# Data on the expression of GSTE1 and GSTE7 in Drosophila chemosensory organs after isothiocyanate exposure

Stéphane Fraichard, Daniel Gonzalez, Paul Grassein, Patrice Delarue, Patrick

Senet, Adrien Nicolaï, Evelyne Chavanne, Elodie Mucher, Yves Artur,

Jean-François Ferveur, et al.

► To cite this version:

Stéphane Fraichard, Daniel Gonzalez, Paul Grassein, Patrice Delarue, Patrick Senet, et al. Data on the expression of GSTE1 and GSTE7 in Drosophila chemosensory organs after isothiocyanate exposure. Data in Brief, 2018, 20, pp.254-257. 10.1016/j.dib.2018.07.062. hal-02624391

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Data in Brief

journal homepage: www.elsevier.com/locate/dib

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Data Article

# Data on the expression of *GSTE1* and *GSTE7* in Drosophila chemosensory organs after isothiocyanate exposure

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CrossMark

Stéphane Fraichard<sup>a</sup>, Daniel Gonzalez<sup>a</sup>, Paul Grassein<sup>b</sup>,
Patrice Delarue<sup>b</sup>, Patrick Senet<sup>b</sup>, Adrien Nicolaï<sup>b</sup>,
Evelyne Chavanne<sup>a</sup>, Elodie Mucher<sup>a</sup>, Yves Artur<sup>a</sup>,
Jean-François Ferveur<sup>a</sup>, Jean-Marie Heydel<sup>a</sup>, Loïc Briand<sup>a</sup>,
Fabrice Neiers<sup>a,\*</sup>

<sup>a</sup> Centre des Sciences du Goût et de l'Alimentation (CSGA), Université de Bourgogne Franche-Comté, INRA, CNRS, France

<sup>b</sup> Laboratoire Interdisciplinaire Carnot de Bourgogne, UMR 6303 CNRS-Univ. Bourgogne Franche-Comté, 9 Av. A. Savary, BP 47 870, F-21078 Dijon Cedex, France

ARTICLE INFO

Article history:
Received 20 March 2018
Received in revised form
4 July 2018
Accepted 25 July 2018
Available online 31 July 2017

ABSTRACT

The data presented in this article are related to the research article entitled "Characterization of a Drosophila glutathione transferase involved in isothiocyanate detoxification." (Gonzalez et al., 2018) [1]. This article includes the expression level of *Drosophila melanogaster* *GSTE1* and *GSTE7* in chemosensory male tissues and the expression level of the mRNAs coding for the same enzymes after a PEITC exposure in food.

© 2018 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license

(http://creativecommons.org/licenses/by/4.0/).

DOI of original article: https://doi.org/10.1016/j.ibmb.2018.03.004

* Correspondence to: CSGA, 17 rue Sully, 21065 Dijon, France.
E-mail address: fabrice.neiers@u-bourgogne.fr (F. Neiers).

https://doi.org/10.1016/j.dib.2018.07.062

2352-3409/© 2018 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
<table><caption>**Specifications Table**</caption><tr><td>Subject area</td><td>Biology</td></tr><tr><td>More specific subject area</td><td>Toxicology</td></tr><tr><td>Type of data</td><td>Figure</td></tr><tr><td>How data was acquired</td><td>RT-qPCR</td></tr><tr><td>Data format</td><td>Analyzed</td></tr><tr><td>Experimental factors</td><td>Chemosensory organs were prepared from flies exposed or no to PEITC.</td></tr><tr><td>Experimental features</td><td><i>Drosophila melanogaster</i> antennae, palps, labellum and forelegs</td></tr><tr><td>Data source location</td><td>Dijon, France</td></tr><tr><td>Data accessibility</td><td>Data are supplied with this article</td></tr></table>

**Value of the data**

* The data presented in this article show that *GSTE1* and *GSTE7* mRNA are expressed in male chemosensory tissues.

* *GSTE1* and *GSTE7* mRNA expression is significantly higher in antennae and palps compared to heads. *GSTE1* mRNA expression is higher in labellum and forelegs compared to Drosophila heads.

* A three day-long exposure to food containing PEITC led to a significant increase of *GSTE7* mRNA expression in taste organs but did not significantly change *GSTE1* mRNA expression in chemosensory tissues.

