# Phytochemical screening of Pentadesma butyracea Sabine (Clusiaceae) acclimated in Benin by GC/MS

J. P. A. Noudogbessi, A. K. Natta, F. P. Tchobo, G. S. Bogninou, F. T. D. Bothon, A. D. Bossou, G. Figueredo, P. Chalard, J. C. Chalchat, D.C. K. Sohounhloué

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J. P. A. Noudogbessi, A. K. Natta, F. P. Tchobo, G. S. Bogninou, F. T. D. Bothon, et al., Phytochemical screening of Pentadesma butyracea Sabine (Clusiaceae) acclimated in Benin by GC/MS. ISRN Analytical Chemistry, 2013, 2013, pp.172397. 10.1155/2013/172397. hal-00950211

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Research Article

# Phytochemical Screening of *Pentadesma butyracea* Sabine
(Clusiaceae) Acclimated in Benin by GC/MS

J. P. A. Noudogbessi,<fnref n="1" /> A. K. Natta,<fnref n="2" /> F. P. Tchobo,<fnref n="1" /> G. S. Bogninou,<fnref n="1" /> F. T. D. Bothon,<fnref n="1" />
A. D. Bossou,<fnref n="1" /> G. Figueredo,<fnref n="3" /> P. Chalard,<fnref n="4" /> J. C. Chalchat,<fnref n="5" /> and D. C. K. Sohounhloué<fnref n="1" />

Correspondence should be addressed to D. C. K. Sohounhloué; csohoun@gmail.com

Received 27 December 2012; Accepted 27 January 2013

Academic Editors: R. K. Jyothi, T. Macko, and W. X. Misiuk

Copyright © 2013 J. P. A. Noudogbessi et al. This is an open access article distributed under the Creative Commons Attribution
License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly
cited.

The results brought back at the end of this work concerned various chemical constituents of *P. butyracea* materials collected in seven forest galleries in northern of Benin. The phytochemical analysis showed mucilage, coumarins, gallic tannins, flavones, sterols, and saponins, in its leaves. The cyclohexanic fractions realized from petroleum ether extracts and analysed by GC/MS were marked by important rates of 9, 19-cyclolanost-24-en-3β-3-ol (49.3–72.6%), taraxasterol (18.4–30.1%), and friedooleanan-3-one (10.0%). Essential oils extracted by hydrodistillation from *P. butyracea* and analyzed by GC/MS contained 11 to 38 compounds representing 85.2 to 99.5% of the weight of this volatile extracts essentially rich in sesquiterpene constituents. The essential oils predominant compounds (>10%) identified and recorded independently of the organ studied were β-caryophyllene (14.9–77.9%), aromadendrene (43.5%), α-copaene (18.4–26.6%), α-ylangene (21.1%), germacrene-B (5.1–13.5%), selina-3,7(11)-diene (13.3%), α-humulene (6–13.3%), (2E, 6Z)-α-farnesene (12.6%), seychellene (12.0%), and palmitic acid (10,6%).

**1. Introduction**

The food forest tree species were plentiful in African forest
ecosystems, and they contributed to the household economy,
to the strengthening food security, and to the preservation
of the biological diversity of forest resources [1]. In Benin,
some of these woody species including *P. butyracea* were
threatened with a severe extinction due to the lack of a real
program of long-lasting management of their ecosystems [2].
*P. butyracea* is a dense forest species with a distribution area
reaching from Sierra Leone to the Cameroun [3]; it is a
tree, with a height of about 20 m, which was found in the
north of Benin in forest galleries and along water way [4].
The bark, rough, and deeply cracked exudes a thick resinous

juice, of reddish yellow color. The leaves were 10–22 cm long,
3.5–7 cm broad, with numerous close parallel, lateral nerves;
the flowers were large, white, or sometimes red-dish; the
fruits were broadly ellipsoid, pointed, about 15 cm long and
10 cm large [5]; they contained oleaginous almonds which
were consumed like kola [6] and were used to extract an
edible butter (named kanga or lamy butter) [7], similar to
Shea butter (Butyrospermum parkii Katschy, Sapotaceae). *P.
butyracea* butter was used in traditional medicine as massage
oil, in skin and hair care, and in the manufacture of soap for
its softening, lubricating, and healing qualities [8]. It was used
to retard the ageing of skin in patented cosmetic preparation
[9]. In Gabon, the macerated bark was used in lotions for
the treatment of the parasitic diseases of the skin and as

<footnote n="1">¹ Laboratoire d'Etude et de Recherche en Chimie Appliquée (LERCA), Ecole Polytechnique d'Abomey-Calavi, 01 BP 2009 Cotonou, Benin</footnote>

<footnote n="2">² Faculté d'Agronomie, Laboratoire d'Etudes et de Recherches Forestières (LERF), Université de Parakou, BP 123 Parakou, Benin</footnote>

<footnote n="3">³ Laboratoire d'Analyse des Extraits Végétaux et des Arômes (LEXVA Analytique), 460 rue du Montant, 63110 Beaumont, France</footnote>

<footnote n="4">⁴ Institut de Chimie de Clermont-Ferrand (ICCF), Ecole Nationale Supérieure de Chimie de Clermont-Ferrand (ENSCCF), BP 10448, 63000 Clermont-Ferrand, France</footnote>

