio-temporal variations in diuron concentrations and phototrophic biofilm tolerance in a contaminated river. Water Research 44(6): 1941-1949. doi:10.1016/j.watres.2009.11.053.

Pesce, S., Morin, S., Lissalde, S., Montuelle, B., Mazzella, N., 2011. Combining polar organic chemical integrative samplers (POCIS) with toxicity testing to evaluate pesticide mixture effects on natural phototrophic biofilms. Environmental Pollution 159 (3): 735-741. doi:10.1016/j.envpol.2010.11.034

Rutgers, M., Breure, AM., 1999. Risk Assessment, Microbial Communities, and Pollution-Induced Community Tolerance. Human and Ecological Risk Assessment: An International Journal 5(4): 661-670. doi:10.1080/10807039.1999.9657730

Schmitt-Jansen, M., Altenburger, R., 2005. Predicting and observing responses of algal communities to photosystem II-herbicide exposure using pollution-induced community tolerance and species-sensitivity distributions. Environmental Toxicology and Chemistry 24(2): 304-312. doi:10.1897/03-647.1

Schmitt-Jansen, M., Altenburger, R., 2008. Community-level microalgal toxicity assessment by multiwavelength-excitation PAM fluorometry. Aquatic Toxicology 86(1): 49-58. doi:10.1016/j.aquatox.2007.10.001

Seghers D., et al., 2003. Pollution induced community tolerance (PICT) and analysis of 16S rRNA genes to evaluate the long-term effects of herbicides on methanotrophic communities in soil. European Journal of Soil Science 54: 679-684. doi: 10.1046/j.1365-2389.2003.00560.x

Seguin, F, Le Bihan, F, Leboulanger, C, Bérard, A, 2002. A risk assessment of pollution: induction of atrazine tolerance in phytoplankton communities in freshwater outdoor mesocosms, using chlorophyll fluorescence as an endpoint. Water Research 36(13): 3227-3236. doi:10.1016/s0043-1354(02)00013-1

Tison, J., Park, Y.-S., Coste, M., Wasson, J.G., Ector, L., Rimet, F., Delmas, F., 2005. Typology of diatom communities and the influence of hydro-ecoregions: A study on the French hydrosystem scale. Water Research 39: 3177-3188, https://doi.org/10.1016/j.watres.2005.05.029.

Tili A, Bérard A, Blanck H, Bouchez A, Cassio F, Eriksson Km, Morin S, Montuelle B, Navarro E, Pascoal C, Pesce S, Schmitt-Jansen M, Behra R, 2015, Pollution-induced community tolerance (PICT): towards an ecologically relevant risk assessment of chemicals in aquatic systems, Freswater Biol online: 8 APR 2015. doi:10.1111/fwb.12558. http://dx.doi.org/10.1111/fwb.12558

Tili A., Berard A, Roulier J-L, Volat B., Montuelle B, 2010. PO₄ dependence of biofilm autotrophic and heterotrophic communities tolerance to copper and diuron. Aquatic Toxicol. doi:10.1016/j.aquatox.2010.02.008

Tili A., Marechal M., Montuelle B Volat B., Dorigo U., Bérard A., 2011. Use of the Microresp method to assess Pollution-Induced Community Tolerance for lotic biofilms. Environ Pollut 159(1): 18-24. doi:10.1016/j.envpol.2010.09.033

Tili, A., Corcoll, N., Arrhenius, A., Backhaus, T., Hollender, J., Creusot, N., Wagner, B., Behra, R., 2020. Tolerance Patterns in Stream Biofilms Link Complex Chemical Pollution to Ecological Impacts. Environmental Science & Technology 54(17): 10745-10753. doi:10.1021/acs.est.0c02975

Traoré, H., Crouzet, O., Mamy, L., Sireyjol, C., Rossard, V., Servien, R., Latrille, E., Martin-Laurent, F., Patureau, D., Benoit, P., 2017. Clustering pesticides according to their molecular properties, fate, and effects by considering additional ecotoxicological parameters in the TyPol method. Environmental Science and Pollution Research 25(5): 4728-4738. doi:10.1007/s11356-017-0758-8

Vázquez-Blanco, R., Arias-Estévez, M., Bààth, E., Fernández-Calviño, D., 2021. Comparing the effect of Cu-based fungicides and pure Cu salts on microbial biomass, microbial community structure and bacterial community tolerance to Cu. Journal of Hazardous Material 409: 124960. doi:10.1016/j.jhazmat.2020.124960.

Wakelin, S, Gerard E., Black A., Hamonts K., Condron L., Yuan T., van Nostrand J., Zhou J., O'Callaghan M., 2014. Mechanisms of pollution induced community tolerance in a soil microbial community exposed to Cu. Environmental Pollution 190: 1-9. doi:10.1016/j.envpol.2014.03.008. ISSN 0269-7491

Zabaloy, MC., Garland, JL., Gomez, MA., 2010. Assessment of the impact of 2,4-dichlorophenoxyacetic acid (2,4-D) on indigenous herbicide-degrading bacteria and microbial community function in an agricultural soil. Applied Soil Ecology 46(2): 240-246. doi:10.1016/j.apsoil.2010.08.00
ERA / PUBLICATIONS

SCIENTIFIQUES /

COMMUNAUTES

MICROBIENNES

AQUATIQUES

Detection of the maximum
resistance to the herbicides
diuron and glyphosate, and
evaluation of its phenotypic
cost, in freshwater
phytoplankton

Authors: Melero-Jimenez IJ, Banares-Espana E, Reul A et al.

Source: AQUATIC TOXICOLOGY 240: 105973, 2021, DOI 10.1016/j.aquatox.2021.105973

**Abstract:** One of the most important anthropogenic impacts on freshwater aquatic ecosystems close to intensive agriculture areas is the cumulative increase in herbicide concentrations. The threat is especially relevant for phytoplankton organisms because they have the same physiological targets as the plants for which herbicides have been designed. This led us to explore the evolutionary response of three phytoplanktonic species to increasing concentrations of two herbicides and its consequences in terms of growth and photosynthesis performance. Specifically, we used an experimental ratchet protocol to investigate the differential evolution and the limit of resistance of a cyanobacterium (Microcystis aeruginosa) and two chlorophyceans (Chlamydomonas reinhardtii and Dictyosphaerium chlorelloides) to two herbicides in worldwide use: glyphosate and diuron...

Microalgae Growth Inhibition-
Based Reservoirs Water
Quality Assessment to
Identify Ecotoxicological Risks

Authors: Rodrigues S, Pinto I, Formigo N, Antunes SC

Source: WATER 13: 2605, 2021, DOI 10.3390/w13192605

**Abstract:** This work intended to assess the adaptability of bioassay with Raphidocelis subcapitata to be used as a complement to the water quality assessment parameters of reservoirs imposed by the European Water Framework Directive (WFD). Thus, water samples of Portuguese reservoirs (Miranda, Pocinho, Agueira, and Alqueva) were analyzed in three sampling periods (spring and autumn 2019, and spring 2020). A physical and chemical report of waters was also performed...

Toxicity assessment and
underlying mechanisms of
multiple metal organic
frameworks using the green
algae *Chlamydomonas
reinhardtii* model

Authors: Li YL, Shang SS, Shang J, Wang WX

Source: ENVIRONMENTAL POLLUTION 291: 118199, 2021, DOI 10.1016/j.envpol.2021.118199

**Abstract:** Metal-organic frameworks (MOFs) are an emerging class of materials which have garnered increasing attention for their utility as adsorbents and photocatalysts in water treatment. Nevertheless, the environmental risks of MOFs, especially their underlying impacts on aquatic organisms, are not fully explored. Herein, the toxicity of multiple representative MOFs was systematically assessed using a freshwater green alga (*Chlamydomonas reinhardtii*) model...
# Sediments alleviate the inhibition effects of antibiotics on denitrification: Functional gene, microbial community, and antibiotic resistance gene analysis

**Authors:** Zhang RJ, Xu XM, Jia DT et al.

**Source:** SCIENCE OF THE TOTAL ENVIRONMENT
804: 150092, 2022, DOI
10.1016/j.scitotenv.2021.150092

**Abstract:** Both antibiotics and sediments can affect the denitrification in aquatic systems. However, little is known how antibiotics influence the denitrification in the presence of sediments. Here, the effects of antibiotics (sulfamethoxazole, tetracycline and ofloxacin) on denitrification in the absence and presence of sediments were investigated. The influencing mechanisms were revealed by quantifying the denitrification functional genes, 16S-seq of bacteria, and antibiotic resistance genes...

# System biology analysis of endosulfan biodegradation in bacteria and its effect in other living systems: modeling and simulation studies

**Authors:** Bhandari G, Sharma M, Negi S et al.

**Source:** JOURNAL OF BIOMOLECULAR STRUCTURE
& DYNAMICS Early Access, DOI
10.1080/07391102.2021.1982773

**Abstract:** Endosulfan is a broadly applied cyclodiene insecticide which has been in use across 80 countries since last 5 decades. Owing to its recalcitrant nature, endosulfan residues have been reported from air, water and soil causing toxicity to various non-target organisms. Microbial decontamination of endosulfan has been reported previously by several authors. In the current study, we have evaluated the pathways of endosulfan degradation and its hazardous impact on other living beings including insects, humans, plants, aquatic life and environment by in-silico methods. For establishment of the endosulfan

metabolism in different ecosystems, cell designer was employed. The established model was thereafter assessed and simulated to understand the biochemical and physiological metabolism of the endosulfan in various systems of the network...

# Translocation of Epipelic Biofilms and Their Short-Term Responses to Urbanization Impacts in Nutrient Rich Streams

**Authors:** Cochero J, Gelis MMN, Donadelli J, Gomez N

**Source:** ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS 93: e20210379, 2021, DOI 10.1590/0001-3765202120210379

**Abstract:** Stream biofilms are among the first to react to environmental degradation, since their structural and functional characteristics are tightly linked to the physicochemical variables in the water and sediment. The objectives of this research were to study the differences in chlorophyll-a, bacterial density and metabolism endpoints of epipelic biofilms in nutrient-rich streams under different physical-chemical conditions in the stream water in relation to changes in urbanization, and to measure the short-term responses (up to 72 h) in the biofilm when translocated to more urbanized sites. For these purposes, chlorophyll-a, bacterial density, biofilm respiration (electron transfer activity) and O-2 consumption were measured in epipelic biofilms in nutrient-rich streams exposed to different levels of urbanization after a 30 day colonization. Afterward, biofilms were translocated downstream to more polluted sites, and sampled to identify any fast occurring changes to be considered as potential indicators of environmental degradation...

# The toxicity of cadmium-copper mixtures on daphnids and microalgae analyzed using the Biotic Ligand Model

**Authors:** Clement B, Felix V, Bertrand V
**Source:** ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH Early Access, DOI 10.1007/s11356-021-16516-4

**Abstract:** For the prediction of metal mixture ecotoxicity, the BLM approach is promising since it evaluates the amount of metals accumulated on the biotic ligand on the basis of water chemistry, i.e., species (major cations) competing with metals, and related toxicity. Based on previous work by Farley et al. (2015) (MMME research project), this study aimed at modeling toxicity of Cd:Cu mixtures to the crustacean Daphnia magna (48-h immobilization tests) and the microalga Pseudokirchneriella subcapitata (72-h growth inhibition tests). The 2012 version of the USGS model was chosen, assuming additivity of effects and accumulation of metals on a single site. The assumption that EDTA could contribute to toxicity through metals complexing was also tested, and potential effects due to reduction of ions Ca2+ absorption by metals were considered...

## The response of sediment microbial communities to temporal and site-specific variations of pollution in interconnected aquaculture pond and ditch systems

**Authors:** Xu M, Xu RZ, Shen XX et al.

**Source:** SCIENCE OF THE TOTAL ENVIRONMENT 806: 150498, 2022, DOI 10.1016/j.scitotenv.2021.150498

**Abstract:** Sediment microbial communities play critical roles in the health of fish and the biogeochemical cycling of elements in aquaculture ecosystems. However, the response of microbial communities to temporal and spatial variations in interconnected aquaculture pond and ditch systems remains unclear. In this study, 61 sediment bacterial samples were collected over one year from 11 sites (including five ponds and six ditches) in a 30-year-old fish aquaculture farm. The 16S rRNA approach was used to determine the relative abundances of microbial communities in the sediment samples. The relationships among nutrients, heavy metals, and abundant microorganisms were analyzed...

## Tylosin toxicity in the alga Raphidocelis subcapitata revealed by integrated analyses of transcriptome and metabolome: Photosynthesis and DNA replication-coupled repair

**Authors:** Li Q, Lu DL, Sun HT et al.

**Source:** AQUATIC TOXICOLOGY 239: 105964, 2021, DOI 10.1016/j.aquatox.2021.105964

**Abstract:** Tylosin (TYN) is widely used in veterinary prophylactic as a macrolide and frequently detected in the surface water. Previous studies showed that exposure to TYN caused suppression of chlorophyll biosynthesis and inhibition of photosynthesis at the physiological level, associated with reduced growth performances in algae, but the molecular mechanisms remain unknown, especially at environmental exposure levels. The present study elucidated the underlying molecular mechanism(s) of TYN toxicity in a model green alga Raphidocelis subcapitata using approaches of transcriptomics and metabolomics...

