# Widespread Mutations in Voltage-Gated Sodium Channel Gene of Cimex lectularius (Hemiptera: Cimicidae) Populations in Paris

Mohammad Akhoundi, Dahlia Chebbah, Denis Sereno, Anthony Marteau, Julie Jan, Christiane Bruel, Nohal Elissa, Arezki Izri

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Mohammad Akhoundi, Dahlia Chebbah, Denis Sereno, Anthony Marteau, Julie Jan, et al. Widespread Mutations in Voltage-Gated Sodium Channel Gene of Cimex lectularius (Hemiptera: Cimicidae) Populations in Paris. International Journal of Environmental Research and Public Health, 2021, 18 (2), pp.407. 10.3390/ijerph. hal-03152065

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Article

# Widespread Mutations in Voltage-Gated Sodium Channel Gene of *Cimex lectularius* (Hemiptera: Cimicidae) Populations in Paris

Mohammad Akhoundi <fnref n="1" />,*, Dahlia Chebbah <fnref n="1" />,<fnref n="2" />, Denis Sereno <fnref n="3" />,<fnref n="4" />✉, Anthony Marteau <fnref n="1" />, Julie Jan <fnref n="5" />, Christiane Bruel <fnref n="5" />, Nohal Elissa <fnref n="2" /> and Arezki Izri <fnref n="1" />,<fnref n="6" />

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**Citation:** Akhoundi, M.; Chebbah, D.; Sereno, D.; Marteau, A.; Jan, J.; Bruel, C.; Elissa, N.; Izri, A. Widespread Mutations in Voltage-Gated Sodium Channel Gene of *Cimex lectularius* (Hemiptera: Cimicidae) Populations in Paris. *Int. J. Environ. Res. Public Health* **2021**, *18*, 407. https://doi.org/10.3390/ijerph18020407

Received: 17 November 2020

Accepted: 5 January 2021

Published: 6 January 2021

**Publisher's Note:** MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations.

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**Copyright:** © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

**Abstract:** Bed bugs, *Cimex lectularius* and *C. hemipterus*, are common blood-sucking ectoparasites of humans with a large geographical distribution, worldwide. In France, little is known about the status of bed bugs' infestation and their resistance to insecticides, particularly, pyrethroids. Here, we aimed to find mutations in the *kdr* gene, known to be involved in resistance to insecticides. We gathered bed bugs from various infested locations, including 17 private houses, 12 HLM building complex, 29 apartments, 2 EHPAD, and 2 immigrants' residences. A total of 1211 bed bugs were collected and morphologically identified as *C. lectularius*. Two fragments of the *kdr* gene, encompassing codons V419L and L925I, were successfully amplified for 156 specimens. We recorded sense mutation in the first amplified fragment (*kdr1*) in 89 out of 156 (57%) samples, in which in 61 out of 89 (68.5%) sequences, a change of valine (V) into leucine (L) V419L was observed. Within the second fragment (*kdr2*), a homozygous mutation was recorded in 73 out of 156 (46.7%) specimens at the codon 925. At this position, 43 out of 73 (58.9%) specimens had a sense mutation leading to the replacement of leucine (L) by isoleucine (I). Among 162 mutant sequences analyzed (89 for the *kdr1* fragment and 73 for the *kdr2* one), we detected single point mutation in 26.6%, while 73.4% presented the mutation in both *kdr1* and *kdr2* fragments. All modifications recorded in bed bug populations of Paris are described to be involved in the knockdown resistance (*kdr*) against pyrethroids.

**Keywords:** *Cimex lectularius*; insecticides; pyrethroids; voltage-gated sodium channel (VGSC); SNP

## 1. Introduction

Bed bugs, *Cimex lectularius* and *C. hemipterus*, are obligate blood-sucking insects belonging to the Cimicidae family, which feed on human blood [1,2]. Since their resurgence in the late 1990s, bed bug infestation of human habitats has drastically increased, leading to growing concerns [3–5]. This resurgence can be attributed at least in part to increased international travel and the development of insecticide resistance [6,7]. *C. lectularius* is a cosmopolitan species found commonly in temperate regions, while *C. hemipterus* is mainly present in tropical and subtropical areas [8].

