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# Innovative Approaches: Phyto-Probiotics as a Therapeutic Support for Managing Obesity-Linked Psoriasis

Arthur Chervet, Rawan Nehme, Clémence Defois-Fraysse, Caroline Decombat, Bertrand Evrard, Edith Filaire, Jean-Yves Berthon, Assia Dreux-Zigha, Laetitia Delort, Florence Caldefie-Chezet

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## ► To cite this version:

Arthur Chervet, Rawan Nehme, Clémence Defois-Fraysse, Caroline Decombat, Bertrand Evrard, et al.. Innovative Approaches: Phyto-Probiotics as a Therapeutic Support for Managing Obesity-Linked Psoriasis. 46th ESPEN Congress on Clinical Nutrition and Metabolism, Sep 2024, Milan (Italie), France. **hal-04875947**

HAL Id: hal-04875947

https://hal.science/hal-04875947v1

Submitted on 13 Jan 2025

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Distributed under a Creative Commons Attribution 4.0 International License
# Innovative Approaches: Phyto-Probiotics as a Therapeutic Support for Managing Obesity-Linked Psoriasis

A. Chervet¹, R. Nehme¹, C. Defois-Fraysse², C. Decombat¹, B. Evrard¹, E. Filaire¹, J.-Y. Berthon³, A. Dreux-Zigha², L. Delort¹, A. F. Caldefie-Chezet¹

¹INRAE, UNH, Unité de Nutrition Humaine, Université Clermont-Auvergne, Clermont-Ferrand, ²Greencell, Saint-Beauzire, France, ³Greentech SA, Saint-Beauzire, France

## Background

Obesity, which has become a global public health issue, is defined by an excessive accumulation of body fat. It is a chronic disease and a major risk factor for many other pathologies development such as psoriasis. Obesity epidemiology demonstrates that its prevalence has gradually increased over the last few decades. According to WHO, more than 890 million persons are estimated to be obese in 2022.

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## Objective

The aim of the project is to develop new natural health products for nutraceuticals and dermo-cosmetics. The extracts are obtained from the fermentation of a prebiotic and a probiotic. 37 combinations have been developed and investigated. Two of them have been retained for additional studies, including Phyto-Probiotics 31.

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## Targets

Low-grade Inflammation

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Obesity

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Psoriasis

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## Methods & Results

<figure><img src="image_10.png" /><figcaption>### THP-1 polarisation into M0-like or M1-like macrophages</figcaption></figure>

<figure><img src="image_11.png" /><figcaption>### Psoriatic keratinocytes</figcaption></figure>

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Reduced the gene expression of M1-like markers (TNF-α, IL-6, IL-8, IL-1β & CXCL10)

S100A7 (psoriasin): bacteriocin up-regulated in patients with psoriasis

<figure><img src="image_16.png" /><figcaption>### Co-culture mature obese adipospheroids / psoriatic keratinocytes</figcaption></figure>

In order to mimic the influence of the obese adipose microenvironment on psoriasis, mature adipospheroids (BMI>31) were co-cultured with psoriatic keratinocytes from biopsies. The adipospheroids were grown and differentiated for 10 days before being co-cultured with psoriatic keratinocytes. On the day of co-culture, the extract was applied for 24 hours.

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Decreased the gene expression of IL-6, IL-8, IL-1β, S100A7 in Psoriatic keratinocytes. Downregulated IL-6, IL-1β in adipospheroids

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Decreased the secretion of IL-6, IL-8, IL-1β, S100A7

## Conclusion

PP31 extract may exhibit a triple effect by targeting obesity, inflammation, and psoriasis biomarkers, and may help in both local and systemic management of obesity-linked psoriasis.