# Podapolipoides chorthippus n. sp. (Acari: Prostigmata: Podapolipidae), an ectoparasite of Chorthippus sp.

## (Orthoptera: Acrididae) from southern Iran

Maryam Majidi, Hamidreza Hajiqanbar, Alireza Saboori

► To cite this version:

Maryam Majidi, Hamidreza Hajiqanbar, Alireza Saboori. Podapolipoides chorthippus n. sp. (Acari: Prostigmata: Podapolipidae), an ectoparasite of Chorthippus sp. (Orthoptera: Acrididae) from southern Iran. Acarologia, 2022, 62 (1), pp.120-129. 10.24349/38vh-n9rc. hal-03547679

HAL Id: hal-03547679

https://hal.science/hal-03547679

Submitted on 28 Jan 2022

HAL is a multi-disciplinary open access
archive for the deposit and dissemination of sci-
entific research documents, whether they are pub-
lished or not. The documents may come from
teaching and research institutions in France or
abroad, or from public or private research centers.

L'archive ouverte pluridisciplinaire HAL, est
destinée au dépôt et à la diffusion de documents
scientifiques de niveau recherche, publiés ou non,
émanant des établissements d'enseignement et de
recherche français ou étrangers, des laboratoires
publics ou privés.

<figure><img src="image_1.png" /></figure>

Distributed under a Creative Commons Attribution 4.0 International License
<figure><img src="image_2.png" /></figure>

Acarologia
Open Science in Acarology

<figure><img src="image_3.png" /></figure>

Acarologia

A quarterly journal of acarology, since 1959
Publishing on all aspects of the Acari

All information:
http://www1.montpellier.inra.fr/CBGP/acarologia/
acarologia-contact@supagro.fr

<figure><img src="image_4.png" /></figure>

OPEN ACCESS

Acarologia is proudly non-profit,
with no page charges and free open access

Please help us maintain this system by
encouraging your institutes to subscribe to the print version of the journal
and by sending us your high quality research on the Acari.

Subscriptions: Year 2022 (Volume 62): 450 €
http://www1.montpellier.inra.fr/CBGP/acarologia/subscribe.php

Previous volumes (2010-2020): 250 € / year (4 issues)
Acarologia, CBGP, CS 30016, 34988 MONTFERRIER-sur-LEZ Cedex, France
ISSN 0044-586X (print), ISSN 2107-7207 (electronic)

The digitalization of Acarologia papers prior to 2000 was supported by Agropolis Fondation under the reference ID 1500-024 through the « Investissements d'avenir » programme
(Labex Agro: ANR-10-LABX-0001-01)

<figure><img src="image_5.png" /></figure>

<figure><img src="image_6.png" /></figure>

Acarologia is under **free license** and distributed under the terms of the
Creative Commons-BY
# *Podapolipoides chorthippus* n. sp.
(Acari: Prostigmata: Podapolipidae),
an ectoparasite of *Chorthippus* sp.
(Orthoptera: Acrididae) from southern
Iran

Maryam Majidiª, Hamidreza Hajiqanbarª, Alireza Sabooriᵇ

## Original research

### ABSTRACT

The ectoparasitic mite *Podapolipoides chorthippus* Majidi and Hajiqanbar **n. sp.** (Acari: Prostigmata: Podapolipidae), is described and illustrated from Fars province, southern Iran. Colonies of mites were recovered from pronotum and near hindwing bases of acridid grasshoppers, *Chorthippus* sp. (Orthoptera: Acrididae: Gomphocerinae). This is the first parasitism record of the genus *Podapolipoides* on grasshoppers of the genus *Chorthippus*.

**Keywords** ectoparasite; mite; Heterostigmata; grasshoppers; Gomphocerinae

**Zoobank** http://zoobank.org/A4CEBBCB-0059-4259-A824-8FBCB4408D0D

This article is dedicated to the memory of Hamidreza Hajiqanbar who unfortunately passed away after submitting the manuscript, on 14 October 2021 in a tragic car crash.

