# Corrigendum: Investigating genetic diversity within the most abundant and prevalent non-pathogenic leaf-associated bacteria interacting with Arabidopsis thaliana in natural habitats

Daniela Ramírez-Sánchez, Chrystel Gibelin-Viala, Baptiste Mayjonade, Rémi Duflos, Elodie Belmonte, Vincent Pailler, Claudia Bartoli, Sébastien Carrere, Fabienne Vailleau, Fabrice Roux

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Daniela Ramírez-Sánchez, Chrystel Gibelin-Viala, Baptiste Mayjonade, Rémi Duflos, Elodie Belmonte, et al. Corrigendum: Investigating genetic diversity within the most abundant and prevalent non-pathogenic leaf-associated bacteria interacting with Arabidopsis thaliana in natural habitats. Frontiers in Microbiology, 2023, 14 (1304377), 10.3389/fmicb.2023.1304377. hal-04505635

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OPEN ACCESS

APPROVED BY
Frontiers Editorial Office,
Frontiers Media SA, Switzerland

*CORRESPONDENCE

Fabrice Roux

<figure><img src="image_2.png" /></figure>

fabrice.roux@inrae.fr

†These authors have contributed equally to this work and share first authorship

‡These authors share senior authorship

RECEIVED 29 September 2023

ACCEPTED 02 October 2023

PUBLISHED 11 October 2023

CITATION

Ramírez-Sánchez D, Gibelin-Viala C, Mayjonade B, Duflos R, Belmonte E, Pailler V, Bartoli C, Carrere S, Vailleau F and Roux F (2023) Corrigendum: Investigating genetic diversity within the most abundant and prevalent non-pathogenic leaf-associated bacteria interacting with *Arabidopsis thaliana* in natural habitats. *Front. Microbiol.* 14:1304377. doi: 10.3389/fmicb.2023.1304377

COPYRIGHT

© 2023 Ramírez-Sánchez, Gibelin-Viala, Mayjonade, Duflos, Belmonte, Pailler, Bartoli, Carrere, Vailleau and Roux. This is an open-access article distributed under the terms of the [Creative Commons Attribution License (CC BY)](https://creativecommons.org/licenses/by/4.0/). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

# Corrigendum: Investigating genetic diversity within the most abundant and prevalent non-pathogenic leaf-associated bacteria interacting with *Arabidopsis thaliana* in natural habitats

Daniela Ramírez-Sánchez¹†, Chrystel Gibelin-Viala¹†, Baptiste Mayjonade¹†, Rémi Duflos¹, Elodie Belmonte², Vincent Pailler², Claudia Bartoli³, Sébastien Carrere¹, Fabienne Vailleau¹† and Fabrice Roux¹*‡

¹LIPME, INRAE, CNRS, Université de Toulouse, Castanet-Tolosan, France, ²Gentyane, UMR 1095 GDEC, INRAE, Université Clermont Auvergne, Clermont-Ferrand, France, ³Institute for Genetics, Environment and Plant Protection (IGEPP), INRAE, Institut Agro AgroCampus Ouest, Université de Rennes 1, Le Rheu, France

KEYWORDS

microbiota, commensal bacteria, genomic diversity, plant growth promotion, growth kinetics, seed inoculation, seedling inoculation, genotype-by-genotype interactions

A corrigendum on

Investigating genetic diversity within the most abundant and prevalent non-pathogenic leaf-associated bacteria interacting with *Arabidopsis thaliana* in natural habitats

by Ramírez-Sánchez, D., Gibelin-Viala, C., Mayjonade, B., Duflos, R., Belmonte, E., Pailler, V., Bartoli, C., Carrere, S., Vailleau, F., and Roux, F. (2022). *Front. Microbiol.* 13:984832. doi: 10.3389/fmicb.2022.984832

In the published article, there was an error in the Discussion section. The wording to describe the effect of four bacterial species on plant biostimulation and biocontrol against bacterial pathogens was not precise enough and might have led to some confusion.

