# Teasing apart the contributions of the different mechanisms involved in Myzus persicae resistance to neonicotinoid

Claire Mottet, Laetitia Caddoux, Séverine Fontaine, Christophe Plantamp, Chris Bass, Benoit Barrès

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Claire Mottet, Laetitia Caddoux, Séverine Fontaine, Christophe Plantamp, Chris Bass, et al.. Teasing apart the contributions of the different mechanisms involved in Myzus persicae resistance to neonicotinoid. Resistance 2024, Sep 2024, Rothamsted, United Kingdom. anses-04846176

HAL Id: anses-04846176

https://anses.hal.science/anses-04846176v1

Submitted on 19 Dec 2024

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# Teasing apart the contributions of the different mechanisms involved in *Myzus persicae* resistance to neonicotinoid

C. Mottet<fnref n="1" />, L. Caddoux<fnref n="1" />, S. Fontaine<fnref n="1" />, C. Plantamp<fnref n="1" />, C. Bass<fnref n="2" />, B. Barrès<fnref n="1" />

## CONTEXT

Numerous resistance mechanisms exist. They fall into two broad categories: Target Site Resistance (TSR) and Non-Target Site Resistance (NTSR) mechanisms.

These mechanisms may be present alone or in combination within an individual. Their interaction and relative importance, however, remain poorly studied.

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TSR

(eg: target modification)

<figure><img src="image_3.png" /></figure>

NTSR

(eg: detoxification)

## BIOLOGICAL MODEL

* *Myzus persicae* (Sulzer 1776)

* Generalist (>50 crop species)

* Evolved resistance against neonicotinoids

* both TSR (R81T) and NTSR (P450 overexpression) mechanisms described

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## QUESTIONS

* What is the contribution of the different mechanisms in the resistance phenotypes?

* What is the importance of the interaction between mechanisms in the resistance phenotypes?

* Is it possible to infer the order of evolution of resistance mechanisms?

## BIOLOGICAL MATERIAL

* 23 *Myzus persicae* clonal lineages

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* Sampled from 3 host plants

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## THIACLOPRID BIOASSAYS

Artificial feeding bioassay - AP3 medium + 10 to 40960µg/L thiacloprid

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With or without PBO (4mg/cm<fnref n="2" />) sprayed with Potter tower

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Dead or alive assessment 48 h after exposure

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Dose-response curve analyses 'drc' in R

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## GENOTYPING

* Neutral microsatellite genotyping (14 markers)

* Target site mutation analyses (sanger sequencing)

* P450 genomic copy number (qPCR)

* P450 gene expression (RT-qPCR)

## DATA ANALYSIS

* Correlation between LC50 with or without PBO

* Dominance level of TSR mutation

* Statistical modelling of resistance phenotype

* Independent effect of TSR on phenotype

* Independent effect of NTSR on phenotype

### LC50 / LC50.PBO

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### DETOXIFICATION COVARIATES

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## RAW RESULTS

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### TSR EFFECT

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### NTSR EFFECT

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## CONCLUSIONS

* Significant correlation between P450 expression and thiacloprid LC50

* P450 expression better predictor of thiacloprid LC50 than P450 copy number

* Significant synergy between TSR and NTSR

* Contribution of P450 expression highest in TSR resistant genotypes → point towards NTSR predating TSR in the case of *M. persicae* resistance against neonicotinoids

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RÉPUBLIQUE

FRANÇAISE

Liberté

Égalité

Fraternité

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<footnote n="1">¹Université de Lyon, Anses, INRAE, USC CASPER, Lyon France</footnote>

<footnote n="2">²Centre for Ecology and Conservation, University of Exeter, Penryn, UK</footnote>