Regarding the reports implying the bed bugs' involvement in harboring over 45 pathogens [9] and the competence of these insects in pathogenic agent transmission in

<footnote n="1">1 Parasitology-Mycology Department, Avicenne Hospital, AP-HP, Sorbonne Paris Nord University, 93009 Bobigny, France; dahlia.chebbah@gmail.com (D.C.); anthonymarteau@hotmail.fr (A.M.); arezki.izri@aphp.fr (A.I.)</footnote>

<footnote n="2">2 Service Parisien de Santé Environnementale, Sous-Direction de la Santé (SPSE), Mairie de Paris, 75019 Paris, France; nohal.elissa@paris.fr</footnote>

<footnote n="3">3 MIVEGEC, Institut de Recherche pour le Développement, Montpellier University, 34032 Montpellier, France; denis.sereno@ird.fr</footnote>

<footnote n="4">4 InterTryp, Institut de Recherche pour le Développement, Montpellier University, 34032 Montpellier, France</footnote>

<footnote n="5">5 Agence Régionale de Santé (ARS) Île-de-France, 35, rue de la Gare, 75935 Paris CEDEX 19, France; julie.jan@ars.fr (J.J.); christiane.bruel@ars.fr (C.B.)</footnote>

<footnote n="6">6 Unité des Virus Emergents (UVE: Aix Marseille Univ, IRD 190, INSERM 1207, IHU Méditerranée Infection), 13005 Marseille, France</footnote>

<footnote>* Correspondence: m.akhoundi@yahoo.com</footnote>
the laboratory setting [10,11], there is still no evidence certifying their vectorial role in the endemic areas [1,12]. The discovery and widespread use of organochlorine DDT (dichlorodiphenyltrichloroethane) as a powerful insecticide in 1939, has led to a drastic decline in bed bug infestations [13]. Nevertheless, insecticide resistance is currently reported, worldwide [14]. The first description of resistance to insecticides (DDT) was documented in 1947 on Hawaii Island [15,16]. Because of DDT's detrimental effects on human health and the environment, its use was banned in most Western countries since the 1970s [17]. During the 1950s and 1960s, organochlorine insecticides were replaced by organophosphates and carbamates due to their more effectiveness [18,19]. Organophosphate insecticides, such as chlorpyrifos and diazinon, together with carbamates, like propoxur, were insecticides of choice to control bed bugs. At the end of the 1970s, pyrethroid insecticides such as permethrin, cypermethrin, deltamethrin, and resmethrin were developed and considered alternatives. Unlike organochlorines or organophosphates, they are odorless with lower residual stability [14]. With the resurgence of bed bug infestation worldwide in the 1990s, a new generation of insecticides is available [20]. The extensive application of such commonly used insecticides is suspected of favoring the emergence of insecticide resistance in *C. lectularius* and *C. hemipterus* populations, worldwide. This emergence challenges the control management programs [14]. Pyrethroids are still among the most widely used insecticides against bed bugs, particularly in Europe [17,21,22].

Point mutations in the voltage-gated sodium channel (VGSC) gene reduce the target-
site sensitivity for pyrethroids and DDT, causing “knockdown resistance” (kdr) [23,24].
Three point-mutations are reported from *C. lectularius* (V419L, L925I, and I936F) and cur-
rently nine from *C. hemipterus* (L899V, M918I, D953G, Y/L995H, V1010L, I1011F, L1014F,
V1016E, and L1017F/S) [25,26]. These mutations act by substituting the amino acid
sequence of the VGSC protein that prevents the insecticide acting on the nervous sys-
tem [27,28].

Despite the wide use of chemical insecticides in France, as an essential part of control management by private pest control practitioners (PCPs) and municipalities, little is known about the bed bugs' resistance status towards pyrethroids. The first study on the resistance of bed bugs to pyrethroids was reported by Durand et al. [22] in two apartment complexes (HLM) of Saint-Ouen city in suburb of Paris. In vivo tests of insecticide susceptibility performed against bed bugs collected from suburbs of Paris concluded low susceptibility toward bendiocarb [4]. Using a molecular approach, we investigated the occurrence and frequency of mutations in the pyrethroid resistance in bed bug specimens, gathered from various Paris areas.