<footnote n="5">⁵ Laboratoire de Chimie des Huiles Essentielles, Université Blaise-Pascal, (Clermont-Ferrand II), Campus des Cézeaux, 63177 Aubière Cedex, France</footnote>
antidiarrheal [10]. In Ghana, the roots decoction was used to fight intestinal worms [11]. The social and economic value of this plant species made it a known tree and potentially used for multiple purposes. Indeed, its organs (almonds, leaves, flowers, bark, and roots) were used in food, cosmetic, and therapeutic practices [6, 11]. Previously, the chemical investigations realized by Alitonou et al. showed that the essential oils extracted from leaves, bark, and roots of *P. butyracea* harvested to Natitingou in Benin contained high levels of hydrogenated sesquiterpenes, mainly dominated by the *β*-caryophyllene (58–75%) [12]. In 2007, Tchobo et al. reported that the content of stearic and oleic acids in butter extracted from *P. butyracea* seeds were 96.0%. They also signified the presence of this butter of sterols (68.0%) whose predominants were stigmasterol and *β*-tocopherol [13]. This work was thus a contribution to the recovery of this species (*Pentadesma butyracea* Sabine (Clusiaceae)) endangered in Benin by the highlighted thanks to modern technical and appropriate analysis of the chemical constituents, possibly bioactive. It also aims to verify if *P. butyracea* was chemically constant in each of its parts taken by studying the volatile compounds extracted from its various parts resulting from several localities of Benin.

## 2. Experimental

2.1. *Plant Material, Oil Isolation, and Obtaining Powders.* The *P. butyracea* vegetal materials were collected in June-July 2006 in the forest galleries of Agbassa, Bakabaka, Bassila, Natitingou-Ville, Penelan, and Penessoulou situated in the northern of Benin. They were authenticated at the National Herbarium of Abomey-Calavi University. In the laboratory, these materials were kept between 18 and 20°C in the shade of sunlight throughout the study period. The essential oils were obtained in pentane from 750 g of leaves, trunk bark, roots, and root bark by using hydrodistillation technic during 8 h with a Clevenger (Type Apparatus), according to the British pharmacopoeia method [14]. The pentane was evaporated at ambient temperature. They were dried over anhydrous sodium sulfate and analyzed by GC/FID and GC/MS. For the determination of nonvolatile compounds, powders were obtained from the leaves, dried in the dark for one month, by grinding with a knives machine Ika Werke MF 10 basic type. Vegetable powders collected are then sieved in the size grading 0.425.

2.2. *Nonvolatile Constituent Identification of P. butyracea Leaves.* The phytochemical screening was made according to the standard techniques described by Paris and Moyse [15], Bouquet [16], Debray et al. [17], and Harborne [18]. *Mucilages.* 1 mL of decoction realized previously was treated with 5 mL of absolute ethanol, and the presence of mucilage was noticed by the appearance of a flaky precipitate.

*Coumarins.* An infusion was made from 10 g of powder and 100 mL of ethanol. The alcoholic extract obtained was

examined under UV light (365 nm). The appearance of a bluish fluorescence indicated a positive reaction.

*Tannins Gallic.* An aqueous infusion was prepared from 5 g of plant powder and 100 mL of boiling distilled water. After 15 min, the mixture was filtered. The residue was rinsed with hot water to bring the volume of the filtrate to 100 mL. 20 mL of the filtrate is saturated with sprayed sodium sulfate, and then, it was added dropwise 1 mL of ferric chloride (1%). The development of a blue-black tint corresponded to the presence of gallic tannins, not precipitated by Stiasny's reagent.

*Flavones.* They were introduced in a test tube, 5 mL of the infused. In this content, 5 mL of hydrochloric alcohol constituted by equal volumes of ethanol at 95°, distilled water, concentrated hydrochloric acid (37%), and 1 mL of isoamylic alcohol was added. In the presence of shavings magnesium, it emerged at the supernatant layer (layer isoamyl alcohol) a pink-orangey color indicating the presence of genins of flavonoids.

*Leucoanthocyanins.* They were identified by being introduced into a test tube 5 mL of infused (5%) and 5 mL of hydrochloric (ethanol 95° + distilled water + hydrochloric acid 37% of equal volumes). The mixture was competed with 1 mL of isoamylic alcohol and then heated to 90° through a water bath. After fifteen minutes, it had developed a red-cerise tint (or purple) indicating the presence of leucoanthocyanins.

*Saponins.* A decoction was prepared during 30 min from two grams of plant powder and 100 mL of distilled water. After filtering the obtained mixture, the filtrate was divided into 10 different volumes (1 mL, 2 mL, 3 mL, and 10 mL) in 10 calibrated tubes (internal diameter : 1.3 cm). The content of each tube was adjusted to 10 mL with distilled water. After shaking each tube in a horizontal position for 15 seconds, followed by a rest of 15 min in an upright position, the height of the foam supernatant was measured in cm. When this height is close to 1 cm in the Xth tube, the foam index (I) is calculated by the following formula: I = foam height (in cm) in the Xth tube × 5/0.0X. The presence of saponins in the plant is confirmed when the value of the foam index is greater than 100.

*Sterols and Triterpenes (Liebermann-Burchard Test).* It was proceeded to the depigmentation of 100 mg of powder hydroalcoholic extract of *P. butyracea* with 10 mL of cyclohexane under hanging excitement for 5 min. The depigmented residue is then treated with 10 mL of chloroform. The recovered solution, dried over sodium sulfate anhydrous, was filtered, and the filtrate obtained was divided into two test tubes. In the first tube, were introduced three drops of acetic anhydride. After gentle stirring, it was added a drop of concentrated sulfuric acid. One hour later, the appearance of a blue-green color indicates the presence of steroids or triterpenes if it changes from red-purple to pink. In the second tube, no color change was observed.

