# Determining the prevalence of geminiviruses in vegetable crops in the Mediterranean

Zineb Belabess, Monia Mnari Hattab, Ghandi Anfoka, Martine Granier, Gian Paolo Accotto, Miassar Altaleb, Massimiliano Ballardini, Umberto Bernardo, Salvatore Davino, Meryem Ennmily, et al.

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## To cite this version:

Zineb Belabess, Monia Mnari Hattab, Ghandi Anfoka, Martine Granier, Gian Paolo Accotto, et al. Determining the prevalence of geminiviruses in vegetable crops in the Mediterranean. 31. International Horticultural Congress (IHC2022): International Symposium on Water: a Worldwide Challenge for Horticulture!, ISHS, Aug 2022, Angers, France. 1 p. hal-05179806

HAL Id: hal-05179806

https://hal.science/hal-05179806v1

Submitted on 23 Jul 2025

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Title:

Determining the prevalence of geminiviruses in vegetable crops in the Mediterranean

Author(s):

Zineb Belabess, Plant Protection Laboratory., Regional Center of INRA, Oujda, Morocco;
belabess.zineb@gmail.com (co-author)

Monia Mnari-Hattab, Institut National de la Rech. Agronomique, INRAT, 1004 El Menzah, Tunis,
Tunisia; mmnari@gmail.com (co-author)

Ghandi Anfoka, Al-Balqa Applied University, 19117 Al-Salt, Jordan; anfoka@bau.edu.jo (co-author)

Martine Granier, Campus International de Baillarguet, 34398 Hérault Montpellier Cedex 5, France;
martine.granier@cirad.fr (co-author)

Gian Paolo Accotto, CNR-IPSP National Research Council, Institute for Sustainable Plant Protection,
10135 Torino, Italy; Gianpaolo.Accotto@ipsp.cnr.it (co-author)

Miassar Altaleb, Al-Balqa Applied University, 19117 Al-Salt, Jordan; miassaraltaleb@bau.edu.jo (co-author)

Massimiliano Ballardini, ESASEM S.p.A., 37052 Casaleone VR, Italy; mballardini@esasem.com (co-author)

Umberto Bernardo, CNR-IPSP National Research Council, Institute for Sustainable Plant Protection,
80055 Portici NA, Italy; umberto.bernardo@ipsp.cnr.it (co-author)

Salvatore Davino, Depart. of Agric, Food and Forest Science, University of Palermo, Palermo, Italy;
salvatore.davino@unipa.it (co-author)

Meryem Ennmily, Domaine Margau SARL, Douar Rjila B.P. 448, 87 052 AIT AMIRA, Morocco;
meryem.ennmily@gautiersemences.com (co-author)

Fatima Haj Ahmad, Al-Balqa Applied University, 19117 Al-Salt, Jordan; fatima.hajahmad@bau.edu.jo
(co-author)

Nihed Jerbi, Centre Régional de Recherche, en Horticulture et Agriculture Biologique, Sousse,
Tunisia; nihedjerbi12@hotmail.fr (co-author)

Asma Laarif, Centre Régional de Recherche, en Horticulture et Agriculture Biologique, Souss, Tunisia;
laarif.a@gmail.com (co-author)

Daniele Marian, CNR-IPSP National Research Council, Institute for Sustainable Plant Protection,
10135 Torino, Italy; daniele.marian@ipsp.cnr.it (co-author)

Slavica Matic, CNR-IPSP National Research Council, Institute for Sustainable Plant Protection, 10135
Torino, Italy; slavica.matic@ipsp.cnr.it (co-author)

Giuseppe Mazza, ESASEM S.p.A., 37052 Casaleone VR, Italy; gmazza@esasem.com (co-author)

Laura Miozzi, CNR-IPSP National Research Council, Institute for Sustainable Plant Protection, 10135
Torino, Italy; laura.miozzi@ipsp.cnr.it (co-author)

Abderrahmane Nazih, Laboratory of Soil Science, National Institute of Agricultural Research, 60000
Oujda, Morocco; abderrahmane.nazih@inra.ma (co-author)
Emanuela Noris, CNR-IPSP National Research Council, Institute for Sustainable Plant Protection,
10135 Torino, Italy; emanuela.noris@ipsp.cnr.it (co-author)

Francesco Nugnes, CNR-IPSP National Research Council, Institute for Sustainable Plant Protection,
80055 Portici NA, Italy; francesconugnes82@gmail.com (co-author)

Youness Taarabt, Laboratory of Soil Science, National Institute of Agricultural Research, 60000 Oujda,
Morocco; youness.taarabt@inra.ma (co-author)

Abdessalem Tahiri, Unité de Phytopathologie, Ecole National d'Agriculture, BP S 40 Meknès,
Morocco; atahiri@enameknes.ac.ma (co-author)

Cica Urbino, PHIM-CIRAD, Campus International de Baillarguet, 34398 Montpellier Cedex 5, France;
cica.urbino@cirad.fr (co-author)

Takoua Zaagueri, Institut National de la Rech. Agronomique, INRAT, 1004 El Menzah, Tunis, Tunisia;
takoua20@live.fr (co-author)

Michel Peterschmitt, Campus International de Baillarguet, CIRAD-PHIM, 34398 Montpellier Cedex 5,
France; michel.peterschmitt@cirad.fr (presenting author)

Anna Maria Vaira, CNR-IPSP National Research Council, Institute for Sustainable Plant Protection,
10135 Torino, Italy; annamaria.vaira@ipsp.cnr.it (co-author)

Abstract body text:

Geminiviruses (family Geminiviridae) are among the most damaging viruses in vegetable crops and particularly in the Mediterranean Basin. Through an international 3-year collaboration federated by a PRIMA call (Partnership for research and innovation in the Mediterranean area), a project involving public and private institutions from Italy, Jordan, Tunisia, Morocco, and France was launched on the prevention and control of new and invasive geminiviruses infecting vegetables in the Mediterranean. The prevalence of geminiviruses previously reported in this areas (i.e. tomato yellow leaf curl associated viruses, tomato leaf curl New Delhi virus, chickpea chlorotic dwarf virus, squash leaf curl virus and watermelon chlorotic stunt virus) has been monitored with virus-specific PCR tests on DNA extracted from symptomatic tomato and cucurbit plants. Meanwhile, the presence of potentially new geminiviruses circulating in the region is being investigated by deep-sequencing of DNA extracted from insects collected in the surveyed areas (mainly whiteflies, aphids, and leafhoppers). Harmonized procedures for collecting, storing, and analysing leaf and insect samples have been set up, allowing to compare results of virus incidence among countries. In 2020, more than one thousand leaf samples and about two hundred insect samples were collected in various agroecosystems as follows: Morocco, in Berkane area in the north-east and Souss region in the south; Tunisia, in Center, North, and Sahel regions; Italy, in Campania and Sicily; and in northern area of Jordan. Based on these surveys, the geminivirus prevalence in the Mediterranean Basin is being updated.