to be purchased at the farmers market. Factors influencing food choice for the home were: cost, taste and, as less important, nutritional quality. Participants indicated that they did not mind traveling farther distances to find better prices. Participants noted that they did not plan and prepare food according to the food they have at home. In relationship to attending workshops at Primary Care Centres, participants reported that they do not participate, but stated that their choices may be influenced if they attended.

**Conclusions:** The factors that influence food-purchasing decisions for the home of families with preschool and school age children were: cost of food and taste. It is necessary to strengthen strategies of food education that involve the nuclear family.

**Keywords:** Purchasing decision, focus group, food availability.

**Further collaborators:**

All collaborators in the abstract

144/2380

BELIEFS ABOUT THE BENEFITS AND DANGERS
OF COW'S MILK CONSUMPTION BY WOMEN
AND CHILDREN IN SOME PERI-URBAN COMMU-
NITIES IN GHANA

(1)Otoo, Gloria; (2)Colecraft, Esi; (3)Amissah, Niilante; (4)Blay-Adjei, Maame; (5)Hanson-Kunadu, Angela; (6)Afrifa-Anane, Ernest.

(1)PhD. Nutritionist. Department of Nutrition and Food Science.
University of Ghana. Ghana; (2)PhD. Nutritionist. Department of
Nutrition and Food Science. University of Ghana. Ghana.; (3)PhD.
Food Scientist. Department of Family and Consumer Sciences.
University of Ghana. Ghana.; (4)PhD. Food Scientist. Department
of Nutrition and Food Science. University of Ghana. Ghana.;

(5)PhD. Food Scientist. Department of Nutrition and Food
Science. University of Ghana. Ghana.; (6)PhD. Research Associate.
Regional Institute for Population Studies. University of Ghana.
Ghana.

**Background and objectives:** Dairy products are a source of quality protein and bioavailable micronutrients important for women of reproductive age (WRA) and children. However, negative beliefs about milk may limit their consumption. Understanding beliefs about milk relative to WRA and young children may inform interventions to enhance milk consumption and contribute to addressing the high burden of malnutrition among WRA and preschool age children. We assessed perceptions about the benefits and harms of milk consumption by WRA and young children among adults in some peri-urban communities in Ghana.

**Methods:** Exploratory qualitative study comprising 26 focus group discussions (FGD) conducted among the following groups: Adult men and different categories of WRA; mothers with children <5y, lactating mothers, pregnant women, non-pregnant and non-lactating women. Trained moderators facilitated the FGD either in English or the local language of choice. A socio-demographic questionnaire was administered and a semi-structured interview guide was used to solicit information on their beliefs and attitudes towards milk consumption. The discussions were recorded,

translated and transcribed into English. The transcripts were
then coded and analyzed using the thematic analysis approach.

**Results:** A total of 190 people participated in the FGD, 81.6% were women, 17.9% were 40y and above, 7.9% had no formal education, 14.9% were unemployed and 75.1% were married. While dairy were generally perceived as nutritious and healthy for children and WRA, the FGD also identified positive and negative beliefs about milk consumption. The most salient positive beliefs about milk consumption emphasized their perceived benefits for pregnant and lactating women. For pregnant women, milk was said to 'give blood', prevent dizziness and heartburn. For breastfeeding women, it was believed to enhance breast milk output. For young children, dairy consumption was believed to promote growth, intelligence and advance puberty. However, it was cautioned that consumption levels for milk needed to be moderated as high consumption frequency could cause diarrhea and stomach sores particularly among children.

**Conclusions:** The largely positive beliefs about milk suggest a favorable attitude towards drinking milk in the communities studied. However many of these positive and negative beliefs were misconceptions that will need to be addressed through nutrition education.

**Keywords:** Dairy, milk consumption, beliefs, benefits, Ghana

**Further collaborators:**

Sponsored by the Bill & Melinda Gates Foundation and DFID.

144/2405

INVOLVEMENT OF SOCIAL AND GENDER AS-
PECTS IN THE CHOICE OF NUTRITION

(1)Castro Alija, Mª José; (2)Jiménez Pérez, José María; (3)García del Rio, Ana; (4)Lopez Vallecillo, Maria; (4)Frutos Martin, Manuel; (2)Cao Torija, Mª José.

(1)MD, PhD. Faculty of Nursing. University of Valladolid.
Endocrinology and Clinical Nutrition Research Center (ECNRC),
University of Valladolid. Spain.; (2)RN. PhD. Nursing Faculty,
University of Valladolid. Spain. Endocrinology and Clinical
Nutrition Research Center (ECNRC), University of Valladolid.
Spain.; (3)MSNc. Nursing Faculty, University of Valladolid. Spain.;
(4)RN. PhD. Nursing Faculty, University of Valladolid. Spain.

**Background and objectives:** There are few studies about socio-cultural aspects which origin are in some food behaviour, in this particular case of women. Future studies will be required to introduce the routine demanded by dietary advice in every day life.

Determine the causes that lead to women to decide their diet.

**Methods:** Transversal descriptive study consisted of 38,455 women, of which we selected by random sampling a proportional and representative sample of 500 women, aged between 40 and 60 years. These women are subjected to an own design questionnaire, with socioeconomic, demographic variables, and some of them related with attitutes and lifestyles. We took aspects of the image body shape questionnaire of Cooper et al., (1987) and eat-
ing attitudes test of Garner et al., (1982), which we added others of interests for us.

**Results:** Firstly we consider of the women:

* Economy 45.53%

* Children and/or couple preferences 14.35%

* Fashion style 9%

* Ease of study preparation 9%

* Health 4.46%

* Other reasons (esthetic among them) 26.66%

98.19 % of women are responsible for family diet.

80.4 % of women recognizes that they eat things that are not in their interest, even though they know that it is so, conditioned by the above issues.

93.69% of women of the sample consider of interest to receive in diet topics.

**Conclusions:** Family economy is the first reason that determines the choice. Nowadays, related with the economic crisis.

About the other reasons, the main is pleasing children and couple. Fullfilling the women role to assume nutrition responsibility, that exclusively falls on them. Meaning satisfaction of all, although that may condition that the feeding of the women themselves distract from what is convenient, with consequences of their health.

**Keywords:** Gender Identity, diet, Social Determinants of Health.

**Further collaborators:**

Mª José Castro Alija (MD, PhD)<fnref n="1" />,<fnref n="2" />, Mª José Cao Torija (RN, PhD)<fnref n="1" />,<fnref n="2" />, Ana García del Río (MSc)<fnref n="1" />, María Lopez Vallecillo (RN, PhD)<fnref n="1" />, Manuel Frutos Martín (RN, PhD)<fnref n="1" />, José Mª Jiménez Pérez (RN, PhD)<fnref n="1" />,<fnref n="2" />.

**Methods:** This is an action-intervention study that belongs to na extension Project held in the year 2015. In a period of thirty days, there was the weighing of the unconventional parts of the food discarded by the institution. In the second stage, the illustrative booklet with recipes was elaborated according to the non-use of the vegetable/fruits observed. The kitchen employees also completed a self-administered questionnaire with seven mixed questions adapted from Da Rosa (2009). The questions were to evaluate the knowledge about the integral use of food and were applied before and after the course. Two practical mini-course (4 hours each) approaching the benefits of integral use of food and the preparation of 4 recipes contained in the booklet and randomly selected were made: salted pie made with peel and stalks of vegetables and sweet made with the shell / crust of watermelon with coconut; and banana peel jelly and carrot dip.

**Results:** It can be observed that the vegetables/fruits were not fully utilized by the institution. In addition, 6,672 kg of lettuce (leaf, stem), 1.278 kg of tomato, 492 g of cabbage, 678 g of arugula, 400 g of chard, 396 g broccoli were discarded for lack of storage and excessive withdrawal during pre-preparation. The results of the questionnaire showed that the employees already had some knowledge about the integral use of the food, but they didn't use the technique, due to the lack of knowledge of the variety of recipes.

**Conclusions:** It could be noted that there is food waste in this institution due to the lack of instruction regarding the integral use of food, that can be used to reduce waste and enrich the elderly's diet.

**Keywords:** Institution of Long Permanence; Consumption, Waste; Full Use of Food

144/2416

EVALUATION OF FOOD WASTE AND THE PROMOTION OF THE FULL USE OF FOODS IN AN INSTITUTION OF LONG PERMANENCE FOR ELEDERLY (ILPE)

**Background and objectives:** Most of the Long Permanence Institutions for the Elderly (ILPE) in Brazil are philanthropic, and the food comes from individuals and legal entities donations, which could lead to nutritional deficits due to a lack of adequate nutrition planning. However, nutrient deficiencies can be minimized by full utilization of food. This work aims to evaluate and promote the integral utilization of food by a ILPI in Dourados, MS (Brazil).

144/2467

OBESITY: ANALYSIS OF THE USE OF A COMPREHENSIVE SYSTEM OF CARE, THEIR ADJUSTMENTS AND THERAPEUTIC COURSE OF THEIR PATIENTS THROUGH A RETROSPECTIVE STUDY

**Background and objectives:** Background: We understand obesity is closely related to lifestyle; where the context and meanings about commensality and the automatism of habits, represent the backbone that sustains the disease, and in turn these patterns of behavior sustained over time leave a neuroendocrine footprint that demand more therapeutic resources to be modified. Our therapeutic setting is an integral and dynamic interdisciplinary approach through individual consultations and group meetings high frequency, with a multicentric experience of 23 years.

**Objective:** To observe the use of a comprehensive health system by obese patients

**Methods:** Retrospective analysis of 13 years database, Markie, stata sand Microsoft Excel programs were used, in front of therapeutic adaptations implemented in the institution.

<footnote n="1">¹Nursing Faculty, University of Valladolid. Spain.</footnote>

<footnote n="2">²Endocrinology and Clinical Nutrition Research Center (ECN-RC), University of Valladolid. Spain.</footnote>

<footnote>(1)Alonso Balthazar, Emilia; (2)De Souza Araújo, Sarah; (3)Moura de Moraes, Carlos Leonardo; (2)De Souza Araújo, Priscila.</footnote>

<footnote>(1)PhD. Nutritionist. Federal University of Grande Dourados - Faculty of health science - Course Nutrition; (2)Nutritionist;</footnote>

<footnote>(3)graduate student; nutrition; Federal University of Grande Dourados; Faculty of science health.</footnote>

<footnote>(1)Zullich, Karina; (2)Ravenna, Maximo; (2)Olkies, Abel.</footnote>

<footnote>(1)MD General practitioner. Maximo Ravenna therapeutic Centre;</footnote>

<footnote>(2)MD. Maximo Ravenna Therapeutic Centre. Buenos Aires. Argentina.</footnote>
**Results:** Total population 31815 patients (75% female, mean age in years (SD): 44.20 (11.6))

Significant differences between an early period 2003 to 2011 were observed and one late in the 2011-2015 which had already settled many therapeutic adaptations strategic (diversification for monitoring and patient care, multidimensional motivational enhancement, decisive changes in dietary patterns adaptations performance modules, etc. Noting the following differences:

Patients reached the maintenance first time: P1 (P=period of time) = 13%; P2= 53%. Patientes that returned to the system: P1= 1r (r=re-entry): 25%; At least 2r 8.6%; At least 3r : 3%; At list 4r: 1%. P2= 1r: 36%; 2r: 12%; 3r: 2%; 4r: 0.35%. Weight threshold recovered: P1= 90% returned at least 1 time with weight regain but less weight than income; 78% returned at least one more time but with less weight than they had in the first return; 77% less weight than the second return. P2= 92% returned at least 1 time but with less weight than the start; 81% patients returned at least one more time but less than the last weight; 92% returned at least one more time but with less weight previous.

Number of active groups in maintenance: P1= 1; P2= 6. Rate asset maintenance patients: P1= 0.2%; P2= 12%.

**Conclusions:** The adjustments to the professionals to the needs of patients, and maturation of resources has a positive impact on the thresholds and therapeutic goals

**Keywords:** Comprehensive health system obese patients

**Conflict of Interest Disclosure:** It is important to mention for the analysis of implications that the researcher belongs to the institution in which it is investigated, this being the same one that funds the investigation. Private institution that performs activities of private order (individual attention in medical and nutritional clinics and therapeutic groups) and also public order activities (such as collaboration with public canteens, schools, physical activity open to the community in parks)

144/2569

BENEFITS OF DIETARY FIBRE FOR HUMAN HEALTH AND EATING HABITS IN DIFFERENT COUNTRIES

**Background and objectives:** Because DF has unequivocally demonstrated many benefits for the human health, its consumption should be encouraged. In this way, this research aimed at identifying the eating habits regarding fibre rich foods and assessing the knowledge about the effects of DF as enhancers of human

wellbeing. The study was undertaken simultaneously in five countries situated in different parts of the globe, namely South America (Argentina), Africa (Egypt), South Europe (Italy), Central Europe (Romania) and North Europe (Latvia).

**Methods:** A descriptive cross-sectional study was carried out on a non-probabilistic sample of 2290 participants from 5 countries. The data collection was made by a questionnaires translated into the native languages in all participating countries.

**Results:** The consumption of vegetables and salads was higher for Argentina and lower for Egypt while fruits are more consumed in Italy and less in Latvia. Whole grains are more consumed in Latvia and Romania and less in Argentina and Italy. The knowledge about the benefits of DF for cardiovascular diseases and cholesterol is higher in Argentina, for bowel cancer and diabetes in Romania and for obesity and constipation in Latvia. Still, an important number of participants do not have opinions about the different health benefits of DF.

**Conclusions:** In general, the participants showed a moderate consumption of vegetables and fruits but low in whole cereals. The level of knowledge about the effects of DF on human health is still far from desirable levels, and differs considerably from country to country.

**Keywords:** Dietary fibre, eating habits, fibre rich foods, questionnaire survey

144/2587

FOSTERING SCIENTIFIC RESEARCH ON FOOD AND NUTRITION IN SCHOOLS. EXPERIENCE AT THE "TORRENT DE CAN CARABASSA" SCHOOL IN BARCELONA

**Background and objectives:** The Catalan Foundation for Research and Innovation (FCRi) participates in the project "Amgen Exper(i)encia" within a European program, to provide a more complete and up-to-date scientific and technical education to our young people through the participation of retired scientists in the school centres.

**Methods:** The school "Torrent de Can Carabassa" in Barcelona is one of those participating in this program. 39 students with a mean age of 10 years participated in this program with retired pharmaceutical researchers in the field of food and nutrition. A survey was conducted to evaluate healthy habits of these children, regarding physical activity and diet, to focus on the scientific aspects in this field.

The intervention consisted in several talks, debates and workshops with a duration of 8 hours distributed in two months, with participation of students and professors.

**Results:** The discovery of the importance of research and the knowledge about relevant scientists gave as a result that 79% of

<footnote>(1)Leal, Marcela; (2)Guiné, Raquel; (3)Tarcea, Monica; (3)Fazakas, Zita;</footnote>

<footnote>(4)Vittadini, Elena; (5)Klava, Dace; (6)EL-Kenawy, Ayman.</footnote>

<footnote>(1)MSc. Faculty of Health Sciences. Maimonides University. Argentina.; (2)PhD. CI&DETS Research Centre. Polytechnic Institute of Viseu. Portugal.; (3)PhD. University of Medicine and Pharmacy from Tirgu-Mures. Romania.; (4)PhD. Department of Food Science. University of Parma. Italy.; (5)PhD. Latvia University of Agriculture. Jelgava. Latvia.; (6)PhD. Genetic Engineering Institute. University of Sadat City. Egypt.</footnote>

<footnote>(1)Rivero Urgell, Montserrat; (3)Chifré Petit, Ricard.</footnote>

<footnote>(1)Catalan Foundation for Research and Innovation (FCRi). Barcelona. Spain. Royal Academy of Pharmacy of Catalonia (RAFC). Spain. Catalan Academy of Food Sciences. Spain.;</footnote>

<footnote>(3)Catalan Academy of Food Sciences. Spain.</footnote>
children expressed interest in being scientists as a future profes-
sion. Among the high quantity of results obtained from the sur-
vey, it should be noted that 55% of the children ate sweets or buns
daily. 45% did not achieve the recommended intake of fruits and
vegetables. 48% dedicated 2 to 3 hours per day to watch TV, play
at the computer or console, and 21% did not perform any physical
activity outside school.

**Conclusions:** Learning about science and the introduction to the scientific method in the classroom encourages scientific vocations. The collaboration of scientists with teachers has been much appreciated and is a great tool to introduce science in classrooms. It is possible to improve the knowledge of the children in the mistaken habits detected.

**Keywords:** Education. Science. School

**Further collaborators:**

Catalan Foundation for Research and Innovation (FCRi). Bar-
celona. Spain.

144/2625

THE RELATIONSHIP BETWEEN GENDER, BODY IMAGE, BODY SIZE AND REMOTENESS WITH DISCRETIONARY INTAKE AMONG A NATIONALLY REPRESENTATIVE SAMPLE OF INDIGENOUS AUSTRALIANS

(1)Fayet-Moore, Flavia; (2)McConnell, Andrew; (3)Kim, Jean; (4)Petocz, Peter.

(1)MNutrDiet, PhD. Nutritionist. Nutrition Research Australia;

(2)HBSc. Mathematician. Nutrition Research Australia. Australia;

(3)PhD. Scientist. Nestlé Australia. Australia; (4)PhD. Associate Professor. Department of Statistics. Macquarie University. Australia.

**Background and objectives:** Over a quarter of the Burden of Disease among the Australian Indigenous population can be reduced by eliminating poor diet, alcohol and high body mass. Adolescents have the greatest intake of nutrient-poor foods and beverages and are more likely to have body image issues. We aimed to determine the relationship between discretionary food intake among adolescents and demographic, geographic, anthropometric and body image variables.

**Methods:** Day 1 data from 12-18 year olds in the 2012-13 National Aboriginal and Torres Strait Islander Nutrition and Physical Activity Survey were used (n=588). Discretionary foods and beverages (DF) are high in saturated fats, added sugars, and/or salt. Waist circumference to height ratio classified participants' risk of metabolic complications. Remoteness, body image, dieting and DF contribution to total energy were analysed.

**Results:** Prevalence of being at increased risk of metabol-
ic complications was significantly higher among boys (42.6%)
than girls (28.2%) (P<0.05), and all boys (100%) who perceived
themselves overweight were at risk of metabolic complications
compared to 58.8% of girls. 9.6% of girls were dieting compared
to 4.1% of boys, and dieting was higher among those at risk of

metabolic complications (19.4% of girls, 10.3% of boys). 55.0% of
girls and 17.6% of boys who perceived themselves overweight were
on a diet. DF contributed 39.9% of total energy, almost half came
from lunch (25.1%) and dinner (24.2%), was similar between boys
(40.9%) and girls (38.9%), and was significantly lower among
those from remote (33.5%) compared to non-remote (41.5%) are-
as (P<0.05). The energy contribution from DF was higher among
boys who perceived themselves overweight (56.3%) than girls
(26.9%), and higher among girls who perceived their weight as ac-
ceptable (43.6%) than boys (39.7%).

**Conclusions:** Boys were more likely to be at risk of metabolic complications, and those at risk had a greater discretionary contribution than those not at risk. By contrast, girls at risk of metabolic complications had a lower contribution from DF than those not at risk. These results support a need to reduce DF intake among Indigenous adolescents, and to implement gender specific strategies related to dieting and body image, taking remoteness of location into account.

**Keywords:** Indigenous, adolescent, body weight, body image, discretionary foods

**Conflict of Interest Disclosure:** This research was funded by Nestlé Australia.

144/2646

ASSESSING THE EFFECT OF THE PLATE COLOUR ON ENERGY INTAKE DURING AN OPEN BUFFET MEAL

(1)Akyol, Asli; (2)Samur, Gulhan; (3)Eroglu, Elif; (3)Cetin, Cansu; (2)Ayaz, Aylin.

(1)PhD. Lecturer. Hacettepe University, Department of Nutrition and Dietetics, Ankara. Turkey; (2)PhD. Lecturer. Department of Nutrition and Dietetics. Hacettepe University. Turkey.; (3)Research Assitant. Department of Nutrition and Dietetics. Hacettepe University. Turkey.

**Background and objectives:** Recent research suggests that the plate colour may affect the amount of the food consumed. More specifically, colour red was shown to reduce the intake of food due to its act as a consumption-stopping sign. In light of this, the main objective of this study was to determine whether the food consumption during an open buffet meal was different when using same sized white, red or black plates.

**Methods:** This was a cross-over study conducted on 54 female participants aged 18-27 years with normal BMI levels. Participants were recruited from Hacettepe University and the surrounding community. On experimental days, participants ate a standard breakfast and were then randomly assigned to eat lunch (pasta with tomato sauce and soft drinks) using white, red or black plates. Energy and macronutrient intakes during lunch were recorded.

**Results:** Results indicated that plate colour exerted a signifi-
cant effect on food intake during the test days (p=0.021). The av-
erage total energy intake on red (1102.16±47.12 kcal, p=0.05) and
black plates (1113.19±47.12 kcal, p=0.034) were significantly in-
creased when compared to white plate (945.72±47.12 kcal). There was no difference between red and black plates (p=0.985).

**Conclusions:** In this study, colours red and black did not induce a suppressing effect on food intake. These results supported the notion that the food colour is also a crucial determinant of energy intake. The contrast between the plate colour and the food colour should be considered as an important factor when making recommendations about this topic.

**Keywords:** Plate colour, energy intake, satiety response

during meals, and enjoying meals with family are characteristics associated with a positive QOL.

**Keywords:** Middle school students, quality of Life (QOL), family meal

**Conflict of Interest Disclosure:** Conflict of Interest of the Principal Presenter: No potential COI to disclose

**Further collaborators:**

This work was supported by JSPS KAKENHI Grant Number JP 16K16297.

144/2671

## CHARACTERISTICS OF MEALS IN HOUSEHOLDS WITH MIDDLE SCHOOL STUDENTS WHO RARELY EAT BREAKFAST WITH THEIR FAMILIES THOUGH HAVE A POSITIVE QUALITY OF LIFE

(1) Sakamoto, Tatsuaki; (2) Hosoda, Kouhei.

(1) PhD. Department of food and health Sciences. Faculty of environmental and symbiotic sciences. Prefectural University of Kumamoto. Japan.; (2) MLS. Department of health and nutrition. Faculty of human life studies. University of Niigata Prefecture. Japan.