## Gut microbiome alterations in the crustacean Pacifastacus leniusculus exposed to environmental concentrations of antibiotics and effects on susceptibility to bacteria challenges

**Authors:** Hernandez-Perez A, Zamora-Briseno JA, Soderhall K, Soderhall I

**Source:** DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 126: 104181, 2022, DOI 10.1016/j.dci.2021.104181

**Abstract:** Gut-associated microbiota in crustaceans are recognized as a key element for maintaining homeostasis and health in the animal. Since the richness of these microbial communities is strongly influenced by the local environment, especially in aquatic organisms, it is important to address to what extent environmental variations
can affect these communities. In the present study, we used high-throughput 16S rRNA sequencing technology to study the composition of gut-associated microbiota of the crayfish Pacifastacus leniusculus after exposure to environmentally-relevant concentrations of an antibiotic, namely sulfamethoxazole. Also, we examined if alterations of microbiota caused by environmentally-relevant concentrations of this antibiotic affected the host susceptibility to bacterial diseases, including Vibrio species...

## Responses of benthic diatoms to waters affected by post-fire contamination

**Authors:** Vidal T, Pereira JL, Moreira F et al.

**Source:** SCIENCE OF THE TOTAL ENVIRONMENT
800: 49473, 2021, DOI
10.1016/j.scitotenv.2021.149473

**Abstract:** Wildfire effects go beyond direct impact in terrestrial ecosystems. Specifically, the periphytic communities of aquatic ecosystems standing within and downstream the burnt areas are relevant ecological receptors of post-fire runoff contamination. Nevertheless, the off-site impacts of wildfires in these communities are limitedly studied so far. The present study aimed to assess the effects of river water contaminated with ash loaded runoff in the growth benthic diatom Navicula libonensis (Schoeman 1970). Four surface water samples were collected approximately one year after the wildfire for laboratory testing with the diatom: one was collected from a site upstream the burnt area, within the Unhais river (UU); three were collected from sites standing within the burnt area, one in the Unhais river (UB) and two in the Zezere river (Z1 and Z2), reflecting different hydrological regimes...

## Ecological thresholds of periphytic communities and ecosystems integrity in lower Doce River basin

**Authors:** Zorzal-Almeida S, Fernandes VD

**Source:** SCIENCE OF THE TOTAL ENVIRONMENT
796: 148965, 2021, DOI
10.1016/j.scitotenv.2021.148965

**Abstract:** Freshwater biodiversity has been impacted by several stressors such as eutrophication, turbidity and metals. Besides these frequent impacts, large-scale accidents occasionally affect aquatic systems, input an intense load of contaminants to the water bodies, as in the case of the Fundao tailing dam collapse (Brazil), which launched mil-lions of meters cubic of iron ore tailing in the Doce River Basin. Our aim in this study was to assess how much stress the environmental conditions of lentic and lotic environments in the lower region of this basin impact the periphytic community. In addition, we intend to verify whether the limits of changes of the periphytic communities agreed with Brazilian legislation for the protection of aquatic life. For that, monthly samplings were carried out in seven sampling stations in lentic environments and five in lotic environments, including the Doce River channel, between October/2018 and March/2021 (except in October/2019) ...

## Terrestrial input of herbicides has significant impacts on phytoplankton and bacterioplankton communities in coastal waters

**Authors:** Yang LQ, Mou SL, Li HM et al.

**Source:** LIMNOLOGY AND OCEANOGRAPHY Early Access, 2021, DOI 10.1002/lno.11940

**Abstract:** Herbicide use has increased very rapidly worldwide in recent decades, and some of these herbicides eventually enter the ocean. At present, herbicide pollution in the ocean has become a global environmental problem. Here, in a field investigation of 22 commonly used herbicide residues (triazine, phenylurea, and amide) in the Bohai Sea and the Yellow Sea, we found that the detection rate of herbicide residues within 100 km from shore was 100%. [...] Under exposure to triazine herbicides at close to their total in situ concentrations, the photosynthesis of the phytoplankton community was significantly inhibited, [...] the phytoplankton community structure underwent significant changes with the dominant group shifting from diatoms to dinoflagellates, due to the higher atrazine sensitivity of diatoms compared to facultative dinoflagellates...
Photoactive titanium dioxide
nanoparticles modify
heterotrophic microbial
functioning

**Authors:** Bundschuh M, Zubrod JP, Konschak M et al.

**Source:** ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH 28: 49550-49558, 2021, DOI 10.1007/s11356-021-14090-3

**Abstract:** Nanoparticulate titanium dioxide (nTiO(2)) is frequently applied, raising concerns about potential side effects on the environment. While various studies have assessed structural effects in aquatic model ecosystems, its impact on ecosystem functions provided by microbial communities (biofilms) is not well understood. This is all the more the case when considering additional stressors, such as UV irradiation - a factor known to amplify nTiO (2)-induced toxicity. Using pairwise comparisons, we assessed the impact of UV (UV-A = 1.6 W/m (2); UV-B = 0.7 W/m (2)) at 0, 20 or 2000 µg nTiO (2)/L on two ecosystem functions provided by leaf-associated biofilms...

Artificial Intelligence Meets
Marine Ecotoxicology:
Applying Deep Learning to
Bio-Optical Data from Marine
Diatoms Exposed to Legacy
and Emerging Contaminants

**Authors:** Rodrigues NM, Batista JE, Mariano P et al.

**Source:** BIOLOGY-BASEL 10: 932, 2021, DOI 10.3390/biology10090932

**Abstract:** Our work is motivated by the increasing production of chemicals with environmentally harmful effects to our aquatic ecosystems. We show that it is possible to detect and distinguish the presence of several different emerging contaminants, using the photochemical responses of a microalgae species, which is among the most abundant phytoplankton group in the oceans. We use several machine learning and deep learning models that operate on chlorophyll fluorescence induction curves, which are composed of

fluorescence values taken at different time steps from the microalgae exposure trials...

Micro-polyethylene particles
reduce the toxicity of nano
zinc oxide in marine
microalgae by adsorption

**Authors:** Li JJ, Mao S, Ye YY et al.

**Source:** ENVIRONMENTAL POLLUTION 290: 118042, 2021, DOI 10.1016/j.envpol.2021.118042

**Abstract:** Contaminant adsorption by microplastics (MPs) allows them to act as contaminant vehicles or vectors, complicating eco-toxicological study of MPs. The contaminants adsorbed are mainly organic contaminants, especially hydrophobic organic contaminants (HOCs), although heavy-metal adsorption has also been reported. Compared to the mechanisms of HOC adsorption, those for metals are not fully understood. In the present study, combined-exposure assays revealed that polyethylene microplastics (PEMPs, 150 μm) alleviate the toxic effect of nano zinc oxide (nZnO, 20-30 nm) on marine microalgal growth by 14.4%. Thus, we hypothesized that nZnO adsorption onto PEMP surfaces ameliorates its toxicity to microorganisms. To test this hypothesis, PEMP samples isolated from nZnO suspensions were characterized. Their surfaces were observed by SEM, their Zn levels were measured by ICP-MS, and the compound form of Zn on the PEMP surface was determined by XRD analysis...

Taxonomic and Functional
Distribution of Bacterial
Communities in Domestic and
Hospital Wastewater System:
Implications for Public and
Environmental Health

**Authors:** Selvarajan R, Sibanda T, Pandian J, Mearns K

**Source:** ANTIBIOTICS-BASEL 10: 1059, 2021, DOI 10.3390/antibiotics10091059

**Abstract:** The discharge of untreated hospital and domestic wastewater into receiving water bodies
is still a prevalent practice in developing countries. Unfortunately, because of an ever-increasing population of people who are perennially under medication, these wastewaters contain residues of antibiotics and other antimicrobials as well as microbial shedding, the direct and indirect effects of which include the dissemination of antibiotic resistance genes and an increase in the evolution of antibiotic-resistant bacteria that pose a threat to public and environmental health. This study assessed the taxonomic and functional profiles of bacterial communities, as well as the antibiotic concentrations in untreated domestic wastewater (DWW) and hospital wastewater (HWW), using high-throughput sequencing analysis and solid-phase extraction coupled to Ultra-high-performance liquid chromatography Mass Spectrometry (UHPLC-MS/MS) analysis, respectively. The physicochemical qualities of both wastewater systems were also determined...

## Impacts of municipal wastewater treatment plant discharge on microbial community structure and function of the receiving river in Northwest Tibetan Plateau

Authors: Wang JW, Chen Y, Cai PG et al.

Source: JOURNAL OF HAZARDOUS MATERIALS 423: 127170, 2022, DOI 10.1016/j.jhazmat.2021.127170

Abstract: Wastewater treatment plant (WWTP) effluents carrying plenty of nutrients and micropollutants pose serious threats to receiving rivers, however, the response of microbial community structure and function to WWTP effluents discharge is still poorly understood. To address this knowledge gap, paired water and sediment samples from 17 sites of the Huangshui River, and effluents from 6 WWTPs were collected to investigate the effect of WWTP discharge on riverine microbial communities...

## Medium composition affects the heavy metal tolerance of microalgae: a comparison

Authors: Rathnayake IVN, Megharaj M, Beer M, Naidu R

Source: JOURNAL OF APPLIED PHYCOLOGY Early Access, DOI 10.1007/s10811-021-02589-8

Abstract: Tolerance of the three metals cadmium (Cd), copper (Cu), and zinc (Zn) by four microalgal species was investigated in three different culture media available in the literature together with a modified version in order to study the effect of growth media components in estimating the bioavailability of metals introduced into the medium. The free metal content of each medium was also determined using Visual MINTEQ version 3.1 to compare the bioassays. Four microalgal isolates were identified as Desmodesmus sp-I, Desmodesmus sp-II, Coelastrella sp., and Chlorella vulgaris...

## New insights into mechanisms of copper nanoparticle toxicity in freshwater algae *Chlamydomonas reinhardtii*: Effects on the pathways of secondary metabolites

Authors: Janova A, Kolackova M, Bytesnikova Z et al.

Source: ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 60: 102476, 2021, DOI 10.1016/j.algal.2021.102476

Abstract: The effects of copper nanoparticles (Cu-NPs), including their stability in the medium, were studied with the green unicellular algae *Chlamydomonas reinhardtii* (CC-125). Cu-NPs were synthesized and characterized. Cu-NP particles were uniform, regular, and largely spherical, and they had smooth surfaces; the average size was estimated to be 137.4 +/- 2.1 nm. Chlamydomonas cells were cultivated for 96 h under controlled conditions in the presence of Cu-NPs, according to OECD guidelines, and then subjected to toxicological bioassays [...] Attention was also paid to select underexplored metabolites, which were studied with a LC-MS/MS system...
Single and Combined Effects
of Cypermethrin and UVR Pre-
Exposure in the Microalgae
*Phaeodactylum Tricornutum*

**Authors:** Cabrera J, Marcoval MA, Diaz-Jaramillo M, Gonzalez M

**Source:** ARCHIVES OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY 81: 507-516, 2021, DOI 10.1007/s00244-021-00889-1

**Abstract:** Coastal marine microalgae are exposed to anthropogenic pollutants, including pesticides from aquaculture/agriculture/household uses. Some microalgae species, such as *Phaeodactylum tricornutum*, can induce and accumulate UV-absorbing compounds (UACs) upon ultraviolet radiation (UVR) exposure to prevent deleterious effects. Tolerance mechanisms activated by natural stressors might also protect organisms from anthropogenic stressors. This work assesses the effects of the insecticide cypermethrin (Cyp) and UVR in the marine microalgae *P tricornutum*. Considering the pro-oxidant properties of both stressors and UACs' induction in *P tricornutum*, lethal and sublethal effects of Cyp were tested in cultures with and without UVR acclimation. After a 24-h exposure to 10 µg L-1 of technical Cyp or culture medium, UACs, growth, glutathione-S-transferase activity (GST), sulfhydryl groups (SH-g), and lipid peroxidation (LPO) were analyzed...

Impact of wastewater on the
microbial diversity of
periphyton and its tolerance
to micropollutants in an
engineered flow-through
channel system

Authors: Carles L, Wullschleger S, Joss A et al.

**Source:** WATER RESEARCH 203: 117486, 2021, DOI 10.1016/j.watres.2021.117486

**Abstract:** Wastewater treatment plants (WWTPs) play an important role in retaining organic matter and nutrients but to a lesser extent micropollutants. Therefore, treated wastewater is recognized as a major source of multiple stressors, including complex mixtures of micropollutants. These can potentially affect

microbial communities in the receiving water
bodies and the ecological functions they provide.
In this study, we evaluated in flow-through
channels the consequences of an exposure to a
mixture of stream water and different
percentages of urban WWTP effluent, ranging
from 0% to 80%, on the microbial diversity and
function of periphyton communities. Assuming
that micropollutants exert a selective pressure for
tolerant microorganisms within communities, we
further examined the periphyton sensitivity to a
micropollutant mixture extracted from passive
samplers that were immersed in the wastewater
effluent. As well, micropollutants in water and in
periphyton were comprehensively quantified...

Toxicities of three metal
oxide nanoparticles to a
marine microalga: Impacts on
the motility and potential
affecting mechanisms

Authors: Du XY, Zhou WS, Zhang WX et al.

**Source:** ENVIRONMENTAL POLLUTION 290: 118027, 2021, DOI 10.1016/j.envpol.2021.118027

**Abstract:** With the fast growth of the production and application of engineered nanomaterials (ENMs), nanoparticles (NPs) that escape into the environment have drawn increasing attention due to their ecotoxicological impacts. Motile microalgae are a type of primary producer in most ecosystems; however, the impacts of NPs on the motility of microalgae have not been studied yet. So the toxic impacts of three common metal oxide NPs (nTiO(2), nZnO, and nFe(2)O(3)) on swimming speed and locomotion mode of a marine microalgae, Platymonas subcordiformis, were investigated in this study...

Microbial diversity alteration
reveals biomarkers of
contamination in soil-river-
lake continuum

Authors: Bourhane Z, Lanzen A, Cagnon C et al.