## Introduction

Symbiotic relationships, including parasitism, with arthropods have been frequently evolved among different groups of mites. One of the most sophisticated ones is Prostigmata (Acari: Acariformes) which is comprised of species that occupy various habitats (Walter *et al.* 2009). Some species of this lineage are phoretic or parasitic on insects, mostly classified in Parasitengona and Heterostigmata cohorts, yet some other families like Cheyletidae and Dytiscacaridae have evolved parasitism (Walter *et al.* 2009; Mortazavi *et al.* 2018). Within Heterostigmata, the family Podapolipidae Ewing, 1922 with 32 genera and more than 290 described species, is one of the largest families in Prostigmata (Majidi *et al.* 2019). Members of this family are highly specialized obligate parasites of insects of five orders namely, Coleoptera, Orthoptera, Blattodea, Heteroptera and Hymenoptera (Husband & OConnor 2014; Seeman 2021).

Five genera of podapolipids parasitise orthopterans: *Locustacarus* Ewing, 1924 with four species (Rahmatzaei *et al.* 2021); *Podapolipus* Rovelli & Grassi, 1888 with 31 species (Lindquist & Sidorchuk 2019); *Podapolipoides* Regenfuss, 1968 with 31 species (Hajiqanbar & Joharchi 2011); *Orthapolipus* Husband & Li, 1993 with 3 species (Husband *et al.* 2005) and monobasic *Wetapolipus* Husband & Zhang, 2002 (Husband & Zhang 2002). The genus *Podapolipoides* is confined to grasshopper of the families Acrididae (mostly) and Pyrgomorphidae (Husband 1995). So far, *P. grassii* (Berlese, 1897) on *Locusta migratoria* L. and different acridids and *P. anacridii* Hajiqanbar & Joharchi, 2011 on *Anacridium* sp. have been reported from Iran (Ostovan & Saboori 1999; Hajiqanbar & Joharchi 2011). In this study, we described a new species of *Podapolipoides* recovered from the acridid grasshopper, *Chorthippus* sp., from southern Iran and compared it with morphologically closely related species.

<figure><img src="image_7.png" /></figure>

<footnote>ªDepartment of Entomology, Faculty of Agriculture, Tarbiat Modares University, 14115-336, Tehran, Iran.</footnote>

<footnote>ᵇDepartment of Plant Protection, Faculty of Agriculture, University of Tehran, Karaj, Iran.</footnote>
<figure><img src="image_8.png" /><figcaption>**Figure 1** A colony of *Podapolipoides chorthippus* n. sp. on pronotum of *Chorthippus* sp.</figcaption></figure>

**Materials and Methods**

Collected grasshoppers were preserved in vials with 96% ethanol. Subsequently, grasshoppers and alcohol sediments from the vials were examined for parasitic mites. Host grasshoppers were collected from Latifi region, located in Fars province, Southern Iran in 2017. Colonies of mites were separated from pronotum and near wing bases of acridid grasshoppers, *Chorthippus* sp. (Orthoptera: Acrididae: Gomphocerinae) (Figure 1). Mite specimens were cleared in Nesbitt's solution and mounted in Hoyer's medium. Morphology of the mites was studied using a compound microscope (Olympus BX51, Tokyo, Japan) equipped with phase contrast illumination. Photographs were taken with the aid of Samsung i70 digital camera. All measurements in the descriptions are given in micrometers for the holotype and five paratypes (in parentheses). The terminology follows Lindquist (1986). Details of geographical position have been recorded using GPS mobile device. Setae with no longer than the diameters of setal acetabulae are considered as microsetae and those with only acetabulae and no setal remnants are assigned as vestigial setae. The host insect was identified with the help of Dr. Mustafa Ünal (Abant İzzet Baysal Üniversitesi, Turkey).

**Results**

**Family Podapolipidae Ewing, 1922**

**Genus Podapolipoides Regenfuss, 1968**

Type-species: *Podapolipoides (Podapolipus) grassii* (Berlese, 1897), by original designation.
Podapolipoides chorthippus Majidi and Hajiqanbar n. sp.

Zoobank: 6EC39A40-9645-4C0F-8570-153EFE16AD3F

Diagnosis

Larval females with dorsal setae *d* (7–8) short, simple and pointed; *f* serrate; *h*₂ simple, pointed and slightly thickened; *v*₁ (2–4), *v*₂ (5–10); 1*a* m, 2*a* m, 3*b* m (m-4). Males with dorsal setae *sc*₂ 86 and *c*₂ 118 long, *sc*₂ extending beyond bases of setae *c*₂.

Type-host

*Chorthippus* sp. (Orthoptera: Acrididae: Gomphocerinae). Attachment site on host: On pronotum and near wing bases (Figure 1).