**Background and objectives:** Family meals frequency link healthy dietary behaviors and quality of life (QOL). This study was made to clarify the characteristics of meals in households with middle school students who rarely eat breakfast with their families and have good QOL.

**Methods:** A survey was conducted in September 2015 of 797 second year middle school students in seven schools in Fukui Prefecture, Japan and the results of valid responses from 762 students were analyzed. The questions covered general attributes, the way students ate meals with their families, their enjoyment of meals, and their QOL. Regarding the way students ate meals with their families, the survey asked about how often breakfast and dinner were eaten together with their families, the duration of dinner on weekdays and weekends, whether families listened to them during meals, and how often families scolded them during meals. For QOL measurement, a QOL assessment designed for middle school students (Kiddo-KINDLR) was used. The students who responded that they “almost never” ate breakfast with their families were divided into a low QOL group and a high QOL group based on median total QOL scores of all participants. The study compared the way students in each of these groups ate with their families.

**Results:** Of the 300 students who almost never eat breakfast with their families, 63 male and 56 female students were in the high QOL score group. The high QOL score group ate dinner with their families more often than the low QOL score group, were more often listened to by their family members during meals, were less often scolded during meals, and more students in this group reported enjoying eating with their families.

**Conclusions:** These results suggest that among middle school students who rarely eat breakfast with their families, eating dinner together frequently, being listened to by family members often

144/2684

## RESCUING FOOD CULTURE WITH EDUCATIONAL STRATEGIES IN THE SCHOOL ENVIRONMENT: INTERSECTORAL ACTION BETWEEN HEALTH AND EDUCATION

(1) Andrade, Samantha Caesar de; (2) Santos, Camila Vieira dos;

(3) Machado, Maria Elizabeth; (1) Vieira, Viviane Laudelino.

(1) PhD in Science. Food and Nutrition Reference Center (CRNutri). Teaching Health Center Geraldo de Paula Souza. School of Public Health - University of São Paulo. Brazil.; (2) Undergraduate in Nutrition. Department of Nutrition. School of Public Health. University of São Paulo. Brazil.; (3) PhD in Science. Nutritionist and teacher. Department of Nutrition. School of Public Health - University of São Paulo. Brazil.

**Background and objectives:** The relationship between school and basic health unit strengthens food and nutrition education activities (FNE) from the perspective of an integral development, interfering in the culture and in the social production of health. This article takes educational strategies in order to rescue food culture in the school environment, linking health and education.

**Methods:** CRNutri belongs to the Teaching Health Center Geraldo de Paula Souza of the University of São Paulo, Brazil - and it aims to promote health together with the prevention and treatment of diseases related to food and nutrition. During 2016, nutritionists and nutrition undergraduates, acted collectively in order to rescue food culture at a Children's Educational Center (CEC), in the Teaching Health Center area. The activity has reached all CEC team, joining board, pedagogical coordination, educators, and administrative officials, in addition to 56 children participants.

**Results:** Five strategies were used to build a multidisciplinary and shared knowledge and to rescue food culture in the school environment: 1) Motivational Meeting with the team of the CEC, using dynamics to create links, identify knowledge, myths and activities already carried out on food and nutrition and raise expectations; 2) Motivational meeting with the child's guardians, dynamics development to enhance the shared construction and the importance of different social roles; 3) Application of a questionnaire to the child's guardians to identify family eating habits/culture, directing the two proposed practical workshops with staff and students in order to expand food recipe options through regional food and with children, encourage the consumption of
by 2.6% in girls (25.4% obese female) and a 2.3% in children (4.4%
children obese).

**Conclusions:** The implementation of educational interven-
tion is an effective tool to improve eating habits at school as an
initiative of individual empowerment. The previous identification
of healthy lifestyle habits in schoolchildren; It allowed to design,
implement, and evaluate educational intervention, which through
an integrated to the Act of teaching and learning process helped
promote healthy habits in schoolchildren and consustancialmente
raised the level of knowledge

**Keywords:** Childhood obesity, school children, dietary habits

**Conflict of Interest Disclosure:** It was felt that the design of the study was the most suitable due to the characteristics of the programme for the promotion of healthy living, and also the duration of the same. The reason for not choosing a group on which the educational intervention is carried out and compare it to another without training talks, was due to the low number of students within the centres at each educational level

**Further collaborators:**

Teachers from schools in Ourense and Vigo

144/2742

EFFECTIVENESS OF AN EDUCATIONAL IN-
TERVENTION ON LIFE HABITS ASSOCIATED
WITH SCHEDULE TV CONSUMPTION DAILY IN
SCHOOL CHILDREN FROM 6 TO 12 YEARS

(1)Menor Rodríguez, Maria Jose; (2)Aguilar, Maria Jose; (3)Perez, Maria Teresa.

(1)Director of nursing; (2)Catedrática Enfermeria. Universidad de Granada; (3)Bioestadistica. Colegio de Enfermería de Ourense.

**Background and objectives:** During childhood and adoles-
cence conform the main features of learning, thus acting in these
ages is key to obtain a greater impact on the development to future
of certain diseases.

Objective: To determine if an educational intervention carried
out by a health professional improves the habits of life associated
with the consumption of hours of tv daily in school children 6-12
years and check their relationship with the presence or not of tele-
vision in the child's room.

**Methods:** A written pre-test consisting of questions about healthy lifestyle habits associated with the use of tv, internet and the existence or not of tv and computer in the fourth and socio-demographic characteristics was given to 322 students from 6-12 years of Orense. Subsequently, was an educational intervention supported by videos and informative talks about the risks associated with an elevated daily tv consumption. Two months later was reassessed through a pos test habits.

**Results:** After the educational intervention, the intervention group students have improved their consumption habits of daily tv by 44% with respect to the pre test.

**Conclusions:** Education about healthy lifestyle habits activi-
ties implemented by health care professionals provide an increase

in knowledge about our school, it would be so interesting to com-
plement the training with such activities

**Keywords:** Habits of healthy lives, school, Television

**Conflict of Interest Disclosure:** The results obtained in this study reflect that age may have influenced positively in the acquisition of healthy lifestyle habits in schoolchildren after the educational intervention. It is important to also highlight an adequate environment organization, including spaces, material resources and time distribution, was fundamental to the achievement of the proposed objectives

**Further collaborators:**

Teachers College Xunqueira Ourense

144/2813

DEVELOPMENT OF A SMARTPHONE APPLICA-
TION FOR HEALTHY NUTRITION HABITS AND
PHYSICAL ACTIVITY FOR PREVENTION OF
WEIGHT GAIN IN CHILDREN

(1)Reininger, Mara Edith; (2)Minuzzi, Pedro Gustavo; (3)Katz, Mónica;

(4)Pentimalli, Hernan.

(1)MS Software Engineering. Universidad Nacional de la Matanza. Bs. As. Argentina. Seis Grados Salud. Argentina; (2)PhD Universidad Nacional de Córdoba. Córdoba. Argentina. Seis Grados Salud. Argentina; (3)PhD Nutricionista Universidad de Bs. As. Buenos Aires Argentina; (4)Técnico Informático UBA Buenos Aires Argentina. Seis Grados Salud. Argentina.

**Background and objectives:** Background

Current generations of children and teenagers are considered
mobile first, they use smartphones as primary source of enter-
tainment, social media and information consumption. There is an
ongoing child obesity epidemic that's being addressed using tra-
ditional treatment or prevention strategies based on classic media
channels leaving these new digital tools underexplored and used.

Objectives

The purpose of this study was to evaluate and generate a func-
tional product description to develop a smartphone application
scientific based for children that maximizes the adoption, usage
and retention rate while strictly following the medical objective of
spreading healthy nutrition habits in children aged 10-12.

**Methods:** Gamification strategies were explored as the chosen age range has a natural ludic tendency. A cartoon character-based strategy was selected as it had the best feedback during early reviews with children. The set of medical requirements was worked through a multidimensional matrix including positive and negative emotions, physical self awareness, nutritional information, exercise levels producing a dynamic schedule of games, contents, contacts using push notifications and other activities. A list of the top 100 both commercial and government-backed smartphone applications were evaluated. The list was built using the highest ranked applications from the Apple's App Store and Google Play store.

**Results:** Application's full specifications were developed, in-
cluding wireframes, high definition screen and screen transitions,
functional descriptions, process rationales and medical argumen-
tations for critical decisions.

**Conclusions:** The system developed during this study might provide a unique opportunity to prevent weight gain in children in a highly cost-effective basis. A positive feedback loop of test-feed-back-development, as it is usual in this type of processes, will be necessary.

**Keywords:** Child obesity, smartphone, physical activity, health education.

**Further collaborators:**

Fernando Deleau Bachelor of Business Administration Bue-
nos Aires. Argentina. CEO Seis Grados Salud. Argentina

equate knowledge, were breastfeeding their babies, started breast-
feeding at the hospital and breastfed for the first hour of the baby's
life. The evaluation made it possible to verify the importance of
carrying out educational actions with this population on the reali-
zation of adequate breastfeeding.

**Keywords:** Breastfeeding. Knowledge. Women. Family Health Unit.

**Further collaborators:**

Pro-Rectory of Extension and Culture (PROEX) of the Federal University of Grande Dourados.

144/2831

KNOWLEDGE AND REALIZATION OF BREAST-
FEEDING BY WOMEN OF A FAMILY HEALTH UNIT
IN THE CITY OF DOURADOS-MS

(¹)Lima, Cláudia; (¹)Basile, Lívia; (²)Rodrigues, Sandra.

(¹)PhD. Nutritionist. Nutrition Course. Federal University of Grande Dourados. Brazil.; (²)Nurse. Family Health Unit Novo Horizonte. Department of Health. Brazil.

**Background and objectives:** Currently, according to the World Health Organization, many scientific studies demonstrate the relevance of breastfeeding for maternal and child health and for spacing gestations as a factor of decreased fertility. The objective of this study was to evaluate the knowledge and the realization of breastfeeding by women of a Family Health Unit in the city of Dourados-MS.

**Methods:** The study was carried out with 23 women, who participated in an extension project entitled "Promotion of Breastfeeding - PROAMA II", registered in SIGProj under number 218095.1107.199203.18112015. Before the initiation of educational actions, a diagnostic evaluation was applied through a questionnaire to characterize the population and verify the knowledge and realization of breastfeeding by these women.

**Results:** Of the women evaluated, the mean age was 25.4 ± 6.4 years, 47.8% had primary education, 39.1% had only one child, 56.5% had previously breastfed and 87.0% had been breastfed. It was observed that 95.7% reported knowing the signs of correct baby's handhold on the breast, however, only 21.7% knew how to respond adequately when questioned about these signs. Of the women evaluated, 47.8% reported that the baby is always breastfeeding and 30.4% give the breast when the baby is hungry. Regarding breastfeeding, 96.7% were breastfeeding at the time of the evaluation, 100.0% started breastfeeding at the hospital, 78.3% breastfed during the first hour of the baby's life, 50% were mixed breastfed, 65.2% had help in the first feeding, and for 86.7% this help was provided by the nurse. It was also observed that 91.3% of the women reported that the first time they breastfed was more pleasant than they had imagined.

**Conclusions:** Most women reported knowing the signs of cor-
rect baby's handhold on the breast, although they did not have ad-

144/2839

ANTHROPOMETRIC PROFILE AND NUTRITION-
AL EDUCATION FOR INDIGENOUS SCHOOL-
CHILDREN IN THE CITY OF DOURADOS-MS

(¹)Lima, Cláudia; (¹)Basile, Lívia; (²)Souza, Gabriela; (²)Marques, Giselle.

(¹)PhD. Nutritionist. Nutrition Course. Federal University of Grande Dourados. Brazil.; (²)Student. Nutrition Course. Federal University of Grande Dourados. Brazil.

**Background and objectives:** The city of Dourados has the second largest indigenous urban reserve in Brazil, with approximately 11 thousand indians. Many are the factors by which the lifestyle of these peoples had great modifications, culminating both in the commitment of their food security and in the consumption of industrialized foods, especially sugar. The objective of the present study was to do anthropometric evaluation of indigenous schoolchildren and promote healthy eating habits for these students.

**Methods:** It is an extension project, registered in SIGProj about number: 180319.878.185668.19052014, done with forty seven students of the fourth year of an indian school in the city of Dourados-MS. In 2016 four nutrition education workshops were started with the presentation and explanation of the theme, followed by games to fix the content. An anthropometric evaluation of weight and height was also performed at the first and last meeting. The topics covered were Personal Hygiene, New Food Guide for the Brazilian Population, Food Builders and Food Labeling. Some methods used were: handshake dynamics, handwashing with gouache paint, food tray, food semaphore, posters, games and shopping list.

**Results:** The processed foods were the ones that generated the most doubts among the students. Milk and dairy products and beans were not recognized as protein sources by most students. It was observed that 78.3% of the students had between 70 and 100% of hits in the shopping list. In the initial anthropometric evaluation, 100% of the students had adequate weight for age and height for age, and according to BMI for age, 64.8% were eutrophic, 31.9% overweight and 4.2% obese. In the final evaluation, 100% had adequate weight for age, 97.5% adequate height for age, and according to BMI for age, 65.8% were eutrophic, 29.2% overweight and 5.0% obese.

**Conclusions:** The results obtained with this work demonstrate the importance of continuing the development of nutritional ed-
ucation activities with indigenous schoolchildren, in order to en-
able them to make good food choices and to stimulate hygiene
habits, preventing chronic and infectious parasitic diseases and
controlling the development of overweight and obesity in this
population.

**Keywords:** Nutritional education. Indigenous. Schoolchil-
dren. Overweight.

**Further collaborators:**

Pro-Rectory of Extension and Culture (PROEX) of the Feder-
al University of Grande Dourados. Students: Ana Paula de Jesus,
Marisa Ramos, Patricia Freitas, Thaina de Oliveira, Lais Garcia,
Rafaela Souza e Ludmilla Cabral.

144/2945

CULTURAL DOMAIN ANALYSIS: FINDINGS FROM
FORMATIVE IMPLEMENTATION RESEARCH IN
NORTHERN SENEGAL

**Background and objectives:** Infant and young child feeding (IYCF) practices are a product of culture and context. Effective IYCF interventions should be grounded in formative implementation research that provides an understanding of caregivers' knowledge systems and values that inform their complementary feeding practices. The objective of this study was to examine cognitive frameworks of Senegalese caregivers with respect to IYCF using cultural domain analysis techniques.

**Methods:** Selected modules of the Focused Ethnographic Study for Infant and Young Child Feeding Manual were administered to a sample of 46 caregivers from rural (N=22) and peri-urban (N=24) communities in Saint-Louis, Senegal. Respondents first rated 38 food items on a scale of 1 (low) to 5 (high) according to five dimensions that were hypothesized to influence IYCF: appeal, child acceptance, convenience, healthiness, and modernity. Then, respondents were asked to group the 38 foods into piles based on their own sorting criteria. Data analysis was conducted using R-Cmap, a concept-mapping technique included in the R analytic package. Multidimensional scaling (MDS) analyses produced graphical outputs of aggregate food clusters, separately for each location. Cluster rating maps were produced by combining the mean food rating values with the clusters generated by MDS, and comparison of statistical significance between clusters was conducted using the Tukey method. Rural and peri-urban samples were analyzed separately.

**Results:** The analysis showed that peri-urban respondents identified five clusters ("foods that are good for the body," "snack foods," "light foods," "heavy foods," and "packaged foods"), and rural respondents identified four clusters ("heavy foods," "snack foods," "light foods," and "foraged foods"). In the rural community, "snack foods" received the highest mean rating across all value dimensions, whereas "foraged foods" received the lowest mean rating on all dimensions except convenience. In contrast, the peri-urban community rated "packaged foods" highest across all dimensions except appeal.

**Conclusions:** This study reveals both similarities and differences between the knowledge frameworks of caregivers in the rural and peri-urban study sites, as well as broader differences in both from biomedical concepts of nutrition. These findings provide instructive information to inform the design of complementary feeding interventions in northern Senegal.

**Keywords:** Cultural domain analysis, Senegal, implementation research, pile sort, rating

144/2946

COGNITIVE MAPPING TECHNIQUES REVEAL
CULTURAL INFORMATION ON INFANT AND
YOUNG CHILD FEEDING DECISIONS TO INFORM
DESIGN OF NUTRITION INTERVENTIONS IN
NORTHERN SENEGAL

**Background and objectives:** Caregivers of infants and young children make decisions about feeding their children based on complex interactions of knowledge, beliefs, and values, as well as situational determinants, such as economic and social constraints and opportunities. Because of the relationships and influences of these factors on adopting new infant and young child feeding behaviors, the design and development of nutrition interventions must be grounded in knowledge about the target population. This study used cognitive mapping techniques to gain insight into caregivers' knowledge and perceptions of local foods for infants and young children and examined their potential significance for feeding decisions in northern Senegal.

**Methods:** Guided by mixed methods protocols from the Focused Ethnographic Study for Infant and Young Child Feeding Manual, in-depth interviews that included qualitative discussions

<footnote>(1)Parker, Megan E Parker; (2)Zobrist, Stephanie; (3)Pelto, Gretel;</footnote>

<footnote>(4)Diallo, Abdoulaye Moussa; (5)Kalra, Nikhila; (6)Ndoye, Tidiane;</footnote>

<footnote>(7)Wone, Issa; (8)Amundson, Kimberly; (9)Milani, Peiman; (10)Kreis, Katharine.</footnote>

<footnote>(1)PATH; (2)BA, MPH. Nutrition Innovation. PATH. USA.; (3)BA, MA, PhD. Division of Nutritional Sciences. Cornell University. USA.;</footnote>

<footnote>(4)BA, MA. Cheikh Anta Diop University. Senegal.; (5)BA, MSc, PhD. Nutrition Innovation. PATH. USA.; (6)BA, MA, PhD. Cheikh Anta Diop University. Senegal.; (7)MD, MSc, MPH. University Assane Seck. Senegal.; (8)BS, MSPH, RDN. Maternal, Newborn, Child Health and Nutrition. PATH. USA.; (9)BSc, MSc, MBA. Sight and Life Foundation. USA.; (10)BS, MPH. Nutrition Innovation. PATH. USA.</footnote>

<footnote>(1)Zobrist, Stephanie; (2)Pelto, Gretel; (3)Parker, Megan; (4)Diallo, Abdoulaye Moussa; (5)Kalra, Nikhila; (6)Wittenbrink, Brittney;</footnote>

<footnote>(7)Ndoye, Tidiane; (8)Wone, Issa; (9)Milani, Peiman; (10)Kreis, Katharine.</footnote>

<footnote>(1)BA, MPH. Nutrition Innovation. PATH. USA.; (2)BA, MA, PhD. Division of Nutritional Sciences, Cornell University. USA.; (3)BSc, MSc, PhD. Maternal, Newborn, Child Health and Nutrition. PATH. USA.; (4)BA, MA. Cheikh Anta Diop University. Senegal.; (5)BA, MSc, PhD. Nutrition Innovation. PATH. USA.; (6)BSc, MSc. Nutrition Innovation. PATH. USA.; (7)BA, MA, PhD. Cheikh Anta Diop University. Senegal.; (8)MD, MSc, MPH. University Assane Seck. Senegal.; (9)BSc, MSc, MBA. Sight and Life Foundation. USA.; (10)BS, MPH. Nutrition Innovation. PATH. USA.</footnote>
and cognitive mapping techniques were conducted with 46 car-
egivers in rural and peri-urban communities of Saint-Louis, Sen-
egal. We explored caregivers' perceptions about five dimensions
that were hypothesized to affect food decision-making (health-
iness, convenience, child acceptance, appeal, and modernity),
examining perceptions of 38 food items in relationship to these
dimensions. Data analysis entailed a combination of qualitative
thematic analysis and descriptive statistics.

**Results:** In both communities, “healthiness” was the most valued dimension for food decision-making by a large margin (total mean rating[SD] of 4.7[0.8]), followed by child acceptance (3.6[1.1]), appeal (2.7[1.2]), modernity (2.6[1.0]), and convenience (2.5[1.5]). We explore how different interpretations of these dimensions, and their relationships to local foods, may influence the design and planning of nutrition interventions. For example, respondents almost unanimously associated healthiness, the most highly valued dimension, with cleanliness. With respect to specific foods, eggs, watermelon, Cerelac, and the generic category of vegetables were all rated highly (mean rating >4.5) as healthy foods by both rural and peri-ruban communities.

**Conclusions:** Caregivers weigh many competing factors when making decisions about foods for infants and young children, particularly in low- and middle-income settings where choices are limited and resources are scarce. This study describes caregiver perceptions in two Senegalese communities using a mixed methods, ethnographic approach that illustrates simple and efficient techniques to illuminate the emic perspectives of caregivers, which can be used in designing nutrition interventions.

**Keywords:** Senegal, food decision-making, focused ethnographic study, implementation research, cognitive mapping

144/2970

FOLIC ACID FORTIFICATION OF CORN MASA PRODUCTS: ASSESSING THE CONSUMPTION, IMPORTANCE OF FORTIFICATION AND NEED OF NUTRITION EDUCATION AMONG HISPANIC POPULATION LIVING IN NEW MEXICO, USA

(1)Diaz Campos (Phillips), Sylvia G.; (2)Lee, Deanne.

(1)PhD Student. Msc. Registered and Licensed Dietitian. Family and Consumer Sciences Department. New Mexico State University. United States of America.; (2)MSc Student. Dietetic Intern. Family and Consumer Sciences Department. New Mexico State University. United States of America.

**Background and objectives:** Folic acid (FA) intake is a key micro nutrient for human cell metabolism. One of the most striking consequences of FA insufficiency is a birth defect called: Spina bifida. Arth et al (2016) reported, spina bifida and anencephaly are preventable with sufficient FA intake and “mandatory folic acid fortification of 200 µg/day” has reduced the incidence of birth defects to a minimum of 0.5 per 1000 births. A remarkable reduction, however Latin American descent population might not get enough folic acid through corn masa products which is a staple

for the population. The US Food and Drug Administration (FDA) in 2016 approved for manufacturers voluntarily to fortified corn masa flour products, such as corn tortillas.

Objectives:

1.- Understand the major sources of folic acid and folate and identify the most commonly consumed corn masa product among Hispanics of Southern New Mexico (NM), USA.

