# Whole-Genome Sequences of a Cluster of 14 Unidentified Related Veillonella sp. Strains from Human Clinical Samples and Type Strains of 3 Veillonella Validated Species

Fabien Aujoulat, Michel R. Popoff, Laure Diancourt, Alexis Criscuolo, Hélène Jean-Pierre, Estelle Jumas-Bilak, Hélène Marchandin

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Fabien Aujoulat, Michel R. Popoff, Laure Diancourt, Alexis Criscuolo, Hélène Jean-Pierre, et al. Whole-Genome Sequences of a Cluster of 14 Unidentified Related Veillonella sp. Strains from Human Clinical Samples and Type Strains of 3 Veillonella Validated Species. Microbiology Resource Announcements, 2019, 8 (12), 10.1128/MRA.01743-18. pasteur-02448615

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# Whole-Genome Sequences of a Cluster of 14 Unidentified Related *Veillonella* sp. Strains from Human Clinical Samples and Type Strains of 3 *Veillonella* Validated Species

Fabien Aujoulat,ª Michel R. Popoff,ᵇ Laure Diancourt,ᵇ Alexis Criscuolo,ᶜ Hélène Jean-Pierre,ᵈ Estelle Jumas-Bilak,ᵉ Hélène Marchandinf

**ABSTRACT** We report 17 draft genomes for 14 unidentified *Veillonella* sp. strains closely related in 16S rRNA gene-based phylogeny and type strains of 3 *Veillonella* species with the aims of deciphering relationships between related species, evaluating the accuracy of current thresholds for species delineation, and robustly describing new species in the genus.

Veillonella spp. are anaerobic Gram-negative cocci and important representatives of the microbiota of humans and animals. Currently, 14 species in the genus Veillonella are validly described. In the absence of discriminative phenotypic characteristics, their identification requires molecular-based methods. However, all species are not discriminated by 16S rRNA gene (rrs) analysis because several pairs of species are closely related (≥99% of identical rrs nucleotides), such as V. denticariosi and V. rodentium, V. ratti and V. criceti, V. ratti and V. seminalis, and V. dispar and V. parvula (1-4). In addition, intrachromosomal heterogeneity between rrs copies (up to 1.43%) and intraspecific rrs variability that may surpass interspecific variability have been demonstrated in this genus (3, 5). This impairs the 16S rRNA gene-based identification of closely related species and suggests that applying the proposed revised threshold for a new species description, which is less than 98.7% of 16S rRNA gene identity, may not be adapted to the genus Veillonella (6). Therefore, molecular-based identification methods based on housekeeping genes such as dnaK, rpoB, and gltA were successively developed and used for the description of novel species in addition to or without associated DNA-DNA hybridization (DDH), which is the reference method for novel species description (2, 4).

We present draft genome sequences of 14 human clinical isolates and type strains of 3 *Veillonella* species that were not available at the time of our study. Isolates were recovered from human clinical samples from 14 patients attending the University Hospital in Montpellier, France. The study was approved by the institutional review board of the Nîmes University Hospital under the approval number 19.01.07. All strains were cultured on Columbia sheep blood agar (bioMérieux) at 37°C in an anaerobic jar with the AnaeroGen system (Oxoid Unipath) for 2 to 5 days (1, 4).

DNA was extracted with the MasterPure DNA purification kit (Epicentre Biotechnologies). Libraries were constructed with the Nextera XT DNA library preparation kit (Illumina, San Diego, CA) and sequenced on a NextSeq 500 instrument with a 2 × 150-bp paired-end protocol (on average, 1,372,528 read pairs per sample and

**Citation** Aujoulat F, Popoff MR, Diancourt L, Criscuolo A, Jean-Pierre H, Jumas-Bilak E, Marchandin H. 2019. Whole-genome sequences of a cluster of 14 unidentified related *Veillonella* sp. strains from human clinical samples and type strains of 3 *Veillonella* validated species. Microbiol Resour Announc 8:e01743-18. https://doi.org/10.1128/MRA.01743-18.

**Editor** John J. Dennehy, Queens College

**Copyright** © 2019 Aujoulat et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

Address correspondence to Hélène Marchandin, helene.marchandin@umontpellier.fr.