**Source:** JOURNAL OF HAZARDOUS MATERIALS 421: 126789, 2022, DOI 10.1016/j.jhazmat.2021.126789
**Abstract:** Microbial communities inhabiting soil-water-sediment continuum in coastal areas provide important ecosystem services. Their adaptation in response to environmental stressors, particularly mitigating the impact of pollutants discharged from human activities, has been considered for the development of microbial biomonitoring tools, but their use is still in the infancy. Here, chemical and molecular (16S rRNA gene metabarcoding) approaches were combined in order to determine the impact of pollutants on microbial assemblages inhabiting the aquatic network of a soil-water-sediment continuum around the Ichkeul Lake (Tunisia), an area highly impacted by human activities. Samples were collected within the soil-river-lake continuum at three stations in dry (summer) and wet (winter) seasons...

## Temperature, phytoplankton density and bacteria diversity drive the biotransformation of micropollutants in a lake ecosystem

**Authors:** Chalifour A, Walser JC, Pomati F, Fenner K

**Source:** WATER RESEARCH 202: 117412, 2021, DOI 10.1016/j.watres.2021.117412

**Abstract:** For most micropollutants (MPs) present in surface waters, such as pesticides and pharmaceuticals, the contribution of biotransformation to their overall removal from lake ecosystems is largely unknown. This study aims at empirically determining the biotransformation rate constants for 35 MPs at different periods of the year and depths of a meso-eutrophic lake. We then tested statistically the association of environmental parameters and microbial community composition with the biotransformation rate constants obtained...

## A review on catalytic-enzyme degradation of toxic environmental pollutants: Microbial enzymes

**Authors:** Saravanan A, Kumar PS, Vo DVN et al.

**Source:** JOURNAL OF HAZARDOUS MATERIALS 419: 126451, 2021, DOI 10.1016/j.jhazmat.2021.126451

**Abstract:** Industrialization and other human anthropogenic activities cause serious threats to the environment. The toxic pollutants can cause detrimental diseases on diverse living beings in their respective ecosystems. Bioremediation is one of the efficient remediation methods in which the toxic pollutants are removed from the environment by the application of microorganisms or their biologically active products (enzymes). Typically, the microorganisms in the environment produce various enzymes to immobilize and degrade the toxic environmental pollutants by utilizing them as a substrate for their growth and development. Both the bacterial and fungal enzymes can degrade the toxic pollutants present in the environment and convert them into non-toxic forms through their catalytic reaction mechanism. [...] This review focused on enzymatic removal of toxic pollutants such as heavy metals, dyes, plastics and pesticides in the environment...

## The effects of copper ions and copper nanomaterials on the output of amino acids from marine microalgae

**Authors:** Huang WQ, Zhou YP, Zhao T et al.

**Source:** ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH Early Access, DOI 10.1007/s11356-021-16347-3

**Abstract:** In this study, the marine microalgae Skeletonema costatum and Nitzschia closterium were exposed to different forms of copper, such as a metal salt (Cu2+), a nano-metal (nano-Cu), and nano-metal oxide (nano-CuO). During a 96-h exposure to nanoparticles (NPs) and salt, the cell number, Cu2+ concentration in the culture medium, morphology, and intracellular amino acids were measured to assess the toxicity of the copper materials and the toxicity mechanism of the NPs...
# Bacterial survival strategies and responses under heavy metal stress: a comprehensive overview

**Authors:** Pal A, Bhattacharjee S, Saha J et al.

**Source:** CRITICAL REVIEWS IN MICROBIOLOGY
Early Access, DOI 10.1080/1040841X.2021.1970512

**Abstract:** Heavy metals bring long-term hazardous consequences and pose a serious threat to all life forms. Being non-biodegradable, they can remain in the food webs for a long period of time. Metal ions are essential for life and indispensable for almost all aspects of metabolism but can be toxic beyond threshold level to all living beings including microbes. Heavy metals are generally present in the environment, but many geogenic and anthropogenic activities has led to excess metal ion accumulation in the environment. To survive in harsh metal contaminated environments, bacteria have certain resistance mechanisms to metabolize and transform heavy metals into less hazardous forms. This also gives rise to different species of heavy metal resistant bacteria. Herein, we have tried to incorporate the different aspects of heavy metal toxicity in bacteria and provide an up-to-date and across-the-board review. The various aspects of heavy metal biology of bacteria encompassed in this review includes the biological notion of heavy metals, toxic effect of heavy metals on bacteria, the factors regulating bacterial heavy metal resistance, the diverse mechanisms governing bacterial heavy metal resistance, bacterial responses to heavy metal stress, and a brief overview of gene regulation under heavy metal stress...

# BactoTraits - A functional trait database to evaluate how natural and man-induced changes influence the assembly of bacterial communities

**Authors:** Cebron A, Zeghal E, Usseglio-Polatera P et al.

**Source:** ECOLOGICAL INDICATORS 130:108047, 2021, DOI10.1016/j.ecolind.2021.108047

**Abstract:** In the environment, abiotic (climatic conditions, physico-chemical parameters), biotic (interactions between microorganisms, vegetation and fauna), and anthropogenic (stress, pollution) filters are driving the microbial diversity observed locally. A key question in microbial ecology is to understand the impact of these filters on bacterial diversity and ecosystem functioning. To highlight the responses of bacterial assemblages to these ecological filters, a new approach based on bacterial functional traits has been developed. This approach provides a functional picture of bacterial assemblages using morphological, physiological, and genomic traits as proxies of functions, and leads to a generalizable approach over a larger range of ecosystems with different bacterial diversities. We have created a user-friendly database of bacterial functional traits, thanks to the properties of 19,455 bacterial strains. This database has been called BactoTraits. [...] As an example of application, BactoTraits was used to characterize the traits and functional diversity of bacterial assemblages in soil samples from 10 sites with different physico-chemical properties and various levels of metal and polycyclic aromatic hydrocarbon (PAH) contaminations...

# Odiel River (SW Spain), a Singular Scenario Affected by Acid Mine Drainage (AMD): Graphical and Statistical Models to Assess Diatoms and Water Hydrogeochemistry Interactions

**Authors:** Grande JA, Luis AT, Cordoba F et al.

**Source:** INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH 18: 8454, 2021, DOI 10.3390/ijerph18168454

**Abstract:** The Odiel River (SW Spain) is one of the most cited rivers in the scientific literature due to its high pollution degree, generated by more than 80 sulphide mines' (mostly unrestored) contamination in the Iberian Pyritic Belt (IPB), that have been exploited for more than 5000 years. Along the river and its tributaries, the physico-chemical parameters and diatoms, from
15 sampling points, were analyzed in the laboratory. Physico-chemical parameters, water chemical analysis, together with richness and Shannon-Wiener indexes were integrated in a matrix. An initial graphical treatment allowed the definition and proposal of a functioning system model, as well as the establishment of cause-effect relationships between pollution and its effects on biota. Then, the proposed model was statistically validated by factor analysis...

## Intraspecific variation in metal tolerance modulate competition between two marine diatoms

**Authors:** Andersson B, Godhe A, Filipsson HL et al.

**Source:** ISME JOURNAL Early Access, DOI 10.1038/s41396-021-01092-9

**Abstract:** Despite widespread metal pollution of coastal ecosystems, little is known of its effect on marine phytoplankton. We designed a co-cultivation experiment to test if toxic dose-response relationships can be used to predict the competitive outcome of two species under metal stress. Specifically, we took into account intraspecific strain variation and selection. We used 72 h dose-response relationships to model how silver (Ag), cadmium (Cd), and copper (Cu) affect both intraspecific strain selection and competition between taxa in two marine diatoms (Skeletonema marinoi and Thalassiosira baltica). The models were validated against 10-day co-culture experiments, using four strains per species...

## Marine biofilms on different fouling control coating types reveal differences in microbial community composition and abundance

**Authors:** Papadatou M, Robson SC, Dobretsov S et al.

**Source:** MICROBIOLOGYOPEN 10: e1231, 2021, DOI 10.1002/mbo3.1231

**Abstract:** Marine biofouling imposes serious environmental and economic impacts on marine

applications, especially in the shipping industry. To combat biofouling, protective coatings are applied on vessel hulls which are divided into two major groups: biocidal and non-toxic fouling release. The current study aimed to explore the effect of coating type on microbial biofilm community profiles to better understand the differences between the communities developed on fouling control biocidal antifouling and biocidal-free coatings. Biocidal (Intersmooth (R) 7460HS SPC), fouling release (Intersleek (R) 900), and inert surfaces were deployed in the marine environment for 4 months, and the biofilms that developed on these surfaces were investigated using Illumina NGS sequencing, targeting the prokaryotic 16S rRNA gene...

## Benthic microbial diversity trends in response to heavy metals in an oxygen-deficient eutrophic bay of the Humboldt current system offshore the Atacama Desert

**Authors:** Zarate A, Dorador C, Valds J et al.

**Source:** ENVIRONMENTAL POLLUTION 286:117281, 2021, DOI 10.1016/j.envpol.2021.117281

**Abstract:** Mejillones Bay is a coastal ecosystem situated in an oxygen-deficient upwelling area impacted by mining activities in the coastal desert region of northern Chile, where conspicuous microbial life develops in the sediments. Herein, heavy metal (loid)s (HMs) such as Cu, Pb, As, Zn, Al, Fe, Cd, Mo, Ni and V as well as benthic microbial communities were studied using spectrometry and iTag-16 S rRNA sequencing. Samples were taken from two contrasting sedimentary localities in the Bay named Punta Rieles (PR) and Punta Chacaya (PC) within 10-50 m water-depth gradient...

## Effect of heavy metals on protein content of marine unicellular green alga *Dunaliella tertiolecta*

**Authors:** El Agawany N, Kaamoush M, El-Zeiny A, Ahmed M
**Source:** ENVIRONMENTAL MONITORING AND ASSESSMENT 193: 584, 2021, DOI 10.1007/s10661-021-09353-y

**Abstract:** Microalgae are rich source of protein containing necessary amino acids at different levels. The present study was designed to assess stimulatory and/or inhibitory impact of five different concentrations (5, 10, 15, 20, and 25 mg/L) of three essential heavy metals (nickel, zinc, and copper) on protein content (soluble, insoluble, and total) of the marine unicellular green alga *Dunaliella tertiolecta*. Further, geospatial analyses were used to assess the suitability of Qaroun Lake for *D. tertiolecta* proliferation...

Environmentally relevant fungicide levels modify fungal community composition and interactions but not functioning

Authors: Baudy P, Zubrod JP, Konschak M et al.

**Source:** ENVIRONMENTAL POLLUTION 285:117234, 2021, DOI 10.1016/j.envpol.2021.117234

**Abstract:** Aquatic hyphomycetes (AHs), a group of saprotrophic fungi adapted to submerged leaf litter, play key functional roles in stream ecosystems as decomposers and food source for higher trophic levels. Fungicides, controlling fungal pathogens, target evolutionary conserved molecular processes in fungi and contaminate streams via their use in agricultural and urban landscapes. Thus fungicides pose a risk to AHs and the functions they provide. To investigate the impacts of fungicide exposure on the composition and functioning of AH communities, we exposed four AH species in monocultures and mixed cultures to increasing fungicide concentrations (0, 5, 50, 500, and 2500 mg/L). We assessed the biomass of each species via quantitative real-time PCR. Moreover, leaf decomposition was investigated...

Specific toxicity of azithromycin to the freshwater microalga *Raphidocelis subcapitata*

Authors: Almeida AC, Gomes T, Lomba JAB, Lillicrap A

**Source:** ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 222:112553, 2021, DOI 10.1016/j.ecoenv.2021.112553

**Abstract:** Pharmaceuticals are produced to inflict a specific physiological response in organisms. However, as only partially metabolized after administration, these types of compounds can also originate harmful side effects to non-target organisms. Additionally, there is still a lack of knowledge on the toxicological effects of legacy pharmaceuticals such as the antibiotic azithromycin. This macrolide occurs at high concentrations in the aquatic environment and can constitute a threat to aquatic organisms that are at the basis of the aquatic food chain, namely microalgae. This study established a high-throughput methodology to study the toxicity of azithromycin to the freshwater microalga *Raphidocelis subcapitata*. Flow cytometry and pulse amplitude modulated (PAM) fluorometry were used as screening tools...

ERA / PUBLICATIONS
SCIENTIFIQUES / ECOTOXICOLOGIE
SPATIALE

High spatial resolution measurements of passive-sampler derived air concentrations of persistent organic pollutants in the Campania region, Italy: Implications for source identification and risk analysis

Authors: Qu C, De Vivo B, Albanese S, Fortelli A et al.

**Source:** Environmental Pollution 286: 117248, 2021, https://doi.org/10.1016/j.envpol.2021.117248
**Abstract:** Because most relevant studies have used small sample sizes, to date, representative atmospheric monitoring of persistent organic pollutants (POPs) on a regional scale has been very limited, which makes it difficult to precisely identify "hotspots" and possible pollution sources. In this study, an ultrahigh resolution monitoring technique was used to measure the atmospheric spatial variations in POP concentrations on a regional scale, throughout Campania, Italy...

## Application of land use regression modelling to describe atmospheric levels of semivolatile organic compounds on a national scale

**Authors:** White KB, Sáňka O, Melymuk L, Přibylová P et al.

**Source:** Science of The Total Environment 148520, 2021, https://doi.org/10.1016/j.scitotenv.2021.148520

**Abstract:** Despite the success of passive sampler-based monitoring networks in capturing global atmospheric distributions of semivolatile organic compounds (SVOCs), their limited spatial resolution remains a challenge. Adequate spatial coverage is necessary to better characterize concentration gradients, identify point sources, estimate human exposure, and evaluate the effectiveness of chemical regulations such as the Stockholm Convention on Persistent Organic Pollutants. Land use regression (LUR) modelling can be used to integrate land use characteristics and other predictor variables (industrial emissions, traffic intensity, demographics, etc.) to describe or predict the distribution of air concentrations at unmeasured locations across a region or country...

