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**How to cite this article:** EFSA (European Food Safety Authority), Hernandez-Jerez, A., Coja, T., Paparella, M., Price, A., Henri, J., Focks, A., Louisse, J., Terron, A., Binaglia, M., Guajardo, I. M., Mangas, I., Guajardo, I. M., Ferreira, L., Kardassi, D., De Lentdecker, C., Molnar, T., & Vianello, G. (2024). Statement on the toxicological properties and maximum residue levels of acetamiprid and its metabolites. *EFSA Journal*, 22(5), e8759. https://doi.org/10.2903/j.efsa.2024.8759
# APPENDIX A

## Used compound codes

<table><thead><tr><th>Code/trivial name<sup>a</sup></th><th>IUPAC name/SMILES notation/InChiKey<sup>b</sup></th><th>Structural formula<sup>c</sup></th></tr></thead><tbody><tr><td>Acetamiprid</td><td>(1E)-N'-[(6-chloro-3-pyridyl)methyl]-N'-cyano-N-methylacetimidamide<br>Clc1ccc(CN(C)C(\C)=N\C#N)cn1<br>WCXDHFDTOYPNIE-RIYZIHGNSA-N</td><td><img src="acetamiprid Structural formula.png" alt="Acetamiprid chemical structure"></td></tr><tr><td>N-desmethyl-acetamiprid<br>(IM-2-1)</td><td>(1E)-N'-[(6-chloropyridin-3-yl)methyl]-N'-cyanoethanimidamide<br>Clc1ccc(CNC(\C)=N\C#N)cn1<br>AYEAUPRZTZWBBF-UHFFFAOYSA-N</td><td><img src="N-desmethyl-acetamiprid (IM-2-1) Structural formula.png" alt="N-desmethyl-acetamiprid (IM-2-1) chemical structure"></td></tr><tr><td>IM-1-4</td><td>1-(6-chloropyridin-3-yl)-N-methylmethanamine<br>Clc1ccc(CNC)cn1<br>XALCOJXGWJXWBL-UHFFFAOYSA-N</td><td><img src="IM-1-4 Structural formula.png" alt="IM-1-4 chemical structure"></td></tr><tr><td>IM-1-5</td><td>N'-[(6-chloropyridin-3-yl)methyl]-N-methylethanimidamide<br>Clc1ccc(CN(C)C(C)=N)cn1<br>JHZWQGRBAHJYIZ-UHFFFAOYSA-N</td><td><img src="IM-1-5 Structural formula.png" alt="IM-1-5 chemical structure"></td></tr><tr><td>6-chloronicotinic acid<br>(IC-0)</td><td>6-chloropyridine-3-carboxylic acid<br>OC(=O)c1cnc(Cl)cc1<br>UAWMVMPAYRWUFX-UHFFFAOYSA-N</td><td><img src="6-chloronicotinic acid (IC-0) Structural formula.png" alt="6-chloronicotinic acid (IC-0) chemical structure"></td></tr><tr><td>IC-0-methyl ester</td><td>methyl 6-chloropyridine-3-carboxylate<br>Clc1ccc(cln1)C(=O)OC<br>RMEDXVIWDFLGES-UHFFFAOYSA-N</td><td><img src="IC-0-methyl ester Structural formula.png" alt="IC-0-methyl ester chemical structure"></td></tr><tr><td>IM-0</td><td>(6-chloropyridin-3-yl)methanol<br>OCc1cnc(Cl)cc1<br>GOXYBEXWMJZLJB-UHFFFAOYSA-N</td><td><img src="IM-0 Structural formula.png" alt="IM-0 chemical structure"></td></tr><tr><td>IM-0 (gly conjugate)</td><td>(2R,3R,4S,5S,6R)-2'-[(6-chloropyridin-3-yl)methoxy]-6-(hydroxymethyl)<br>oxane-3,4,5-triol<br>Clc1ccc(CO[C@H]2O[C@H](CO)[C@H](O)[C@H](O)[C@H]2O)cn1<br>ZRRXFGLNJBNGQI-DVYMNCLGSA-N</td><td><img src="IM-0 (gly conjugate) Structural formula.png" alt="IM-0 (gly conjugate) chemical structure"></td></tr><tr><td>IS-2-1 (N-<br>cyanoacetamidine<br>derivative)</td><td>(1E)-N'-cyanoethanimidamide<br>C/C(N)=N\C#N<br>KKZFHAKALPLYLL-UHFFFAOYSA-N</td><td><img src="IS-2-1 (N-<br>cyanoacetamidine<br>derivative) Structural formula.png" alt="IS-2-1 (N-<br>cyanoacetamidine derivative) chemical structure"></td></tr><tr><td>IS 1-1</td><td>(1E)-N'-cyano-N-methylethanimidamide<br>C/C(=N\C#N)NC<br>HGUZMABFJZHYJY-UHFFFAOYSA-N</td><td><img src="IS 1-1 Structural formula.png" alt="IS 1-1 chemical structure"></td></tr></tbody></table>
<table><thead><tr><th>Code/trivial name<sup>a</sup></th><th>IUPAC name/SMILES notation/InChiKey<sup>b</sup></th><th>Structural formula<sup>c</sup></th></tr></thead><tbody><tr><td>IM 1-2</td><td>(1E)-N'-carbamoyl-N-[(6-chloropyridin-3-yl)methyl]-N-methylethanimidamide<br>Clc1ccc(CN(C)C(\C)=N\C(N)=O)cn1<br>ISBUGOZWWNMPPC-VGOFMYFVSA-N</td><td><img src="chemical_structure_1.png" alt="Structural formula of (1E)-N'-carbamoyl-N-[(6-chloropyridin-3-yl)methyl]-N-methylethanimidamide"></td></tr><tr><td>IM-1-3</td><td>N-[(6-chloropyridin-3-yl)methyl]-N-methylacetamide<br>Clc1ccc(CN(C)C(C)=O)cn1<br>FFKDBDOYQUWIEU-UHFFFAOYSA-N</td><td><img src="chemical_structure_2.png" alt="Structural formula of N-[(6-chloropyridin-3-yl)methyl]-N-methylacetamide"></td></tr><tr><td>IM-2-3</td><td>N-[(6-chloropyridin-3-yl)methyl]acetamide<br>Clc1ccc(CNC(C)=O)cn1<br>PKLYKZAYVXYVQX-UHFFFAOYSA-N</td><td><img src="chemical_structure_3.png" alt="Structural formula of N-[(6-chloropyridin-3-yl)methyl]acetamide"></td></tr></tbody></table>