Type-material

Holotype female (MM20170303) and paratypes (9 adult females, 1 male, 22 larval females) found in Fars Province, Southern Iran, 27°41′N, 54°24′ E and altitude 799 m, 3 III 2017; collected by Maryam Majidi; deposited in the Acarological Collection, Department of Entomology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran. One adult female and one larval female are deposited in Zoological Institute, University of Hamburg, Hamburg, Germany.

Description

Adult female — (Figure 2)

*Gnathosoma* (Figure 2A) — Gnathosoma length 62 (52–62), width 55 (53–60); cheliceral
stylets robust, length 16 (16–27); pharynx oval and well developed, length 41 (37–45), width
33 (24–34).

*Idiosoma* (Figure 2A) — Idiosoma ovoid and smooth, length 620 (458–670), width 482 (451–517), with bi-lobed protuberances anterolaterally and genital opening posteroventrally; prodorsal shield poorly sclerotised. Stigmata conspicuous and situated at anterior border of prodorsal shield, each with an elongate broad atrium with 4 distal tracheal branches, length of tracheal atrium about 2 times length of pharynx before enlarging and getting off multiple tracheal branches.

*Legs* — (Figure 2B) Only single pair of anterior legs; tarsus with one subunguinal seta (s) modified as terminal claw and both spine-like tectal setae *tc'* and *tc''* blunt; tibia with 1 spine-like seta *l'*; genu nude; femur with one prominent seta *l''* 18 (18–20).

Adult Male — (Figures 3-4)

*Gnathosoma* (Figure 3A) — length 33, width 29, dorsally with one pair of cheliceral seta *ch* 3 and ventrally with one pair of subcapitular seta *su* 5; cheliceral stylets 14; pharynx length 17, width 13; distances between gnathosomal setae: *ch-ch* 19, *su-su* 13.

*Idiosomal dorsum* (Figure 3A) — length 180, width 140; prodorsal plate (PrS) subtriangular, with smooth and pointed setae *v*₁ 4, *v*₂ 5 and attenuated *sc*₂ 86. Genital capsule placed in mid-dorsal idiosoma; pointed setae *c*₂ 118; distances between dorsal idiosomal setae: *v*₁-*v*₁ 24, *v*₂-*v*₂ 43, *sc*₂-*sc*₂ 56, *c*₂-*c*₂ 102.

*Idiosomal venter* (Figure 3B) — with apodemes I, II and presternal apodeme (appr) developed; apodemes II (ap2) not reaching to presternal apodeme; coxal fields I without setae *la*; coxal fields II with one pair of smooth and pointed setae 2*a* 6; coxal fields III fused and bearing only one pair of smooth and pointed setae 3*b* 16.

*Legs* (Figure 4). Leg I (Figure 4A) thicker and shorter than other legs; setal formula (number of solenidia in parentheses): 0-2-0-6-5(1); ambulacrum I with single small claw in sucker-like pad; tarsus with 2 distinctly blunt-ended eupathidial setae *tc'* and *tc''*, solenidion ω 7, prominent and digitiform, setae *s* and *pv''* spine-like; tibia with one spine-like seta *v'* 9 and 5 setiform setae, eupathidion *k* 4; femur with two setae, seta *l''* 5. Leg II (Figure 4B) with setal formula: 0-1-0-4-4; ambulacrum II with no claw but empodium well developed; tarsus with 3
<figure><img src="image_9.png" /><figcaption>**Figure 2** *Podapolipoides chorthippus* n. sp., (adult female). A. Dorsal view, B. Details of leg I (Paratype).</figcaption></figure>

spine-like setae *tc'*, *u'* and *pv''*, seta *tc''* attenuated, 42 long; tibia with two spine-like setae *v'* 11 and *l'* 8, seta *d* longer than *v''*; femur with one seta *l'*. Leg III (Figure 4C) with setal formula: 0-0-0-4-4; ambulacrum III without claw, empodium well developed; tarsus with 3 spine-like setae *tc'*, *u'* and *pv''*, seta *tc''* attenuate, 103 long; tibia with three spine-like setae *v'* 14, *v''* 13 and *l'* 14, seta *d* attenuate, 99 long.