## Occurrence and risk assessment of pesticides in a Mediterranean Basin with strong agricultural pressure

## (Guadiana Basin: Southern of Portugal)

**Authors:** Palma P, Fialho S, Lima A, Catarino A et al.

**Source:** Science of the Total Environment 794: 148703, 2021, https://doi.org/10.1016/j.scitotenv.2021.148703

**Abstract:** The study aimed to assess the occurrence and the environmental risk of a group of 51 selected pesticides in the Guadiana Basin (a biodiversity hotspot, in the Mediterranean).

The site-specific risk assessment showed a spatial and temporal pattern, with a higher risk occurring mainly in intermittent streams, in the drought period...

## A spatially explicit model for estimating risks of pesticide exposure to bird populations

**Authors:** Etterson M, Schumaker N, Garber K, Lennartz S et al.

**Source:** PLOS ONE 16(6): e0252545, 2021, https://doi.org/10.1371/journal.pone.0252545

**Abstract:** Pesticides are used widely in agriculture and have the potential to affect non-target organisms, including birds. We developed an integrated modeling system to allow for spatially-explicit evaluation of potential impacts to bird populations following exposures to pesticides...

## Supporting non-target arthropods in agroecosystems: Modelling effects of insecticides and landscape structure on carabids in agricultural landscapes

**Authors:** Ziółkowska E, Topping CJ, Bednarska AJ, & Laskowski R

**Source:** Science of the Total Environment 774: 145746, 2021, https://doi.org/10.1016/j.scitotenv.2021.145746
**Abstract:** Intensification of agricultural practices is one of the most important drivers of the dramatic decline of arthropod species. We do not know, however, the relative contribution to decline of different anthropogenic stressors that are part of this process. We used high-resolution dynamic landscape models and advanced spatially-explicit population modelling to estimate the relative importance of insecticide use and landscape structure for population dynamics of a widespread carabid beetle *Bembidion lampros*. The effects of in-crop mitigation measures through the application of insecticides with reduced lethality, and off-crop mitigation measures by increasing abundance of grassy field margins, were evaluated for the beetle along the gradient of landscape heterogeneity...

Conservation risks and benefits of establishing monarch butterfly (*Danaus plexippus*) breeding habitats close to maize and soybean fields in the north central United States: A landscape-scale analysis of the impact of foliar insecticide on nonmigratory monarch butterfly populations

Authors: Grant TJ, Krishnan N, & Bradbury SP

Source: Integrated Environmental Assessment and Management 17(5): 989-1002, 2021, DOI 10.1002/ieam.4402

**Abstract:** Establishing habitat in agricultural landscapes of the north central United States is critical to reversing the decline of North America's eastern monarch butterfly (*Danaus plexippus*) population. Insecticide use could create population sinks and threaten recovery. Discouraging habitat establishment within a 38-m zone around crop fields is a suggested risk mitigation measure. In Story County, Iowa, United States, this mitigation would discourage habitat establishment in 84% of roadsides and 38% of noncrop land. It is unclear if the conservation benefits from establishing habitat close to crop

fields outweigh suppression of population growth owing to insecticide exposure. Consequently, monarch conservation plans require spatially and temporally explicit landscape-scale assessments...

A Simplified Population-Level Landscape Model Identifying Ecological Risk Drivers of Pesticide Applications, Part One: Case Study for Large Herbivorous Mammals

Authors: Tarazona D, Tarazona G & Tarazona JV

Source: Int. J. Environ. Res. Public Health 18(15): 7720, 2021, https://doi.org/10.3390/ijerph18157720

**Abstract:** Environmental risk assessment is a key process for the authorization of pesticides, and is subjected to continuous challenges and updates. Current approaches are based on standard scenarios and independent substance-crop assessments. This arrangement does not address the complexity of agricultural ecosystems with mammals feeding on different crops. This work presents a simplified model for regulatory use addressing landscape variability, co-exposure to several pesticides, and predicting the effect on population abundance. The focus is on terrestrial vertebrates and the aim is the identification of the key risk drivers impacting on mid-term population dynamics...

Natural habitat partially mitigates negative pesticide effects on tropical pollinator communities

Authors: Obregon D, Guerrero OR, Stashenko E & Poveda K

Source: Global Ecology and Conservation e01668, 2021, https://doi.org/10.1016/j.gecco.2021.e01668

**Abstract:** Land-use change and pesticides have been identified as two of the main causes behind pollinator decline. Understanding how these factors affect crop pollinator communities is crucial to inform practices that generate optimal
pollination and ensure sustainable food production. In this study, we investigated the effects of landscape composition and pesticide residues on bee communities and their pollination services in *Solanum quitoense* Lam. "lulo" crops in Colombia...

## The Role of Source-Sink Dynamics in the Assessment of Risk to Nontarget Arthropods from the Use of Plant Protection Products

**Authors:** Lewis G, Dinter A, Elston C, Marx MT et al.

**Source:** Environmental Toxicology and Chemistry 40(10): 2667-2679, 2021, https://doi.org/10.1002/etc.5137

**Abstract:** The concept of source-sink dynamics as a potentially important component of metapopulation dynamics was introduced in the 1980s. The objective of the present review was to review the considerable body of work that has been developed, to consider its theoretical implications as well as to understand how source-sink dynamics may manifest under field conditions in the specific case of nontarget arthropods in the agricultural environment...

We highlight the importance of considering the spatial and temporal heterogeneity of agricultural landscapes when assessing the population dynamics of nontarget arthropods in the context of the risk from the use of plant protection products...

## Identities, concentrations, and sources of pesticide exposure in pollen collected by managed bees during blueberry pollination

**Authors:** Graham KK, Milbrath MO, Zhang Y, Soehnlen A et al.

**Source:** Scientific Reports 11(1): 1-13, 2021, https://doi.org/10.1038/s41598-021-96249-z

**Abstract:** Bees are critical for crop pollination, but there is limited information on levels and

sources of pesticide exposure in commercial agriculture. We collected pollen from foraging honey bees and bumble bees returning to colonies placed in blooming blueberry fields with different management approaches (conventional, organic, unmanaged) and located across different landscape settings to determine how these factors affect pesticide exposure. We also identified the pollen and analyzed whether pesticide exposure was correlated with corbicular load composition...

## ERA / PUBLICATIONS

### SCIENTIFIQUES /

### MICROBIOLOGIE ET

### CONTAMINANTS

## Interactions between heavy metals and bacteria in mangroves

**Authors:** Meng SS, Peng T, Pratush A et al.

**Source:** Marine Pollution Bulletin 172: 2021, DOI 10.1016/j.marpolbul.2021.112846

**Abstract:** Environmental heavy metal pollution has become a serious problem in recent years. Therefore, our study investigated seven heavy metal-contaminated mangroves (Beihai, Fangchenggang, Hainan, Hongkong, Shenzhen, Yunxiao, and Zhanjiang) in southern China, and found that they were particularly polluted with Zn and Pb. These heavy metals were mainly distributed in the surface sediments of the mangroves...

## A direct contact bioassay using sulfur-oxidizing bacteria (SOB) for toxicity assessment of contaminated field soils

**Authors:** Ashun E, Toor UA, Kim HS et al.

**Source:** Chemosphere 286: 1, 2022, DOI 10.1016/j.chemosphere.2021.131599
**Abstract:** In this study, 11 low/uncontaminated (including Lufa 2.2) and 9 contaminated field soils with varying geophysical and physicochemical characteristics were evaluated for toxicities based on oxygen consumption of sulfur-oxidizing bacteria (SOB)...

## Fatty acid signatures of sediment microbial community in the chronically polluted mangrove ecosystem

**Authors:** Semanti P, Robin RS, Purvaja R, Ramesh R

**Source:** Marine Pollution Bulletin 172, 2021, DOI 10.1016/j.marpolbul.2021.112885

**Abstract:** Phospholipid fatty acid (PLFA) analysis was used to examine variation in the distribution of microbial communities in heavily polluted mangrove sediments of Thane creek, west coast of India. A total of 40 individual PLFAs representing 11 functional groups were identified in the sediment and were mainly dominated by saturated fatty acids (anaerobic prokaryotes) 1%) were detected in the sediment indicating their sensitivity to anthropic stressors...

## Microbial Metal Resistance within Structured Environments Is Inversely Related to Environmental Pore Size

**Authors:** Harvey HJ, Mitzakoff AMT, Wildman RD and more...

**Source:** Applied and Environmental Microbiology 87: 20, 2021, DOI 10.1128/AEM.01005-21

**Abstract:** The physical environments in which microorganisms naturally reside rarely have homogeneous structure, and changes in their porous architecture may have effects on microbial activities that are not typically captured in conventional laboratory studies. In this study, to investigate the influence of environmental structure on microbial responses to stress, we constructed structured environments with different pore properties (determined by X-ray computed tomography)...

## Detection of metals and associated bacteria from Mumbai mangroves and their impact analysis

**Authors:** Banerjee S, Ghosh S, Singh K and more...

**Source:** Regional Studies in Marine Science 8, 2021, DOI 10.1016/j.rsma.2021.102007

**Abstract:** The distribution of Pb, Ni, Zn, Cu, Cr, Mn, S, P, Na, Ca, K, Si, Al, Fe were studied in the mangrove sediments across five different sites in Maharashtra. Energy dispersive X-ray fluorescence method was utilized for the determination of the elements in the sediment both before and after the monsoon. The spatial variation, as well as the monsoon was seen to affect the metal concentration in the mangroves. A total of 34 and 9 bacterial isolates were isolated pre and post-monsoon respectively. The metal tolerance index of the microbes was increased...

## Effects of environmental factors on soil bacterial community structure and diversity in different contaminated districts of Southwest China mine tailings

**Authors:** Wu BH, Luo HY, Wang XT and more...

**Source:** Science of the Total Environment 802, 2022, DOI 10.1016/j.scitotenv.2021.149899

**Abstract:** A mass of tailings left by mineral exploitation have caused serious environmental pollution. Although many studies have shown that soil microorganisms have the potential to remediate environmental pollution, the interaction mechanism between microorganisms and the surrounding environment of tailings is still unclear. In this study, 15 samples around pyrite mine tailing were collected to explore the ecological effects of environmental factors on bacterial community...
## Nano zero-valent iron-induced changes in soil iron species and soil bacterial communities contribute to the fate of Cd

**Authors:** Liu MJ, Wang J, Xu M and more...

**Source:** Journal of Hazardous Materials 424, A, 2021, DOI 10.1016/j.jhazmat.2021.127343

**Abstract:** Nano zero-valent iron (nZVI) is used for soil remediation; however, the impact of nZVI on soil solid iron phases and its interactions with soil microorganisms in relation to the fate of Cd in soil remains unclear. In the current study, we investigated the mechanisms underlying the change in mobility of Cd in exogenous Cd-contaminated soil with nZVI and gamma radiation treatments...

## Comparison of the effects of three fungicides on clubroot disease of tumorous stem mustard and soil bacterial community

**Authors:** Liao JJ, Luo LY, Zhang L and more...

**Source:** Journal of Soils and Sediments, 2021, DOI 10.1007/s11368-021-03073-z

**Abstract:** Purpose The application of fungicides is one of the main strategies to prevent clubroot disease. Currently, numerous studies focus on changes in the soil microbial community at different levels of clubroot disease severity. However, the effects of fungicides on the soil microbial community and causative pathogen, Plasmodiophora brassicae, while preventing clubroot disease remain unclear...

## Effect of round-shaped silver nanoparticles on the genetic and functional diversity of soil microbial community in soil and "soil-plant" systems

**Authors:** Macurkova A., Maryska L., Jindrichova B and more...

**Source:** Applied Soil Ecology 168, 2021, DOI 10.1016/j.apsoil.2021.104165

**Abstract:** Silver and silver nanoparticles (AgNPs) are extensively used as antimicrobial agents in various products and consequently enter the soil ecosystem, in which they accumulate and can have adverse effects on above and belowground organisms. Since the composition of the soil microbiome could have a significant impact on soil fertility, we focused, in this study, on the effect of AgNPs on soil microbial communities...

## Soil microbial community responses to the application of a combined amendment in a historical zinc smelting area

**Authors:** Tan CJ, Luo YF, Fu TL

**Source:** Environmental Science and Pollution Research, 2021, DOI 10.1007/s11356-021-16631-2

**Abstract:** Farmland soils that surround a historical zinc smelting area in northwestern Guizhou, China, are characterized by high levels of heavy metal accumulation. Previous studies have mainly focused on the potential risk evaluations of heavy metals in soil and crops. However, at present, the effects of amendment applications on the bioavailability of heavy metals and on microbial community in the heavily contaminated soils of the mining region are still unclear...

## Application, release, ecotoxicological assessment of biocide in building materials and its soil microbial response

**Authors:** Reiss F, Kiefer N, Noll M, Kalkhof S

**Source:** Ecotoxicology and Environmental Safety 224, 2021, DOI 10.1016/j.ecoenv.2021.112707

**Abstract:** Biocides are used in building materials to protect the building against microbial colonization and biodeterioration. However, these biocides are introduced by gradual leaching into soils in proximity of the buildings. This review
discusses the aspects and characteristics of biocides from building materials...

## Effects of Atrazine on Chernozem Microbial Communities Evaluated by Traditional Detection and Modern Sequencing Technology

**Authors:** Yang FS, Gao MY, Lu HG and more...

**Source:** Microorganisms 9(9): 1832, 2021, DOI 10.3390/microorganisms9091832

**Abstract:** Atrazine is a long residual herbicide commonly used in maize fields. Although atrazine can effectively control weeds and improve crop yield, long-term application leads to continuous pollution in the agricultural ecological environment, especially in the soil ecosystem, and its impact on soil microorganisms is still not clear. Four methods were used in the experiment to clarify the effect of atrazine on the bacterial populations of cultivated soil layers of chernozem in a cold region in different periods: high-performance liquid chromatography (HPLC), colorimetry, microplate, and high-throughput sequencing...