*Free Anthraquinones.* They were carried out with 1 g of plant material powder and 10 mL of chloroform. Afterward, 1 mL of the extract filtrate was shaken with 1 mL of NH₄OH diluted
<table><caption>TABLE 1: Chemical families identified in *P. butyracea* leaves.</caption><thead><tr><th>Mucilage</th><th>Free antraquinones</th><th>Coumarin</th><th>Gallic tannins</th><th>Flavones</th><th>Leucoanthocyanines</th><th>Saponins (foam index)</th><th>Sterols and triterpenes</th></tr></thead><tbody><tr><td>+++</td><td>+</td><td>+++</td><td>+++</td><td>++</td><td>+</td><td>++ (50.0)</td><td>++</td></tr></tbody></table>

+++: abundant; ++: average; +: very little.

<table><caption>TABLE 2: Chemical composition of unsaponifiable extracts of *P. butyracea* organs.</caption><thead><tr><th>Compounds</th><th>KI</th><th>L</th><th>TB</th><th>R</th><th>RB</th></tr><tr><td></td><td></td><td></td><td>%</td><td></td><td></td></tr></thead><tbody><tr><td>α-cubebene</td><td>1351</td><td>—</td><td>0.1</td><td>0.1</td><td>0.1</td></tr><tr><td>α-copaene</td><td><strong>1380</strong></td><td><strong>4.3</strong></td><td>0.2</td><td>0.2</td><td>0.1</td></tr><tr><td>cyperene</td><td>1411</td><td>—</td><td>—</td><td>0.7</td><td>0.2</td></tr><tr><td>β-caryophyllene</td><td><strong>1425</strong></td><td>0.4</td><td><strong>6.9</strong></td><td><strong>7.8</strong></td><td><strong>4.4</strong></td></tr><tr><td>trans-α-bergamotene</td><td>1438</td><td>—</td><td>—</td><td>0.1</td><td>—</td></tr><tr><td>allo-aromadendrene</td><td>1464</td><td>—</td><td>1.1</td><td>1.3</td><td>0.8</td></tr><tr><td>α-selinene</td><td>1491</td><td>0.4</td><td>—</td><td>0.1</td><td>—</td></tr><tr><td>β-bisabolene</td><td>1512</td><td>—</td><td>0.3</td><td>0.3</td><td>0.2</td></tr><tr><td>δ-cadinene</td><td>1522</td><td>0.7</td><td>0.1</td><td>0.1</td><td>0.1</td></tr><tr><td>Caryolan-8-ol</td><td>1584</td><td>—</td><td>—</td><td>0.1</td><td>—</td></tr><tr><td>Caryophyllene oxide</td><td>1590</td><td>—</td><td>0.2</td><td>0.1</td><td>0.1</td></tr><tr><td>6,10,14-trimethylpentadecan-2-one</td><td>1837</td><td>1.2</td><td>—</td><td>—</td><td>—</td></tr><tr><td>Phytol</td><td><strong>1949</strong></td><td><strong>8.5</strong></td><td>—</td><td>—</td><td>—</td></tr><tr><td>Dibutyl phthalate</td><td>2142</td><td>—</td><td>0.2</td><td>0.2</td><td>—</td></tr><tr><td>Cyclododecane</td><td>2469</td><td>—</td><td>—</td><td>—</td><td>0.2</td></tr><tr><td>cis-octadec-13-enal</td><td>2475</td><td>—</td><td>—</td><td>—</td><td>0.2</td></tr><tr><td>squalene</td><td>2824</td><td>2.6</td><td>0.1</td><td>—</td><td>—</td></tr><tr><td>nonacosane</td><td><strong>2878</strong></td><td><strong>3.7</strong></td><td>—</td><td>—</td><td>—</td></tr><tr><td>β-tocopherol</td><td>3028</td><td>0.5</td><td>—</td><td>—</td><td>—</td></tr><tr><td>γ-tocopherol</td><td>3036</td><td>0.6</td><td>—</td><td>—</td><td>—</td></tr><tr><td>Hentriacontane</td><td><strong>3099</strong></td><td><strong>3.3</strong></td><td>—</td><td>—</td><td>—</td></tr><tr><td>α-tocopherol</td><td>3118</td><td>1.0</td><td>—</td><td>—</td><td>—</td></tr><tr><td>δ-5-ergosterol</td><td>3204</td><td>0.6</td><td>—</td><td>—</td><td>—</td></tr><tr><td>Lanosta-8,24-dien-3β-3-ol</td><td>3231</td><td>1.7</td><td>0.8</td><td>1.3</td><td>0.4</td></tr><tr><td>9,19-cyclolanost-24-en-3β-3-ol</td><td><strong>3260</strong></td><td><strong>49.3</strong></td><td><strong>55.0</strong></td><td><strong>54.7</strong></td><td><strong>72.6</strong></td></tr><tr><td>Hop-22(29)-en-3β-ol</td><td>3328</td><td>2.6</td><td>—</td><td>—</td><td>—</td></tr><tr><td>9,19-cyclolanost-23-en-3β-3,25-diol</td><td>3332</td><td>2.6</td><td>—</td><td>—</td><td>—</td></tr><tr><td>Lupeol</td><td>3338</td><td>—</td><td>2.6</td><td>—</td><td>—</td></tr><tr><td>Friedooleanan-3-one</td><td><strong>3432</strong></td><td><strong>10.0</strong></td><td>—</td><td>—</td><td>—</td></tr><tr><td>3-oxo-friedooleanan-28-al</td><td>3672</td><td>0.7</td><td>—</td><td>—</td><td>—</td></tr><tr><td>Taraxasterol</td><td><strong>3386</strong></td><td>—</td><td><strong>30.1</strong></td><td><strong>21.3</strong></td><td><strong>18.4</strong></td></tr><tr><td>Lup-20(29)-ene-3,28-3β-diol</td><td>3680</td><td>—</td><td>0.5</td><td>—</td><td>—</td></tr><tr><td>Total</td><td></td><td>94.7</td><td>98.2</td><td>88.4</td><td>97.8</td></tr></tbody></table>

L: leaves, TB: trunk barks, R: roots, RB: root barks, KI: kovats index.

to 50%. A more or less intense coloration indicating the presence of the free antraquinones was observed.