**2. Materials and Methods**

2.1. *Bed Bug Collections*

Bed bugs were collected within private houses, apartments, HLM building complex, EHPAD (nursing home for the elders), and immigrants' residences in Paris and surrounding cities. All inhabitants were questioned. The information on the date and history of the infestation and the possible infestation route, the history of treatment, and the chemical application were recorded individually. According to infestation signs, observed during the visual inspection, an infestation scale ranging from 0 (no infestation) to 5 (high level of infestation) was used to categorize the infested locations (Table 1).

Bed bugs were sampled using a handheld vacuum cleaner (Dyson V7 trigger) or by
entomological forceps. To avoid excessive mortality during bed bugs' collection, they were
placed into 5 mL sterile mini-glass vials with a piece of folded bound paper, simulating
an artificial shelter. Live bed bugs were brought to the laboratory and identified under a
stereomicroscope (Olympus SZ61, Japan). The identification of bed bugs was performed
based on the identification keys, published by Usinger (1966) [8] and Walpole (1987) [29].
All specimens were individually labeled and kept at -20 °C, for further molecular analysis.
<table><caption>**Table 1.** Proposed infestation scale based on the signs observed by visual inspection.</caption><thead><tr><th>Infestation Scale</th><th>Bed Bug Bite</th><th>Presence of Bed Bugs</th><th>Black Spot (Excretion)</th></tr></thead><tbody><tr><td>0</td><td>No bite on residents</td><td>No bed bugs or different nymphal stages</td><td>No black spot or bug excrement on the bed, the mattress, and the area around the bed, on the bedding, and sheets</td></tr><tr><td>1</td><td>Bites on residents</td><td>Black spot or bed bug excretion on the bed</td><td>Presence of adults, eggs, and first or second nymphal stages</td></tr><tr><td>2</td><td>Bites on residents</td><td>Numerous black spots or bed bug excretion on the bed, mattress, and the area around the bed, on bedding and sheets, or the places the resident rests</td><td>Presence of adults, eggs, and various nymphal stages</td></tr><tr><td>3</td><td>Bites on residents</td><td>Numerous black spots or bed bug excretion on the bed, in other parts of the house</td><td>Presence of adults, eggs, and different nymphal stages, bed bugs are visible during the day</td></tr><tr><td>4</td><td>Bites on residents</td><td>Numerous black spots or bed bug excretion on the bed, in other parts of the house</td><td>Presence of different nymphal stages everywhere with high numbers, bed bugs are visible during the day</td></tr><tr><td>5</td><td>Bites on residents</td><td>Numerous black spots or bed bug excretion on the bed, in other parts of the house</td><td>Presence of different nymphal stages everywhere with high numbers, bed bugs are visible during the day, several bed bugs niches visible everywhere</td></tr></tbody></table>

## 2.2. Molecular Genotyping of the kdr Gene

The extraction of DNA was carried out using Chelex 10% (Bio-Rad, California, USA) [30]. The DNA concentration was then quantified by Qubit (Thermo Scientific, Waltham, USA). The amplification of two fragments of 474 and 744 bp within the ORF of the voltage-sensitive sodium channel gene (kdr-like gene) was carried out by conventional PCR. The reaction was performed in 25 µL of reaction mixture containing 0.3 µmol/L of *kdr-1f* (fwd): (5'-AACCTGGATATACATGCCTTCAAGG-3') and *kdr-1r* (rev): (5'-TGATGGAGATTTTGCCACTGATG-3') for amplification of first fragment and *kdr-2f* (fwd): (5'-GGAATTGAAGCTGCCATGAAGTTG3-3') and *kdr-2r* (rev): (5'-TGCCTATTCTG TCGAAAGCCTCAG-3') for amplification of second fragment, 200 µmol/L dNTPs, buffer (50 mmol/L KCl, 10 mmol/L Tris-HCl, pH 8.3, and 1 mmol/L MgCl2), and 2.5 U of *Thermus aquaticus* DNA polymerase (AmpliTaq Gold; Applied Biosystems, Foster City, California, USA). The samples were incubated at 95 °C for 10 min for denaturation, followed by 40 cycles at 94 °C for 40 s, 52 °C (first fragment)/55 °C (second fragment) for 40 s, and 72 °C for 40 s. The final extension was at 72 °C for 10 min. Negative and positive controls were used for each batch of PCR. Amplicons were analyzed using electrophoresis on a 1.5% agarose gel containing ethidium bromide. All amplified fragments were subjected to bilateral DNA sequencing. The sequences were aligned against their wild-type homologous sequences (GenBank accession numbers, GU123927 and GU123928) using Basic Local Alignment Search Tool (BLAST) (www.ncbi.nlm.nih.gov/BLAST). The presence of single nucleotide polymorphism (SNP), V419K and L925I, in two amplified fragments of the *kdr* gene (*kdr1* and *kdr2*) was searched in both forward and reverse sequences using BioEdit v7.0.0 software [31].