## Microbial biomass and activity dynamics in restored lands in a metal contaminated region

**Authors:** McKergow M, Narendrula-Kotha R, Beckett P, Nkongolo KK

**Source:** Ecotoxicology, 2021, DOI 10.1007/s10646-021-02464-9

**Abstract:** Soil microbial communities are important for biogeochemical processes, along with the cycling of nutrients in an ecosystem. Their enzymatic activities are key indicators of their responses to stress. The objective of this research was to assess the effect of land reclamation on microbial biomass and activities in soils impacted by metal contamination...

## Repeated exposure to fungicide tebuconazole alters the degradation characteristics, soil microbial community and functional profiles

**Authors:** Han LX, Kong XB, Xu M, Nie JY

**Source:** Environmental Pollution 287, 2021, DOI 10.1016/j.envpol.2021.117660

**Abstract:** Tebuconazole is a broad-spectrum triazole fungicide that has been extensively applied in agriculture, but its toxicity on soil ecology remains unknown after repeated introduction to soil. This study investigated the degradation of tebuconazole and the changes in soil microbial community composition and functional diversity as well as network complexity in soil repeatedly treated with tebuconazole ...

## Perspectives on microalgae as model organisms toward the standardization of soil algal toxicity test methods

**Authors:** Nam SH, An YJ

**Source:** Comparative Biochemistry and Physiology C-Toxicology and Pharmacology 249, 2021, DOI 10.1016/j.cbpc.2021.109144

**Abstract:** When considering test species for soil ecotoxicity, the development of new model organisms is often suggested to increase the reliability of ecological risk assessments. Ubiquitous soil algae could offer potential test species for assessing various soil pollution levels. Currently, there are few reviews offering comprehensive perspectives on stressors-based toxicological studies using microalgae in soil media, with the majority of scholarly attention paid to the toxicological effects of freshwater algae or marine algae in aquatic ecosystems. In this review, we focus on current toxicological studies of microalgae assessed in soil-related media and suggest considerations for using microalgae in soil toxicity tests...
## Interactions between pyrene and heavy metals and their fates in a soil-maize (Zea mays L.) system: Perspectives from the root physiological functions and rhizosphere microbial community

**Authors:** Wang YH, Li MJ, Liu ZW and more...

**Source:** Environmental Pollution 287, 2021, DOI 10.1016/j.envpol.2021.117616P

**Abstract:** The co-occurrence of polycyclic aromatic hydrocarbons (PAHs) and heavy metals in agricultural soils has become a worldwide food crop security concern. Pot experiments, rhizosphere microbial metagenomic sequencing, and root metatranscriptomic sequencing were performed to investigate the interactions among pyrene, Cu, and Cd in a soil-maize (Zea mays L.) system...

## Responses of microbial community and soil enzyme to heavy metal passivators in cadmium contaminated paddy soils: An in situ field experiment

**Authors:** Jiang YX, Hu T, Peng OU and more...

**Source:** International Biodeterioration & Biodegradation 164, 2021, DOI 10.1016/j.ibiod.2021.105292

**Abstract:** Heavy metal passivators could reduce the transfer of heavy metal ions from soil to crops and exhibit great potential in safe cropping in heavy metal contaminated agricultural soils. However, the effects of heavy metal passivators on microbial diversity and microorganisms-based biogeochemical processes in agricultural soil are poorly explored. In this study, the effects of three heavy metal passivators, viz. lime, silicon (Si) fertilizer, and gypsum, on the structure and function of microorganisms in cadmium (Cd) contaminated paddy soils were investigated...

## Effects of Heavy Metals/Metalloids and Soil Properties on Microbial Communities in Farmland in the Vicinity of a Metals Smelter

**Authors:** Hu XW, Wang JL, Lv Y and more...

**Source:** Frontiers in Microbiology 12, 2021, DOI 10.3389/fmicb.2021.707786

**Abstract:** Microorganisms play a fundamental role in biogeochemical cycling and are highly sensitive to environmental factors, including the physiochemical properties of the soils and the concentrations of heavy metals/metalloids. In this study, high-throughput sequencing of the 16S rRNA gene was used to study the microbial communities of farmland soils in farmland in the vicinity of a lead-zinc smelter...

## Response of Soil Microbial Communities to Different Doses of Glyphosate and Sulfosulfuron in a Calcareous Soil

**Authors:** Mollaee M, Ghadiri H, Zarei M and more...

**Source:** Journal of Agricultural Science and Technology 23(5):1149-1162

**Abstract:** To investigate the response of soil microbial populations to different doses of glyphosate and sulfosulfuron, a factorial experiment based on a complete block design was conducted at Shiraz University, Iran. The factors included different herbicides and dose rates (glyphosate at 0, 540, 1,080, and 4,320 g ae ha(-1) and sulfosulfuron at 0, 12.5, 25, and 50 g ai ha(-1)), and time of measurements (4, 15, 45, and 65 days after herbicides spray). Microbial respiration, microbial biomass carbon, metabolic quotient, dehydrogenase activity, and aerobic heterotrophic bacteria were measured in soil samples...
Heavy metal pollution increases soil microbial carbon limitation: Evidence from ecological enzyme stoichiometry

**Authors:** Xu MZ, Cui YX, Beiyuan JZ and more...

**Source:** SOIL ECOLOGY LETTERS 3(3): 230-241, 2021, DOI 10.1007/s42832-021-0094-2

**Abstract:** Heavy metals can exist in soil for a long time and seriously affect soil quality. The coexistence of various heavy metal pollutants leads to biotoxicity and alters the activity of microorganisms. Soil microbial metabolism plays an important role in nutrient cycling and biochemical processes of soil ecosystem. However, the effects of heavy metal contamination on microbial metabolism in soil are still unclear. This study aims to reveal the responses of microbial metabolic limitation to heavy metals using extracellular enzyme stoichiometry...

The Variations of Bacterial Community Structures in Tailing Soils Suffering from Heavy Metal Contaminations

**Authors:** Gao TP, Li HJ, He YQ and more...

**Source:** Water Air and Soil Pollution 232, 9, 2021, DOI 10.1007/s11270-021-05338-2

**Abstract:** Investigations of the impact of heavy metals on microbial community structure are crucial for bioremediation of the contaminated sites. To this end, high-throughput 16S rRNA sequencing was performed to assess the variations of bacterial communities in 6 heavy metal-contaminated soils sampled from Liujiaping (LJP) and Shanping (SP) lead-zinc tailings situated in northwestern China...

Discovery and characterization of UipA, a uranium- and iron-binding PepSY protein involved in uranium tolerance by soil bacteria

**Authors:** Gallois N, Alpha-Bazin B, Bremond N, and more...

**Source:** ISME Journal 2021, DOI 10.1038/s41396-021-01113-7

**Abstract:** Uranium is a naturally occurring radionuclide. Its redistribution, primarily due to human activities, can have adverse effects on human and non-human biota, which poses environmental concerns. The molecular mechanisms of uranium tolerance and the cellular response induced by uranium exposure in bacteria are not yet fully understood. Here, we carried out a comparative analysis of four actinobacterial strains isolated from metal and radionuclide-rich soils that display contrasted uranium tolerance phenotypes...

Response of soil enzyme activity and bacterial community to copper hydroxide nanofertilizer and its ionic analogue under single versus repeated applications

**Authors:** Tang Q, Xu ZL, Hong AM and more...

**Source:** Science of the Total Environement 796, 2021, DOI 10.1016/j.scitotenv.2021.148974

**Abstract:** Nanosized agrochemicals like nanofertilizers are being applied to soils. Adverse impacts of nanofertilizers on soil microflora were reported in past studies, but only considering a single application. Repeated applications are however more likely to occur in agriculture. We investigated effects of single versus repeated applications of a copper hydroxide nanofertilizer formulation (NFF) on soil enzyme activity and bacterial community...
## Comprehensive characterization of stress tolerant bacteria with plant growth-promoting potential isolated from glyphosate-treated environment

**Authors:** Zhumakayev AR, Voros M, Szekeres A and more...

**Source:** World Journal of Microbiology and Biotechnology 37, 6, 2021, DOI 10.1007/s11274-021-03065-8

**Abstract:** The application of plant growth-promoting bacteria in agricultural systems is an efficient and environment-friendly strategy to improve crop yields and maintain soil quality. However, as different soils have diverse and specific ecological characteristics and may represent adverse abiotic conditions, in vivo application requires the careful selection of the desired beneficial microorganisms. In this study we report Ensifer adhaerens SZMC 25856 and Pseudomonas resinovorans SZMC 25875 isolates recovered from glyphosate-treated soil to possess yet undiscovered plant growth-enhancing potential...

## Evaluating the metabolic functional profiles of the microbial community and alfalfa (Medicago sativa) traits affected by the presence of carbon nanotubes and antimony in drained and waterlogged sediments

**Authors:** Cao WC, Zhu RL, Gong JL and more...

**Source:** Journal of Hazardous Materials 420, 2021, DOI 10.1016/j.jhazmat.2021.126593

**Abstract:** Antimony (Sb) is the ubiquitous re-emerging contaminant greatly accumulated in sediments which has been revealed risky to ecological environment. However, the impacts of Sb (III/V) on microbes and plants in sediments, under different water management with presence of engineering materials are poorly understood.

This study conducted sequential incubation of sediments (flooding, draining and planting) with presence of multiwall carbon nanotubes (MWCNTs) and Sb to explore the influence on microbial functional diversity, Sb accumulation and alfalfa traits...

## Vertical distribution and effect of historical residual organochlorine pesticides on microbial community structure in sediment cores from an abandoned oxidation pond after dredging for 15 years

**Authors:** Wang YF, Liu T, Tang JC and more...

**Source:** Environmental Science and Pollution Research 2021, DOI 10.1007/s11356-021-16192-4

**Abstract:** The vertical distribution pattern of 19 organochlorine pesticides (OCPs), together with microbial ester-linked fatty acid methyl ester (EL-FAME) profiles were investigated in sediments from an abandoned oxidation pond of Ya-Er lake, China, which had been heavily polluted by hexachlorocyclohexanes (HCHs) and chlorobenzenes in 1980s...

## When nanoparticle and microbes meet: The effect of multi-walled carbon nanotubes on microbial community and nutrient cycling in hyperaccumulator system

**Authors:** Chen XF, Wang JC, YouYM and more...

**Source:** Journal of Hazardous Materials 423-A, 2022, DOI 10.1016/j.jhazmat.2021.126947

**Abstract:** Carbon nanotubes can potentially stimulate phytoremediation of heavy metal contaminated soil by promoting plant biomass and root growth. Yet, the regulating mechanism of carbon nanotubes on the rhizosphere micro-
environment and their potential ecological risks remain poorly characterized. The purpose of this study was to systematically evaluate the effects of multi-walled carbon nanotubes (MCNT) on the diversity and structure of rhizosphere soil bacterial and fungal communities, as well as soil enzyme activities and nutrients, in Solanum nigrum L. (S. nigrum)-soil system...

## Ecological network analysis reveals distinctive microbial modules associated with heavy metal contamination of abandoned mine soils in Korea

**Authors:** Chun SJ, Kim YJ, Y Nam KH

**Source:** Environmental Pollution 289, 2021, DOI 10.1016/j.envpol.2021.117851

**Abstract:** Heavy metal pollution in soil around abandoned mine sites is one of the most critical environmental issues worldwide. Soil microbes form complex communities and perform ecological functions individually or in cooperation with other organisms to adapt to harsh environments. In this study, we investigated the distribution patterns of bacterial and fungal communities in non-contaminated and heavy metal-contaminated soil of the abandoned Samkwang mine in Korea to explore microbial interaction mechanisms and their modular structures...

## Ant nests as a microbial hot spots in a long-term heavy metal-contaminated soils

**Authors:** Klimek B, Poliwka-Modliborek H, Grzes IM

**Source:** Environmental Science and Pollution Research 2021, DOI 10.1007/s11356-021-16384-y

**Abstract:** Interactions between soil fauna and soil microorganisms are not fully recognized, especially in extreme environments, such as long-term metal-polluted soils. The purpose of the study was to assess how the presence of Lasius niger ants affected soil microbial characteristics

in a long-term metal-polluted area (Upper Silesia in Poland)...

## Microbial diversity alteration reveals biomarkers of contamination in soil-river-lake continuum

**Authors:** Bourhane Z, Lanzen A, Cagnon C and more...

**Source:** Journal of Hazardous Material 421, 2022, DOI 10.1016/j.jhazmat.2021.126789P

**Abstract:** Microbial communities inhabiting soil-water-sediment continuum in coastal areas provide important ecosystem services. Their adaptation in response to environmental stressors, particularly mitigating the impact of pollutants discharged from human activities, has been considered for the development of microbial biomonitoring tools, but their use is still in the infancy. Here, chemical and molecular (16S rRNA gene metabarcoding) approaches were combined in order to determine the impact of pollutants on microbial assemblages inhabiting the aquatic network of a soil-water-sediment continuum around the Ichkeul Lake (Tunisia), an area highly impacted by human activities...