2.3. *P. butyracea* Fatty Acids (FA) and Unsaponifiable (Un) Identification. Lipids Extraction. 15.0 g of the vegetable material powder was twice extracted successively by 100 mL of petroleum ether (40–60°C) with magnetic stirring at room temperature. After filtration and evaporation of the solvent under reduced pressure, the extracts were dried and weighed.

The yields were established in calculating the average of three extractions.

Saponification and Fatty Acids Obtention. The saponification was conducted by refluxing, during thirty minutes past one o'clock, 0.5 g of plant extract, and 25 mL of an ethanolic and potassium hydroxide solution (2N). After cooling, 50 mL of water was added and the unsaponifiable matter is extracted by 3 × 50 mL of cyclohexane. The soap solution produced was
<table><caption>TABLE 3: Essential oils composition (%) of leaves, trunk bark, roots, and root barks of *P. butyracea* Sabine.</caption><thead><tr><th>Harvest locality<br>P. butyracea part<br>yield (×10<sup>-2</sup>%)</th><th>A<br>TB<br>3.5 ± 0.1</th><th>B<br>L<br>7.8 ± 1.2</th><th>L<br>1.4 ± 0.0</th><th>B<sub>1</sub><br>2.8 ± 0.1</th><th>TB<br>3.2 ± 0.2</th><th>B<sub>2</sub><br>2.6 ± 0.1</th><th>L<br>50.1 ± 10.2<br>%</th><th>NV<br>TB<br>37.0 ± 1.0</th><th>R<br>7.0 ± 0.1</th><th>RB<br>23.0 ± 0.2</th><th>Pn<br>L<br>4.0 ± 0.1</th><th>Pu<br>L<br>2.0 ± 0.1</th></tr></thead><tbody><tr><td>Compounds</td><td>KI</td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td><td></td></tr><tr><td>Sabinene</td><td>968</td><td>—</td><td>0.1</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>0.1</td></tr><tr><td>oct-1-en-3-ol</td><td>978</td><td>—</td><td>—</td><td>0.1</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td></tr><tr><td>6-methyl-5-hepten-2-one</td><td>982</td><td>0.1</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td></tr><tr><td>Myrcene</td><td>991</td><td>—</td><td>—</td><td>1.4</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>0.1</td><td>—</td></tr><tr><td>p-cymene</td><td>1019</td><td>—</td><td>0.1</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td></tr><tr><td>Limonene</td><td>1024</td><td>—</td><td>t</td><td>0.2</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>0.1</td><td>—</td><td>—</td></tr><tr><td>1,8-cineole</td><td>1033</td><td>—</td><td>0.6</td><td>0.1</td><td>—</td><td>0.1</td><td>t</td><td>—</td><td>—</td><td>—</td><td>0.2</td><td>—</td></tr><tr><td>Linalool</td><td>1096</td><td>0.1</td><td>3.1</td><td>1.0</td><td>—</td><td>1.4</td><td>—</td><td>—</td><td>0.1</td><td>0.6</td><td>0.9</td><td>1.0</td></tr><tr><td>n-nonanal</td><td>1101</td><td>0.1</td><td>—</td><td>—</td><td>0.1</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>0.2</td><td>—</td></tr><tr><td>Terpinen-4-ol</td><td>1175</td><td>—</td><td>—</td><td>0.1</td><td>—</td><td>0.1</td><td>—</td><td>—</td><td>—</td><td>0.1</td><td>—</td><td>—</td></tr><tr><td>Myrtenal</td><td>1190</td><td>—</td><td>—</td><td>t</td><td>—</td><td>0.5</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td></tr><tr><td>α-terpineol</td><td>1191</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>0.1</td><td>0.1</td></tr><tr><td>Dihydro carveol</td><td>1192</td><td>—</td><td>0.2</td><td>—</td><td>—</td><td>—</td><td>t</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td></tr><tr><td>trans-dihydro carvone</td><td>1212</td><td>—</td><td>—</td><td>t</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>0.1</td><td>—</td></tr><tr><td>trans-carveol</td><td>1217</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>0.1</td></tr><tr><td>Neral</td><td>1240</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>0.3</td></tr><tr><td>1-phenyl pentan-3-one</td><td>1241</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td></tr><tr><td>Geraniol</td><td>1244</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td></tr><tr><td>Chavicol</td><td>1248</td><td>—</td><td>0.3</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td></tr><tr><td>Carvacrol</td><td>1297</td><td>—</td><td>0.1</td><td>—</td><td>0.2</td><td>2.1</td><td>—</td><td>0.2</td><td>—</td><td>—</td><td>—</td><td>—</td></tr><tr><td>α-cubebene</td><td>1341</td><td>0.1</td><td>0.2</td><td>1.7</td><td>0.4</td><td>—</td><td>—</td><td>—</td><td>0.3</td><td>0.8</td><td>0.3</td><td>0.1</td></tr><tr><td>α-longipinene</td><td>1362</td><td>—</td><td>0.4</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>0.1</td><td>—</td><td>—</td><td>—</td></tr><tr><td>Cyclosativene</td><td>1363</td><td>—</td><td>—</td><td>0.3</td><td>0.1</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td></tr><tr><td>clove</td><td>1365</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>t</td><td>0.1</td><td>—</td><td>—</td></tr><tr><td>α-ylangene</td><td><b>1370</b></td><td>—</td><td>—</td><td>—</td><td>—</td><td><b>21.1</b></td><td>—</td><td>—</td><td>0.1</td><td