A correction has been made to Discussion, *Extensive genetic and genomic diversities within leaf-associated bacterial operational taxonomic units*, second paragraph. Two sentences previously stated:

"Genome sequencing confirmed or refined the *gyrB*-based taxonomic affiliation of four OTUs, i.e., *P. fungorum* (OTU2), *Methylobacterium* sp. (OTU13) and the two *Pseudomonas* species *P. moraviensis* (OTU5) and *P. siliginis* (OTU6). All these four bacterial species have been shown to act as biocontrol agents, to affect root development, to promote vegetative growth and ultimately yield, of diverse plants such as *A. thaliana*, potato, strawberry, tomato ad wheat (Hultberg et al., 2010; Ul Hassan and Bano, 2015; Rafikova et al., 2016; Klikno and Kutschera, 2017; Rahman et al., 2018; Grossi et al., 2020). In addition, both *P. moraviensis*
<figure><img src="image_2.png" /></figure>

OPEN ACCESS

APPROVED BY
Frontiers Editorial Office,
Frontiers Media SA, Switzerland

*CORRESPONDENCE

Fabrice Roux

<figure><img src="image_3.png" /></figure>

fabrice.roux@inrae.fr

†These authors have contributed equally to this work and share first authorship

‡These authors share senior authorship

RECEIVED 29 September 2023

ACCEPTED 02 October 2023

PUBLISHED 11 October 2023

CITATION

Ramírez-Sánchez D, Gibelin-Viala C, Mayjonade B, Duflos R, Belmonte E, Pailler V, Bartoli C, Carrere S, Vailleau F and Roux F (2023) Corrigendum: Investigating genetic diversity within the most abundant and prevalent non-pathogenic leaf-associated bacteria interacting with *Arabidopsis thaliana* in natural habitats. *Front. Microbiol.* 14:1304377. doi: 10.3389/fmicb.2023.1304377

COPYRIGHT

© 2023 Ramírez-Sánchez, Gibelin-Viala, Mayjonade, Duflos, Belmonte, Pailler, Bartoli, Carrere, Vailleau and Roux. This is an open-access article distributed under the terms of the [Creative Commons Attribution License (CC BY)](https://creativecommons.org/licenses/by/4.0/). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

# Corrigendum: Investigating genetic diversity within the most abundant and prevalent non-pathogenic leaf-associated bacteria interacting with *Arabidopsis thaliana* in natural habitats

Daniela Ramírez-Sánchez¹†, Chrystel Gibelin-Viala¹†, Baptiste Mayjonade¹†, Rémi Duflos¹, Elodie Belmonte², Vincent Pailler², Claudia Bartoli³, Sébastien Carrere¹, Fabienne Vailleau¹† and Fabrice Roux¹*†

¹LIPME, INRAE, CNRS, Université de Toulouse, Castanet-Tolosan, France, ²Gentyane, UMR 1095 GDEC, INRAE, Université Clermont Auvergne, Clermont-Ferrand, France, ³Institute for Genetics, Environment and Plant Protection (IGEPP), INRAE, Institut Agro AgroCampus Ouest, Université de Rennes 1, Le Rheu, France

KEYWORDS

microbiota, commensal bacteria, genomic diversity, plant growth promotion, growth kinetics, seed inoculation, seedling inoculation, genotype-by-genotype interactions

A corrigendum on

Investigating genetic diversity within the most abundant and prevalent non-pathogenic leaf-associated bacteria interacting with *Arabidopsis thaliana* in natural habitats

by Ramírez-Sánchez, D., Gibelin-Viala, C., Mayjonade, B., Duflos, R., Belmonte, E., Pailler, V., Bartoli, C., Carrere, S., Vailleau, F., and Roux, F. (2022). *Front. Microbiol.* 13:984832. doi: 10.3389/fmicb.2022.984832

In the published article, there was an error in the Discussion section. The wording to describe the effect of four bacterial species on plant biostimulation and biocontrol against bacterial pathogens was not precise enough and might have led to some confusion.