**Larval female** — (Figures 5–6)

*Gnathosoma* (Figure 5A) — length 48 (58–60), width 70 (60–77); dorsally with one pair of cheliceral seta *ch* 16 (38–43) and ventrally with one pair of subcapitular seta *su* 8 (8–9); palpal setae *dFe* 12 (9–14); *dGe* 11 (11–16), all setae *ch, su, dFe, dFe* smooth and pointed;
<figure><img src="image_10.png" /><figcaption>**Figure 3** *Podapolipoides chorthippus* n. sp., male. A. Dorsal view. B. Ventral view.</figcaption></figure>

cheliceral stylets 63 (60–86); pharynx oval, length 17 (16–18), width 17 (14–19); distances between gnathosomal setae: *ch-ch* 34 (37–43), *dFe-dFe* 17 (17–23), *dGe-dGe* 8 (8–12), *su-su* 17 (17–23).

*Idiosomal dorsum* (Figure 5A) — oval, length 154 (154–182), width 136 (132–155); prodorsal plate (PrS) subrectangular, wider than long, smooth and pointed setae *v*₁ 4 (2–4), *v*₂ 7 (5–10), and attenuated setae *sc*₂ 93 (85–111); setae *sc*₂ extending beyond posterior margin of tergite D; tergite C surrounding anterior and lateral margins of tergite D, with only one pair of setae *c*₂ 18 (12–22); tergite D almost sub-circular, with one pair of needle-like setae *d* 7 (7–8); tergite EF subtriangular, with one pair of setae *f* 9 (9–18), indistinctly serrate (left side seta detached); tergite H with two pairs of setae, *h*₁ 207 (207–314) and *h*₂ 10 (10–19); setae *h*₁ whip-like and attenuated, *h*₂ slightly thick; distance between idiosomal setae: *v*₁-*v*₂ 58 (52–62), *v*₂-*v*₃ 89 (81–98), *sc*₂-*sc*₂ 52 (50–63), *c*₂-*c*₂ 125 (134–148), *d*-*d* 23 (22–32), *f*-*f* 47 (34–47), *h*₂-*h*₃ 14 (12–14).

*Idiosomal venter* (Figure 5B) — apodemes I (ap1) and II (ap2) lightly sclerotized but ap2 not reaching presternal apodeme (appr); coax fields I and II each bearing one pair of microsetae *1a* and *2a*, respectively; coax fields III separated from each other and from coax fields II; setae *3b* m (m-4), with striation between and around coax fields III.

*Legs* — (Figure 6), Leg I (Figure 6A) thicker than other legs; setal formula (number of solenidia in parentheses): 0-3-1-6-7(1); ambulacrum I with bifid small claw in sucker-like pad; tarsus with two distinctly blunt-ended eupathidial setae *tc'* and *tc''*, solenidion ω 7 (6–8) prominent and digitiform, seta *s* spine-like; tibia with eupathidion *k* 6 (6–9) adjacent to long
<figure><img src="image_11.png" /><figcaption>**Figure 4** *Podapolipoides chorthippus* n. sp., (male). (A-C). Legs I–III, respectively. Dorsal view, right legs.</figcaption></figure>

seta *d* 36 (34–46), setae *v''* and *l''* subequal; genu with one seta *v'*; femur with three setae, seta *l'* needle-like, seta *l''* 31 (34–46) extending beyond tip of leg and distinctly longer than seta *v''* 26 (31–35). Leg II (Figure 6B) with setal formula: 0-1-1-4-4; ambulacrum II without claw, empodium well-developed; tarsus with two spine-like setae *tc'* and *u'*, seta *pv''* needle-like; tibia with seta *l'* needle-like, rest of setae of the segment pointed; seta *d* 28 (27–39) subequal with seta *v''* 27 (21–27); genu and femur each with one seta *v'* and *l'*, respectively, *l'* needle-like. Leg III (Figure 6C) with setal formula: 0-0-1-4-4; ambulacrum III without claw, empodium well-developed; tarsus with two spine-like setae *tc'* and *u'*, seta *pv''* needle-like; tibia with seta *d* 30 (45–50), all setae of segment pointed; genu with one needle-like seta *v'*.