>0.1</td><td>0.3</td><td>8.3</td></tr><tr><td>α-copaene</td><td><b>1379</b></td><td>0.1</td><td><b>26.6</b></td><td><b>18.4</b></td><td>1.3</td><td>—</td><td>2.3</td><td>3.2</td><td>1.5</td><td>1.0</td><td>0.6</td><td><b>19.9</b></td></tr><tr><td>β-cubebene</td><td>1381</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>0.3</td><td><b>6.2</b></td><td>0.1</td><td>0.3</td><td>0.1</td><td>—</td></tr><tr><td>cyperene</td><td>1395</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>0.1</td><td>0.8</td><td>0.6</td><td>0.2</td><td>0.3</td><td>—</td></tr><tr><td>Isocaryophyllene</td><td>1414</td><td>—</td><td>—</td><td>—</td><td>—</td><td>0.5</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>1.4</td></tr><tr><td>β-caryophyllene</td><td><b>1418</b></td><td><b>77.9</b></td><td><b>43.36</b></td><td><b>56.4</b></td><td><b>36.4</b></td><td><b>47.8</b></td><td><b>72.89</b></td><td><b>41.2</b></td><td><b>67.4</b></td><td><b>64.0</b></td><td><b>64.1</b></td><td><b>41.7</b></td><td><b>14.9</b></td></tr><tr><td>β-copaene</td><td>1425</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>0.3</td><td>—</td><td>—</td><td>—</td><td>0.6</td><td>2.3</td></tr><tr><td>Aromadendrene</td><td><b>1435</b></td><td>—</td><td>—</td><td>—</td><td>43.5</td><td>—</td><td>—</td><td>—</td><td>—</td><td>0.1</td><td>0.9</td><td>—</td><td>—</td></tr><tr><td>(Z)-β-farnesene</td><td><b>1444</b></td><td>0.1</td><td>—</td><td>0.6</td><td>—</td><td>7.7</td><td>—</td><td>—</td><td>—</td><td>0.1</td><td>—</td><td>0.5</td><td>—</td></tr><tr><td>α-humulene</td><td><b>1452</b></td><td>1.2</td><td><b>6.7</b></td><td><b>8.2</b></td><td>—</td><td>—</td><td>—</td><td><b>11.6</b></td><td><b>6.0</b></td><td><b>11.6</b></td><td><b>11.1</b></td><td><b>13.3</b></td><td><b>6.9</b></td></tr><tr><td>α-neo-clorene</td><td>1454</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>0.3</td><td>0.3</td><td>0.4</td><td>0.3</td><td>—</td></tr><tr><td>Aromadendr-9-ene</td><td>1463</td><td>—</td><td>0.4</td><td>0.3</td><td>—</td><td>—</td><td>—</td><td>—</td><td>0.1</td><td>—</td><td>0.1</td><td>—</td><td>—</td></tr><tr><td>Seychellene</td><td><b>1467</b></td><td><b>12.0</b></td><td>—</td><td>—</td><td>0.2</td><td>—</td><td>0.2</td><td>—</td><td>—</td><td>0.1</td><td>—</td><td>—</td><td>—</td></tr><tr><td>α-acoradiene</td><td>1468</td><td>—</td><td>0.4</td><td>0.3</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>0.7</td><td>—</td></tr><tr><td>(2E, 6Z)-α-farnesene</td><td><b>1472</b></td><td>—</td><td>0.5</td><td>—</td><td>—</td><td><b>12.6</b></td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td></tr></tbody></table>
<table><caption>TABLE 3: Continued.</caption><thead><tr><th rowspan="2">Compounds</th><th rowspan="2">Kl</th><th colspan="10">Harvest locality</th><th colspan="2">NV</th><th rowspan="2">Pn</th><th rowspan="2">Pu</th></tr><tr><th>P. butyracea part yield (×10<sup>-2</sup>%)</th><th>A TB 3.5 ± 0.1</th><th>B L 7.8 ± 1.2</th><th>B<sub>1</sub> L 1.4 ± 0.0</th><th>B TB 2.8 ± 0.1</th><th>B<sub>2</sub> L 3.2 ± 0.2</th><th>TB 2.6 ± 0.1</th><th>L 50.1 ± 10.2</th><th>TB 37.0 ± 1.0</th><th>R 7.0 ± 0.1</th><th>RB 23.0 ± 0.2</th><th>L 4.0 ± 0.1</th><th>L 2.0 ± 0.1</th></tr></thead><tbody><tr><td>8-murolene</td><td>1475</td><td>0.1</td><td>0.8</td><td>0.1</td><td>0.2</td><td>—</td><td>—</td><td>—</td><td>—</td><td>0.2</td><td>0.2</td><td>—</td><td>0.7</td></tr><tr><td>9-epi-(E)-caryophyllene</td><td>1476</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>0.3</td><td>—</td><td>—</td><td>0.1</td><td>0.8</td><td>0.5</td><td>—</td></tr><tr><td>β-selinene</td><td>1482</td><td>0.1</td><td>—</td><td>0.3</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>0.5</td></tr><tr><td>ar-curcumene</td><td>1486</td><td>0.1</td><td>—</td><td>0.5</td><td>—</td><td>—</td><td>—</td><td>—</td><td>3.7</td><td>0.3</td><td>0.2</td><td>0.1</td><td>1.8</td></tr><tr><td>8-humulene</td><td>1490</td><td>0.2</td><td>—</td><td>—</td><td>0.2</td><td>—</td><td>—</td><td>0.1</td><td>—</td><td>—</td><td>0.2</td><td>—</td><td>—</td></tr><tr><td>trans-β-guaiene</td><td>1496</td><td>—</td><td>—</td><td>—</td><td>0.5</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td></tr><tr><td>α-selinene</td><td>1498</td><td>—</td><td>—</td><td>—</td><td>0.1</td><td>—</td><td>—</td><td>—</td><td>0.1</td><td>0.2</td><td>0.5</td><td>—</td><td>—</td></tr><tr><td>α-murolene</td><td>1499</td><td>—</td><td>0.1</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>0.4</td><td>0.5</td><td>—</td><td>—</td></tr><tr><td>(Z)-α-bisabolene</td><td>1502</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>2.8</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td></tr><tr><td>(E)-α-bisabolene</td><td>1504</td><td>1.7</td><td>—</td><td>—</td><td>1.4</td><td>—</td><td>—</td><td>—</td><td>0.1</td><td>—</td><td>—</td><td>—</td><td>—</td></tr><tr><td>(2E, 