## Changes in the Composition of the Soil Bacterial Community in Heavy Metal-Contaminated Farmland

**Authors:** Tseng SC, Liang CM, Chia T, Ton SS

**Source:** International Journal of Environmental Research and Public Health 18, 16, 2021, DOI 10.3390/ijerph18168661

**Abstract:** The structural changes of microorganisms in soil are the focus of soil indicators research. The purpose of this study was to investigate the changes in the composition of the soil bacterial community in heavy metal-contaminated soil. A total of six soil samples (two sampling times) were collected from contaminated farmland at three different depths (surface, middle, and deep layer)...
# Cadmium retention and microbial response in volcanic soils along gradients of soil age and climate on the Galapagos Islands

**Authors:** Rechberger MV, Roberti D, Phillips A and more...

**Source:** Journal of Environmental Quality 2021, DOI 10.1002/jeq2.20275

**Abstract:** The behavior of trace metals may vary strongly in the course of volcanic soil development. Cadmium retention in soils is specifically important for some Galapagos islands where agriculture is leading to anthropogenic Cd contamination. To assess the influence of soil development factors on soil Cd retention and toxicity, we performed Cd sorption-desorption experiments with volcanic topsoils from the Galapagos Islands sampled along gradients of (a) substrate age (chronosequence, 1.5-1,070 ka) and (b) climate (elevation sequence, 47-866 m asl) ranging from arid lowland areas to humid highland areas...

# Microbial Community's Dynamic Response to Fomesafen Usage in Chernozems of Northeast China

**Authors:** Yang FS, Wang YB, Huang YA, Wei YN and more...

**Source:** Diversity Basel 13, 8, 2021, DOI 10.3390/d13080340

**Abstract:** The main purpose of this study was to explore the effects of the recommended usage level and twice the recommended usage level of the long-acting herbicide fomesafen on the soil enzymes and microbial community structure in chernozems of soybean fields. Culturable microbial biomass and phospholipid fatty acids (PLFA) were used as the main references for this evaluation...

# Effect of an Equal Dose of Polymetallic Pollution on the Microbiological Characteristics of Two Soils with Different Organic Carbon Contents

**Authors:** Terekhova VA, Fedoseeva EV, Kiryushina AP and more...

**Source:** Water Air and Soil Pollution 232, 2021, DOI 10.1007/s11270-021-05174-4

**Abstract:** Reliable stable indicators are very important for assessing soil quality. This paper compares the dynamics of microbial parameters in two different soils (rich/poor in organic carbon), contaminated in laboratory experiments with an equal dose of heavy metals (HMs) after 30 and 90 days. For this purpose, the changes in the number, biomass, and taxonomic structure of bacterial and fungal communities were assessed in microcosm experiments using an organic carbon-rich ordinary chernozem (Ch-humus-rich soil) and a depleted carbon agrozem (Ag-humus-poor soil), spiked with high concentrations of a zinc, lead, and copper solution (1100 Zn+660 Cu+650 Pb mg per kg soil) ...

# Dinotefuran alters Collembola-fungi-bacteria interactions that control mineralization of maize and soil organic carbon

**Authors:** Yu ZY, Schmidt O, Zhao Y and more...

**Source:** Journal of Hazardous Materials 18, 2021, DOI 10.1016/j.jhazmat.2021.126391

**Abstract:** Rare studies investigated influence of neonicotinoid insecticides on the whole soil biota including non-target invertebrates and microorganisms. And less is known about the consequent intervention on soil C processes. This study aimed to decipher Collembola-fungi-bacteria interactive effects on pathways of maize C translocation, combining isotopic tracer analysis of relevant compartments with high-throughput sequencing for bacterial and fungal genetic profiles...
Microbiological Nitrogen
Transformations in Soil
Treated with Pesticides and
Their Impact on Soil
Greenhouse Gas Emissions

**Authors:** Jezierska-Tys S, Joniec J, Bednarz J, Kwiatkowska E

**Source:** Agriculture Basel 11, 8, 2021, DOI 10.3390/agriculture11080787

**Abstract:** Research was conducted in connection with the pressure exerted by man on the environment through the use of pesticides. The aim of the study was to assess the impact of pesticides on soil and to evaluate the effect of these changes on greenhouse gas emissions into the atmosphere. The research was carried out on soil sown with oilseed rape...

Effects of heavy metals and
organic matter fractions on
the fungal communities in
mangrove sediments from
Techeng Isle, South China

**Authors:** Xiao YZ, He MY, Xie JF and more...

**Source:** Ecotoxicology and Environmental Safety 222, 2021, DOI 10.1016/j.ecoenv.2021.112545

**Abstract:** Heavy metal pollution has become a serious environmental problem in mangrove ecosystems and has attracted more attention. Most of previous studies have mainly focused on the effects of heavy metals on bacterial communities in mangrove sediments. This study was the first to investigate the effects of heavy metals (e.g., As, Co, Cr, Cu, Mn, Ni, Pb, V and Zn) and organic matter fractions (including total organic carbon (TOC), total nitrogen (TN), and total sulfur (TS)) on the fungal communities in mangrove sediments from Techeng Isle, South China...

ERA / PUBLICATIONS

SCIENTIFIQUES /

MICROBIOLOGIE ET
CONTAMINANTS /

Antibiotiques et
antibiorésistances

Incubation trial indicated the
earthworm intestinal bacteria
as promising biodigestor for
mitigating tetracycline
resistance risk in
anthropogenic disturbed
forest soil

**Authors:** Chao HZ, Zheng XX, Xia R and more...

**Source:** Science of the Total Environement 798, 2021, DOI 10.1016/j.scitotenv.2021.149337

**Abstract:** The continuous input of antibiotics due to frequent anthropogenic activities have increased the dissemination risk of antibiotic resistance genes (ARGs) in forest soil. As soil engineers, it remains undear whether earthworm intestinal microbial communities might play a role in controlling the ARG proliferation in forest soil. This study collected forest soil in the Yangtze River Delta. China, and its resident Metaphire guillelmi to investigate the interaction between tetracycline (50 mu g kg (-1)) and the bacteria in worm gut and soil...

Sulfamethoxazole
biodegradation and impacts
on soil microbial communities
in a Bolivian arid high altitude
catchment

**Authors:** Archundia D, Martins JMF, Lehembre F and more...

**Source:** Chemosphere 284, 2021, DOI 10.1016/j.chemosphere.2021.131335
**Abstract:** The processes controlling antibiotics fate in ecosystems are poorly understood, yet their presence can inhibit bacterial growth and induce the development of bacterial resistance. Sulfamethoxazole (SMX) is one of the most frequently detected sulfonamides in natural environments due to its low metabolism and molecular properties. This work presents pioneering results on SMX biodegradation and impact in high altitude soils (Bolivian Alti-plano), allowing a better understanding of the persistence, spread and impact of this antibiotic at the global watershed scale...

## Fertilization with poultry litter increases the abundance of antibiotic-resistant bacteria in tropical soil: a microcosm study

**Authors:** Chaves-Ulate C, Granados-Chinchilla F, Rodriguez C

**Source:** Water Air and Soirl Pollution 232, 10, 2021, DOI 10.1007/s11270-021-05347-1

**Abstract:** Various antimicrobial agents are used in the poultry industry to treat microbial infections and prevent disease or as growth promoters. As a result, poultry litter (PL) can contain antibiotic residues (AR), antibiotic-resistant bacteria (ARB), and antibiotic resistance genes. Still, PL is used in many countries as a fertilizer and feed supplement for cattle. To evaluate whether usage of PL in agriculture leads to the accumulation of AR and ARB accumulate in the soil, we (i) measured the concentration of monensin, tylosin, ciprofloxacin, oxytetracycline, and chlortetracycline and the abundance of culturable monensin-, tylosin-, and ciprofloxacin-resistant bacteria in 15 commercial PL samples and (ii) exposed soil microcosms...

Antibiotic resistance, antimicrobial residues, and bacterial community diversity in pasture-raised poultry, swine, and beef cattle manures

**Authors:** Rothrock MJ, Min BR, Castleberry L and more...

**Source:** Journal of Animal Science 99, 8, 2021, DOI 10.1093/jas/ska144

**Abstract:** Animal manure can be a source of antibiotic-resistant genes (ARGs) and pharmaceutical residues; however, few studies have evaluated the presence of ARG in pasture-raised animal production systems. The objective of this study was to examine changes in microbiome diversity and the presence of antibiotic residues (ABRs) on three farms that contained a diverse range of animal species: pasture-raised poultry (broiler and layer), swine, and beef cattle...

## Impact of chicken litter pre-application treatment on the abundance, field persistence, and transfer of antibiotic resistant bacteria and antibiotic resistance genes to vegetables

**Authors:** Subirats J, Murray R, Yin XL and more...

**Source:** Science of the Total Environment 801, 2021, DOI 10.1016/j.scitotenv.2021.149718

**Abstract:** Treatment of manures prior to land application can potentially reduce the abundance of antibiotic resistance genes and thus the risk of contaminating crops or water resources. In this study, raw and composted chicken litter were applied to field plots that were cropped to carrots, lettuce and radishes. Vegetables were washed per normal culinary practice before downstream analysis. The impact of composting on manure microbial composition, persistence of antibiotic resistant bacteria in soil following application, and distribution of antibiotic resistance genes and bacteria on washed vegetables were determined...
## Assessing Antibiotics Biodegradation and Effects at Sub-inhibitory Concentrations by Quantitative Microbial Community Deconvolution

**Authors:** Duygan BDO, Gaille C, Fenner K, van der Meer JR

**Source:** Frontiers in Environmental Science 9, 2021, DOI 10.3389/fenvs.2021.737247

**Abstract:** Antibiotics in the environment cause widespread concern as a result of their potent inhibitory action on microbial growth and their role in potentially creating selective conditions for proliferation of antibiotic resistant bacteria. Comprising a carbon skeleton, antibiotics should be amenable to microbial biodegradation, but this is still largely uncharted territory because of their simultaneous strong toxicity. In this study, we estimated potential antibiotics degradation by and effects on mixed microbial communities at concentrations sufficiently high to allow sensitive detection of biomass growth, but simultaneously, low enough to mitigate their toxic action...

## Manure microbial communities and resistance profiles reconfigure after transition to manure pits and differ from those in fertilized field soil

**Authors:** Sukhum KV, Vargas RC, Boolchandani M and more...

**Source:** MBIO 12, 3, 2021, DOI 10.1128/mBio.00798-21

**Abstract:** In agricultural settings, microbes and antimicrobial resistance genes (ARGs) have the potential to be transferred across diverse environments and ecosystems. The consequences of these microbial transfers are unclear and understudied. On dairy farms, the storage of cow manure in manure pits and subsequent application to field soil as a fertilizer may facilitate the spread of the mammalian gut microbiome and its associated ARGs to the environment...

## Insights into the role of the fungal community in variations of the antibiotic resistome in the soil collembolan gut microbiome

**Authors:** Wang YF, Qiao M, Duan GL and more...

**Source:** Environmental Science & Technology 55, 17: 11784-11794, 2021, DOI 10.1021/acs.est.0c08752

**Abstract:** Fertilization is known to affect antibiotic-resistance gene (ARG) patterns in the soil, even in the gut of soil fauna. Here, we conducted a microcosm experiment to investigate differences of effects of different fertilizers on collembolan gut ARG profiles and to further explore the microecological mechanisms that cause the differences...

## From the soil to the clinic: the impact of microbial secondary metabolites on antibiotic tolerance and resistance

**Authors:** Perry EK, Meirelles LA, Newman DK

**Source:** Nature Reviews Microbiology 2021, DOI 10.1038/s41579-021-00620-w

**Abstract:** Secondary metabolites profoundly affect microbial physiology, metabolism and stress responses. Increasing evidence suggests that these molecules can modulate microbial susceptibility to commonly used antibiotics; however, secondary metabolites are typically excluded from standard antimicrobial susceptibility assays. This may in part account for why infections by diverse opportunistic bacteria that produce secondary metabolites often exhibit discrepancies between clinical antimicrobial susceptibility testing results and clinical treatment outcomes...
Distribution and Influence on
the Microbial Ecological
Relationship of Antibiotic
Resistance Genes in Soil at a
Watershed Scale

Authors: Hao YL, Li G, Xiao ZF and more...

Source: Sustainability 13, 17, 2021, DOI 10.3390/su13179748

**Abstract:** Antibiotic resistance genes (ARGs) are ubiquitous in the environment, with previous studies mainly focusing on the terrestrial ecosystem, which is prone to higher antibiotic application. However, the characteristics, distribution pattern, and driving factors of soil ARGs at the macro scale are still unclear. In this study, the soil ARGs, antibiotics, mobile genetic elements (MGEs), soil properties, toxic metals, polycyclic aromatic hydrocarbons (PAHs), and bacterial community in the Taipu River Basin were analyzed to investigate the distribution and dissemination of ARGs at a watershed scale...

Persistence and spread of
tetracycline resistance genes
and microbial community
variations in the soil of animal
corrals in a semi-arid planted
forest

Authors: Nejidat A, Diaz-Reck D, Gelfand I, Zaady E

Source: FEMS Microbiology Ecology 97, 8, 2021, DOI 10.1093/femsec/fiab106

**Abstract:** At the spring, goat and sheep herds are transferred to planted forests, in a semi-arid region in the northern Negev Desert, Israel, to reduce herbaceous biomass and, fire risk. The herds are held overnight in corrals for about 4 months, enriching the soil with organic matter and nitrogen. This research examined the effect of these enrichments on soil bacterial community structure (BCS) and the abundance of tetracycline resistance genes (TRGs) in active and abandoned corrals (1-10-years-old)...

Post-digestate composting
shifts microbial composition
and degrades antimicrobial
resistance genes

Authors: Gurmessa B, Milanovic V, Pedretti EF and more...

Source: Bioresource Technology 340, 2021, DOI 10.1016/j.biortech.2021.125662

**Abstract:** Post-digestate treatments may reduce the risk linked to Antibiotic Resistant Genes (ARGs) release with digestate direct land application. Thus, this study aimed to evaluate post-digestate composting and co-composting with biogas production feedstock (maize silage, food processing waste, and poultry litter) effect on abundance of selected ARGs: *erm(B)*, *tet(K)*, *tet(M)*, *tet(O)*, and *tet(S)* genes...