A correction has been made to Discussion, *Extensive genetic and genomic diversities within leaf-associated bacterial operational taxonomic units*, second paragraph. Two sentences previously stated:

"Genome sequencing confirmed or refined the *gyrB*-based taxonomic affiliation of four OTUs, i.e., *P. fungorum* (OTU2), *Methylobacterium* sp. (OTU13) and the two *Pseudomonas* species *P. moraviensis* (OTU5) and *P. siliginis* (OTU6). All these four bacterial species have been shown to act as biocontrol agents, to affect root development, to promote vegetative growth and ultimately yield, of diverse plants such as *A. thaliana*, potato, strawberry, tomato ad wheat (Hultberg et al., 2010; Ul Hassan and Bano, 2015; Rafikova et al., 2016; Klikno and Kutschera, 2017; Rahman et al., 2018; Grossi et al., 2020). In addition, both *P. moraviensis*
and *P. siliginis* have been identified as the main candidate bacterial species controlling most members of the root and leaf bacterial pathobiota, in particular *P. viridiflava* and *X. campestris*, across natural populations of *A. thaliana* located south-west of France (Bartoli *et al.*, 2018)."

The corrected sentences appear below:

"Genome sequencing confirmed or refined the gyrB-based taxonomic affiliation of four OTUs, i.e., *P. fungorum* (OTU2), *Methylobacterium* sp. (OTU13) and the two *Pseudomonas* species *P. moraviensis* (OTU5) and *P. siliginis* (OTU6). All these four bacterial species have been shown to act either as biocontrol agents, or to affect root development, or to promote vegetative growth and ultimately yield, of diverse plants such as *A. thaliana*, potato, strawberry, tomato and wheat (Hultberg et al., 2010; Ul Hassan and Bano, 2015; Rafikova et al., 2016; Klikno and Kutschera, 2017; Rahman et al., 2018; Grossi et al., 2020). In addition, both *P. moraviensis* and *P. siliginis* have been identified in a large

consortium of other bacterial species as candidate antagonists of the root and leaf bacterial pathobiota, in particular *P. viridiflava* and *X. campestris*, across natural populations of *A. thaliana* located south-west of France (Bartoli *et al.*, 2018)."

The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.

## Publisher's note

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.

## References

Bartoli, C., Frachon, L., Barret, M., Rigal, M., Huard-Chauveau, C., Mayjonade, B., et al. (2018). In situ relationships between microbiota and potential pathobiota in *Arabidopsis thaliana*. *ISME J.* 12, 2024–2038. doi: 10.1038/s41396-018-0152-7

Grossi, C. E. M., Fantino, E., Serral, F., Zawoznik, M. S., Fernandez Do Porto, D. A., and Ulloa, R. M. (2020). *Methylobacterium* sp. 2A is a plant growth-promoting rhizobacteria that has the potential to improve potato crop yield under adverse conditions. *Front. Plant Sci.* 11:71. doi: 10.3389/fpls.2020.00071

Hultberg, M., Alsberg, T., Khalil, S., and Alsanius, B. (2010). Suppression of disease in tomato infected by Pythium ultimum with a biosurfactant produced by *Pseudomonas* koreensis. *Biocontrol* 55, 435–444.

Klikno, J., and Kutschera, U. (2017). Regulation of root development in *Arabidopsis thaliana* by phytohormone-secreting epiphytic

methylobacteria. *Protoplasma* 254, 1867–1877. doi: 10.1007/s00709-016-1067-7

Rafikova, G. F., Korshunova, T. Y., Minnebaev, L. F., Chetverikov, S. P., and Loginov, O. N. (2016). A new bacterial strain, *Pseudomonas* koreensis IB-4, as a promising agent for plant pathogen biological control. *Microbiology* 85, 333–341.

