# Innovative approaches: phyto-probiotics as a therapeutic support for managing obesity-linked psoriasis

Arthur Chervet, Rawan Nehme, Clémence Defois-Fraysse, Caroline Decombat, Bertrand Evrard, Edith Filaire, Jean-Yves Berthon, Assia Dreux-Zigha, Laetitia Delort, Florence Caldefie-Chezet

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Arthur Chervet, Rawan Nehme, Clémence Defois-Fraysse, Caroline Decombat, Bertrand Evrard, et al.. Innovative approaches: phyto-probiotics as a therapeutic support for managing obesity-linked psoriasis. Journée des écoles doctorales (JED), Jun 2024, Clermont-Ferrand, France. hal-04878925

HAL Id: hal-04878925

https://hal.science/hal-04878925v1

Submitted on 13 Jan 2025

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# Innovative Approaches: Phyto-Probiotics as a Therapeutic Support for Managing Obesity-Linked Psoriasis

A. Chervet¹, R. Nehme¹, C. Defois-Fraysse², C. Decombat¹, B. Evrard¹, E. Filaire¹, J.-Y. Berthon³, A. Dreux-Zigha², L. Delort¹, A. F. Caldefie-Chezet¹

¹INRAE, UNH, Unité de Nutrition Humaine, Université Clermont-Auvergne, Clermont-Ferrand, ²Greencell, Saint-Beauzire, France, ³Greentech SA, Saint-Beauzire, France

## Background

Obesity, which has become a global public health issue, is defined by an excessive accumulation of body fat. It is a chronic disease and a major risk factor for many other pathologies development such as psoriasis. Obesity epidemiology demonstrates that its prevalence has gradually increased over the last few decades. According to WHO, more than 890 million persons are estimated to be obese in 2022.

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Keratinocyte activation and clinical manifestations of psoriasis

### PP31 (Phyto-Probiotics 31 production)

<figure><img src="image_3.png" /></figure>

## Targets

Low-grade Inflammation

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Obesity

<figure><img src="image_6.png" /></figure>

Psoriasis

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## Methods & Results

### Leptin, adiponectin and HSL (hormone-sensitive lipase) expression on human mature adipospheroids

<figure><img src="image_8.png" /></figure>

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Decreased the gene expression of Leptin and enhanced HSL and Adiponectin in human mature adipospheroids

Leptin: pro-inflammatory adipokine

Adiponectin: anti-inflammatory adipokine

HSL: Enzyme that converts triglycerides in adipose tissue into fatty acids

### THP-1 polarisation into M0-like or M1-like macrophages

<figure><img src="image_10.png" /></figure>

<figure><img src="image_11.png" /></figure>

Reduced the gene expression of M1-like markers (TNF-α, IL-6, IL-8, IL-1β & CXCL10)

### Psoriatic keratinocytes

<figure><img src="image_12.png" /></figure>

<figure><img src="image_13.png" /></figure>

Lowered the secretion of IL-6 & IL-1β, not significant for IL-8 and S100A7

S100A7 (psoriasin): bacteriocin up-regulated in patients with psoriasis

### Co-culture mature obese adipospheroids / psoriatic keratinocytes

<figure><img src="image_14.png" /></figure>

In order to mimic the influence of the obese adipose microenvironment on psoriasis, mature adipospheroids (BMI>31) were co-cultured with psoriatic keratinocytes from biopsies. The adipospheroids were grown and differentiated for 10 days before being co-cultured with psoriatic keratinocytes. On the day of co-culture, the extract was applied for 24 hours.

<figure><img src="image_15.png" /></figure>

Decreased the gene expression of IL-6, IL-8, IL-1β, S100A7 in Psoriatic keratinocytes.

Downregulated IL-6, IL-1β in adipospheroids

<figure><img src="image_16.png" /></figure>

Decreased the secretion of IL-6, IL-8, IL-1β, S100A7

## Conclusion

PP31 extract may exhibit a triple effect by targeting obesity, inflammation, and psoriasis biomarkers, and may help in both local and systemic management of obesity-linked psoriasis.