6E)-α-farnesene</td><td>1506</td><td>0.1</td><td>—</td><td>0.1</td><td>—</td><td>—</td><td>—</td><td>0.6</td><td>0.5</td><td>—</td><td>—</td><td>—</td><td>—</td></tr><tr><td>β-curcumene</td><td><b>1511</b></td><td>0.2</td><td>—</td><td>—</td><td>0.1</td><td>—</td><td>—</td><td>—</td><td>0.2</td><td><b>5.1</b></td><td><b>4.3</b></td><td>—</td><td>—</td></tr><tr><td>δ-amorphene</td><td><b>1512</b></td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td><b>4.9</b></td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td></tr><tr><td>8-cadinene</td><td><b>1517</b></td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>0.1</td><td>0.3</td><td>0.1</td><td>—</td></tr><tr><td>δ-cadinene</td><td>1520</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>0.6</td><td>1.4</td><td>1.1</td><td>1.4</td><td>—</td></tr><tr><td>trans-calamenene</td><td>1522</td><td>0.1</td><td>0.2</td><td>0.1</td><td>0.2</td><td>—</td><td>—</td><td>0.7</td><td>—</td><td>0.7</td><td>0.5</td><td>0.7</td><td>0.9</td></tr><tr><td>(E)-8-bisabolene</td><td>1528</td><td>0.1</td><td>0.5</td><td>—</td><td>—</td><td>—</td><td>—</td><td>0.2</td><td>—</td><td>—</td><td>0.3</td><td>0.6</td><td>—</td></tr><tr><td>Zonarene</td><td>1532</td><td>—</td><td>—</td><td>0.2</td><td>0.1</td><td>—</td><td>—</td><td>—</td><td>—</td><td>0.3</td><td>—</td><td>0.2</td><td>1.9</td></tr><tr><td>Selina-3,7(11)-diene</td><td>1542</td><td>—</td><td>0.6</td><td>0.1</td><td>0.1</td><td>—</td><td>—</td><td>—</td><td>—</td><td>0.1</td><td>—</td><td>0.1</td><td><b>13.3</b></td></tr><tr><td>Germacrene-B</td><td>1546</td><td>0.2</td><td>1.1</td><td>—</td><td>0.1</td><td>—</td><td>—</td><td>0.2</td><td>—</td><td>0.1</td><td>—</td><td>0.2</td><td><b>5.1</b></td></tr><tr><td>1,10-decanediol</td><td>1548</td><td>—</td><td>0.3</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td></tr><tr><td>8-calacorene</td><td>1559</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>0.2</td><td>—</td></tr><tr><td>(E)-nerolidol</td><td><b>1563</b></td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td><b>4.2</b></td><td>—</td><td>—</td><td>—</td><td>—</td><td>2.4</td><td>—</td></tr><tr><td>Caryophyllene alcohol</td><td>1574</td><td>2.3</td><td>—</td><td>—</td><td>0.1</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td></tr><tr><td>Caryophyllene oxide</td><td><b>1581</b></td><td>0.3</td><td>—</td><td><b>4.2</b></td><td>1.9</td><td>0.8</td><td>—</td><td>—</td><td>1.1</td><td><b>7.3</b></td><td>0.4</td><td>1.1</td><td>0.8</td></tr><tr><td>Cedrol</td><td>1599</td><td>0.1</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>0.2</td><td>—</td><td>—</td><td>—</td><td>0.2</td><td>—</td></tr><tr><td>Humulene epoxide II</td><td>1608</td><td>0.6</td><td>0.2</td><td>—</td><td>—</td><td>—</td><td>—</td><td>0.3</td><td>0.9</td><td>0.4</td><td>0.3</td><td>0.7</td><td>0.5</td></tr><tr><td>1-epi-cubenol</td><td>1626</td><td>—</td><td>—</td><td>0.1</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>0.3</td><td>0.3</td><td>0.7</td><td>—</td></tr><tr><td>Cubenol</td><td>1637</td><td>—</td><td>—</td><td>—</td><td>0.2</td><td>—</td><td>—</td><td>—</td><td>0.2</td><td>—</td><td>0.2</td><td>—</td><td>—</td></tr><tr><td>α-murolol</td><td>1646</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>1.1</td><td>1.1</td><td>—</td></tr><tr><td>α-cadinol</td><td>1559</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>0.3</td><td>—</td></tr><tr><td>Selin-11-en-4-α-ol</td><td>1663</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>0.1</td><td>—</td></tr><tr><td>Cadalene</td><td>1665</td><td>0.1</td><td>0.1</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>0.1</td><td>—</td></tr><tr><td>α-bisabolol</td><td>1689</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>0.2</td><td>—</td><td>—</td><td>0.2</td><td>—</td></tr><tr><td>(Z)-trans-α-bergamotol</td><td>1693</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>0.9</td></tr><tr><td>β-acoredienol</td><td>1762</td><td>0.1</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>0.5</td><td>—</td><td>—</td><td>—</td><td>0.3</td></tr><tr><td>6,10,14-trimethyl pentadecan-2-one</td><td>1840</td><td>0.1</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>0.5</td><td>—</td><td>—</td><td>—</td><td>—</td></tr><tr><td>Phytol</td><td>1946</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>0.6</td><td>—</td><td>—</td><td>—</td><td>—</td></tr><tr><td>Palmitic acid</td><td><b>1966</b></td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td><b>10.6</b></td><td>—</td><td>—</td><td>2.9</td><td>—</td></tr><tr><td>Oleic acid</td><td>2136</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td><td>—</td></tr><tr><td>Total</td><td>98.3</td><td>95.7</td><td>96.1</td><td>99.5</td><td>88.5</td><td>97.6</td><td>87.3</td><td>94.9</td><td>93.6</td><td>97.4</td><td>91.8</td><td>85.2</td><td></td></tr></tbody></table>