## 2.3. Ethics Approval

This study was conducted in accordance with the Declaration of Helsinki, and the protocol was approved by the Ethics Committee of Avicenne Hospital, France (Project identification code: 95/99/AVC/ESA).
### 3. Results

This study was carried out from January to June 2019, in collaboration with the public health department of Paris municipality, providing a preliminary list of potential infested locations in various geographic areas of Paris (see Figure 1). In total, 17 private houses, 29 apartments, 12 HLM building complex, 2 EHPAD, and 2 immigrants' residences located in the 15 arrondissements of Paris (1,2,8,9,10,11,12,13,14,15,16,17,18,19 and 20) and 18 suburb cities (including Aubervilliers, Bobigny, Drancy, La Courneuve, Montreuil, Neuilly sur Marne, Pantin, and Stains (Seine-Saint-Denis department); Arcueil, Creteil, Nogent-sur-Marne, and Vincennes (Val-de-Marne department); Asnieres, Meudon, and Nanterre (Hauts-de-Seine department), Chilly Mazarin (Essonne department), Marly-le-Roi (Yvelines department), and Sarcelles (Val-d'Oise department)) were examined for the presence of bed bugs (Figure 1). Among them, 56 locations were infested by bed bugs, while in 6 locations, no bed bugs were noticed during inspection. Detail of bed bug specimens collected from different districts of Paris is given in Table 2. The number of insects collected varied, according to location ranging from one to more than 50 samples. Based on the scale of infestation level, most of the visited sites had the infestation of level 2 (22 locations) followed by level 3 (15 locations) (Table 1). A total of 1211 bed bugs belonging to various life stages (egg, nymph, adult male and female, unfed, and blood-fed) were collected. All adult specimens were morphologically identified as *C. lectularius*. The amplification of two fragments (encompassing codons 419 and 925) belonging to the VGSC gene was successfully carried out for 156 specimens, representing the geographical locations sampled. The alignment of the first amplified fragment (*kdr1*) with reference sequences collected in GenBank displayed the presence of a homozygous mutation at the codon 419 in 89 out of 156 (57%) specimens. Among these mutations, 61 out of 89 (68.5%) sequences revealed a change of valine (V) to leucine (L) V419L, while in the 28 (31.5%) remaining specimens, silent mutations were detected (Figure 2). Analysis of the second amplified fragment (*kdr2*) displayed the homozygous mutations at the codon 925 in 73 out of 156 (46.7%) specimens, in which 43 out of 73 (58.9%) specimens had a sense mutation, leading to the replacement of leucine (L) by isoleucine (I). In the remaining 30 (41.1%) specimens, mutations detected were silent (Figure 2). Among 162 mutant sequences analyzed (89 for the *kdr1* fragment and 73 for the *kdr2* one), a single mutation in one fragment was detected in 26.6% of cases. In contrast, 73.4% presented a single mutation in both amplified *kdr1* and *kdr2* fragments.