**Differential diagnosis**

The male of new species belongs to a cluster of species sharing setae *c*₂ 100-125 µm long (see the following key for related these species): *Podapolipoides faini* Husband, 1995, *P. locustanus* Lavoipierre, 1941, *P. alatus* Husband, 1990, *P. cochisensis* Husband, 1993 and *P. anacridii* Hajiqanbar and Joharchi, 2011. According to length of setae *sc*₂ (80-96), *P. chorthippus* (86) is similar to *P. faini* (80) and *P. cochisensis* (96) but differ from *P. cochisensis* by the length of setae 3*b* 16 (*vs* vestigial in *P. cochisensis*) and *P. faini* in the length of cheliceral stylets 14 (*vs* 8 in *P. faini*) and coxal seta 3*b* shorter than gnathosomal width (*vs* equal to gnathosomal width in *P. faini*) also coxal seta 2*a* shorter than 1/3 gnathosomal width (*vs* about 1/3 gnathosomal width in *P. faini*), and in *P. chorthippus* larvae setae *c*₂ is longer than *d* (*vs* *c*₂ is subequal to *d* in *P. faini*). Regarding to setae 1*a*, *P. chorthippus* n. sp. with no setae 1*a* is similar to *P. alatus* but differs from it by setae *sc*₂ reaching to beyond bases of setae *c*₂ (*vs* not reaching to bases
<figure><img src="image_12.png" /><figcaption>**Figure 5** *Podapolipoides chorthippus* n. sp., (larval female). A. Dorsal view. B. Ventral view. C. Seta *h*₁ enlarged. D. Seta *f* enlarged. E. Seta *h*₂ enlarged.</figcaption></figure>

of setae *c*₂ in *P. alatus*. Based on apodemes II, *P. chorthippus* n. sp. resembles *P. anacridii*, *P. faini* and *P. locustanus* but differs from *P. anacridii* by ap1 reaching appr (*vs* not reaching appr in *P. anacridii*) and differs from *P. locustanus* by setae *sc*₂ reaching beyond bases of setae *c*₂ (*vs* not reaching beyond bases of setae *c*₂ in *P. locustanus*). Among the congeners found in neighboring countries, it is similar to *P. jordani* Husband, 1992. However, male of the new species differs from that of *P. jordani* by *sc*₂ reaching beyond bases of setae *c*₂, ap2 not reaching appr, and length of *tc***"* 103 (*vs* *sc*₂ not reaching beyond bases of setae *c*₂, ap2 reaching appr, *tc***"* 156 in *P. jordani*. The remainder of the comparative measurements is summarized in Table 1.

Based on length of setae $v_1$ and $v_2$ ($v_1 > v_2$), larval females of the *P. chorthippus* n. sp. can be easily distinguished from all other species of the genus except *P. flechtmanni* Husband, 1993. Based on longer setae $v_2$ than $v_1$, and almost the same length of setae $h_1$, $sc_2$ and *f*, larval females of *P. chorthippus* are similar to those of *P. flechtmanni*. However, they differ in shape of setae *d*, *f*, $h_2$. In *P. chorthippus*, setae *d* are thin and pointed, *f* serrate, and $h_2$ slightly thickened (*vs* *d*, *f*, $h_2$ thick and minutely spine-like in *P. flechtmanni*); setae *c*₂ 12–22 at least two times longer in *P. chorthippus* n. sp. (*vs* 6 in *P. flechtmanni*); and coxal setae I and II microsetae (*vs* 2 in *P. flechtmanni*).

## Etymology

The new species is named based on the genus name of the grasshopper host (*Chorthippus*).
<figure><img src="image_13.png" /><figcaption>**Figure 6** *Podapolipoides chorthippus* n. sp., (larval female). (A-C). Legs I–III, respectively. Dorsal view, A. right leg, B-C. left legs.</figcaption></figure>

**Key to males of Podapolipoides spp. with setae c₂ 100-125 µm**

1. Seta 3b vestigial .................................................. *P. cochisensis*
   — Seta 3b not vestigial, setiform .................................... 2

2. Seta *sc₂* reaching bases of seta *c₂* or beyond .................................................... 3
   — Seta *sc₂* never reaching bases of seta *c₂* .................................................... 5

3. Coxal fields I without setae 1a .................................... *P. chorthippus* n. sp.
   — Coxal fields I with one pair of vestigial setae 1a .................................... 4

4. Femur I seta v" equal to or longer than seta l" .................................... *P. locustanus*
   — Femur I seta v" about half of the length of seta l" .................................... *P. fiani*

5. Setae 2a microsetae or vestigial .................................... *P. alatus*
   — Setae 2a not microseta or vestigial, setiform .................................... *P. anacridii*

