# A promising probiotic capable to reduce the impact of undernutrition on the development of sarcopenia

Muriel Giron, Muriel Thomas, Stéphanie Bornes, Marianne Jarzaguet, Camille Mayeur, Dominique Dardevet, Christophe Chassard, Isabelle Savary-Auzeloux

► To cite this version:

Muriel Giron, Muriel Thomas, Stéphanie Bornes, Marianne Jarzaguet, Camille Mayeur, et al. A promising probiotic capable to reduce the impact of undernutrition on the development of sarcopenia. 44th ESPEN Congress, European Society for Clinical Nutrition and Metabolism (ESPEN), Sep 2022, Vienna, Austria. **hal-04194999**

HAL Id: hal-04194999

https://hal.science/hal-04194999v1

Submitted on 6 Sep 2023

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Distributed under a Creative Commons CC BY 4.0 - Attribution - International License
# A Promising Probiotic Capable to Reduce the Impact of Undernutrition on the Development of Sarcopenia

GIRON M.<fnref n="1" />⁻<fnref n="2" />, THOMAS M.<fnref n="3" />, BORNES S.<fnref n="2" />, JARZAGUET M.<fnref n="1" />, MAYEUR C.<fnref n="3" />, DARDEVET D.<fnref n="1" />, CHASSARD C.<fnref n="2" /> and SAVARY-AUZELOUX I.<fnref n="1" />

Contact: muriel.giron@inrae.fr

## INTRODUCTION

**Sarcopenia** in undernourished elderly (>20% of hospitalized patients) increases the risk of morbidity and mortality [1].

Other target responsible of sarcopenia and potentiate by denutrition have to be identified to optimize and favor multimodal strategies.

<figure><img src="image_2.png" /></figure>

The **gut microbiota**, being shown to be altered during aging and denutrition, could represent an interesting and innovative target to consider in the management of sarcopenia [2].

**Objective:** to select a probiotic bacteria capable to limit muscle mass loss in a undernourished old rodent model (a model of frail old population).

## METHODS

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(1) Isolation of lactic acid bacteria (LAB) from the gut microbiota of patient with short bowel syndrome (SBS), a population that presents adaptations to optimize food efficiency [3];

(2) screening of isolated bacteria on *Caenorhabditis elegans* model on lifespan and muscle integrity ;

(3) evaluation of daily supplementation of promising strain (~10⁹) in old rats (20 mo. ♂) under mild food restriction (75% ad libitum) for 1 month.

<figure><img src="image_4.png" /></figure>

## RESULTS

### (1) *C. elegans* lifespan

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LC63 increased worm longevity compared to worms fed a control diet (E. coli OP50) and other strains.

Log-Rank test, p<0.001

### (3) Muscle protein content and turnover

<figure><img src="image_6.png" /></figure>

LC63 counteracted muscle protein loss following 1 month of treatment.

Kruskal-Wallis and Dunn's tests, p<0.05

LC63 maintained postprandial activation of Akt/mTOR/S6 pathway despite food restriction.

Kruskal-Wallis and Dunn's tests, p<0.05

### (2) *C. elegans* body wall muscle integrity

<figure><img src="image_7.png" /></figure>

LC63 limited the fragmentation of muscle mitochondrial network over time. p<0.001

<figure><img src="image_8.png" /></figure>

No significant impact on proteolysis pathways.

Kruskal-Wallis and Dunn's tests, p>0.05

## CONCLUSION

LC63 (patented strain) prevented muscle mass loss and could represent an interesting strategy to limit sarcopenia.

Future work:

Use of LC-5663 in supplementation strategies in humans presenting sarcopenia.

⇒ Clinical study in old populations required.

Pictures: BioRender.com

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<footnote n="1">¹ Université Clermont Auvergne, INRAE UMR 1019, Unité de Nutrition Humaine, Clermont-Ferrand, France</footnote>

<footnote n="2">² Université Clermont Auvergne, INRAE UMR 0545, Unité Mixte de Recherche sur le Fromage, Aurillac, France</footnote>

<footnote n="3">³ Université Paris-Saclay, INRAE UMR 1319, Institut MICALIS, Jouy-en-Josas, France</footnote>

<footnote>[1] Cerri et al., 2015. Clin Nutr</footnote>

<footnote>[2] Giron et al., 2022. J Cachexia Sarcopenia Muscle.</footnote>

<footnote>[3] Gillard et al., 2017. Front. Physiol.</footnote>

<footnote>[4] Gaffney et al., 2018. Aging</footnote>