<table><caption>**Table 2.** Details of bed bug infestations in various locations observed by visual inspection in Paris and suburb cities.</caption><thead><tr><th rowspan="2">Location Type</th><th colspan="2">Number of Inspected Locations</th><th colspan="6">Number and Level of Infested Locations</th><th rowspan="2">Number of Collected Specimens</th></tr><tr><th>Paris Arrondissements</th><th>Suburb Cities</th><th>0</th><th>1</th><th>2</th><th>3</th><th>4</th><th>5</th></tr></thead><tbody><tr><td>Private house</td><td>6</td><td>11</td><td>2</td><td>3</td><td>5</td><td>5</td><td>2</td><td>0</td><td>132</td></tr><tr><td>Apartment</td><td>11</td><td>18</td><td>3</td><td>5</td><td>9</td><td>7</td><td>3</td><td>2</td><td>195</td></tr><tr><td>HLM building complex</td><td>4</td><td>8</td><td>1</td><td>3</td><td>5</td><td>2</td><td>0</td><td>1</td><td>478</td></tr><tr><td>Migrant residence</td><td>0</td><td>2</td><td>0</td><td>0</td><td>1</td><td>1</td><td>0</td><td>0</td><td>389</td></tr><tr><td>EHPAD</td><td>1</td><td>1</td><td>0</td><td>0</td><td>2</td><td>0</td><td>0</td><td>0</td><td>17</td></tr><tr><td>Total</td><td>22</td><td>40</td><td>6</td><td></td><td></td><td>56</td><td></td><td></td><td>1211</td></tr></tbody></table>

EHPAD: nursing home for the elders.
<figure><img src="image_5.png" /><figcaption>**Figure 1.** Geographical topology of the processed locations for bed bug sampling in Ile-de-France, France. 1. Paris arrondissements, 2: Seine-Saint-Denis, 3: Val-de-Marne, 4: Hauts-de-Seine, 5: Seine-et-Marne, 6: Essonne, 7: Yvelines, and 8: Val-d'Oise.</figcaption></figure>

<figure><img src="image_6.png" /><figcaption>**Figure 2.** DNA sequence alignment of the voltage-gated sodium channel (VGSC) gene fragment encompassing nucleotides corresponding to the codons V419L (Panel A) and L925I (Panel B) in various *C. lectularius* specimens collected from Paris and suburb cities.</figcaption></figure>
## 4. Discussion

With 64 million tourists in 2019, Paris belongs to the most visited city in the world. Therefore, bed bug infestations' control remains a significant challenge in public health [32], with physical and psychological issues [33,34]. Despite increasing concerns reported by pest control practitioners (PCPs) and municipalities in the recent decade, no official report on the rate of bed bug infestation and control management success with chemical insecticides is available. Herein, we performed a survey of mutations occurring in the VGSC gene of *C. lectularius* populations collected from 15 out of the 20 "arrondissements" of Paris and 18 suburb cities. Our survey highlighted a high prevalence of bed bug infestation (56 out of 62 processed locations infested), with most infested sites being scale 2 (22/56, 39.2%). These findings are in accordance with two previous investigations carried out in the suburbs of Paris [4,22]. Furthermore, we did not find any correlation between the infestation levels defined in Table 1 and *kdr* point mutations in the processed bed bug populations. Based on epidemiological information gathered from inhabitants of the infested locations during inspections, 27.4% and 21.3% stated second-hand materials and infested objects (particularly travel suitcase), respectively, as the possible way of bed bug infestation, respectively, and 51.3% had no idea about the infestation source.

Since the introduction of synthetic insecticides, selection and adaptation of bed bugs might have occurred, allowing them to survive [35]. Among described mechanisms, target-site mutation and metabolic resistance are generally thought to be responsible for insecticide resistance in bed bugs [14]. The determination of bed bugs' resistance status is usually performed by in vivo contact bioassays [4] or via identification of SNPs of target genes, known to be involved in metabolic resistance [22]. The VGSC expressed in the insect's nervous system, is a target gene for which molecular markers of resistance to pyrethroids are described [20,36,37]. Bed bugs resistant to these insecticides display the point mutations in the VGSC gene. The presence of these SNPs correlates to the resistance in bed bugs against pyrethroids [20,36,37]. The L925I mutation in the *kdr* gene appears to be positively selected, more frequently than the V419L mutation, for pyrethroid resistance [36]. We reported a *kdr* gene haplotype with homozygous mutation of L925I and homozygous wild-type V419 codon, found in 61/89 (68.5%) and 43/73 (58.9%) of bed bugs collected in Paris and suburb cities. Strikingly a large majority (73.4%) of bed bugs collected in Paris bear both V419L and L925I mutations. To what extend these mutations impact the level of pyrethroid resistance needs further investigations. In particular, to firmly confirm bed bug resistance, further analysis using in vivo bioassays is required. These would shed light on the level of resistance to pyrethroid with single L925I or V419L mutations, compared to the combined effect of two mutations in specimens.