**1. Data**

The data shown here describe the *GSTE1* and *GSTE7* mRNA expression in Drosophila male chemosensory organs and are related to the research article entitled “Characterization of a Drosophila glutathione transferase involved in isothiocyanate detoxification.” (Gonzalez et al., 2018) [1]. The relative amount of mRNAs coding for *GSTE1* and *GSTE7* showed a higher expression level in olfactory organs (antennae and palps) compared to fly heads (Fig. 1). After a three day-long exposure to food containing phenethyl isothiocyanate (PEITC), only the *GSTE7* mRNA expression level was changed (Fig. 2). This exposure led to an increased expression in the labellum and forelegs.

**2. Experimental design, materials and methods**

**2.1. *Drosophila* strains, rearing conditions and ITC treatments**

For this study, we used Canton-S (Cs) wild-type male flies. Flies were reared on standard yeast/cornmeal/agar medium in a humidified, temperature-controlled incubator at 25 °C under a 12 h light: 12 h dark cycle.

PEITC (CAS no. 2257-09-2) was dissolved in ethanol (final concentration of PEITC was 0.25 mM) and added to the media at 50 °C (Merck, Kenilworth, New Jersey, USA). A similar volume of ethanol was added for both the experimental and drug-free control tests. Flies were transferred to experimental treatments at a density of 10 per vial. 30 flies were used in each treatment and they were exposed to experimental treatments during 3 successive days.

**2.2. RNA extraction and RT-qPCR**

Total RNA was extracted using Isol RNA Lysis reagent (5Prime) and was treated with RNAse-free DNAse (Euromedex, Souffelweyersheim, France) to avoid genomic DNA contamination. Total RNA was
<figure><img src="image_5.png" /><figcaption>**Fig. 1.** Expression level of *D. melanogaster* *GSTE1* and *GSTE7* in male chemosensory tissues. Real time PCR analysis was performed using RNA extracted from olfactory appendages (antennae and palps), taste appendages (labellum and forelegs) and heads deprived of chemosensory appendages. The numbers shown on the y-axis represent arbitrary units indicating relative level of the RNAs.</figcaption></figure>

reverse-transcribed using the iScript cDNA Synthesis Kit (BioRad, Hercules, USA). The qPCR reactions were carried out on a MyIQ (BioRad, Hercules, USA) using the IQ SYBR Green SuperMix (BioRad, Hercules, USA). Each reaction was performed in triplicate. All results were normalized relatively to the tubulin and rp-49 mRNA levels and the relative amount of mRNAs were calculated using the ΔΔCt method.

All error bars represent SEMs. REST Software was used to compare qPCR sets of data. Asterisks indicate the level of statistical significance (* p < 0.05, ** p < 0.01, *** p < 0.001) [2].
<figure><img src="image_6.png" /><figcaption>**Fig. 2.** Effects of PEITC on expression of *GSTE1* and *GSTE7* RNAs in chemosensory organs of adult male flies. Relative expression of *GSTE1* and *GSTE7* RNAs in olfactory appendages (antennae + palps), in taste appendages (labellum + forelegs) from Drosophila males exposed to PEITC (0.25 mM) or control. The numbers shown on the y axis are arbitrary units indicating relative level of the RNAs.</figcaption></figure>

**Acknowledgements**

This work was supported by the "Agence Nationale de la Recherche" (ANR), located at 50 avenue Daumesnil, Paris, France (75012), Grant no. ANR-16-CE21-0004-01.

**Transparency document. Supplementary material**

Transparency document associated with this article can be found in the online version at https://doi.org/10.1016/j.dib.2018.07.062.

**References**

[1] D. Gonzalez, S. Fraichard, P. Grassein, P. Delarue, P. Senet, A. Nicolaï, E. Chavanne, E. Mucher, Y. Artur, J.F. Ferveur, J.M. Heydel, L. Briand, F. Neiers, Characterization of a Drosophila glutathione transferase involved in isothiocyanate detoxification, Insect Biochem. Mol. Biol. 95 (2018) 33–43.

[2] M.W. Pfaff, G.W. Horgan, L. Dempfle, Relative expression software tool (REST) for group-wise comparison and statistical analysis of relative expression results in real-time PCR, Nucleic acids Res. 30 (2002) e36.