**Received** 8 January 2019

**Accepted** 20 February 2019

**Published** 21 March 2019

<footnote>ªHydroSciences Montpellier, CNRS, IRD, Université de Montpellier, Montpellier, France</footnote>

<footnote>ᵇCentre National de Référence des bactéries anaérobies et du botulisme, Institut Pasteur, Paris, France</footnote>

<footnote>ᶜInstitut Pasteur-Bioinformatics and Biostatistics Hub-C3BI, USR 3756 IP CNR, Paris, France</footnote>

<footnote>ᵈCHU Montpellier, Laboratoire de Bactériologie, Montpellier, France</footnote>

<footnote>ᵉHydroSciences Montpellier, CNRS, IRD, Université de Montpellier, CHU Montpellier, Laboratoire d'Ecologie Microbienne Hospitalière, Montpellier, France</footnote>

<footnote>fHydroSciences Montpellier, CNRS, IRD, Université de Montpellier, CHU Nîmes, Nîmes, France</footnote>
<figure><img src="image_2.png" /><figcaption>TABLE 1 Characteristics of the 17 Veillonella sp. draft genome sequences<sup>a</sup></figcaption></figure>

<table><thead><tr><th>Species</th><th>Strain</th><th>Accession no.</th><th>BioSample no.</th><th>No. of paired-end reads</th><th>Coverage (x)</th><th>Genome size (bp)</th><th>No. of contigs</th><th>N<sub>50</sub> (bp)</th><th>G+C content (%)</th></tr></thead><tbody><tr><td>V. ratti</td><td>ATCC 17746<sup>T</sup></td><td>RQUX00000000</td><td>SAMN10465589</td><td>1,163,748</td><td>156</td><td>2,236,286</td><td>49</td><td>95,000</td><td>42.8</td></tr><tr><td>V. caviae</td><td>DSM 20738<sup>T</sup></td><td>RQUY00000000</td><td>SAMN10465590</td><td>1,391,318</td><td>211</td><td>1,963,997</td><td>57</td><td>83,095</td><td>38.4</td></tr><tr><td>V. seminalis</td><td>ADV 4313.2<sup>T</sup> = CIP 107810T</td><td>RQUZ00000000</td><td>SAMN10465591</td><td>1,399,442</td><td>184</td><td>2,252,690</td><td>27</td><td>152,260</td><td>42.2</td></tr><tr><td>Veillonella sp.</td><td>CHU110 = 14/09/02-B-6149</td><td>ROVA00000000</td><td>SAMN10465592</td><td>973,834</td><td>155</td><td>1,881,921</td><td>43</td><td>107,364</td><td>39.4</td></tr><tr><td>Veillonella sp.</td><td>Va140 = CHU594 = 03/11/05-B-4237</td><td>RQVB00000000</td><td>SAMN10465593</td><td>1,206,819</td><td>199</td><td>1,822,704</td><td>23</td><td>147,662</td><td>39.3</td></tr><tr><td>Veillonella sp.</td><td>Va138 = CHU732 = 06/09/06-B-3252</td><td>RQVC00000000</td><td>SAMN10465594</td><td>740,997</td><td>112</td><td>1,972,307</td><td>42</td><td>104,904</td><td>39.5</td></tr><tr><td>Veillonella sp.</td><td>Va143 = CHU740 = 09/10/06-B-1508</td><td>RQVD00000000</td><td>SAMN10465595</td><td>1,518,558</td><td>240</td><td>1,893,839</td><td>35</td><td>130,014</td><td>39.3</td></tr><tr><td>Veillonella sp.</td><td>3891 = 12/03/14-B-3204</td><td>RQVE00000000</td><td>SAMN10465596</td><td>1,295,195</td><td>205</td><td>1,890,765</td><td>38</td><td>161,698</td><td>39.4</td></tr><tr><td>Veillonella sp.</td><td>3913 = 09/03/14-B-7013</td><td>RQVF00000000</td><td>SAMN10465597</td><td>698,249</td><td>118</td><td>1,770,519</td><td>15</td><td>394,883</td><td>39.4</td></tr><tr><td>Veillonella sp.</td><td>3627 = CHU4040 = 99141203627</td><td>RQVG00000000</td><td>SAMN10465598</td><td>1,308,145</td><td>226</td><td>1,738,782</td><td>34</td><td>132,446</td><td>39.3</td></tr><tr><td>Veillonella sp.</td><td>CHU3735 = CNR 79/14 = 14/01/14-B-2378</td><td>RQVH00000000</td><td>SAMN10465599</td><td>1,123,247</td><td>190</td><td>1,776,932</td><td>51</td><td>93,431</td><td>39.4</td></tr><tr><td>Veillonella sp.</td><td>3960 = CHU4076 = 99141423960</td><td>RQVI00000000</td><td>SAMN10465600</td><td>746,487</td><td>120</td><td>1,860,088</td><td>69</td><td>60,297</td><td>39.7</td></tr><tr><td>Veillonella sp.</td><td>3310 = CHU5981 = 99162773310</td><td>RQVJ00000000</td><td>SAMN10465601</td><td>1,640,876</td><td>258</td><td>1,870,378</td><td>16</td><td>311,068</td><td>39.6</td></tr><tr><td>Veillonella sp.</td><td>Va137 = 19/07/06-3404</td><td>RQVK00000000</td><td>SAMN10465602</td><td>1,966,481</td><td>310</td><td>1,902,403</td><td>36</td><td>128,606</td><td>39.6</td></tr><tr><td>Veillonella sp.</td><td>Va139 = 10/01/06-3360</td><td>RQVL00000000</td><td>SAMN10465603</td><td>2,226,458</td><td>303</td><td>2,172,276</td><td>76</td><td>67,981</td><td>39.5</td></tr><tr><td>Veillonella sp.</td><td>Va141 = 20/06/06-2457</td><td>RQVM00000000</td><td>SAMN10465604</td><td>2,034,756</td><td>333</td><td>1,806,031</td><td>12</td><td>229,161</td><td>39.2</td></tr><tr><td>Veillonella sp.</td><td>Va142 = 08/06/05-3381</td><td>RQVN00000000</td><td>SAMN10465605</td><td>1,898,363</td><td>281</td><td>1,969,382</td><td>66</td><td>75,004</td><td>39.1</td></tr></tbody></table>