## 5. Conclusions

Our results highlight the predominance of pyrethroids resistance mutations in all populations collected. These would consequently affect these chemicals' ineffectiveness in the control of bed bugs in Paris and suburb cities. These observations would prompt to reevaluate the intensive use of pyrethroids to control the bed bug infestations in Paris. The replacement of chemical treatments by nonchemical alternatives (such as dry heating or freezing) or the development of new eco-friendly alternative insecticides can reduce these insecticides' harmful impacts.

**Author Contributions:** Conceptualization, M.A. and A.I.; methodology, M.A., D.C., D.S., A.M., J.J., C.B., N.E., and A.I.; formal analysis, M.A., D.C., D.S., and A.I.; investigation, M.A., D.C., D.S., A.M., J.J., C.B., N.E., and A.I.; validation, M.A., D.S., and A.I.; data curation, M.A., D.C., D.S., J.J., C.B., N.E., and A.I.; writing—original draft preparation, M.A. and A.I.; writing—review and editing, D.S., N.E., and A.I.; visualization, M.A. and A.I.; supervision, M.A. and A.I. All authors have read and agreed to the published version of the manuscript.

**Funding:** The authors are grateful to the district authority of Stains, Plaine commune of Stains and Seine-Saint-Denis habitat for their valuable contributions in the bed bugs sampling. This study was
partly carried out within the framework of a CIFRE grant co-financed by the Association Nationale pour la Recherche et la Technologie and the City of Paris.

**Institutional Review Board Statement:** The study was conducted according to the guidelines of the Declaration of Helsinki, and approved by the Ethics Committee of Avicenne Hospital, France (Project identification code: 95/99/AVC/ESA).

**Informed Consent Statement:** Not applicable.

**Data Availability Statement:** Data sharing not applicable.

**Conflicts of Interest:** The authors declare no conflict of interest.

## References

1. Akhoundi, M.; Sereno, D.; Durand, R.; Mirzaei, A.; Bruel, C.; Delaunay, P.; Marty, P.; Izri, A. Bed Bugs (Hemiptera, Cimicidae): Overview of Classification, Evolution and Dispersion. *Int. J. Environ. Res. Public Health* **2020**, *17*, 4576. [CrossRef]

2. Akhoundi, M.; Sereno, D.; Marteau, A.; Bruel, C.; Izri, A. Who Bites Me? A Tentative Discriminative Key to Diagnose Hematophagous Ectoparasites Biting Using Clinical Manifestations. *Diagnostics (Basel)* **2020**, *10*, 308. [CrossRef]

3. Akhoundi, M.; Kengne, P.; Cannet, A.; Brengues, C.; Berenger, J.M.; Izri, A.; Marty, P.; Simard, F.; Fontenille, D.; Delaunay, P. Spatial genetic structure and restricted gene flow in bed bugs (*Cimex lectularius*) populations in France. *Infect. Genet. Evol.* **2015**, *34*, 236–243. [CrossRef]

4. Candy, K.; Akhoundi, M.; Bruel, C.; Izri, A. Ineffectiveness of Insecticide Bendiocarb Against a *Cimex lectularius* (Hemiptera: Cimicidae) Population in Paris, France. *J. Med. Entomol.* **2018**, *55*, 1648–1650. [CrossRef]

5. Parola, P.; Izri, A. Bed bugs. *N. Engl. J. Med.* **2020**, *382*, 2230–2237. [CrossRef] [PubMed]

6. Reinhardt, K.; Siva-Jothy, M.T. Biology of the bed bugs (Cimicidae). *Ann. Rev. Entomol.* **2007**, *52*, 351–374. [CrossRef] [PubMed]

7. Doggett, S.L.; Russell, R. Bed bugs. What the GP needs to know. *Aust. Fam. Physician* **2009**, *38*, 880–884. [PubMed]