A: Agbassa; B: Bakabaka; B<sub>1</sub>; Bassila<sub>1</sub>; B<sub>2</sub>; Bassila<sub>2</sub>; NV; Natitingou Ville; Pn: Penelan; Pu: Penessoulou; TB: trunk barks; RB: root barks; L: leaves; R: roots; Kl: kovats index.
t = trace (<0.1%).
then acidified to precipitate the FA ($5 \le \text{pH} \le 6$). The FA released was yet extracted by $3 \times 50$ mL of diethyl ether [19, 20].

**FA Methylation.** Fatty acids were converted to their methylic esters by addition a methanolic solution (10%) of boron trifluoride ($BF_3$), and the methylic esters were extracted with cyclohexane.

The analysis of FA and Un collected was made through GC/FID and GC/MS [21].

## 2.4. GC/FID and GC/MS Analysis.
The essential oils were analyzed on a Hewlett-Packard gas chromatograph Model 5890, coupled with a Hewlett-Packard MS model 5871, equipped with a DB5 MS column ($30 \text{ m} \times 0.25 \text{ mm}, 0.25 \mu\text{m}$), programming from 50°C (5 min) to 300°C at 5°C/mn, 5 min hold. Helium as carrier gas (1.0 mL/min); injection in split mode (1:30); injector and detector temperature, 280 and 280°C respectively. The MS works in electron impact mode at 70 eV; electronmultiplier: 2500 eV; ion source temperature: 180°C; mass spectra data were acquired in the scan mode in *m/z* range 33–450. The essential oil was analysed on a Hewlett-Packard gas chromatograph Model 6890, equipped with a DB5 MS column ($30 \text{ m} \times 0.25 \text{ mm}, 0.25 \mu\text{m}$), programming from 50°C (5 min) to 300°C at 5°C/mn, 5 min hold. Hydrogen as carrier gas (1.0 mL/min); injection in split mode (1:60); injector and detector temperature, 280 and 300°C respectively. The essential oil is diluted in hexane: 1/30.

The compounds found by GC in the different essential oils were identified by comparing their retention indices with those of reference compounds in the literature. Their identities were further confirmed using GC/MS and comparing their mass spectra with those of reference substances [22–26].

# 3. Results and Discussion

The characterization tests showed that the mucilages, coumarins, and gallic tannins were the groups of compounds abundantly identified in the *P. butyracea* leaves (Table 1). Low levels of flavones, sterols, and triterpenes then saponins (foam index = 50.0) were also revealed in that leaves.

The fatty acids proportion, determined with regard to the mass of dry vegetable material, varied from 0.3 to 1.3%. These values were very low compared to that which has been identified in *Vetiveria zizanioides* (31.3%) by Champagnat [27]. Also, the unsaponifiable rates of the different parts of *P. butyracea* ranged between 0.7 and 1.6%. It was noted in the leaves an unsaponifiable rates similar to that reported by Dencausse (1.5%) in 1995 after extracting *P. butyracea* butter unsaponifiables [8]. The roots, leaves, and barks of *P. butyracea* were characterized by a not interesting chemical composition in fatty acids. Indeed, the proportions of fatty acids obtained were very low (<0.2%). In the leaves, it was noted the presence of fatty acids such as palmitic (0.16%), oleic (0.1%), arachidic (0.1%), and linoleic (0.1%) acids, whereas *P. butyracea* roots were only characterized by the tricosanoic acid (0.1%). In the roots, no fatty acid was identified. The unsaponifiable fractions extracted from *P.*

*butyracea* organs contained 13 to 20 compounds distributed between 55.8% and 73.0% of sterols and 10.7%–33.2% of triterpenes (Table 2).