<footnote><sup>a</sup> BioProject number, PRJNA506647.</footnote>
<figure><img src="image_3.png" /></figure>

~212× sequencing depth; Table 1). All sequenced paired-end reads were clipped and trimmed with AlienTrimmer v. 0.4.0 (7), corrected with Musket v. 1.1 (8), and subjected to a digital normalization procedure with khmer v. 1.3 (9). For each sample, the remaining processed reads were assembled and scaffolded with SPAdes v. 3.11.0 (10). All programs were used with their default settings. Strains and characteristics of their whole-genome sequences (WGSs) are presented in Table 1.

Because genomic sequencing is being progressively incorporated into the taxonomy of *Bacteria* (11) and considering that it was not used before in the genus *Veillonella*, taxogenomic metrics such as *in silico* DDH, average nucleotide identity, and phylogenomics will be valuable for (i) deciphering intrageneric relationships between related species and between strains affiliated with a species or of unknown taxonomic status, (ii) evaluating the accuracy of current thresholds for species delineation, (iii) supporting reappraisal of the taxonomy, (iv) robustly describing new species, and (v) proposing minimal standards for description of new species in the genus *Veillonella*. Besides taxonomic purposes, WGS will also allow the characterization of clinically relevant gene content, particularly virulence and antibiotic resistance genes, in *Veillonella* spp. considered opportunistic human pathogens.

**Data availability.** This whole-genome shotgun project was deposited at DDBJ/ENA/GenBank under the accession numbers RQUX00000000 to RQVN00000000 as listed in Table 1 (BioProject number PRJNA506647; BioSample numbers SAMN10465589 to SAMN10465605). The versions described in this paper are versions RQUX01000000 to RQVN01000000. The Sequence Read Archive accession numbers are SRX5189907 to SRX5189923.

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