2.- Assess FA fortification need and education among residents and corn masa products (CMP) manufacturers.

**Methods:** The descriptive research surveyed Hispanic residents of Southern NM about their diets with a 24-hour food recall. Calculation of FA intake was assessed, 36 different food items like natural sources of folate, FA fortified wheat products, and non-fortified corn masa products. Food data bank from USDA was used. Data was collected from population, ages 18-85.

**Results:** 42 surveys were gathered from the region. Participants consumed an average of:

5.7 servings of non-fortified corn product per day (70%), compared to 2.4 servings of fortified wheat products (30%).

407 mcg of folate of natural sources, 404 mcg of FA fortified wheat products & 135 mcg of FA non-fortified corn masa products (CMP).

The most common non-fortified CMP was corn tortilla.

**Conclusions:** The Hispanic population continues with corn products as a staple in this part of the USA.

Consumption of non-fortified corn products is greater (70%) than fortified wheat products (30%). This is a concern, especially in child-bearing age women, Hispanics have a higher risk of FA deficiency birth defects. It's important to educate young hispanic population on how to read food labels and identify FA fortified CMP. Education of CMP manufacturers will be key as well.

**Keywords:** Corn masa products, Hispanics, Folic Acid fortification, Birth defects prevention

**Further collaborators:**

New Mexico State University, Plant and Environmental Sciences Department.

144/3003

A THREE PRONGED APPROACH TO E-LEARNING:
THE NEED FOR NUTRITION EDUCATION/INNO-
VATION PROGRAMME

(1)Bradfield, James; (2)Ray, Sumantra; (3)Kohlmeier, Martin; (4)Bhat, Shivani.

(1)Nutrition Science Intern. NNEdPro Global Centre for Nutrition and Health. Cambridge. United Kingdom; (2)Chair. NNEdPro. Cambridge. United Kingdom.; (3)Professor. UNC Chapel Hill. United States of America; (4)NNEdPro Global Centre for Nutrition and Health. Cambridge. United Kingdom.

**Background and objectives:** Technology is an essential part of almost all aspects of daily life nowadays however the way we teach students has largely remained the same for generations. Blackboards became PowerPoint presentations and chalk became
laser pointers yet students are still trained that learning reams of material from textbooks verbatim is still an effective way to learn coursework and prepare them for the wordforce. The Need for Nutrition Education/Innovation Programme (NNEdPro) is attempting to modernise the way nutrition is taught to students using a three-point plan.

NNEdPro is aiming to use the technology many of us have grown up using daily to teach nutrition education.

**Methods:** A 360 degree assessment of teaching and learning via participant interaction and review, focus group work on various aspects of nutrition education and KAP surveys throughout NNEdPro activities.

**Results:** NNEdPro has established three main platforms to achieve its goal:

(1) Using the Nutrition in Medicine platform at University of North Carolina at Chapel Hill to create the NNEdPro-NIM Global platform. This material will be specially designed for medical professionals only.

(2) Utilising the Virtual Learning Environment (VLE) team at the University of Cambridge to tailor specific video podcasts for medical students through the Nutrition Education Review Group (NERG).

(3) Designing its own platform to present lectures from the annual Summer School in Applied Human Nutrition in an interactive manner. This is the outward facing platform as all members of the public are eligible to apply to attend the Summer School where they would be granted access to this material.

Each of these platforms will present interactive modules rather than simply relying on a video of a lecture.

**Conclusions:** Though NNEdPro has achieved some success so far in the area of e-Learning, it is clear that there is much to do in this area. Through the continued support of its partner organisations, NNEdPro can continue to pursue the aim of improving nutrition education among medical professionals, student and the general population.

**Keywords:** Education, technology, innovation

**Background and objectives:** Adventist Development and Relief Agency (ADRA) implemented a USAID funded project (Jenga Jamaa II) from 2012 to 2016 aimed at improving food security in Eastern Democratic Republic of Congo (DRC). Johns Hopkins Bloomberg School of Public Health was the operations research partner for the evaluation program activities. As part of this project, women empowerment groups (WEGs) were established to

enhance the business skills and bargaining power of women, their knowledge on proper family nutrition, leadership and life skills. This study focuses on evaluating the effectiveness of the WEGs in improving household food security and nutritional status.

**Methods:** The household food security and nutrition indicators measured included the household dietary diversity score (HDDS) and the prevalence of wasting, underweight and stunting. In each of the 13 WEG villages, one WEG group and one control group of households not participating in WEGs was selected, encompassing a total of 325 households. Baseline and endline data, collected through a structured questionnaire were used for data collection.

**Results:** At the end of the project, 62% of the WEG participants achieved the target HDDS. The mean HDDS significantly increased from 3.40 in the baseline to 5.51 at the endline. The WEGs showed a statistically significant difference in household food insecurity improvement as compared to the control group. Although the mean height for age and weight for age scores among the WEG beneficiaries significantly improved from baseline to endline, there were no significant differences between the prevalence of wasting and stunting in the WEG and control groups at the endline.

**Conclusions:** The study shows that including nutrition activities in women empowerment interventions is a feasible way to improve household food security. However, a detailed analysis of the determinants of malnutrition is necessary to identify other factors contributing to change in nutrition outcomes.

**Keywords:** Women Empowerment Group (WEG), Democratic Republic of Congo (DRC), stunting, dietary diversity, food security.

**Conflict of Interest Disclosure:** The author declares that there is no conflict of interest regarding the publication of this article.

**Background and objectives:** There are numerous terms to define sugars, with an indiscriminate use by the industry in products that claim to be “healthy.” On the other hand, the epidemic of chronic non-transmissible diseases are increasing despite of the availability of these products. Several studies suggest that the consumption of high fructose corn syrup (HFCS) and other added sugars could be compromised in their development.

To record the presence, percentage content, types of sugar and / or their counterparts in products alleged to be “healthy” by the industry.

**Methods:** A descriptive, cross-sectional study was conducted. Data were obtained from the acquisition of products that in their containers contain concepts of “claims” such as:
* Light

* Recommended by scientific societies

* Fiber Source

* Only" x “Kcal

* Food for a better life

* Start or eat well

* Healthy

Available in the most known supermarket chains of the city of Lomas de Zamora during the period of November 2016-February 2017

**Results:** We analyzed 19 types of cookies and cereals, 7 low calorie drinks and 8 snacks, alleged as “healthy” by the industry.

The 94.7% (n18) of the cookies, 85.7% (n6) of the drinks and 100% of the snacks registered the presence of sugar. The 44.4% (n8) of the cookies and 37.5% (n3) of the snacks contain more than 11 grams of sugar in 100 grams of product. The 42.9% of beverages contains less than 10%. The 66.7% of the cookies, 50% (n3) of the drinks and 25% of the snacks (n2) contain 2 to 5 different types of added sugars. (Sugar, HFCS, malt extract, maltodextrin, molasses and glucose syrup).

**Conclusions:** The 93.4% of the analyzed products alleged to be healthy by the industry contain sugar and / or their counterparts. The 47.2% contain more than 2 types of added sugars. The 40.95% of the cookies and snacks have more than 11 grams of sugar in 100g of product.

**Keywords:** Sugar, healthy food, industry

Track 8: Agriculture, Food Science and
Safety

144/278

AGRICULTURAL DEVELOPMENT INTERVEN-
TIONS AND DIETARY DIVERSITY OF RURAL
SMALLHOLDER FARMING HOUSEHOLDS IN
EAST AND SOUTHERN AFRICA (ESA)

(1)Amaka, Marian Odenigbo; (2)Idemudia, Patience.

(1)PhD. Special Adviser on Nutrition. East and Southern Africa Division. International Fund for Agricultural Development.
Italy.; (2)PhD. Professor. Department of Sociology. University of Saskatchewan. Canada.

**Background and objectives:** International Fund for Agricul-
tural Development (IFAD) is proactively promoting nutrition-sen-
sitive agriculture agenda in its operations. To provide guidance for
an effective nutrition mainstreaming in IFAD's work, this study
was conducted to map various nutrition sensitive interventions in
IFAD portfolio; identify pathways for nutrition outcomes; identify
challenges; and areas of opportunities for scaling.

**Methods:** This is a cross-sectional survey conducted in July and August 2016 which involved three countries and five study projects in the ESA region. The investment theme for the various study projects were agribusiness (Zambia); dairy (Kenya); productivity promotion (Zambia); rural marketing (Mozambique); and natural resource management (Kenya). Quantitative data was collected from 402 beneficiary households while qualitative data came from 161 focus group discussants and 34 community key informants (IFAD Country teams, project management staff and implementers). Dietary diversity was evaluated at both household (Household dietary diversity score: HDDS) and individual levels (Minimum Dietary Diversity for women: MDD-W & Minimum Dietary Diversity for children: MDD-C). Multiple Classification Analysis (MCA) model was adopted to relate key project interventions as variables to the dietary diversity.

**Results:** The quantitative and qualitative data indicated that integrated nutrition activities i.e. nutrition education; food demonstration; recipe development; kitchen garden as well as key project investment i.e. diversification of food production; women empowerment; commercialization and access to market; production-focused training had positive effects on dietary profile. The proportion for good dietary diversity was higher for HDDS (63.7-97.3%) as compared to MDD-W (22.5-63.5%) and MDD-C (1.2-47.6%). The MCA analysis showed that HDDS varied significantly according to diversification of services, market linkages, women empowerment, capacity building and literacy status. For the women group, household size, women empowerment and diversification were found as strong determinant factors. Whereas capacity building indicated the most influence on dietary diversity for children.
**Conclusions:** This study conceptualized a simplified framework of integrated impact pathways with distinctive focus on nutrition knowledge, climate and women empowerment as influencers in maximizing impact of agricultural development interventions for improved dietary intake.

**Keywords:** Nutrition-sensitive Agriculture, Diet Diversity, IFAD

**Conflict of Interest Disclosure:** This report is a product of staff of the International Fund for Agricultural Development (IFAD) and the findings and conclusions expressed herein do not necessarily reflect the views of its Member States or their representatives to its Executive Board. IFAD or any of its Member States do not guarantee the accuracy of the data included in this work. The designations employed and the presentation of material in this publication do not imply the expression of any opinion whatsoever on the part of IFAD concerning the legal status of any country, territory, city or area or of its authorities, or concerning the limitation of its frontiers or boundaries.

pH of 4.3 and 21°Brix. Total energy was 138 Kcal/100g, 54% less than traditional prickly pear jam. Chromatic parameters L*, a*, b* and DE (fresh fruit as reference) were 30.98, 7.00, 20.20 and 7.58, respectively. In the sensorial test the flavor showed the lowest score (3.35) perhaps because people are not accustomed to this fruit. Color and brightness had the best scores (3.88 and 3.85, respectively) and to the global acceptance was 3.77. The jam met the microbiological standards of current legislation.

**Conclusions:** This sugar-free prickly pear jam could be a novel option for diabetics and people who want to avoid sugar-sweetened products.

**Keywords:** Key words: jam, tuna fruit, diabetes, sugar-free

144/281

DEVELOPMENT, CHARACTERIZATION AND SENSORY ANALYSIS OF SUGAR-FREE PRICKLY PEAR JAM (OPUNTIA FICUS INDICA)

(1)Gozzi, Marta Sofía; (2)Barroso, Bárbara Malén; (2)Parés, Carolina;

(3)Faggi, Gabriela Marta.

(1)MSc. Food Technologist. Instituto de Tecnología. Universidad Argentina de la Empresa. Argentina; (2)BSc. Food Technologist. Departamento de Biotecnología y Tecnología Alimentaria. Facultad de Ingeniería y Ciencias Agrarias. Universidad Argentina de la Empresa. Argentina.; (3)BSc. Agronomist Engineer. Instituto Nacional de Tecnología Agropecuaria. Estación Experimental Agropecuaria Las Breñas. Argentina.

**Background and objectives:** Diabetes is one of the most common metabolic disorders in the world and its prevalence has been increasing in the last decades. Besides, more and more people are concerned about their health choosing sugar-free products. The aim of this work was to develop a jam with a non-traditional fruit like prickly pear, containing no added simple sugars, and to evaluate their physicochemical, microbiological and sensory characteristics.

**Methods:** The sugar-free jam was made with the whole fruit pulp of orange variety. Stevia was added as a non-nutritive sweetener, and agar-agar and locust bean gum as gelling agents. Sorbitol, maltodextrin, potassium sorbate, ascorbic and citric acid were also added. It was determined the proximate composition, pH, °Brix, and color. Carbohydrate content was estimated by difference. Microbiological analyses were performed. Sensory analysis was taken with 26 non-trained people who evaluated color, brightness, flavor, texture, taste and global acceptance, using a 5-point hedonic scale.

**Results:** The final sugar-free jam contained (g% WB): moisture 62.8, carbohydrate 33.3, protein 1.10, fat 0.9, ashes 1.0, fiber 0.9,

144/292

INVESTIGATION OF THE REACTIONS OF THERMAL PROCESS CONTAMINANTS IN BISCUITS DURING DIGESTION

(1)Hamzalioğlu, Aytül; (2)Gökmen, Vural.

(1)PhD candidate. Food Scientist. Department of Food Engineering. Hacettepe University. TURKEY; (2)PhD. Prof. Food Scientist. Department of Food Engineering. Hacettepe University. Turkey.

**Background and objectives:** Processing of foods cause to the formation of toxic compounds in foods which possess detrimental effects on human health. Acrylamide, 5-hydroxymethylfurfural, α-dicarbonyl compounds are such process contaminants formed as a result of high temperature processing of carbohydrate rich and low-protein crops such as potato, wheat. Besides these contaminants, foods also bear reactive components thus their interactions during digestion is of importance. This study aimed to investigate the reactions of thermal process contaminants including acrylamide, 5-hydroxymethylfurfural (HMF), methylglyoxal (MGO) and 3-deoxyglucosone (3-DG) in biscuits during digestion.

**Methods:** An in vitro enzymatic digestion system simulating gastric, duodenal and colon phases was used and the change in the amount of these compounds was monitored during digestion of different biscuits.

**Results:** Acrylamide levels as well as MGO and 3-DG levels of biscuits gradually decreased through gastric, duodenal and colon phases of digestion. At the end of digestion, acrylamide reduction ranged between 49.2% and 73.4%. Similarly, 3-DG and MGO reduction was found to be varying between 23% and 58.91%, 50.08 % and 77%, respectively. On the other hand, dramatic increase was observed in HMF contents of biscuits at the end of gastric phase. However, with the progress of digestion, HMF concentration significantly decreased, as well. Since these contaminants contain reactive carbonyl groups, reactions with amino and sulfhydryl groups of amino acids might take place. To understand this, model systems composed of contaminants and amino acids including lysine and cysteine were subjected to digestion. The results revealed that disappearance was due to interactions of reactive carbonyl groups with the accumulating amino and sulfhydryl groups
during digestive process. By high-resolution mass spectrometry analysis in model systems and biscuits, the formation of adducts of contaminants with amino or sulfhydryl groups of amino acids were confirmed.

**Conclusions:** In conclusion, simulated digestion conditions favored the addition of amino acids to process contaminants. Owing to its two nucleophilic groups (-SH, -NH2), cysteine becomes highly reactive among the amino acids. Gastrointestinal conditions and the ingested food composition affect the levels of bioavailable process contaminants, and should be taken into consideration.

**Keywords:** acrylamide, HMF, α-dicarbonyl compounds, in vitro digestion, biscuits

144/333

POTENTIAL OF ALTERNATIVE FOOD NETWORKS IN MEDELLÍN

**Background and objectives:** New kind of networks to produce, distribute, and consume foods is being developed in several countries around the world. The alternativeness consists of growing organically produced foods without using a chemical toxic product. The transportation is done with very few intermediaries and short trips, producers have stable and fair price systems and relations between producers and consumers are close and direct. They are called Alternative Food Networks (AFN), which seek to become an alternative to the conventional food system, driven by multinational food producers and big chain retailers, located especially in urban areas. This study aimed to characterize the AFN of Medellín. AFN specifically practices, acts, motivates the camaderie relations among producers, distributors, and consumers, promoting an ethical and professional behavior.

**Methods:** A qualitative study collective cases; semi-structured interviews were conducted with producers and distributors taking part of 12 AFN in Medellín. Assessments of the participants were also carried out at food sales sites to observe interrelations and practices.

**Results:** This study found that in Medellín are incipient AFN experiences. They encompass producers, distributors, and consumers. They sell different products mainly fruits and vegetables. In fewer amounts, they sell: rice, quinoa. Finally, they handmade processed meals like chocolate, cocoa, butter, and cheese. These networks promote local development and use short trade channels because foods are grown in rural Medellín areas or nearby regions. Its consumers are people of high social socio-economic strata and

with high educational level. Consumers of high strata buy this type of food especially to preserve their health, take care of the environment, and support the small producers.

**Conclusions:** There are AFNs existing in Medellín which are of recent creation; they share the alternativeness of other networks around the world and in the future may become an alternative food system that transforms the current hegemonic model.

**Keywords:** Alternative Food Networks, Food supply, Sustainable agriculture, Organic foods, Food production

144/346

EFFECTS OF SEASONALITY ON HOUSEHOLD FOOD SECURITY AND FOOD CONSUMPTION PATTERNS OF PASTORALIST'S CHILDREN IN KAJIADO COUNTY, KENYA

**Background and objectives:** The livelihood of communities in arid and semi-arid lands of Kenya is mainly pastoralism. Climatic seasonality is one of the determinants of food security. Food consumption patterns have been shown to fluctuate depending on the food security situation in a household. Households in ASALs are more likely to be affected by the fluctuations of the seasons which in turn affect food availability. This study aimed to investigate the effect of seasonality on food security and food consumption patterns among children (6 - 59 months) from pastoralists' households.

**Methods:** The study adopted a longitudinal, observational study design, following on households for one year. The study was carried out on 150 randomly selected households in Kajiaado County. Data were collected on a monthly basis for one year. Data on the climatic profile was obtained from the meteorological department. Analysis of variance was used to assess for any differences in food security and food consumption patterns across the seasons. ANOVA was used to analyze for significant differences across different seasons.

**Results:** The dietary diversity score was significantly higher (p < 0.05) during the long rains season (5.13 ± 0.06) as compared to short rains (4.13 ± 0.04) and the dry season (3.45 ± 0.03). During the dry period, the households lacked the capacity to access adequate food due to low production, high food prices and low income from livestock and livestock products. Food scarcity was noted in the markets especially during the dry period due to low supplies. During dry seasons, there was a low frequency of food consumption, less number of meals consumed and reduced nutrient intake among children.

**Conclusions:** Seasonality had a significant effect on food security with the situation becoming worse during the dry season. The change in dietary diversity, the number of meals consumed per day, the frequency of food consumption across seasons was an in-

<footnote>(1)Cadavid Castro, Martha Alicia; (2)Alvarez Castaño, Luz Stella;</footnote>

<footnote>(3)Quintero-Vergara, Shirley Daniela; (3)Martínez-Bedoya, Ximena;</footnote>

<footnote>(3)Martínez-López, Angie Paola.</footnote>

<footnote>(1)Nutritionist. MSc Food and Human Nutrition. Nutrition and Diet School. University of Antioquia. Colombia.; (2)Nutritionist. HD Master in Public Health. Nutrition and Diet School. University of Antioquia. Colombia.; (3)Student. Nutrition and Diet School. University of Antioquia. Colombia.</footnote>

<footnote>(1)Chege, Peter; (2)Muthamia, Olive.</footnote>

<footnote>(1)Department of Food, Nutrition and Dietetics, Kenyatta University, Kenya; (2)Department of Nutrition and Dietetics, Mount Kenya University, Kenya.</footnote>
dicator of how different seasons affected household food security and food consumption patterns. This study recommends promo- tion of food preservation during the plenty seasons, production of drought-resistant crops, initiation of irrigation projects and use of social safety nets during extreme conditions.

**Keywords:** Food Security, Food Consumption Patterns, Seasonality, Arid Areas, Pastoralists

**Background and objectives:** Cattle and hog producers are adding beta agonists to feedstuffs in the United States and Canada that leave residues in meat products consumed by humans. Both the U.S. Food and Drug Administration and a Joint FAO/WHO Expert Committee on Food Additives have decided that low amounts of beta agonist residues do not pose a health problem for humans. Conversely, evaluating nearly identical data, the European Food Safety Authority decided that beta agonists had not been shown to be safe for human ingestion. This research explains how the U.S. and EU have reached different conclusions on the safety of beta agonist residues.

**Methods:** Affect and availability heuristics of animal production practices in the United States and the European Union are analyzed to explain why regulators relying on the same data have adopted divergent provisions for beta agonists. Through the identification of different approaches to animal production, the divergent conclusions reached on the risks of beta agonist residues in food products can be explained.

**Results:** Animal production technologies previously regulated in the U.S. and EU were identified that may account for the discrepancies in the regulatory regimes on beta agonists. Six technologies authorized in the U.S. and limited in the EU offer an explanation for difference conclusions by regulators on risks posed by beta agonist residues in meat products.

**Conclusions:** Regulators in the U.S. had authorized concentrations of animals, tail docking of pigs, castration of male pigs without anesthesia, and the use of hormones, recombinant bovine somatotropin, and non-therapeutic antibiotics. EU regulators had placed limits on these six technologies or banned them. Given these foundations of regulatory controls over food animal production, regulators in the US and EU developed divergent regulatory approaches for beta agonist usage. They weighed risks differently, and cultural beliefs on animal welfare and human health led to different interpretations of data regarding beta agonist residues in food products.

**Keywords:** beta agonists, Codex, U.S. Food and Drug Administration, World Trade Organization

**Background and objectives:** The paper is an attempt at evaluation of the oncoming transformations in the food economy induced by climatic changes. The paper focuses on the regional diversity and arising from the consequences of food security. The basic hypothesis is, climate change, deepening of negative spatial polarization in food production.

**Methods:** The study is based on the analysis of statistical data (FAO, EUROSTAT, ESPON), IPCC reports and cartographic methods.