**Discussion**

The genus *Chorthippus* is a new host record for parasitic mites of the genus *Podapolipoides*. It is a member of the subfamily Gomphocerinae (Orthoptera: Acrididae). According to the prevalence of *Podapolipoides* mites on orthopterans provided by Hajiqanbar and Joharchi (2011), 23 genera of Acrididae (22) and Pyrgomorphidae (1) are parasitized by this mite genus. Sarangi *et al.* (2012) described a new *Podapolipoides* from *Oxya* (Acrididae: Oxyinae), a grasshopper genus previously recorded as host of three species of *Podapolipoides*: *P. andrei*, *P. hopperae*
and *P. mohanasundarami* (see Husband 1995; Ramaraju & Mohanasundaram 1996; Ramaraju & Suresh 1999). Hitherto, only one genus of the subfamily Gomphocerinae was recorded as a host of *Podapolipoides*, i.e. *Orphulella* (Feldman-Muhsam and Havivi, 1973). Including the current study, Gomphocerinae includes two host genera (*Orphulella* and *Chorthippus*) of *Podapolipoides*. This extend the number of orthopteran host genera of *Podapolipoides* to 24 (23 belonging to Acrididae). Therefore, the prevalence of *Podapolipoides* mites on acridids

<table><caption>**Table 1** Comparison of selected maximum measurements of *P. chorthippus* n. sp. (Pch), *P. anacridii* (Pan), *P. faini* (Pf), *P. locustanus* (Pl), *P. alatus* (Pal) and *P. cochisensis* (Pc).</caption><thead><tr><th rowspan="2">Character</th><th colspan="6">Species</th></tr><tr><th>Pch</th><th>Pan</th><th>Pf</th><th>Pl</th><th>Pal</th><th>Pc</th></tr></thead><tbody><tr><td colspan="7"><strong>Adult female</strong></td></tr><tr><td>Idiosomal length</td><td>670</td><td>432</td><td>460</td><td>490</td><td>530</td><td>662</td></tr><tr><td>Idiosomal width</td><td>517</td><td>368</td><td>340</td><td>410</td><td>546</td><td>556</td></tr><tr><td>Gnathosomal width</td><td>62</td><td>70</td><td>40</td><td>48</td><td>56</td><td>47</td></tr><tr><td>Cheliceral stylet</td><td>27</td><td>33</td><td>18</td><td>22</td><td>29</td><td>27</td></tr><tr><td>Pharynx width</td><td>34</td><td>36</td><td>28</td><td>33</td><td>32</td><td>33</td></tr><tr><td>Femur I seta l'</td><td>20</td><td>20</td><td>14</td><td>19</td><td>15</td><td>15</td></tr><tr><td>Tarsus I seta tc'</td><td>4</td><td>7</td><td>5</td><td>5</td><td>5</td><td>5</td></tr><tr><td colspan="7"><strong>Male</strong></td></tr><tr><td>Idiosoma length</td><td>180</td><td>205</td><td>147</td><td>158</td><td>158</td><td>133</td></tr><tr><td>Idiosoma width</td><td>140</td><td>152</td><td>113</td><td>133</td><td>137</td><td>120</td></tr><tr><td>Gnathosomal width</td><td>29</td><td>29</td><td>23</td><td>28</td><td>29</td><td>27</td></tr><tr><td>Cheliceral stylet</td><td>14</td><td>13</td><td>8</td><td>8</td><td>10</td><td>13</td></tr><tr><td>Subcapitular seta su</td><td>5</td><td>6</td><td>5</td><td>5</td><td>4</td><td>2</td></tr><tr><td>v<sub>2</sub></td><td>5</td><td>8</td><td>5</td><td>7</td><td>3</td><td>5</td></tr><tr><td>c<sub>2</sub></td><td>118</td><td>125</td><td>104</td><td>123</td><td>113</td><td>123</td></tr><tr><td>sc<sub>2</sub></td><td>86</td><td>65</td><td>80</td><td>105</td><td>74</td><td>96</td></tr><tr><td>3b</td><td>16</td><td>27</td><td>23</td><td>37</td><td>29</td><td>v</td></tr><tr><td>Tibia I seta k</td><td>4</td><td>5</td><td>7</td><td>8</td><td>5</td><td>5</td></tr><tr><td>Tibia I seta v'</td><td>9</td><td>10</td><td>9</td><td>11</td><td>8</td><td>26</td></tr><tr><td>Tibia III seta d</td><td>99</td><td>125</td><td>96</td><td>118</td><td>133</td><td>107</td></tr><tr><td>Tibia III seta v'</td><td>14</td><td>15</td><td>13</td><td>17</td><td>18</td><td>13</td></tr><tr><td>Tarsus III seta tc"</td><td>103</td><td>128</td><td>104</td><td>152</td><td>142</td><td>125</td></tr><tr><td colspan="7"><strong>Larval female</strong></td></tr><tr><td>Idiosomal length</td><td>182</td><td>235</td><td>146</td><td>179</td><td>176</td><td>143</td></tr><tr><td>Idiosomal width</td><td>155</td><td>201</td><td>122</td><td>173</td><td>143</td><td>129</td></tr><tr><td>Gnathosomal width</td><td>77</td><td>77</td><td>52</td><td>62</td><td>52</td><td>48</td></tr><tr><td>Cheliceral stylet</td><td>86</td><td>99</td><td>60</td><td>66</td><td>68</td><td>52</td></tr><tr><td>Cheliceral seta ch</td><td>43</td><td>42</td><td>28</td><td>34</td><td>25</td><td>28</td></tr><tr><td>dFe</td><td>14</td><td>18</td><td>13</td><td>12</td><td>10</td><td>5</td></tr><tr><td>v<sub>1</sub></td><td>4</td><td>20</td><td>12</td><td>15</td><td>17</td><td>10</td></tr><tr><td>v<sub>2</sub></td><td>10</td><td>11</td><td>10</td><td>10</td><td>7</td><td>7</td></tr><tr><td>d</td><td>8</td><td>24</td><td>15</td><td>18</td><td>22</td><td>15</td></tr><tr><td>Distance d-d</td><td>32</td><td>54</td><td>39</td><td>40</td><td>35</td><td>37</td></tr><tr><td>f</td><td>18</td><td>24</td><td>15</td><td>16</td><td>24</td><td>14</td></tr><tr><td>h<sub>1</sub></td><td>314</td><td>400</td><td>415</td><td>428</td><td>497</td><td>385</td></tr><tr><td>h<sub>2</sub></td><td>19</td><td>25</td><td>18</td><td>15</td><td>20</td><td>12</td></tr><tr><td>Femur I seta l"</td><td>46</td><td>51</td><td>39</td><td>42</td><td>49</td><td>38</td></tr><tr><td>Tibia I seta k</td><td>9</td><td>8</td><td>5</td><td>9</td><td>8</td><td>7</td></tr><tr><td>Tibia III seta d</td><td>50</td><td>70</td><td>51</td><td>51</td><td>57</td><td>29</td></tr></tbody></table>
and pyrgomorphids subfamilies is updated as follows: Oedipodinae (38.6%), Cyrtacanthacridinae (27.2%), Acridinae (11.4%), Oxyinae (9.1%), and Melanoplinae, Gomphocerinae, and Pyrgomorphinae each with 4.5%.