8. Usinger, R.L. *Monograph of Cimicidae*; Entomological Society of America: Washington, DC, USA, 1966.

9. Delaunay, P.; Blanc, V.; Del Giudice, P.; Levy-Bencheton, A.; Chosidow, O.; Marty, P.; Brouqui, P. Bedbugs and infectious diseases. *Clin. Infect. Dis.* **2011**, *52*, 200–210. [CrossRef] [PubMed]

10. Salazar, R.; Castillo-Neyra, R.; Tustin, A.W.; Borrini-Mayorí, K.; Náquira, C.; Levy, M.Z. Bed bugs (*Cimex lectularius*) as vectors of Trypanosoma cruzi. *Am. J. Trop. Med. Hyg.* **2015**, *92*, 331–335. [CrossRef] [PubMed]

11. Leulmi, H.; Bitam, I.; Berenger, J.M.; Lepidi, H.; Rolain, J.M.; Almeras, L.; Raoult, D.; Parola, P. Competence of *Cimex lectularius* Bed Bugs for the Transmission of Bartonella quintana, the Agent of Trench Fever. *PLoS Negl. Trop. Dis.* **2015**, *9*, e0003871.

12. Doggett, S.L.; Miller, D.M.; Lee, C.Y. *Advances in the Biology and Management of Modern Bed Bugs*; Wiley: Hoboken, NJ, USA, 2017.

13. Naylor, R.; Balvín, O.; Delaunay, P.; Akhoundi, M. The bed bug resurgence in Europe and Russia. In *Advances in the Biology and Management of Modern Bed Bugs*; Doggett, S.L., Miller, D.M., Lee, C.-Y., Eds.; John Wiley & Sons: Oxford, UK, 2018; pp. 59–65.

14. Dang, K.; Doggett, S.L.; Veera Singham, G.; Lee, C.Y. Insecticide resistance and resistance mechanisms in bed bugs, *Cimex* spp. (Hemiptera: Cimicidae). *Parasites Vectors* **2017**, *10*, 318. [CrossRef] [PubMed]

15. Johnson, M.S.; Hill, A.J. Partial resistance of a strain of bedbug to DDT residual. *Med. News Lett.* **1948**, *12*, 26–28.

16. Brown, A.W.A. Insecticide resistance in mosquitoes: A pragmatic review. *J. Am. Mosq. Control Assoc.* **1986**, *2*, 123–140. [PubMed]

17. Boase, C.S.G.; Naylor, R. Interim report on insecticide susceptibility status of UK bedbugs. *Prof. Pest Controll.* **2006**, *8*, 12–13.

18. Yoon, K.S.; Kwon, D.H.; Strycharz, J.P.; Hollingsworth, C.S.; Lee, S.H.; Clark, J.M. Biochemical and molecular analysis of deltamethrin resistance in the common bed bug (Hemiptera: Cimicidae). *J. Med. Entomol.* **2008**, *45*, 1092–1101. [CrossRef]

19. Krieger, R. (Ed.) *Hayes' Handbook of Pesticide Toxicology*; Academic Press: San Diego, CA, USA, 2010; p. 2342.

20. Wood, T.J.; Goulson, D. The environmental risks of neonicotinoid pesticides: A review of the evidence post 2013. *Environ. Sci. Pollut. Res.* **2017**, *24*, 17285–17325. [CrossRef]

21. Kilpinen, O.; Kristensen, M.; Vagnjensen, K.M. Resistance differences between chlorpyrifos and synthetic pyrethroids in *Cimex lectularius* population from Denmark. *Parasitol. Res.* **2011**, *109*, 1461–1464. [CrossRef]

22. Durand, R.; Cannet, A.; Berdjane, Z.; Bruel, C.; Haouchine, D.; Delaunay, P.; Izri, A. Infestation by pyrethroids resistant bed bugs in the suburb of Paris, France. *Parasite* **2012**, *19*, 381–387. [CrossRef]

23. Raab, R.W.; Moore, J.E.; Vargo, E.L.; Rose, L.; Raab, J.; Culbreth, M.; Burzumato, G.; Koyee, A.; McCarthy, B.; Raffaele, J.; et al. New Introductions, Spread of Existing Matrilines, and High Rates of Pyrethroid Resistance Result in Chronic Infestations of Bed Bugs (*Cimex lectularius* L.) in Lower-Income Housing. *PLoS ONE* **2016**, *11*, e0117805. [CrossRef]