**Results:** It is assumed that climate warming in the next decades is a phenomenon the most probable to occur. Consequently, it may bring about spatial polarization in the food production - a growth in rich countries (located in the temperate zone) and decrease in poor nations (mainly in the tropical and equatorial areas). In temperate zones the expected changes may result in a growth in potential production of food in view of the increasingly extended vegetative period and widening the geographical ranges of crops. Meanwhile, today is observed in the EU countries the problem of overproduction of food, which seeks to mitigate the CAP. One of the activities is extensification of food production and reduction of agricultural land. In turn, warm areas, including primarily exposed to prolonged droughts increase in average temperatures will bring more negative effects. The most vulnerable continent to climate change seems to be Africa. Limitation of precipitation will strengthen the process of desertification, especially at the edge of the Sahara and south of the continent. This problem is aggravated by the fact that the greater part of its population depends on farming. Present and future interactions between the climate and food security can also be considered in the context of demographic processes. The greatest increases in population will be witnessed in countries that are currently affected by problems in access to food. The result of these processes will be great migrations of population and social conflicts.

**Conclusions:** Climate change probably will not stop. Therefore, it is important to introduce in poor regions a modern methods of food production and to ensure proper distribution of food. We must also be prepared for the major societal challenges.

**Keywords:** food, production polarization, climate change, agriculture
# COGNITIVE BIAS IN FOOD HANDLERS: MORE KNOWLEDGE MEANS FOOD SAFETY?

(1)Gomes de Freitas, Rayane Stephanie; (2)Thimoteo da Cunha, Diogo; (3)Stedefeldt, Elke.

(1)MS student. Nutritionist. Universidade Federal de São Paulo. Brazil.; (2)PhD. Nutritionist. Faculdade de Ciências Aplicadas. Universidade Estadual de Campinas. Brazil.; (3)PhD. Nutritionist. Centro de Desenvolvimento do Ensino Superior em Saúde. Universidade Federal de São Paulo. Brazil.

**Background and objectives:** As food handlers are liable to contaminate food by inappropriate behaviors. In this sense, training is the strategy indicated to instruct them in order to prevent foodborne diseases (FBD) outbreaks. Cognitive biases are hard to prevent and may puzzle correct decisions. The objective of this study is to discuss the relation between knowledge about sanitary norms and cognitive biases.

**Methods:** Ethnography and participant observation were the main strategies used. Participant observation was carried out in six commercial restaurants of two Brazilian cities during 42 days. Information related to foodservice infrastructure, service characteristics, location, details about the agents, their speeches, non-verbal communications, interpersonal relationship, work routine and relation with food safety were written in field diaries. Content analysis of the thematic type was used to elucidate data. It was observed 68 food handlers. Six of them, one of each local, voluntarily answered to questionnaires which provided data about optimistic bias and locus of control.

**Results:** These workers presented an optimistic bias tendency in those questions related to consumer, family and friend's risk of having a FBD when eating a meal which was prepared by them. Despite the disconnection between observed practices and adequate knowledge, those workers showed internal locus of control when they assumed their responsibility for food safety. Optimistic bias and illusion of control seem to manifest only in the presence of knowledge about sanitary norms, since workers felt overconfident. When workers perceive the sanitary risk they may act in a way to prevent it if they know the adequate practices and the negative implications to client's health, as well as if the infrastructural conditions and time are favorable to them. External locus of control corroborates with the belief the occurrence of FBD is not possible and that workers are protected by religious adornments and their beliefs. These situations may increase the risk of FBD.

**Conclusions:** Workers' risk perception becomes permeated by cognitive biases that arise with the help of knowledge about sanitary norms. It is suggested that the training approach does not reach the depth needed to transform the food handlers' thinking and their work environment towards food safety.

**Keywords:** risk perception, cognitive biases, food safety, food handlers, ethnography.

**Further collaborators:**

CAPES - CNPq (National Counsel of Technological and Scientific Development).

# BRAZILIAN FOOD LAW: AN INTEGRATIVE LITERATURE REVIEW

(1)Oneti, Ciro Félix; (2)Ribeiro, Euler Esteves; (3)Ribeiro, Ednéa Aguiar Maia; (4)de Souza Praia, Raquel; (5)Costa, Michelle Silva; (6)Mortágua, Andréa Rebouças; (7)Furtado, Mônica Costeira Mendonça; (8)Nascimento, Lucy Christiane de Souza Hossaine do; (9)Araújo, Arthenize Riamé Praia G. C.; (10)Mesquita, Priscila Lyra.

(1)Enfermeiro. Universidade Aberta da Terceira Idade (Manaus/AM). Brasil.; (2)Médico. Doutorado em Medicina e Ciências da Saúde. Diretor da Universidade Aberta da Terceira Idade (UNATI/UEA). Manaus/AM/Brasil.; (3)Médica. Doutorado em Ciências da Atividade Física e Desporte. Universidade Aberta da Terceira Idade (UNATI/UEA/AM). Brasil.; (4)Doutoranda em Saúde Pública. Universidade de Ciências Empresariales y Sociales (UCES). Enfermeira. Universidade Aberta da Terceira Idade (UNATI/UEA/AM). Brasil.; (5)Assistente Social. Especialista em Política Pública de Atenção a Família. Universidade Aberta da Terceira Idade (UNATI/UEA/AM). Brasil.; (6)Fisioterapeuta. Universidade Aberta da Terceira Idade (UNATI/UEA/AM). Brasil.; (7)Diretora da 1ª Vara da Família do Tribunal de Justiça do Amazonas (TJAM). Bacharel em Direito. Especialista em Processo civil. Assistente Social. Brasil.; (8)Advogada. Assessora Jurídica. Tribunal de Justiça do Estado do Amazonas. Brasil.; (9)Acadêmica de Enfermagem. Universidade Paulista (UNIP/AM). Universidade Aberta da Terceira Idade (UNATI/UEA/AM). Brasil.; (10)Educadora Física. Secretaria de Educação do Estado de São Paulo.

**Background and objectives:** One of the branches of law is in charge of legislating on food-related issues in Brazil. In a country with great territorial extension and still marked by much informality in several sectors of the production chain, it is necessary to act rigorously through a law on the production of foodstuffs to guarantee the protection of consumer health. Objective: To emphasize the elementary importance of the legal mechanisms acting in consonance with inspection agencies in the food production chain in the national territory.

**Methods:** This study is an integrative literature review. The research was carried out on the SciELO platform (Scientific Electronic Library Online), with the descriptors Legislation, Food and Brazil. The platform returned 65 results, the search was filtered searching for texts strictly related to the subject and incomplete texts were duplicated, before the year 2012 or published in a language other than Portuguese, English or Spanish. There remained only nine texts. The research was complemented with textbooks.

**Results:** Brazil has a broad legislative mechanism for the production, packaging, transport, conservation and commercialization of food products. One of the main obstacles to the fulfillment of all determinations is the difficulty of inspection in such a large territory. Added to this is the lack of knowledge of many operational procedures by the producers. It is noted that some themes also acquire bioethical connotations, as in the case of transgenics.

**Conclusions:** This legislative branch will ensure an increase in the quality of national food production, given that the country is
quite heterogeneous. Patent registration benefits greatly from such legislation, and any crimes of this scope end up being inhibited.

**Keywords:** food law; inspection; national food production

144/478

SOCIAL WORK IN FOOD SECURITY FOR THE EL-
DERLY POPULATION

(1)Costa, Michelle Silva; (2)Ribeiro, Euler Esteves; (3)Ribeiro, Ednéa Aguiar Maia; (4)de Souza Praia, Raquel; (5)Neta, Inez Siqueira Santiago; (6)Oneti, Ciro Félix; (7)Mortágua, Andréa Rebouças; (8)Araújo, Arthenize Riamé Praia G. C.; (9)Nascimento, Priscila Hossaine Santos do; (10)Mesquita, Priscila Lyra.

(1)Assistente Social. Especialista em Política Pública de Atenção a Família. Universidade Aberta da Terceira Idade (UNATI/UEA/AM). Brasil.; (2)Médico. Doutorado em Medicina e Ciências da Saúde. Diretor da Universidade Aberta da Terceira Idade (UNATI/UEA). Manaus/AM/Brasil.; (3)Médica. Doutorado em Ciências da Atividade Física e Desporte. Universidade Aberta da Terceira Idade (UNATI/UEA/AM). Brasil.; (4)Doutoranda em Saúde Pública. Universidad de Ciencias Empresariales y Sociales (UCES). Enfermeira. Universidade Aberta da Terceira Idade (UNATI/UEA/AM). Brasil.; (5)Acadêmica de Enfermagem. Universidade Federal do Amazonas. Universidade Aberta da Terceira Idade (UNATI/UEA/AM). Brasil.; (6)Enfermeiro. Universidade Aberta da Terceira Idade (UNATI/UEA/AM). Brasil.; (7)Fisioterapeuta. Universidade Aberta da Terceira Idade (UNATI/UEA/AM). Brasil.; (8)Acadêmica de Enfermagem. Universidade Paulista (UNIP/AM). Universidade Aberta da Terceira Idade (UNATI/UEA/AM). Brasil.; (9)Assistente Social. Especialista em Assistência e Família. Universidade Aberta da Terceira Idade (UNATI/UEA/AM). Brasil.; (10)Educadora Física. Secretaria de Educação do Estado de São Paulo. Brasil.

**Background and objectives:** The determinants of the health of the population, focused on food and nutritional security may be a reflection of the socioeconomic conditions of the population. Data from the 2002/2003 Family Budget Survey (POF) report that there is a prevalence of overweight in low income families in Brazil. Other studies bring an increase in cardiovascular diseases, type 2 diabetes and fixed types of cancer, accentuating morbimortality related to nutritional status. Objective: To identify a role of the social worker inserted in the multiprofessional team of health care, for an insertion of the low income elderly into social food programs of the Brazilian government.

**Methods:** This is a review of the literature in the SciELO database and Virtual Health Library (VHL), 6 studies were selected from the inclusion criteria. Other materials such as books and sites of governmental social programs focused on a theme were also used.

**Results:** The research evidenced that there is a shortage of studies on the performance of the professional social worker of the identifier by the health team of low income elderly people without access to basic foodstuffs and in sufficient quantity, who seek the health service or are assisted in their Home Programs such as fed-

eral government hunger zero, aims to combat hunger and ensure food and nutritional security in low-income populations. However, for the acquisition and consumption of food, development must be done through actions. In this way, as well the Federal Council of Social Service (CFESS), the product in care provided by the social worker, should give through A direct relationship with its users, in relation to the elderly, this with the caregiver / family.

**Conclusions:** The treatment of a multiprofessional and intersectoral work relationship, without social service qualities of the most varied dimensions in which the elderly person finds himself needs a dialogue, appropriate social programs aimed at food security when necessary, in view of the vulnerability For the promotion of health.

**Keywords:** social service; food safety; social programs

144/515

MICROBIOLOGICAL ANALYSIS OF FOOD MANIP-
ULATION SURFACES IN CANTEENS OF A PUBLIC
UNIVERSITY

(1)Mont'alverne Jucá Seabra, Larissa; (2)Pereira Nogueira, Josimara;
(2)Santana Luz, Anna Beatriz; (1)Florentino da Silva Chaves
Damasceno, Karla Suzzane.

(1)PhD. Nutritionist. Department of Nutrition. Federal University of Rio Grande do Norte; (2)Nutritionist. Department of Nutrition. Federal University of Rio Grande do Norte.

**Background and objectives:** Foodborne Diseases (FD) are all clinical occurrences caused by the ingestion of food that may be contaminated with pathogenic microorganisms, chemicals, harmful objects or that contain toxic structures in amounts that affect the health of the consumer, individually or collectively. Food-producing establishments are conducive to microbial contamination. The objective of the study was to evaluate the sanitary quality of the surfaces used for food manipulation in scholar canteens of Brazil

**Methods:** The survey was conducted at 11 canteens located at Federal University of Rio Grande do Norte (UFRN) between February and March of 2016. The study was submitted and approved by the Research Ethics Committee of the University Hospital of UFRN under the number 737.109.

The samples were collected in the canteens using the swab technique and were transported to the Laboratory of Food Microbiology in thermal box with ice bags immediately after their collection to determine the Most Probable Number (MPN) of Coliforms at 45 ° C and Mesophilic Aerobic Bacteria.

**Results:** Coliforms at 45°C were detected in cutting boards used in 63% of the evaluated establishments. The counts were higher than the microbiological sanitary standard in 7 of the 11 canteens investigated. The sites in which they obtained an unsatisfactory classification showed results between 3.0 and 1100 MPN / 50 cm2.

For aerobic mesophilic bacteria, the cutting boards showed a high count in 73% of the canteens classified as unsatisfactory.
For sites that presented unsatisfactory environmental conditions, a minimum and maximum contamination value of 216 and 4600 CFU respectively was observed.

**Conclusions:** The results showed unsatisfactory microbiological quality of the cutting boards utilized in canteens. Considering that food handling surfaces are utensils directly related to the high number of foodborne diseases, this study demonstrates the need to take urgent and effective measures to reduce or eliminate the microbiological contamination found in cutting boards of these food-services.

**Keywords:** Hygienic-sanitary conditions; microbiological analysis; food safety

144/544

## LEVERAGING PRIVATE SECTOR FOR GENDER AND NUTRITION RESPONSIVE AGRICULTURE

Ladd, Nofirstname.

Ladd, BS, MS, RDN. Senior Technical Director, Nutrition. ACDI/VOCA.

**Background and objectives:** Zambia PROFIT+ is a USAID Feed the Future project which operates a market led approach where private sector partnerships drive mutually beneficial development for them and the target communities. A major emphasis in the project is the promotion of job creation through entrepreneurship with the private commissioning Community Agro dealers (CA) to provide services in rural Zambia reaching the most rural areas or "last mile". In addition to input supplies, farm services such as mechanization, and spraying services, the CA provide a variety of training including nutrition, use of improved and nutrient-rich commodities, in addition to creating savings and credit groups. The project trained 334 CA's (28% women), reached over 182,000 smallholder farmers (55% women).

An assessment of the CA model was conducted to determine if the program design and outcomes were reached which included: increased household income, improved production, gender empowerment, and nutrition transformation, as well as improvement in climate smart agriculture practices in the communities.

**Methods:** The assessment included focus group discussions designed to assess the CA model's impact on women's empowerment, nutrition, introduction of nutrient-rich commodities, increased household incomes, and improved production patterns. The interviews involved 286 CAs in 33 groups across the program area.

**Results:** The CA are recognized as role models and change agents in their community. They report an 40% increase in household assets (proxy for income). Farmers working with CA's report a 24% increase in yield as well as increase in production of nutrient rich crops. The Ministry of Health, Ministry of Agriculture, NGOs and private sector leverage the CA's to deliver nutrition and health messages due to their reach into the rural communities or "last mile". In addition, 35% of the women report they are respected as leaders within their communities.

**Conclusions:** Leveraging the private sector to support a market based approach to improve nutrition, increase incomes and improve gender empowerment is sustainable and scalable model thus reducing the need for continued donor funding. By linking CA's with the private sector, this sustainable model reaches rural communities, improves their livelihoods and nutritional status, and increase women's empowerment.

**Keywords:** nutrition-sensitive agriculture, gender, private sector

144/545

## MILKING FOR ALL IT'S WORTH: WOMEN, NUTRITION, AND LIVESTOCK

Ladd, Nofirstname.

Ladd, BS, MS, RDN. Senior Technical Director, Nutrition. ACDI/VOCA.

**Background and objectives:** Livestock raising in developing world offers the potential to significantly increase incomes and improve household nutrition. RWANU a USAID/Food for Peace Development Food Aid Program in southern Karamoja challenged the cultural norms of male dominated, lucrative, nutrient-rich livestock value chains by engaging women to own and sale livestock, and to also use the income and livestock products for household nutrition.

An assessment of the Women's Livestock Groups was conducted to determine if the program design and outcomes were reached which included: improved women's empowerment, improved nutrition, and improved women's participation in livestock activities.

**Methods:** The assessment used both primary and secondary data sources through a desk review of key programmatic and research documents and through in-depth interviews and focus groups with program beneficiaries, community leaders, key household members, and women not chosen for the program.

**Results:** The assessment showed the program was successful in changing gendered norms around goat ownership, making it socially acceptable for women to own goats and increasing women's sole and joint decision-making power in households. The increase in women's status and value in the household improved along with family relationships. A majority of women reported increased milk consumption especially among small children. On the negative side the men still controlled the selling of the goats, thus control over the money.

**Conclusions:** All three major pathways from livestock (agriculture) to nutrition were affected by this activity: production (home consumption of milk), increased incomes and women's empowerment. Working through women's groups for livestock production allowed the program to explore the social norms and complexities surrounding women's engagement in livestock value chains to improve income and nutrition. However, the assessment found that despite the improvements on improving consumption of milk and changing gender norms around goat ownership, men still maintain primary responsibility for goat care and management, as well as their sale and purchase. Therefore, it is important
tant when designing gender-sensitive livestock projects to include women's economic empowerment activities that will address the social norms that prevent women from engaging and benefiting from their labor.

**Keywords:** livestock, nutrition-sensitive agriculture, women

144/548

ALUMINIUM-CONTAINING FOOD ADDITIVES
EXPOSURE ASSESSMENT

Degrossi, María Claudia; Fattori, Susana.

Department of Chemical Engineering. Buenos Aires Institute of Technology. Argentina.

**Background and objectives:** Available studies show adverse effects of aluminium on the nervous and reproductive systems in animals. The major source of exposure to aluminium for the general population is through the diet. Aluminium in foods originates from its natural occurrence, from the use of aluminium-containing food additives and from the presence of aluminium in food contact materials. The main purpose of this study was to evaluate the presence of food additives containing aluminium in foods, to analyze aluminium content in them and to estimate possible exposure for the general population. JECFA (Joint FAO/WHO Expert Committee on Food Additives) recommended a Provisional Tolerable Weekly Intake (PTWI) of 2mg/kg bw.

**Methods:** A survey was carried out in supermarkets of Ciudad Autónoma de Buenos Aires to determine through the reading of food labels the presence of aluminium-containing food additives authorized for use in food by national legislation. Samples were microwave digested and analyzed by direct graphite furnace atomic absorption spectrometry in order to determine the total aluminium content. Finally, a possible scenario of exposure was established and a weekly potential exposure was estimated.

**Results:** The results of the survey in the supermarkets indicated that flour-based food products as cakes, croissants, rolled cakes, cake powder mixes, among others, are the products that mainly contribute to the ingestion of aluminium. All analyzed products complied with the Food Code limits for food additives containing aluminium (content ranged from less or equal to 0,01 to 0,1g/100g).

On the basis of analytical results, it is possible to infer that the ingestion of one biscuit portion (40g), containing 0,028 g% of aluminium (maximum level detected), 3 times per week, for a 15 kg bw kid represents a potentially weekly intake of 2,2 mg/kg bw, higher than PTWI.

**Conclusions:** Considering that other sources of exposure are possible, and that food industry is using levels of these additives below the maximum allowed amount by national legislation, a deeper risk assessment is required.

**Keywords:** aluminium exposure, risk assessment, food additives, tolerable weekly intake, flour-based food products

144/662

POPULAR RESTAURANT PROGRAM: GUARAN-
TEE OF SAFE MEALS?

(1)Mont'alverne Jucá Seabra, Larissa; (2)Valdejane Silva Souza, Camila; (3)Batista Lima, Luciana.

(1)PhD. Nutritionist. Nutrition Pos graduate program. Federal University of Rio Grande do Norte. Brazil.; (2)PhD. Nutritionist. Nutrition Pos graduate Program. Federal University of Rio Grande do Norte. Brazil; (3)Nutritionist. Federal University of Rio Grande do Norte. Brazil.

**Background and objectives:** The main objective of 'Popular Restaurant Program' is to provide adequate nutritional meals at low cost, to people in risk of food insecurity in Brazil. This work aimed evaluate hygienic-sanitary conditions in Popular Restaurants of Rio Grande do Norte (RN) state.

**Methods:** RN has 24 Popular Restaurants units, and 10 different cities were selected by random sample system. For data collection, a checklist was applied based on Brazilian federal law (RDC 216/2004), containing 183 verification items grouped in 12 evaluation blocks, which allowed the evaluation of the hygienic-sanitary conditions of the establishments. A single visit to the restaurants was carried out to fill out the checklist. To obtain the final diagnosis of each unit, the average of the items in the list was calculated. The restaurants were classified in: "Very bad": 0 to 19% of adequacy of the requirements; "Bad": 20 to 49%; "Regular": 50 to 69%; "Good": 70 to 90% and "Very good": 91 to 100%.

**Results:** The overall adequacy of the 10 Popular Restaurants was 69%, classifying the units as "Regular". The block "building, facilities, equipment, furniture and utensils" was the one that presented the highest percentage of inadequacies. The managers of the visited units reported that the buildings were adapted, and because they were planned for another purposes and not for food production, they did not allow an ordered flow of the food, as well as favored the shelter of vectors and urban pests.

**Conclusions:** The study showed that the establishments presented inadequate conditions for food production and there is no guarantee of safe food to consumers.

**Keywords:** Food Service; Food Safety.

144/710

INTERNATIONALLY COMPARABLE PRICES OF
HEALTHY AND UNHEALTHY FOODS IN 176
COUNTRIES: IMPLICATIONS FOR FOOD AND
NUTRITION POLICIES

Headey, Derek.

PhD Economist. Poverty Health & Nutrition Division.
International Food Policy Research Institute. USA.

**Background and objectives:** While diets typically diversify as incomes rise, decisions on which foods to consume may heavily
depend on relative food prices. Prices will not vary substantially across countries if a food is highly tradable. But while many unhealthy foods are non-perishable and therefore highly tradable (e.g. Coca Cola, oils, salt, sugar), many health foods are highly perishable imperfectly tradable (e.g. eggs, fresh milk). Despite this intuition, no previous research has systematically examined the affordability of different foods across countries.

**Methods:** We use data on the prices of homogeneously defined foods for 176 countries from the 2011 round of the International Comparison Program (ICP). We construct a novel measure of relative prices, the price of 1 kg of a given food ("large brown eggs") relative to the price of 1 kg of the cheapest cereal in each country. This measure captures how expensive it is for households to diversify out of cereals into a specific food group. We compare means and variance of relative food prices across regions, and use least squares regressions to assess cross-country relationships between relative food prices and the consumption patterns of children aged 6-23 months, after controlling for GDP per capita.

**Results:** As expected, relatively unhealthy foods vary little across countries and are often cheap sources of calories. Prices of healthy fruits and vegetables are relatively cheap in developed countries but somewhat more expensive in poorer regions. Animal sourced foods (ASFs) vary substantially across countries and are often very expensive sources of calories, generally 5-15 times more expensive in poor countries compared to OECD countries. Among major food groups it is only the consumption of ASFs that is strongly associated with their own relative prices and with GDP per capita.