Rahman, M., Sabir, A. A., Mukta, J. A., Khan, M. M. A., Mohi-Ud-Din, M., Miah, M. G., et al. (2018). Plant probiotic bacteria Bacillus and Paraburkholderia improve growth, yield and content of antioxidants in strawberry fruit. *Sci. Rep.* 8:2504. doi: 10.1038/s41598-018-20235-1

Ul Hassan, T., and Bano, A. (2015). The stimulatory effects of L-tryptophan and plant growth promoting rhizobacteria (PGPR) on soil health and physiology of wheat. *J. Soil Sci. Plant Nutr.* 15, 190–201.
and *P. siliginis* have been identified as the main candidate bacterial species controlling most members of the root and leaf bacterial pathobiota, in particular *P. viridiflava* and *X. campestris*, across natural populations of *A. thaliana* located south-west of France (Bartoli *et al.*, 2018)."

The corrected sentences appear below:

"Genome sequencing confirmed or refined the gyrB-based taxonomic affiliation of four OTUs, i.e., *P. fungorum* (OTU2), *Methylobacterium* sp. (OTU13) and the two *Pseudomonas* species *P. moraviensis* (OTU5) and *P. siliginis* (OTU6). All these four bacterial species have been shown to act either as biocontrol agents, or to affect root development, or to promote vegetative growth and ultimately yield, of diverse plants such as *A. thaliana*, potato, strawberry, tomato and wheat (Hultberg *et al.*, 2010; Ul Hassan and Bano, 2015; Rafikova *et al.*, 2016; Klikno and Kutschera, 2017; Rahman *et al.*, 2018; Grossi *et al.*, 2020). In addition, both *P. moraviensis* and *P. siliginis* have been identified in a large

consortium of other bacterial species as candidate antagonists of the root and leaf bacterial pathobiota, in particular *P. viridiflava* and *X. campestris*, across natural populations of *A. thaliana* located south-west of France (Bartoli *et al.*, 2018)."

The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.

## Publisher's note

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.

## References

Bartoli, C., Frachon, L., Barret, M., Rigal, M., Huard-Chauveau, C., Mayjonade, B., et al. (2018). In situ relationships between microbiota and potential pathobiota in *Arabidopsis thaliana*. *ISME J.* 12, 2024–2038. doi: 10.1038/s41396-018-0152-7

Grossi, C. E. M., Fantino, E., Serral, F., Zawoznik, M. S., Fernandez Do Porto, D. A., and Ulloa, R. M. (2020). *Methylobacterium* sp. 2A is a plant growth-promoting rhizobacteria that has the potential to improve potato crop yield under adverse conditions. *Front. Plant Sci.* 11:71. doi: 10.3389/fpls.2020.00071

Hultberg, M., Alsberg, T., Khalil, S., and Alsanius, B. (2010). Suppression of disease in tomato infected by Pythium ultimum with a biosurfactant produced by *Pseudomonas* koreensis. *Biocontrol* 55, 435–444.

Klikno, J., and Kutschera, U. (2017). Regulation of root development in *Arabidopsis thaliana* by phytohormone-secreting epiphytic

methylobacteria. *Protoplasma* 254, 1867–1877. doi: 10.1007/s00709-016-1067-7

Rafikova, G. F., Korshunova, T. Y., Minnebaev, L. F., Chetverikov, S. P., and Loginov, O. N. (2016). A new bacterial strain, *Pseudomonas* koreensis IB-4, as a promising agent for plant pathogen biological control. *Microbiology* 85, 333–341.

Rahman, M., Sabir, A. A., Mukta, J. A., Khan, M. M. A., Mohi-Ud-Din, M., Miah, M. G., et al. (2018). Plant probiotic bacteria Bacillus and Paraburkholderia improve growth, yield and content of antioxidants in strawberry fruit. *Sci. Rep.* 8:2504. doi: 10.1038/s41598-018-20235-1

Ul Hassan, T., and Bano, A. (2015). The stimulatory effects of L-tryptophan and plant growth promoting rhizobacteria (PGPR) on soil health and physiology of wheat. *J. Soil Sci. Plant Nutr.* 15, 190–201.