24. Lewis, C.D.; Levine, B.A.; Vargo, E.L.; Schal, C.; Booth, W. Recent Detection of Multiple Populations of the Tropical Bed Bug (Hemiptera: Cimicidae) Exhibiting kdr-Associated Mutations in Hawaii. *J. Med. Entomol.* **2020**, *57*, 1077–1081. [CrossRef]

25. Dang, K.; Toi, C.S.; Lilly, D.G.; Bu, W.; Doggett, S.L. Detection of knockdown resistance mutations in the common bed bug, *Cimex lectularius* (Hemiptera: Cimicidae), in Australia. *Pest Manag. Sci.* **2015**, *71*, 914–922. [CrossRef] [PubMed]

26. Punchihewa, R.; de Silva, W.P.P.; Weeraratne, T.C.; Karunaratne, S.P. Insecticide resistance mechanisms with novel 'kdr' type gene mutations in the tropical bed bug *Cimex hemipterus*. *Parasites Vectors* **2019**, *12*, 310. [CrossRef] [PubMed]
27. Dang, K.; Toi, C.S.; Lilly, D.G.; Lee, C.Y.; Naylor, R.; Tawatsin, A.; Thavara, U.; Bu, W.; Doggett, S.L. Identification of putative *kdr* mutations in the tropical bed bug, *Cimex hemipterus* (Hemiptera: Cimicidae). *Pest Manag. Sci.* **2015**, *71*, 1015–1020. [CrossRef] [PubMed]

28. Holleman, J.G.; Robison, G.A.; Bellovich, I.J.; Booth, W. Knockdown Resistance-Associated Mutations Dominate Populations of the Common Bed Bug (Hemiptera: Cimicidae) Across the South Central United States. *J. Med. Entomol.* **2019**, *56*, 1678–1683. [CrossRef]

29. Walpole, D. External morphology of the legs of two species of bed bugs (Hemiptera: Cimicidae). *J. Entomol. Soc. S. Afr.* **1987**, *50*, 193–201.

30. Walsh, P.S.; Metzger, D.A.; Higuchi, R. Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. *BioTechniques* **1991**, *10*, 506–513. [CrossRef]

31. Hall, T.A. Bioedit: A user-friendly biological sequence alignment editor and analysis program for windows 95/98/NT. *Nucleic Acids Symp. Ser.* **1999**, *41*, 95–98.

32. Jourdain, F.; Delaunay, P.; Bérenger, J.M.; Perrin, Y.; Robert, V. The Common bed bug (Cimex lectularius) in metropolitan France. Survey on the attitudes and practices of private- and public-sector professionals. *Parasite* **2016**, *23*, 38. [CrossRef]

33. Goddard, J.; deShazo, R. Bed bugs (*Cimex lectularius*) and clinical consequences of their bites. *JAMA* **2009**, *301*, 1358–1366. [CrossRef]

34. Izri, A.; Marteau, A.; Ferreira, T.; Bruel, C.; Benainous, R.; Dhote, R.; Akhoundi, M. Severe anemia due to bed bugs hyperinfestation. *Microb. Pathog.* **2020**, *149*, 104564. [CrossRef]

35. Georghiou, G.P. The evolution of resistance to pesticides. *Annu. Rev. Ecol. Syst.* **1972**, *38*, 133–168. [CrossRef]

36. Seong, K.M.; Lee, D.Y.; Yoon, K.S.; Kwon, D.H.; Kim, H.C.; Klein, T.A.; Clark, J.M.; Lee, S.H. Establishment of quantitative sequencing and filter contact vial bioassay for monitoring pyrethroid resistance in the common bed bug, *Cimex lectularius*. *J. Med. Entomol.* **2010**, *47*, 592–599. [CrossRef] [PubMed]

37. Zhu, F.; Wigginton, J.; Romero, A.; Moore, A.; Ferguson, K.; Palli, R.; Potter, M.F.; Haynes, K.F.; Palli, S.R. Widespread distribution of knockdown resistance mutations in the bed bug, *Cimex lectularius* (Hemiptera: Cimicidae), populations in the United States. *Arch. Insect Bioch. Physiol.* **2010**, *73*, 245–257. [CrossRef] [PubMed]