**Conclusions:** The expensiveness of healthy foods, particularly ASFs, is a major obstacle to improving diets in poor countries. This suggests that conventional remedies for monotonous diets - income transfers and behavioral change interventions - may be less effective for poor populations facing extremely high ASF prices. This justifies an important role for food policies to drive down the relative prices of nutritious foods.

**Keywords:** Food prices; ASFs; dietary diversity

144/750

EFFECT OF ROSEMARY ON COLORIMETRIC STABILITY OF FLYING FISH HAMBURGER

**Background and objectives:** Obtaining Mechanically Separated Meat (MSM) is an alternative to recovery edible parts of low commercial value fishes. Flying fish (Hirundichthys affinis) constitutes an important fishing resource in the northeast region of Brazil and can be used like raw material in food products. The use of natural compounds as antioxidant has been used to retard undesirable reactions that occur in fish products during storage. Thus, the objective of this work was to evaluate the effect of rosemary (Rosmarinus officinalis) on color stability of 3 different formulations of flying fish hamburger, during freezing storage.

**Methods:** Color determinations were performed on 0, 30, 60 and 90 days of freezing storage with Minolta CM-5 spectrophotometer (CIE L* a* b*). The formulations of hamburger differed in the quantity of rosemary: F1 (0), F2 (0.5%) and F3 (1.0%).

**Results:** The values of lightness (L*) increased throughout the time for all samples at the end of the storage period (p<0.05). Values of b* did not changed throughout the time in F1. However, the samples with rosemary (F2 and F3) had the values of b* increased in the day 90. The values of a* were higher in the samples with rosemary for the analysis carried out in the days 0, 30 and 60, which ensures the increase of the red color intensity in the hamburger, directly proportional to the amount of rosemary in these days of analysis.

**Conclusions:** Formulations with rosemary obtained better color indices during storage, indicating maintenance of the quality when compared to the formulation without rosemary.

**Keywords:** rosemary; fish hamburger; color stability

144/762

FOOD ALLERGEN LABELING AND DETECTION OF TRACES OF MILK, SOY AND EGG IN FOOD PRODUCTS USUALLY CONSUMED BY CHILDREN

**Background and objectives:** Food allergens labeling will soon be incorporated in the Argentine Food Code (article 235 seventh). The objectives of the present study were: to analyze allergen declaration and the presence of precautionary statements in the labels of food usually consumed by children, to quantify traces of milk, soy and egg using commercial ELISA kits in the same products and to evaluate the possible declaration of these allergens according to either Japanese legislation or VITAL 2.0, which establish thresholds in different foods.

**Methods:** Two different batches of 11 commercial products were analyzed, which were randomly acquired in supermarkets in Buenos Aires, Argentina. Correct statements of allergens ac-

<footnote>(1)Suzanne Florentino Silva Chaves Damasceno, Karla; (2)Vanessa Silva Moreira, Camila; (3)Antonio Ponce de Leon F Carvalho, Rodrigo;</footnote>

<footnote>(4)Oliveira Lima, Patrícia; (5)Francisca Marcelino de Paula, Hellen;</footnote>

<footnote>(6)Mont'alverne Jucá Seabra, Larissa.</footnote>

<footnote>(1)PhD. Nutritionist. Nutrition Pos graduate program. Federal University of Rio Grande do Norte. Brazil.; (2)MSc. Nutritionist. Nutrition Postgraduate Program. Federal University of Rio Grande do Norte. Brazil.; (3)PhD. Engineer. Jundiaí Agricultural School. Federal University of Rio Grande do Norte. Brazil.;</footnote>

<footnote>(4)PhD. Zootechnist. Semi-arid Federal Rural University. Brazil.;</footnote>

<footnote>(5)Nutritionist. Federal University of Rio Grande do Norte. Brazil.;</footnote>

<footnote>(6)PhD. Nutritionist. Nutrition Postgraduate Program. Federal University of Rio Grande do Norte. Brazil.</footnote>

<footnote>(1)Ronayne de Ferrer, Patricia Ana; (2)Binaghi, María Julieta;</footnote>

<footnote>(3)Giacomino, María Silvia; (2)López, Laura Beatriz.</footnote>

<footnote>(1)PhD. Biochemist.Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Cátedra de Bromatología. Argentina;</footnote>

<footnote>(2)PhD. Biochemist. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Cátedra de Bromatología. Argentina;</footnote>

<footnote>(3)BsC. Biochemist. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Cátedra de Bromatología. Argentina.</footnote>
cording to article 235 seventh were assessed. Milk, soy and egg allergens were determined using commercial ELISA kits from the brands R-Biopharm, Neogen and/or Romer (two for each allergen).

**Results:** In the case of milk and egg, the kits showed the same behavior in all the analyzed foods and in most of the samples coincided with what was stated on the labels. However, in one of the samples traces of milk were detected even though there was no statement about possible presence of milk in its labeling. The values were 3.9 and 4.2 ppm of milk protein with the R-Biopharm kit and 2.4 and 3.5 ppm of skim milk powder with the Neogen kit. In the analysis of the soy allergen, different results were obtained with the two kits used in some of the samples analyzed. In addition, positive results were obtained for soy in almost all the analyzed samples (9/11 samples), even in 4 samples that did not declare soy at all. In these samples, the values were between 3.6 and 8.7 ppm of soy proteins with R-Biopharm kit. Regarding the Japanese legislation or the VITAL 2.0 system, it is very difficult to conclude what declaration must be made since they are based on different criteria.

**Conclusions:** The final approval of article 235 seventh will be surely a tool of great value for the food industry and allergic patients since the label of any food should provide safe and reliable information for allergic patients and their families.

**Keywords:** Allergens, milk, soy, egg, ELISA

144/773

PHYSICOCHEMICAL QUALITY ASSESSMENT OF
COMMERCIALISED FISH (LUTJANUS JOCU) IN A
BRAZILIAN PUBLIC MARKET

(1)Suzanne Florentino Silva Chaves Damasceno, Karla; (2)Maria Lima Silva, Denise; (2)Patrícia Cabral Ribeiro, Penha; (3)Ucella Dantas Medeiros, Igor; (2)Vanessa Silva Moreira, Camila; (3)Silva Souza, Camila Valdejane; (2)Albert Baracho Rüegg, Rodrigo; (3)Silva Araújo, Samária; (4)Germana Leite, Ana; (5)Mont'alverne Jucá Seabra, Larissa.

(1)PhD. Nutritionist. Nutrition Postgraduate Program. Federal University of Rio Grande do Norte.; (2)MSc. Nutritionist. Nutrition Postgraduate Program. Federal University of Rio Grande do Norte. Brazil.; (3)Nutritionist. Nutrition Postgraduate Program. Federal University of Rio Grande do Norte. Brazil.; (4)Nutritionist. Federal University of Rio Grande do Norte. Brazil.; (5)PhD. Nutritionist. Nutrition Postgraduate Program. Federal University of Rio Grande do Norte. Brazil.

**Background and objectives:** There are several studies that identify health benefits associated with regular consumption of fish and it is important to emphasize that this consumption may be associated with reduced risk of various diseases. However, fishery products are very perishable compared to others of animal origin due fish's own susceptibility for microbial growth and chemical changes. This study aims to assess the physicochemical quality of Lutjanus jocu samples exposed in a Brazilian public market.

**Methods:** The study included 4 lots of fish species Lutjanus jocu, each batch consisting of 5 fish samples. These samples were obtained gutted, headless and without scales on a fish market in the city of Natal/Rio Grande do Norte/Brazil, and subjected to determination of Total Volatile Bases (TVB) and pH analyses.

**Results:** Among the samples analyzed, 75% had TVB values above the limit allowed by current legislation (30 mg N/100g). TVB values ranged from 25.69 to 50.12 mg N/100g and all samples from batch 2 and 4 showed TVB values above the maximum allowed value. Regarding the pH values, 55% of the samples weren't according to the Brazilian legislation. The pH values found were between 5.52 and 7.55.

**Conclusions:** Most fish batches were inadequate in terms of physicochemical quality parameters. Therefore, a risk to the health of the consumer was evidenced, and the forms of manipulation and commercialization should be reviewed in order to control this risk.

**Keywords:** Quality control; fish; physicochemical quality

144/782

UMAMI TASTE PREFERENCE (MONOSODIC GLUTAMATE) IN MEXICAN SCHOLAR CHILDREN

(1)Tinoco-Mar, Briscia Anaid; (2)Valdés Miramontes, Elia Herminia;

(3)Virgen-Carrillo, Carmen Alejandrina; (4)Sánchez-Murguía, Tania Paola.

(1)PhD Student Innovation and Biotechnology, Center for Research and Assistance in Technology and Design of the State of Jalisco, AC (CIATEJ); (2)Ph D. Chemist pharmacobiologist. Centro de Investigaciones en Comportamiento Alimentario y Nutrición. CUSur. University of Guadalajara. Mexico.; (3)BSc. Physician. Centro de Investigaciones en Comportamiento Alimentario y Nutrición. CUSur. University of Guadalajara. Mexico.; (4)MSc. Nutritionist. Centro de Investigaciones en Comportamiento Alimentario y Nutrición. CUSur. University of Guadalajara. Mexico.

**Background and objectives:** Taste is a predictor of food choice. Umami taste is found naturally in food, but monosodium glutamate (MSG) is the main stimulant of umami taste in food. The addition of MSG to food has been popularized being one of the most used and commercialized food additives increases palatability, sensorial qualities and product preference. MSG has specific brain receptors, which block satiety signaling of the consumer, which may contribute to an increase in obesity. In addition, the use of MSG has been associated with adverse side effects such as depression, cardiac arrhythmias and allergies. Research has shown that the taste of MSG is preferred by the entire population, regardless of age, worldwide. The aim of this work was to determine the preference for the umami taste (MSG) in Mexican children.

**Methods:** 120 children (8-11 years) participated, both sexes. The preference for the umami flavor was determined by two sensory evaluation tests: forced double-choice test (2AFC) and analogue visual scale (VAS) range of (0-10), for two types of cracking
one with 0.1% MSG added and another without adding. Statistical analysis was performed by an ANOVA and Chi2 test to determine the preference for a cracking with MSG added or not added.

**Results:** It was determined by VAS that the umami flavor cracker is not rated as preferred by children with a recovery range of 0-3.5 points on the scale; the mean was 2.75 ± .50. 88% of children prefer cracking without added MSG, statistically significant difference. There were no differences by gender or age. The results obtained by 2AFC and VAS are similar to each other.

**Conclusions:** The children who participated in this study prefer a cracking without the addition of MSG and it was determined that the preference for a food with added MSG is low. it should be avoided adding MSG in food because of the adverse effects that its consumption contracts. In Mexico, although some of the industrialized products are highly consumed and contain MSG, children have not generated a taste for the use of this ingredient, so habituation to MSG has not achieved a good percentage of acceptance.

**Keywords:** Preference, umami, children

144/800

POTASSIUM CONTENT IN TEA INFUSIONS

(1)Raimondo, Emilia; (2)Gálvez, Laura; (3)Mezzatesta, Pablo; (2)Flores, Daniel Gustavo; (2)LLaver, Maria Cecilia.

(1)Universidad Juan Agustín Maza. Universidad Nacional de Cuyo;

(2)Universidad Juan Agustín Maza; (3)Universidad Juan Agustín Maza.

**Background and objectives:** The concentration of intracellular potassium is 150 mEq/L and the extracellular concentration of 4-5 mEq/L. There is severe health complications when this balance is broken. In renal patients with glomerular filtration <10% or when potassium intake is multiplied by 20, the potassium balance is altered and it is necessary to control the contribution of the mineral. The aim of the study was to determine the potassium content per cup, for different contact times, types and forms of presentation.

**Methods:** It was worked with 20 types of tea of different brands, in their different presentations: black, green, strands and bags. The infusions were prepared with 2 g of each type of tea and two volumes of hot water: 100 ml and 150 ml, with different contact times: 1, 3, 5 and 7 minutes. It was obtained 280 infusions and it was determined potassium by flame photometry. It was a quantitative and experimental study, in which the variable potassium concentration (dependent) was related to contact time, water volume, type of tea and form of presentation (independent), applying descriptive statistics to the results.

**Results:** It was demonstrated that a cup of 150 ml of tea provided the following amounts of potassium: black (strands) from 63±3 mg to 87±4 mg; black (bags): 75±2 mg to 109±4 mg; green (strands): 80±2 mg to 118±4 mg; green (bags): 68± mg to 93±3 mg; the ranges correspond to different contact times.

**Conclusions:** The results obtained from the potassium content in tea are useful to consider in acute kidney injury, both in

the oliguric phase, where it should not exceed 30-50 mEq/day of the mineral, as in the polyuric phase, where is essential to restore the losses of water and electrolytes. It should be noted that in hemodialysis or dialysis with acidosis and without diuresis, potassium should be restricted to 1 mEq/kg/day to avoid complications. The data obtained provide information that must be taken into account for the calculation of potassium intake.

**Keywords:** Tea, potassium, infusions, kidney disease

**Further collaborators:**

Alejandro Gascón

Gladys Dip

144/995

EVALUATION OF THE MECHANISMS OF MAYONNAISE PHOSPHOLIPID PEROXIDATION

(1)Nakagawa, Kiyotaka; (2)Kato, Shunji; (2)Otoki, Yurika; (2)Ito, Junya;

(3)Miyazawa, Teruo.

(1)PhD. Agriculture. Graduate School of Agricultural Science. Tohoku University. Japan.; (2)PhD. Agriculture. Graduate School of Agricultural Science. Tohoku University. Japan; (3)PhD. Agriculture. New Industry Creation Hatchery Center (NICHe). Tohoku University. Japan.

**Background and objectives:** Mayonnaise, which is widely used in foods, is rich in lipids and therefore susceptible to oxidation during the manufacturing process, which can result in loss of quality. Hence, an improved understanding of the cause of mayonnaise lipid peroxidation could lead to a means of ensuring maintenance of the quality of the product.

Several classes of lipid hydroperoxides may be present in mayonnaise, such as triacylglycerol hydroperoxide, cholesterol hydroperoxide and phospholipid hydroperoxide. Of these lipid hydroperoxides, we have recently developed a sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for detecting phosphatidylcholine hydroperoxide (PCOOH), a primary oxidation product of phosphatidylcholine (PC). Determination of the hydroperoxide group position in PCOOH isomers may be helpful for elucidating the cause of mayonnaise lipid peroxidation, as demonstrated in our previous study (Anal. Biochem., 471, 51-60, 2015) in which we analyzed PCOOH isomers in human plasma and found that radical (and/or enzymatic) oxidation, rather than singlet oxygen-oxidation, is likely the cause of plasma PC peroxidation.

**Methods:** Based on above findings, we analyzed PCOOH isomers in 3 commercial mayonnaise using LC-MS/MS in the present study.

**Results:** PCOOH analysis was carried out with 3 commercial mayonnaise products immediately after unsealing. From the analysis, it was estimated that about 0.01-0.03% of PC had already been oxidized to hydroperoxide prior to opening. To the best of our knowledge, this is the first report demonstrating that oxidized phospholipid was detected in mayonnaise, despite of the freshness. The PCOOH isomer composition suggests that mayonnaise phos-
pholipid peroxidation is predominantly initiated by radical-oxidation (i.e. upon autoxidation), rather than singlet oxygen-oxidation (e.g. upon light exposure), during manufacturing, packaging and/or storage. Hence, antioxidants such as tocopherol (radical trapping reagent), rather than carotenoids (singlet oxygen trapping reagent), can be more useful to prevent mayonnaise phospholipid oxidation.

**Conclusions:** The present LC-MS/MS method will be useful for elucidating the cause of lipid peroxidation in mayonnaise and related foods. Such information will be valuable to ensure maintenance of product quality.

**Keywords:** Mayonnaise, Oxidation, Phospholipid, Hydroperoxide isomer, LC-MS/MS

**Results:** Our results showed that parboiling decreased the arsenic contents in the edible portion of Akamoku by about 85% by processing for food (Murakami et al., 2013, ISUN 20th International Congress of Nutrition. PO3182). Further, we report changes in arsenic contents in the different organs of Akamoku after parboiling.

**Conclusions:** The biochemical mechanism of the arsenic accumulation in high level in the intact plants are yet obscure, a proper treatment to the plants will lead to a good foodstuff to supply dietary fiber and minerals.

**Keywords:** Sargassum horneri, Akamoku, arsenic, seaweed, brown alga

**Further collaborators:**

This work was supported by grant of Fukutake Foundation.

## CHANGES IN ARSENIC CONTENT IN THE DIFFERENT ORGANS OF SEAWEED: AKAMOKU, SARGASSUM HORNERI, AFTER PARBOILING

(1)Murakami, Kaori; (2)Sugawa-Katayama, Yohko; (2)Katayama, Masayuki.

(1)Associate Professor. Department of Food Sciences and Biotechnology. Faculty of Life Sciences. Hiroshima Institute of Technology. Japan.; (2)Professor. Department of Health and Nutrition, Osaka Aoyama University.

**Background and objectives:** Akamoku, Sargassum horneri Turner C. Agardh, is an annual seaweed that grows along ocean coasts, and is considered a good foodstuff consisting as it contains high amounts of dietary fiber and minerals (Murakami et al., 2016, Natural Resources). This seaweed is native to Asia with a wide distribution range from the Japanese coast to the East and the South China Seas. However, this seaweed was observed in California, USA and Baja California, Mexico in recent years. Similar to the other members of Phaeophyta, Akamoku contains a rather high level of arsenic (Katayama et al., 2008, Trace Nutrient Research). Akamoku has been used as food only in a few areas of Niigata and Akita Prefectures, Japan, but recently, Toyama, Iwate, and Fukuoka Prefectures have begun using it as a food material. We measured the arsenic accumulation in Akamoku in order to gain a better perspective on the species' safety and usability as a seafood product. Further, we attempted to understand the arsenic accumulation behavior in different part of the seaweed for its better utilization as a foodstuff.

**Methods:** Akamoku plants were harvested in March 2017 at Suo-oshima, Inland Sea, Seto, Japan. Mature male and female plants were harvested separately, washed thoroughly, and separated into inedible and edible portions; receptacles, branch (with leaves and air-vesicle), and stalks. Half of edible portion was parboiled with 2.2%NaCl for 1 min. The boiled sample was then refrigerated with ultrapure water for 1 min. The total arsenic and inorganic arsenic contents were determined by atomic absorption spectrophotometry and high performance liquid chromatography with inductively coupled plasma mass spectrometry.

## EDIBLE PROTEIN ENERGY RETURN OF INVESTMENT (EP-EROI) AS A TOOL TO ECO-NUTRIENT COMPARISON OF CANNED SEAFOOD PRODUCTS

(1)Aldaco García, Rubén; (2)Laso, Jara; (3)Margallo, María; (4)Vazquez-Rowe, Ian.

(1)Associate Professor. Department of Chemical and Biomolecular Engineering. Cantabria University. Spain.; (2)PhD student. Department of Chemical and Biomolecular Engineering. Cantabria University. Spain.; (3)Assistant Professor. Department of Chemical and Biomolecular Engineering. Cantabria University. Spain.; (4)Associate Professor. Pontificia Universidad Católica del Perú. Departamento de Ingeniería. Red Peruana Ciclo de Vida. Peru.

**Background and objectives:** Each year approximately 1.3 billion tons of edible food are wasted throughout global food supply chain generating emissions of 3.3 Gt CO2 eq. This value will be increased due to the global population is expected to increase from the current 7 billion to nearly 10 billion by 2050. These facts make that current patterns of food production and consumption are increasingly considered to be unsustainable, i.e. the amount of energy used to produce, process, package, store, and transport food is seven and half times the amount of energy the food actually provides in return. In addition to cause environmental impact, wasting food involves the loss of nutrients along the food supply. The cooking way and eating habits can vary the nutrient content of food, because up to 42% of food waste is produced by households. In this context, the European Commission is conducting several improvement measures to reduce food loss and waste by 50% by 2020. The Bioeconomy Strategy aims to pave the way to a more innovative, resource efficient and competitive society that reconciles food security with the sustainable use of renewable resources while ensuring environmental protection. On the other hand, Food 2030 initiative have four priorities: (i) nutrition for sustainable and healthy diets, (ii) climate smart and environmentally sustainable food systems, (iii) circularity and resource effi-
ciency of food systems, and (iv) innovation and empowerment of communities.

**Methods:** In this framework, this work develops a methodological approach, which assess and compares both the environmental impact of a food product and its energy content returned to consumer. The environmental impact is evaluated with the standardized methodology, life cycle assessment (LCA). On the other hand, the nutrient properties of the product are analyzed by means of the protein content along the life cycle chain.

**Results:** Both metrics can be expressed in terms of energy consumption (Cumulative Energy Demand) and energy content (protein content) by means of the Edible Protein Energy Return of Investment (Ep-EROI).

**Conclusions:** This methodology can be applied to all food chain products; however, this paper is focused on canned anchovy products.

**Keywords:** European anchovy; protein content; environmental impact; food supply chain.

the plant tissues were determined by atomic absorption spectroscopy.

**Results:** The concentration of calcium (Ca) per tissue weight increased gradually coming to a rather constant value before the appearance of genital organs on the plants, although the concentration of arsenic (As) continued to increase. Until that time, the concentrations of zinc (Zn) and manganese (Mn) continued to increase further. The concentration of iron (Fe) and magnesium (Mg) increased gradually, expressing a strong correlation between them, but the concentration of magnesium (Mg) was not uniform in the chlorotic plants.

**Conclusions:** From the viewpoint of nutritional value, matured plants of Hijiki had better be harvested, but to keep good quality of the products, fine days under the sunshine should be selected, even before complete maturation.

**Keywords:** Hijiki, Sargassum fusiforme, mineral accumulation, growing process.

144/1033

## ACCUMULATION PROCESSES OF NUTRITIONAL-LY BENEFICIAL MINERALS IN A BROWN ALGAE, HIJIKI (SARGASSUM FUSIFORME), DURING GROWTHFUSIFORME), DURING GROWTH

(1)Katayama, Masayuki; (2)Sugawa-Katayama, Yohko; (3)Murakami, Kaori.