## Acknowledgments

We are grateful to Dr. Mustafa Ünal (Abant İzzet Baysal Üniversitesi, Turkey) for help in identifying the host grasshopper. Also, we are thankful to anonymous reviewers for their useful comments and suggestions which highly improved the paper.

## References

* Feldman-Muhsam B., Havivi Y. 1973. *Podapolipus (Podapolipoides) peruvensis*, a new species from Peru (Acarina, Podapolipodidae). Acarologia, 15: 716-723.

* Hajiqanbar H., Joharchi O. 2011. World distribution and host range of *Podapolipoides* spp. (Acari: Heterostigmatina: Podapolipidae), with the description of a new species. Syst. Parasitol., 78(2): 151-162. https://doi.org/10.1007/s11230-010-9284-5

* Husband R.W. 1995. Four new species of *Podapolipoides* (Acari: Podapolipidae), ectoparasitic on grasshoppers (Orthoptera: Acrididae) from Africa. Int. J. Acarol., 21(1): 47-61. https://doi.org/10.1080/01647959508684042

* Husband R.W., O'Connor B.M. 2014. A new genus and species of Podapolipidae (Acari: Heterostigmata) parasitic on *Physonota alutacea* (Boheman) (Coleoptera: Chrysomelidae; Cassidinae) in Mexico and Central America. Syst. Appl. Acarol., 19(4): 435-446. https://doi.org/10.11158/saa.19.4.7