The main sterol common to all the parts of the *P. butyracea* tree was 9,19-cyclolanost-24-en-3β-3-ol (49.3 to 72.6%) (Table 2). The taraxasterol percentages appeared in the trunk bark, the roots, and the root barks were, respectively, 30.1, 21.3 and 18.4%. The sterols identified in these organs were different from those identified by Tchobo et al. in *P. butyracea* seeds unsaponifiables. In fact, the major sterol identified in *P. butyracea* butter by Tchobo et al. was stigmasterol estimated to 68.0% [13]. This difference would denote the inhomogeneity of the chemical constituents that may arise between the various parts of a plant. The leaves were specifically constituted by the other compounds in particular friedooleanan-3-one (10.0%), phytol (8.5%), nonacosane (3.7%), hentriacontane (3.3%), and squalene (2.6%). Some terpenic compounds remarkable by their percentages were also identified in the different parts of *P. butyracea*. These were, firstly, the β-caryophyllene (4.4–7.8% in the roots) and secondly the α-copaene (4.3% in the leaves). It is necessary to indicate that the nonacosane and the hentriacontane were two hydrocarbons saturated usual of the vegetable kingdom [27], having each an odd number of carbon atoms. *P. butyracea* was one of the many non ligneous forest products which leaves, barks, and roots have an aromatic character with a producing power of essential oils. The pursuit of the chemical constituent investigations of this plant species was also made by extracting essential oils from *P. butyracea* different parts collected in several forests of the northern region of Benin. The yields of essential oils, which ranged from $50.10 \times 10^{-2}$% to $1.41 \times 10^{-2}$%, were the averages of three runs (Table 3).

The results (Table 3) showed that the essential oils from leaves, bark, and roots of *P. butyracea* have constituted mainly by hydrocarbons sesquiterpene (56.2–98.2%). Comparatively to sesquiterpene hydrocarbons, the oxygenated sesquiterpene was very poorly represented in these volatile extracts (1.2–9.5%). These volatile extracts were also low in volatile oxygenated (≤4.1%) and hydrocarbon (0.0–1.6%) monoterpenes. These sesquiterpenoids compounds predominance would result from the metabolic reactions inside the vegetable from which the precursors were farnesyl pyrophosphate isomers (2Z, 6E) and (2E, 6E) [28]. Eleven and thirty-eight compounds, representing 85.2% to 99.5% of the total weight of the essential oils, have been identified independently of the investigated portion of the shaft. The major substance present in all the essential oils samples, in amounts according to the organ origin, was α-caryophyllene (14.9–77.9%). Other major compounds were noted: α-aromadendrene (43.5%), copaene (18.4–26.6%), α-ylangene (21.1%), α-copaene (19.9%), α-humulene (6.0–13.3%), (2E, 6Z)-α-farnesene (12.6%), seychellene (12.0%), β-cubebene (6.2%), palmitic acid (10.6%), selina-3,7(11)-diene (13.3%), germacrene-B (5.1–13.5%), zonarene (9.9%), caryophyllene oxide (4.2–7.3%), β-curcumene (4.3–5.3%), δ-amorphene (4.9%), 8-cadinene (4.3%), and (E)-nerolidol (4.2%). Although all samples of *P. butyracea* studied during
the current works have been collected in the north of Benin characterized by the same climate (Sudano-Guinean), the rates in β-caryophyllene stemming from the chemical profiles obtained from analysis by GC/MS performed varied from one region to another then according to each every treated organ (Table 3). The explanation for this situation could probably denote of the influence of the soil nature of the different regions of harvest and the plant vegetative state at the date of the diverse crops. So according to the chemical profile of the *P. butyracea* essential oil targeted to NV, the rate in β-caryophyllene of roots (64.0%) is closed to that of the barks and of the roots (64.1%), but these values were slightly lower than the β-caryophyllene percentage of TB (67.4%) of the same tree. The only tree of *P. butyracea* whose leaves have produced an interesting rate of β-caryophyllene (56.4%) resulted from B₁. This value was well below the one that brought back from *P. butyracea* leaves essential oil of Natitingou (58.0%) in 2010 [12]. The lowest proportion of β-caryophyllene (14.9%) was recorded in the volatile extract of *P. butyracea* leaves harvested in Pu. It is necessary to notice that only the barks collected at B1 contained a high rate of aromadendrene (43.5%), while α-ylangene (21.1%), (2E, 6Z)-α-farnesene (12.6%), seychellene (12.0%), and palmitic acid (10.6%) really characterized the volatile extracts from leaves harvested, respectively in B₂, B₁, and A in Benin.

## 4. Conclusion

In view of the obtained results, it appears that *P. butyracea*, beyond the strong content fat of its seeds and its potential exploitation for the manufacturing of the butter was a big source (spring) of the other metabolites. The phytochemical investigations realized by using appropriate solvents showed in the *P. butyracea* leaves the presence of instrong proportion of coumarins, tannins gallic, mucilages, 9.19-cyclolanost-24-en-3β-3-ol, taraxasterol, and fatty acids tracks. The essential oil of this plant is potentially rich in sesquiterpenes hydrogenated, in particular β-caryophyllene appeared in all the parts of the vegetable species. Further investigation on a larger number of individuals collected on the explored sites and somewhere else in Benin will allow highlighting essential chemotypes at *P. butyracea* Sabine volatile extract.

## References

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[27] P. Champagnat, *Etude phytochimique sur les Poaceae: analyses de Vetiveria zizanoïdes et de Vetiveria nigritana, recherche de quelques activités biologiques* [Ph.D. thesis], Université d'Auvergne, 2007.

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