(1)PhD. Professor. Department of Health and Nutrition. Osaka Aoyama University. Japan.; (2)PhD. Professor. Department of Health and Nutrition. Osaka Aoyama University. Japan;

(3)PhD. Associate Professor. Department of Food Sciences and Biotechnology. Hiroshima Institute of Technology. Japan.

**Background and objectives:** The brown algae Hijiki, Sargassum fusiforme, is an important foodstuff for Japanese, and serves as a rich supply of minerals and dietary fiber. Growing of the earth elevates the ocean-water temperature year by year, and exerts ecological influences. Hijiki also seems to change their regular growing processes, becoming chlorotic earlier than before. To know the physiological changes during their growth, we studied the accumulating processes of several nutritionally beneficial minerals in Hijiki plants during their growth from the primary leaf stage to their chlorotic state. Although the accumulation of arsenic (As) occurred in plants during their growth, arsenic (As) could be mostly removed during the pre-cooking process of the commercial dried Hijiki products.

**Methods:** Hijiki plants were harvested at the seashore of Kusahimoto, Wakayama Prefecture, Japan. The fresh samples of Hijiki plants were lyophilized and decomposed with conc perchloric acid - conc nitric acid, or with conc sulfuric acid - conc nitric acid. The ashed samples were dissolved in 1N hydrochloric acid, and the concentrations of arsenic (As), calcium (Ca), iron (Fe), magnesium (Mg), manganese (Mn), potassium (K), and zinc (Zn) in

144/1038

## FOOD LOSSES AND FOOD SECURITY AND NUTRITION: ESTIMATING THE POTENTIAL OF A RESPONSIBLE FOOD PRODUCTION AND CONSUMPTION

(1)Aldaco García, Rubén; (2)García-Herrero, Isabel; (3)Fullana, Pere;

(4)Bala, Alba; (5)Laso, Jara; (6)Vazquez-Rowe, Ian; (7)Durá, María José;

(8)Abajo, Rebeca; (8)Sarabia, Carmen; (9)Gazulla, Cristina.

(1)Associate Professor. Department of Chemical and Biomolecular Engineering. Cantabria University. Spain.; (2)Researcher. Department of Chemical and Biomolecular Engineering. Cantabria University. Spain; (3)Professor. UNESCO Chair in Life Cycle and Climate Change. Escola Superior de Comerç International (ESCI), Universitat Pompeu Fabra (UPF). Spain;

(4)Researcher. UNESCO Chair in Life Cycle and Climate Change. Escola Superior de Comerç International (ESCI), Universitat Pompeu Fabra (UPF). Spain; (5)PhD student. Department of Chemical and Biomolecular Engineering. Cantabria University. Spain; (6)Researcher. Pontificia Universidad Católica del Perú. Departamento de Ingeniería. Red Peruana Ciclo de Vida. Perú;

(7)Associate Professor. Nursing Department. Cantabria University. Spain; (8)Assistant Professor. Nursing Department. Cantabria University. Spain; (9)Researcher. Lavola Cosostenibility. Barcelona. Spain.

**Background and objectives:** Food and nutrition security is a complex concern, related to health through malnutrition, but also to sustainable economic development, environment, and trade. Europe and Central Asia as a region has achieved the Millennium Development Goal hunger target of reducing by half the proportion of people affected by hunger. At the same time, large amounts of food are being wasted, food that still may contain important nutrients and micronutrients. An estimation of macronutrient and micronutrient loss resulting from food waste may be helpful for people trying to prevent food waste by engaging the public and
companies and increasing awareness on this subject. Estimation of the impact of food waste on health and nutritional issues can be performed by calculation of nutrient losses resulting from food waste.

**Methods:** A comparison of loss of nutrients by losing and wasting food, with the recommended daily intake (RDI) of that specific nutrient or micronutrient has been calculated in this work. Two parameters are needed to perform such a calculation: i. amounts of wasted products, and ii. nutritional data describing the macronutrient and micronutrient composition.

**Results:** Taking as a case study the food losses in Spain, preliminary results shows that the estimated amount of vitamin C that is lost in Spain in a year as a result of food waste may contain the equivalent of 1.9 billion "recommended daily intake portions" which is equivalent to the amount of vitamin C that is needed by 5.3 million people a day.

**Conclusions:** Reducing food waste has enormous potential for reducing the resources we use to produce the food we eat. Being more efficient will save money and lower the environmental impact of food production and consumption, contributing to a global nutrition security.

**Keywords:** Food loses; food security; environmental friendly nutrition

**Further collaborators:**

María Margallo. Assistant Professor. Department of Chemical and Biomolecular Engineering. Cantabria University. Spain.

Angel Irabien. Professor. Department of Chemical and Biomolecular Engineering. Cantabria University. Spain.

144/1039

NON-DESTRUCTIVE RIPENING INDEXES OF
PRICKLY PEAR (OPUNTIA FICUS-INDICA) FOR
AN OPTIMAL FREEZE-DRYING

(1)Ramírez Anaya, Jessica del Pilar; (2)Castañeda-Saucedo, María Claudia; (3)Martín del Campo Barba, Sandra Teresita; (4)Gómez Hernández, Hector Eduardo; (5)Mendez Morán, Lucila.

(1)PhD. Food Tecnhologist. Universidad de Guadalaja, Centro Universitario del Sur, Departamento de Desarrollo Regional;

(2)PhD. Agricultural studyes. Depto. Sociedad y Economía. Universidad de Guadalajara. Centro Universitario del Sur. Ciudad Guzmán. México.; (3)PhD. Food Technolysts. Departamento de Industrias Alimentarias. Instituto Tecnológico y de Estudios Superiores de Monterrey. Querétaro. México.; (4)PhD. Food Technologist. Depto. de Ingeniería. Química. Universidad de Guadalajara. Centro Universitario de Ciencias Exactas e Ingenierías.; (5)PhD. Genetics. Departamento de Ecología. Universidad de Guadalajara. Centro Universitario de Ciencias Biológicas.

**Background and objectives:** Prickly pears (Opuntia ficus-indica va. Burrona) are climacteric fruits and usually are marketed in fresh. Freeze-dry can be used to obtain innovative foods with higher added value that can help to improve the Mexican economy

of semiarid zones. It is known that some variations in raw material characteristics determined the quality in the final product. Our aim was to non-destructively select raw material with the optimal ripening conditions to freeze-dry prickly pear slices in order to obtain the best-rehydrated products. By accumulated growths curves, the ripening index was determined during pre-harvest of 300 fruits that were obtained from the central Mexican zones.

**Methods:** Each week, the change of color, texture, acidity, pH, chlorophyll content, reducing and total sugars content were evaluated. The fruits were selected by their peel color using a* parameter, for freeze-drying experiments under laboratory conditions. ANOVA (a=0.05) was used to determine the effect of two slice thickness (1 and 1.5 cm) and two plate temperatures (15 and 30oC), in relation with; pulp drying time, rehydration coefficients of freeze-dried products, color and texture of rehydrated products.

**Results:** The fruit ripening process began on day 90 after full bloom, where the soluble solids/tritiable acidity ratio (r=0.75) as well as fruit texture (r=0.78) correlated in a positive way with a*, making it possible to differentiate fruits in the different ripening stages. The fruits with a* value of -7.6± 0.43 (111 days after flowering) were selected as an optimal raw material for freeze-drying. Drying time was significantly reduced in a 1 cm slice thickness and 30°C of plate temperature. These results compared with fresh slices, showed that dried slices had higher L* and a* intensity. However, the rehydration coefficient was low with a loss on texture, as well as reduction on L* and a* after rehydration.

**Conclusions:** Fruit peel color makes it possible to non-destructively differentiate fruits during ripening. Freeze-dried slices of prickly pears are a healthy snack and are a marketing option, due to do not show signs of retraction and their flavor is preserved.

**Keywords:** Freeze-dry, prickly pear, ripening index, rehydration, color

144/1069

DETERMINATION OF MAGNESIUM IN SPINACH
UNDER DIFFERENT COOKING METHODS

(1)Raimondo, Emilia; (2)Farah, Silvia; (3)Dip, Gladys; (3)Gascón, Alejandro.

(1)Universidad Juan Agustín Maza- Universidad Nacional de Cuyo;

(2)Universidad Juan Agustín Maza; (3)Universidad Nacional de Cuyo.

**Background and objectives:** Magnesium is necessary for about 300 biochemical reactions of the body, with a requirement of 260 mg/d for a healthy adult. Therefore the aim of the study was to determine how the content of magnesium in spinach varies under different cooking processes.

**Methods:** To carry out this experience it was used 10 kg of spinach, acquired and processed on the same day. For the sampling, each plant was divided into 5 equal parts, obtaining 5 lots submitted to different cooking techniques: Lot 1: raw spinach, which was reserved for analysis; Lot 2: spinach cooked by boiling; Lot 3: sauteed spinach; Lot 4: steamed spinach; Lot 5: spinach
cooked using microwave. It was determined the centesimal com-
position of raw and cooked product applying official laboratory
techniques. The magnesium content was determined by atomic
absorption spectrometry on ash dilution. In order to establish if
there is statistically significant differences. It was applied analysis
of variance (ANOVA) for a level of significance alpha ≤0.05.

**Results:** The loss of humidity was statistically significant for sauteed spinach (73 g%) and cooked with microwave (60 g%). The fat content is increased from 0.3 g to 12.58 g in sauteed spinach, which represented a 5.4 times more caloric intake. The magnesium content was 64±3 mg% in raw spinach; by boiling 52±2 mg%; sauteed 86±3 mg%; steamed 63±3 mg% and microwave 99±4 mg%.

**Conclusions:** Spinach processed with microwaves presented the greatest loss of weight and volume (-70%), but concentrated in a 50% the magnesium content, in relation with the loss of humidity. The one cooked by boiling had the greatest loss of magnesium (leaching of minerals). Steamed spinach maintained the content of magnesium. The sauteed one had a loss of weight and volume of 18%, and also it concentrated 35% magnesium respect to raw spinach, but increased its caloric value.

**Keywords:** Magnesium, cooking techniques, nutritional com-
position, caloric intake.

**Further collaborators:**

Luisina Capone, Gisel Maimone, Romina Sosa

Retinol was analysed through HPLC and Ascorbic Acid by capillary electrophoresis.

**Results:** Retinol and Ascorbic Acid were detected in cooked and enriched samples, and consequently pre and post-cooking samples were analysed.

Retinol increased its concentration in post-cooking samples
due to water loss secondary to evaporation (11% between non-en-
riched, 14% among enriched). Between cooked samples, enriched
sample presented an 18% increase versus non-enriched one,
whereas among raw samples it was 15%.

Ascorbic acid content increased due to enriched cereal addition.
Cooking generated a partial loss (less than 15%). In all samples, var-
iations were related to initial concentrations and cooking methods
that included different heat transfer and O2 partial pressure.

Vitamin loss is partial and predictable, obeying to a natural
chemical behaviour rather than the procedure itself: solutes with
higher concentrations are the most affected by changes in environ-
ment conditions.

**Conclusions:** Complementary feeding is one of the critical moments for micronutrients intake. Partial and predictable vita- min losses in homemade preparations turn micronutrients en- riched cereal into a useful tool to fortify children's meals, ensuring an adequate micronutrients supply.

**Keywords:** Retinol, Ascorbic Acid, complementary feeding, homemade meals

**Conflict of Interest Disclosure:** The study was funded by Danone Nutricia ELN.

**Further collaborators:**

Yazmín Iaquinta. Universidad Maimónides. Buenos Aires. Ar-
gentina.

**Background and objectives:** Nutrition during childhood is key for diseases prevention in adult life. Complementary feeding carries the risk of micronutrients inadequate intake.

Homemade meals elaboration implies vitamins losses in differ-
ent cooking procedures. Using enriched weaning food would cov-
er nutrient recommendations. The study aims to evaluate Retinol
and Ascorbic Acid's concentrations variation secondary to cook-
ing procedures, on meals intended for children, using micronutri-
ents enriched infant cereal as an ingredient.

**Methods:** Exploratory experimental study analysed Ascorbic Acid and Retinol in two recipes with different cooking procedures. These vitamins were selected as indicators of lability between hy- drosoluble and liposoluble vitamins. The recipes were elaborated as homemade meals under replicable conditions.

**Background and objectives:** The causal pathway between food production, consumption, knowledge and improved nutrition outcomes is neither direct nor simple and depends on a complex interaction of a range of factors. The Harnessing Agriculture for Nutrition Outcomes (HANO) project was designed to explore the linkages between household agricultural production with dietary patterns and nutrition. The project was implemented from

<footnote>(1)Kabbache, Diana Miriam; (1)Batista, Mariana; (2)Ketelhohn, Belen;</footnote>

<footnote>(3)Olagnero, Gabriela.</footnote>

<footnote>(1)Nutritionist (BSc). Técnica Dietoterápica. Universidad de Buenos Aires. Universidad del Salvador. Buenos Aires. Argentina;</footnote>

<footnote>(2)Nutritionist (BSc). Scientific Coordination. Research and Development. Danone Nutricia. Buenos Aires. Argentina;</footnote>

<footnote>(3)Nutritionist (BSc). Scientific Coordination. Research and Development. Danone Nutricia. Buenos Aires. Argentina.Buenos Aires.</footnote>

<footnote>(1)Roschnik, Natalie; (2)Kivuyo, Nengilang'et G.; (3)Muruve, Joanita Aigi; (4)Ngombalu, Assumpta Ndumi Ngombalu Ngombalu;</footnote>

<footnote>(5)Copestake, James; (6)Remnant, Fiona.</footnote>

<footnote>(1)MSc. Senior Research and Impact Advisor. Save the Children. UK; (2)MSc. Regional Programme Manager. Save the Children. Tanzania; (3)MPH. Deputy Director Program Quality and Development. Save the Children. Tanzania; (4)MPH. Nutrition Adviser. Save the Children. Kenya; (5)PhD. Professor of International Development. Centre for Development Studies. University of Bath. UK; (6)MSc. Director. Bath Social and Development Research Ltd. UK.</footnote>
December 2012-present in Lindi and Ruangwa districts in Lindi region Tanzania, with an aim to prevent chronic malnutrition among children under two years by combining nutrition interventions with a range of agricultural activities.

**Methods:** To assess the project's impact, an end line evaluation will be undertaken at the end of the project in May 2017 using the Qualitative Impact Protocol (QuIP). Data will be collected from a sample of the intervention villages using semi-structured interviews with 30 households and 4 focus groups - using blindfolded, independent researchers. The questionnaire makes no reference to the project, but is designed to capture information on wellbeing domains expected to have been impacted according to the project's theory of change. The narrative data collected is then analysed against the project's theory of change to identify unexpected as well as anticipated drivers of change, and the interaction of the intervention with these drivers.

**Results:** QuIP sets out to generate differentiated evidence of impact based on narrative causal statements elicited directly from project beneficiaries. Evidence of attribution is sought through respondents' own accounts of causal mechanisms rather than by relying on statistical inference based on variable exposure. Data is presented according to whether it a) explicitly attributes impact to project activities, (b) makes statements that are implicitly consistent with the project's theory of change, (c) refers to drivers of change that are incidental to project activities. This data can then be triangulated with routine monitoring data in 'unblindfolding' workshops, assessing what the findings mean for future project design.

**Conclusions:** We hope that conducting a 'deep-dive' using the QuIP methodology will help to provide much richer information on the causal impacts of different activities and drivers of change in Lindi and Ruangwa districts. The results of the evaluation will be presented.

**Keywords:** agriculture, nutrition, QuIP, evaluation

**Further collaborators:**

Acknowledgements: This project was funded by Irish Aid

lations such as young children and women of reproductive age in low- and middle-income countries. Biofortified beans with substantial concentrations of iron and zinc were introduced in Colombia in 2016. These beans have the potential to reach vulnerable populations and to improve their nutritional statuses, considering beans are staple crops and frequently consumed in Colombia. Successful implementation however, depends on the acceptability of these newly bred varieties. Therefore we studied the sensory acceptability of two biofortified bean varieties high in iron and zinc and a commonly eaten local bean variety.

**Methods:** Two biofortified bush-beans varieties known as BIO-101 and BIO-107 were included, with iron and zinc concentrations between 8.2-8.3 mg/100g and 4.3-4.4 mg/100g, respectively. Schoolchildren (n=174), aged between 10-15 years old, from (semi-) rural areas in the departments of Cauca and Valle del Cauca were asked to evaluate these varieties and a local bean variety. The three bean varieties were presented as blind-labeled samples of 20 grams and assessed on color, size, smell, taste and texture using a five-point facial likert scale.

**Results:** The overall mean scores were 3.88 ± 0.64, 3.79 ± 0.74 and 3.81 ± 0.76, for BIO-101, BIO-107 and the control sample respectively and these scores did not significantly differ from each other (p>0.05). The children in Cauca (n=61) slightly preferred BIO-107 over the control sample (p <0.05), but not over BIO-101, based on color, smell and taste. The children in Valle del Cauca (n=113) slightly favored the control sample over BIO-107 (p<0.05), but not over BIO-101, regarding size, smell and taste.

**Conclusions:** Overall, all varieties are well and equally accepted, which seem to have promising implications for the implementation and impact of biofortified iron/zinc beans.

**Keywords:** Biofortification; Beans; Micronutrients; Sensory acceptability; Hedonic

144/1213

THE SENSORY ACCEPTABILITY OF TWO BIO-FORTIFIED IRON/ZINC BEAN VARIETIES BY PRE-TEENS FROM RURAL- AND SEMI-RURAL AREAS IN THE ANDEAN REGION OF COLOMBIA

(1)Beintema, Joni Jade Serena; (2)Gallego-Castillo, Sonia; (3)Restrepo-Manjarres, José; (4)Talsma, Elise Francina.

(1)MSc. Nutritionist. Harvestplus, International Centre for Tropical Agriculture (CIAT). Colombia; (2)BSc. Chemical engineer. Harvestplus. International Centre for Tropical Agriculture (CIAT). Colombia; (3)MSc. Agronomist. Foundation for Agricultural Research and Development (FIDAR). Colombia; (4)PhD. Nutritionist. Harvestplus. International Centre for Tropical Agriculture (CIAT). Colombia.

**Background and objectives:** Iron and zinc deficiency are mayor public health problems, mostly affecting vulnerable popu-

144/1249

LABELING AND REPORTING OF SALT/SODIUM IN PACKAGED FOODS

(1)Singh, Meenu; (2)Chandorkar, Suneeta.

(1)Post Doctoral Fellow. Division of Nutrition. St. John's Research Institute. St. John's National Academy of Health Sciences. Bangalore 560034. Karnataka. India.; (2)Assistant Professor. Department of Foods and Nutrition. Faculty of Family and Community Sciences. The Maharaja Sayajirao University of Baroda. Vadodara 390002. Gujarat. India.

**Background and objectives:** Packaged foods report salt in the ingredients list and sodium content in nutrition facts panel. Information on salt and/or sodium content can be used to select low sodium foods to reduce the risk of hypertension and resultant coronary heart diseases. Therefore, the aim of the present investigation was to evaluate the accuracy and reliability in reporting of salt/sodium content on labels of packaged foods.

**Methods:** A total of 1,020 packaged foods (29=food categories, 10=food groups) were surveyed from supermarkets (n=4)
and grocery-stores (n=4) in Vadodara, Gujarat, India and the labels were studied for compliance with the legislations laid down by Food Safety and Standards Authority of India (FSSAI). One hundred and fifty four packaged foods were analyzed for sodium content by AOAC 969.23 method using Flame Photometer (Mod el: Elico CL 361).

**Results:** Results revealed that 11% of the products had multi-ple and alternative sources of salt/sodium (common salt, rock salt, sodium-carbonate/caseinate/molybdate, monosodium glutamate) in ingredients list, making them high in sodium content. Ready-to-cook/eat products had the highest percentage (4.4%) of products listing multiple sources of salt followed by snacks (2.9%), wheat and oats based products (1%), bakery products (0.9%), confectionery (0.5%), food adjuncts (0.3%), milk based products (0.1%). When MSG (a source of sodium) and salt were considered together, ready to cook/eat products were found to have highest percentage (4.5%) of Salt+MSG. Four percent of the products had multiple sources of MSG (namely, hydrolyzed-vegetable protein/corn solids, yeast extract, etc). Multiple MSG sources were highest in ready-to-cook/eat products (2.1%) followed by wheat and oats based products (1%), snacks (0.6%) and food adjuncts (0.2%). Ingredient claims like "no added salt" and "No MSG" were not substantiated by any of the product's ingredients list. Products with higher analyzed values for sodium than those reported on the label were found to have multi-ple/alternate sources of sodium in the ingredients list.

**Conclusions:** There is lack of accuracy and reliability in reporting of salt/sodium and MSG information on packaged food labels. There is a need to improve compliance with legislation for labeling of packaged foods laid down by FSSAI for improved Public Health and Nutrition outcomes.

**Keywords:** Salt, Sodium, Mono-sodium glutamate (MSG), Food Labeling, Packaged Foods.

**Further collaborators:**

Meenu Singh and Suneeta Chandorkar

ation diet exceeds the recommendation for TFA, by high consume of processed food. According to Resolution RDC 360/2003 of the National Health Surveillance Agency/Ministry of Health, it is required the grade of TFA on labeling of packaged foods, this is a strategy for chronic diseases prevention. The World Health Organization warned of the need of less TFA consume, which culminated in the recommendation for its elimination in 2004. The result of this control will be the improvement of the health of the population. Considering the legal requirements and the consumer's right to obtain reliable information, the objective is to address aspects about TFA, emphasizing food labeling and Brazilian legislation.

**Methods:** SciELO and Lilacs databases were used, with the terms "trans fatty acids" and "hydrogenated fatty" alone or in conjugated with "labeling", "regulation" or "legislation". The search was from 2003 to 2017, were included original articles and excluded researchers outside Brazil or reviews, were selected 10 originals articles.

**Results:** Several categories of industrialized products were evaluated for the TFA content and their labeling. Among them are snacks, fast foods, pasta, cereals, dairy products, infant formulations, vegetable oils, among others. All the articles analyzed have concluded that industrialized product labels require greater scrutiny and the need to revise national legislation on the amount of TFA to be declared. The TFA content is higher in 56% of the analyzed articles compared to the declared on the label and exceeds the limit allowed by the legislation.