Insights into the mechanism
of the interference of
sulfadiazine on soil microbial
community and function

Authors: Qiu LL, Daniell TJ, Banwart SA and more...

Source: Journal of Hazardous Material 419, 2021, DOI 10.1016/j.jhazmat.2021.126388

**Abstract:** The accumulation of sulfonamides in the soil environment possessed the potential to change soil microbial community and function. Metabolomics is capable of providing insights into the carbon metabolic pool and molecular mechanisms associated with external stressors. Here we evaluated alternations in soil bacterial community and soil metabolites profiles under sulfadiazine (SDZ) exposure and proposed a potential mechanism that SDZ accumulation in soil affected soil organic matter (SOM) cycling...
# The Effect of Clarithromycin Toxicity on the Growth of Bacterial Communities in Agricultural Soils

**Authors:** Rodriguez-Gonzalez L, Santas-Miguel V, Campillo-Cora C and more...

**Source:** Processes 9, 8, 2021, DOI 10.3390/pr9081303

**Abstract:** The presence of antibiotics in different environmental matrices is a growing concern. The introduction of antibiotics into the soil is mainly due to sewage treatment plants. Once in the soil, antibiotics may become toxic to microbial communities and, as a consequence, can pose a risk to the environment and human health. This study evaluates the potential toxicity of the antibiotic clarithromycin (CLA) in relation to the bacterial community of 12 soils with different characteristics...

# Enzymatic activity and microbial diversity of sod-podzolic soil microbiota using 16s rrna amplicon sequencing following antibiotic exposure

**Authors:** Trifonova T, Kosmacheva A, Sprygin A, Chesnokova S, Byadovskaya O

**Source:** Antibiotic Basel 10, 8, 2021, DOI 10.3390/antibiotics10080970

**Abstract:** Antibiotic contamination of the environment negatively affects soil fertility by disrupting natural microbial communities. Currently, the study of the effect of antibacterial drugs on soils typical in Russia, which are of great importance for agriculture, is insufficient. Despite a rapid increase in the number of metagenomic studies, this article is the first publication devoted to the microbial diversity of sod-podzolic soil and its relationship with enzymatic activity ...

# Antibiotic Resistance Gene-Carrying Plasmid Spreads into the Plant Endophytic Bacteria using Soil Bacteria as Carriers

**Authors:** Xu H, Chen ZY, Huang RY and more...

**Source:** Environmental Science & Technology 55: 10462-10470, 2021, DOI 10.1021/acs.est.1c01615

**Abstract:** Applications of animal manure and treated wastewater could enrich antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs) in the plant microbiome. However, the mechanistic studies of the transmission of ARB and ARGs from the environment to plant endophytic bacteria were few. Herein, a genetically engineered fluorescent Escherichia coli harboring a conjugative RP4 plasmid that carries three ARGs was used to trace its spread into *Arabidopsis thaliana* interior in a tetracycline-amended hydroponic system in the absence or presence of a simulated soil bacterial community...

ERA / PUBLICATIONS
SCIENTIFIQUES /
MICROBIOLOGIE ET
CONTAMINANTS /
Biocontrôle

# Using crop diversity to lower pesticide use: Socio-ecological approaches

**Authors:** Thomine E, Mumford J, Rusch A, Desneux N

**Source:** SCIENCE OF THE TOTAL ENVIRONMENT 804: 150156, 2022, DOI 10.1016/j.scitotenv.2021.150156

**Abstract:** The farming practices adopted since the end of the Second World War, based on large areas of monocultures and chemical use, have adversely affected the health of farmers and consumers and dramatically reduced farmland biodiversity. As a consequence, many studies over more than twenty years have stated that agriculture is facing three main challenges: (1) feeding the growing world population (2) with more environmentally friendly products (3) at a reasonable return for the producer. Increasing the efficacy of biocontrol could be one lever for agriculture to meet these expectations...
## Impacts of decaying aromatic plants on the soil microbial community and on tomato seedling growth and metabolism: suppression or stimulation?

**Authors:** Ainalidou A, Bouzoukla F, Menkissoglu-Spiroudi U and more...

**Source:** Plant Basel 10, 9, 2021, DOI 10.3390/plants10091848

**Abstract:** This study provides insight into changes in the features of tomato seedlings growing in soils enriched with spearmint, peppermint, or rosemary leaves and into changes in the microbial communities of these soils used as seedbeds; an organic amendment was also applied as a positive control. While the soil microbial community flourished in the presence of all three aromatic plants, tomato growth was inhibited or stimulated depending on the plant that was used...

## Benefits of corn-cob biochar to the microbial and enzymatic activity of soybean plants grown in soils contaminated with heavy metals

**Authors:** Haddad SA, Lemanowicz J

**Source:** Energies 14, 18, 2021, DOI 10.3390/en14185763

**Abstract:** Synchronous effects of biochar on heavy metals stress, microbial activity and nodulation process in the soil are rarely addressed. This work studied the effects, under greenhouse conditions, of selected heavy metals Cd2+, Pb2+ and Ni2+ on soybean plants grown in two different soils amended with biochar, and studied their effect on the microbial and enzymatic activity...

## Biopesticide evaluation from lab to greenhouse scale of

### essential oils used against macrosiphum euphorbiae

**Authors:** Dunan L, Malanga T, Bearez P, Benhamou S et al.

**Source:** AGRICULTURE-BASEL 11(9): 867, 2021, DOI 10.3390/agriculture11090867

**Abstract:** Aphids are recognized as a major threat to economically important crops. Their control is predominantly based on synthetic insecticides that are detrimental to human health and the environment. Botanical pesticides based on essential oils (EOs) are a promising alternative. In this study, the entomotoxicity of green anise and fennel EO fumigation was tested on the potato aphid Macrosiphum euphorbiae. Three different settings of increasing scale were considered (leafletlet, whole plant and greenhouse) to appraise the consistency of EO impact from controlled laboratory to greenhouse production conditions...

## Apiaceae essential oils and their constituents as insecticides against mosquitoes-A review

**Authors:** Spinozzi E, Maggi F, Bonacucina G, Pavela et al.

**Source:** INDUSTRIAL CROPS AND PRODUCTS 171:113892, 2021, DOI 10.1016/j.indcrop.2021.113892

**Abstract:** Apiaceae is a family encompassing medicinal and aromatic plants. They produce essential oils (EOs) inside oil canals, known as ducts and vittae, which occur in their vegetative and reproductive organs. Given the high EO yields and the availability of raw material from cultivations widespread all over the world, Apiaceae are exploitable for different industrial applications. An interesting perspective is their utilization in the fabrication of botanical insecticides effective against insect vectors of public importance such as mosquitoes, aiming to be ecofriendly alternatives to synthetic insecticides. On this basis, in the current review, we collected scientific evidence supporting the use of Apiaceae EOs and their major constituents as active ingredients in insecticidal formulations against larvae and adults of several mosquitoes
that are important pathogen vectors (e.g., Anopheles, Aedes and Culex species)...

## Impact of biocontrol microbes on soil microbial diversity in ginger (Zingiber officinale Roscoe)

**Authors:** Huang ZQ, Liu BW, Yin Y, Liang F and more...

**Source:** Pest Management Science 2021, DOI 10.1002/ps.6595

**Abstract:** Bacteria are the most diverse and abundant group of soil organisms that influence plant growth and health. Bacillus and Trichoderma are commonly used as biological control agents (BCA) that directly or indirectly act on soil bacteria. Therefore, it is essential to understand how the applied microbes impact the indigenous microbial community before exploring their activity in the control of soilborne diseases...

ERA / PUBLICATIONS

SCIENTIFIQUES /

MICROBIOLOGIE ET

CONTAMINANTS /

Bioremédiation

## System biology analysis of endosulfan biodegradation in bacteria and its effect in other living systems: modeling and simulation studies

**Authors:** Bhandari G, Sharma M, Negi S and more...

**Source:** Journal of Biomolecular Structures & Dynamics 2021, DOI 10.1080/07391102.2021.1982773

**Abstract:** Endosulfan is a broadly applied cyclodiene insecticide which has been in use across 80 countries since last 5 decades. Owing to its recalcitrant nature, endosulfan residues have been reported from air, water and soil causing toxicity to various non-target organisms. Microbial decontamination of endosulfan has been reported previously by several authors...

## Photo-biodegradation of imidacloprid under blue light-emitting diodes with bacteria and co-metabolic regulation

**Authors:** Elumalai P, Yi XH, Cai TT and more...

**Source:** Environmental Research 201, 2021, DOI 10.1016/j.envres.2021.111541

**Abstract:** Imidacloprid (IMI) is existence in the soil environment with a half-life habitually more than hundred days. This study targets to determine, identify and characterize photo-biodegradation bacteria from neonicotinoids (NEOs) contaminated agricultural field soils. The subsurface soil had a higher level contamination of NEOs...

## A field study reveals links between hyperaccumulating Sedum plants-associated bacterial communities and Cd/Zn uptake and translocation

**Authors:** Wu YJ, Santos SS, Vestergard M and more...

**Source:** Science of the Total Environment 805, 2022, DOI 10.1016/j.scitotenv.2021.150400

**Abstract:** Hyperaccumulating ecotypes of Sedum plants are promising Cd/Zn phytoextractors, with potential for leveraging its rhizospheric or endophytic microbiomes to improve phytoremediation efficiency. However, research of bacteria associated with Sedum at field scale is still lacking. Here, we presented a detailed investigation of the bacterial microbiome of hyperaccumulating Sedum ecotypes (S. alfredii and S. plumbizincicola) and a
nonhyperaccumulating S. *alfredii* ecotype, which
grow at different soil environments...

Functional analysis of
chlorpyrifos biodegradation
in agricultural soils
augmented with a three-
strain bacterial consortium

Authors: Islam N, Iyer R

Source: Water Air and Soil Pollution 232, 10, 2021, DOI 10.1007/s11270-021-05349-z

**Abstract:** Chlorpyrifos (CP) is a widely used organophosphate (OP) insecticide and a potent environmental neurotoxin. This research focuses on the potential of bacteria, both native to agricultural soil and part of a designed consortium composed of laboratory strains, to completely degrade CP and its toxic metabolites...

Endophytic bacterium CIMAP-
A7 mediated amelioration of
atrazine induced phyto-
toxicity in Andrographis
paniculata

Authors: Tripathi P, Yadav R, Das P and more...

Source: Environmental Pollution 287, 2021, DOI 10.1016/j.envpol.2021.117635

**Abstract:** The presence of atrazine, a triazine herbicide, and its residues in agriculture soil poses a serious threat to human health and environment through accumulation in edible plant parts. Hence, the present study focused on atrazine induced stress amelioration of Andrographis paniculata, an important medicinal plant, by a plant growth promoting and atrazine degrading endophytic bacterium CIMAP-A7 inoculation...

Assessing the arsenic-
saturated biochar recycling
potential of vermitechnology:
Insights on nutrient recovery,

metal benignity, and
microbial activity

Authors: Roy S, Sarkar D, Datta R and more...

Source: Chemosphere 286, 1, 2021, DOI 10.1016/j.chemosphere.2021.131660

**Abstract:** Biochar mediated pollutant removal is gaining attention because of high efficiency of the process. However, effective recycling avenues of the pollutant-saturated biochars are scarce in the knowledge base; while such materials can be a new source of long-range contamination...

Disentangling the role of
ectomycorrhizal fungi in plant
nutrient acquisition along a
Zn gradient using X-ray
imaging

Authors: Zhang KL, Tappero R, Ruytinx J and more...

Source: Science of the Total Environment 801, 2021, DOI 10.1016/j.scitotenv.2021.149481

**Abstract:** Zinc (Zn) is a plant essential micronutrient involved in a wide range of cellular processes. Ectomycorrhizal fungi (EMF) are known to play a critical role in regulating plant Zn status. However, how EMF control uptake and trans location of Zn and other nutrients in plant roots under different Zn conditions is not well known. Using X-ray fluo-rescence imaging, we found the EMF species Suillus luteus increased pine root Zn acquisition under low Zn concentrations...

An efficient manganese-
oxidizing fungus
Cladosporium halotolerans
strain XM01: Mn(II)
oxidization and Cd adsorption
behavior

Authors: Wang M, Xu ZX, Dong B and more...

Source: Chemosphere 28, 1, 2022, DOI 10.1016/j.chemosphere.2021.132026
**Abstract:** The applications of biogenic Mn oxides (BMOs) formed by Mn-oxidizing fungus in decontaminating heavy metals have attracted increasing attention. In this study, an efficient Mn-oxidizing fungus was isolated from soil and identified as Cladosporium halotolerans strain XM01. The Mn(II) adsorption and oxidation activities of this strain were investigated, showing significantly high removal and oxidation rates of soluble Mn(II)...

## A biogenic microbial biosurfactin that degrades difenoconazole fungicide with potential antimicrobial and oil displacement properties

**Authors:** Satapute P, Jogaiah S

**Source:** Chemosphere 286, 1, 2022, DOI 10.1016/j.chemosphere.2021.131694

**Abstract:** Surfactin is a bacterial lipopeptide and an influential biosurfactant mainly known for excellent surfactant ability. The amphiphilic nature of surfactin helps it to sustain under hydrophobic and hydrophilic conditions. In this investigation, a bacterium strain (BTKU3) that produces biosurfactant were isolated from oil-contaminated soil. Based on the blue agar plate (Bap) assay, the BTKU3 strain was found to be promising for biosurfactant production...

## Biochar and earthworms synergistically improve soil structure, microbial abundance, activities and pyraclostrobin degradation

**Authors:** Zhang QM, Li SZ, Saleem M and more...

**Source:** Applied Soil Ecology 168, 2021, DOI 10.1016/j.apsoil.2021.104154

**Abstract:** As a widely used fungicide, the toxic impacts of pyraclostrobin on soil ecosystems have attracted extensive concern. Thus, it is very important and meaningful to develop effective strategies to alleviate its toxic effects. Therefore, in this study, we used corn straw-derived biochar

produced at 500 degrees C as a soil amendment to study its effects on soil microbial populations, aggregation, enzymatic activities, and earthworm *Eisenia fetida* in pyraclostrobin-contaminated soil...