<footnote><sup>a</sup>The name in bold is the name used in the statement.</footnote>

<footnote><sup>b</sup>ACD/Name 2021.1.3 ACD/Labs 2021.1.3 (File Version N15E41, Build 123232, 7 July 2021).</footnote>

<footnote><sup>c</sup>ACD/ChemSketch 2021.1.3 ACD/Labs 2021.1.3 (File Version C25H41, Build 123835, 28 August 2021).</footnote>
APPENDICES 1–23

**Appendices 1–23 can be found in the online version of this output (‘Supporting information’ section): https://doi.org/10.2903/j.efsa.2024.8759**

**Appendix 1.** Protocol of the toxicological assessment

**Appendix 2.** Overview studies extension of the mandate PAN letter

**Appendix 3.** Outcome of the risk of bias (ROB) for in vivo (A), in vitro (B) and human observational (HOS) studies in the body of evidence (BOE) from public literature

**Appendix 4.** PAN letter study zebrafish data extraction and appraisal

**Appendix 5.** Data extraction DNT studies EFSA systematic literature

**Appendix 6.** RoB DNT studies EFSA systematic literature

**Appendix 7.** Rodent DNT TG uncertainty analysis

**Appendix 8.** Toxicological assessment IM-2-1 metabolite peer review

**Appendix 8a.** Peer review meeting report TC 119 acetamiprid – mammalian toxicity

**Appendix 9.** Updated critical appraisal tool (CAT) human biomonitoring studies

**Appendix 10.** Approach kinetic data collection and appraisal of kinetic studies

**Appendix 11.** Results appraisal human biomonitoring (HBM) studies exposure

**Appendix 12.** Results kinetic data collection and appraisal of kinetic studies

**Appendix 13.** Data extraction kinetic studies

**Appendix 14.** Results appraisal kinetic studies

**Appendix 15.** Considerations data requirements to develop acetamiprid PBK model

**Appendix 16.** Overview of the monitoring data collected for ToR2a

**Appendix 17.** Input values used for consumer risk assessment (scenario 1, 2, 3) and for livestock dietary burden

**Appendix 18.** GAP overview file (all GAPs received and assessed in ToR 3)

**Appendix 19.** GAP table (fall-back GAPs considered for the assessment of fall-back MRLs)

**Appendix 20.** PRIMo scenario 1

**Appendix 21.** PRIMo scenario 2

**Appendix 22.** PRIMo scenario 3

**Appendix 23.** Livestock dietary burden