**Conclusions:** After 14 years of implementation of the legislation in Brazil, the indication of TFA on labels and their use by the industry are still inadequate. Need for revision of the legislation in the indication of the content of TFA in the label and change of the nutritional information for a percentage of the food instead of the portion in grams. Increased vigor in the regulation and monitoring of nutrition labeling will be beneficial to consumer health, thus reducing the risk of developing chronic diseases and public health expenditures.

**Keywords:** Trans fatty acids. Industrialized foods. Legislation. Nutritional labeling. Public healthy

144/1273

TRANS FATTY ACIDS: AFTER 14 YEARS OF BRAZILIAN LEGISLATION ARE THE LABELING ADEQUATED?

**Background and objectives:** The excessive intake of trans fatty acids (TFA) has been related to the development of cardiovascular diseases. Recent researches show the Brazilian popula-

144/1286

DEVELOPMENT OF NUTRITIONAL AND HEALTHY BAKERY PRODUCTS BY INCORPORATION OF QUINOA

**Background and objectives:** Today, nutrition is located as part of the prevention or reduction of certain diseases. The ob-

<footnote>(1)Mafioletti, Marina; (2)Yoshime, Luciana; (3)Sattler, José Augusto;</footnote>

<footnote>(4)Corsi, Giovanna; (2)Melo, Illana Louise; (5)Mancini-Filho, Jorge.</footnote>

<footnote>(1)Sl. Graduate student of Nutrition. Faculty of Pharmaceutical Sciences. University of Sao Paulo. Brazil; (2)PhD. Nutritionist. Faculty of Pharmaceutical Sciences. University of Sao Paulo. Brazil.; (3)PhD Candidate. Nutritionist. Faculty of Pharmaceutical Sciences. University of Sao Paulo. Brazil.; (4)Sl. Graduate Student of Nutrition. Faculty of Pharmaceutical Sciences. University of Sao Paulo. Brazil.; (5)PhD. Senior Professor. Faculty of Pharmaceutical Sciences. University of Sao Paulo. Brazil.</footnote>

<footnote>(1)Haros, Claudia Monika; (2)Fernández-Espinar, María Teresa; (3)Gil, José Vicente.</footnote>

<footnote>(1)PhD. Chemist. Cereal Group. Institute of Agrochemistry and Food Technology (IATA CSIC). Valencia. Spain.; (2)PhD. Biologist. Biological Activity of Food Compounds. Institute of Agrochemistry and Food Technology (IATA CSIC). Valencia. Spain.; (3)PhD. Biologist. Departamento de Medicina Preventiva y Salud Pública, Ciencia de Alimentos - Toxicología y Medicina Forense. University of Valencia. Spain.</footnote>
taining and characterizing new ingredients with high technological potential, high nutritional value and bioactive compounds, opens up a promising field of competitiveness to the food industry with which to be able to establish itself in the food market. Consumption of whole grain or wholemeal can play an important physiological role in maintaining general well-being and health, not only because of its fibre content but also because it contains numerous bioactive components. Quinoa grains (Chenopodium quinoa) are characterized by a high concentration of proteins with excellent amino acid profile in addition to a high content of minerals, vitamins, unsaturated fatty acids and antioxidants. The objective of this research has been to develop new bakery products by replacing flour with whole quinoa flour from Bolivia (white, red and black varieties), to evaluate its functionality as a bakery ingredient.

**Methods:** The nutritional, technological and sensorial quality of the developed products was determined in terms of moisture, starch, proteins, dietary fibre, ash, polyphenols, colour parameters and sensorial analysis.

**Results:** The incorporation of quinoa increased significantly the content of dietary fibre (soluble and insoluble), proteins, lipids and minerals. The colour of the crust and crumb was significantly modified with quinoa variety in comparison to control sample. Polyphenolic profile by HPLC showed the presence of more polyphenol classes in quinoa flours than in wheat flour. Their inclusion produced breads with increased polyphenol content and DPPH radical scavenging activity always in levels significantly higher compared to the control. The sensory analysis indicated that the substitution of 25% of flour for quinoa did not significantly affect the overall acceptability of bread, with slightly better scored products with quinoa than control sample.

**Conclusions:** Whole quinoa flour could be a good replacement for wheat flour in bread formulations, increasing the product's nutritional value and bioactive compounds with only a small depreciation in bread quality at 25g/100 g of flour substitution. The inclusion of quinoa flours had a positive effect on the technological and sensorial value of the bread products, and therefore its inclusion is recommended.

**Keywords:** quinoa, bakery products, dietary fibre, polyphenols, minerals

**Further collaborators:**

This work was financially supported by grants AGL2016-75687-C2-1-R from the Ministry of Economy, Industry and Competitiveness.

## REDUCTION OF SODIUM CONTENT, INCREASE OF DIETARY FIBER AND YEAST, AND NUTRITIONAL INFORMATION: DO THEY AFFECT THE ACCEPTABILITY OF OPTIMIZED BREAD REGARDING CONSUMERS IN BUENOS AIRES, ARGENTINA?

(1)Watson, Dana Zoe; (1)Hernández, María; (2)Lema, Silvia; (2)Vázquez, Marisa Beatriz.

(1)BSc. Nutritionist. Escuela de Nutrición. Universidad de Buenos Aires. Argentina.; (2)PhD. Nutritionist. Escuela de Nutrición. Universidad de Buenos Aires. Argentina.

**Background and objectives:** A reduction in salt (NaCl) intake and an increase in fiber promote the prevention of cardiovascular diseases. In Argentina, the daily salt intake is higher and dietary fiber is lower than the recommendations. Bread is one of the main sources of sodium in the diet and can carry dietary fiber. Variations in the addition of salt and fiber in the formulation of bread may require changes in amount of yeast. The nutritional information or label may influence its acceptability.

The objectives of this study have been: develop healthful optimized bread and evaluate its sensory acceptability blind and with label.

**Methods:** The optimized bread was successfully made: 35% less of NaCl added, 50% more of yeast and 75% more of fiber than white bread (in a previous study 15 prototypes had been evaluated using response surface methodology).

112 students and educational and non-educational staff of Facultad de Medicina, Odontología, Farmacia y Bioquímica, and Veterinaria of Universidad Buenos Aires tested it in two stages: blind and with label. Consumers evaluated appearance, smell, texture (crumb's elasticity), taste; and overall acceptance according to a 10-point scale (I=dislike extremely and 10=like extremely). An interval of 7 days was allowed between stages. Participants also answered: willingness to consume and day time in which they would consume it.

**Results:** The optimized bread was successfully developed. Participants were mainly students (92.8%), 82% female and median age 22 years (RI: 6).

The acceptability average values for attributes and overall acceptance were very good (all above 7). No differences were observed among the age groups. The average scores of willingness to consume were also very good (7 for blind stage, 7.3 with label), no differences between stages. Breakfast (29.7%), lunch (23.3%) and in-between meals (19%) were the most preferable moment to consume it. Cheese was most chosen for being consumed with the bread in-between meals.

**Conclusions:** It was possible to develop an optimized bakery that obtained good values of acceptability in the evaluation stages. This bread would appear to be an adequate strategy to increase the amount of dietary choices to prevent cardiovascular diseases.

**Keywords:** acceptability test – sodium reduction – dietary fiber – bread – optimization
CONTRIBUTION OF TRANS FATTY ACIDS BY
FOODS MOST PREFERABLY CONSUMED BY A
GROUP OF STUDENTS

(1)Mereles Ceuppens, Laura Graciela; (2)Coronel Mendez, Eva Eugenia; (2)Wiszovaty, Lourdes; (2)Caballero, Silvia; (2)Piris, Patricia; (2)Michajluk, Javier Boris; (3)Araujo Pino, Silvia Stella; (3)Gonzalez Cespedes, Laura Elizabeth; (3)Aguilar Rabito, Ana Carolina; (3)Morínigo Martínez, Macarena.

(1)PhD. Biochemistry. Departamento de Bioquímica de Alimentos.
Dirección de investigaciones. Facultad de Ciencias Químicas.
Universidad Nacional de Asunción. Paraguay; (2)Departamento
de Bioquímica de Alimentos. Dirección de investigaciones.
Facultad de Ciencias Químicas. Universidad
Asunción. Paraguay; (3)Departamento de Nutrición. Dirección
de investigaciones. Facultad de Ciencias Químicas. Universidad
Nacional de Asunción. Paraguay.

**Background and objectives:** The selection of food for col-
lege students is determined by socio-cultural characteristics, eco-
nomic availability and feasibility of access to them, which in gen-
eral have high fat contents, and all would indicate that also trans
fatty acids (TFA) from the partial hydrogenation of oils. The WHO
recommends that consumption not exceed 1% of VT of trans fat-
ty acids (TFA) as a strategy for prevention, reduction and control
of cardiovascular diseases. Therefore, the aim of the present work
was to identify the foods most preferably consumed by universi-
ty students of the Biochemistry career of the Faculty of Chemical
Sciences of the National University of Asunción and determine in
them the content of total AGT.

**Methods:** In 51 university students, we carried out a cross-sec-
tional study and applied a Food Consumption preference (FCP)
survey based on the LATINFOODS categories. For the quantifi-
cation of total TFAs, food samples were taken from canteens and
we used the Infrared Spectrometry (IR) method according to the
specifications of the official methods of AOAC (994.14. and 965.34).

**Results:** The average age of university students was 22.7 ± 2.8 years, 84% were female. Of the 60 foods in the FCP, we observed that the sandwich (with ingredients such as bread, butter, ham and cheese) was the food most frequently consumed in the week, followed by whole-grain crackers, baked goods and fried pies. The total TFA contents of industrial origin were: 2.64±0.24gTFA/100g in whole-grain crackers (brand A), 2.49±0.50gTFA/100g in whole crackers (brand B), 3.80±0.26 gAGT/100 g in the semi-defat-ted doughs, 3.20±0.14gAGT/100g in the frying pastry disks and 2.81±0.22gAGT/100g in sandwiches.

**Conclusions:** The AGT content varies by food group and serv-
ing size. All foods analyzed contain significant amounts of TFA
(≥0,2g/portion) and it was observed that TFAs of industrial origin
account for 15 to 39% of the fat in the foods analyzed. The results
obtained alert the need to reinforce the strategies to reduce the
content consumption of TFA in order to prevent cardiovascular
diseases.

**Keywords:** Trans fatty acids, contents, foods, preferential con-
sumption, students.

EFFECT OF GLYPHOSATE ON ACID LACTIC BAC-
TERIA

(1)Lourenço da Costa, Elizabete; (2)Silva, Caroline; (3)Antunes, Adriane; (4)Addad, João.

(1)Universidade Católica de Santos; (2)BSc. Nutritionist. Catholic University of Santos; (3)PhD. Nutritionist. School of Applied Sciences, University of Campinas; (4)PhD. Geologist. Technical Department. Tertium Brazil.

**Background and objectives:** Glyphosate is a broad spectrum systemic herbicide that is among the most widely used pesticides in the world. Due to its antimicrobial effect, its association with intestinal dysbiosis has been proposed, related to the etiology of intestinal inflammation, obesity, among other pathologies. The objective of this work was to evaluate the effect of glyphosate on five species of bacteria.

**Methods:** The microorganisms used were Bifidobacterium animalis, Lactobacillus acidophilus, L. rhamnosus, L. Casei and L. paracasei. Each single bacteria was reactivated in MRS broth, scattered on the surface of Petri dishes containing MRS agar, then sterile filter paper disks containing 15 µL of the herbicide (0.24%) were arranged on each plate. After incubation (36°C / 72 hours), the sensitivity was verified by the absence of growth around the disc. To obtain the growth curve, MRS broth tubes with increasing concentrations of the herbicide (0 to 0.03%) were incubated in a the same conditions previously described, with agitation, and the measurement of optical density (OD) in spectrophotometer (600nm) was carried out on each one hour, by 8 hours and after 24 hours.

**Results:** All the microorganisms tested showed sensitivity to the herbicide, both in the broth culture and in the agar diffusion assay. When bacteria were cultured without inhibition they showed a shift from the latency phase to the exponential phase after 4 hours of incubation, whereas in the presence of the herbicide containing glyphosate (0.03%, 0.0015% and 0.0075%), even after 24 hours of incubation there was no change in OD of broth cultures.

**Conclusions:** The present work confirms the susceptibility of the five strains of probiotics to the glyphosate herbicide even when the contact with low concentrations were used.

**Keywords:** acid lactic bacteria, glyphosate, herbicide, dysbi-
osis.
# THE IMPACT OF SOLAR MARKET GARDENS ON CHILD GROWTH, IRON AND VITAMIN A STATUS IN THE KALALÉ DISTRICT OF NORTHERN BENIN

(1)Taren, Douglas; (2)Alaofe, Halimatou; (3)Burney, Jennifer; (4)Naylor, Rosemond.

(1)PhD. Professor. Health Promotion Science. University of Arizona. USA; (2)PhD. Professor. Health Promotion Science. University of Arizona. USA.; (3)PhD. Professor. School of Global Policy & Strategy. University of California - San Diego. USA;

(4)PhD. Professor. Earth Energy and Environmental Sciences. Stanford University. USA.

**Background and objectives:** Developing a sustainable agricultural system to improve the nutritional status of children is a challenge in Sub-Saharan Africa where irrigation is labor intensive and crops are rainfed. The objective of this study was to evaluate how Solar Market Gardens (SMGs), a labor-saving solar power drip irrigation system at the village level, impacts the nutritional status of children.

**Methods:** The study was conducted in 8 intervention villages and 8 control villages using traditional hand-irrigation. Baseline and 1-year follow-up data were obtained for the same child (12-59 months of age) per household from 134 randomly-sampled control village households (C-H), 126 control village women group households (C-WG), 229 randomly sampled intervention village households using traditional irrigation (I-H) and 185 intervention village women's group households with SGMs (I-WG). Anthropometric measures and blood samples were collected to determine the impact of the SMGs on child growth, iron and vitamin A status by determining the impact estimate (IE) using difference-in-difference analysis.

**Results:** At baseline, children were 22.2 +/- 12.4 months of age, 23% were underweight, 36% were classified as stunted and 12.1% with wasting. Low hemoglobin concentrations (< 11g/L) were present in 80%, and 27.2% had a serum retinol < 0.70 mol/L. For all children, the I-WG households had a significant increase (p<0.05) in hemoglobin (IE 0.32) and weight-for-age z-scores (WAZ, IE 0.31) compared with all households, and a slight increase (p<0.10) for serum retinol (using RBP) compared with I-H. For children < 36 months of age at baseline, children from I-WG had a significant increase (p<0.01) for their WAZ (IE 0.44) and their height-for-age z-score (HAZ, IE 0.47) compared with all groups, and a slight increase (p<0.10) in their weight-for-height z-scores (WHZ, IE 0.41) compared with all groups and for serum retinol compared with I-H (IE 0.10).

**Conclusions:** SMGs appeared to have significantly supported the nutritional status of children in year 1 of the intervention. However, more time may be needed to obtain the full impact of SMGs. Measures of increased crop production, food security, income, and women's empowerment are potential mechanisms for the improved nutritional status.

**Keywords:** irrigation, horticulture, children, growth, micro-nutrients

# TOXICITY OF LEACHATE FROM PLASTIC PACKAGING IN TWO BIOLOGICAL MODELS

(1)dos Santos Gonçalves, Thaís; (2)Lourenço da Costa, Elizabete;

(3)Eduardo Addad, João.

(1)BSc. Nutritionist. Institute of Scientific and Technological Analysis. Catholic University of Santos. Brazil.; (2)PhD. Nutritionist. Institute of Scientific and Technological Analysis. Catholic University of Santos. Brazil.; (3)PhD. Geologist. Technical Department. Tertium. Brazil.

**Background and objectives:** Plastic occupies first place in the market of packaging for the food sector, guaranteeing better presentation to foodstuffs. However, to improve their aesthetic or technological features, metallization or addition of pigments to plastic packaging is employed, many of which may contain heavy metals as contaminants. The objective of this work was to evaluate the toxicity of plastic packaging leachates using the germination of lettuce seeds (Lactuca sativa) and Caenorhabditis elegans, a nematode widely used to evaluate the anthropogenic action.

**Methods:** Low density polyethylene packages, laminated or not pigmented, were chopped and kept in contact with solutions for leaching at different pH and time conditions. After filtration, an aliquot of the solutions obtained in each condition was used for analysis of metals (Cd, Cu, Pb and Cr) by atomic absorption spectrophotometry. Another aliquot was reserved for the biological assays, which included the germination of lettuce seed, which were placed in Petri dishes, on filter paper moistened with the solutions. After the incubation, the effect on the germination index was evaluated by measurement with a pachymeter. The conditions those caused, the greater interference on the seeds germination, were applied to observe the fecundity of the nematode C. elegans, put in contact from the embryonic stage.

**Results:** It was observed that the seeds placed in contact with the leachate obtained from laminated plastic packing presented alterations in their length, and reduction in egg production in C. elegans. The migration of cadmium to the aqueous medium was higher in the neutral leachate. But the interference in the metabolism of the studied organisms showed the greatest impact after the contact with the experimental condition obtained an acidified leachate from laminated plastic packaging.

**Conclusions:** The lack of correlation observed on better condition to metals migration and the one that showed the higher impact on organisms, points that probably others compounds from plastic polymer matrix has interfered on biological models, strengthening the perception about the limitation of chemical measurements. This finding is a worrying indicator, since a large number of foods in this type of packaging present a pH about 4.5, remaining stored for several months.

**Keywords:** Caenorhabditis elegans, Lactuca sativa, plastic packaging, heavy metal.
## ACCUMULATION PROCESSES OF NUTRITIONAL-
LY BENEFICIAL MINERALS IN A BROWN ALGAE,
HIJIKI (SARGASSUM FUSIFORME), DURING
GROWTH

(1)Katayama, Masayuki; (2)Sugawa-Katayama, Yohko; (3)Murakami, Kaori.

(1)PhD. Professor. Department of Health and Nutrition. Osaka Aoyama University. Japan.; (2)PhD. Professor. Department of Health and Nutrition. Osaka Aoyama University. Japan;

(3)PhD. Associate Professor. Department of Food Sciences and Biotechnology. Hiroshima Institute of Technology. Japan.

**Background and objectives:** The brown algae Hijiki, Sargassum fusiforme, is an important foodstuff for Japanese, and serves as a rich supply of minerals and dietary fiber. Warming of the earth elevates the ocean-water temperature year by year, and exerts ecological influences. Hijiki also seems to change their regular growing processes, becoming chlorotic earlier than before. To know the physiological changes during their growth, we studied the accumulating processes of several nutritionally beneficial minerals in Hijiki plants during their growth from the primary leaf stage to their chlorotic state. Although the accumulation of arsenic (As) occurred in plants during their growth, arsenic (As) could be mostly removed during the pre-cooking process of the commercial dried Hijiki products.

**Methods:** Hijiki plants were harvested at the seashore of Kusahimoto, Wakayama Prefecture, Japan. The fresh samples of Hijiki plants were lyophilized and decomposed with conc perchloric acid - conc nitric acid, or with conc sulfuric acid - conc nitric acid. The ashed samples were dissolved in 1N hydrochloric acid, and the concentrations of arsenic (As), calcium (Ca), iron (Fe), magnesium (Mg), manganese (Mn), potassium (K), and zinc (Zn) in the plant tissues were determined by atomic absorption spectrophotometry.

**Results:** The concentration of calcium (Ca) per tissue weight increased gradually coming to a rather constant value before the appearance of genital organs on the plants, although the concentration of arsenic (As) continued to increase. Until that time, the concentrations of zinc (Zn) and manganese (Mn) continued to increase further. The concentrations of iron (Fe) and magnesium (Mg) increased gradually, expressing a strong correlation between them, but the concentration of magnesium (Mg) was not uniform in the chlorotic plants.

**Conclusions:** From the viewpoint of nutritional value, matured plants of Hijiki had better be harvested, but to keep good quality of the products, fine days under the sunshine should be selected, even before complete maturation.

**Keywords:** Hijiki. Sargassum fusiforme. mineral accumulation. growing process.

## NUTRITION SENSITIVE AGRICULTURE FOR IMPROVING FOOD SECURITY: A CASE FROM AN-F4W

(1)Uddin, MD AMIN; (2)Asif, Chowdhury Abdullah Al; (3)Waid, Jillian;

(4)Stormer, Ame; (5)Mundy, Gary; (6)Sultana, Nigar; (7)Talukder, Aminuzzaman.

(1)Md Amin Uddin. (B.Sc. Ag. (Hons.), MBA). Director (Food Security & Livelihood). Chowdhury Abdullah Al Asif. MDS. Research and Evaluation Manager. Jillian L. Waid. Director Research Learning and Evaluation. Ame Stormer. PhD. Regional Director for Progra; (2)MDS. Research and Evaluation Manager.

Helen Keller International. Dhaka. Bangladesh.; (3)MSW. Director Research Learning and Evaluation. Helen Keller International.

Dhaka. Bangladesh.; (4)PhD. Regional Director for Programs. Regional Office. Helen Keller International. Phnom Penh.

Cambodia.; (5)MA. Regional Monitoring and Evaluation Advisor.

Regional Office. Phnom Penh. Cambodia; (6)MSc. Nutrition Specialist. Helen Kellen International. Dhaka. Bangladesh.;

(7)MPH. Country Director. Helen Keller International. Dhaka. Bangladesh.

**Background and objectives:** Since 2014, the ANF4W project in Bangladesh has been training small-scale farming households in agronomic bio-fortification of rice with zinc, and providing female farmers with training in homestead food production, nutrition education, gender empowerment and nutrition sensitive agriculture. This study was conducted to evaluate the factors influencing food security among participants.

**Methods:** A panel survey was conducted among 332 female farmers, consisting of 156 households that had been participating in the ANF4W program for 2 years, and 176 households that had been participating for one year. We used the Household Food Insecurity Assessment Scale (HFIAS) to assess food security, di-chotomizing values into ‘food secure’ and ‘food insecure’. We ran a logistic regression model to test associations between food security and household size, women’s decision- making ability in the household, self-reported years of experience in home gardening, length of involvement in the project, size of cultivated land, and dietary adequacy of female farmers.

**Results:** We found that years of experience in home gardening (OR -0.006, p<.05) and women reporting that they have decision making power about major household purchases (OR -1.068, p<.05) were positively and significantly associated with households being food secure. No other variables were found to be significantly associated with food security.