## Theoretical design and process control of neonicotinoids insecticides suitable for synergistic degradation with the rubisco enzyme from rhizobia and carbon-fixing bacteria in soil

**Authors:** Deng ZY, Ren ZX, Sun SH, Wang YJ

**Source:** Environmental Sciences and Pollution Research 2021, DOI 10.1007/s11356-021-16531-5

**Abstract:** In this study, we studied and developed the modification schemes of environmentally friendly substitutes of neonicotinoid insecticides (NNIs) along with the regulatory measures that effectively enhanced the synergistic degradation of NNIs by soil rhizobia and carbon-fixing bacteria. Firstly, the binding ability of NNIs to the two key proteins was characterized by molecular docking; secondly, the mean square deviation decision method, which is a comprehensive evaluation method, was used to investigate the binding ability of NNI molecules with the two Rubisco rate-limiting enzymes...

## Enhanced Bioremediation of Heavy Metals from Phosphate Processing Wastewater Using the Indigenous Bacterium *Serratia rubidaea* NCTC12971

**Authors:** Moula A, Ellafi A, Borgi MA and more...

**Source:** Geomicrobiology Journal 2021, DOI 10.1080/01490451.2021.1979696

**Abstract:** Bioremediation using microorganisms have become innovative efficient tools for detoxifying a wide variety of urban and industrial wastewaters. In this study, we investigated for the first time the microbial detoxification of
phosphate processing wastewaters (PPWW) by using the indigenous bacterium Serratia rubidaea NCTC12971, having numerous plant growth promoting traits...

## Bacterial consortium biotransformation of pentachlorophenol contaminated wastewater

**Authors:** Ammeri RiW, Simeone GD, Hassen W, and more...

**Source:** Archives of Microbiology DOI10.1007/s00203-021-02589-9

**Abstract:** The aims of this study were (i) to compare PCP removal (100 mg L-1) by two bacterial consortia B-1 and B-2 in sterile wastewater (STWW) and liquid mineral medium (MSM), (ii) PCP effect in biofilm formation and antimicrobial susceptibility...

## Passivation of multiple heavy metals in lead-zinc tailings facilitated by straw biochar-loaded N-doped carbon aerogel nanoparticles: Mechanisms and microbial community evolution

**Authors:** Li JH, Xia CG, Cheng R and more...

**Source:** Science of the Total Environment 803, 2021, DOI 10.1016/j.scitotenv.2021.149866

**Abstract:** Heavy metal (HM) soil pollution has become an increasingly serious problem with the development of industries. Application of biochar in HMs remediation from contaminated environment has attracted considerable research attention during the past decade. Although the mechanism of HMs passivation with biochar has been investigated, effects and mechanisms of interaction among soil-indigenous microbes and novel carbon matrix composites for HMs adsorption and passivation are still unclear...

## Microbial Community Diversity Dynamics in Acid Mine Drainage and Acid Mine Drainage-Polluted Soils: Implication on Mining Water Irrigation Agricultural Sustainability

**Authors:** Munyai R, Ogola HJO, Modise DM

**Source:** FRONTIERS IN SUSTAINABLE FOOD SYSTEMS 5, 2021, DOI 10.3389/fsufs.2021.701870

**Abstract:** Environmental degradation related to mining-generated acid mine drainage (AMD) is a major global concern, contaminating surface and groundwater sources, including agricultural land. In the last two decades, many developing countries are expanding agricultural productivity in mine-impacted soils to meet food demand for their rapidly growing population. Further, the practice of AMD water (treated or untreated) irrigated agriculture is on the increase, particularly in water-stressed nations around the world. For sustainable agricultural production systems, optimal microbial diversity, and functioning is critical for soil health and plant productivity. Thus, this review presents up-to-date knowledge on the microbial structure and functional dynamics of AMD habitats and AMD-impacted agricultural soils...

## Microbial investigations of new hydrogel-biochar composites as soil amendments for simultaneous nitrogen-use improvement and heavy metal immobilization

**Authors:** Zhang LX, Guan YT

**Source:** Journal of Hazardous Materials 424, A, 2021, DOI 10.1016/j.jhazmat.2021.127154

**Abstract:** Agricultural sustainability is challenging because of increasingly serious and co-existing issues, e.g., poor nitrogen-fertilizer use and heavy metal pollution. Herein, we introduced a new poly (acrylic acid)-grafted chi-tosan and biochar
composite (PAA/CTS/BC) for soil amendment, and provided a first microbial insight into how PAA/CTS/BC amendment simultaneously improved nitrogen cycling and immobilized heavy metals...

## Interactions among heavy metal bioaccessibility, soil properties and microbial community in phyto-remediated soils nearby an abandoned realgar mine

**Authors:** Xiao WW, Lin GB, He XM and more...

**Source:** Chemosphere 286, 1, 2021, DOI 10.1016/j.chemosphere.2021.131638

**Abstract:** Soil samples were collected from a representative arsenic (As) contaminated region under phytoremediation of hyperaccumulation plants. Relative abundance and diversity of microbial communities in the soil samples were characterized via 16S rRNA genes sequencing...

## Soil microbial community and abiotic soil properties influence Zn and Cd hyperaccumulation differently in Arabidopsis halleri

**Authors:** Kushwaha P, Neilson JW, Maier RM, Babst-Kostecka A

**Source:** Science of the Total Environment 803, 2022, DOI 10.1016/j.scitotenv.2021.150006

**Abstract:** Soil contamination with trace metal(loid) elements (TME) is a global concern. This has focused interest on TME tolerant plants, some of which can hyperaccumulate extraordinary amounts of TME into above-ground tissues, for potential treatment of these soils. However, intra-species variability in TME hyperaccumulation is not yet sufficiently understood to fully harness this potential. Particularly, little is known about the rhizosphere microbial communities associated with

hyperaccumulating plants and whether or not they facilitate TME uptake...

## Effects of arbuscular mycorrhizal fungi on frond antimony enrichment, morphology, and proteomics in *Pteris cretica* var. *nervosa* during antimony phytoremediation

**Authors:** Xi L, Shen YaQ, Zhao X and more...

**Source:** Science of the Total Environment 804, 2022, DOI 10.1016/j.scitotenv.2021.149904

**Abstract:** *Pteris cretica* var. *nervosa* is a dominant fern species found in antimony (Sb) mining areas, capable of forming symbiosis with arbuscular mycorrhizal fungi (AMF), especially with those members of the Glomus genus. Despite this fern's relevance and the potential contribution of mycorrhizal symbiosis to phytoremediation, the AMF's im-pact on P. var. *nervosa* phytoremediation of Sb remains unknown. Here, we exposed *P. var. nervosa* to different concentrations of Sb for 6 months...

## Critical review on microbial community during in-situ bioremediation of heavy metals from industrial wastewater

**Authors:** Sharma P, Pandey AK, Kim SH and more...

**Source:** Environmental Technology & innovation 4, 2021, DOI 10.1016/j.eti.2021.101826

**Abstract:** This review aims to summarise recent studies on in-situ microbial remediation of heavy metals from industrial wastewater. The major environmental issue of heavy metal(s) pollution in groundwater is worldwide due to the continuous development of industrial activities. Microbial remediation has been reported to show intense changes in the microbial diversity induced by heavy metals, environmental pollution, and
adaptation mechanisms that enable microbes to live in environments contaminated by metals...

## Unraveling the effects of arbuscular mycorrhizal fungi on cadmium uptake and detoxification mechanisms in perennial ryegrass (Lolium perenne)

**Authors:** Han Y, Zveushe OK, Dong FQ and more...

**Source:** Science of the Total Environment 798, 2021, DOI 10.1016/j.scitotenv.2021.149222

**Abstract:** Cadmium (Cd) is a major environmental pollutant and one of the most toxic metals in the environment. Arbuscular mycorrhizal fungi (AMF) assisted phytoremediation can be used to remove Cd from polluted soils but the role of AMF, which mediate in Cd accumulation and tolerance, remains poorly understood. Here we inoculated Lawn perenne with two different AMF species (*Glomus Minimum* and *Glomus mosseae*). Mycorrhizal *L. perenne* and non-mycorrhizal controls were exposed to Cd stress and we tested the effects of AMF mycorrhization on Cd uptake and subsequent tolerance, as well as the underlying mechanisms.

## Effect of the natural establishment of two plant species on microbial activity, on the composition of the fungal community, and on the mitigation of potentially toxic elements in an abandoned mine tailing

**Authors:** Solis-Hernandez AP, Chavez-Vergara BM, Rodriguez-Tovar AV and more...

**Source:** Science of the Total Environment 802, 2021, DOI 10.1016/j.scitotenv.2021.149788

**Abstract:** In Mexico, millions of tons of mining wastes are deposited in the open pit. Their content in potentially toxic elements (PTE) represents an environmental risk. In the tailings,

pioneer plant communities are established, associated with a determined diversity of fungi; plants, and fungi are fundamental in the natural rehabilitation of mining wastes. The objective was to evaluate the impact of the natural establishment of two plant species on the microbial activity, on the composition of the fungal community, and on the mitigation of the effect of PTE in a contaminated mine tailing...

## New Associations of Aerobic Bacteria that Actively Decompose Lindane

**Authors:** Nazarova EA, Egorova DO, Anan'ina LN and more...

**Source:** Applied Biochemistry and Microbiology 57, 5:643-655, 2021, DOI 10.1134/S0003683821050112

**Abstract:** Associations of aerobic bacteria capable of decomposing the organochlorine pesticide lindane at a concentration of 0.1 g/L in a mineral medium for 30-180 days were obtained via selection. It was found that the lindane-degrading associations L2-6, L3-6, L4-6, L6-6, and L4-10 were characterized by a low level of species diversity (Shannon index of 1.88-2.46)...

## Evaluation of Cr(VI) Reduction Using Indigenous Bacterial Consortium Isolated from a Municipal Wastewater Sludge: Batch and Kinetic Studies

**Authors:** Kholisa B, Matsena M, Chirwa EMN

**Source:** Catalysts 11, 9, 2021, DOI 10.3390/catal11091100

**Abstract:** Hexavalent Chromium (Cr(VI)) has long been known to be highly mobile and toxic when compared with the other stable oxidation state, Cr(III). Cr(VI)-soluble environmental pollutants have been detected in soils and water bodies receiving industrial and agricultural waste. The reduction of Cr(VI) by microbial organisms is considered to be an environmentally compatible, less expensive and sustainable remediation alternative when compared to conventional treatment methods, such as chemical neutralization and chemical precipitation of Cr.
This study aims to isolate and identify the
composition of the microbial consortium culture
isolated from waste activated sludge and digested
sludge...

Isolation and identification of
arsenic resistant bacteria: a
tool for bioremediation of
arsenic toxicity

Authors: Mandal D, Sonar R, Saha I and more...

Source: International Journal of Environmental
Science and Technology, 2021, DOI
10.1007/s13762-021-03673-9

**Abstract:** The soil and groundwater of Asanpara village (Bhagobangola I block) of Murshidabad district are contaminated with non-permissible limit of arsenic and other elements that co-exist with arsenic in various chemical compounds like arsenopyrite, ferrous arsenate, ferric arsenate, etc. Arsenic resistant bacteria (ARB) were isolated from arsenic contaminated soil of Asanpara and biochemically characterized...

Paracoccus and
Achromobacter bacteria
contribute to rapid
biodegradation of
imidacloprid in soils

Authors: Gao YX, Liu M, Zhao XY and more...

Source: Ecotoxicology and Environmental Safety
225, 2021, DOI 10.1016/j.ecoenv.2021.112785

**Abstract:** Neonicotinoids are among the most widely used insecticides worldwide, and as such, have garnered increasing attention from the scientific community in regards to their potentially negative environmental impacts. Recently, the degradability of neonicotinoid in soil has gained more attentions. However, what role soil microbes play in this degradation remains vastly underexplored. In this study, we compared the capacity of soil microbes sampled from different geographic regions and fields to degrade the neonicotinoid insecticide imidacloprid...

The intercropping and
arbuscular mycorrhizal
fungus decrease Cd
accumulation in upland rice
and improve
phytoremediation of Cd-
contaminated soil by
*Sphagneticola calendulacea*
(L.) Pruski

Authors: Lei LL, Zhu QY, Xu PX, Jing YX

Source: Journal of Environmental Management
98, 2021, DOI 10.1016/j.jenvman.2021.113516

**Abstract:** Little is known about the impact of the combined application of intercropping and arbuscular mycorrhizal fungus (AMF) on the plant growth and Cd accumulation in the two intercropped plants. A greenhouse pot experiment was performed to investigate the effects of intercropping (IC) and AMF-*Glomus versiforme* (GV) on the growth, photosynthesis, Cd accumulation and antioxidant activities in the two intercropped plants-upland rice and Cd hyperaccumulator *Sphagneticola calendulacea* (L.) Pruski in the soils added with 5 mg Cd kg (-1)...

Optimisation of Various
Physicochemical Variables
Affecting Molybdenum
Bioremediation Using
Antarctic Bacterium,
Arthrobacter sp. Strain AQ5-05

Authors: Darham S, Syed-Muhaimin SN,
Subramaniam K and more...

Source: Water 13, 17, 2021, DOI
10.3390/w13172367

**Abstract:** The versatility of a rare metal, molybdenum (Mo) in many industrial applications is one of the reasons why Mo is currently one of the growing environmental pollutants worldwide. Traces of inorganic contaminants, including Mo, have been discovered in Antarctica and are