* Husband R.W., Zhang Z.-Q. 2002. *Wetapolipus jamiesoni* gen. nov., spec. nov. (Acari: Podapolipidae), an ectoparasite of the mountain stone weta, *Hemideina maori* (Orthoptera: Anostostomatidae) from New Zealand. Zootaxa, 125(1): 1-12. https://doi.org/10.11646/zootaxa.125.1.1

* Husband R.W., O'Connor B.M., Ochoa R. 2005. Two new species of *Orthapolipus* (Acari: Podapolipidae), parasites of Central American Tettigoniidae (Hexapoda: Orthoptera), with a rediagnosis of the genus. Int. J. Acarol., 31: 355-362. https://doi.org/10.1080/01647950508683675

* Lindquist E.E. 1986. The world genera of Tarsonemidae (Acari: Heterostigmata): a morphological, phylogenetic, and systematic revision, with a reclassification of family-group taxa in the Heterostigmata. Mem. Ent. Soc. Can., 118(S136): 1-517. https://doi.org/10.4039/entm118136fv

* Lindquist E.E., Sidorchuk E.A. 2019. A new species of *Podapolipus* (Acari: Heterostigmata: Podapolipidae) from an Australian gryllacridid cricket (Orthoptera), with keys to orthopteran-associated species of the genus. Zootaxa, 4647(1): 115-133. https://doi.org/10.11646/zootaxa.4647.1.11

* Majidi M., Hajiqanbar H., Saboori A. 2019. A new species of *Podapolipus* (Acari: Podapolipidae) from Iran: the first host record of a podapolipid mite parasitizing the widespread beetle *Trachyderma hispida* (Coleoptera: Tenebrionidae). Biologia, 74(6): 675-682. https://doi.org/10.2478/s11756-019-00206-1

* Mortazavi A., Hajiqanbar H., Lindquist E.E. 2018. A new family of mites (Acari: Prostigmata: Raphigathina), highly specialized subelytral parasites of dytiscid water beetles (Coleoptera: Dytiscidae: Dytiscinae). Zool. J. Lin. Soc., 184(3): 695-749. https://doi.org/10.1093/zoolinnean/zlx113

* Ostovan H., Saboori A. 1999. Some mites of the families Podapolipidae, Acarophenacidae and Podocinidae in Iran. J. Agric. Sci. Islam. Azad Univ., 5: 81-90.

* Rahmatzaei B., Hajiqanbar H., Mortazavi A., Husemann M. 2021. Global distribution and host range of the endoparasitic mite genus *Locustacarus* (Acari: Podapolipidae) with description of a new species from Iran parasitizing grasshoppers (Orthoptera: Acrididae). Syst. Parasitol., 98(4): 487-501. https://doi.org/10.1007/s11230-021-09991-3

* Ramaraju K., Mohanasundaram M. 1996. New species of *Podapolipus*, *Podapolipoides* and *Tarsopolipus* (Acari: Podapolipidae) from South India. Int. J. Acarol., 22: 33-41. https://doi.org/10.1080/01647959608684079

* Ramaraju K., Suresh S. 1999. A new species of *Podapolipoides* (Acari: Podapolipidae) from Tamil Nadu, India. Int. J. Acarol., 25: 195-199. https://doi.org/10.1080/01647959908684153

* Sarangi P., Biswas H., Gupta S.K., Saha G.K. 2012. Description of two new species of ectoparasitic mites of *Podapolipus* and *Podapolipoides* (Acari: Podapolipidae) on *Oxya* sp. from West Bengal, India. J. Asia. Pac. Entomol., 15: 192-195. https://doi.org/10.1016/j.aspen.2011.10.007

* Seeman O.D. 2021. Contrasting species diversification of *Eutarsopolipus* (Acariformes: Podapolipidae) on *Castelnaudia* and *Notonomus* (Coleoptera: Carabidae). Zootaxa, 4971(1): 1-74. https://doi.org/10.11646/zootaxa.4971.1.1

* Walter D.E., Lindquist E.E., Smith I.M., Cook D.R., Krantz G.W. 2009. Order Trombidiformes. In: Krantz G.W., Walter D.E, (Eds.), A manual of acarology (3rd ed.), Lubbock: Texas Tech University Press. p. 233-420.