**Conclusions:** More experienced home gardeners have greater resilience against food insecurity, but this experience was not a result of project participation. It is possible that the length of time for both groups was not sufficient to show a difference or an impact; further, longer term investigation is needed. Households where women have a greater role in major household purchases are more likely to be food secure, suggesting that there are benefits to agriculture and nutrition programs focusing on empowering women beyond the sphere of agricultural production.
**Keywords:** Food security, women's decision making, home gardening.

**Further collaborators:**

Deutsche Gesellschaft fuer internationale Zusammenarbeit (GIZ) GmbH. Germany.

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## QUALITY CHARACTERISTICS, CONSUMPTION PATTERNS AND NUTRIENT PROFILE OF YELLOW FLESH CASSAVA VARIETIES AND THEIR PRODUCTS

(1)Akwaa Harrison, Obed; (2)Duah, Elizabeth; (3)Baah, Rose; (4)Parks, Elizabeth); (5)Saalia, Firibu; (6)Steiner-Asiedu, Matilda.

(1)Mphil Nutrition (Final year). Department of Nutrition and Food Science. University of Ghana. Accra-Legon; (2)Mphil Nutrition. Department of Nutrition and Food Science. University of Ghana, Legon.; (3)Bsc. Nutrition. Department of Nutrition and Food Science. University of Ghana, Legon.; (4)PhD. Harvestplus cassava Breeder. International Institute of Tropical Agriculture. PMB 5320. Ibadan 200001. Oyo State. Nigeria.; (5)(PhD). Associate Professor in Food Science. Head of Department of Food Engineering and Processing Sciences. Senior Lecturer. Department of Nutrition and Food Science. University of Ghana. Accra. Ghana.; (6)Associate Professor in Nutrition. President of Ghana Nutrition Association. Dean of School of Biological Sciences. Senior Lecturer. Department of Nutrition and Food Science. University of Ghana. Accra. Ghana.

**Background and objectives:** Cassava is a popular root crop in the world which contributes to the staple of most populations in the subtropics and contributes significantly to food and nutrition security in deprived populations since both roots and leaves are eaten. Additionally, cassava leaves are excellent source of proteins, vitamins (B1, B2, C) and essential minerals (iron and zinc). Yellow-fleshed varieties with high carotenoids content have been developed with the aim of combating hidden hunger. The study aimed at determining the carotenoid content of fresh cassava roots, processed products (gari and kokonte) from the roots, including the iron and zinc contents of the cassava leaves.

**Methods:** Fifteen(15) varieties of fresh yellow flesh cassava (YFC) roots and leaves were obtained from the Crop Research Institute. 5 of samples 301, 303, 305, 307, and 315 were chosen by the processors' analysis. Total carotenoids was determined using the ICHECKTM carotene device. The TCC of gari and kokonte were determined as described by BioAnalyt (2014). Iron and zinc contents were determined using AOAC(2005) methods. A consumer acceptability was done using 5 a point hedonic scale for gari samples in terms of their overall acceptability, appearance, aroma, flavour, and mouthfeel

**Results:** The processor's TCC of the fresh YFC samples ranged between 10.12 mg/kg to 4.68 mg/kg. Gari from sample 301 had the highest TCC of 7.67 mg/kg whereas sample 305 had the least value

of 4.82 mg/kg however that in kokonte was too small to be detected. Boiled cassava had highest TCC of 2.16 mg/kg and least of 1.24 mg/kg for samples 315 and 305 respectively. The iron content of fresh cassava leaves was between 3.89 ± 0.01 µg/g and 1.51 ± 0.01 µg/g for samples 310 and 308 respectively. The zinc content of cassava leaves ranged from 0.42 ± 0.04 µg/g for sample 310 to 0.05 ± 0.01 µg/g for sample 308. More than half of the respondents (51.2 %) were willing to accept the yellow flesh cassava as well as use it.

**Conclusions:** Thus YFC has potential in enhancing Food and Nutrition security in Ghana and beyond by curbing hidden hunger as judged by the preliminary studies.

**Keywords:** Cassava, bio-fortification, food and nutrition security, carotenoids

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## NUTRITIONAL VALUE OF SNACKS COMMERCIALIZED IN PRIVATE UNIVERSITY IN SÃO PAULO CITY

(1)Poltronieri, Fabiana; (2)Pádua, Amanda Batista Vilas Boas;

(2)Carnelossi, Ana Cecília; (2)Olhera, Camila Carolina Bernardes;

(2)Massignani, Giuliana Cravero; (2)Barroso, Helainne Kristina Benedito.

(1)PhD. Nutritionist. Faculdade das Américas. FAM.; (2)Nutritionist. São Paulo. SP. Brazil.

**Background and objectives:** The brazilian population's eating habits indicates that the consumption of processed foods and ready meals increased, and traditional foods such as rice and beans reduced their participation in the daily calories of brazilians' intake. High consumption of fast foods, including snack foods, has worried public health officials about the prevalence increase of noncommunicable chronic diseases. The objective of this work was to determine the chemical composition of samples of snack foods marketed in private university (São Paulo-SP).

**Methods:** Approximately 500g of 4 different kinds of snacks were collected: 2 fried (rissoles, “coxinha”), and 2 baked snacks (esfiha, croissant). The snacks weighted 150g each. The percentages of moisture, proteins, lipids, ashes and carbohydrates of the samples were determined by chemical analysis. The total dietary fiber was estimated based on the brazilian table of food composition.

**Results:** The moisture of the analyzed samples ranged from 45.98 ± 0.09 to 73.46 ± 0.12. The rissoles had the highest ashes concentration (1.95 ± 0.03) and the croissant the lowest (0.66 ± 0.00). The sample “coxinha” had the highest lipid concentration (6.38 ± 0.11), and croissant the lowest (3.13 ± 0.03). However, the croissant showed the lowest protein concentration (3.70 ± 0.04), and “coxinha”, the highest (8.58 ± 0.03). The total dietary fiber content did not reach 1% in all samples analysed. The concentration of carbohydrates, obtained by difference, was approximately 18% in all samples, except for the esfiha that was almost 38%.

**Conclusions:** The results revealed that the snacks had high lipid, and low dietary fiber concentration. Therefore, the daily consumption of these foods is a nutritionally inadequate option.

**Keywords:** food composition, snack foods
# BIOACCESSIBILITY AND TOTAL CONTENT OF TRACE ELEMENTS IN DIFFERENT VARIETIES OF COOKED RICE

(1)Ortiz, Maria Laura; (2)Cámara Martos, Fernando; (3)Brem, Juan José; (3)Trulls, Horacio.

(1)MSc. Biochemist. Department of Basic Sciences. Universidad Nacional del Nordeste. Argentina; (2)PhD. Department of Bromatology and Food Technology. Universidad de Córdoba. Spain.; (3)PhD. Veterinarian. Department of Basic Sciences. Facultad de Ciencias Veterinarias. Universidad Nacional del Nordeste. Corrientes. Argentina.

**Background and objectives:** Rice (Oryza sativa) is a crop of great, economic and social importance worldwide. At present, the study of trace elements in foods, both beneficial to health and potentially toxic, has been prioritized, and rice grain is being one of the cereals characterized by a selective accumulation of these elements. The total concentration of the different trace elements in food to be consumed, allows estimate the dose of exposure, while the soluble fraction present in the intestinal lumen after the digestive process, shows its bioaccessibility, so this is a more precise nutritional evaluation of the food. The objective of the present work was to determine the total and bioaccessible concentration of iron (Fe), copper (Cu), manganese (Mn) and zinc (Zn) in samples of different varieties of cooked, lyophilized and ground rice.

**Methods:** The bioaccessible fraction was determined by an in vitro solubility method, the total and solubilized minerals, after ashing, were determined by Flame Atomic Absorption Spectrophotometry.

**Results:** The results were ranges of total values (µg / g) of Cu: 1.45-5.59; Mn: 2.45-13.61; Zn: 8.82-12.93 and Fe: 6.50-15.28, while the percentages of solubilized and bioavailable minerals were Cu: 24.01-79.30%; Mn: 5.30 - 27.31%; Zn: 6.70-22.53% and Fe: 24.80-92.60%.

**Conclusions:** It is concluded that, both, the total content of minerals as the soluble fraction to be used by the organism, are influenced by the different physical and chemical characteristics of the studied varieties, so the brown rice, with high fiber content, has showed the lowest bioaccessibility percentage values of these studied trace elements.

**Keywords:** Bioaccessibility, rice, varieties, minerals, soluble fraction.

**Further collaborators:**

Zach, Astrid. Brem, José Carlos

# THE IMPACT OF SOLAR MARKET GARDENS ON DIETARY DIVERSITY, WOMEN'S NUTRITIONAL STATUS AND COMPONENTS OF WOMEN'S EMPOWERMENT IN THE KALALÉ DISTRICT OF NORTHERN BENIN

(1)Alaofe, Halimatou; (2)Douglas, Taren; (3)Burney, Jennifer; (4)Naylor, Rosemond.

(1)PhD. Nutritionist. Health Promotion Sciences. University of Arizona. US; (2)PhD. Nutritionist. Health Promotion Science. University of Arizona. US; (3)PhD. Environmental Scientist. School of Global Policy & Strategy, University of California - San Diego. US; (4)PhD. Economist. Earth Energy and Environmental Sciences. Stanford University. US.

**Background and objectives:** Rainfed agriculture poses growing production risks with increased climate variability and change in Sub-Saharan Africa (SSA), largely due to low use of irrigation and fertilizer. However, to date, few studies have analyzed the impact of irrigation interventions on nutrition, health, and women's empowerment, despite the large potential of irrigation to increase agricultural production. The objective of this study was to evaluate the impact of Solar Market Gardens (SMGs) on dietary diversity, women's nutritional status and components of women's empowerment in the Kalalé district of Northern Benin.

**Methods:** The study was conducted in 8 intervention villages and 8 control villages using traditional methods. Baseline and 1-year follow-up data were obtained for childbearing age women (15-45 years old) from 185 SMGs women's group (WG) households and randomly selected 229 non women's group (NWG) households, 126 control women's group (CWG) and 234 control non women's group (CNWG) households. Collected nutrition-related indicators included household (HDDS) and women (WDDS) dietary diversity score, body mass index (BMI), prevalence of thinness (BMI<18.5 kg/m2), anemia, iron (ID) and vitamin A (VAD) deficiencies, while women's empowerment measures included decision making, physical mobility, male household involvement, self-confidence, economic independence and group membership components. The Impact estimate (IE) of the SMGs was determined using difference-in-difference between WG and control households (NWG, CWG, and CNWG).

**Results:** The SMGs women's group households were associated with a significant increase in HDDS (IE 0.36), WDDS (IE 0.47), and a significant reduction in anemia prevalence (IE -0.77), whereas there was no significant impact on BMI, thinness, ID and VAD (p>0.05). SMGs women had also greater self-confidence (IE 0.58), decision making (IE 0.74), and group membership (IE 0.66), but no impact was observed with physical mobility, male household involvement, and economic independence components of women's empowerment.

**Conclusions:** Solar Market Gardens have the potential to improve women's nutritional status and components of women's empowerment in the Kalalé district of Northern Benin. However, the lack of impact on BMI, iron and vitamin A deficiencies, and economic independence components of women's empowerment
points out the urgent need for behavior changes communication interventions to improve women nutrition status.

**Keywords:** irrigation; dietary diversity; women's nutrition status; women's empowerment; Benin

(1)Andrews-Trevino, Johanna Y; (2)Webb, Patrick; (3)Shrestha, Robin;

(4)Shively, Gerald; (5)Rogers, Beatrice; (6)Davis, Dale; (7)Pokharel, Ashish; (8)Paudel, Krishna; (9)Baral, Kedar Prasad; (10)Ghosh, Shibani.

(1)MSc. Nutritionist. Gerald J. and Dorothy R. Friedman School of Nutrition Science and Policy. Tufts University. USA; (2)PhD. Director of the Innovation Lab for Nutrition. Gerald J. and Dorothy R. Friedman School of Nutrition Science and Policy. Tufts University. USA; (3)MD/MSc. Project Manager - Asia-Africa for the Innovation Lab for Nutrition. Gerald J. and Dorothy R. Friedman School of Nutrition Science and Policy. Tufts University. USA; (4)PhD. Associate Department Head / Professor. Department of Agricultural Economics and Purdue Policy Research Institute. Purdue University. USA; (5)PhD. Professor and Program Director - Food Policy and Applied Nutrition. Gerald J. and Dorothy R. Friedman School of Nutrition Science and Policy. Tufts University. USA.; (6)MSc. Country Director. Helen Keller International. Nepal.; (7)MSc. Research Manager. Helen Keller International/Feed the Future Innovation Lab for Nutrition-Nepal. Nepal.; (8)MD. Chief Consultant Pediatrician/Assistant Professor. Kanti Hospital. Nepal.; (9)MD. Rector. Patan Academy of Health Sciences. Nepal.; (10)PhD. Associate Director of the Innovation Lab for Nutrition. Gerald J. and Dorothy R. Friedman School of Nutrition Science and Policy. Tufts University. USA.

**Background and objectives:** Aflatoxin exposure (AFB1) has been linked to poor growth patterns in infants and young children; however, little is known of the relationship of aflatoxin exposure in pregnancy and low birth weight (LBW). This study estimated the prevalence of LBW (< 2.5 kilograms) and assessed the association of aflatoxin exposure in pregnancy and LBW prevalence.

**Methods:** Data from newborn infants enrolled in an ongoing USAID-supported birth cohort study in Banke, Nepal, was used for this analysis. Only infants measured within 72 hours of birth were included in this analysis (n=1484, 96% of total recruited). Data on AFB1, socio-demographics, anthropometry and health were collected during the pre-natal visit. Binary logistic regression was used to identify potential risk factors associated with LBW. Statistical analyses were conducted with Stata® SE version 14.

**Results:** Approximately 20% of the infants were born with a LBW. The average AFB1-lysine adduct levels in maternal serum were 3.4 (±8.4) pg/mg for women with LBW infants and 3.1 (±8.1) pg/mg for women with normal birth weight infants. Significant bivariate associations were found between: LBW and maternal aflatoxin levels, maternal schooling, dietary diversity score, wealth

quintile, short stature (<145 centimeters), hemoglobin, mid-upper arm circumference (MUAC) and female child. After adjusting for socio-demographic variables and potential confounding factors, findings showed the odds of a LBW infant increased with increases of AFB1 albumin adducts (OR=1.14; 95% CI: 1.01-1.28 p=0.031). Moreover, the odds of having a LBW infant decreased with each additional year of education (OR=0.95; 95% CI: 0.91-0.98, p=0.001), each unit increase in dietary diversity score (OR=0.84; 95% CI: 0.76-0.93, p=0.001) and each centimeter increase in MUAC (OR=0.89; 95% CI: 0.83-0.94, p=0.000). Short maternal stature (OR=2.05; 95% CI: 1.45-2.89, p=0.000) and female gender (OR=1.42; 95% CI: 1.08-1.85, p=0.011) increased the odds of LBW.

**Conclusions:** The results indicate a high prevalence of LBW among the study population and confirm that aflatoxins may increase the risk of adverse birth outcomes such as LBW. Findings suggest that in addition to increased education and improved maternal anthropometry, interventions to reduce LBW should encourage improvements to safe and balanced diets before and during pregnancy.

**Keywords:** aflatoxin, low birth weight, Nepal

(1)Kader, Abdul; (2)Abdullah Al Asif, Chowdhury; (3)Hasan, Md. Mehedi; (4)Ali, Masum; (5)Rahman, Arafat; (6)Waid, Jillian.

(1)MPH, Deputy Director - Research, Learning, and Evaluation, Helen Keller International, Bangladesh Country Office; (2)MPH, Monitoring and Evaluation Manager, Helen Keller International, Bangladesh; (3)MPH, Sr. Program and Research Analyst, Helen Keller International, Bangladesh; (4)MA, Program and Research Analyst, Helen Keller International, Bangladesh; (5)MA, Sr. Program and Research Analyst, Helen Keller International, Bangladesh; (6)MSW, Director - Research, Learning, and Evaluation, Helen Keller International, Bangladesh.

**Background and objectives:** Helen Keller International Bangladesh implemented the Making Markets Work for Women (M²W²) project in two sub-districts of the Chittagong Hill Tracts (CHT). The goal of the project was to eliminate extreme poverty through introducing sustainable agricultural and improved animal husbandry and building marketing skills. We seek to measure the impact of this project on income, food security, and diets, as well as elements of behavior change along the pathway among the population in these areas as a whole.

**Methods:** We used propensity score matching methods to identify 24 control villages that were the most similar to 24 of the 72 villages M2W2 worked in, based on distance from the road, ethnic makeup, population size, literacy rates, quality of housing, and quality of sanitation facilities. From each of these 48 villages we surveyed 30 households three times in order to estimate the
impact of the project. The first survey was after two years of the intervention (October 2015), followed by two rounds of data collection in the year following the scale down of the intervention. This analysis only utilizes the first survey round.

**Results:** In treatment villages, an average of 36% of surveyed households were directly involved in the program (range 6 to 94%). In treatment communities, a greater proportion of households were involved in agriculture (90% vrs. 76%, p<0.02), had livestock (82% vrs. 71.9, p<0.05), had savings (58% vrs. 38%, p<0.01), had daily percapita expenditure above the poverty line (52% vrs. 42%, p<0.01), and had more months of adequate food provisioning (11.4 vrs. 11.0, p<0.02). There was no significant impact on women's dietary diversity (4.2 vrs. 4.1, p<0.25) and on mean percapita expenditure (79 vrs. 74 taka, p<0.40).

**Conclusions:** There is evidence of some project impact both along the causal pathway and in increasing food security, limited impacts in improving income, and no significant impact in improving diet. Future work will use difference-in-difference analysis to examine differences in the trajectory of change between treatment and control communities, and participant and non-participant households in treatment communities over a longer time period.

**Keywords:** food security, dietary diversity, evaluation, impact pathways

**Further collaborators:**

This abstract was produced by Helen Keller International through support provided by PATH, under a grant from UK aid from the UK Government; however, the views expressed do not necessarily reflect the UK government's official policies.

**Methods:** The ecto-domains of human, murine, and bovine Btn1a1 were expressed as fusion proteins with immunoglobulin Fc fragment, respectively. The gene coding the ecto-domain of Btn1a1 was subcloned into the expression vector pFUSE-hIgG1e3-Fc and Expi293 mammalian cells were transfected with the expression vectors. The Btn1a1 fusion proteins secreted into the culture supernatant were purified by a protein G column. HT29-MTX-E12 cells were cultured for 21 days to differentiate into mucin-producing cells. RNA was extracted from HT29-MTX-E12 cells to quantitate the gene expression level of mucins by real-time PCR after Btn fusion proteins were added. Lectin assay was used to quantitate mucins secreted in the culture supernatant.

**Results:** Expression level of the genes muc5ac and muc17 significantly increased 0.5 to 2 hours immediately after the addition of a fusion protein regardless of species-specificity of Btn1a1. The level of secreted mucins was markedly promoted by the addition of any kind of fusion protein in the dose and time-dependent manner.

**Conclusions:** Considering that mucins can exert as a barrier against bacteria and viruses infected, we suggest that mucosal homeostasis of the infants may be physiologically controlled by Btn1a1 molecules in milk fat globules.

**Keywords:** butyrophilin; milk fat globule membrane; mucin; intestinal epithelial cell

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BUTYROPHILIN ECTO-DOMAIN OF MILK FAT GLOBULE MEMBRANE PROMOTES MUCIN PRODUCTION FROM THE INTESTINAL EPITHELIAL CELLS

(1)Ogawa, Takenobu; (2)Namgung, Hyejin; (3)Kuwana, Yuki; (3)Furue, Ayami; (1)Masuda, Tetsuya; (4)Shirai, Nobuaki; (1)Tani, Fumito.

(1)PhD. Graduate School of Agriculture. Kyoto University. Japan.;

(2)BSc. Graduate School of Agriculture. Kyoto University. Japan.;

(3)MSc. Graduate School of Agriculture. Kyoto University. Japan.;

(4)PhD. Graduate School of Agriculture. Kyoto University. Japan; Industrial Research Center of Shiga Prefecture. Japan.

**Background and objectives:** Butyrophilin (Btn) was first discovered as a transmembrane protein in milk fat globule membrane. This archetypal Btn (Btn1a1) is also known to modulate the production and the secretion of milk fat globules in the epithelial cells of mammary glands. Recently the primary sequence of the ecto-domain of Btn1a1 was found to have a homology to that of the co-stimulatory B7 molecule, so that many homologs of Btn1a1 have been identified so far. Btn and Btn-like proteins are expressed on the surface of intestinal epithelial cells as well as T cells. The aim of this study is to examine how Btn1a1 functions on the epithelial cells in the gastrointestinal tract.

MAINSTREAMING NUTRITION INTO REGIONAL AGRICULTURE INVESTMENT POLICY AND STRATEGY OF THE ECONOMIC COMMUNITY OF WEST AFRICAN STATES (ECOWAS) 2016-2025

(1)Giyose, Boitshepo; (2)Seki, Richemont; (2)Sablah, Mawuli; (3)Ag Bendech, Mohamed; (4)Dufour, Charlotte.

(1)Senior Nutrition Officer. Policy and Porgammes. UNFAO. Italy;

(2)Nutrition Officer. UNFAO. Ghana; (3)Senior Nutrition Officer. UNFAO. Ghana; (4)Nutrition Officer. UNFAO. Italy.

**Background and objectives:** With persistent high levels of undernourishment and malnutrition in West Africa, it is necessary to invest in every sector that could contribute to reverse the situation. Agriculture remains a very important sector, which can significantly influence the nutrition status of West African population. It is important to ensure that any agricultural strategy or interventions are tailored to have nutrition objectives and impacts. West Africa's agriculture has shown some improvements in its performance, resulting in a drastic reduction of undernourishment from 24% in 1990-1992 to 10% in 2014-2016. ECOWAS and its member states have embarked on a multi-sectoral planning exercise that will lead to the formulation and implementation of a new and second phase of ECOWAP (2016-2025) that will better integrate nutrition aspects.

**Methods:** Strong advocacy at political and technical levels, as well as sharing of guidance materials and best practices were undertaken by relevant institutions, including, the Food and Ag-